Suppression Of Pythium Research Articles

Interaction of Brassicaceae Seed Meal Soil Amendment and Apple Rootstock Genotype on Microbiome Structure and Replant Disease Suppression.

Preplant soil application of a Brassica juncea-Sinapis alba seed meal formulation (SM) at a rate of 6.6 t ha-1 alters composition of the orchard soil microbiome in a manner that yields sustainable long-term suppression of soilborne pathogens in apple production systems. However, the cost of SM amendment has hindered the adoption of this tactic to manage apple replant disease in commercial orchards. Greenhouse trials were conducted to assess the effect of reduced SM application rates in concert with apple rootstock genotype on structure of the rhizosphere microbiome and associated disease control outcomes. At all application rates assessed, SM treatment increased tree growth and reduced disease development relative to the control. In general, total tree biomass and leader shoot length were similar in soils treated with SM at 4.4 or 6.6 t ha-1 regardless of rootstock genotype. Equivalent increase in tree biomass when cultivated in soil treated at the lowest and highest SM amendment rate was attained when used in conjunction with G.41 or G.210 apple rootstocks. Suppression of Pythium spp. or Pratylenchus penetrans root densities was similar at all SM application rates. When cultivated in nontreated replant orchard soil, Geneva rootstocks (G.41 and G.210) exhibited lower levels of Pythium spp. and P. penetrans root colonization relative to Malling rootstocks (M.9 and MM.106). For a given rootstock, structure of the rhizosphere microbiome was similar in soils treated with SM at 4.4 and 6.6 t ha-1. G.41 and G.210 rootstocks but not M.9 or MM.106 cultivated in soil treated with SM at 2.2 t ha-1 possessed a rhizosphere bacterial community structure that differed significantly from the control. Findings indicate that effective control of apple replant disease may be attained at lower SM amendment rates than employed previously, with lower effective rates possible when integrated with tolerant rootstock genotypes such as G.41 or G.210.

Suppression of Pythium and Phytophthora Damping-Off of Rooibos by Compost and a Combination of Compost and Nonpathogenic Pythium Taxa.

Pathogenic oomycetes, including Phytophthora cinnamomi and several Pythium spp. (Pythium irregulare, P. mamillatum, P. myriotylum, and P. pyrilobum), cause serious damping-off problems in rooibos (Aspalathus linearis) nurseries. The management of these pathogens in organic nurseries is problematic, because phenylamide fungicides may not be used. Compost, or compost in combination with Pythium taxa that are nonpathogenic to rooibos (P. acanthicum, P. cederbergense, and Pythium RB II), were investigated as alternative management options. Compost was able to suppress damping-off caused by several oomycete isolates but there was within- and between-species variation among the 30 evaluated isolates. This phenomenon was observed using two compost batches (A and B) sourced from independent suppliers. