Sustaining Bloch Surface Waves Research Articles

Enhanced fluorescence detection of miRNA by means of Bloch surface wave-based biochips.

We report on the use of biochips based on one-dimensional photonic crystals sustaining Bloch surface waves to specifically detect target miRNA that is characteristic of hemorrhagic stroke (miR-16-5p) at low concentration in a buffer solution. The biochips were functionalized with streptavidin and ssDNA oligonucleotides to enable miRNA detection. To discriminate the target miRNA from a non-specific control (miR-101a-3p), we made use of an optical platform developed to work both in label-free and fluorescence detection modes. We demonstrate that the limit of detection provided when operating in the fluorescence mode allows us to specifically detect the target miRNA down to 1 ng mL-1 (140 pM), which matches the recommendations for diagnostic miRNA assays, 5 ng mL-1. The low costs open the way towards the application of these disposable optical biochips based on 1DPC sustaining Bloch surface waves as a promising tool for early disease detection in a liquid biopsy format.

Bioassay engineering: a combined label-free and fluorescence approach to optimize HER2 detection in complex biological media.

We report on the combined label-free/fluorescence use of one-dimensional photonic crystals to optimize cancer biomarker detection in complex biological media. The optimization of the assay working parameters permits us to maximize the final response of the biosensor. The detection approach utilizes a sandwich assay, in which one-dimensional photonic crystals sustaining Bloch surface waves are modified with monoclonal antibodies in order to guarantee high specificity during biological recognition. The multiple outcomes generated by such optimization experiments permitted us to determine the effective capture efficiency and the repeatability of the immobilization process, which was estimated to be close to 5%. By exploiting the resolution of the fluorescence operation mode, we studied non-specific interactions in different blocking agents, different analyte diluting buffers, and diverse concentrations of the detection antibody. As a clinically relevant biomarker, we selected the trans-membrane receptor tyrosine kinase HER2. HER2 regulates a variety of cell proliferation, growth, and differentiation pathways and its over-expression occurs in approximately 20-30% of breast cancer worldwide. As a final application, we transferred all the optimized working parameters to HER2 cancer biomarker assays in a complex biological environment. The label-free and fluorescence results obtained by analyzing MCF-7 (HER2 low positive) and 32D (HER2 negative) cell lysates demonstrate that we can successfully discriminate the two lysates.

Label-Free Monitoring of Human IgG/Anti-IgG Recognition Using Bloch Surface Waves on 1D Photonic Crystals.

Optical biosensors based on one-dimensional photonic crystals sustaining Bloch surface waves are proposed to study antibody interactions and perform affinity studies. The presented approach utilizes two types of different antibodies anchored at the sensitive area of a photonic crystal-based biosensor. Such a strategy allows for creating two or more on-chip regions with different biochemical features as well as studying the binding kinetics of biomolecules in real time. In particular, the proposed detection system shows an estimated limit of detection for the target antibody (anti-human IgG) smaller than 0.19 nM (28 ng/mL), corresponding to a minimum surface mass coverage of 10.3 ng/cm2. Moreover, from the binding curves we successfully derived the equilibrium association and dissociation constants (KA = 7.5 × 107 M−1; KD = 13.26 nM) of the human IgG–anti-human IgG interaction.

Hybrid inorganic/organic photonic crystal biochips for cancer biomarkers detection

We report on hybrid inorganic/organic one-dimensional photonic crystal biochips sustaining Bloch surface waves. The biochips were used, together with an optical platform operating in a label-free and fluorescence configuration simultaneously, to detect the cancer biomarker Angiopoietin 2 in a protein base buffer. The hybrid photonic crystals embed in their geometry a thin functionalization poly-acrylic acid layer deposited by plasma polymerization, which is used to immobilize a monoclonal antibody for highly specific biological recognition. The fluorescence operation mode is described in detail, putting into evidence the role of field enhancement and localization at the photonic crystal surface in the shaping and intensification of the angular fluorescence pattern. In the fluorescence operation mode, the hybrid biochips can attain the limit of detection 6 ng/ml.

Slot Waveguide Enhanced Bloch Surface Waves

The paper presents a novel concept for the on-chip integration of a multilayer platform sustaining Bloch surface waves further enhanced by the so-called slot waveguide effect. Through simulations, we demonstrate that a carefully designed polymer waveguide arrangement coated with a subwavelength dielectric multilayer can be efficiently used to first excite a Bloch surface wave at the surface of the multilayer and second to enhance this wave and allow longer propagation of the surface mode by constructive superimposition of two evanescent tails inside a narrow gap.

Bloch Surface Waves Biosensors for High Sensitivity Detection of Soluble ERBB2 in a Complex Biological Environment.

