Swimming Capacity Research Articles

Determining the Fish Migration Patterns Based on Fish Habitat Assessment at a River Confluence

ABSTRACTMuch effort has been devoted to predicting fish migration routes to assist target species migration, and yet, fish migration science and practice remain imperfect. This study aimed to predict the migration paths of Gymnocypris przewalskii at the river confluence in the Qinghai Lake basin. The authors proposed an approach for fish migration route prediction, which involves a hydrodynamic module, a habitat model and a fish migration module. The TELEMAC‐MASCARET system was used to simulate hydrodynamic conditions and statistical analyses were carried out. During the flood season, G. przewalskii migrates downstream, whereas during the migratory season, they migrate upstream. The results indicate that flow velocity was the most significant hydrodynamic parameter affecting fish migration behaviour. The optimal flow velocity range for the target fish species was between 0.7 and 1.7 m/s. The impact of water depth was only observed in low discharge situations. Besides, the temperature plays a vital role in determining fish migration and abundance. However, the results reveal that in the morning hours, the temperature exhibits a range of 10°C to 16°C, and in the noon, the average temperature ranges from 16°C to 19°C, with a maximum temperature reaching 23°C. These temperature variations enable fish to migrate towards the tributary offering a more favourable water temperature during route selection at a river confluence. The study concludes that future research should consider incorporating the swimming capacity of the focal fish species to provide insights into fish migration patterns.

BolA-like protein (IbaG) promotes biofilm formation and pathogenicity of Vibrio parahaemolyticus.

Vibrio parahaemolyticus is a gram-negative halophilic bacterium widespread in temperate and tropical coastal waters; it is considered to be the most frequent cause of Vibrio-associated gastroenteritis in many countries. BolA-like proteins, which reportedly affect various growth and metabolic processes including flagellar synthesis in bacteria, are widely conserved from prokaryotes to eukaryotes. However, the effects exerted by BolA-like proteins on V. parahaemolyticus remain unclear, and thus require further investigation. In this study, our purpose was to investigate the role played by BolA-like protein (IbaG) in the pathogenicity of V. parahaemolyticus. We used homologous recombination to obtain the deletion strain ΔibaG and investigated the biological role of BolA family protein IbaG in V. parahaemolyticus. Our results showed that IbaG is a bacterial transcription factor that negatively modulates swimming capacity. Furthermore, overexpressing IbaG enhanced the capabilities of V. parahaemolyticus for swarming and biofilm formation. In addition, inactivation of ibaG in V. parahaemolyticus SH112 impaired its capacity for colonizing the heart, liver, spleen, and kidneys, and reduced visceral tissue damage, thereby leading to diminished virulence, compared with the wild-type strain. Finally, RNA-sequencing revealed 53 upregulated and 71 downregulated genes in the deletion strain ΔibaG. KEGG enrichment analysis showed that the two-component system, quorum sensing, bacterial secretion system, and numerous amino acid metabolism pathways had been altered due to the inactivation of ibaG. The results of this study indicated that IbaG exerts a considerable effect on gene regulation, motility, biofilm formation, and pathogenicity of V. parahaemolyticus. To the best of our knowledge, this is the first systematic study on the role played by IbaG in V. parahaemolyticus infections. Thus, our findings may lead to a better understanding of the metabolic processes involved in bacterial infections and provide a basis for the prevention and control of such infections.

The Effects of Water Flow Speed on Swimming Capacity and Energy Metabolism in Adult Amur Grayling (Thymallus grubii)

The present study aimed to explore whether water flow velocity could affect the swimming ability and overall energy metabolism of wild Amur grayling (Thymallus grubii). Swimming performance was assessed by measuring critical swimming speed (Ucrit), burst speed (Uburst), and oxygen consumption rate (MO2) based on the stepped velocity test method. Our results showed that the absolute values of Ucrit and Uburst tended to increase with body length. In contrast, the relative values of Ucrit and Uburst tended to decrease and increase, respectively. MO2 in Amur grayling was elevated with increasing velocity, suggesting relatively high swimming efficiency. We also measured the biochemical indices related to energy metabolism. Lactate dehydrogenase, hexokinase, and pyruvate kinase activities significantly increased (p < 0.05). Hepatic glycogen, glucose, and muscle glycogen contents decreased with the increasing trend of velocity (p < 0.05), the lactic acid contents of the blood and muscles increased significantly with the increase in velocities (p < 0.05), and changes in creatine phosphate content showed no significant difference (p > 0.05). The results not only denote the relationship between body size and swimming speed but also show the effects of water flow velocity on energy metabolism in Amur grayling. The results provide basic data for the construction of fish passage.

