Survival Rate Of Piglets Research Articles

RpIFN-λ1 alleviates the clinical symptoms of porcine epidemic diarrhea

Porcine epidemic diarrhea (PED), caused by the porcine epidemic diarrhea virus (PEDV), primarily affects the jejunum and ileum of pigs. Interferons, glycoproteins with high species specificity and potent antiviral activity, are crucial in defending against viral infections. Unlike other interferons, interferon-lambda (IFN-λ) mainly acts on mucosal epithelial cells and exhibits robust antiviral activity at mucosal surfaces. However, the high cost limits the use of naturally extracted interferons in farming. In this study, we expressed recombinant porcine interferon-lambda 1 (rpIFN-λ1) in eukaryotic cells, demonstrating effective antiviral activity against PEDV in Vero E6 and IPI-FX cells. In vivo, rpIFN-λ1 alleviated clinical symptoms and intestinal damage, enhanced antioxidant capacity, reduced inflammation, and significantly improved the survival rate of piglets following PEDV infection. Both in vitro and in vivo studies confirmed that rpIFN-λ1 upregulated interferon-stimulated genes (ISGs) via the JAK-STAT pathway, thereby exerting antiviral effects. In conclusion, rpIFN-λ1 significantly inhibited PEDV replication and alleviated clinical symptoms. The selectivity of rpIFN-λ1 for intestinal cells and its ability to reduce viral shedding suggest that this agent is a promising antiviral for enteric viruses such as PEDV. Our findings highlight rpIFN-λ1 as a cost-effective, efficient, and novel strategy for antiviral treatment of PEDV.

Andrographolide inhibits porcine epidemic diarrhea virus by inhibiting the JAK2-STAT3 pathway and promoting apoptosis

Porcine epidemic diarrhea (PED) is an acute, virulent, and highly contagious disease caused by the porcine epidemic diarrhea virus (PEDV). The high mutation rate of PEDV makes it difficult to effectively control using traditional vaccines, emphasizing the need for novel anti-PEDV-specific drugs. Therefore, this study aimed to investigate the activity and mechanism of action of andrographolide (AND) against PEDV in vitro and in vivo. In vitro experiments showed that AND treatment significantly inhibited PEDV replication in a cell model. The mechanism is that AND treatment significantly suppressed PEDV-induced activation of the JAK2-STAT3 pathway, which promoted apoptosis and inhibited the proliferation of the virus. Moreover, PEDV-infected 3-day-old piglets were treated with AND, and clinical symptoms, intestinal morphology, and viral load were examined. In vivo experiments showed that AND treatment reduced clinical symptoms, ameliorated intestinal damage, and increased the survival rate of infected piglets by 16.7 %. Conclusively, this study contributes to the field of PEDV antiviral drug development and provides new directions for PED prevention and treatment.

Genetic Links between Reproductive Traits and Amino Acid Pairwise Distances of Swine Leukocyte Antigen Alleles among Mating Partners in Microminipigs.

Previously, we found that a greater dissimilarity in swine leukocyte antigen (SLA) class I and class II alleles between mating partners resulted in increased farrowing rates in a highly inbred population of Microminipigs (MMPs). In this follow-up study, we have analyzed the effects of dissimilarity in SLA alleles between mating partners for seven different reproductive traits, including litter size and the number of stillborn and live or dead weaned piglets. We determined the relationships among reproductive traits within each mating event and the amino acid distances of SLA alleles as markers of diversity between mating partners. Our results indicate that mating partners with greater amino acid pairwise genetic distances in the SLA-1 class I gene or DQB1 class II gene alleles were associated with significantly larger litter sizes and higher numbers of live piglets at birth and weaning. Also, partners with greater pairwise distances in the SLA-2 class I gene alleles exhibited fewer pre-weaning deaths. These findings suggest that the dissimilarity in SLA class I and class II alleles between mating partners may affect not only farrowing rates but also other key reproductive traits such as litter size and improved piglet survival rates. Consequently, SLA alleles could serve as valuable genetic markers for selecting mating partners in breeding programs and for conducting epistatic studies on various reproductive traits in MMPs.

