Sycamore Seeds Research Articles

Redox dynamics in seeds of Acer spp: unraveling adaptation strategies of different seed categories.

Seeds of woody plant species, such as those in the Acer genus like Norway maple (Acer platanoides L.) and sycamore (Acer pseudoplatanus L.), exhibit unique physiological traits and responses to environmental stress. Thioredoxins (Trxs) play a central role in the redox regulation of cells, interacting with other redox-active proteins such as peroxiredoxins (Prxs), and contributing to plant growth, development, and responses to biotic and abiotic stresses. However, there is limited understanding of potential variations in this system between seeds categorized as recalcitrant and orthodox, which could provide insights into adaptive strategies. Using proteomic analysis and DDA methods we investigated the Trx-h1 target proteins in seed axes. We complemented the results of the proteomic analysis with gene expression analysis of the Trx-h1, 1-Cys-Prx, and TrxR NTRA genes in the embryonic axes of maturing, mature, and stored seeds from two Acer species. The expression of Trx-h1 and TrxR NTRA throughout seed maturation in both species was low. The expression of 1-Cys-Prx remained relatively stable throughout seed maturation. In stored seeds, the expression levels were minimal, with slightly higher levels in sycamore seeds, which may confirm that recalcitrant seeds remain metabolically active during storage. A library of 289 proteins interacting with Trx-h1 was constructed, comprising 68 from Norway maple and 221 from sycamore, with distinct profiles in each seed category. Recalcitrant seed axes displayed a wide array of metabolic, stress response, and signaling proteins, suggesting sustained metabolic activity during storage and the need to address oxidative stress. Conversely, the orthodox seed axes presented a protein profile, reflecting efficient metabolic shutdown, which contributes to their extended viability. The results of the study provide new insights into seed viability and storage longevity mechanisms. They enhance the understanding of seed biology and lay the foundation for further evolutionary research on seeds of different categories.

Sectional Leading Edge Vortex Lift and Drag Coefficients of Autorotating Samaras

Autorotating samaras such as Sycamore seeds are capable of descending at exceptionally slow speeds and the secret behind this characteristic is attributed to a flow mechanism known as the leading edge vortex (LEV). A stable LEV is known to increase the maximum lift coefficient attainable at high angles of attack and recent studies of revolving and flapping wings have proposed suitable lift and drag coefficient models to characterise the aerodynamic forces of the LEV. For the samara, however, little has been explored to properly test the suitability of these low-order lift and drag coefficient models in describing the aerodynamic forces produced by the samara. Thus, in this paper, we aim to analyse the use of two proposed aerodynamic models, namely, the normal force and Polhamus models, in describing the sectional aerodynamic lift of a samara that is producing a LEV. Additionally, we aim to quantify the aerodynamic parameters that can describe the lift and drag of the samara for a range of wind speed conditions. To achieve this, the study first examined the samara flight data available in the literature, and from it, the profiles of the lift coefficient curves were investigated. Subsequently, a numerical Blade Element-Momentum model (BEM) of the autorotating samara encompassing different lift profiles was developed and validated against a comprehensive set of samara flight data, which were measured from wind tunnel experiments conducted at the University of Bristol for three different Sycamores. The results indicated that both the normal force and Polhamus lift models combined with the normal force drag can be used to describe the two-dimensional lift characteristics of a samara exhibiting an LEV. However, the normal force model appeared to be more suitable, since the Polhamus relied on many assumptions. The results also revealed that the aerodynamic force parameters can vary with windspeed and with the samara wing characteristics, as well as along the span of the samara wing. Values of the lift curve slope, zero-lift drag coefficient, and maximum lift coefficient are predicted and presented for different samaras. The study also showed that the low-order BEM model was able to generate a good agreement with the experimental measurements in the prediction of both rotational speed and thrust. Such a validated BEM model can be used for the initial design of bio-inspired rotors for micro-air vehicles.

