Symbiotic Microflora Research Articles

Dysbiotic changes in the intestines under the influence of exogenous biological determinants

Introduction. Data were obtained on the difference in the quantitative and specific composition of intestinal microbiota bacteria in practically healthy individuals with subclinical invasions by pathogenic intestinal protozoa Lamblia intestinalis, Blastocystis hominis. Сhanges in bacterial composition were shown to have characterological features and species-specific «targets» of intestinal microbiota destruction. 
 Material and methods. Bacteriological and parasitological research methods were used. For a comparative analysis of quantitative and specific changes in the symbiotic microflora, there were formed two groups: “Yes” and “No” for each type of pathogenic protozoa, bacteriological indicators were ranked by quantitative content — CFU “0–103”, CFU “103–10max”, CFU “10max”. Statistical differences in research results were determined by the Chi-square calculation method. 
 Results. Antagonistic activity of unicellular protozoa in relation to the symbiotic bacterial intestinal microflora in the examined population groups has a different degree of severity depending on the type of invasion. Parasitic pathogens were shown to inhibit up to 40% of the quantitative and 50% of the species composition of the intestinal microflora and are an aggressive biogenic factor in the destruction of biota. There are no limitations of the study. 
 Limitations. The studies had no methodological or administrative restrictions.
 Conclusion. For the first time, significant statistical material has confirmed that asymptomatic parasitosis is a strong biogenic determinant of the development of destructive changes in the integral structure of the symbiotic microflora and the formation of a subclinical form of dysbiosis in an “almost healthy contingent” of the population. The asymptomatic carriage of Blastocysts spp. was found to have a more pronounced antagonistic effect on the bacterial composition of the intestinal microbiota than the presence of L. intestinalis, Blastocysts spp., form intestinal dysbiosis more aggressively, which indicates a greater pathogenic potential of B. hominis in the formation of intestinal pathology in humans.

Foreign research experience of the effect of probiotic agent on the intestinal microbiome of poultry

The intensive development of industrial animal husbandry and poultry farming, along with the ban on the use of antibiotics for non-medicinal purposes, requires a revision of stimulating and therapeutic and preventive approaches aimed at increasing productivity, protecting against infections and improving other production parameters, such as feed digestibility and improving the efficiency of digestion, stimulating growth and development, the quality of meat, milk, eggs. In this regard, probiotic, prebiotic and synbiotic drugs and feed additives have become widespread. Funds based on antagonistic bacteria and representatives of symbiotic microflora are actively being introduced into poultry technology, a wide domestic research experience reveals their prospects. Used mainly to maintain the balance of the intestinal microbiota, they turn out to be an effective method of combating opportunistic pathogens.At the same time, in the countries of developed industrial poultry farming, products based on probiotic cultures have not been widely used, and a number of sources are skeptical about the ability of probiotics to influence the intestinal microbiome of poultry. This article discusses various modern studies that carry the opinions of foreign authors about the effectiveness of probiotics and their effect on the intestinal microbiome of poultry.

Формирование патогенного потенциала энтробактерий в ассоциативном симбиозе кишечника пчел

Enterobacteria, being permanent inhabitants of the gastrointestinal tract of bee individuals, play a significant role in maintaining homeostasis of the macroorganism. For the life of the bee family, the symbiotic microflora is important, because due to bacterial enzymes of glycosidases, carbohydrates are broken down and nectar is converted into honey, protein components of the feed are digested [2], and protection from pathogenic microorganisms is carried out [13]. However, a number of researchers report on the pathogenicity of representatives of the Enterobacteriaceae family for bees. In this regard, we conducted comparative studies to identify the relationship between the state of the intestinal intestines of honeybees and their physiological activity.