Lizhong decoction inhibits porcine epidemic diarrhea virus in vitro and in vivo

Ethnopharmacological relevanceLizhong decoction (LZD) is a frequently utilized traditional Chinese remedy for diarrhea. It is unknown how effective it is as an antiviral against PEDV infection. Aim of the studyIn vitro and in vivo PEDV infection models were used to evaluate the anti-PEDV potential of LZD extract. Materials and methodsLC-MS was used for qualitative analysis of LZD. The antiviral effect of LZD against PEDV using flow cytometry (FC), Quantitative real-time polymerase chain reaction (QPCR), immunofluorescence assay (IFA) analysis in Vero and IPEC-J2 cells. Additionally, we measured the survival rate, clinical symptoms, body weights, fecal scores, temperature, histological analysis, and viral load in a model of newborn piglets infected with PEDV in order to assess the antiviral impact of LZD in vivo. ResultsIn total, 648 compounds were identified, including 144 Alkaloids, 128 Terpenoids, etc. LZD effectively suppressed PEDV replication in vitro. According to time of addition experiments, LZD mostly inhibited PEDV during the viral life cycle's replication stages. During PEDV infection, LZD can Significantly decrease the apoptotic rate of IPEC-J2 cells and Vero cells. In comparison to the model group, LZD was able to decrease the viral titers in the infected piglets' intestinal and visceral tissues, ameliorate their intestinal pathology, cause a significant increase in body weight growth and increase the piglet survival rate. ConclusionOur findings indicate that the aqueous solution derived from LZD suppressed PEDV replication both in vitro and in vivo, indicating its potential as a candidate for pharmaceutical development.

Effectiveness of the liquid method of feeding suckling piglets

The article examined the dependence of sow productivity, the growth rate of suckling piglets, their weight at transfer to rearing, the consumption of precursor feeds, and their costs on dry and liquid feeding systems for piglets in the suckling period. The dependence on feed costs for piglet feeding, veterinary drugs for prevention, and the costs and profitability of piglet rearing in these feeding systems were analyzed. It was found that the use of the liquid milk replacer Opticare Milk helped to improve the survival rate of piglets up to weaning by 7.5 %, increase their number in the sow's nest at this time by 7.0 %, increase the average daily growth of suckling piglets by 20.4 %, increase the absolute weight gain by 19.8 %, increase the weight of the piglet litter at weaning by 22.0 % and increase the individual weight of the piglets at weaning by 14.7 % compared to piglet litters fed dry granulated starter provide Superior Neonatal. Multiple fertility, high fertility, and litter weight of piglets at birth did not depend on how piglets were fed during the suckling period. With liquid feeding of suckling piglets, the proportion of piglets with diarrhea decreased by 12.20 % and the costs for the prevention of gastrointestinal diseases increased by 58.5 %, the costs for the treatment of gastrointestinal diseases by 55.4 %, and the veterinary costs for rearing a piglet during the suckling period by 53.8 % compared to analogs with dry feeding. To 1 kg of gain, these costs increased by 33.1 % due to prevention and 28.4 % due to treatment, resulting in a 28.5 % overrun of the total costs for treatment and prevention. In general, the costs for feeding and medical and preventive measures increased by 157.8 % per animal and 122.2 % per 1 kg of gain compared to dry feeding. At the same time, under the liquid feeding system for suckling piglets, the cost of keeping a sow per weaned piglet fell by 6.00 %. The proportion of these costs per piglet decreased by 21.6 %, and the cost of keeping a sow per 1 kg increased. Still, the cost of a piglet at weaning increases by 13.2 %, the cost of 1 kg live weight of piglets at weaning increases by 5.6 %, the share of feed and veterinary components in the cost of rearing a piglet increases by 9.1 %, and by 3.4 %. At the same time, the market value increases by 14.7 %, the income from rearing a piglet rises by 17.9 %, and the profitability of rearing a piglet increases by 1.95 %.

Genome-wide association analysis revealed new QTL and candidate genes affecting the teat number in Dutch Large White pigs.