Big Tree by Brian Selznick

Reviewed by: Big Tree by Brian Selznick April Spisak Selznick, Brian Big Tree; written and illus. by Brian Selznick. Scholastic, 2023 [528p] Trade ed. ISBN 9781338180633 $32.99 Reviewed from digital galleys R* Gr. 5-7 Louise and her brother, Merwin, never expected to be apart from their mom so young—they are still quite small Sycamore seeds when a fire leaves them alone in a giant and dangerous world. This world is full of terrors even if you aren’t a little seed, but for them water and wind can be risky, not to mention the dinosaurs and volcanoes. Louise is optimistic, trusting that her vibes will get her where she is destined to grow. Merwin is far more skeptical and fearful, but their love and mutual respect for each other lasts through separations, harrowing adventures, and, ultimately, two very different timelines for planting roots and becoming trees. The [End Page 232] story is unrushed, moving at a measured pace that encourages contemplation and patience: nature can be quick and wild, but trees will not be hurried. Selznick’s dedication to dual storytelling through text and hundreds of finely detailed pencil drawings allows for each to progress the plot in succession, creating a balanced and rich book that invites careful reading and poring over illustrations to find all of the connective threads. An author’s note describes how the pandemic derailed this Steven Spielberg idea from becoming a movie and how Selznick then adapted their project into a book. His expressive art that highlights the heart of the story, paired with his keen eye for creating sympathetic and memorable lost children (here he subbed in seeds for the kids) are a powerful combination to bring to this epic quest. Selznick fans will be delighted, and dinosaur fans and nature buffs will likely be just as enthralled. Copyright © 2023 The Board of Trustees of the University of Illinois

Native seed dispersal by rodents is negatively influenced by an invasive shrub

Refuge–mediated apparent competition is the mechanism by which invasive plants increase pressure on native plants by providing refuge for generalist consumers. In the UK, the invasive Rhododendron ponticum does not provide food for generalist seed consumers like rodents, but evergreen canopy provides refuge from rodent predators, and predation and pilferage risk are key factors affecting rodent foraging and caching behaviour. Here we used a seed removal/ seed fate experiment to understand how invasion by an evergreen shrub can alter seed dispersal, seed fate and early recruitment of native trees. We used seeds of four species, small and wind–dispersed (sycamore maple Acer pseudoplatanus and European ash Fraxinus excelsior) and large and animal–dispersed (pedunculate oak Quercus robur and common hazel Corylus avellana), and monitored seed predation and caching in open woodland, edge habitats, and under Rhododendron. In the open woodland, wind–dispersed seeds had a higher probability of being eaten in situ than cached seeds, while the opposite occurred with animal–dispersed seeds. The latter were removed from the open woodland and edge habitats and cached under Rhododendron. This pattern was expected if predation risk was the main factor influencing the decision to eat or to cach a seed. Enhanced dispersal towards Rhododendron cover did not increase the prospects for seed survival, as density of hazel and oak saplings under its cover was close to zero as compared to open woodland, possibly due to increased cache pilferage or low seedling survival under dense shade, or both. Enhanced seed predation of ash and sycamore seeds close to Rhododendron cover also decreased recruitment of these trees. Rhododendron patches biased rodent foraging behaviour towards the negative (net predation) side of the conditional rodent / tree interaction. This effect will potentially impact native woodland regeneration and further facilitate Rhododendron spread due to refuge–mediated apparent competition.

Nicotinamide adenine dinucleotides are associated with distinct redox control of germination in Acer seeds with contrasting physiology.

