Synbiotic Yogurt Research Articles

Grape pomace high-methoxyl pectin: A new prebiotic stabilizer for low-fat synbiotic yogurt gels – Optimization and characterization

High-methoxyl pectin (HMP, 72.5 % esterification degree and galacturonic acid content of 67.9 %) was extracted from grape pomace using a sequential ultrasound-microwave extraction. The extracted HMP was used to develop low-fat synbiotic set yogurts containing probiotic cells. Higher grape pomace pectin (GPP) concentrations (0.5–2 %) increased the probiotic bacterial population of Lactobacillus acidophilus LA-5 and Bifidobacterium bifidum BB-12. Higher cell viability was observed for L. acidophilus LA-5 compared to B. bifidum BB-12. A response surface optimization showed that the presence of 8.08 Log CFU mL−1L. acidophilus LA-5 and 1.88 % HMP experimentally resulted in the best probiotic viability (10.83 ± 0.11 Log CFU mL−1), overall acceptability (8.03 ± 0.06), and pH (4.25 ± 0.05) values. Compared to pectin-free probiotic yogurts, the optimal yogurt gels presented higher probiotic survivability, lower syneresis, and superior storage-dependent sensory attributes during 21 days of storage. However, a 14-day storage period was generally deemed suitable. The GPP-containing yogurt compared to the pectin-free sample exhibited higher colloidal stability with a larger particle size (433.8 nm vs. 272.5 nm) and lower zeta potential (−20.4 mV vs. −10.6 mV). Field emission-scanning electron (FE-SEM) and fluorescent (FLM) microscopy images confirmed a denser microstructure for GPP-enriched yogurts. The chemical interactions in the yogurt were not affected by enriching with GPP as investigated by FTIR, whereas the steady and dynamic rheological properties were significantly improved. GPP-enriched yogurt had a firmer gel structure with a larger linear region and lower G′ compared to the control, indicating a semi-solid state. The GPP as a multi-functional prebiotic ingredient would be promising in designing healthier food products.

Improvement of brown yogurt quality by Lactiplantibacillus plantarum S58 and oat β-glucan: Physicochemical properties, sensory quality, and metabolic changes

The flavor quality of brown yogurt is affected by the different fermentation methods. In this study, we investigated the effects of probiotics (Lactiplantibacillus plantarum S58), prebiotics (oat β-glucan), and synbiotics (Lactiplantibacillus plantarum S58 combined with oat β-glucan) on physicochemical properties, consumer acceptance, flavor substances, and metabolites of brown yogurt. The results showed that the addition of probiotics and synbiotics significantly shortened the curdling time of brown yogurt (30 min and 40 min, respectively), and synbiotics improved the physicochemical properties and flavor components of the yogurt. Compared with the control group, the volatile flavor substances in the synbiotic yogurt, such as acetophenone, 2-decanone, and octanoic acid, were significantly increased. Sensory analysis of 111 consumers showed that the sample of synbiotic brown yogurt was highly accepted and close to the ideal point. Synbiotic fermentation alters the metabolic profile of yogurt, increasing the levels of beneficial metabolites and reducing harmful metabolites. In conclusion, synbiotic brown yogurt achieved the highest quality. This study provides a theoretical basis and data support for the development of brown yogurt with high probiotic activity and a unique flavor.

Effect of the Fermentation Time of Purple Sweet Potato (Ipomea Batatas L) Synbiotic Yoghurt with Probiotic Starter on Product Quality and Antioxidant Activity

