Sweet chestnut forests in Europe are impacted by management abandonment, non-native species invasions and diseases, among other factors. Understanding the effects of these factors is crucial for forecasting future biodiversity changes, as well as proposing appropriate planning and management strategies. We studied sweet chestnut (Castanea sativa Mill.) and black locust (Robinia pseudoacacia L.) dominated forests within three hilly landscapes (Euganean hills, Montello, and Berici hills) of north-eastern Italy. We surveyed 25 paired sweet chestnut and black locust dominated plots of 100 m2 representing coppice or over-aged stands. We collected and analysed data related to vascular plant composition and richness, soil physical features (moisture content, shear strength and penetration resistance), ammonia-oxidising archaea in the soil, and stand structure features. Composition of vascular plants differed significantly between the two forest types, without the influence of management regime. Soil characteristics did not change comparing forest types, management regimes and their interaction, except for soil moisture that was higher in coppice forests. Ammonia-oxidising archaea abundance was lower in sweet chestnut stands. Sweet chestnut and black locust forests have a similar stand structure. The management regime played a role in determining mean diameter, number of stems and deadwood volume. We suggest adopting a heterogeneous array of silviculture practices to achieve the highest variety of forest structures and plant composition in Mediterranean hilly landscapes, coupled with management practices aimed at black locust control.