Swine Manure Application Research Articles

Effects of Different Conditioners on Soil Microbial Community and Labile Organic Carbon Fractions under the Combined Application of Swine Manure and Straw in Black Soil

The return of straw and manure to agricultural fields can impact soil organic carbon (SOC) and biological properties. However, there is a lack of research on how to use swine manure, maize straw, and various conditioners together affects soil bacterial and fungal populations. This study aimed to investigate six treatments, namely, only maize straw (S00), maize straw combined with swine manure (S0Z), maize straw combined with biochar and swine manure (SCZ), maize straw combined with boron slag and swine manure (SBZ), maize straw combined with biological agent and swine manure (SJZ), and maize straw combined with bio-organic fertilizer and swine manure (SFZ). The results showed that after the two-year return, all treatments increased the SOC content in 2023, which was 12.55–26.89% higher than S00. And the SCZ treatment significantly increased the soil organic carbon (SOC), dissolved organic carbon (DOC), easily oxidizable carbon (EOC), particulate organic carbon (POC), and microbial biomass carbon (MBC) content by 26.89%, 25.44%, 56.88%, 16.08%, and 43.54%, compared to S00. A redundancy analysis (RDA) showed that the continuous application of manure, maize straw, and conditioners has a positive impact on the diversity and abundance of soil microbial communities, enhancing the accumulation of soil carbon. Furthermore, our research revealed that soil fungi exhibited higher sensitivity in soil carbon composition following the addition of manure, straw, and conditioners to agricultural fields than bacteria. In conclusion, the addition of different conditioners to the fields is beneficial to biodiversity conservation from the perspective of achieving soil carbon storage and soil protection. Our findings suggested that the combination of maize straw, biochar, and swine manure was been proven to be the most effective treatment for increasing labile organic carbon fractions and enhancing the microbial community.

Comparison of manure application methods on nutrient and metal loss to snowmelt

Manure injection is well documented to reduce rain-driven nutrient losses, but the effect on snowmelt-driven losses is less studied. This study examined the surface application versus injection of liquid swine manure on phosphorus (P), nitrate‑nitrogen (nitrate-N), zinc (Zn), manganese (Mn), iron (Fe), magnesium (Mg), and calcium (Ca) release from soils to snowmelt. Manure was applied in the fall of 2021 to four replicated plots of surface-applied or injected treatments, and boxes were installed to collect snowmelt in each plot, including four unmanured (control) plots. In the spring of 2022, the snowmelt was collected from each box for 10 days, volume was recorded, and analyzed for dissolved reactive P (DRP), nitrate-N, Zn, Mn, Fe, Mg, and Ca concentrations, and pH. The mean daily snowmelt volume and snowmelt depth ranged from 2.9 to 25.2 L, and 2.7 to 23.3 mm, respectively. The mean snowmelt DRP concentrations for manure injected, surface-applied, and control treatments were 0.66 ± 0.07, 0.89 ± 0.07, and 0.65 ± 0.07 mg L−1, respectively. The mean DRP concentration did not vary among the manure application methods for the first 5 days of snowmelt. However, DRP was significantly higher in the manure surface-applied treatment, compared to both control and manure-injected treatments on days 7 and 8, compared to the control on day 6, and compared to the manure-injected on day 9. The effect of the manure application method on nitrate-N (3.27 to 3.60 mg L−1) and metal concentrations in the snowmelt was not significant. The cumulative loads (mean across application methods and sampling days in kg ha−1) of DRP (0.56), nitrate-N (4.03), and metals [Zn (0.11), Mn (0.01), Fe (0.18), Mg (8.03), Ca (21.70)] mobilized with snowmelt flooding did not significantly vary among the manure application methods. Results suggest that DRP, nitrate, and metal loads were primarily controlled by the snowmelt volume rather than their concentrations. Therefore, land management practices that reduce the volume of snowmelt discharge could be more effective for reducing the P, nitrate-N, and metal loss to snowmelt from manured soils.