Influence of microplastic pollution on the toxicity of potamodromous fish grass carp (Ctenopharyngodon idellus) and its swimming capacity

Microplastics (MPs), as a ubiquitous environmental contaminant in marine and freshwater systems, produced a significant influence on the physiological characteristics of aquatic organisms, and inevitably posed unknown effects on their swimming capacity. Herein, we evaluated the MPs accumulation in potamodromous fish, Ctenopharyngodon idellus, and their potential effects on oxidative stress, neurotoxicity, gut microbial diversity, and swimming capability through a toxic-control experiment. Juvenile fish were exposed to 5 µm polystyrene-MPs (PS-MPs) for 6 days at concentrations of 0.1, 1, and 10 mg/L. Exposure to PS-MPs resulted in significant inhibition of acetylcholinesterase (AChE) in the brain of fish, with significant increases in superoxide dismutase (SOD) activity and malondialdehyde (MDA) levels at 1 and 10 mg/L exposures. Meanwhile, MPs altered grass carp gut microbiota and reduced the Shannon diversity index. In addition, MPs significantly increased induced swimming speed by observing the flow rate at which grass carp happened to produce a reverse current behavioral response. The 1 and 10 mg/L of MPs treatments can significantly reduce the critical swimming speed of grass carp, meaning that the completion of the life history of grass carp may be delayed. Furthermore, there were significant correlations between biomarkers and indicators of swimming capacity. Therefore, the present study suggests that MPs produced a negative effect on the physiological properties of fish, reducing the swimming capacity of migratory fish.

Swimming Performance Assessments of Fish Species of Greatest Conservation Need to Inform Future Stream Crossing Designs in Texas

Conventional structures associated with stream crossings such as bridges and culverts can lead to zones of high-velocity water flow that impede fish passage. Such obstacles are likely to harm native fish populations by impacting migrations critical to their life history strategies, causing habitat fragmentation and extirpation, thereby limiting population growth and distribution. Due to the high rates of human population growth and development occurring in Texas, this is an issue of particular concern for fish designated as species of greatest conservation need (SGCN). This project focused on four SGCN fishes native to the Edwards Plateau Ecoregion, namely the Guadalupe Bass, Guadalupe Roundnose Minnow, Guadalupe Darter, and Plateau Shiner, at both adult (all species) and juvenile (Guadalupe Bass only) life stages. Our primary aim was to aid in the design of future culverts associated with stream crossings (or the modification of existing culverts) so that the water velocities through these structures do not exceed the swimming capacities of our target SGCN fishes. To this end, we assessed their maximum sustained swimming speeds (Ucrit) under a typical range of Edwards Plateau stream temperatures (15, 22.5, and 30 °C) to be used in site-specific calculations of the maximum allowable culvert water velocities (Vf). A secondary objective was to collect physiological endpoints of relevance to the overall swimming performance including the maximum burst swimming speeds (Umax), metabolic rate measurements (i.e., standard metabolic rate (SMR), maximum metabolic rate (MMR), and aerobic scope (AS)), cost of transport (COT), and optimal swimming speed (Uopt). Temperature-related effects were observed that can be used to inform site-specific culvert designs. In general, the highest Ucrit values for the tested species were near, or possibly between, 22.5 and 30 °C, while Umax stayed relatively consistent among treatments.

Fluid-Solid Interfacial Properties and Drag-Reducing Characterization of the Flexible Conical Microstructured Film Inspired by the Streamlined Body Surface of the Pufferfish.