ОБМЕН ВЕЩЕСТВ В ОРГАНИЗМЕ ОВЕЦ ПРИ ИСПОЛЬЗОВАНИИ МЕТИОНИНА В РАЗНЫХ ФОРМАХ

A comparative study of the feeding of native (M) and the developed “protected” form of methionine (MP) to sheep revealed a more pronounced positive effect of it in the form protected from the indirect impact of symbiotic microflora on the direction of metabolic processes in the body. The application of the developed method led to a significant improvement in a number of indicators of protein and carbohydrate-fat metabolism, the functional activity of the liver

Influence of intestinal microbiota on the immunopathogenesis of atopic dermatitis in children

Particular attention is paid to atopic dermatitis (AD) as one of the earliest and most frequent clinical manifestations of allergy in children. AD is a multifactorial disease, the development of which is closely related to genetic defects in the immune response and adverse environmental influences. It was found that the action of these factors determines the rate of development of AD, especially in young children. One of these factors is a violation of the intestinal microbiota, which plays an essential role in the development of the child's immune system and has a protective effect in the formation of atopy. It has been shown that 80-95% of patients with AD have intestinal dysbiosis, while, along with a deficiency of lactobacilli and bifidobacteria, there is an excessive growth of Staphilococcus.
 The use of modern molecular genetics technologies made it possible to obtain a fairly complete understanding of the number, genetic heterogeneity and complexity of the bacterial components of the intestinal microbiota, while clinical studies have shown the importance of its interactions with the host organism in the formation of various forms of pathology. It has been established that the human intestinal microbiota is an evolutionary set of microorganisms that exists as a balanced microecological system in which the symbiotic microflora is in dynamic equilibrium, forms microbial associations that occupy a certain ecological niche in it, and is one of the most important factors affecting human health.
 The gut microbiota plays an important role in the pathogenesis of atopic dermatitis, which causes immunosuppression, but the exact mechanism of its action is still unclear. It is widely known that probiotics act on the immune system. These are living microorganisms with immunomodulatory effects that stimulate Th1 cytokines and suppress Th2 responses, which are being investigated for the treatment of several diseases. The most commonly used probiotics are part of the intestinal microflora such as lactobacilli, bifidobacteria and enterococci.
 The purpose of this article: to systematize the information available today on the influence of the composition of the intestinal microflora on the immunopathogenesis of atopic dermatitis.

Targeted Development of Infant Microbiota on Formula Feeding: Modern Options

The review provides the analysis of recent publications covering the biological significance of infant microbiome and showing modern research technologies. Correlations of quantitative and qualitative characteristics of intestinal microbiota with delayed health issues were evaluated. Various concepts of newborn microbiome origin, timing, and stages of bacterial colonization of gastrointestinal tract were considered. Data on the role of delivery method on infant microbiota development is presented (including the significance of contamination with maternal vaginal flora where ontogenetic priority belongs to lactobacilli). It was revealed that Lactobacillus fermentum was the basis microflora providing the conditions for the subsequent successful colonization of the child’s intestines with obligate symbionts. The significance of breastfeeding in adequate microbiota development was confirmed. In case of mandatory formula feeding, it is necessary to enrich infant milk formulas with components that promote the selection of symbiotic microflora such as: long-chain polyunsaturated fatty acids, prebiotics, oligosaccharides, and probiotics of specific action. The innovative formula based on synbiotic/combiotic concept will be able to partially compensate impaired microbiota development in infant on mandatory formula feeding.

