Accurate evaluation of grapevine water use is essential for optimizing water management and maximizing grapevine yield and berry quality in Mediterranean climates. Understanding the water and heat flux dynamics in a vineyard during grapevine berry maturation is of utmost importance. This study focuses on evaluating sensible and latent energy fluxes at the canopy, the soil beneath the canopy, and the interrow areas. The primary objective is to develop a model framework for accurately estimating these energy fluxes, contributing to a better understanding of their behavior during berry ripening. The model’s accuracy was assessed by comparing the estimated fluxes with those measured by an eddy-covariance system installed at a reference height of three meters in the experimental vineyard. This validation step was essential to confirm the model’s ability to capture the intricate energy flux dynamics of the vineyard, especially during grape maturation. The results revealed a high level of agreement between the observed and estimated fluxes, confirming the model’s reliability. This comprehensive evaluation of energy fluxes provides valuable insights for optimizing irrigation strategies. By doing so, this study contributes to improving grape quality, ensuring sustainable water resource use, and ultimately enhancing vineyard productivity in arid and water-scarce regions.