Susceptibility Testing Of Dermatophytes Research Articles

Isolation, Identification and Antifungal Susceptibility Testing Of Dermatophytes in Kimshrc, Bangalore

Isolation, Identification and Antifungal Susceptibility Testing Of Dermatophytes in Kimshrc, Bangalore

Identification and antifungal susceptibility testing of dermatophytes

Identification and antifungal susceptibility testing of dermatophytes

Comparison of Disk Diffusion and Broth Microdilution Methods for in Vitro Antifungal Susceptibility Testing of Dermatophytes

Comparison of Disk Diffusion and Broth Microdilution Methods for in Vitro Antifungal Susceptibility Testing of Dermatophytes

Evaluation of a modified EUCAST fragmented-mycelium inoculum method for in vitro susceptibility testing of dermatophytes and the activity of novel antifungal agents.

For antifungal susceptibility testing of nonsporulating or poorly sporulating dermatophytes, a fragmented-mycelium inoculum preparation method was established and compared to broth microdilution testing according to CLSI and EUCAST guidelines. Moreover, the in vitro activity of new antifungal agents against dermatophytes was evaluated. Agreement between the mycelial inoculum method and the CLSI broth microdilution method was high (93% to 100%). Echinocandins (minimal effective concentration [MEC], ≤0.5 mg/liter) and posaconazole (MIC, ≤3.00 mg/liter) showed good activity against all tested dermatophytes.

In vitro susceptibility testing of dermatophytes with their fragmented mycelia as inoculum

377 clinical isolates and seven dermatophyte culture collection strains were tested in vitro by microdilution against a panel of 18 antifungal agents. A newly introduced method for rapid and reliable inoculum preparation for moulds using the fragmented mycelia of freshly grown isolates as inoculum was firstly applied to dermatophytes. The performance of the method was tested with two different culture media which are recommended by different standardized testing methods for moulds. The standardized fragmented mycelia provided both, countable single colonies (viable units) on solid culture media and distinct readable endpoints (minimum inhibitory concentrations) in the microdilution wells for all tested topical and systemic drugs, including the echinocandins. As with moulds, and as shown by the culture collection strains, reproducible minimum inhibitory concentrations were obtained with an essential agreement (± 1 log2-dilution) of 97% to 100%, by a significant reduction of the overall testing time. As exemplarily shown for the microdilution technique, this inoculum method should also provide a solid basis for improved agar-based susceptibility testing methods such as disc-, tablet-, or strip-tests.

Susceptibility testing of dermatophytes

Susceptibility testing of dermatophytes

Interlaboratory evaluation of the Etest® for antifungal susceptibility testing of dermatophytes

Interlaboratory evaluation of the Etest® for antifungal susceptibility testing of dermatophytes

Antifungal Susceptibility Testing of Dermatophytes

Antifungal Susceptibility Testing of Dermatophytes

An ATP bioluminescence assay applicable to rapid fluconazole susceptibility testing of dermatophytes.

An ATP bioluminescence assay as a rapid reference method for fluconazole (FLCZ) susceptibility testing of dermatophytes, as well as yeasts, was developed and evaluated by comparing it with viability, turbidity and fungal protein content-based conventional methods. FLCZ susceptibility results obtained with strains of Candida albicans and dermatophytes by the bioluminescence method in high-resolution medium were well correlated with those obtained by conventional methods currently used in clinical microbiology laboratories or reported previously, including a broth dilution method by the National Committee for Clinical Laboratory Standards (NCCLS). Thus, ATP bioluminescence assay can be used to monitor fungal growth in liquid culture media. The procedure has considerable potential for the rapid testing of FLCZ susceptibility of dermatophytes and other fungi.