PECULIARITIES OF DENTAL CARIES PREVENTION IN CHILDREN WITH OLIGOPHRENIA

Nowadays dental caries and periodontal diseases are among the most pressing problems in Paediatric Dentistry. Young patients with psychoneurological disorders are a special category among children with somatic pathology. At the same time, the issue of providing dental care to such children is underrepresented. Today, traditional schemes used for the prevention and treatment of caries and periodontal disease in children with mental retardation requires further improvement taking into account the clinical status of children with particular forms of mental retardation, including oligophrenia. Thus, the aim of our study was to develop and evaluate the effectiveness of a complex of measures to prevent dental caries in children with oligophrenia.
 To assess the effectiveness of the elaborated preventive complex, 56 children aged 12 years with the diagnosis of oligophrenia of I degree of severity (mild mental backwardness) were under observation during 2 years; 27 children received the complex we developed. The group of comparison included 29 children; caries prevention measures for these children were based on regional protocols (hygienic education, keeping oral hygiene, use of fluoride toothpastes).
 The effectiveness of the developed preventive complex was evaluated 6, 12 and 24 months after the beginning of its implementation according to the following criteria: the growth of dental caries intensity; reduction in the growth of dental caries intensity; level of oral hygiene (OHI-S index); changes in the biocenosis of the oral cavity (in 1 and 6 months of the observation).
 The results of the examination after the 6 month observation period have shown the dental caries intensity in children of the main group is 6.65 ± 0.45 teeth that is 1.02 times lower than in the children of the comparison group (6.79 ± 0.26 teeth, p ˃0.05). Thus, the growth of dental caries intensity in the children of the main group is, on average, 0.41 ± 0.05 tooth, which is significantly lower than in children of the comparison group (0.58 ± 0.06 tooth, p <0.05 ).
 The reduction in the growth of caries intensity in children who received the developed preventive complex was 41.46%. Thus, during two years of the observation, the growth of caries intensity in children of the main group is 1.52 ± 0.08 tooth that is 1.74 times lower than in children of the comparison group (2.64 ± 0.9 tooth, p <0, 05). The reduction in the growth of caries intensity in children who received this preventive complex is, on average, 42.42%.
 The effectiveness of the proposed preventive complex for children with oligophrenia and carious lesions was also evaluated by using the hygienic Green-Vermillion index. According to the data obtained, at the period of inclusion into the study, the children in both groups demonstrated nearly the same indices of the oral hygiene. However, over the period of the study, the indices of the hygiene index between the children of the main group and the comparison group differed significantly. The application of preventive complex in children with oligophrenia resulted in better oral hygiene than in that the children of the comparison group. This can be explained by professional hygiene, quality control of toothbrushing and hygiene education of children and their parents. The effectiveness of the elaborated complex of preventive measures was also evidenced by changes in the oral biocenosis in the children of the main group in 1 and 6 months of the observation. The findings of microbiological studies showed that the microbial spectrum of the oral cavity changed toward the healthier balance in the children of the main group during the observation period: there was gradual decrease in pathogenic microflora and an increase in symbiotic microflora.
 Thus, the analysis of findings obtained over the two year observation period in children with oligophrenia, who received the complex of measures to prevent dental caries prevention has confirmed its effectiveness by reducing the growth of caries intensity, the reduction in the growth of caries intensity, hygiene improvement and normalization of oral microbiocenosis.

Gut Microbiota and Their Role in Health and Metabolic Disease of Dairy Cow.

Ruminants are mostly herbivorous animals that employ rumen fermentation for the digestion of feed materials, including dairy cows. Ruminants consume plant fibre as their regular diet, but lack the machinery for their digestion. For this reason, ruminants maintain a symbiotic relation with microorganisms that are capable of producing enzymes to degrade plant polymers. Various species of microflora including bacteria, protozoa, fungi, archaea, and bacteriophages are hosted at distinct concentrations for accomplishing complete digestion. The ingested feed is digested at a defined stratum. The polysaccharic plant fibrils are degraded by cellulolytic bacteria, and the substrate formed is acted upon by other bacteria. This sequential degradative mechanism forms the base of complete digestion as well as harvesting energy from the ingested feed. The composition of microbiota readily gets tuned to the changes in the feed habits of the dairy cow. The overall energy production as well as digestion is decided by the intactness of the resident communal flora. Disturbances in the homogeneity gastrointestinal microflora has severe effects on the digestive system and various other organs. This disharmony in communal relationship also causes various metabolic disorders. The dominance of methanogens sometimes lead to bloating, and high sugar feed culminates in ruminal acidosis. Likewise, disruptive microfloral constitution also ignites reticuloperitonitis, ulcers, diarrhoea, etc. The role of symbiotic microflora in the occurrence and progress of a few important metabolic diseases are discussed in this review. Future studies in multiomics provides platform to determine the physiological and phenotypical upgradation of dairy cow for milk production.

Gut modulation of dysbiosis induced by dextran sulfate sodium

Inflammatory bowel disease is one of the serious burdens of clinical medicine and healthcare. This study investigated the potential of a biological product based on mare's milk and metabolites of symbiotic microflora for modulation of intestinal microflora affected by dextran sulfate sodium (DSS)-induced dysbiosis. Symbiotic microflora was isolated from the stool of healthy volunteers. Lysates for the production of short-chain fatty acids of screened microorganisms were mixed with mare's milk. The activity of the biological product was evaluated on the DSS model of induced colitis. Histological changes in the intestinal epithelium were determined. The structure of the microbiome was evaluated based on the analysis of 16S rRNA microbial sequences. Histological examination of rat intestinal tissues after application of the biological product showed reduced infiltration of granulocytes, macrophages, and lymphocytes. The results of sequencing demonstrated a decrease in the biological diversity of microbiota affected by colitis. The full recovery was observed after 21 days of the application of the biological product. The product induced the structural changes of the microbiome damaged by DSS. Likewise, the number of the pathogenic intestinal microflora was decreased Representatives of SCFA producing bacteria increased concentrations of genus Lactobacillus.