Seed germination is a complex process enabling plant reproduction. Germination was found to be regulated at the proteome, metabolome and hormonal levels as well as via discrete post-translational modification of proteins including phosphorylation and carbonylation. Redox balance is also involved but less studied. Acer seeds displaying orthodox and recalcitrant characteristics were investigated to determine the levels of redox couples of nicotinamide adenine dinucleotide (NAD) phosphate (NADP) and integrated with the levels of ascorbate and glutathione. NAD and NADP concentrations were higher in Norway maple seeds and exceptionally high at the germinated stage, being the most contrasting parameter between germinating Acer seeds. In contrast, NAD(P)H/NAD(P)+ ratios were higher in sycamore seeds, thus exhibiting higher reducing power. Despite distinct concentrations of ascorbate and glutathione, both seed types attained in embryonic axes and cotyledons had similar ratios of reduced/oxidized forms of ascorbate and half-cell reduction potential of glutathione at the germinated stage. Both species accomplished germination displaying different strategies to modulate redox status. Sycamore produced higher amounts of ascorbate and maintained pyridine nucleotides in reduced forms. Interestingly, lower NAD(P) concentrations limited the regeneration of ascorbate and glutathione but dynamically drove metabolic reactions, particularly in this species, and contributed to faster germination. We suggest that NAD(P) is an important player in regulating redox status during germination in a distinct manner in Norway maple and sycamore seeds.

Integration of MsrB1 and MsrB2 in the Redox Network during the Development of Orthodox and Recalcitrant Acer Seeds.

Two related tree species, Norway maple (Acer platanoides L.) and sycamore (Acer pseudoplatanus L.), produce desiccation-tolerant (orthodox) and desiccation-sensitive (recalcitrant) seeds, respectively. We compared the seeds of these two species to characterize the developmentally driven changes in the levels of peptide-bound methionine sulfoxide (MetO) and the abundance of methionine sulfoxide reductases (Msrs) B1 and B2, with respect to the cellular redox environment. Protein oxidation at the Met level was dynamic only in Norway maple seeds, and the reduced MsrB2 form was detected only in this species. Cell redox status, characterized by the levels of reduced and oxidized ascorbate, glutathione, and nicotinamide adenine dinucleotide (NAD)/phosphate (NADP), was clearly more reduced in the Norway maple seeds than in the sycamore seeds. Clear correlations between MetO levels, changes in water content and redox status were reported in orthodox Acer seeds. The abundance of Msrs was correlated in both species with redox determinants, mainly ascorbate and glutathione. Our data suggest that MsrB2 is associated with the acquisition of desiccation tolerance and that ascorbate might be involved in the redox pathway enabling the regeneration of Msr via intermediates that are not known yet.

Peptide-Bound Methionine Sulfoxide (MetO) Levels and MsrB2 Abundance Are Differentially Regulated during the Desiccation Phase in Contrasted Acer Seeds.

Norway maple and sycamore produce desiccation-tolerant (orthodox) and desiccation-sensitive (recalcitrant) seeds, respectively. Drying affects reduction and oxidation (redox) status in seeds. Oxidation of methionine to methionine sulfoxide (MetO) and reduction via methionine sulfoxide reductases (Msrs) have never been investigated in relation to seed desiccation tolerance. MetO levels and the abundance of Msrs were investigated in relation to levels of reactive oxygen species (ROS) such as hydrogen peroxide, superoxide anion radical and hydroxyl radical (•OH), and the levels of ascorbate and glutathione redox couples in gradually dried seeds. Peptide-bound MetO levels were positively correlated with ROS concentrations in the orthodox seeds. In particular, •OH affected MetO levels as well as the abundance of MsrB2 solely in the embryonic axes of Norway maple seeds. In this species, MsrB2 was present in oxidized and reduced forms, and the latter was favored by reduced glutathione and ascorbic acid. In contrast, sycamore seeds accumulated higher ROS levels. Additionally, MsrB2 was oxidized in sycamore throughout dehydration. In this context, the three elements •OH level, MetO content and MsrB2 abundance, linked together uniquely to Norway maple seeds, might be considered important players of the redox network associated with desiccation tolerance.

NAD(P)-Driven Redox Status Contributes to Desiccation Tolerance in Acer seeds.

