Swab Kit Research Articles

272 Relationships of salivary cortisol and automated brush system usage in freestall housed dairy cattle

Abstract The dairy industry has seen a significant shift in animal welfare practices, with growing emphasis on providing optimal living conditions for indoor-housed animals. Intensive management of dairy cows has become increasingly common, offering a controlled setting that ensures the well-being and productivity of the cow. Enrichment strategies such as automated brushes are proposed as a means for intensively managed dairy cows to improve their physical, mental, and emotional well-being. These behavioral benefits can contribute to an enriched living environment. Additionally, automated brushes have been shown to decrease cortisol concentrations which can lead to impacts such as heightened immune systems and increased productivity. With this known, the objective of this study was to evaluate the usage of an automated brush systems influence on cortisol concentrations of lactating dairy cows housed in a freestall setting. Cows (n = 29) over three trials (20 d each) were evaluated for duration of brush activity utilizing scan sampling of video recordings (0500 h-0900 h and 1700 h-2100 h; post milking) totaling 8 h/d throughout each trial. Animals were managed in accordance with standard operating procedures of the dairy, and all data was passively collected without influencing behavioral changes. Video capture was performed using a four camera closed-circuit media recording system. Cameras were placed in the corners of the pen, allowing full view of animals interacting with the brush system. Video images were scored using NOLDUS Observer XT. Saliva collection was performed during milking sessions using Salimetrics SalivaBio Children’s Swab kits. Salivary samples were analyzed using commercially available cortisol ELISA kits. Results indicate that cows exhibited greater cortisol concentrations in the mornings compared with the evenings (P = 0.018) and showed increased brush usage in the evenings compared with the mornings (P = 0.019). These findings suggest a pattern where cows tend to have increased cortisol concentrations in the mornings and engage in more brush usage behavior during the evenings. An analysis was conducted to examine relationships between brush usage and cortisol concentration, specifically focusing on the effects of automated brush usage on cortisol levels. Cortisol concentration was only slightly related to brush usage (R2 = 0.03; P ≤ 0.01). Partitioning the trial in halves provided insight of continuous brush usage and its relation to cortisol. Results illustrate (d 1 to 10), brush usage and cortisol were slightly correlated (R2 = 0.13; P ≤ 0.01) during the first half of the study, while there was no relationship noted during the second half (R2 = 0.002; P = 0.22). In conclusion, this study provides valuable insights into the relationship between automated brush systems and cortisol in lactating dairy cows housed in a freestall setting. Though these findings contrast those in literature, this details the need for additional research to elucidate brush usage impact on dairy cow hormonal status.

Pilot postal birth cohort HCV Screening in UK primary care.

Birth cohort screening has been implemented in some countries to identify the potentially 'missed population' of undiagnosed chronic Hepatitis C Virus (HCV) in people who may not be found through targeted approaches. To determine uptake of HCV antibody testing using an oral swab screening method, overall yield, whether those testing positive had risk markers in their primary care record, and cost per case detected. Pilot screening study set in general practices in the Southwest, South London and Yorkshire and Humber. Participants consenting were sent an oral swab kit in the post and saliva samples were tested for antibody to HCV. 16,436/98,396 (16.7%) patients consented and were sent an oral swab kit. 12,216 (12.4%) returned a kit, with 31 participants (yield 0.03%) testing positive for HCV antibody. 45% of those positive had a risk marker for HCV on their primary care record. Two (yield 0.002%) were confirmed RNA positive and referred for treatment, both had HCV risk markers. Cost per case detected was £16,000 per HCV antibody and £247,997 per chronic HCV. Wide-scale screening could be delivered and identified people infected with HCV, however most of these individuals could have been detected through lower-cost targeted screening. Yield and cost per case found were substantially worse than model estimates and targeted screening studies. Birth cohort screening should not be rolled out in primary care in England.

Evaluation of a novel university-based testing platform to increase access to SARS-CoV-2 testing during the COVID-19 pandemic in a cohort study

ObjectiveWe aimed to evaluate the feasibility and utility of an unsupervised testing mechanism, in which participants pick up a swab kit, self-test (unsupervised) and return the kit to an on-campus...

Exploitation of a 1D coordination polymer as a portable kit for an eye-catching fluorometric response towards sensing of trivalent cations.