Влияние пробиотика «Витацелл» на симбиотическую микрофлору рубца и биохимический статус телят

Biological feed additive "Vitacell" belongs to the group of biologically active metabolites containing live vegetative cells and spores of microorganisms of cellulolytic, probiotic and prebiotic action. The inclusion of the probiotic "Vitacella" in the diet of young cattle contributes to the stimulation of metabolic processes in the body, increasing feed conversion and improving clinical and biochemical blood parameters. The effectiveness of the use of such feed additives has been clinically proven by domestic and foreign authors. The use of these drugs helps to normalize the intestinal microflora, maintain mineral homeostasis and, as a result, increase the immune reactivity of the body, which is an integral part of the successful management of animal husbandry in the modern conditions of the food market. Keywords: PROBIOTIC, VITACELL, SYMBIOTIC MICROFLORA, BIOCHEMICAL STATUS

Progress of researches on symbionts in the management of mosquito-borne infectious diseases

Mosquitoes are the main vectors of many infectious diseases, including malaria and yellow fever, which seriously threaten human health across the world. In addition to the use of chemical insecticides, genetic control is a new attempt to currently available interventions used for mosquito vector control. In terms of ecological safety, however, symbiotic control as a novel approach has been proposed for mosquito control. Since there are multiple symbiotic microflora inhabiting in a variety of tissues of mosquitoes, including the digestive tract, they may affect the transmission of mosquito-borne infectious diseases through affecting the lifespan, reproductive competence, and vector competence of the host. In this review, the interactions between symbionts in mosquitoes were summarized, and the research progress of mosquito-associated symbionts in the management of mosquitoborne infectious diseases was reviewed.

Neutrophil granulocytes: participation in homeostatic and reparative processes. Part II

A supportive homeostatic function of neutrophilic granulocytes is accomplished in the physiology of diverse tissues and body systems. Neutrophils are found along the entire female reproductive tract (FRT), gradually declining in numbers from the upper parts towards the vagina. At the same time, both quantity and activity of FRT mucosal neutrophils are controlled by hormonal changes at different phases of menstrual cycle. Tissue neutrophils serve as an important source of broad-spectrum proteolytic enzymes such as matrix metalloproteinases and elastase necessary for extracellular matrix remodeling as well as vascular endothelial growth factor (VEGF) required for physiological FRT angiogenesis. During pregnancy, decidual neutrophils play a prominent role in vascular remodeling in pregnant uterus as well as development of maternal-fetal immune tolerance. The influx of neutrophils into the intestinal mucosa due to its trauma or infection not only ensures defense against pathogens, but also leads to increased proliferation of intestinal epithelial cells. Neutrophilic granulocytes elicit signals and events protective for the epithelium by marking them with a “hypoxic signature” to trigger transcription of the gene set responsible for production of mucins, mucin-modifying peptides, antimicrobial proteins, в-defensins, ultimately contributing to lesion healing and recovery of epithelial barrier function. “Inflammatory hypoxia” initiated by neutrophils and subsequent stabilization of the transcription factor hypoxia-induced factor (HIF) in intestinal epithelial cells trigger mechanisms of self-limited and resolved inflammation, which prevent excessive accumulation of neutrophils in the intestinal lumen and development of chronic inflammatory process. Neutrophilic granulocytes dominate in the oral cavity mucosa and comprise more than 95% of total leukocyte population recruited into the gingival sulcus and gingival fluid. Neutrophils maintain physiological amount and stability of symbiotic microflora composition in dental and gingival biofilms, counteracting pathogenic bacteria via phagocytosis, degranulation and extracellular trap formation, thereby ensuring healthy state in periodontal structures. Finally, similar to some other congenital disorders affecting neutrophil quantity and functions it was shown that in case of leukocyte adhesion deficiency type 1 (LAD-1) pathogenesis of periodontitis may not only be associated with a defect in their protective effector activity, but also with altered immunoregulatory function of tissue neutrophils.

