Mice Of Swiss-Webster Strain Research Articles

Exploring the impact of diabetes on aging: insights from TERT and COL1A1 methylation.

Aging, a multifaceted biological process, leads to diminished physical performance, especially in older adults with diabetes, where a mismatch between biological and chronological age is noticeable. Numerous studies have demonstrated that diabetes accelerates aging at the cellular and organ levels. Notable aging markers are telomerase reverse transcriptase (TERT), related to telomere length, and type 1 chain collagen (COL1A1), a key component of skin collagen. Additionally, age-related methylation increases, as revealed through methylation analysis, augmenting aspects of aging. However, the detailed interplay between aging and diabetes, particularly regarding methylation, remains underexplored and warrants further study to elucidate the biological links between the two. In this study, we elucidate the modulatory influence of diabetes on the aging process, focusing specifically on the modifications in TERT in the kidney and COL1A1 in the skin using mice of Swiss Webster strain as the diabetes model. Specimens were categorized into three distinct chronological cohorts: chronologically young (16 weeks; n = 5), chronologically old (40 weeks; n = 5), and a periodically assessed group (16 weeks; n = 30), from which five mice were systematically sacrificed on a weekly basis. Our findings reveal a marked impact of diabetes on the methylation statuses of TERT and COL1A1, characterized by an elevation in methylation levels within the periodic group (1st-6th week) and a simultaneous, progressive attenuation in the expression of TERT and COL1A1 genes. The observed alterations in the methylation levels of TERT and COL1A1 propound the hypothesis that diabetes potentially expedites the aging process, concomitantly impinging on the production of TERT and COL1A, ostensibly through the mechanism of promoter gene hypermethylation.

PENGARUH PEMBERIAN EKSTRAK ETANOL KULIT BUAH MANGGIS (Garcinia mangostana, L.) TERHADAP PENURUNAN KADAR LOGAM MERKURI PADA GINJAL MENCIT GALUR SWISS WEBSTER

<p><em>Mangosteen (Garcinia mangostana L.) is known as a plant rich in antioxidants and has various pharmacological activities. This study aims to determine the activity of the ethanolic extract of G. mangostana L. to decrease mercury levels in the kidneys. Mangosteen rind was extracted using 96% ethanol using the maceration method to form a thick extract. The experimental animals used in this study were Swiss Webster strain mice which were grouped into normal group (CMC suspension 0.5%), negative control (induction of HgCl2 solution), positive control (induction of HgCl2 solution followed by administration of curcumin at a dose of 100mg/KgBW). , and the test group was given mangosteen peel extract at a dose of 200mg/kgBW, 400mg/kgBW, 600mg/kgBW. In the test group, mice were induced using HgCl2 for 14 days, followed by administration of ethanol extract of mangosteen rind for 24 days. Observations were made with kidney organ surgery and observations were made using Atomic Absorption Spectrophotometry (AAS) and analyzed using ANOVA. The test results showed mercury accumulation in the negative, positive control group, and the test doses of 200, 400 and 600mg/kgBW, respectively, were 44.3mg/kg, 7.3mg/kg, 36.46mg/kg, 35.7mg/kg. kg and 31.34mg/kgBW with an effective dose of 600mg/KgBW.</em></p>

The administration of low-dose Curcuma longa extract induces M2 polarization in peritoneal macrophage culture

Curcuma longa (turmeric) has been widely used to accelerate wound healing, but the underlying mechanism remains unclear. Wound healing consists of four phases which are correlated and overlapping, i.e., coagulation, inflammation, proliferation and remodeling. Macrophages play an important role in most phases. Macrophage polarization to M2 initiates the proliferation phase, which is characterized by the production of several crucial growth factors. Since turmeric has been known to be an herb that accelerates wound closure, we investigated whether Curcuma longa extract administration in macrophage culture induces M2 macrophage switching. An in vitro study was performed using peritoneal macrophages from Swiss Webster strain mice. Peritoneal cells were collected and cultured in a 24-well plate. After 2 hours of incubation, macrophages (adherent cells) were treated with 0.5 ppm, 1.0 ppm and 5.0 ppm of ethanolic extract of Curcuma longa and incubated for 2 days. Quantitative real time PCR was performed to quantify M2 and M1 marker gene expression. The results revealed the upregulation of M2 marker (Arginase-1) expression upon administration of 0.5 ppm of Curcuma longa extract, but not of higher doses (1.0 and 5.0 ppm). In parallel, the ratio of Arg-1/Inos was high upon administration of 0. 5ppm of extract. In conclusion, Curcuma longa extract induces in vitro M2 polarization in low-dose administration.

