Surface Scanning Research Articles

Effect of magnetic resonance imaging protocol on decision-making for positioning of dental implants in edentulous cases.

This study aimed to assess the influence of magnetic resonance imaging (MRI) protocol on the decision-making for the positioning of dental implants in edentulous arches in comparison to planning based on cone beam computed tomography (CBCT). One phantom was scanned with CBCT and two MRI protocols (T1- and T2-weighted). Two calibrated examiners performed digital implant planning (coDiagnostiX, Dental Wings), considering a digital prosthetic planning and alveolar ridge surface scan. Four implants were planned for each patient dataset, and the angular deviation between the long axis of the implants and the prosthetic planning occlusal plane was measured. Each examiner planned 40 implants, 12 implants with CBCT and 28 implants with MRI (16 with T1 and 12 with T2 images). Significant differences in angle deviation were observed between CBCT, T1, and T2 in the anterior region for both examiners (p=0.009 for examiner 1 and p=0.042 for examiner 2). Implants planned with CBCT showed significantly lower angular deviation than those based on the T1 and T2 scans for both examiners (p=0.028 and p=0.046 for examiner 1 and p=0.028 and p=0.027 for examiner 2). No significant difference in angular deviation was found between T1 and T2 for both examiners (examiner 1: p=0.600, examiner 2: p=0.916). Compared to CBCT planning, the MRI protocol influenced decision-making for anterior dental implants, with angular deviations within acceptable clinical thresholds, but further studies are needed to validate these findings.

Asphalt pavement raveling recognition based on deep learning algorithms

Abstract Pavement raveling can lead to the development of issues such as potholes. Monitoring and providing real-time early warnings for road surface developments is crucial for timely maintenance planning and effectively controlling the occurrence and progression of surface issues. To achieve intelligent identification of raveling distress on asphalt pavement surfaces, we utilized a portable laser-based pavement surface scanning device to collect raveling images, followed by histogram equalization to eliminate noise caused by environmental factors. Additionally, data enhancement strategies such as rotation and mirroring were used to enrich training data and expand the sample size. A Convolutional Neural Network (CNN) was employed to perform multi-layer convolution and pooling operations on the images to quickly extract features of distress. Finally, five pre-trained transfer learning models—ResNet-18, DenseNet, VGG16, Inception, and EfficientNet—were introduced to identify the asphalt surface disease data and the normal surface data. The results showed that the accuracy and loss of the training sets of several models exhibited a positive trend, with the EfficientNet model demonstrating stable and superior performance under the same training conditions. The precision matrix, confusion matrix, precision, recall, and F1 score were used as evaluation indexes to assess the effectiveness of the models for identifying pavement raveling. The EfficientNet and Inception models had the best accuracy and comprehensive performance, with accuracy rates of 0.96 and 0.95, respectively. The precision, recall, and F1 score are 0.95, 0.96, and 0.96 for the EfficientNet model respectively. A total of 146 images correctly predicted raveling disease and only 4 normal images were incorrectly predicted as diseased. For example, the EfficientNet model’s identification accuracy for pockmark disease on the PC platform was 0.963, verifying the model’s feasibility in practical applications. The principal contribution of this paper lies in the efficient and precise identification of asphalt pavement raveling, which adversely affects vehicular traveling comfortability. This provides theoretical and technical support for the intelligent identification of asphalt pavement defects and enhancing road asset maintenance management in the pavement engineering industry.

On the Versatility of the sp-, sp 2-, and sp 3-Hybridized Chalcogen-Bearing Molecules To Engage in Type I Chalcogen···Chalcogen Interactions: A Quantum Mechanical Investigation of Like···Like and Unlike Complexes.