The development and utilization of coordination polymers (CPs) have drawn interest for potential applications in different fields. Detection of metal ions in efficient and selective manners is an important field of research. It paves the way to protect human health by balancing toxic metal ions and biologically active metal ions in the atmosphere. In this regard, a new one-dimensional (1D) 4-(1-naphthylvinyl)pyridine (4-nvp) based CP [Cd(NCS)2(4-nvp)2]n (1) was synthesized and characterized structurally by single-crystal X-ray diffraction. Interestingly, this 1D CP underwent supramolecular aggregation via π⋯π stacking interactions, which specifically generated an environment for a potent "turn on" response in the presence of trivalent cations (Fe3+, Al3+, and Cr3+) in the nanomolar range but remained silent in the presence of other metal ions. Density functional theory (DFT) computations and X-ray photoelectron spectroscopy (XPS) were performed to establish the sensing phenomena. Fascinatingly, utilizing the sensitivity of 1 in an aqueous medium, a hands-on portable cotton swab kit was developed for instant identification of these three important trivalent metal cations.

2685. Impact of Education and Clinic Site Champion on Extragenital Testing for Sexually Transmitted Infection

Abstract Background Over the last several years sexually transmitted infections (STIs) have been on the rise in the United States. A potential area for improving the diagnosis of STIs is appropriate testing of extragenital (EG) sites (i.e., throat or rectum). These sites may serve as a reservoir for infection and lead to increased transmission if not identified and treated. Prior studies have shown that in men who have sex with men, genital testing alone misses >70% of STIs. In other patient groups, this may range from 10-40%. The aims of this project were twofold: to determine the proportion of patients receiving EG STI testing, and to observe the effect of a two-phase educational intervention on EG site testing. Methods Phase I (Jul-Dec 2021) included four urgent care clinics within a Midwest health system. Retrospective baseline data was ascertained for all adults receiving STI testing for Chlamydia trachomatis (CT) and Neisseria gonorrhea (GC) at these clinics. Next, all four clinics received access to an educational presentation regarding the importance of EG testing for STI detection. Two of these clinics served as pilot sites for the implementation of patient self-collection kits. Data was compiled for all adults undergoing STI testing at these clinics for a 6-month period. Phase II (Jul 2022-Mar 2023) involved further expansion and utilization of a site champion at several family medicine clinics including on-site promotion of multi-site testing and patient self-collection. Educational materials as in Phase I were provided to site champions Results Phase I baseline data indicated that less than 1% of samples from patients receiving STI testing were obtained from EG sites. There was a small increase in EG site testing after the educational intervention and implementation of self-collected swab kits (Table 1). Phase II data showed an increase in EG testing after the utilization of a clinic site champion and the sharing of testing data in family medicine clinics (Table 2). Conclusion Rates of STIs have reached an all-time high in the United States. EG infections serve as potential sources for STI transmission. Adequate multi-site testing is necessary. Implementation was most effective when a clinic site champion was identified, and clinic testing data was provided to them. Disclosures All Authors: No reported disclosures

Evaluation of urine drug screen falsification of results among patients with opioid use disorder receiving treatment in a telehealth model of care

IntroductionAs telehealth models for treatment of opioid use disorder (OUD) are expanding, the field does not know the reliability of urine drug screening (UDS) in this setting. The objective of this study is to determine the rate of falsification of UDS testing among patients with OUD in active treatment with buprenorphine via a telehealth provider. MethodsThis is a prospective cohort study of 899 randomly selected eligible patients, of which 392 participated in the final cohort that the study team used for analysis. The study mailed patients a UDS cup and asked them to return the sample by mail. After the UDS sample was received, a buccal swab was mailed, and the study asked patients to schedule a virtual meeting in which consent was sought and an observed buccal swab was obtained. We evaluated urine for evidence of falsification, and used buccal swabs to genetically match individuals to urine samples. ResultsAfter exclusion criteria, 395 (52.3 %) of 755 patients who received a UDS kit returned it for analysis prior to knowledge of the study. Of that, 392 samples had sufficient quantity for testing. We determined 383 (97.7 %) to be human urine containing buprenorphine without indication of exogenous buprenorphine addition and with evidence of compliance. A total of 374 patients received a buccal swab kit and 139 (37.2 %) attended the consent/observed buccal swab session. One hundred and thirty-two patients consented and completed the swab under video observation, and 120 successfully sent the swab back to the external laboratory. Of the 120 buccal swabs received, 109 (90.8 %) were a genetic match, 10 (8.3 %) were indeterminate, and 1 (0.8 %) was a genetic mismatch. ConclusionsThis study of patients treated by a telehealth OUD provider demonstrated a low rate of urine test falsification.