Desiccation tolerance is a developmental program enabling seed survival in a dry state and is common in seeds categorized as orthodox. We focused on NAD and its phosphorylated form (NADP) because their continual switching between reduced (NAD(P)H) and oxidized (NAD(P)+) forms is involved in the modulation of redox signaling and the determination of the reducing power and further antioxidant responses. Norway maple and sycamore seeds representing the orthodox and recalcitrant categories, respectively, were used as models in a comparison of responses to water loss. The process of desiccation up to 10% water content (WC) was monitored in Norway maple seeds, while dehydration up to 30% WC was monitored in desiccation-sensitive sycamore seeds. Norway maple and sycamore seeds, particularly their embryonic axes, exhibited a distinct redox status during dehydration and desiccation. High NADPH levels, NAD+ accumulation, low and stable NAD(P)H/NAD(P)+ ratios expressed as reducing power and high NADPH-dependent enzyme activity were reported in Norway maple seeds and were considered attributes of orthodox-type seeds. The contrasting results of sycamore seeds contributed to their low antioxidant capacity and high sensitivity to desiccation. NADPH deficiency, low NADPH-dependent enzyme activity and lack of NAD+ accumulation were primary features of sycamore seeds, with implications for their NAD(P)H/NAD(P)+ ratios and reducing power and with effects on many seed traits. Thus, we propose that the distinct levels of pyridine nucleotides and their redox status contribute to orthodox and recalcitrant phenotype differentiation in seeds by affecting cellular redox signaling, metabolism and the antioxidant system.

Autumn poisoning hazards

The main poisoning hazard of autumn is the abundance of fruits and seeds following summer growth, but if the summer has seen extreme weather conditions, then pasture may be poor and horses may eat plants they would normally avoid. Usually the most significant hazard for horses in the autumn is atypical myopathy from repeated ingestion of sycamore seeds. Dried or wilted leaves of a related species, red maple, can cause haemolysis and methaemoglobinaemia in equines. Other potential risks in the autumn include yew; autumn crocus; oak (acorns); horse chestnut (conkers); plants containing cyanogenic glycosides, such as Prunus species; and ragwort. The fruit bodies of fungi (that is, mushrooms) are also abundant in warm, wet weather and although rarely reported (and possibly underreported), mushroom poisoning is a potential risk in horses. Depending on the type of mushroom there is risk of gastrointestinal, neurological, renal and liver effects. Treatment of plant and mushroom poisoning in horses will depend on the clinical signs and the plant or mushroom involved, but should include identification and removal of the plant or mushroom, and symptomatic care.

The Occurrence of Peroxiredoxins and Changes in Redox State in Acer platanoides and Acer pseudoplatanus During Seed Development

Norway maple (Acer platanoides L.) and sycamore (A. pseudoplatanus L.) are genetically closely related species that produce desiccation-tolerant (orthodox) and desiccation-sensitive (recalcitrant) seeds, respectively. Norway maple and sycamore seeds were analyzed during their development from the 14th to 24th weeks after flowering (WAF) and 11th to 21st WAF, respectively, to explore redox-related biochemical properties related to their contrasting physiology. Selected similar stages of seed development were characterized during the course of gradual decreasing water content in both seed types. The levels of protein and non-protein thiols peaked at the 18th WAF in Norway maple embryonic axes, whereas these levels constantly increased in maturing sycamore seeds. The glutathione half-cell reduction potential revealed that the cell environment adopted a more oxidized state in sycamore seeds. Peroxiredoxins (Prxs), including cytosolic/nuclear 1-Cys-Prx, cytosolic PrxIIC, mitochondrial PrxIIE, and plastidic PrxIIF, 2-Cys-Prx, and PrxQ, were detected in both species, but Norway maple embryonic axes contained higher levels of PrxIIC and PrxIIE, two Prxs with the highest peroxide detoxification potential in Arabidopsis. Redox proteomics revealed that 2-Cys-Prx was present in reduced form in both species, whereas 1-Cys-Prx was reduced uniquely in Norway maple seeds. Several enzymes, including glucose and ribitol dehydrogenase as well as fructose-bisphosphate aldolase, were oxidation-sensitive at all developmental stages in sycamore embryonic axes. Redox signaling as manifested by reactive oxygen species signals, and the oxidation of protein thiols to disulfides are discussed with respect to their significance in determining orthodox or recalcitrant seed characteristics.

