In the context of the demand for mass customization of products, a trade-off between highly efficient automated systems and flexible manual operators is sought. The linear arrangement of workstations made it possible to divide the process into many simple operations, which increases production efficiency, but also results in an increase in the number of workstations and a significant extension of the line. A human operator is usually treated as a quasi-mechanical object, and a human error is considered, similarly, as a failure of a technical component. However, human behavior is more complex and difficult to predict. A mathematical model of a new production organization is presented, including dividing the traditional production line into shorter sections or replacing the serial assembly line with a U-line with cells. Moreover, the reliability of operator and technical means are distinguished. Work-in-progress inventories are located between line sections to improve system stability. The stability of the assembly line is examined based on the system configuration and probabilistic estimates of human failure. The influence of the symmetry of reliability parameters of people on key performance indicators (KPI (headcount), KPI (surface) and KPI (Overall Equipment Effectiveness) is examined. KPI (solution robustness) and KPI (quality robustness) are also presented in order to evaluate the impact of a disruption on the assembly line performance. New rules for assigning tasks to stations are proposed, taking into account the risk of disruptions in the execution of tasks. For comparison of assembly problems, heuristic methods with newly developed criteria are used. The results show the impact of symmetry/asymmetry on assembly line performance and an asymmetric distribution of manual assembly times that is significantly skewed to the right due to human errors. On the assembly line, the effects of these errors are cumulative and lead to longer assembly times and lower KPIs.