Teat number (TNUM) is an important reproductive trait of sows, which affects the weaning survival rate of piglets. In this study, 1166 Dutch Large White pigs with TNUM phenotype were used as the research object. These pigs were genotyped by 50K SNP chip and the chip data were further imputed to the resequencing level. The estimated heritabilities of left teat number (LTN), right teat number (RTN) and total teat number (TTN) were 0.21, 0.19 and 0.3, respectively. Based on chip data, significant SNPs for RTN on SSC2, SSC5, SSC9 and SSC13 were identified using genome-wide association analysis (GWAS). Significant SNPs for TTN were identified on SSC2, SSC5 and SSC7. Based on imputed data, the GWAS identified a significant SNP (rs329158522) for LTN on SSC17, two significant SNPs (rs342855242 and rs80813115) for RTN on SSC2 and SSC9, and two significant SNPs (rs327003548 and rs326943811) for TTN on SSC5 and SSC6. Among them, four novel QTL were discovered. The Bayesian fine-mapping method was used to fine map the QTL identified in the GWAS of the imputed data, and the confidence intervals of QTL affecting LTN (SSC17: 45.22-46.20 Mb), RTN (SSC9: 122.18-122.80 Mb) and TTN (SSC5: 14.01-15.91 Mb, SSC6: 120.06-121.25 Mb) were detected. A total of 52 candidate genes were obtained. Furthermore, we identified five candidate genes, WNT10B, AQP5, FMNL3, NUAK1 and CKAP4, for the first time, which involved in breast development and other related functions by gene annotation. Overall, this study provides new molecular markers for the breeding of teat number in pigs.

Effects of dietary iron supplementation on reproductive performance of sows and growth performance of piglets.

This experiment aimed to investigate the effects of dietary iron supplementation from different sources on the reproductive performance of sows and the growth performance of piglets. A total of 87 sows with similar farrowing time were blocked by body weight at day 85 of gestation, and assigned to one of three dietary treatments (n = 29 per treatment): basal diet, basal diet supplemented with 0.2% ferrous sulfate (FeSO4), and basal diet supplemented with 0.2% iron sucrose, respectively, with 30% iron in both FeSO4 and iron sucrose. Compared with the control (CON) group, iron sucrose supplementation reduced the rate of stillbirth and invalid of neonatal piglets (P < 0.05), and the number of mummified fetuses was 0. Moreover, it also improved the coat color of newborn piglets (P < 0.05). At the same time, the iron sucrose could also achieve 100% estrus rate of sows. Compared with the CON group, FeSO4 and iron sucrose supplementation increased the serum iron content of weaned piglets (P < 0.05). In addition, iron sucrose increased serum transferrin level of weaned piglets (P < 0.05) and the survival rate of piglets (P < 0.05). In general, both iron sucrose and FeSO4 could affect the blood iron status of weaned piglets, while iron sucrose also had a positive effect on the healthy development of newborn and weaned piglets, and was more effective than FeSO4 in improving the performance of sows and piglets.

Genome-Wide Association Study Meta-Analysis Elucidates Genetic Structure and Identifies Candidate Genes of Teat Number Traits in Pigs.

The teat number is a pivotal reproductive trait that significantly influences the survival rate of piglets. A meta-analysis is a robust instrument, enhancing the universality of research findings and improving statistical power by increasing the sample size. This study aimed to identify universal candidate genes associated with teat number traits using a genome-wide association study (GWAS) meta-analysis with three breeds. We identified 21 chromosome threshold significant single-nucleotide polymorphisms (SNPs) associated with five teat number traits in single-breed and cross-breed meta-GWAS analyses. Using a co-localization analysis of expression quantitative trait loci and GWAS loci, we detected four unique genes that were co-localized with cross-breed GWAS loci associated with teat number traits. Through a meta-analysis and integrative analysis, we identified more reliable candidate genes associated with multiple-breed teat number traits. Our research provides new information for exploring the genetic mechanism affecting pig teat number for breeding selection and improvement.