Purple sweet potato (Ipomea batatas L) has the potential as a fermentation medium because it has a high carbohydrate content of around 20.1%. The combination of purple sweet potato extract, skimmed milk, and probiotic starter is expected to be a synbiotic yogurt that can help the digestive system. This study aims to determine the effect of long-term fermentation of purple sweet potato synbiotic yogurt with probiotic starter culture on product quality and antioxidant activity. The observed quality was chemical, microbiological, and organoleptic, with variations in fermentation time of 0, 4, 8, and 12 hours. The study's results showed a long-term effect of the fermentation of purple sweet potato synbiotic yogurt with probiotic starter culture on the quality of yogurt. The variation of 0-hour fermentation time did not meet the pH chemical quality standard with a value of 5.16. Still, it met the TAT chemical quality standard with a value of 0.513%, while the variation of fermentation time of 4-12 hours met the pH quality standard with a value of 3.88-4.33 and a TAT of 1.268-1.967%. In the quality of microbiology, there was a total increase in BAL, which was 46.2×107 - 90.61×107. The best organoleptic quality of color, taste, and aroma was obtained in the 8-hour fermentation variation, while the best texture organoleptic quality was obtained in the 12-hour fermentation variation. The best antioxidant activity was received at 12 hours of fermentation with an IC50 value of 62.727 ppm. From this study, it can be concluded that the best yogurt results are obtained with a fermentation time of 12 hours.

Effect of Red Guava (Psidium guajava L.) Juice Concentration on Product Quality and Antioxidant Activity of Red Guava Synbiotic Yoghurt

Red guava juice has very strong antioxidant activity and has the potential to be processed into synbiotic functional beverage products that have health benefits. This study examined the effect of red guava juice concentration on product quality and antioxidant activity of red guava synbiotic yoghurt. Product quality tested included microbiological quality (total LAB) and chemical quality (pH value and Total Tritable Acid (TTA )value). The concentrations of red guava juice used were 0%, 10%, 20%, 30%, and 40%. Fermentation was carried out for 6 hours at 37⁰C by Lactic Acid Bacteria (LAB) from powdered mixed culture. Then organoleptic testing using the hedonic test, total LAB testing using the Total Plate Count (TPC) method, pH value testing using a pH meter, TTA value testing using the acid-base titration method, and antioxidant activity testing using the DPPH (2,2-diphenyl-1-picrylhydrazil) method. Test data were analyzed using SPSS software to determine the effect of red guava juice concentration on test results. The concentration of red guava juice in red guava synbiotic yoghurt affects the pH value, TTA value, and IC50 value as the result of antioxidant activity testing. Red guava juice concentration did not affect total LAB testing. Organoleptic assessment of red guava symbiotic yoghurt is in the range of like to like very much. The best formulation was red guava synbiotic yoghurt with 30% red guava juice concentration, which had strong antioxidant activity with an IC50 value of 59.637 ppm, pH value of 3.94, TTA value of 1.98%, and total LAB as much as 1.81x10⁸ colonies/g, so it has met the quality requirements of yoghurt according to Indonesian National Standard SNI 2981: 2009.

Consumption of a new developed synbiotic yogurt improves oxidative stress status in adults with metabolic syndrome: a randomized controlled clinical trial

Association between metabolic syndrome (MetS) and oxidative stress has been shown in numerous studies. It has been shown that probiotics could be the effective treatment strategy in improving oxidative stress. This study aimed to determine the effects of a new developed synbiotic yogurt on oxidative stress status in adults with MetS. Forty-four individuals were assigned into two groups and given 300 g of synbiotic yogurt containing Lactobacillus plantarum, Lactobacillus pentosus, and Chloromyces marcosianos yeast or regular yogurt for 12 weeks in this randomized, placebo-controlled clinical trial. Before and after the intervention, biochemical parameters were assessed. Daily consumption of synbiotic yogurt in adults with MetS showed a statistically significant improvement in the level of glutathione peroxidase (p = 0.01) and total oxidant status (p = 0.006) compared to the regular yogurt. Total Antioxidant Capacity and superoxide dismutase levels increased significantly (p = 0.002 and p = 0.02, respectively) in the intervention group compared to the baseline levels. In adults with MetS, daily consumption of the synbiotic yogurt containing native strains of Lactobacillus plantarum, Lactobacillus pentosus, and Chloromyces marcosianos yeast for 12 weeks was associated with improvements in oxidative stress status.Trial registration number: Iranian Registry of Clinical Trials (IRCT20220426054667N1) (18/05/2022)