The predilection of sp-, sp 2-, and sp 3-hybridized chalcogen-bearing molecules to engage in type I chalcogen···chalcogen interactions was comparatively unveiled in like···like/unlike CY···YC, YCY···YCY, and F2Y···YF2 (where Y = O, S, and Se) complexes, respectively. Upon the optimized monomers, a potential energy surface (PES) scan was conducted to pinpoint the most favorable complexes. The energetic findings unveiled the ability of the investigated systems to engage in the interactions under study with binding energy values ranging from -0.36 to -2.33 kcal/mol. Notably, binding energies were disclosed to align in the posterior sequence; sp 2- (i.e., YCY···YCY) > sp- (i.e., CY···YC) > sp 3- (i.e., F2Y···YF2) hybridized complexes, except the like···like oxygen-bearing complexes. Instead, the highest negative binding energy values were detected for the OCO···OCO followed by those of the F2O···OF2 and CO···OC complexes. Furthermore, the like···like selenium-bearing complexes demonstrated the most considerable binding energies compared to the other investigated complexes. Remarkably, the quantum theory of atoms in molecules and noncovalent interaction index analyses revealed the highly directional and closed-shell nature of the investigated chalcogen···chalcogen interactions. Symmetry adapted-perturbation theory findings outlined the dispersion forces as the commanding forces for all the studied complexes. These observations will provide convincing justifications for the nature of chalcogens within type I chalcogen···chalcogen interactions, leading to increased progress in various domains regarding drug design and materials science.

Aerodynamic and acoustic evaluation of a cylinder covered by stretched grooved fabric near critical Reynolds numbers

This study investigates aerodynamic drag and acoustic noise generated from a circular cylinder by wrapping longitudinally grooved fabrics. The fabrics are stretched to different levels to explore their potential for flow and acoustic control near critical Reynolds numbers. To analyze the fabrics' surface characteristics, 3D scanning and several microscopic techniques were employed. The parallel-mounted load cells and an array of microphones were used to measure the drag and noise at Reynolds numbers, based on cylinder diameter, ranging from \num{3.3e4} to \num{1.1e5} in an anechoic wind tunnel. Additionally, hotwire anemometry was used to examine the wake structures in the vicinity of the cylinder. The surface scanning results revealed that stretching the fabric transversely to twice its original size reduced the groove depth by approximately $54\%$ and increased the width by around $25\%$, resulting in an overall reduction of the arithmetic surface roughness $S_a$ by $18.6\%$. Based on the wind tunnel data, it was observed that a grooved fabric with higher stretch, and thus lower surface roughness exhibited a minimum drag coefficient at a higher Reynolds number, along with reduced acoustic tonal noise levels.

Temporal lobe evolution in Hominidae and the origin of human lobe proportions.

Objectives Evolutionary changes in hominin social complexity have been associated with increases in absolute brain size. The temporal lobes are nestled in the middle cranial fossae (MCF) of the skull, the dimensions of which allow estimation of temporal lobe volume (TLV) in extant and fossil taxa. Materials and Methods The main aim of this study is to determine where along the hominid phylogeny, major temporal lobe size transitions occurred. We used computed tomography (CT) scans of crania, 3D photogrammetry data, and laser surface scans of endocranial casts to measure seven MCF metrics in 11 extant anthropoid taxa using multiple regressions to estimate TLV in 5 extant hominids and 10 fossil hominins. Phylogenetic comparative methods mapped temporal lobe size, brain size, and temporal lobe proportions onto phylogenetic trees broadly for Hominidae and specifically for Hominini. Results Extant Homo sapiens were not an outlier in relative brain size, temporal lobe size, or proportions of the temporal lobes, but some proportions within the lobe were uniquely altered. The most notable changes in relative temporal lobe size and proportions saw a decrease in relative temporal lobe size and proportions in the genus Pan compared to other extant great apes and fossil hominins while there was a relative increase in the temporal lobe width and length in Australopithecus-Paranthropus clade compared to the genus Homo and other extant great apes including modern humans. Discussion We do not find support for the social brain, environmental or functional craniology hypotheses alone but think it prudent to consider the implications of cerebral reorganization between the temporal lobes and other regions of the brain within the context of these hypotheses and with future investigation is warranted.

Three-dimensional Analysis of Presurgical Nasoalveolar Molding Outcomes in Patients with Unilateral Cleft Lip and Palate: A Preliminary Study Using LED Surface Scanning Technology.