Given the challenges in accurately replicating the surface of the pufferfish, this study employed three-dimensional (3D) printing to create a model based on inverse modeling. The morphology of the pufferfish exhibits a streamlined configuration, characterized by a gradual widening from the anterior oral region to the central ocular area, followed by a progressive narrowing from the midabdominal region toward the caudal extremity. The RNG k-ε turbulence simulation results demonstrate that the streamlined body surface of the pufferfish diminishes differential pressure resistance. This enhancement promotes laminar flow formation, delays fluid separation, minimizes turbulence-induced vortices, and reduces frictional resistance. Moreover, the pufferfish's supple and uneven outer epidermis was simplified into a flexible, nonsmooth planar film to conduct fluid-solid coupling simulations. These revealed that the pufferfish's unique skin can absorb turbulent energy and minimize momentum transfer between the fluid and the solid film, lowering the fluid resistance during swimming. In summary, The high-efficiency swimming capacity of pufferfish stems not only from their streamlined body surface but also significantly from the unique structural characteristics and mechanical properties of their flexible skin. This research provides critical theoretical underpinnings for the design of functional bionic surfaces aimed at drag reduction.

Passage through a fishway entrance at different velocities—results from flume experiments with small non‐salmonids

AbstractA challenge in the design of fishways especially for large rivers is the trade‐off between attraction and passability of the entrance. High flow velocities in the entrance slot generate a strong attraction flow and tend to have better attraction efficiency for upstream migration. However, these velocities may also prevent small‐bodied species or juvenile fish from entering the fishway. With our experiment, we reproduced fish swimming behaviour and passage of a fishway entrance for small‐bodied fish under realistic hydraulic conditions. At an entrance slot with 0.4 m width four velocities 0.8, 1.2, 1.5 and 1.8 ms−1 were investigated. In total, 326 juvenile roach (Rutilus rutilus (Linnaeus, 1758)), gudgeons (Gobio gobio (Linnaeus, 1758)) and spirlins (Alburnoides bipunctatus (Bloch, 1782)) were tested. The passage rates of the three tested species were altogether higher than would have been predicted from reported swimming capacities. They increased from gudgeons to roach and (significantly) to spirlins and we could show how passage rates of the three species decreased with increasing flow velocities, suggesting species‐specific critical slot velocities. Still, these velocities are lower than those currently proposed to generate sufficient attraction flow, which may have implications for fishway design.

Effects of captive-breeding conditions on metabolic and performance traits in an endangered, endemic cyprinidontiform fish

Abstract Captive breeding and stocking are commonly employed strategies for enhancing fisheries and conserving endangered fish species. However, hatchery-raised fish often exhibit reduced performance in the wild, displaying alterations in physiological, morphological, and behavioral traits. We tested for differences in swimming capacity and metabolic traits between wild and hatchery-reared individuals of the Spanish toothcarp (Aphanius iberus) from 2 different populations. Furthermore, we experimentally tested if these changes translated into fitness differences after their stocking into the wild. There were significant differences in swimming capacity and metabolic traits between wild and hatchery-reared individuals and also between the 2 populations. Captive-bred individuals displayed consistently lower metabolic rates than wild individuals from the same population (30–76% lower). Critical swimming speed rather differed between the 2 populations. Sex-specific differences were observed in maximum and standard metabolic rates, with wild individuals and females generally exhibiting higher values but with some exceptions. During a 3-month experiment, survival rates did not significantly differ between wild and captive-bred fish. Captive-bred individuals started smaller but exhibited rapid growth during the experiment. Initially, larger captive-bred fish had lower body conditions than their wild counterparts, but these differences progressively diminished. In summary, captive-bred individuals of this fish species showed lower metabolic rates, although the differences with wild individuals slightly depended on sex and size.