ОСОБЕННОСТИ ПИЩЕВАРИТЕЛЬНЫХ И ОБМЕННЫХ ПРОЦЕССОВ У ОВЕЦ ПРИ ВКЛЮЧЕНИИ В РАЦИОНЫ ИСТОЧНИКА МЕТИЛИРОВАНИЯ

On model fi stula sheep, the eff ect of feeding a form of choline “protected” from the indirect eff ects of symbiotic microfl ora as a source of methylating agents on pregastric digestion, digestibility, and use of nutrients of feed in the body was studied. An increase in the amount of digested nutrients of feed, an improvement in the indicators of carbohydrate-fat and protein metabolism in the body of animals receiving choline was established, which gives grounds for its use in sheep breeding.

EXPLORING THE METABOLICALLY ACTIVE RUMEN MICROBIOTA AND ITS FIBROLYTIC POTENTIAL IN CROSSBRED CATTLE FED ON FIBROUS DIET THROUGH METATRANSCRIPTOMICS

The rumen wharves conglomerate of symbiotic fibrolytic microflora with the potential to produce repertoire of carbohydrate-active enzymes to elicit the degradation of recalcitrant plant lignocellulosic complex into simpler forms that can be utilized by the host. A metatranscriptomics approach was adopted to explore the metabolically active microbiota and the transcripts involved in the deconstruction of lignocellulose in Indian crossbred cattle rumen. The experimental animals were fed with mixed diet for a period of twenty-one days and the total RNA was isolated from the rumen digesta using a modified total RNA extraction protocol. After mRNA enrichment, the cDNA libraries were subjected to sequencing under Illumina HiSeq platform and generated about 2.89 GB of raw sequences. The contig assembly was accomplished using two different tools and the redundant sequences were removed before further processing. A total of 133,930 orfs were predicted from the assembled contigs using MetaGeneMark tool and the taxonomic affiliation of orfs were achieved using MEGAN 6.0 Community Edition. A total of 17 bacterial, 5 eukaryotic and 1 archaeal phylum were identified from rumen metatranscriptomic dataset. Phylum Bacteroidetes were acknowledged as the most abundant and metabolically active rumen bacterial populations in cattle rumen with 36,414 orfs corresponding to it, followed by Firmicutes with 6,349 orfs. Further, MEGAN annotation of metatranscriptomic data at species level revealed that Prevotella brevis and Prevotella ruminicola as the most metabolically active bacterial populations representing 4,022 and 2,725 orfs each. The functional annotation of metatranscriptomic data using COG database revealed that a large number of transcripts (~16%) were corresponding to translation, ribosomal structure and biogenesis and carbohydrate transport and metabolism (15%). dbCAN annotation of rumen metatranscriptomic data identified and classified 5,267 transcripts belonging to 168 CAZyme families (GH-52%, GT-26%, CBM-14%, CE-6%, PL-2% and AA-0%). The microbial community analysis of the CAZyme encoding transcripts using M5NR database revealed that a significant proportion of CAZymes were contributed by genera Prevotella (37%) and Bacteroides (21%). The cattle rumen microbiome metatranscriptome analysis presented in this study facilitated the detection of large number of transcripts encoding diverse CAZymes that actively take part in the hydrolysis of plant lignocellulose complex that may be useful for improving livestock and biofuel generation.

Periodontopathogens: a new view. Systematic review. Part 2

Relevance. The modern view of periodontitis as a dysbiotic disease that occurs as a result of changes in the microbial composition of the subgingival region is considered in a systematic review.Purpose. To study a new paradigm of development of generalized periodontitis.Materials and methods. Randomized controlled trials (RCTS) were selected for the study, including cluster RCTS, controlled (non-randomized) microbiological and clinical studies of the oral microbiome in adult patients with generalized periodontitis over the past 10 years.Results. The transition from a symbiotic microflora to a dysbiotic pathogenic community triggers the host's inflammatory response, which contributes to the development of periodontal diseases. Modern ideas about periodontal pathogenic bacteria dictate new requirements for the treatment of periodontal diseases. The second part of the review examines the microbial profiles of periodontal disease in various nosological forms, the mechanisms of the immune response and approaches to the treatment of periodontal disease from the perspective of biofilm infection.Conclusions. As follows from modern literature periodontitis is to a certain extent caused by the transition from a harmonious symbiotic bacterial community to a dysbiotic one. Recent scientific studies have shown that not single microorganism is not able to cause disease but the microbial community as a whole leads to the development of pathology.