Diagnostic evaluation of nasopharyngeal swab and saliva kits against SARS-CoV-2: Adequate rapid screening is deemed necessary to overcome COVID-19 Pandemic

SARS-CoV-2 is the virus associated with the disease called COVID-19 and become a global pandemic. The only way to prevent its severe scenarios is through timely and rapid testing. In comparison to more time taking gold-standard RT-PCR testing, rapid diagnostic kits are used. For better prevention and diagnosis of SARS-CoV-2, the analysis of rapid diagnostic kits' accuracy and specificity is necessary. This study is meant to assess and examine the viability, responsiveness, and explicitness of quick antigen distinguishing nasopharyngeal swabs (NPS), and saliva-based units. The study was conducted on 200 suspected COVID-19 patients from Islamabad, 100 of which were RT-PCR positive while 100 were RT-PCR negative. For the analysis of Rapid diagnostic COVID-19 kits (RDT), nasopharyngeal swabs (NPS) and saliva samples were taken from the RT-PCR positive and negative patients. Among 100 RT-PCR positive patients, 62% were males (19 - 91 years), 34% were females (20 - 78 years) and 4% were children (6 - 17 years). False-negative results were significantly more observed in saliva-based RDTs of the sample (49%) as compared to nasopharyngeal swab RDT (38%). There were 2% invalid results in saliva-based RDT and 3% invalid results in Nasopharyngeal swab RDT. While among 100 RT-PCR negative patients 69% were males (19 - 80 yrs), 27% were females (18 – 77 yrs) and 4% were children (12 - 16 yrs.). False positive results were significantly more in saliva-based RDT (22%) as compared to Nasopharyngeal swab RDT (13%). The sensitivity and specificity of saliva-based RDT were 67% and 87% respectively while that of Nasopharyngeal swab (NPS) was 72% and 82% respectively, both of which were less than the gold standard RT-PCR sensitivity demanding the introduction of more sensitive RDT kits in Pakistan for accurate detection of COVID-19.

Monitoring SARS-CoV-2 in air and on surfaces and estimating infection risk in buildings and buses on a university campus.

BackgroundEvidence is needed on the presence of SARS-CoV-2 in various types of environmental samples and on the estimated transmission risks in non-healthcare settings on campus.ObjectivesThe objective of this research was to collect data on SARS-CoV-2 viral load and to examine potential infection risks of people exposed to the virus in publicly accessible non-healthcare environments on a university campus.MethodsAir and surface samples were collected using wetted wall cyclone bioaerosol samplers and swab kits, respectively, in a longitudinal environmental surveillance program from August 2020 until April 2021 on the University of Michigan Ann Arbor campus. Quantitative rRT-PCR with primers and probes targeting gene N1 were used for SARS-CoV-2 RNA quantification. The RNA concentrations were used to estimate the probability of infection by quantitative microbial risk assessment modeling and Monte-Carlo simulation.ResultsIn total, 256 air samples and 517 surface samples were collected during the study period, among which positive rates were 1.6% and 1.4%, respectively. Point-biserial correlation showed that the total case number on campus was significantly higher in weeks with positive environmental samples than in non-positive weeks (p = 0.001). The estimated probability of infection was about 1 per 100 exposures to SARS-CoV-2-laden aerosols through inhalation and as high as 1 per 100,000 exposures from contacting contaminated surfaces in simulated scenarios.SignificanceViral shedding was demonstrated by the detection of viral RNA in multiple air and surface samples on a university campus. The low overall positivity rate indicated that the risk of exposure to SARS-CoV-2 at monitored locations was low. Risk modeling results suggest that inhalation is the predominant route of exposure compared to surface contact, which emphasizes the importance of protecting individuals from airborne transmission of SARS-CoV-2 and potentially other respiratory infectious diseases.ImpactGiven the reoccurring epidemics caused by highly infectious respiratory viruses in recent years, our manuscript reinforces the importance of monitoring environmental transmission by the simultaneous sampling and integration of multiple environmental surveillance matrices for modeling and risk assessment.

