To address the problem of bacterial contamination in drinking water, we synthesized non-metallic photocatalysts for the inactivation of Escherichia coli in drinking water. In this paper, S-g-C3N4 (S-g-CN) composite nanomaterials were synthesized via a one-step calcination method. X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), electron paramagnetic resonance (EPR), and transmission electron microscopy (TEM) analyses confirmed the successful synthesis of S-g-CN with N vacancies. Scanning electron microscopy (SEM) and Fourier transform infrared (FTIR) spectroscopy analyzed the layered morphology and chemical structure of g-C3N4 before and after modification. Optical characterization revealed significantly enhanced light absorption, electron-hole separation efficiency, and carrier mobility in the modified samples. Bactericidal assays demonstrated that under visible light, 0.3 % S-g-CN nanomaterials exhibited superior bactericidal effects against E. coli compared to unmodified g-C3N4. Furthermore, 0.3 % S-g-CN demonstrated excellent degradation of Rhodamine B (RhB) under various conditions (pollutant concentration, pH, and catalyst dosage). Recyclability studies over three cycles confirmed the catalyst's stability and reusability.