Intranasal administration with recombinant vaccine PRVXJ-delgE/gI/TK-S induces strong intestinal mucosal immune responses against PDCoV

Porcine deltacoronavirus (PDCoV) is a novel coronavirus that causes enteric diseases in pigs leading to substantial financial losses within the industry. The absence of commercial vaccines and limited research on PDCoV vaccines presents significant challenges. Therefore, we evaluated the safety and immunogenicity of recombinant pseudorabies virus (PRV) rPRVXJ-delgE/gI/TK-S through intranasal mucosal immunization in weaned piglets and SPF mice. Results indicated that rPRVXJ-delgE/gI/TK-S safely induced PDCoV S-specific and PRV gB-specific antibodies in piglets, with levels increasing 7 days after immunization. Virus challenge tests demonstrated that rPRVXJ-delgE/gI/TK-S effectively improved piglet survival rates, reduced virus shedding, and alleviated clinical symptoms and pathological damage. Notably, the recombinant virus reduced anti-inflammatory and pro-inflammatory responses by regulating IFN-γ, TNF-α, and IL-1β secretion after infection. Additionally, rPRVXJ-delgE/gI/TK-S colonized target intestinal segments infected with PDCoV, stimulated the secretion of cytokines by MLVS in mice, stimulated sIgA secretion in different intestinal segments of mice, and improved mucosal immune function. HE and AB/PAS staining confirmed a more complete intestinal mucosal barrier and a significant increase in goblet cell numbers after immunization. In conclusion, rPRVXJ-delgE/gI/TK-S exhibits good immunogenicity and safety in mice and piglets, making it a promising candidate vaccine for PDCoV.

Genetic and phenotypic time trends of litter size, piglet mortality, and birth weight in pigs

IntroductionLitter size in pigs has increased steadily since 1990. Because of unfavorable genetic correlations with piglet mortality, breeding goals should include survival traits next to litter size. Unbalanced breeding programs that neglect this requirement have produced increased mortality levels, attracting negative public attention. Balanced breeding does not have this disadvantage, but the general public is largely unaware of this.MethodsWe present long-term time trends as realized in commercial breeding. The data includes (i) phenotypes of litter size, piglet birth weight, and piglet mortality, as used in routine breeding value estimation; and (ii) the genomic Best Linear Unbiased Prediction (gBLUP) estimated breeding values thus obtained. Piglet mortality (2001–2022) and birth weight (2009–2022) phenotypes were related to litter size by recording year. Estimated breeding values (EBVs) for the mortality traits were regressed on those for litter size by birth year (2012–2022).ResultsAverage litter size is very weakly correlated to the mortality (R2 ≤ 0.06) and birth weight (0.07 ≤ R2 ≤ 0.26) traits, and those correlations are unfavorable (antagonistic) within each year. However, all traits analyzed here show favorable simultaneous phenotypic and genetic trends over time: the antagonisms are neutralized by balanced breeding. Above the annual mean litter size level, farrowing and lactation mortality rates increased with increasing litter size in every year (unfavorable), but the annual intercepts and the slopes decreased from 2001 to 2022 (favorable). Average litter birth weight decreased with litter size in every year (unfavorable), but the annual intercepts increased and the slopes decreased from 2009 to 2022 (favorable). The within-litter birth weight variation coefficient increased with litter size in every year (unfavorable), but the annual intercepts decreased from 2009 to 2022 (favorable). The proportion of low birth weights (i.e.,&amp;lt; 0.9 kg) for a given litter size is decreasing over time, and the critical birth weight level (below which lactation mortality increases strongly) is clearly population dependent and changes over time too.DiscussionThe increases in litter size and piglet survival rates due to balanced breeding policies lead to reduced total numbers of dead piglets (i.e., per country, per year) coinciding with a certain pig production volume (i.e., with a certain total number of weaned piglets).

Genetic parameter estimation for pork production and litter performance traits of Landrace, Large White, and Duroc pigs in Japan.