We report on the use of one-dimensional photonic crystals to detect clinically relevant concentrations of the cancer biomarker ERBB2 in cell lysates. Overexpression of the ERBB2 protein is associated with aggressive breast cancer subtypes. To detect soluble ERBB2, we developed an optical set-up which operates in both label-free and fluorescence modes. The detection approach makes use of a sandwich assay, in which the one-dimensional photonic crystals sustaining Bloch surface waves are modified with monoclonal antibodies, in order to guarantee high specificity during the biological recognition. We present the results of exemplary protein G based label-free assays in complex biological matrices, reaching an estimated limit of detection of 0.5 ng/mL. On-chip and chip-to-chip variability of the results is addressed too, providing repeatability rates. Moreover, results on fluorescence operation demonstrate the capability to perform high sensitive cancer biomarker assays reaching a resolution of 0.6 ng/mL, without protein G assistance. The resolution obtained in both modes meets international guidelines and recommendations (15 ng/mL) for ERBB2 quantification assays, providing an alternative tool to phenotype and diagnose molecular cancer subtypes.

Detection of soluble ERBB2 in breast cancer cell lysates using a combined label-free/fluorescence platform based on Bloch surface waves

We report on the use of one-dimensional photonic crystals to detect clinically relevant concentrations of ERBB2/neu/Her2 in cell lysates. ERBB2 is a pivotal breast cancer biomarker and targetable oncogenic driver associated with aggressive breast cancer subtypes. To quantitate soluble ERBB2, we developed an optical platform that combines label-free and fluorescence detection modes. Such platform makes use of a sandwich assay in which the one-dimensional photonic crystals sustaining Bloch surface waves are tailored with a monoclonal antibody for highly specific biological recognition (BSW biochip). In a second step, a second antibody to ERBB2 quantitatively detects the bound analyte. The strategy of the present approach takes advantage of the combination of label-free and fluorescence techniques, making bio-recognition more robust and sensitive. In the fluorescence operation mode, the platform can attain the limit of detection 0.3ng/mL (1.5pM) for ERBB2 in cell lysates. Such resolution meets the international guidelines and recommendations (15ng/mL) for diagnostic ERBB2 assays that in the future may help to more precisely assign therapies counteracting cancer cell proliferation and metastatic spread.

Experimental investigation of the propagation properties of bloch surface waves on dielectric multilayer platform

BackgroundThe periodic dielectric multilayers sustaining Bloch surface waves have been proposed as a platform for the sensing applications and the two dimensional integrated optics. In this paper, we present the experimental and theoretical investigation of propagation properties of Bloch surface waves, for example propagation length and refractive index of the surface mode, at the interface of a dielectric multilayer platform. We use thin layers (~λ/25) of titanium dioxide as an additional layer of high index material.MethodsWe exploit multi-heterodyne scanning near-field optical microscopy and total internal reflection configuration as a near-field and far-field characterization tools.ResultsThe longest propagation length is achieved when the multilayer is designed to have the dispersion curve positioned close to the middle of the photonics band gap. We measure a Bloch surface wave mode of propagation length 3.24 mm and of an effective refractive index contrast 0.15.ConclusionsThe experimental results are in conformity with theoretical results. This study paves a way to realize efficient and compact two dimensional components and systems.

Near-field characterization of a Bloch-surface-wave-based 2D disk resonator.

We present, to the best of our knowledge, the first experimental investigation of a two-dimensional disk resonator on a dielectric multilayer platform sustaining Bloch surface waves. The disk resonator has been patterned into a few tens of nanometer thin (∼λ/25) titanium dioxide layer deposited on the top of the platform. We characterize the disk resonator by multi-heterodyne scanning near-field optical microscopy. The low loss characteristics of Bloch surface waves allowed us to reach a measured quality factor of 2×103 for a disk radius of 100 μm.

Two-dimensional polymer grating and prism on Bloch surface waves platform.

A one-dimensional photonic crystal sustaining Bloch Surface Waves (BSWs) is used as a platform for two-dimensional integrated optics. The dielectric platform shows low loss, long propagation distance and high surface field enhancement. In order to study the potential of the platform for future photonic chips, polymer ultra-thin prisms and gratings (~100 nm) are engineered on the top of the platform. This polymer layer modifies the BSWs effective index enabling a direct manipulation of light. The BSW deflection effects caused by surface prisms are observed in the near-field and Snells law is verified; then the BSW diffractions through surface gratings are experimentally and theoretically characterized. The results show a robust platform that can be used for integrated optics that includes different optical components. One of the main advantages is that these 2D photonic devices can have arbitrary shapes, which is difficult to obtain in 3D.

Real time secondary antibody detection by means of silicon-based multilayers sustaining Bloch surface waves

We report on time resolved sensing experiments demonstrating the direct, label-free and specific binding of an Antibody/Anti-antibody system probed by means of Bloch surface waves on silicon-based one-dimensional photonic crystals. The selectivity of the device is verified by monitoring the adsorption of Anti Mouse IgG (non-specific target) and Anti Rabbit IgG (specific target) on rabbit IgG receptors previously transferred by micro contact printing on the surface of 1DPC devices. The limits of the sensitivity are explored by varying the concentration of the tested solutions. Based on a modified ELISA assay, we performed a quantitative estimation of the number of detected antibodies adsorbed at the probed surface in both the non-specific and specific case (∼10 4 and ∼10 8, respectively), as associated to measured spectral shifts in the sensorgram.