Reagents and swab tests during the COVID-19 Pandemic: An optimized supply chain management with UAVs

In this paper, we develop a supply chain optimization model for the preparation, provision, transportation, and execution of swab tests during COVID-19 pandemic. The proposed approach is based on a multi-tiered network consisting of manufacturing companies of reagents, processing laboratories (where the swab kits are prepared and some swab tests are analyzed), landing stations for UAVs and test centers. As innovations in the supply chain, the sharing of reagents between processing laboratories and the use of UAVs, using 5G technology, are contemplated in the management of the COVID-19 Pandemic. To obtain the optimal solutions of the underlying optimization problem, we provide a variational formulation problem for which results of existence and uniqueness will be provided. Finally, some numerical simulations are examined to validate the effectiveness of our approach.

S568 Pre-Operative SARS-CoV2 Screening With TMA Nasopharyngeal Swab in Asymptomatic Patients Undergoing Non-Urgent Outpatient Procedures

Introduction: Pre-operative SARS-CoV2 nasopharyngeal swab screening is performed prior to non-urgent procedures in many institutions in the United States. It remains unknown whether a standardized screening algorithm is effective in capturing asymptomatic SARS-CoV2 carriers to limit exposure to other patients and staff. Our aim was to study the effectiveness of the screening approach used in our center to identify these patients. Methods: We performed a retrospective study at our center between 06/03/2020 and 11/18/2020. Firstly, patients were screened for typical symptoms of SARS-CoV2 by phone using a standard questionnaire. Those asymptomatic were then required to undergo transcription mediated amplification (TMA) testing within 72 hours of the procedure. Lastly, those with negative TMA were contacted again for another phone screening 24 hours prior to the procedure. All our patients were tested using the same SARS-CoV2 nasopharyngeal swab kit. Number of false negative tests were estimated by computing pre-test odds and negative likelihood ratio. The pre-test probability among asymptomatic patients was determined by dividing the number of positive TMA by all those tested in the same setting, assuming those who tested positive had the disease for 14 days. The negative likelihood ratio was calculated based on sensitivity (98.5%) and specificity (99%) of the TMA test that was used. Descriptive statistics were used to analyze the entire study period, including both the pre-surge (until 10/6/2020) and post-surge (after 10/6/2020) period. Results: A total of 8925 SARS-CoV2 TMA tests were performed as part of the pre-operative screening for non-urgent procedures during the study period. Of those, 67 patients tested positive. This represents a positivity rate of 0.75% and an estimated prevalence of 740 per 100,000. In the pre-surge period, 27 of 6355 patients tested positive (positivity rate 0.42%; prevalence 410 per 100,000), while in the post-surge period, 40 of 2570 patients tested positive (positivity rate 1.6%, prevalence 1540 per 100,000). Based on post-test odds, the number of false negatives during the entire study was estimated to be less than 1 (0.39 for pre-surge and 0.62 for post-surge period). The calculated number of false negative tests within the study period was 1.01. Conclusion: A three-tier pre-operative SARS-CoV2 screening approach including nasopharyngeal TMA testing among patients undergoing non-urgent procedures, effectively captures the overwhelming majority of asymptomatic cases.

The One Hour COVID Test: A Rapid Colorimetric Reverse-Transcription LAMP-Based COVID-19 Test Requiring Minimal Equipment.

At this writing, over 100 million people have tested positive for Corona Virus Disease-19 (COVID-19), and the global death toll from this disease has reached nearly 3 million. Despite the many tests currently available, we have not yet achieved the testing capacity needed to limit the spread of the virus and mitigate suffering worldwide. We have developed the One Hour COVID Test to address this challenge. Our test leverages an easy-to-use, commercially available oral swab kit for sample collection paired with a novel RNA processing protocol and a simple colorimetric assay that requires minimal equipment. The test can be easily scaled via automation and takes 1 h from sample collection to result.

Characterization of Spacesuit Associated Microbial Communities and Their Implications for NASA Missions.

