The aim of the present study was to determine the prevalence of Toxoplasma gondii IgG and IgM antibodies in patients who were admitted in Abant İzzet Baysal University Education and Research Hospital between January 2010 and December 2016 with a suspicion of toxoplasmosis. Anti-T. gondii IgM and IgG antibodies and IgG avidity test determined by ELISA method in 14,262 serum samples belonging to the Abant İzzet Baysal University Education and Research Hospital were retrospectively investigated. IgG was detected in 4079 serum samples with 78% negative, 21% positive, and 0.8% gray zone. IgM was detected in 13,671 cases with 98% negative, 1.2% positive, and 0.5% gray zone. (3.8%, n=540) and neurology (3.4%, n=478) patients who were referred to the Obstetrics and Gynecology Clinic (88.3%, n=12,588) for the majority of the cases requested for the detection of T. gondii antibodies. It has been found that a request has been made. The seropositivity of T. gondii has been found to be so large that it should not be ignored. It has come to the conclusion that the events in the risk group should be taken into consideration for this parasite, and awareness should be established. In recent years, this awareness has been observed, especially in neurology clinics.