Exogenous carbon addition soil mediated phosphorus dynamics under eight years continuous input of swine manure in a wheat-rice rotation

The study investigated the prolonged impact of swine manure application on soil inorganic phosphorus (P) fractions over an 8-year continuous, randomized field trial involving rotating wheat (wet conditions) and rice (flooded conditions) crops. The trial comprised six treatments: triplicate control plots receiving only chemical fertilizers, and triplicate plots receiving chemical fertilizers and/or swine manure ranging from 150 to 1200 kg P2O5 ha−1. The continuous application of swine manure increased soil P content and availability. Initially, P primarily accumulated in the soil as Fe-P during the first four years of manure application, transitioning to Ca8-P over the following four years. The main driver of these changes in P fractions was soil total organic carbon (TOC) was identified as the main driver of these changes in P fractions, contributing 31.5% to the observed variations. TOC increased steadily throughout the trial, with a faster rising in years 1–4 compared to years 4–8. Laboratory-scale soil incubation experiments were conducted, involving the addition of glucose or cellulose as exogenous carbon sources to test their effects on soil P dynamics and mitigate environmental damage from P leaching. The addition of cellulose to soil that had received high quantities of swine manure for eight years resulted in increased the moderately labile and moderately resistant organic P fractions while decreasing the Ca8-P and Olsen-P fractions. This promoted adsorption of high-activity inorganic P to organic matter, consequently limiting the accumulation of Ca8-P. The long-term application of swine manure altered soil P fractions and enhances P lability, enhancing P lability and availability. This study identified an optimized the risk of leaching of labile P from soil under a fertilization regime applying 10,300 kg ha−1 yr−1 swine manure. To further mitigate the risk of P leaching, the inclusion of additional cellulose-based organic matter (e.g., straw) in swine manure fertilization regimes is recommended.

Effects of Mixed Application of Chemical Fertilizer and Liquid Swine Manure on Dry Matter Yield and Feed Value of Whole Crop Barley

Effects of Mixed Application of Chemical Fertilizer and Liquid Swine Manure on Dry Matter Yield and Feed Value of Whole Crop Barley

Phosphorus extractors in soil under no-tillage system with 19 years of swine manure applications

The objective of this study was to evaluate the availability of phosphorus (P) in a soil under no-tillage system after successive applications of liquid swine manure (LSM) doses in soil samples collected at different depths and to select the most appropriate chemical extractors. It was used soil with LSM applications for 19 years, using doses of 0, 25, 50, 75 and 100 m3 ha−1 and mineral fertilization (350 kg ha−1 in formulation 02-20-18), evaluated at the following depths: 0–10, 10–20 and 20–40 cm. The extractors used were Mehlich-1, Mehlich-3, Prem, Olsen, Bray-1 and Resin. Successive fertilizations with LSM, especially with 100 m3 ha−1, increase the availability of P, especially in the 0–10 cm layer, as well adding P in the deeper layers evaluated (20–40 cm). The organic P content in relation to the total P ranged from 16 to 19 %. Bray-1, Olsen and Resin extractors are more efficient in extracting P in soil under no-tillage cultivation after successive fertilizations with liquid swine manure.

Swine Manure Reduces Nitrous Oxide Emissions from Acidic Red Soil Due to Mineral N Immobilization and Alleviated Acidification

Swine manure is widely used for ameliorating red soil acidification, but little information is available about its effect on N2O emissions. To explore the effects, a 35-day incubation experiment was conducted with two soils under different fertilization history: chemical fertilizers only (F) and combination of chemical fertilizers with swine manure (M). The treatments included no fertilizer (control), 100% N from urea (M0), and urea plus swine manure, which supplied 20% (M20), 40% (M40), 60% (M60), and 100% (M100) of total N. Soil N2O emission rates, pH, exchangeable acidity, mineral N species, dissolved organic carbon and nitrogen, microbial biomass carbon, and their inner relationships were examined. The N2O emission rates markedly increased following the treatments, reached peaks before day 2, and thereafter decreased sharply to the level of the control by day 25, 25, 23, 15, and 9 in F soil and by day 25, 25, 23, 19, and 11 in M soil for M0, M20, M40, M60, and M100 treatments, respectively. As swine manure application rate increased, the cumulative N2O emissions of F soil decreased significantly, while, for M soil, there was no significant difference among M0, M20, M40, and M60 treatments, which were higher than the M100 treatment. At the end of incubation, soil pH in F and M soils followed the order M0 < M20 < M40 < M60 < control < M100 and vice versa for exchangeable Al3+ and acidity. F soil had relatively higher NH4+-N concentration in M0 treatment and higher NO3−-N concentrations in M0 and M20 treatments than M soil. Soil pH and NH4+-N had the greatest relative contribution to N2O emissions. Overall, this study indicates that partial chemical N replacement by swine manure could effectively mitigate N2O emissions from acidic red soil primarily because of mineral N immobilization and alleviated red soil acidification. Thus, swine manure has the potential to co-ameliorate red soil acidification and N2O emission. Further research is needed to determine the effect of swine manure on N2O emission reductions under field conditions and the overall benefit in effective N management.