Equine atypical myopathy: A metabolic study

Atypical myopathy (AM) is a potentially fatal disease of grazing horses. It is reportedly caused by the ingestion of sycamore seeds containing toxic hypoglycin A. In order to study metabolic changes, serum and urine samples from nine horses with atypical myopathy and 12 control samples from clinically healthy horses were collected and then analysed using a high-performance liquid chromatography coupled with tandem mass spectrometry; serum metabolic profiles as the disease progressed were also studied. Metabolic data were evaluated using unsupervised and supervised multivariate analyses.Significant differences were demonstrated in the concentrations of various glycine conjugates and acylcarnitines (C2–C26). Moreover, the concentrations of purine and pyrimidine metabolites, vitamins and their degradation products (riboflavin, trigonelline, pyridoxate, pantothenate), and selected organic and amino acids (aspartate, leucine, 2-oxoglutarate, etc.) were altered in horses with AM. These results represent a global view of altered metabolism in horses with atypical myopathy.

Analysis of the embryo proteome of sycamore (Acer pseudoplatanus L.) seeds reveals a distinct class of proteins regulating dormancy release

Acer pseudoplatanus seeds are characterized by a deep physiological embryo dormancy that requires a few weeks of cold stratification in order to promote germination. Understanding the function of proteins and their related metabolic pathways, in conjunction with the plant hormones implicated in the breaking of seed dormancy, would expand our knowledge pertaining to this process. In this study, a proteomic approach was used to analyze the changes occurring in seeds in response to cold stratification, which leads to dormancy release. In addition, the involvement of abscisic (ABA) and gibberellic acids (GA) was also examined. Fifty-three proteins showing significant changes were identified by mass spectrometry. An effect of ABA on protein variation was observed at the beginning of stratification, while the influence of GA on protein abundance was observed during the middle phase of stratification. The majority of proteins associated with dormancy breaking in the presence of only water, and also ABA or GA, were classified as being involved in metabolism and genetic information processing. For metabolic-related proteins, the effect of ABA on protein abundance was stimulatory for half of the proteins and inhibitory for half of the proteins. On the other hand, the effect on genetic information processing related proteins was stimulatory. GA was found to upregulate both metabolic-related and genetic information processing-related proteins. While seed dormancy breaking depends on proteins involved in a variety of processes, proteins associated with methionine metabolism (adenosine kinase, methionine synthase) and glycine-rich RNA binding proteins appear to be of particular importance.

Samaras and seedlings of Acer pseudoplatanus are potential sources of hypoglycin A intoxication in atypical myopathy without necessarily inducing clinical signs.

Ingestion of sycamore seeds (Acer pseudoplatanus) is the likely source of hypoglycin A in atypical myopathy (AM) but ingestion of seedlings in spring might also contribute to intoxication. To test for hypoglycin A in seeds and seedlings collected on pastures where AM cases were reported and compare its concentration in serum of affected and healthy horses. Field investigation of clinical cases. Whenever present, samaras (the winged nuts that each contain one seed) and/or seedlings were collected from pastures of 8 AM cases and 5 unaffected horses from different premises. Two AM cases were each co-grazing with an apparently healthy horse. Acylcarnitines and hypoglycin A were quantified in blood samples of all horses involved in the study. Hypoglycin A was detected in serum of AM (5.47 ± 1.60 μmol/l) but not in healthy controls pasturing where A. pseudoplatanus trees were not present. However, hypoglycin A was detected at high concentrations (7.98 μmol/l) in serum of a clinically healthy horse grazing a pasture with seedlings and samaras and also in the 2 healthy horses co-grazing with AM cases (0.43 ± 0.59 μmol/l). Hypoglycin A was detected in all samples of seeds and spring seedlings of A. pseudoplatanus. Atypical myopathy can be associated with the ingestion of sycamore samaras and also ingestion of seedlings. Hypoglycin A can be detected in the blood of horses with no detectable clinical signs at pasture in which there is A. pseudoplatanus. Determination of hypoglycin A concentration in blood is useful for screening for exposure in suspected cases of AM.