Physiotherapy delivery systems-clinical and microbiological effectiveness in the treatment of chronic periodontitis

Relevance . Currently, the symbiotic microflora of the oral cavity plays an increasing role in the occurrence of periodontitis. Frequent use of modern medications for the treatment of patients with chronic generalized periodontitis is not the most effective therapy. One of the reasons for the low effectiveness of treatment can be considered long-term use of antibacterial drugs that lead to the development of dysbiosis in the oral cavity and immune imbalance. In order to improve the quality of periodontal treatment, dentists successfully use physical therapy. It is known that the diode laser has a high therapeutic efficiency, its use leads to a rapid subsiding of acute inflammatory phenomena, stimulation of reparative (recovery) processes, improvement of tissue microcirculation, normalization of the General immune system, and increased resistance (stability) of the body. Ozone generators used for dental treatment have a beneficial effect on periodontal tissues. Therapeutic ozone has: immunostimulating, bactericidal, fungicidal, anti-inflammatory, detoxifying and other properties. Purpose . The goal is to study the antibacterial effect of physiotherapy methods with local delivery of exposure agents. Materials and methods . We examined 80 patients with chronic generalized periodontitis of moderate severity, who, depending on the physiotherapy method used in the complex treatment of periodontitis, were divided into 2 groups of 40 people each: the first group used the Picasso led laser, and the second group used the Ozonedta device with combined local exposure to darsonvalization and ozone. The microbiological composition of periodontal pockets was determined before and after physical therapy. To isolate the aerobic microflora, we used the method of sector crops according to Gold (1965) with the calculation of the number of microorganisms in the obtained material. Anaerobes were identified by PCR using the «Multident» kit. The clinical effectiveness of the studied physiotherapy methods was evaluated 12 months after two courses of treatment (once every 6 months) using index indicators that characterize the state of periodontal tissues (PMA, CPI, Green-Vermillion, PPP, PDI, and the degree of gum recession). Results . After exposure to the Picasso diode laser, the number of aerobic microorganisms sown decreases, but not significantly. While the number of aerobic microorganisms significantly decreased after exposure to the «Ozonedta» device (P ≤ 0.001). The titer of the anaerobic microflora studied after exposure to the «Picasso» diode laser on the periodontal pocket changed in the direction of reducing the content of microorganisms by an average of 2 times, and after treatment with the ozone generator «Ozone DTA Generator» in the second group decreased by an average of 4.8 times. After 12 months of maintenance therapy, all clinical indices decreased significantly in both groups, especially the PMA index in the group where the «Ozonedta» device was used. Conclusion . Physiotherapy methods performed using the Picasso diode laser and the «Ozonedta» device for the treatment of periodontitis allow to obtain an antibacterial effect by non-drug local delivery of exposure agents in patients with chronic generalized periodontitis and significantly reduce the signs of inflammation in the periodontal tissues.

Scientific and practical aspects of the use of prebiotics in the process of feeding ruminants