Risk of Heavy Metal Contamination in Vegetables Fertilized with Mushroom Residues and Swine Manure

Mushroom residues and swine manure are two common types of agricultural waste that are often returned to fields as organic fertilizers. However, the environmental risks of their reclamation, such as heavy metal pollution, have been less studied. To investigate the potential risks of heavy metal contamination in soils and vegetables after continuously applying mushroom residues and swine manure, field experiments of four consecutive vegetable rotations were conducted in the Qingpu District of Shanghai, Southeast China, from 2019 to 2021. The concentrations of heavy metals in soils continuously fertilized with mushroom residues and swine manure gradually increased. The organic matter content in the soils exhibited a significant correlation with the concentrations of Cu, Zn, Pb, and Cd (p < 0.01), suggesting that the increase in heavy metals is attributed to the use of organic waste. In particular, the application of swine manure increased Cu, Zn, Pb, and Cd concentrations in the soils by 118.3%, 54.9%, 57.6%, and 122.2%, respectively. Moreover, the application of organic waste raised the risk of the bioaccumulation of toxic metals, such as Cd, in vegetables. The Cd concentration was significantly and positively correlated with Zn in the edible parts of vegetables (p < 0.05). The recycling of swine manure more significantly enhanced Cd concentrations in the edible parts of green pepper (Capsicum annuum), eggplant (Solanum melongena), Brassica chinensis, and lettuce (Lactuca sativa), which were 2.53, 1.55, 1.66, and 1.62 times that of the non-fertilizer control (CK), respectively. Although the increase in heavy metals in the soils and vegetables was still mild when compared with the set thresholds of soil and food safety after the four vegetable rotations, the trend of increase in toxic heavy metals in the food chain with a continuous application of organic waste should be carefully considered.

Differential impact of swine, bovine and poultry manure on the microbiome and resistome of agricultural grassland

Land spreading of animal manure is an essential process in agriculture. Despite the importance of grassland in global food security the potential of the grass phyllosphere as a reservoir of antimicrobial resistance (AMR) is unknown. Additionally, the comparative risk associated with different manure sources is unclear. Due to the One Health nature of AMR there is an urgent need to fully understand the risk associated with AMR at the agriculture - environmental nexus.We performed a grassland field study to assess and compare the relative and temporal impact of bovine, swine and poultry manure application on the grass phyllosphere and soil microbiome and resistome over a period of four months, using 16S rRNA amplicon sequencing and high-throughput quantitative PCR (HT-qPCR). The soil and grass phyllosphere contained a diverse range of antimicrobial resistance genes (ARGs) and mobile genetic elements (MGEs). Manure treatment was found to introduce ARGs belonging to clinically important antimicrobial classes, such as aminoglycoside and sulphonamide into grass and soil. Temporal analysis of ARGs and MGEs associated with manure treatment indicated ARGs patterns were similar across the different manure types in the manure treated soil and grass phyllosphere. Manure treatment resulted in the enrichment in members of the indigenous microbiota and the introduction of manure associated bacteria, with this impact extending past the recommended six-week exclusion period. However, these bacteria were in low relative abundance and manure treatment was not found to significantly impact the overall composition of the microbiome or resistome. This provides evidence that the current guidelines facilitate reduction of biological risk to livestock. Additionally, in soil and grass samples MGEs correlated with ARGs from clinically important antimicrobial classes, indicating the key role MGEs play in horizontal gene transfer in agricultural grassland. These results demonstrate the role of the grass phyllosphere as an under-studied sink of AMR.