Influence of sycamore seed stratification length on their germination capacity

Sycamore (Acer pseudoplatanus L.) seeds are frequently used in forest nurseries and therefore the knowledge of their proper treatment is desirable. Seed stratification recommended by the Czech standard is two months but there are not many studies which review the appropriate length in Central European conditions. The paper evaluates the influence of seed stratification length on their germination capacity. The seed samples were treated in different ways to evaluate their reaction to these different handling conditions. The data proved a significant increase of germination after 42 days of stratification. The data also suggests that the better stratification medium is a substrate than wet filter paper.    

The effect of desiccation on viability and membrane lipid composition of Acer pseudoplatanus seeds

The viability of desiccation-intolerant sycamore (<em>Acer pseudoplatanus</em> L.) seeds during desiccation was investigated by tetrazolium and by a germinability test, together with membrane permeability and membrane phospholipid composition. Loss of viability was associated with an increase of solute leakage, reduced content of all phospholipid groups, decrease of unsaturated fatty acids and the unsaturated/saturated fatty acids ratio. Growth of malondialdehyde content was also observed. Some results were compared with those for tolerant to desiccation Norway maple (<em>Acer platanoides</em> L.) seeds. The results indicate active participation of membranes in the desiccation process in tolerant seeds and their decomposition in intolerant ones. The destruction of membranes was the result of lipid peroxidation, probably due to the free radical effect.

Nutritional and Phytochemical Composition of Utu (Icacina Senegalensis) and Sycamore (Ficus Sycomorus). Seeds

The nutritional and phytochemical composition of Utu (Icacina senegalensis) and sycamore (Ficus sycomorus) were studied using standard analytical techniques. The results revealed that the moisture content of sycamore (9.65 ± 0.10%) is lower than 12.89 ± 0.26% of Utu seeds respectively. therefore, sycamore will have more storage advantage than the Utu seeds. The crude fat value of both seeds are 28.62% and 31.34% for Utu and sycamore respectively. This means that both seeds can serve as energy supplier in food. The mineral composition of both seeds were analyzed. The results obtained revealed that copper, sodium and zinc have the least value of minerals in the seeds. Phosphorus, magnesium and calcium content of sycamore seeds are 380.24 ± 0.031, 300.67 ± 0.021, 390.77 ± 0.012mg/100g respectively, they are higher than the results of the utu which are 119.14 ± 0.040, 138.15 ± 0.040, 309.71 ± 0.023% mg/100g. Phytochemical screening of the two samples show small amount of antinutrient like saponin, phytate and flavonoid etc. The tannin content of Utu is 5.84 ± 0.012% which is higher than 4.03 ± 0.015% of Sycamore. The alkaloid content is higher in sycamore (5.65 ± 0.021%) than that of utu (3.92 ± 0.025%). These values are below the toxic levels, which means that they will not be harmful when consumed.

The involvement of the mitochondrial peroxiredoxin PRXIIF in defining physiological differences between orthodox and recalcitrant seeds of two Acer species.

Norway maple (Acer platanoides L., orthodox) and sycamore (Acer pseudoplatanus L., recalcitrant) belong to the same genus and grow under similar climatic conditions, but their seeds differ in their tolerance to desiccation. The initial water content (WC) of the seeds used in this study was 50%, and they were dried to 40, 20 and 7%. The mitochondrial peroxiredoxin IIF (PRXIIF) was identified in seeds of both species by immunoblotting. Semiquantitative RT-PCR analyses indicated that the transcript level of PRXIIF in both types of seeds increased during different stages of desiccation and was higher in seeds of Norway maple than in sycamore. General proteome analyses showed important differences between orthodox and recalcitrant seeds. In sycamore seeds that had been desiccated to a 7% WC, the number of protein spots and the levels of those spots were lower than in desiccation-tolerant Norway maple seeds. Post-translational modifications of PRXIIF in seeds at a 50% WC were detected via 2D electrophoresis and subsequent western blot analysis. The detected shift in the pI values (± 0.3) in A. pseudoplatanus was possibly caused by phosphorylation because several potential phosphorylation sites were predicted in silico for that protein. The gene and amino acid sequences were obtained and aligned with known sequences of other plant PRXIIF genes and proteins. High values of sequence identity were noted between the PRXIIF protein sequences of Acer species, Populus trichocarpa Torr. & A. Gray and Arabidopsis thaliana (L.) Heynh. The involvement of PRXIIF in defining the physiological differences between desiccation-tolerant and desiccation-sensitive Acer seeds is discussed in the context of its role in mitochondrial redox homeostasis.