Isolation and characterization of probiotic lactic acid bacteria from local yogurt and development of inulin-based synbiotic yogurt with the isolated bacteria

Synbiotic yogurts can offer several potential benefits due to the combination of yogurts probiotics, prebiotics, and the nutritional qualities of milk. Local yogurt variants might add to the variety of probiotic alternatives because they frequently have distinct microbial communities. Employing indigenous strains not only represents cultural preferences but also increases the probability of obtaining bacteria adapted to the surrounding environment. This study was intended to isolate and characterize potential lactic acid bacterial strains from local yogurt and assess the effectiveness of inulin on the physicochemical and microbiological properties during the fermentation of synbiotic yogurt. A total of eight strains were identified and isolated. Two chosen isolates were combined with inulin at 0, 0.5, 1, and 1.5 % concentrations to produce synbiotic yogurts. The physicochemical and microbiological properties were assessed during the fermentation process of synbiotic yogurts. The result showed that the isolated Lactobacillus spp. and Streptococcus spp. strains had probiotic characteristics and good growth. After the fermentation, the yogurt sample containing 1.5 % inulin had the highest count of Lactobacillus spp. (8.17 ± 0.01 log CFU/ml) and Streptococcus spp. (8.03 ± 0.03 log CFU/ml), highest optical density (2.931 ± 0.001), acidity (1.43 ± 0.03), and water-holding capacity (63.54 ± 0.51), and lowest syneresis (34.75 ± 0.48). The addition of inulin did not affect color during the fermentation of synbiotic yogurt. This study concluded that inulin could boost yogurt's stability when combined with local lactic acid bacteria strains.

The Effect of Lactiplantibacillus plantarum SHY130 and Konjac Glucomannan on the Physicochemical, Antioxidant, and Sensory Properties of Stirred Yogurt.

The impact of konjac glucomannan (KGM)-based synbiotics on yogurt quality is not well understood. This study investigated the effects of a synbiotic mixture of KGM and the selected probiotic Lactiplantibacillus plantarum SHY130 on the physicochemical, antioxidant, and sensory properties of yogurt. The results showed that KGM significantly promoted the growth of Lactiplantibacillus plantarum SHY130. The synbiotics dramatically enhanced the count of lactic acid bacteria in yogurt during the 14 days of storage. Texture analysis indicated that the synbiotic supplement had no impact on springiness and cohesiveness but resulted in notable reductions in hardness, gumminess, and chewiness. The synbiotics did not significantly affect the water-holding capacity and syneresis. While the synbiotics initially decreased yogurt viscosity, it increased with storage time. Furthermore, the synbiotics significantly improved the yogurt's antioxidant capacity. Additionally, the supplementation of the synbiotics did not adversely affect sensory properties, although the synbiotics containing 0.02% KGM negatively impacted overall acceptability. Overall, these findings elucidate the effects of KGM-based synbiotics on yogurt quality, providing a foundation for developing novel synbiotic yogurt products.

Development and Performance Evaluation of Synbiotic Red Banana Yoghurt

Aims: The present study aimed at the production of yoghurt with different levels of red banana pulp (5, 7.5, 10, 12.5, 15%) Study Design: Development of red banana pulp incorporated probiotic yoghurt and its performance evaluation. Place and Duration of Study: Department of Dairy Microbiology, Dairy Science College, Hebbal, Bengaluru, Karnataka Veterinary, Animal and Fisheries Sciences University, Bidar, Karnataka, India. Between June 2022 and March 2023. Methodology: The red banana pulp incorporated probiotic yoghurt was developed and subjected to sensory analysis by the panel of judges using 9-point hedonic scale, analyzed for milk set time, acidity and viability and the best combination was selected. Results: The 10% concentration of red banana pulp was selected which showed 2.3 h of milk set time, acidity of 0.74% LA, viable log count/g was 9.50 and overall acceptability score was 8.25. The contaminants like coliforms and yeast and molds were absent. The 10% concentration showed no significant difference (P =.05) with the control sample in milk set time and acidity, while significant difference was observed in viability among the different concentrations. The samples were analyzed for synerisis which was 9.30%, water activity was 0.939, viscosity was 6.08cP and color intensity L* was 80.17, a* was -0.85 and b* was 8.68, for the developed red banana pulp incorporated probiotic yoghurt. Conclusion: The developed product improves the acceptability and provides benefits of both probiotics and red banana for the consumers.