BackgroundCrewed National Aeronautics and Space Administration (NASA) missions to other solar system bodies are currently being planned. One high-profile scientific focus during such expeditions would be life detection, specifically the discovery of past or present microbial life, if they exist. However, both humans and associated objects typically carry a high microbial burden. Thus, it is essential to distinguish between microbes brought with the expedition and those present on the exploring planets. Modern spacesuits are unique, customized spacecraft which provide protection, mobility and life support to crew during spacewalks, yet they vent, and the mobility of microbes through spacesuits has not been studied.ResultsTo evaluate the microbial colonization of spacesuits, NASA used an Extravehicular Activity swab kit to examine viable microbial populations of 48 samples from spacesuits using both traditional microbiological methods and molecular sequencing methods. The cultivable microbial population ranged from below the detection limit to 9 × 102 colony forming units per 25 cm2 of sample and also significantly varied by the location. The cultivable microbial diversity was dominated by members of Bacillus, Arthrobacter, and Ascomycota. However, 16S rRNA-based viable bacterial burden ranged from 105 to 106 copies per 25 cm2 of sample. Shotgun metagenome sequencing revealed the presence of a diverse microbial population on the spacesuit surfaces, including Curtobacterium and Methylobacterium from across all sets of spacesuits in high abundance. Among bacterial species identified, higher abundance of Cutibacterium acnes, Methylobacterium oryzae, and M. phyllosphaerae reads were documented.ConclusionThe results of this study provide evidence that identical microbial strains may live on the wrist joint, inner gauntlet, and outer gauntlet of spacesuits. This raises the possibility, but does not confirm that microbial contaminants on the outside of the suits could contaminate planetary science operations unless additional measures are taken. Overall, these data provide the first estimate of microbial distribution associated with spacesuit surfaces, which will help future mission planners develop effective planetary protection strategies.

Detection of bacterial pathogens using home oropharyngeal swab collection in children with cystic fibrosis.

Collection of respiratory cultures for airway microbiology surveillance is an essential component of routine clinical care in cystic fibrosis (CF). The COVID-19 global pandemic has necessitated increased use of telehealth, but one limitation of telehealth is the inability to collect respiratory specimens. We initiated a protocol for at-home collection of oropharyngeal (OP) swabs from children with CF. Home respiratory specimen collection was offered during telehealth encounters. Home OP swab kits were sent to participating families via mail with instructions for collection and return. Specimens were returned by overnight shipping or dropped off at a hospital lab for processing and culture. We evaluated demographic data and compared culture results from the home-collected specimen to the most recent specimen collected in clinic. We also tracked the frequency of newly identified Pseudomonas aeruginosa. Home OP swab kits were sent to families of 33 children with CF (range 1.5-19 years). OP swab kits were successfully returned from 19 children (range 1.5-19 years). One or more CF pathogens grew from 79% of the specimens. For four individuals, the home collected specimen demonstrated the new growth of P. aeruginosa. Home collection of OP swabs for bacterial culture is feasible in children with CF across a range of ages. Most home-collected specimens demonstrated growth of one or more CF pathogens and results were similar to recent in-clinic specimens, suggesting acceptable sample collection technique. Anti-pseudomonal therapy was initiated for four children based on the growth of P. aeruginosa from the home respiratory specimen.

Abstract P816: Nurse-Driven Rapid Covid-19 Testing in Emergency Department Stroke Patients

Introduction: The COVID-19 pandemic presents obstacles to time sensitive emergencies, such as stroke care. In acute strokes, knowing the COVID-19 status may help to preserve personal protective equipment (PPE) in patients in whom a thrombectomy may be indicated and helps to decrease unnecessary exposure. This study aims to demonstrate that rapid evaluation of a patient’s COVID-19 status is feasible without delaying treatment times. Methods: An intradisciplinary team was convened to create a workflow for rapid COVID-19 testing. The Abbott Rapid® COVID-19 swab kit and assay were stocked in the ED Pyxis, utilizing the narcotic count feature to ensure all swabs were accounted. Upon activation of Code Stroke, the ED RN donned PPE and swabbed the patient’s naso-oral pharynx. The collected swab was labeled, placed in a bio-hazard bag, sanitized and handed to a second RN outside of the room. The specimen was taken to a pre-alerted lab technician who prepped the assay after hearing the code stroke. After specimen collection, the patient followed the normal code stroke pathway and was taken to the CT scanner. Metrics were analyzed for the pre COVID-19 (January through April) and during active COVID-19 (May through July) periods. Results: There were 136 code strokes from January thru July 2020. 81 were during pre-COVID vs. 55 during active-COVID. 47 of 55 (96%) were swabbed, 2 (4%) of whom were positive. There was no difference between pre-COVID and active-COVID door to CT initiated time (16 mins [IQR 13-24] vs. 22 mins [IQR 13-25] p=0.75), door to CT resulted time (21 mins [IQR 15-26]) vs. 23 mins [IQR 16-29] p=0.63). 18 patients received tPA pre-COVID and 5 during active-COVID with no difference in DTN (pre: 37.5 mins [IQR 30-43] vs. active: 28 mins {IQR 26-41] p=0.37). Door to CT initiated was faster for those who had their COVID swab performed pre-CT (14 mins [IQR 11.5-16.5] p=0.034) vs. post-CT (20 mins [IQR 17-28]). Likewise, door to CT resulted was also faster pre-CT: 24 mins [IQR 19-32] vs. post-CT: 17 mins [IQR 15-23] (p=0.04). Conclusion: The COVID-19 rapid swab code stroke process was feasible and did not delay treatment times.