Autumn dance ideas

Sue Sutherland and Olwyn Maurer have put together a few dance ideas based on falling leaves and spinning sycamore seeds

Ascorbate and glutathione metabolism during development and desiccation of orthodox and recalcitrant seeds of the genus Acer.

The ascorbate-glutathione system was studied during development and desiccation of seeds of two Acer species differing in desiccation tolerance: Norway maple (Acer platanoides L., orthodox) and sycamore (Acer pseudoplatanus L., recalcitrant). The results showed remarkable differences in the concentration and redox balance of ascorbate and glutathione between these two kinds of seeds during development, and a significant dependence between glutathione content and acquisition of desiccation tolerance in Norway maple seeds. There were relatively small differences between the species in the activities of enzymes of the ascorbate-glutathione cycle: ascorbate peroxidase (APX, EC 1.11.1.11), monodehydroascorbate reductase (MR, EC 1.6.5.4), dehydroascorbate reductase (DHAR, EC 1.8.5.1), and glutathione reductase (GR, EC 1.6.4.2). At the end of seed maturation, ascorbic acid content and the activities of the above enzymes was about the same in both species The electrophoretic pattern of APX isoenzymes was also similar for both species, and the intensity of the bands decreased at the end of seed maturation in both species. When sycamore seeds were desiccated to a moisture content of less than 26%, there was a marked decrease in seed viability and an increase in the production of reactive oxygen species. During desiccation, Norway maple seeds had a more active defence system, which was reflected in a higher glutathione content, a higher glutathione redox status, a higher ascorbate redox status, and higher activities of APX, MR, DHAR, GR and GPX (glutathione peroxidase). During desiccation, sulfhydryl-to-disulfide transition into proteins was more intense in Norway maple seeds than sycamore seeds. All of these results suggest that, in orthodox seeds, the ascorbate-glutathione cycle plays an important role in the acquisition of tolerance to desiccation, in protein maturation, and in protection from reactive oxygen species.

Six chemicals with animal repellent or insecticide properties are screened for phytotoxic effects on the germination and viability of ash, birch, Corsican pine and sycamore seeds

Summary As a prelude to direct sowing experiments, a laboratory study was carried out to determine whether six chemicals with insect and/or rodent repellent properties (Aaprotect, Capsaicin, Guardsman, Hallmark, Scuttle, Treeguard) affected the germination capacities and/or viability percentages of European ash (Fraxinus excelsior L.), silver birch (Betula pendula L.), Corsican pine (Pinus nigra ssp. laricio L.) and sycamore (Acer pseudoplatanus L.) seeds. None of the chemical repellents had a significant effect on either the germination capacity or viability of ash or birch seeds. One repellent (Guardsman) significantly reduced the germination capacity of sycamore from 49 to 32 per cent (P ≤ 0.01). Two repellents (Aaprotect and Scuttle) significantly reduced the germination capacity of Corsican pine from 74 to 62 per cent (P ≤ 0.05) and 11 per cent (P ≤ 0.001), respectively. Corsican pine was therefore the most sensitive of the species investigated to phytotoxic effects. Viability percentages (at the end of germination tests on repellent-treated seeds) showed that virtually all ungerminated seeds were dead. Greater phytotoxicity was observed for small-seeded species when they were incubated on a substrate of moist filter paper than when they were sown in moist peat + sand. One explanation is that the larger volume of peat + sand in the seed-tray allowed the chemicals to leach away from the seeds more quickly. Alternatively, perhaps the peat inactivated the chemicals through adsorption. The results are used to exclude certain chemical and tree seed combinations from future seed predation studies associated with direct sowing.