Synthesis Of Enediynes Research Articles

Enhanced resistance to heat and fungal infection in transgenic Trichoderma via over-expressing the HSP70 gene

Heat stress is one of the major abiotic stresses affecting the growth, sporulation, colonization and survival of Trichoderma viride. This study aimed to gain a better insight into the underlying mechanism governing the heat stress response of T. viride Tv-1511. We analysed the transcriptomic changes of Tv-1511 under normal and heat stress conditions using RNA sequencing. We observed that Tv-1511 regulates the biosynthesis of secondary metabolites through a complex network of signalling pathways. Additionally, it significantly activates the anti-oxidant defence system, heat shock proteins and stress-response-related transcription factors in response to heat stress. TvHSP70 was identified as a key gene, and transgenic Tv-1511 overexpressing TvHSP70 (TvHSP70-OE) was generated. We conducted an integrated morphological, physiological and molecular analyses of the TvHSP70-OE and wild-type strains. We observed that TvHSP70 over-expression significantly triggered the growth, anti-oxidant capacity, anti-fungal activity and growth-promoting ability of Tv-1511. Regarding anti-oxidant capacity, TvHSP70 primarily up-regulated genes involved in enzymatic and non-enzymatic anti-oxidant systems. In terms of anti-fungal activity, TvHSP70 primarily activated genes involved in the synthesis of enediyne, anti-fungal and aminoglycoside antibiotics. This study provides a comparative analysis of the functional significance and molecular mechanisms of HSP70 in Trichoderma. These findings provide a valuable foundation for further analyses.

ChemInform Abstract: A Novel Approach to the Synthesis of Enediynes.

Abstract A new method for the synthesis of enediynes is described based on stannylcupration of acetylene and certain activated alkynes, in situ reaction with 1-halo-1-alkynes, iododestannylation, and Pd(0)-catalyzed coupling with terminal alkynes.

Iodide acceleration in the Pd-catalyzed coupling of aromatic 1,2-ditriflates with alkynes: Synthesis of enediynes

Aryl enediynes have been synthesized in a one-pot procedure by a Pd-catalyzed coupling of terminal alkynes and ortho-aryl ditriflates. A variety of substrates have been examined.

ChemInform Abstract: New Dienes and Dienophiles. Part 5. Synthesis of Enediynes by Diels‐ Alder Addition.

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.

Synthesis of Enediynes by Diels–Alder Addition

Synthesis of Enediynes by Diels–Alder Addition

ChemInform Abstract: Synthesis of Enediynes by Reaction of Bicycloalkenyldiiodonium Salts with Lithium Alkynylcuprates.

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.

Synthesis of Enediynes by Reaction of Bicycloalkenyldiiodonium Salts with Lithium Alkynylcuprates

BicycloalkenyIdiiodonium salts, di[phenyl(trifluoromethanesulfonyloxy)iodo]norbornadiene (1a) and di[phenyl(trifluoromethanesulfonyloxy)iodo]-3,5-epoxy-1,4-cyclohexadiene (1b) react under mild conditions, with a number of lithium alkynylcuprates, generated in situ from the corresponding lithium acetylides and copper(I) cyanide, to give the respective enediynes 2 in moderate to good isolated fields