ObjectivesPresurgical infant orthopedic appliances, such as presurgical nasoalveolar molding (PNAM) devices, are used to attain optimal conditions for primary repair of the lip and nose (PRoLN) in patients with cleft lip. We aimed to analyze the three-dimensional (3D) outcomes of PNAM using an LED surface scanner. MethodsFifteen patients with unilateral cleft lip and palate (CLP) were included in this study and treated using a PNAM device. The patients’ faces were digitized pre- and post-PNAM using an Artec Space Spider scanner, and the scanned data were analyzed using 3D software (Geomagic Control X and ANSYS SpaceClaim). The columellar angle (CA), nostril curvature (NC) on the affected (NC_A) and unaffected (NC_U) sides, gap of cleft lip (GCL), alar width (AW), and nasal tip angle (NTA) were measured. ResultsCA increased significantly by 10.00° and NC_A by 0.030 mm-1 (p<0.001). GCL decreased by 4.98 mm2 on average and NC_U by 0.015 mm-1 (p=0.029 and 0.046, respectively). AW also decreased by approximately 1.22 mm pre- and post-treatment (p=0.002), and NTA, which shows a lateral profile, decreased by approximately 3.32° (p=0.002). ConclusionsThis study confirmed the orthopedic benefits of PNAMd treatment through 3D analysis using an LED surface scanner. Further studies involving a larger number of participants are warranted to study the effects of PNAM and analyze longitudinal changes in patients with CLP. Clinical SignificanceThis study shows that PNAM effectively corrects columellar deviation and nostril shape in patients with unilateral cleft lip, with 3D scanners enhancing primary lip and nose repair outcomes.

Modeling wear of different surface layers of agricultural tools in soil abrasive mass

Uniform quantitative measures have not been developed to identify the areas with the greatest intensification of tool wear. Therefore, this paper developed original approach to assess the wear of cutting elements by identifying the intensity of local volumetric wear. A tribological test was conducted under natural operating conditions in two soil types: sandy loam and loam. The specimens were mounted on a 9-tined cultivator with spring tines aggregated with an agricultural tractor. A method was developed to discretise the local volume changes of a component using surface scanning models developed from 3D scanning. The notion of the rake angle has been defined, allowing the assessment of changes in the shape of the element during wear. The practical usefulness of the developed method was verified on three types of surface layers, i.e. 38GSA steel, ELHARD70 deposited on it, and a G10 layer of carbide. For each of the tested variants, local volumetric consumption was defined based on the identification of the wear grid. These should be the basis for developing the technology of surface strengthening of elements. The work confirmed the complexity of the process of wear of working elements in natural soil conditions, presented in many research works. A different course of wear of the 38GSA steel and the Elhard 70 weld layer was shown, depending on the type of treated soil. In the case of samples made of G10 cemented carbide, the largest volumetric wear for heavy soil occurred outside the place of reinforcement application. The highest volumetric wear in the front zone was recorded for 38GSA steel in heavy soil. Wear results from the modelling are similar to those obtained from the experiment under natural conditions in light soil using the nominal model (CAD) and the actual friction surface.

A perspective on Varian edge live view calibration

Modern radiotherapy relies heavily on precise, real-time monitoring of both the patient and the linear accelerator (linac) components. However, current commercial surface scanning systems often overlook the monitoring of linac components, such as the gantry, collimator, and couch, focusing solely on the patient. This study aims to address this issue by developing a comprehensive understanding of the Varian TrueBeam Edge system using a live view calibration method. This method ensures accurate alignment between the machine's 3D model and live camera images, improving the precision and reliability of radiotherapy treatments.

Microsurgical Anatomy of the Common Tendinous Ring and Its Surgical Implications

BACKGROUND AND OBJECTIVES: The common tendinous ring (CTR), also known as the common annular tendon or annulus of Zinn, is a critical anatomic structure located at the convergence of the orbital apex, superior orbital fissure (SOF), optic canal, and the anterior aspect of the lateral sellar compartment. It plays a vital role in both neurosurgical and neuro-ophthalmological interventions. The aim of this study was to delineate the complex 3-dimensional (3D) topography of the CTR and explore its implications for surgical procedures. METHODS: Ten formalin-fixed skull base specimens from adult Chinese cadavers were meticulously dissected to investigate the morphology of the CTR, focusing particularly on its relationship with the 4 extraocular rectus tendons, the optic strut, the SOF, and the optic canal. Additional skull base specimens were subjected to 3D surface scanning, computed tomography, and histopathological examinations to deepen our understanding of the CTR's structural complexities. RESULTS: The CTR establishes a spatial, 3D tendinous assembly, encompassing 4 rectus tendons, 2 tendinous connections, and a singular common tendinous root. These components interlink to form a distinctive dual-ring configuration, featuring the optic foramen and the oculomotor foramen. The posterior part of the superior rectus tendon demarcates the common boundary between these 2 foramina. The oculomotor foramen itself serves as the central sector of the SOF. Precise incisions of the medial and lateral tendinous connections and fusions are essential for safely opening the CTR. CONCLUSION: The structural composition, interconnections, and dual-ring configuration of the CTR are crucial for precise and safe surgery of orbital apex and adjacent regions.