Responses of soil microbial communities to manure and biochar in wheat cultivation of a rice-wheat rotation agroecosystem in East China

Soil contamination in agroecosystems remains an environmental problem of global concern. Biochar has been suggested as an organic amendment to alleviate soil pollution, sequester carbon, and improve fertility. Yet, information is still lacking on how bacterial and fungal communities in acidic bulk and rhizosphere soils respond to swine manure and its biochar. In this study, biochar and swine manure were applied at two rates of 1.5 and 3 t ha-1 in a rice-wheat rotation field study to assess how soil characteristics, especially pH and the availability of chemical elements, correlate to compositional variations of bacteria and fungi in bulk and rhizosphere soils. Our results showed that biochar and manure amendments at higher rates promoted bacterial richness index in bulk and rhizosphere soils through increasing soil pH and reducing As and Cu availability. For beta diversity, compared with As and Cu availability, soil pH had opposite effects on both bacterial and fungal communities. Specifically, biochar and swine manure application stimulated bacterial class of Gemmatimonadetes, Deltaproteobacteria, and Gammaproteobacteria via increasing pH and decreasing available chemical elements. Opposite trends of fungal community responding to biochar and manure was found. For example, biochar restrained the fungal class of Eurotiomycetes via decreasing As and Cu, but manure inhibited Leotiomycetes mainly due to an increase in soil pH and a decrease in soil dissolved organic C (DOC). These evidences suggest that both bacterial and fungal communities respond significantly to biochar and manure amendments in both bulk and rhizosphere soils, possibly due to their sensitive adaptation to soil environmental variations, such as pH levels and availability of chemical elements.

Effects of Mixed Application of Chemical Fertilizer and Liquid Swine Manure on Agronomic Characteristics, Yield and Feed Value of Rye (<i>Secale cereale</i> L.)

Effects of Mixed Application of Chemical Fertilizer and Liquid Swine Manure on Agronomic Characteristics, Yield and Feed Value of Rye (<i>Secale cereale</i> L.)

Impact of Swine and Cattle Manure Treatment on the Microbial Composition and Resistome of Soil and Drainage Water.

Evaluating potential environmental and clinical impacts of industrial antibiotic use is critical in mitigating the spread of antimicrobial resistance. Using soil columns to simulate field application of swine or cattle manure and subsequent rain events, and a targeted qPCR-based approach, we tracked resistance genes from source manures and identified important differences in antimicrobial resistance gene transport and enrichment over time in the soil and water of artificially drained cropland. The source manures had distinct microbial community and resistance gene profiles, and these differences were also reflected in the soil columns after manure application. Antibiotic resistance genes (ARGs) were only significantly enriched in effluent samples following the first rain event (day 11) for both soil types compared to the control columns, illustrating the high background level of resistance present in the control soils chosen. For swine, the genes tetQ, tet(36), tet44, tetM, sul2 and ant(6)-ib persisted in the soil columns, whereas tetO, strB and sul1 persisted in effluent samples. Conversely, for cattle manure sul2 and strB persisted in both soil and effluent. The distinct temporal dynamics of ARG distribution between soil and effluent water for each manure type can be used to inform potential mitigation strategies in the future.

Manure application: A trigger for vertical accumulation of antibiotic resistance genes in cropland soils

The application of livestock manure increases the dissemination risk of antibiotic resistance genes (ARGs) in farmland soil environment. However, the vertical migration behavior and driving factor of ARGs in manured soil under swine manure application remains undefined. Here, the dynamics of ARGs, mobile genetic elements (MGEs) and bacterial communities in different soil depths (0 – 80 cm) with long-term swine manure application were tracked and conducted using real-time qPCR. Results showed that long-term application of swine manure remarkably facilitated the vertical accumulation of ARGs and MGEs, in particular that the relative abundance of blaampC showed significant enrichment with increasing depth. ARGs abundance was similar in the three fields with long-term application of swine manure. (p＞0.05). Procrustes analysis indicated that microbial communities were the dominant drivers of ARGs variation in topsoil, and the changes of environmental factors played a vital role in vertical migration ARGs in cropland soils. Additionally, the variation patterns of high-risk ARGs (i.e., blaampC, blaTEM-1) were influenced by the dominant bacteria (Actinomycetes) and pH. This study illustrated that the swine manure application promoted the vertical migration of ARGs, including multidrug resistance determinants, highlighting the ecological risk caused by long-term manure application.