We estimated genetic parameters for two pork production and six litter performance traits of Landrace, Large White, and Duroc pigs reared in Japan. Pork production traits were average daily gain from birth to end of performance testing and backfat thickness at end of testing (46,042 records for Landrace, 40,467 records for Large White, and 42,920 records for Duroc). Litter performance traits were number born alive, litter size at weaning (LSW), number of piglets dead during suckling (ND), survival rate of piglets during suckling (SV), total piglet weight at weaning (TWW), and average piglet weight at weaning (AWW) (27,410, 26,716, and 12,430 records for Landrace, Large White, and Duroc, respectively). ND was calculated as the difference between LSW and litter size at start of suckling (LSS). SV was calculated as LSW/LSS. AWW was calculated as TWW/LSW. Pedigree data for Landrace, Large White, and Duroc breeds contained 50,193, 44,077, and 45,336 pigs, respectively. Trait heritability was estimated via single-trait analysis and genetic correlation between two traits was estimated via two-trait analysis. When considering the linear covariate of LSS in the statistical model for LSW and TWW, for all breeds, the heritability was estimated to be 0.4-0.5 for pork production traits and below 0.2 for litter performance traits. Estimated genetic correlation between average daily gain and backfat thickness was small, ranging from 0.057 to 0.112, and those between pork production traits and litter performance traits were negligible to moderate, ranging from -0.493 to 0.487. A wide range of genetic correlation values among the litter performance traits was estimated, while that between LSW and ND could not be obtained. The results of genetic parameter estimation were affected by whether the linear covariate of LSS was included in the statistical model for LSW and TWW or not. This finding implies the necessity of carefully interpreting the results according to the choice of statistical model. Our results could give fundamental information on simultaneously improving productivity and female reproductivity for pigs.

Administration of ketoprofen in postpartum sows to control the incidence of post-parturient disorders and improve piglet survival rate.

Inflammation and pain management in postpartum hyperprolific sows is currently an important animal welfare issue in the swine industry. The present study investigates effects of ketoprofen treatment on the incidence of post-parturient disorders, feed intake, colostrum yield, piglet colostrum intake, colostrum immunoglobulin G (IgG) and piglet mortality rate during the first 3 days of postnatal life. In total, 61 Danish Landrace×Yorkshire crossbred sows and their offspring (n = 833) were included in the experiment. The sows were randomly distributed into two groups: i) control (n = 31), sows were treated with tolfenamic acid 2 mg per kg for 2 days postpartum; ii) ketoprofen (n = 30), sows were treated with ketoprofen 3 mg per kg for 2 days postpartum. The farrowing process of the sows was monitored for 24 h daily, and data associated with farrowing were collected. Piglet colostrum intake, sow colostrum yield and colostrum IgG were determined. During the first 3 days postpartum, the incidence of sows that had fever did not differ between control and ketoprofen groups (51.6% and 56.7%, respectively, p = 0.692). Piglet colostrum intake did not differ between control and ketoprofen groups (p = 0.736). However, the proportions of piglets that had inadequate colostrum intake were 71.3%, 22.6%, and 5.4% in those with birth weights of <1.0 kg, 1.0 to 1.29 kg, and ≥1.30 kg, respectively (p<0.001). The piglet mortality rate did not differ between control and ketoprofen groups (p = 0.808). Administration of ketoprofen in postpartum sows for 2 days can control the evidence of post-parturient disorders in sows as effectively as the use of tolfenamic acid. No deleterious effect of ketoprofen was detected on sow colostrum yield, piglet colostrum intake and piglet mortality. Therefore, ketoprofen can be recommended as an alternative anti-inflammatory drug used in postpartum sows.

Melatonin Supplementation during the Late Gestational Stage Enhances Reproductive Performance of Sows by Regulating Fluid Shear Stress and Improving Placental Antioxidant Capacity

In this study, the effects of daily melatonin supplementation (2 mg/kg) at the late gestational stage on the reproductive performance of the sows have been investigated. This treatment potentially increased the litter size and birth survival rate and significantly increased the birth weight as well as the weaning weight and survival rate of piglets compared to the controls. The mechanistic studies have found that these beneficial effects of melatonin are not mediated by the alterations of reproductive hormones of estrogen and progesterone, nor did the glucose and lipid metabolisms, but they were the results of the reduced oxidative stress in placenta associated with melatonin supplementation. Indeed, the melatonergic system, including mRNAs and proteins of AANAT, MTNR1A and MTNR1B, has been identified in the placenta of the sows. The RNA sequencing of placental tissue and KEGG analysis showed that melatonin activated the placental tissue fluid shear stress pathway to stimulate the Nrf2 signaling pathway, which upregulated its several downstream antioxidant genes, including MGST1, GSTM3 and GSTA4, therefore, suppressing the placental oxidative stress. All these actions may be mediated by the melatonin receptor of MTNR1B.