Field investigation of the feasibility of MICP for Mitigating Natural Rainfall-Induced erosion in gravelly clay slope

Rainfall-induced erosion on slopes is a prevalent natural process leading to soil loss. One promising application of microbially induced carbonate precipitation (MICP) is to mitigate rainfall-induced erosion. Conducting field tests is an essential step to verify and improve its performance. In the current work, field tests were conducted to assess the feasibility of using MICP to mitigate rainfall-induced erosion on a gravelly clay slope in Longyan, Fujian, China. A temporary laboratory was set up to cultivate bacteria, and a non-sterilizing method was employed to prepare large volumes of bacterial suspensions in a single batch. Slopes were treated by spraying solutions onto their surfaces. The amount of discharged soils and 3D surface scanning results were used for evaluating the erosion intensity of the slopes. The results demonstrated that the method could effectively mitigate the surface erosion caused by natural rainfall and prevent erosion-induced collapse. Notably, approximately one year after the treatment, the grass had started to grow on the heavily cemented slope, indicating that the MICP method is both effective and eco-friendly for soil stabilization method. However, further improvements are needed to enhance the uniformity and long-term durability of the MICP treatment.

Sewage sludge ash as filler in asphalt mastic: Low-temperature towards high-temperature performance

The sewage sludge ash (SSA) is characterized as a by-product used in the pavement industry to mitigate the environmental risks, enhance the landfill capacity and conserve the non-renewable resources. This research utilized SSA as a filler substitute in asphalt mastic, and its performance-based properties from low towards high temperatures were evaluated. For this goal, the physical and chemical properties of SSA and rock powder (used as base filler) were determined using BET surface area measurement, X-ray fluorescence, and scanning electron microscopy tests. The performance of base and SSA-modified asphalt mastics in four filler/bitumen weight ratios (0.6, 0.8, 1, and 1.2) was investigated using dynamic shear rheometer, multiple stress creep and recovery, linear amplitude sweep, and bending beam rheometer tests across a wide temperature range. The results indicate that SSA has a higher specific surface area, better bitumen absorption, and lower density compared to the base filler. Additionally, unlike the acidic nature of the base filler, SSA possesses strong basic properties, leading to a stronger chemical bond with bitumen and enhanced resistance to aging, particularly at high temperatures. Moreover, the lower density of SSA escalates the amount of filler per unit volume of the SSA-modified asphalt mastic, leading to the increased stiffness and elasticity. This trend improves resistance to permanent deformation and cracking at medium temperatures, although it has an adverse effect on performance of mastic at low temperatures. Moreover, due to the improved performance of SSA-modified asphalt mastic, this study can contribute to the sustainable development of the pavement industry.

IMPACT OF THE WALL-PARALLEL LENGTH SCALE OF ROUGHNESS ON THE DEVELOPMENT OF TURBULENT BOUNDARY LAYERS IN TURBINES

Abstract The detrimental effects of surface roughness on turbulent boundary layers in turbines, and compressors, are well known. Prediction can be problematic, especially for surfaces which are not similar to sand grains. Several publications have proposed additional parameters, slope, skew, etc. to augment a wallnormal measure. Here, we introduce a new roughness parameter, the mean separation, which explicitly measures the average separation between each local minima and its closest local maxima. Scans of turbine blade surfaces have mean separations which are up to six times that of the sand grain surface with the same wallnormal measure. High resolution, &amp;lt; 0.1 μm, area scanning tools and 3-D printing techniques have enabled the development of a “Scan-Scale-Print-Measure” methodology. A surface scan is scaled-up, 3-D printed, applied to a flat-plate and then the turbulent boundary layer is measured. The methodology has enabled parametric studies where the mean separation was increased whilst keeping either the wallnormal or the feature size constant. Both studies demonstrated a surface roughness-loss that was strongly dependent on the mean separation and different to a sand grain correlation. It also showed that for large mean separations the roughness-loss decreased to zero. A study into pits-and-peaks showed that, at the same wallnormal, peaks generated a roughness-loss twice that of pits. An engine representative surface produced only half the roughness-loss that would be attributed to a sand grain surface. The roughness loss was estimated within 5% using the parametric studies.