Agri-environmental implications of N- and P-based manure application to perennial and annual cropping systems

Continuous manure application based on crop nitrogen (N) requirements could substantially increase field nutrient losses from croplands. Although phosphorus-based (P) manure application is an alternative, crops may suffer from potential microbial P immobilization and fixation of P in soil. A three-year study (2012–2014) was carried out in Manitoba, Canada, to evaluate the agronomic and environmental benefits and tradeoffs between N- and P-based liquid and solid swine manure applications on previously established (2009) annual (ACS) and perennial (PCS) cropping systems. The N-based solid manure produced greater aboveground biomass and grain yields of barley than the unfertilized control in the ACS. The N-based liquid manure produced greater biomass than the control in the PCS. Phosphorus-based treatments produced statistically similar canola oilseed grains as the N-based treatments. Seeding the PCS to canola in 2013 produced greater aboveground biomass yields than the ACS; however, the canola oilseed yields were not significantly different between the two systems. The N-based solid manure application increased Olsen P by approximately 30 mg kg−1 in both cropping systems during the three-year study period. Both N- and P-based liquid manure treatments and the N-based solid manure treatment lost significantly greater nitrate through leaching than the control in 2013, when most leaching losses occurred. Our study also showed that some of the environmental benefits of the perennial cropping system, such as reduced nitrate leaching, could be lost when converted to an annual cropping system.

Long-term application of swine manure and sewage sludge differently impacts antibiotic resistance genes in soil and phyllosphere

Field application of organic fertilizers may promote the spread of antibiotic resistance genes (ARGs) in farmland ecosystems, which represents a major threat to public health. However, impacts of multi-year organic fertilization on the spread of soil and phyllosphere ARGs and the possible transmission pathways remain elusive. Here, we investigated the patterns of ARGs and structures of bacterial and fungal communities in soil and wheat crop phyllosphere, under long-term fertilization of swine manure or sewage sludge using high-throughput quantitative PCR and amplicon sequencing. It was found that application of swine manure and sewage sludge had more influence on the prevalence of ARGs in phyllosphere than in soil. SourceTracker analysis revealed that a few bacterial taxa from swine manure and sewage sludge could survive in soil, but hardly in phyllosphere. Mantel test and variation partitioning analysis further revealed that shift in the microbial community compositions was an important factor affecting soil ARGs, while horizontal gene transfer mediated by mobile genetic elements might contribute to the spread of phyllosphere ARGs. Overall, these results demonstrated that long-term application of swine manure and sewage sludge differently impacts the ARGs in soil and phyllosphere, which have implications for sustainable agricultural management.

Liquid Swine Manure Application Variability: Challenges and Opportunities

HighlightsVariability of manure N content on the day of application was a major contributor to plot-to-plot N rate variability.Using the average of previous years’ manure samples is a valid strategy to improve the accuracy of P application.Estimated economic losses from missing target manure N rates averaged $34.27 ha-1 year-1 ($13.87 ac-1 year-1).Improving N rate accuracy with real-time N sensing provided an estimated value of $7 to $44 ha-1 ($3 to $18 ac-1).Abstract. Liquid livestock manure application presents both opportunities for replacing commercial fertilizer and challenges to doing so accurately. This study demonstrated the challenges in achieving a target N application rate using liquid swine manure on eighteen 0.4 ha research plots at the Northeast Research and Demonstration Farm (NERF) near Nashua, Iowa. Differences between the nutrient analyses of manure from a pre-application sample and samples taken on the day of application, equipment adjustments, field conditions, and changing manure nutrient content during pit pumping contributed to variability in the actual N rate applied. The coefficient of variability (CV) of manure N during manure pumping ranged from 2.7 to 13.6, with an 8-year average of 6.5. A weighted-average CV for applied manure volume ranged from 1.5 to 4.7, with an 8-year average CV of 3.2, suggesting that plot-to-plot variability in manure volume was less of an issue for achieving the target N rate than was the variability of manure N on the day of application. A separate analysis of manure from six Iowa swine farms showed that using the average of the previous years’ manure samples improved accuracy for phosphorus at Farm 2, whereas using a single sample from the application year improved accuracy for potassium at Farm 5. Averaged across the six farms, using the average of the previous years’ manure samples resulted in less error for phosphorus than using a single sample from the application year. For nitrogen, there was no significant difference between the two approaches within or across farms (p < 0.1). The average economic loss from missing the target N rate in the NERF study was $34.27 ± $32.49 ha-1 year-1 ($13.87 ± $13.15 ac-1 year-1). The estimated economic value from using real-time nutrient sensing to improve N rate accuracy ranged from $7.56 to $32.03 ha-1 ($3.06 to $12.96 ac-1) in a corn-soybean rotation and from $10.38 to $44.41 ha-1 ($4.20 to $17.97 ac-1) in continuous corn. This study illustrates the need for new technologies for real-time manure nutrient sensing and on-the-go rate adjustment. The economic analysis can serve as a guide for manure applicators when evaluating the feasibility of manure nutrient-sensing systems. Keywords: Manure application, Manure management, Manure nutrient content, Manure nutrient sensing, Manure rate calibration.

