Abstract This study examines 50 years of land use planning practices in Florianópolis, Brazil, to identify the attributes that resulted in the city’s notable and long-standing commitment to pro-conservation programmes and policies. By analysing key policies, planning strategies, and partnerships that underpin the preservation of forests and green spaces, this research contributes valuable insights to the ongoing discourse on the combined role of conservation governance and collective action in building sustainable and resilient cities. The social-ecological systems framework is applied to evaluate the interactions between multi-level laws, collective action groups, and mid-level government officials to determine the drivers that promoted incremental and accumulative change, ultimately leading to sustainable land conservation practices in Florianópolis. The findings highlight the importance of stakeholder goal alignment, managerial flexibility, and collaboration with diverse actors in achieving long-term conservation goals, particularly in cities adopting an amenity-led growth model. Growing challenges to the continued protection of conservation units in the city resulting from population growth are also raised to advance approaches to evaluating the durability of sustainability initiatives.