Antioxidant activity and viability Lactobacillus acidophilus synbiotic yogurt with the addition of white oyster mushroom (Pleurotus ostreatus) extract

Antioxidant activity and viability Lactobacillus acidophilus synbiotic yogurt with the addition of white oyster mushroom (Pleurotus ostreatus) extract

Effect of The Addition of Unripe Berlin Banana Flour on The Organoleptic Characteristics of Synbiotic Yogurt

Synbiotic yogurt is a fermented milk product that combines probiotic bacteria with ingredients containing prebiotic components. Unripe banana flour (UBF) contains resistant starch (RS), which contributes to the development of prebiotics in synbiotic yogurt products. The research aimed to know the effect of the addition of UBF on the organoleptic characteristics of synbiotic yogurt. The research was carried out with an unripe Berlin banana. Four treatment groups included 2% inulin (P0), 1% UBF (P1), 2% UBF (P2), and 3% UBF (P3). The organoleptic characteristics include hedonic and hedonic quality tests using 48 semi-trained panelists. The data was statistically analyzed using the Kruskall-Wallis test followed by the Mann-Whitney test. The results of the hedonic test indicate that texture, aroma, and color significantly differed between groups (P<0.05), while taste did not significantly differ between groups (P>0.05). The hedonic quality test indicated that there were significant differences between groups in terms of texture, aroma, and color (P<0.05). However, there was no significant difference in taste between the groups (P>0.05). In conclusion, formula 2% UBF (P1) is the most preferable formula based on the organoleptic test.

The role of inulin extract from mangrove apple (Sonneratia caseolaris) and Lactobacillus plantarum combination as a synbiotic

Synbiotic yogurt products can be developed using mangrove apple extract inulin as a prebiotic in combination with Lactobacillus plantarum to form a probiotic. Therefore, this study aimed to determine the optimal concentrations of inulin extracted from mangrove apple for the viability of L. plantarum as a synbiotic in vitro and its antibacterial activity against Staphylococcus aureus. The samples were divided into 5 groups and 4 replicates consisting of different concentrations of inulin extracted from mangrove apple (IEMA) at 0, 3, 6, 9, and 12% w/v, which was combined with L. plantarum as a synbiotic. The result showed that the addition of IEMA with different concentrations had a significant effect (p<0.05) on the viability of L. plantarum in gastric juice and bile salt after 5 h of exposure. The total bacteria significantly decreased (p<0.05) after 4 weeks of storage period. The antibacterial activity of IEMA at concentrations of 9% and 12% was higher than other treatments as demonstrated by p<0.05. IEMA increased the viability with the concentration of 9% being the most effective (p<0.05) on acid, bile salt, resistance, and storage periods. Based on the results, IEMA can also inhibit the growth of S. aureus.

Dextrose Equivalent (DE) Variation and Maltodextrin Concentration Effects in Yoghurt Powder Characteristics Using Foam-mat Drying