Evaluating Specimen Quality and Results from a Community-Wide, Home-Based Respiratory Surveillance Study.

While influenza and other respiratory pathogens cause significant morbidity and mortality, the community-based burden of these infections remains incompletely understood. The development of novel methods to detect respiratory infections is essential for mitigating epidemics and developing pandemic-preparedness infrastructure. From October 2019 to March 2020, we conducted a home-based cross-sectional study in the greater Seattle, WA, area, utilizing electronic consent and data collection instruments. Participants received nasal swab collection kits via rapid delivery within 24 hours of self-reporting respiratory symptoms. Samples were returned to the laboratory and were screened for 26 respiratory pathogens and a housekeeping gene. Participant data were recorded via online survey at the time of sample collection and 1 week later. Of the 4,572 consented participants, 4,359 (95.3%) received a home swab kit and 3,648 (83.7%) returned a nasal specimen for respiratory pathogen screening. The 3,638 testable samples had a mean RNase P relative cycle threshold (Crt ) value of 19.0 (SD, 3.4), and 1,232 (33.9%) samples had positive results for one or more pathogens, including 645 (17.7%) influenza-positive specimens. Among the testable samples, the median time between shipment of the home swab kit and completion of laboratory testing was 8.0 days (interquartile range [IQR], 7.0 to 14.0). A single adverse event occurred and did not cause long-term effects or require medical attention. Home-based surveillance using online participant enrollment and specimen self-collection is a safe and feasible method for community-level monitoring of influenza and other respiratory pathogens, which can readily be adapted for use during pandemics.

Use of swab for DNA sampling from confiscated raptors for molecular sexing

The objective of the study was to evaluate the feasibility and efficiency of using swab to collect tracheal and cloacal epithelial cells of confiscated raptor bird for genetic studies. Commercial swab kits were used to collect samples from 34 individuals of 10 raptor species, and as comparison blood samples from the same individual were also analyzed. FavorPrep™ Blood Genomic DNA Extraction Mini Kit dan FavorPrep™ Tissue Genomic DNA Extraction Mini Kit were used respectively to extract DNA from blood and epithelial cells. All DNA extracted from blood were successfully amplified for assignment of sex. On the other hand, the DNA extracted from buccal and cloacal swabs were only respectively 71% and 9% successfully amplified. This result suggests the potential used of buccal swabs for genetics studies of raptor, with further optimization for a better result.

Analysis of the Virus Contamination and Disinfection Effect in Isolation Ward of Patients With COVID-19.

The recent outbreak of COVID-19 has infected a large number of patients, increasing the importance of adequate disinfection of the hospital environment. We conducted this study to explore environmental virus contamination and the effect of terminal disinfection in the isolation ward of patients with COVID-19. A swab kit was used to sample various surfaces in the isolation and observation wards using the smear method. The samples were immediately sent to the PCR department of the laboratory for nucleic acid detection of COVID-19. We analyzed 31 high-frequency contact sites in three isolation wards of actively sick patients, of which seven were positive (22.58%, 7/31). Positive sites included the transfer window, bed rail, buffer room door handle, toilet door handle, and toilet faucet. All 55 samples taken from the wards of cured patients and the wards after terminal disinfection were negative. Virus contamination in areas frequently touched by patients in the isolation ward was high, so the awareness of correct disinfection must be increased. Use of 1,000–2,000 mg/L chlorine-containing disinfectant in the isolation ward was effective.

Microbiome and Metagenome Analyses of a Closed Habitat during Human Occupation.