Sow Farrowing Early Warning and Supervision for Embedded Board Implementations.

Sow farrowing is an important part of pig breeding. The accurate and effective early warning of sow behaviors in farrowing helps breeders determine whether it is necessary to intervene with the farrowing process in a timely manner and is thus essential for increasing the survival rate of piglets and the profits of pig farms. For large pig farms, human resources and costs are important considerations in farrowing supervision. The existing method, which uses cloud computing-based deep learning to supervise sow farrowing, has a high equipment cost and requires uploading all data to a cloud data center, requiring a large network bandwidth. Thus, this paper proposes an approach for the early warning and supervision of farrowing behaviors based on the embedded artificial-intelligence computing platform (NVIDIA Jetson Nano). This lightweight deep learning method allows the rapid processing of sow farrowing video data at edge nodes, reducing the bandwidth requirement and ensuring data security in the network transmission. Experiments indicated that after the model was migrated to the Jetson Nano, its precision of sow postures and newborn piglets detection was 93.5%, with a recall rate of 92.2%, and the detection speed was increased by a factor larger than 8. The early warning of 18 approaching farrowing (5 h) sows were tested. The mean error of warning was 1.02 h.

Precision technologies in pig farming

The transition to digital, intelligent production technologies and robot-based systems is one of the priorities of the scientific and technological development of Russia. The extensive use of information technology and electronic automated systems is the basis of an intelligent manufacturing execution system that replaces a person, especially for objective and precise control over the quantity and quality of feed given to animals. Modern genetics has achieved a substantial increase in the number of piglets per litter, which does not allow a sow to fully meet their biological needs with her own milk. To preserve the health and high performance of all piglets born, effective and economically feasible methods and technologies are needed. The objective of the research was to analyze the efficiency of applying the components of precision pig farming in piglet feeding when using the feeding systems for suckling piglets CulinaCup and CulinaFlexpro. Their application has a positive effect on the growth and survival rate of suckling piglets due to proper and timely feeding. The systems meet all sanitary and hygienic standards and ensure efficient raising of piglets in large litters.

Relationship between dexamethasone treatment around parturition of primiparous sows and farrowing performance and newborn piglet traits

This study evaluated the relationship between the steroidal anti-inflammatory action of dexamethasone treatment in primiparous sows and farrowing and piglet performance in the first 5 d of life. For this purpose, 234 gilts were selected on the day of farrowing and distributed among three treatments: CON - control, without dexamethasone treatment; DexaPF - treatment with dexamethasone (20 mg im) per female at the moment of copious colostrum secretion (pre-farrowing); and DexaFO - treatment with dexamethasone (20 mg im), per female when the first piglet was born (farrowing onset). All females and their litters were evaluated regarding farrowing duration, obstetric interventions, colostrum yield and intake, newborn piglet traits, and piglet performance until 5 d of age. A subsample of 79 females (∼26 per treatment) had their blood glucose concentration evaluated hourly shortly after the first piglet was born until the end of farrowing. Additionally, blood samples from 11 litters per treatment were collected for immunocrit evaluation. As a result, faster farrowing was observed in the DexaPF treatment (188.14 min; P = 0.002) compared with CON (229.99 min) and DexaFO (221.79 min). Additionally, lower obstetric intervention rates were observed in sows treated with dexamethasone (DexaPF = 7.69%; DexaFO = 5.13%) compared with CON (19.23%; P = 0.02). The sow's blood glucose concentration during farrowing was higher in DexaPF (90.55 mg/dL) than in CON (73.15 mg/dL) and DexaFO (80.06 mg/dL) treatments (P < 0.01). Besides the effect on farrowing duration, no differences among treatments were observed regarding piglets born alive and stillbirths, newborn piglet vitality, colostrum consumption, immunocrit, and colostrum yield (P ≥ 0.17). Regarding piglet traits, higher percentages of piglets born without meconium staining and lower percentages of piglets with meconium scores 2 and 3 were observed in the groups treated with dexamethasone (DexaPF and DexaFO; P < 0.01) compared with CON. However, piglet weight gain and survival rate until 5 d of age were not affected by the treatment (P ≥ 0.61). In summary, dexamethasone treatment before farrowing onset, in primiparous sows, had the potential to reduce the farrowing duration and the necessity of obstetric intervention, but it did not affect the main productive parameters such as the occurrence of stillbirths, piglet weight gain, and survival rates until 5 d of age.