The review article presents current literature data on the classification of prebiotics. Information on the characteristics of the most common and used in the feeding of ruminants prebiotic drugs is presented. Based on literature, attention is drawn to the fact that one of the main functions of mannan oligosaccharides is their competitive binding to gram-negative bacteria of the pancreas and cecum in ruminants and inhibition of pathogenic microbiota growth in these departments of gastrointestinal tract. Fructooligosaccharides are not digested by animals, starting from the oral cavity and ending with the intestines. They are readily available substrates for the microflora of the ruminant pancreas and large intestine of animals. As for galactosyloligosaccharides, experiments have convincingly proven that they stimulate the growth and development of bifidobacteria and lactobacilli, enterobacteria and streptococci in the digestive tract of animals. Lactulose and lactiol as prebiotics have a positive effect on feed intake in young animals, changing the microbial balance and biochemical composition of the contents of the cecum. These prebiotics promote the reproduction of gram-positive bacteria in the digestive tract of animals and inhibit the growth and development of clostridia. Lactulose and lactiol activate the formation of short-chain fatty acids by the microbiota of the cecum of animals, as well as increase the permeability of the intestinal mucosa and the solubility of minerals in the colon. High molecular weight beta-glucans enhance the phagocytic, cytotoxic and antimicrobial activity of macrophages. They help to produce reactive intermediates of oxygen and nitrogen and clean the tissues of apoptotic cells. Furthermore, stimulating innate immune responses, beta-glucans increase the production of anti-inflammatory cytokines and chemokines. Inulin in ruminants has a pronounced activating effect on the immune system, as well as activates the production of short-chain fatty acids in the pancreas and large intestine. The biological mechanism of action in the digestive tract of ruminants of such prebiotics as: mannan oligosaccharides, fructooligosaccharides, galactooligosaccharides, lactulose, lactiol, beta-glucans, inulin is described. The productive effect of prebiotics when using their additives in the diets of young and adult ruminants is characterized. It is shown that the use of the above prebiotic drugs in the feeding of ruminant species selectively stimulates the metabolic processes of the symbiotic microflora of the pancreas and cecum, activates their vital functions and growth. The use of prebiotic supplements in the diets of cows stimulates milk productivity and improves milk quality. In fattening cattle, the stabilization of the pH of the scar content through the use of prebiotics increases the average daily gain and live weight of animals and the efficiency of assimilation of nutrients in feed.

The estimation of streptomycin-nonspecific effect on Baikal endemic amphipods Eulimnogammarus verrucosus

In this study, we demonstrated the estimation of model antibiotic on Baikal endemic amphipods. The results showed the influence of streptomycin on crustaceans symbiotic microflora and season-dependent stress-reaction connected with glutathione S-transferase activity change.

Changes in calf productivity and resistance as a result of using the lactulose-based feed additive

Objective: the aim of the research is to make a comprehensive assessment of the prebiotic feed additive effect on calves. The experiment was carried out in work conditions on 10 black-and-white Holstein-cross calves at the age of 2 to 32 days. To conduct the experiment, an experimental group and a test group have been formed. Each group has included five milk-fed calves at the age of 2 days and older. All the calves have been given colostrum in their first 2–4 hours after birth and then they have been fed three times a day, at regular intervals. The following methods are used: clinical, microbiological, immunological and statistical. The article describes the prebiotic lactulose-based additive effect on the intestinal microbiocenosis development in one-month old calves and presents an assessment of humoral and cellular components of natural resistance in calves. The research results show a positive effect of the lactulose-based additive on the symbiotic microflora of the gastrointestinal tract that improves the natural resistance of the body and the physiological status of animals, reduces the disease duration and contributes to an increase in weight gain. After feeding the calves with the lactulose-containing preparation during their first month of life, the weight gain of each calf in the experimental group has been 21.8 kg, or + 51 % of the initial weight and in the test group 19.0 kg, or + 41 % of the initial weight. Feeding the additive has an impact on the two components of natural resistance: serum bactericidal activity in the experimental group calves has been higher by 17.8 % and the phagocytosis activity has been higher by 30.5%, compared to the test group calves. Feeding calves with the lactulose-containing additive helps reduce the illness duration, stimulates the increase in live weight and affects the natural resistance level of newborn animals positively.

Probiotics and animal feed in primates feeding

This paper presents the results of experiments on primates feeding. In diet structures that were used animal feed, of which fish flour is the most valuable, as found, for monkeys, due to its unique chemical composition. And the effect of the probiotic complex “Bactistatin” on the digestibility of feed in the diet of rhesus monkeys was studied. This preparation belongs to the new generation of probiotics. It is a biological product with immobilized probiotic strains and their metabolites. The study was conducted on a large population of male rhesus macaques in enclosure conditions. Feeding diets, their nutritional value as a result of the inclusion of fish flour in the amount of 18% were studied. And also, the effect of “Bactistatin” in the amount of 3 g/head, on the formation of symbiotic microflora of the gastrointestinal tract, was studied in order to improve the absorption of nutrients in complete feed. Changes in homeostasis during the experiment were controlled by hematological and biochemical parameters of the blood of both experimental and control primates. At the end of the experiment, the cost of feed, nutrients, and exchange energy per head was calculated. The experimental data obtained were analyzed and mathematically processed.