Internal waves and fine-scale zooplankton sampling: Comment on Dewar-Fowler et al. (2023)

Dewar-Fowler et al. (2023; Mar Ecol Prog Ser 715:27-39) report on zooplankton carrying out short-term, unsynchronized migrations—or forays—into the surface layers. They used a motion-compensated zooplankton sampler consisting of an upward- and a downward-facing net. The sampler was deployed at a fixed depth, intending that individuals swimming into the nets are captured. We argue that it is warranted to account for internal waves when using this approach to address vertical swimming in zooplankton with weak swimming capacities.

Perceived and Real Water Competency and Drowning Risk Among Adults

Traditionally, public understanding of drowning survival has focused on swimming capacity, often measured by how far a person can swim. With respect to the high incidence of adult drowning in high-income countries, using the more inclusive concept of water competency may yield a more comprehensive explanatory framework for understanding the reasons behind these drownings. Any competency base required to survive a drowning situation is dynamic, complex, and multi-faceted. Furthermore, perceptions of risk and capacity to cope with that risk are likely to be pivotal to the avoidance of drowning. Adults’ perceived water competence was measured against their actual water competence in a pool environment. Adults were tested on their competence in entering and exiting water, floating, and swimming. Despite most adults (98%) being unable to swim more than 100 m in open water, more than half (59%) perceived themselves as good swimmers, and more than quarter (27%) thought they could swim more than 200 m at the post-survey. In conclusion, reality gaps were found in water competence among adult groups. Differences between perceived and actual competence suggest that many adults may underestimate the risk of drowning and overestimate their competency. This provides a plausible explanation as to why many adults drown.

Tolerance to fluctuating currents in farmed Atlantic salmon: a novel method to simulate offshore wave effects in the laboratory

To ensure acceptable animal welfare in emerging offshore Atlantic salmon aquaculture, it is crucial to understand the biological limits of the fish. Much work has been done on the effects of water currents, but few studies exist on waves, owing to logistical limitations. The purpose of this study was therefore to establish a method to replicate wave-like fluctuating water currents in the laboratory and quantify tolerance limits in salmon. To accomplish this, a swim tunnel system was modified so that current speeds could be programmed to automatically alternate between minimum and peak speeds of a desired magnitude and interval. The critical swimming speed (Ucrit) was subsequently measured in salmon of ~800 g as a baseline from which standardized tests of fluctuating currents could be prescribed. Fluctuating current trials were then performed using minimum speeds of 20% Ucrit and peak speeds of 80, 100, 120, and 140% Ucrit, and cycles of 0.5, 1, and 2 min. Fish were tested for 4 h or until they fatigued. All fish at 80 and 100% Ucrit endured 4 h of fluctuating currents. However, at 120% Ucrit, only 17% completed the test, and at 140% Ucrit, all fish became fatigued within 1.5 h, thus defining acute limits to fluctuating peak speeds. Wave interval did not affect fatigue times significantly. In conclusion, a novel method is introduced here to assess tolerances to various wave-like environments, showing that salmon can endure ~20% higher peak speeds in dynamic fluctuating currents when compared to known swimming capacities at constant speeds.

Spatiotemporal Variation Analyses of Water Quality, Fish Ecological Guilds, and the Longitudinal Connectivity in Musim Stream

The main objectives of this study were to analyze the spatiotemporal variations of water quality, fish ecological guilds and longitudinal connectivity in the weirs of Musim Stream. Fish were collected at 44 sites during spring and fall seasons for the analysis of trophic guilds, tolerance guilds, and the ecological stream health, based on FAI model. Total 33 species were observed in the stream and the dominant species were <i>Zacco platypus</i> and <i>Squalidus qracilis majimae</i>. Mean values of BOD and COD, as an indicator of organic matter pollution, showed high in the drought spring and low in fall (post-monsoon season). Also, TP showed same pattern with the BOD and COD pattern. The hierarchical clustering, based on the fish compositions, classified as three groups (up-stream, mid-stream and the down-stream). Based on the NMDS (Non-metric Multidimensional Scaling) analysis of fish abundance, high similarity was observed between S1-S5 sites, which were consistent with the results of hierarchical cluster analysis. Fish ecological guilds in the upper stream showed a high similarity between the insectivore and sensitive species, while in the middle and lower reaches, high similarity was found between the omnivore and intermediate species. Evaluations of longitudinal connectivity, based on the structural and hydrological characteristics of the weir, indicated all disconnections by the weirs, except for S15, in the spring. Stream ecological health, based on the FAI model, was significantly (<i>p</i> < 0.05, n=37) influenced by the height of overflow (<i>Hu1</i>). Overall, our study suggests that the longitudinal connectivity, based on the weir structures, flow regime and fish swimming capacity, were blocked off by the weirs in the stream. The results provide valuable insights for securing river management strategies and served as the fundamental data for stream restoration in the future.