Effects of freezing storage on the stability of maternal cellular and humoral immune components in porcine colostrum

The placental structure of sows is epitheliochorial and prevents maternal serum immunoglobulin transfer to the fetus; therefore, the piglet relies on the ingestion of colostrum to acquire passive immunity. Colostral antibody-mediated and cell-mediated immunity contribute to immunity in piglets. However, little is known about the effects of freezing at −20 °C on colostral immune components during short-term storage, whether this will somehow compromise the acquisition of passive immunity of newborn piglets fed with this colostrum and the humoral immunity in porcine colostrum, and to possible shifts in immunological levels in colostrum collections during the colostral period. Based on the average concentration of immunoglobulin, frozen and fresh colostrum did not differ significantly. Overall, there were no storage differences in total macrophages, granulocytes, and NK cells. However, the frozen colostrum presented T lymphocyte subsets and B lymphocytes significantly lower than the fresh colostrum (p ≤ 0.05). Therefore, to sustain higher piglet survival rates, B cells may be a selective strategy to ensure immune defense to neonatal piglets. According to our findings, colostrum can be stored by freezing at −20 °C for up to 30 days and surplus porcine colostrum can be collected from the sow up to eight hours after the start of farrowing.

Effect of a combination of altrenogest and double PGF2α administrations on farrowing variation, piglet performance and colostrum IgG

The variation of gestation length in sows leads to difficulties performing farrowing supervision. The present study was performed to investigate whether oral administration of altrenogest until 112 days of gestation and double administration of PGF2α at 113 days of gestation can synchronise the onset of parturition in sows and minimise deleterious effects on the incidence of stillbirths and colostrum quality. Additionally, the effects of synchronised farrowing on colostrum yield and piglet birth weight, colostrum intake and survival rate of piglets until seven days of postnatal life were also investigated. In total, 193 Landrace x Yorkshire crossbred sows were randomly allocated according to parity number into two groups, i.e. control (n = 95) and treatment (n = 98). The control sows were allowed to farrow naturally. The treatment sows were orally administered 20 mg per day of altrenogest for four days from 109 to 112 days of gestation and were administered PGF2α twice on day 113 of gestation. Individual body weight at birth and 24 h after birth of piglets in all litters were determined in both control (n = 1609) and treatment (n = 1707) groups. Colostrum consumption of all piglets, colostrum yield, colostrum IgG and serum progesterone of sows were determined. On average, the total number of piglets born per litter were 17.0 ± 3.1. The proportion of sows farrowed before 114 days of gestation was higher in the control than the treatment group (8.4% and 2.0%, respectively, P = 0.05) and 92.8% of sows in the treatment group farrowed on day 114 of gestation. The percentage of stillborn piglets per litter did not differ significantly between control and treatment groups (4.5% and 4.6%, respectively). Colostrum yield of sows did not differ between control and treatment groups (5.52 ± 0.13 and 5.28 ± 0.12 kg, respectively, P = 0.174). However, colostrum intake of piglets was lower in the treatment than the control group (354.7 ± 6.6 and 381.2 ± 7.0 g, respectively, P = 0.012). Colostrum IgG was higher in the control than the treatment group (41.2 ± 1.1 and 37.3 mg per ml, P = 0.013). In conclusion, altrenogest treatment from 109 to 112 days and double administrations of PGF2α on day 113 of gestation can control gestation length in sows. No deleterious effects of this protocol on the incidence of stillbirths and sow colostrum yield were detected. However, piglet colostrum intake and colostrum IgG were compromised. Thus, care of newborn piglets in the treatment group should be considered.

The Effect of Lift Crates on Piglet Survival Rate and Sow Stress Level during Farrowing.