The Efficacy of Noni Fruit Methanol Extract (Morinda citrofolia) to Brain Derived Neurotrophic Factor (BDNF) on Male Swiss Webster Mice Induced By Immobilization Stress

Aging process is associated with declines in certain cognitive abilities such as learning and memory ability and impact on high risk of dementia, physical disability and death. Oxidative stress is believed as basic mechanism of aging process. Morinda citrofolia (Noni fruit) has long been used as a traditional plant in worldwide and was proven empirically in traditional medicine as antioxidant. This study was to determine the effect of noni fruit methanol extract on BDNF levels in white mice of Swiss Webster strain induced by immobilization stress. This research is experimental study with post test only control group design. Male Swiss Webster Mice were induced by immobilization stress and randomized into seven groups (5 mice/groups). The first group was negative control group; Group 2,3,4,5 and 6 was given treatment with varied concentrations of Morinda citrofolia (50, 100, 200, 400 and 800 mg/kgBW). Vitamin E (70 mg/kgBW), standart antioxidant was used as positive control (Group 7). Brain BDNF level of the white mice were measured by using ELISA method. Experiment result showed that treatment with Morinda citrofolia extract (50, 100, 200,400 mg/kgBW) and Vitamin E 70 mg/kg BW showed a sigificant differences in BDNF level compare with the negative control rats. There is no significant differences between 200 mg/kgBW Metanol Extract of Morinda citrofolia group and vitamin E (p = 0,301; p > 0,05). Metanol Extract of Morinda citrofolia could prevent the decrement of BDNF level due to stress.

COMBINATION OF SAMBILOTO (ANDROGRAPHIS PANICULATA (BURM.F.) NEES) EXTRACT AND SPIRULINA (ARTHROSPIRA PLATENSIS GOMONT) TO PREVENT ANEMIA IN MICE INFECTED WITH PLASMODIUM BERGHEI ANKA

Objective: Anemia in acute malaria occurs because there is massive lysis of erythrocytes as a result of an increase in parasitemia or use of drugs that may induce hemolysis. In this research, it was tested the effect of administration of a combination of sambiloto (Andrographis paniculata Nees) extract and spirulina (Arthrospira platensis Gomont). This combination is aimed to obtain antiparasite activity of sambiloto and increasing of hemoglobin (Hb) from spirulina.Methods: This research was conducted with a completely randomized design using 75 mice of Swiss Webster strain. Test groups were consisted of AP, AP+ES, AP+PS, carboxymethyl cellulose, and dihydroartemisinin-piperaquine. All mice were infected with Plasmodium berghei on the day 0. The test material extracts were given 3 days before infection (H-3) and every day for 28 days after infection. Parasitemia data were taken on day 3, 7, 10, 15, 21, and 28, while the data for erythrocyte count and Hb level were taken on day 3, 10, and 21. Data processing was conducted using one-way ANOVA, followed by post hoc test.Results: This study showed that combination of sambiloto extract and spirulina powder (AP+PS) gave a significant difference result in suppressing the percentage of parasitemia (p=0.02), increasing the erythrocyte count (p=0.03), and Hb level (p=0.01) at the 15th day, compared to the group given only sambiloto (AP).Conclusion: Combination of spirulina powder and sambiloto extract will decrease parasitemia and increase the number of erythrocyte and Hb in mice that infected by P. berghei.

