The effect of sharp forward-facing steps on boundary-layer transition is systematically investigated in this work in combination with the influence of variations in Mach number, Reynolds number and streamwise pressure gradient. Experiments have been conducted in a quasi-two-dimensional flow at Mach numbers up to 0.77 and chord Reynolds numbers up to 13 million in the Cryogenic Ludwieg-Tube Göttingen. The adopted experimental set-up allows an independent variation of the aforementioned parameters and enables a decoupling of their respective effects on the boundary-layer transition, which has been measured accurately and non-intrusively by means of a temperature-sensitive paint. The functional relations determined between a non-dimensional transition parameter and the non-dimensional step parameters allow the step effect on transition to be isolated from the influence of variations in Mach number, Reynolds number and pressure gradient. Criteria for acceptable heights of forward-facing steps on natural laminar flow surfaces for the examined test conditions are derived from the present functional relations. The measured transition locations are also correlated with the results of linear, local stability analysis for the smooth configuration, enabling the estimation of the step-induced increment of the amplification factor ΔN of Tollmien–Schlichting waves, which can be incorporated in the eN transition prediction method.