A review of adult salmon maximum swim performance

Salmonids undertake long and strenuous migrations that require aerobic endurance and anaerobic burst swimming. Anthropogenic activity and natural disasters can make already challenging migrations even more difficult. This reinvigorates a central question: what is the maximum swimming capacity of adult salmon species across environmental conditions? We synthesized literature on adult salmon swim performance ( Oncorhynchus spp. and Salmo salar) to unfold what is known about how biological (sex and body size) and physical (temperature) factors affect swimming in adult salmon. Maximum anaerobic swimming—bursting, jumping, and leaping—are among the least studied swim performances in adult salmonids. Commonly, swim performance has been measured using swim flumes, but evidently adult salmon can swim faster in the wild than this set-up can capture. We show that larger fish can outswim smaller ones, and that thermal sensitivity of swimming differs inter-and -intra-specifically. Unresolved are how anaerobic swimming differs across temperatures, between males and females and across maturity states. This information can be used to inform exercise physiology research and future management and mitigation actions necessary to conserve these iconic, economically valuable species.

Gender Differences in Cognitive Functions of Youth Water Polo Players

Water polo (WP) as a highly demanding contact team sport, requires from players to have well developed cognitive functions, similar as in other team sports. Following same rules for females and males it is important to realize differences between them, which may contribute to their sports success and help coaches to develop adequate training models. Therefore, the aim of this study was to compare cognitive functions between female and male youth WP players. There were 36 female (25%) and 106 male (75%) youth WP players aged 12 to 14 years enrolled in this study. Variables measured included anthropometric indices, specific functional swimming capacities and cognitive functions testings using the Stroop test. Females showed better psychomotor speed (Stroop Off) (females 61.79±6.79 s vs. males 64.83±8.31 s, p=0.048) and response inhibition (Stroop On) (females 73.44±10.74 s vs. males 78.67±14.82 s, p=0.025) than males. Female youth WP players showed better results in psychomotor speed, inhibitory control and motor speed compared to males, taking both age and gender into account. Such differences might be of interest for coaches in WP, as well as in different sports to help them develop appropriate training strategies for each athlete.

High levels of metacercarial infestation (family: Diplostomidae) do not affect host energetics and swimming performance in the Epaulette goby (Coryogalops sordidus, Gobiidae).

Parasites have deleterious effects on their hosts, often resulting in altered host behavior or increased energy expenditure. When organisms are exposed to suboptimal environments, parasite loading may increase. Microbialite pools along the warm temperate South African coastline have been hypothesized as refugia for Epaulette gobies (Coryogalops sordidus, Gobiidae) when they are outside of their previously known subtropical distribution. The aim of this study was to determine if C. sordidus individuals infected with metacercarial cysts display higher metabolic rates or different swimming behavior compared to noninfected individuals. We measured each goby's swimming performance using a critical station-holding speed (Ucrit) test (n = 60) and visually scored their swimming behavior (n = 52) during these measurements. Also, we measured the metabolic rate of gobies using an intermittent flow respirometer system to determine standard metabolic rate (SMR) and maximum metabolic rate (MMR) from gobies at 21°C before and after swimming trials. Metacercarial load carried by infected gobies seemingly had no impact on the host's energetics (SMR or MMR), swimming ability (as repeated Ucrit tests), or swimming behavior compared to noninfected gobies. Thus, the metacercarial intensity observed in gobies in the current study appeared to have no impact on host swimming performance or behavior. Furthermore, the swimming capacity observed for C. sordidus, in general, suggests that this goby is a poor swimmer compared to other gobiid species.