Microbial contamination during long-term confinements of space exploration presents potential risks for both crew members and spacecraft life support systems. A novel swab kit was used to sample various surfaces from a submerged, closed, analog habitat to characterize the microbial populations. Samples were collected from various locations across the habitat which were constructed from various surface materials (linoleum, dry wall, particle board, glass, and metal), and microbial populations were examined by culture, quantitative PCR (qPCR), microbiome 16S rRNA gene sequencing, and shotgun metagenomics. Propidium monoazide (PMA)-treated samples identified the viable/intact microbial population of the habitat. The cultivable microbial population ranged from below the detection limit to 106 CFU/sample, and their identity was characterized using Sanger sequencing. Both 16S rRNA amplicon and shotgun sequencing were used to characterize the microbial dynamics, community profiles, and functional attributes (metabolism, virulence, and antimicrobial resistance). The 16S rRNA amplicon sequencing revealed abundance of viable (after PMA treatment) Actinobacteria (Brevibacterium, Nesternkonia, Mycobacterium, Pseudonocardia, and Corynebacterium), Firmicutes (Virgibacillus, Staphylococcus, and Oceanobacillus), and Proteobacteria (especially Acinetobacter) on linoleum, dry wall, and particle board (LDP) surfaces, while members of Firmicutes (Leuconostocaceae) and Proteobacteria (Enterobacteriaceae) were high on the glass/metal surfaces. Nonmetric multidimensional scaling determined from both 16S rRNA and metagenomic analyses revealed differential microbial species on LDP surfaces and glass/metal surfaces. The shotgun metagenomic sequencing of samples after PMA treatment showed bacterial predominance of viable Brevibacterium (53.6%), Brachybacterium (7.8%), Pseudonocardia (9.9%), Mycobacterium (3.7%), and Staphylococcus (2.1%), while fungal analyses revealed Aspergillus and Penicillium dominance.IMPORTANCE This study provides the first assessment of monitoring cultivable and viable microorganisms on surfaces within a submerged, closed, analog habitat. The results of the analyses presented herein suggest that the surface material plays a role in microbial community structure, as the microbial populations differed between LDP and metal/glass surfaces. The metal/glass surfaces had less-complex community, lower bioburden, and more closely resembled the controls. These results indicated that material choice is crucial when building closed habitats, even if they are simply analogs. Finally, while a few species were associated with previously cultivated isolates from the International Space Station and MIR spacecraft, the majority of the microbial ecology of the submerged analog habitat differs greatly from that of previously studied analog habitats.

Efficacy of a topical herbal and mineral formulation (Dynamiclear) for the treatment of herpes simplex labialis in the community setting: study protocol for a randomised, double-blind placebo-controlled trial

IntroductionHerpes simplex labialis (HSL) is a common infection that can cause painful lesions on the oral mucosa, commonly referred to as cold sores. Current biomedical treatments include topical aciclovir, which...

Estimating Diphenylamine in Gunshot Residues from a New Tool for Identifying both Inorganic and Organic Residues in the Same Sample

A method involving the collection and determination of organic and inorganic gunshot residues on hands using on-line in-tube solid-phase microextraction (IT-SPME) coupled to miniaturized capillary liquid chromatography with diode array detection (CapLC-DAD) and scanning electron microscopy coupled to energy dispersion X-ray (SEM-EDX), respectively, for quantifying both residues was developed. The best extraction efficiency for diphenylamine (DPA) as the main target among organic residues was achieved by using a dry cotton swab followed by vortex-assisted extraction with water, which permits preservation of inorganic residues. Factors such as the nature and length of the IT-SPME extractive phase and volume of the sample processed were investigated and optimized to achieve high sensitivity: 90 cm of TRB-35 (35% diphenyl, 65% polydimethylsiloxane) capillary column and 1.8 mL of the processed sample were selected for the IT-SPME. Satisfactory limit of detection of the method for analysis of DPA deposited on shooters’ hands (0.3 ng) and precision (intra-day relative standard deviation, 9%) were obtained. The utility of the described approach was tested by analyzing several samples of shooters’ hands. Diphenylamine was found in 81% of the samples analyzed. Inorganic gunshot residues analyzed by SEM-EDX were also studied in cotton swab and lift tape kit samplers. Optical microscopy was used to see the inorganic gunshot residues in the cotton swab samplers. The lift tape kits provided lesser sensitivity for DPA than dry cotton swabs—around fourteen times. The possibility of environmental and occupational sources could be eliminated when DPA was found together with inorganic residues. Then, the presence of inorganic and organic residues in a given sample could be used as evidence in judicial proceedings in the forensic field.