Crop Production and Phosphorus Legacy with Long-Term Phosphorus- and Nitrogen-Based Swine Manure Applications under Corn-Soybean Rotation

The traditional manure management strategy, based on crop N needs, results in accumulation of phosphorus (P) in soil due to the imbalance of N/P ratio between crop requirement and manure supply. This study was conducted from 2004 to 2013 to evaluate the effects of P-based liquid and solid swine manure (LMP and SMP, for P-based liquid and solid swine manure, respectively) application, in comparison with N-based application (LMN and SMN, for N-based liquid and solid swine manure, respectively), on crop yield and soil residual P under corn (Zea mays L.)–soybean (Glycine max L.) rotation in a Brookston clay loam soil of the Lake Erie basin, ON, Canada. Chemical fertilizer P (CFP) and non-P treatments were included as controls (CK). For liquid manure treatments, corn yield for LMN showed a lower annual corn yield (7.82 Mg ha−1) than LMP (9.36 Mg ha−1), and their differences were even statistically significant at p < 0.05 in some cropping years. The annual corn yield of LMP was also higher than those of SMP (7.45 Mg ha−1) and SMN (7.41 Mg ha−1), even the CFP (8.61 Mg ha−1), although the corresponding yield differences were not significant (p < 0.05) in some cropping years. For soybean, the plots with P application produced an average of 0.98 Mg ha−1 greater annual yields than CK. No significant differences were found between CFP and manure treatments. The annual corn yield of SMN was close to that of the CK (7.19 Mg ha−1). The grain P removal (GPR) of SMN (18.6 kg ha−1) for soybean was significantly higher than that of the other treatments. The above-ground-P uptake (AGPU) in SMN, for both corn and soybean, was significantly higher than that of the other five treatments. The soil test P (STP) presented clear stratification, concentrating in the top 30 cm soil depth after 10 years of application. The contents of STP with LMN and SMN increased from 7.1 mg P kg−1 to 12.4 and 45.5 mg P kg−1, respectively. The sum of STP mass (0–30 cm) with LMP (31.6 kg ha−1) was largely identical to that with CFP (30.1 kg ha−1); however, with SMN (173.7 kg ha−1), it was significantly higher than the rest of the treatments. Manure P source availability coefficients were averaged at 1.06 and 1.07 for LMP and SMP, respectively. The addition of phosphorus-based liquid or solid swine manure can overcome the drawback of traditional N-based applications by potentially reducing the adverse impact on water quality while sustaining crop agronomic production.

Ammonia and nitrous oxide emission factors for excreta deposited by livestock and land-applied manure.

Manure application to land and deposition of urine and dung by grazing animals are major sources of ammonia (NH3 ) and nitrous oxide (N2 O) emissions. Using data on NH3 and N2 O emissions following land-applied manures and excreta deposited during grazing, emission factors (EFs) disaggregated by climate zone were developed, and the effects of mitigation strategies were evaluated. The NH3 data represent emissions from cattle and swine manures in temperate wet climates, and the N2 O data include cattle, sheep, and swine manure emissions in temperate wet/dry and tropical wet/dry climates. The NH3 EFs for broadcast cattle solid manure and slurry were 0.03 and 0.24 kg NH3 -N kg-1 total N (TN), respectively, whereas the NH3 EF of broadcast swine slurry was 0.29. Emissions from both cattle and swine slurry were reduced between 46 and 62% with low-emissions application methods. Land application of cattle and swine manure in wet climates had EFs of 0.005 and 0.011 kg N2 O-N kg-1 TN, respectively, whereas in dry climates the EF for cattle manure was 0.0031. The N2 O EFs for cattle urine and dung in wet climates were 0.0095 and 0.002 kg N2 O-N kg-1 TN, respectively, which were three times greater than for dry climates. The N2 O EFs for sheep urine and dung in wet climates were 0.0043 and 0.0005, respectively. The use of nitrification inhibitors reduced emissions in swine manure, cattle urine/dung, and sheep urine by 45-63%. These enhanced EFs can improve national inventories; however, more data from poorly represented regions (e.g., Asia, Africa, South America) are needed.