Atlantic salmon in chronic turbulence: Effects on growth, behaviour, welfare, and stress

An increasing number of aquaculture companies aim to relocate Atlantic salmon (Salmo salar) farms to offshore locations exposed to large wave action. However, the impact that this environment will have on the fish reared within remains unknown, raising questions about their coping ability and welfare. The purpose of this study was to address this knowledge gap and understand how Atlantic salmon respond to wave-induced turbulence. For 8 weeks, post-smolts were exposed to chronic turbulence created using specially designed wave-generating equipment. During the trial, feed intake, growth, behavioural conditioning, plasma biochemistry and welfare were monitored. At study end, swimming capacities were determined, and vertebral deformities were assessed in a sub-group of fish. Despite both control and turbulence groups doubling their weights, the turbulence group exhibited a 5% reduction in size. A drop- in appetite, 17% across the first five days, and deviant behaviour was observed for the first 3 weeks in the turbulence group. These disparities disappeared in the latter half of the trial, suggesting the fish acclimatized. Welfare scores, haematological parameters, critical swimming speeds and deformity prevalence remained similar across both groups, suggesting the fish were not impacted by chronic turbulence on a physical or physiological level. In conclusion, these findings demonstrate that Atlantic salmon is a robust species with the capacity to adapt to novel more chaotic environments without suffering substantial reductions in welfare or long-term production performance. This suggests that Atlantic salmon will be able to adapt and thrive in wave-exposed turbulent offshore farm environments.

Inhibited swimming capacity of fish entrained in wake vortices behind a semi-cylinder

The loss of fish swimming capacity induced by those energetic vortical structures is detrimental, and limited research on the mechanism of fish entrainment in the vortex has been reported. The present study investigated the entrainment of a fish in vortices generated in wake of a semi-cylinder. The swimming fish was modeled using an undulatory NACA0012 airfoil behind semi-cylinders with a diameter D from 0.1 to 2.4 times the fish body length (L). It was found that the fish couldn't get higher propulsion from the low momentum flow for D > 0.8L. The wake of the fish was distorted by the shedding vortices and trailing-edge vortices (TEVs) arose at fishtail. The length- and time-scale of TEVs were positive correlated with these of oncoming co-directional vortices. The uncontrollable hydrodynamics induced by TEVs could elucidate the disruption of fish stability by surrounding vortices, manifested as a loss of propulsion, the generation of a unidirectional torque, and increased power consumption. The fish head gradually got energy from the turbulent flow of oncoming vortices, but fails to compensate adverse effects of the TEVs. The recovery of swimming capacity occurred only in the less disturbed flow between the wake vortices.

Spontaneous regeneration of cholecystokinergic reticulospinal axons after a complete spinal cord injury in sea lampreys

In contrast to humans, lampreys spontaneously recover their swimming capacity after a complete spinal cord injury (SCI). This recovery process involves the regeneration of descending axons. Spontaneous axon regeneration in lampreys has been mainly studied in giant descending neurons. However, the regeneration of neurochemically distinct descending neuronal populations with small-caliber axons, as those found in mammals, has been less studied. Cholecystokinin (CCK) is a regulatory neuropeptide found in the brain and spinal cord that modulates several processes such as satiety, or locomotion. CCK shows high evolutionary conservation and is present in all vertebrate species. Work in lampreys has shown that all CCKergic spinal cord axons originate in a single neuronal population located in the caudal rhombencephalon. Here, we investigate the spontaneous regeneration of CCKergic descending axons in larval lampreys following a complete SCI. Using anti-CCK-8 immunofluorescence, confocal microscopy and lightning adaptive deconvolution, we demonstrate the partial regeneration of CCKergic axons (81% of the number of axonal profiles seen in controls) 10 weeks after the injury. Our data also revealed a preference for regeneration of CCKergic axons in lateral spinal cord regions. Regenerated CCKergic axons exhibit colocalization with synaptic vesicle marker SV2, indicative of functional synaptic connections. We also extracted swimming dynamics in injured animals by using DeepLabCut. Interestingly, the degree of CCKergic reinnervation correlated with improved swimming performance in injured animals, suggesting a potential role in locomotor recovery. These findings open avenues for further exploration into the role of specific neuropeptidergic systems in post-SCI spinal locomotor networks.