Effect of annealing on microstructure and corrosion resistance of W+in-situ TiC enhanced CoCrFeNiSi0.2 high entropy alloy coatings by laser cladding

The W+in-situ TiC reinforced CoCrFeNiSi0.2 high-entropy alloy coating (The alloy coating) was prepared using laser cladding, and the effect of different annealing temperatures on the microstructure and corrosion resistance of the alloy coating was studied. The results indicated that annealing induces changes in the proportion of FCC and BCC phases in the coating matrix as well as the shape of carbides in the coating. Potentiodynamic polarization and electrochemical impedance experiments in 3.5 wt% NaCl solution revealed that the coating annealed at 1100°C exhibits the best corrosion resistance due to the presence of a single FCC phase and the formation of spherical carbides. Surface morphologies after corrosion showed deeper pits on the coating annealed at 700°C, which exhibited the poorest corrosion resistance due to the combined effects of galvanic and occluded cell corrosion. Potentiodynamic polarization curves demonstrated the presence of passive films on the surfaces of all four samples, and surface scan results after corrosion suggest that the passive film on the coating surface mainly consists of Ti- and W-containing oxides.

Evaluation of the remediation effect of solid waste synergistic materials on heavy metal-contaminated soil

In this study, we investigated the effect of various solid waste materials such as fly ash (CFA), steel slag, and kaolinite on the simultaneous stabilization of As, Cd, Cu, Pb, and Zn in the soil of abandoned lead-smelting slag plant. Synchronous stabilization of HMs in highly contaminated soils was achieved using the application of 29 % formulated material Fe/Ca-Si based co-remediation material (FCS) with a ratio of FeSO4 + CFA + kaolinite of 14 %: 12 %: 3 %, the bioavailable content of HMs As, Cd, Cu, Pb, and Zn was reduced by 71.55 %, 49.62 %, 91.35 %, 61.37 %, and 41.45 %, respectively, and had little effect on the pH of the soil. Leaching toxicity analyses guaranteed the safety of restorative materials. The results of the field validation experiments showed that the FCS was able to effectively reduce the content of HMs in plant rhizomes and stabilized with maintenance time. European Community Bureau of Reference (BCR) and Wenzel sequential extraction procedures showed that FCS promoted the transformation of HMs in soil from unstable to stable phases. The analysis of soil microbial community diversity by FCS showed that it created a favorable soil living environment for microorganisms and was conducive to the stabilization of HMs. SEM, Surface scanning analysis, XRD, and FTIR results showed that FCS reacts with HMs to form a stable phase. Cost analysis showed that it has certain economic benefits. In conclusion, FCS is feasible for soil remediation in lead-smelting slag-polluted areas and also provides a way for high value-added utilization of solid waste.

Immersive Photorealistic Three-Dimensional Neurosurgical Anatomy of the Cerebral Arteries: A Photogrammetry-Based Anatomic Study.

Neurosurgeons need a profound knowledge of the surgical anatomy of the cerebral arteries to safely treat patients. This is a challenge because of numerous branches, segments, and tortuosity of the main blood vessels that supply the brain. The objective of this study was to create high-quality three-dimensional (3D) anatomic photorealistic models based on dissections of the brain arterial anatomy and to incorporate this data into a virtual reality (VR) environment. Two formaldehyde-fixed heads were used. The vessels were injected with radiopaque material and colored silicone and latex. Before the dissections, the specimens were computed tomography scanned. Stratigraphical anatomic dissection of the neck and brain was performed to present the relevant vascular anatomy. A simplified surface scanning method using a mobile phone-based photogrammetry application was used, and the data were incorporated into a VR 3D modeling software for post-processing and presentation. Fifteen detailed layered photorealistic and two computed tomography angiography-based 3D models were generated. The models allow manipulation in VR environment with sufficient photographic detail to present the structures of interest. Topographical relevant anatomic structures and landmarks were annotated and uploaded for web-viewing and in VR. Despite that the VR application is a dedicated 3D modeling platform, it provided all necessary tools to be suitable for self-VR study and multiplayer scenarios with several participants in one immersive environment. Cerebral vascular anatomy presented with photogrammetry surface scanning method allows sufficient detail to present individual vessel's course and even small perforating arteries in photorealistic 3D models. These features, including VR visualization, provide new teaching prospects. The whole study was done with simplified algorithms and free or open-source software platforms allowing creation of 3D databases especially useful in cases with limited body donor-based dissection training availability.