Lactic Acid Bacteria (LAB) in yoghurt is a probiotic bacteria which potentially improves intestinal health. Yoghurt has a high moisture content. Thus, storing it at low temperatures can maintain the stability of microbes in the yoghurt. Yoghurt stored in the refrigerator has a maximum shelf life of 10 days. Yoghurt powder is one of the alternative solutions to prolong the shelf life. This study used a randomized design of factorial groups with variations of dextrose equivalent (DE) values of 10-12 and 18-20, as well as variations of maltodextrin concentrations of 10, 15, and 20%. The study aimed to determine the DE value and maltodextrin concentration which can produce the best quality synbiotic yoghurt powder. The quality of this synbiotic yoghurt was assessed by the highest yield, lowest water content, highest solubility, highest water absorption, highest acidic titrated total, highest total of lactic acid bacteria, and highest organoleptic. The study obtained that the DE value of maltodextrin affected the total LAB. Meanwhile, the concentration of maltodextrin affected the yield, moisture content, solubility, acidic titrated total, and total of LAB. The interaction between the DE value and the concentration of maltodextrin did not affect all the parameters of this study.

The effect of aqueous extract of Arctium lappa root on the survival of Lactobacillus acidophilus La-5 and Bifidobacterium bifidum Bb-12 and sensorial and physicochemical properties ofsynbiotic yogurt.

The effect of aqueous extract of Arctium lappa root (ALE) on the survival of Lactobacillus acidophilus La-5 and Bifidobacterium bifidum Bb-12 probiotic bacteria and sensory and physicochemical properties of synbiotic yogurt was evaluated during 4 weeks storage at 4°C. According to this study, using 0.5% and 1% ALE significantly affected the survival of La-5 and Bb-12 during storage. The results showed that 1% of ALE counting of La-5 and Bb-12 has been reached from 6.96 and 8.14 Log CFU/g to 7.3 and 7.30 Log CFU/g after 28 days of storage. Moreover, adding 1% ALE to yogurt enhanced antioxidant activity and phenolic content to 1299.8 mg gallic acid/kg and 392.8 mg BHT eq./kg compared with the control (without extract) after storage, respectively. In general, in yogurt containing ALE, a decrease in Syneresis, undesirable changes in taste, texture, and appearance, and reduced overall acceptances were observed compared to the control. In conclusion, using this prebiotic compound (ALE) can improve nutritional properties and probiotic protection in yogurt during long time storage; thus, it is a good choice for application in the dairy industry.

Kinetics of Formation of Butyric and Pyroglutamic Acid during the Shelf Life of Probiotic, Prebiotic and Synbiotic Yoghurt

Butyric acid (C4) and pyroglutamic acid (pGlu) exert significant beneficial effects on human health. In this study, the influence of probiotics (Lactobacillus acidophilus and Bifidobacteria) and/or prebiotics (1 and 3% inulin and fructo-oligosaccharides) on the content of C4 and pGlu in yoghurt during the shelf-life period was evaluated. The contents of C4 and pGlu were determined in probiotic, prebiotic and synbiotic yoghurts during 30 days of storage at 4 °C by solid-phase microextraction coupled with gas chromatography/mass spectrometry and HPLC analysis. Traditional yoghurt and uninoculated milk were used as control. Prebiotic yoghurt contained more C4 (2.2–2.4 mg/kg) than the uninoculated milk, and no increase was detected with respect to traditional yoghurt. However, probiotic yoghurt showed 10% more C4 than traditional yoghurt. Adding fibre to probiotics (synbiotic yoghurt) the C4 content increased by 30%. Regarding pGlu, probiotic yoghurt presented the highest content of approximately 130 mg/100 g. Fibre did not affect pGlu content. Finally, C4 and pGlu contents generally increased up to 20 days of storage and then decreased up to 30 days of storage. The results might be useful for the preparation of other functional foods rich in C4 and pGlu using lactic acid bacteria.