Swimming Performance of Schizothorax sp. and Planning of Fish Passages in Nepal

Swimming performance of migrating fish is crucial for the planning and design of effective fish passages. Commonly the fish swimming speeds are classified into five categories: 1) Optimum Swimming Speed, 2) Maximum Sustained Swimming Speed, 3) Critical Swimming Speed, 4) Maximum Domed Swimming Speed, and 5) Burst Swimming Speed. Swimming characteristics are related to the different species' adaptions to the environmental conditions. A variety of different factors affect the swimming capacity of fish which is directly related to fish size and species-specific morphology. A crucial abiotic factor is water temperature, which affects the fish physiology and thereby the swimming speed of the fish. This means that the fish's swimming speed can increase with increased water temperature. The swimming speed also increases with fish size. Fish often use burst speeds when passing a step in a fish ladder or entering a culvert. Burst speed is the highest speed attainable for fish, and it is the maximum energy output to be gained by using the white muscle (red muscle in salmonids). The burst speed can only be maintained for short periods of time (seconds). In the Himalayas, the main objective of many fisheries development plans is to secure future habitats and ensure up- and downstream migration of fish. Schizothorax richardsonii is often a targeted species in the area due to its importance as a food resource. If a fish passage is prepared for the upstream migration of fish, it is also crucial for the fish population development to ensure a safe downstream passage. Data on swimming performance for many of the native fish species in Nepal are scarce which complicates the design of suitable ecologically adapted fishways. In general, nature-like fish passes allow most of the species to migrate, followed by vertical slot fishways, meander fishways, and thereafter fish ladders of the pool-and-weir type. The key factor is to construct fish-passes that under all naturally required environmental circumstances can allow appropriate passage conditions of the targeted fish species.

Assessing the disparity: comparative toxicity of Copper in zebrafish larvae exposes alarming consequences of permissible concentrations in Brazil

ABSTRACT Copper (Cu) is a naturally occurring metal with essential micronutrient properties. However, this metal might also pose increased adverse environmental and health risks due to industrial and agricultural activities. In Brazil, the maximum allowable concentration of Cu in drinking water is 2 mg/L. Despite this standard, the impact of such concentrations on aquatic organisms remains unexplored. This study aimed to evaluate the toxicity of CuSO4 using larval zebrafish at environmentally relevant concentrations. Zebrafish (Danio rerio) larvae at 72 hr post-fertilization (hpf) were exposed to nominal CuSO4 concentrations ranging from 0.16 to 48 mg/L to determine the median lethal concentration (LC50), established at 8.4 mg/L. Subsequently, non-lethal concentrations of 0.16, 0.32, or 1.6 mg/L were selected for assessing CuSO4 -induced toxicity. Morphological parameters, including body length, yolk sac area, and swim bladder area, were adversely affected by CuSO4 exposure, particularly at 1.6 mg/L (3.31 mm ±0.1, 0.192 mm2 ±0.01, and 0.01 mm2 ±0.05, respectively). In contrast, the control group exhibited values of 3.62 mm ±0.09, 0.136 mm2 ±0.013, and 0.3 mm2 ±0.06, respectively. Behavioral assays demonstrated impairments in escape response and swimming capacity, accompanied by increased levels of reactive oxygen species (ROS) and lipid peroxidation. In addition, decreased levels of non-protein thiols and reduced cellular viability were noted. Data demonstrated that exposure to CuSO4 at similar concentrations as those permitted in Brazil for Cu adversely altered morphological, biochemical, and behavioral endpoints in zebrafish larvae. This study suggests that the permissible Cu concentrations in Brazil need to be reevaluated, given the potential enhanced adverse health risks of exposure to environmental metal contamination.