Simple SummaryIn intensive farms, the mortality of newborn piglets during farrowing is a critical aspect that represents a significant cause of economic loss in pig production, and the deaths, representing 5–25% of newborn piglets, depend mainly on starvation and crushing. In the present study, we aimed to determine the effect of lift farrowing crates on piglet mortality by crushing and on sow welfare. Eighty-four sows of the same genetics were observed over three sessions for one year. Twenty-eight sows per session were randomly assigned to a room equipped with conventional crates or lift crates. No primiparous sows were considered in the study to avoid sows unexperienced with the dynamics of lift crates. The farm’s veterinarian assessed the number of crushed piglets within 48 h, 72 h, and at weaning. Hair cortisol concentration (HCC) was measured at the entry and the exit from farrowing to evaluate stress level variation. Feet diseases and backfat thickness were evaluated to assess sows’ potential diseases induced by lift crates and metabolic problems. The results show that the number of crushed piglets per sow was higher in the conventional crate rooms than the lift crate ones. Mean values of HCC variation in sows during farrowing were significantly different in the two housing systems and higher for the lift crate-housed sows. No significant differences were detected for backfat variation and feet disease scoring. In conclusion, sows housed in the lift crates evidenced an increase in hair cortisol values during farrowing, probably reflecting a higher stress status induced by the lift crate.In the present study, we aimed to determine the effect of lift farrowing crates on piglet mortality by crushing and on sow welfare. Eighty-four sows were considered in the one-year experiment in three monitoring sessions. In each session, 14 sows were housed in a room with conventional crates (CC), and 14 sows were lodged in a room equipped with lift crates (LC). The sows, of the same genetics, with parity ranging from 2 to 9, were randomly distributed in CC and LC rooms. No primiparous sows were considered in the study to avoid sows unexperienced with the dynamics of lift crates. The numbers of crushed piglets, assessed by the farm’s veterinarian, within 48 h, 72 h, and at weaning (28th day), were recorded. Hair cortisol concentration (HCC) was measured upon entry and exit from farrowing to evaluate stress level variation. Feet diseases and backfat thickness were evaluated to assess sows’ potential diseases induced by lift crates and metabolic problems. The results show that the number of crushed piglets per sow was higher in the CC rooms than in the LC rooms in the first two days after delivery (0.39 vs. 0.15, p < 0.05) and up to weaning (0.50 vs. 0.37; p < 0.05). Mean values of HCC variation in sows during farrowing were significantly different in the two housing systems and higher for the LC sows (0.53 pg/mg vs. 0.22 pg/mg; p < 0.05). No significant differences were detected for backfat variation and feet disease scoring between LC and CC sows. In conclusion, LC sows evidenced an increase in hair cortisol values during farrowing, probably caused by a higher stress status induced by the lift crate, along with the benefit of the higher survival rate of piglets before weaning.

Heritability and genetic correlation estimates of semen production traits with litter traits and pork production traits in purebred Duroc pigs.

We estimated heritabilities of semen production traits and their genetic correlations with litter traits and pork production traits in purebred Duroc pigs. Semen production traits were semen volume, sperm concentration, proportion of morphologically normal sperms, total number of sperm, and total number of morphologically normal sperm. Litter traits at farrowing were total number born, number born alive, number stillborn, total litter weight at birth, mean litter weight at birth, and piglet survival rate at birth. Litter traits at weaning were litter size at weaning, total litter weight at weaning, mean litter weight at weaning, and piglet survival rate from birth to weaning. Pork production traits were average daily gain, backfat thickness, and loin muscle area. We analyzed 45,913 semen collection records of 896 boars, 6,950 farrowing performance records of 1,400 sows, 2,237 weaning performance records of 586 sows, and individual growth performance records of 9,550 animals measured at approximately 5 mo of age. Heritabilities were estimated using a single-trait animal model. Genetic correlations were estimated using a 2-trait animal model. Estimated heritabilities of semen production traits ranged from 0.20 for sperm concentration to 0.29 for semen volume and were equal to or higher than those of litter traits, ranging from 0.06 for number stillborn and piglet survival rate at birth to 0.25 for mean litter weight at birth, but lower than those of pork production traits, ranging from 0.50 for average daily gain to 0.63 for backfat thickness. In many cases, the absolute values of estimated genetic correlations between semen production traits and other traits were smaller than 0.3. These estimated genetic parameters provide useful information for establishing a comprehensive pig breeding scheme.