Functional improvement of synbiotic yogurt enriched with Lacticaseibacillus rhamnosus and aloe vera gel using the response surface method

The response surface technique was applied to produce synbiotic yogurt containing Lacticaseibacillus rhamnosus and aloe vera gel (AVG) with high functionality (antioxidant and antimicrobial characters), superior physicochemical properties, and desirable sensory attributes. The experiments were planned around a central composite design (CCD) with two independent variables: AVG concentration (0–5%, w/w) and storage time (1–28 days). The AVG concentration and storage time significantly improved the viability of L. rhamnosus up to 7.9 cfu/g during the shelf life which is a practical limit for a probiotic. It enhanced the yogurt’s antioxidant and antipathogenic activity, proteolytic content, water-holding capacity, and sensory aspects. High concentrations of AVG reduced the yogurt’s desirable textural aspects (hardness and gumminess) except for firmness and adhesiveness and to some degree the sensory properties as well. The results showed that adding 5% AVG to probiotic yogurt produced a functional food with 68% desirability that retained its beneficial properties for at least 14 days under refrigerated storage.Graphical

LACTOSE-FREE ICE CREAM TECHNOLOGY BASED ON SECONDARY DAIRY RAW MATERIALS

Expanding the assortment of lactose-free dairy products, namely, ice cream for people who cannot tolerate lactose is a promising direction of research. The purpose of the work is to develop the technology of milk lactose-free synbiotic yogurt ice cream based on buttermilk protein-lipid concentrate, obtained by ultrafiltration/diafiltration, with the addition of natural flavoring and food additives, and the study of its quality indicators. A protein-lipid concentrate of buttermilk with a low lactose content was obtained by ultrafiltration/diafiltration, and its quality indicators were determined. The concentrate has a high protein content (16.01%) and a low lactose content (1.28%). Protein-lipid concentrate fermented with synbiotic sourdough is the main component included in the formulation of lactose-free synbiotic yogurt ice cream. The main physico-chemical parameters of the obtained fermented protein-lipid concentrate were determined, which ensures a rational content of the main components of the chemical composition in the finished lactose-free ice cream (it does not contain lactose, the protein content is 8.46%) and a high content of useful microflora (bifido- and lactobacilli). A mixture for lactose-free synbiotic yogurt ice cream was obtained according to a scientifically based recipe. A sample of lactose-free ice cream was made from the mixture using traditional technology, and organoleptic, physico-chemical and microbiological quality indicators were investigated. A comparison of the quality indicators of the studied sample with the control, which was taken as classic yogurt ice cream, was carried out. The mass fraction of lactose in samples of lactose-free ice cream is 0,011%. The developed lactose-free synbiotic yogurt ice cream has high probiotic activity (contains lacto- (3.0•108 CFU/g) and bifidobacteria (3.5•109 CFU/g)).

Antioxidant, Syneresis, and Sensory Characteristics of Probiotic Yogurt Incorporated With Agave tequilana Aqueous Extract

Background: The growing consumer interest in healthy food has encouraged the industry to search for products that exhibit additional benefits over nutritional value. Yogurt is the best carrier for beneficial nutrients such as probiotics and antioxidants. Agave tequilana is a plant that contains phytochemicals comprising flavonoids that show antioxidant activity. Objectives: In this work, the effects of incorporating A. tequilana aqueous extract (ATAE) on the antioxidant properties, syneresis, and flavor of probiotic yogurt were investigated. Methods: Radical scavenging ability was evaluated against the 2,2-diphenyl-1-picrylhydrazyl (DPPH). Amounts of total phenolic compounds (TPC) were determined using the Folin-Ciocalteu method. Syneresis was determined using the centrifugal technique. The sensory evaluation was carried out using a 5-point hedonic scale. Results: The addition of ATAE in yogurts exhibited a dose-dependent relationship and had significantly (P≤0.05) higher TPC and DPPH scavenging ability than the control yogurts. The TPC and DPPH scavenging properties of 0.5%, 1%, and 1.5% ATAE-fortified yogurts were 389.9, 629.2 and 905.6 mg GAE/kg yogurt and 283, 480, and 617 mg BHT eq./kg yogurt, respectively. The addition of ATAE increased the syneresis of yogurt samples (P≤0.05). Although the sensory properties of synbiotic yogurt samples were lower than plain and probiotic treatments, their scores were still above the acceptable level. Conclusion: As the results of this study indicated, it is recommended to improve the antioxidant properties of the yogurt by incorporating AEAT and its potential application as a functional food formulation.