The Ediacara Biota of the Wood Canyon formation: Latest Precambrian macrofossils and sedimentary structures from the southern Great Basin

Fossils of the Ediacara Biota preserve the oldest indisputable evidence of macroscopic animal life. Although new finds continue to expand the near global distribution of such organisms, relatively abundant and diverse populations of these primarily soft-bodied taxa are known from just a handful of localities. More commonly, reports of late Precambrian fossils consist of comparably lower diversity assemblages (less than ∼10 genera), with a limited number of total specimens (less than ∼1000 reported macrofossils over a broad geographic area). Determining the factors responsible for such differences can help to fully appreciate preserved patterns of Ediacaran biodiversity.The lower member of the Wood Canyon Formation in the Death Valley region of the southern Great Basin, USA contains fossils of the Ediacara Biota. Here we redescribe previously recovered specimens based on new data from 3D surface scans and report several new finds from this unit. Although this allows the addition of two Ediacara taxa (Pteridinium and Charnia?) to the known diversity in the region, most potential macrofauna consist of amorphous, irregular forms with minimal preserved structures wherein an abiotic origin cannot be ruled out. A combination of factors – including variable taphonomic conditions, difficulty of identification and an original depauperate community – contribute to the observed diversity of this assemblage. Many biases that impact this record are either common (e.g., age, preservation requirements, difficulty of recognition) or comparable (e.g., paleoenvironmental and taphonomic controls) to known occurrences of Ediacara macrofauana. Thus, we argue this fossil record at least partially reflects the original community composition, suggesting that fossils of the lower Wood Canyon record a signal of relatively low diversity of the Ediacara Biota in the lead up to the Ediacaran-Cambrian boundary.

Virtual skeleton and body mass for revealing the life strategies of Sinosaurus

ABSTRACT Sinosaurus from the Lufeng Basin, Yunnan Province, is the largest and most famous predatory dinosaur from the Early Jurassic in China. Although a number of specimens have been discovered in the past, no studies to date have focused exclusively on complete skeletal articulation, estimation of mass, or life strategies. Based on surface scans of a new well-preserved specimen, a digital skeleton of Sinosaurus was produced in two poses: osteologically neutral pose and extended pose. We estimated the possible living body mass by three volumetric-density methods, advocating the use of the expanding convex hulls method of 849.6 kg with the COM (0.41, 1.31, 0.00) as a more reliable result, which is greater than the mass of traditional regression formulas. The body shape and skull/body length suggested that Sinosaurus was a great runner, and this, combined with the weak crests and large forelimbs, further led to the conclusion that both the head and forelimbs were required to prey. This finding is also consistent with the finding that Sinosaurus has a moderate ability to metabolise body heat in the skin, as reflected by the surface/volume ratio.

Fabrication of micropatterned PCL-collagen nanofibrous scaffold for cellular confinement induced early osteogenesis