Continuous exposure of three successive generations of mice to electromagnetic fields: implication on double minute frequency

Background: Epidemiological studies indicate increased risk of leukemia, lymphoma, and brain tumor among electrical workers exposed to electromagnetic field (EMF). Other investigator reported that continuous exposure of four successive generations of mice to EMF in doses of 1 kV to 5 kV caused tumor formation in offspring. The objective of this study was to evaluate the effect of continuous exposure of three successive generations of mice ( Mus musculus L ) to EMF of 3 kV, 4 kV, and 5 kV and its implication of chromosomal breakage, as detected by double minute formation. Methods: Four couples of mice of Swiss Webster strain, 3-4 months of age, and 7-40 gram of body weight were exposed to EMF at the doses of 3 kV, 4 kV, and 5 kV, and one couple served as control. Double minute formation was examined in all offspring, except one couple of each group to be exposed with the same doses of EMF to get the F2 generation, and so forth until F3 generation. Twenty metaphases of chromosomes were examined and frequencies of double minute were calculated in the three generations of all group. Results: Frequencies of double minute in F1, F2, and F3 of mice exposed to EMF of 3 kV were respectively 0.78 ± 0.08; 0.83 ± 0.09; and 0.80 ± 0.05. In the 4 kV group were 0.083 ± 0.11; 0.73 ± 0.03; and 0.96 ± 0.15, and in the 5 kV group were 0.96 ± 0.25; 0.75 ± 0.05; and 0.99 ± 0.33, whereas no double minute chromosomes were noted in control group. Frequencies of the double minute in mice exposed to EMF were significantly higher than control group. Conclusions: Continuous exposure of mice during three successive generations to EMF at doses of 3 kV, 4 kV, and 5 kV causes increased chromosomal breakage as detected as double minute chromosome formation. (Med J Indones 2011; 20:109-13) Keywords: chromosomal breakage, double minute, electromagnetic field, mice

The effect of continuous exposure to electromagnetic fïeld on four successive generations of mice

The objective of this study is to know the biobgical effects of electromagnetic field treatment on four successive generations of mice. Fourty eight male and female mice of Swiss Webster Strain, 3 months of old, and 35 - 40 g body weight, were kept in a controlled environment and fed a standard diet. Mice were divided into 6 groups of four couples each. The first group was exposed to electromagnetic field of I kV/10 cm, the second group to 2 kV/10 cm, and the third group to 3 kV/10 cm. The remaining 3 groups were served as untreated controls of the first, second, and third group, respectively. Each couple of mice was placed in a cage (26x20x11 cm) with wire metal cage tops. The cages of experimental groups with mice inside, were then put on the negative terminal plate of a pair of parallel aluminium plate electrodes. These cages were perpendicular to the positive electrode plate at a distance of I0 cm. Subsequently, the electrodes were connected to stepup transformer as an alternating current power supply. All mice belonging to experimental and untreated control groups were allowed to mate, gastate, and deliver the first up to fourth generations, During investigation, all generations of experimental groups were continuously treated to electromagnetic field, while generations of untreated control groups received no treatment to electromagnetic field, During the study, all mice were housed in a room having a temperature of 26 ᵒ C and a light - dark cycle of 12:12 hours. The results of this study showed that exposure of mice to electromagnitic field results in reduced fertility with no change in sex ratio of the offspring. Exposure to electromagnetic field, however, were effective in inducing congenital anomalies, such as micropthalmy, white eyes, short hind legs, dwarf mice, and tumors in both sexes of the offspring which caused of death after 3 - 4 months of old. A large mortality rate were found, especially in the third and fourth generations. No congenital anomalies and tumors were noted in untreated controls. ln conclusion, we suggest that several facts which found in this study were the result of changes in genetic material of the sperm or eggs during spermatogenesis or oogenesis, respectively, i.e. a mutagenic effect. This interaction between electromagnetic field and the living cell, may then cause biological effects on cells, tissues, and organs, so that finally there are consequences for the whole organisms. (Med J Indones 2002; 11: 3-10) Keywords: electromagnetic field, fertility, mortality rate, congenital anomalies, tumor, mice