Long-term effects of inorganic fertilizers and organic manures on the structure of a paddy soil

Inorganic fertilizers and organic manures have been widely used to maintain crop yields. Leguminous green manures are usually used in the paddy fields in southern China. But the effect of green manures or combination of green manures with other fertilizers on soil physical properties, especially soil pore structure, is unclear. Based on a 34-year field experiment, we investigated the effects of different fertilization strategies on soil structural properties of a paddy soil. Four treatments were selected: no amendment as a control (CK), inorganic fertilization of N, P and K (NPK), combined application of inorganic fertilizers and green manure (GM), and combined application of inorganic fertilizers, green manure and swine manure (GSM). Results showed that relative to the CK treatment, the NPK, GM and GSM treatments increased SOC content by 7.9 %, 17.8 % and 25.7 %, respectively (P < 0.05). The increase of SOC content led to increased aggregate water stability in the GSM treatment (P < 0.05) but not in the GM and NPK treatments (P > 0.05). Consistent with SOC, soil bulk density was reduced by 5.6 %, 7.4 % and 17.6 %, respectively, for the NPK, GM and GSM treatments (P < 0.05). Total porosity was significantly increased in all the fertilized treatments (P < 0.05). Detailed pore size distribution analysis revealed that the NPK treatment mainly increased < 25 μm pores (total porosity minus porosity of pores that are > 25 μm), while the GM and GSM treatments mainly increased > 25 μm pores. The GSM treatment also significantly increased the connectivity and fractal dimension of macropores (P < 0.05). The penetration resistance of the paddy soil was not affected by the different fertilization strategies, with a low (< 0.4 MPa) value at the plough layer (0−15 cm) and a high value (up to 1.6 MPa) at the plough pan (15−25 cm). Overall, this study indicated that inorganic fertilizers had beneficial, but limited effects on paddy soil structure. Green manure and swine manure can further improve soil pore structure and soil aggregation.

Impacts caused by swine manure application and proper management proposition in a swine finishing farm

Due to the increase in the PWW production level by the high concentration of animals per area, the intensification and concentration of pig farming in certain regions has resulted in the intensive application of swine manure (PWW) in the same terrain, which may provide the soil accumulation and the transference of elements may compromise the productive capacity of the territorial space of that region. In this sense, this work aimed to evaluate the elements concentration present in the soil and in swine waste, in relation to the values stipulated by CONAMA Resolution n. 420 (Conselho Nacional do Meio Ambiente [CONAMA], 2009) and also by CETESB n. 195/2005 (Companhia de Tecnologia Ambiental do Estado de São Paulo [CETESB], 2005), which provides for criteria and values guiding soil quality and groundwater for chemicals presence, and, based on the reference element, adapt to the technical standard P 4,231/2015 (Companhia de Tecnologia Ambiental do Estado de São Paulo [CETESB], 2015) proposed by CETESB for the dose calculation of vinasse application, in PWW application in pasture cultivation. The work accomplished at Fazenda Talhado Cinco de Março rural area - Rio Verde Municipality – Goiás state. Samples were performed to determine the soil physicochemical attributes and PWW chemical analysis of the soil, submitted to statistical analysis of Analysis of Variance and Tukey’s Test 5%. The results made it possible to verify that the used management is not the most appropriate, and it is necessary to review the procedures currently adopted, such as performing more constant soil and PWW analyzes, to avoid that their continual application in a disorderly manner and with possible negative impacts on the environment.

Dissipation of antibiotic resistance genes in manure-amended agricultural soil

Soil antibiotic resistance due to animal manure application is of great concern in recent years. Little is known about the fate of antibiotic resistance genes (ARGs) in agricultural soils associated with long-term manure application. Here we used soil microcosms to investigate the dissipation of ARGs and the change of bacterial community in agricultural soil originated from a vegetable field which had received 24 years' swine manure application. Soil microcosms were conducted at different soil moistures and with or without biochar over a testing period of two years in lab. Results showed that continuous manure application induced an accumulation of ARGs in soil, wherein the dissipation of ARGs differed from those in non-manure amended soil. ARGs persisted in soils at least two years, although their abundance declined gradually. Meanwhile, soil moisture and biochar had significant impact on the fate of ARGs. ARGs dissipated faster in soil with higher moisture. Biochar amendment contributed to the maintenance of bacterial diversity. Within the two years of simulation experiment, biochar enhanced soil ARG retention as they dissipated slowly in the soil amended with biochar. Succession of microbial community may have sustained the transfer and resilience of ARGs. This study provides insight into the dissipation of antibiotic resistance genes in manure-applied agricultural soil.