The intricate interaction of the scaffold's architecture/geometry and with the cells is essential for tissue engineering and regenerative medicine. Cells sense their surrounding dynamic cues such as biophysical, biomechanical, and biochemical, and respond to them differently. Numerous studies have recently explored and reported the effect of contact guidance by culturing various types of cells on different types of micropatterned substrates such as microgrooves, geometric (square and triangle) micropattern, microstrips, micropatterned nanofibers. Amongst all of these micropatterned polymeric substrates; electrospun nanofibers have been regarded as a suitable substrate as it mimics the native ECM architectures. Therefore, in the present study; stencil-assisted electrospun Grid-lined micropatterned PCL-Collagen nanofibers (GLMPCnfs) were fabricated and its influence on the alignment and differentiation of pre-osteoblast cells (MC3T3-E1) was investigated. The randomly orientated Non-patterned PCL-Collagen nanofibers (NPPCnfs) were used as control. The patterns were characterized for their geometrical features such as area and thickness of deposition using surface profiler and scanning electron microscopy. A 61 % decrease in the overall area of GLMPCnfs as compared to the stencil area demonstrated the potential of electrofocusing phenomenon in the process of patterning electrospun nanofibers into various micron-scale structures. The MC3T3-E1 cells were confined and aligned in the direction of GLMPCnfs as confirmed by a high cellular aspect ratio (AR = 5.41), lower cellular shape index (CSI = 0.243), and cytoskeletal reorganization assessed through the F-actin filament immunocytochemistry (ICC) imaging. The aligned cells along the GLMPCnfs exhibited elevated alkaline phosphatase activity and enhanced mineralization. Furthermore, the gene expression profiling revealed upregulation of key osteogenic markers, such as ALP, OCN, OPN, COL1A1, and osteocyte markers DMP1, and SOST. Consequently, the research highlights the impact of GLMPCnfs on the cellular behaviour that results to the pre-osteoblast differentiation and the potential for stimulant-free early osteogenesis. These results offer an extensive understanding and mechanistic insight into how scaffold topography can be modified to influence cellular responses for effective bone regeneration strategies.

Bacterial reduction and temperature increase of titanium dental implant models treated with a 445 nm diode laser: an in vitro study

In this in vitro study, the use of a 445 nm diode laser was investigated for the decontamination of titanium dental implants. Different irradiation protocols and the effect of repetitive laser irradiation on temperature increase and decontamination efficacy were evaluated on titanium implant models. An automated setup was developed to realize a scanning procedure for a full surface irradiation to recapitulate a clinical treatment. Three irradiation parameter sets A (continuous wave, power 0.8 W, duty cycle (DC) 100%, and 5 s), B (pulsed mode, DC 50%, power 1.0 W, and 10 s), and C (pulsed mode, DC 10%, power 3.0 W, and 20 s) were used to treat the rods for up to ten consecutive scans. The resulting temperature increase was measured by a thermal imaging camera and the decontamination efficacy of the procedures was evaluated against Escherichia coli and Staphylococcus aureus, and correlated with the applied laser fluence. An implant’s temperature increase of 10 °C was set as the limit accepted in literature to avoid thermal damage to the surrounding tissue in vivo. Repeated irradiation of the specimens resulted in a steady increase in temperature. Parameter sets A and B caused a temperature increase of 11.27 ± 0.81 °C and 9.90 ± 0.37 °C after five consecutive laser scans, respectively, while parameter set C resulted in a temperature increase of only 8.20 ± 0.53 °C after ten surface scans. The microbiological study showed that all irradiation parameter sets achieved a complete bacterial reduction (99.9999% or 6-log10) after ten consecutive scans, however only parameter set C did not exceed the temperature threshold. A 445 nm diode laser can be used to decontaminate dental titanium rods, and repeated laser irradiation of the contaminated areas increases the antimicrobial effect of the treatment; however, the correct choice of parameters is needed to provide adequate laser fluence while preventing an implant’s temperature increase that could cause damage to the surrounding tissue.

Stabilization of Fish Protein-Based Adhesive by Reduction of Its Hygroscopicity.

Protein-based fish adhesives have historically been used in various bonding applications; however, due to the protein's high affinity for water absorption, these adhesives become destabilized in high-moisture environments, resulting in reduced bondline strength and early failure. This limitation makes them unsuitable for industrial applications with higher demands. To address this issue, water-insoluble raw powder materials such as iron, copper, or zeolite were incorporated into natural fish adhesives. In this study, the hygroscopicity, dry matter content, thermal analysis (TGA/DSC), FT-IR spectroscopy, surface tension measurements, vapour permeability, and scanning electron microscope (SEM) of the modified adhesives were determined. In addition, the bonding properties of the modified adhesives were evaluated by the tensile shear strength of the lap joints, and mould growth was visually inspected. The resulting modified protein-based adhesives demonstrated improved stability in high humidity environments. Enhancing the hygroscopic properties of protein-based fish adhesives has the potential to unlock new opportunities and applications, providing a healthier and more environmentally sustainable alternative to petroleum-based adhesives.