Physicochemical, sensory and microbiological characteristics of coriander seed powder yogurt

Yogurt is a fermented food obtained by the bacterial fermentation of milk. In the present work, the effect of different concentrations (1, 3 and 5% w/w) of coriander (Coriandrum sativum) seed powder on physicochemical, sensory characteristics and viability of Bifidobacterium bifidum and Lactobacillus acidophilus of probiotic yogurt were investigated at 4 °C for 21 days. Laboratory-made yogurts were obtained by inoculating milk with yogurt bacteria (mixed culture of Streptococcus thermophilus and Lactobacillus delbrueckii ssp. bulgaricus) and two probiotic cultures (Lactobacillus acidophilus and Bifidobacterium bifidum). According to the results, the viability of B. bifidum and L. acidophilus increased in synbiotic stirred yogurts with 5% CSP (coriander seed powder) up to 9.15 ± 0.09 log CFU/g at 11 days of the storage period, whereas probiotic bacteria count decreased to 9.02 ± 0.01 by the end. Therefore, our results confirmed that the addition of probiotics and CSP powder improved the physicochemical and sensory characteristics of stirred yogurt and exerted a beneficial effect on probiotic bacteria.

The effect of a new developed synbiotic yogurt consumption on metabolic syndrome components, oxidative stress status, and some other cardiovascular disease risk factors in adults with metabolic syndrome: a study protocol for a randomized clinical trial

BackgroundMetabolic syndrome is recognized as one of the most common global health issues, which may cause numerous side effects. Studies have shown the favorable effects of probiotic supplements on glycemic indices, lipid profiles, and oxidative stress status. However, the number of studies investigating the effects of food products containing probiotics and prebiotics on metabolic diseases is limited. Limited evidence also shows that products containing Lactobacillus plantarum could affect metabolic alterations in chronic diseases. No previous study evaluated the impact of synbiotic yogurt containing Lactobacillus plantarum on people with metabolic syndrome. Therefore, the current study aims to investigate the effect of the newly developed synbiotic yogurt containing Lactobacillus plantarum, Lactobacillus pentosus, and Chloromyces marcosianos yeast on the components of metabolic syndrome, oxidative stress status, and some other risk factors for cardiovascular diseases in adults with metabolic syndrome.MethodsIn this study, 44 patients with metabolic syndrome will be randomly assigned to intervention and control groups in a randomized, double-blind, controlled clinical trial. Participants in the intervention group will consume 300 g of synbiotic yogurt daily, while those in the control group will consume 300 g of regular yogurt daily for 12 weeks. Anthropometric measurements, blood pressure, and biochemical parameters will be evaluated before and after the intervention.DiscussionThe management of the metabolic syndrome presents significant clinical challenges. While probiotic supplementation for these individuals has been considered, the consumption of probiotic-rich foods has received considerably less attention.Trial registration numberIranian Registry of Clinical Trials (IRCT20220426054667N1) (2022–05-18).

Product development of Amorphophallus muelleri Bl. Synbiotic yogurt using Quality Function Deployment method

The COVID-19 pandemic has significantly changed and impacted various aspects of life. The customer behavior of the Indonesian people has changed during this pandemic. Increasing public awareness of health is an opportunity to develop functional foods which can improve the body’s immune system. Some probiotic dairy products, e.g., yogurt, are currently being developed with the addition of prebiotics and are also known as synbiotic yogurt. This research aims to develop an Amorphophallus muelleri Bl. Synbiotic yogurt product design based on customer requirements and engineering characteristics. The method used in this study is the Quality Function Deployment (QFD) method. Quality Function Deployment is a method to develop product design quality to achieve customer satisfaction. The results showed that the product attributes customers want for yogurt include a rather strong aroma, a slightly sour and sweet taste, a thick texture, and a production volume of 250 ml packaged in cup packaging.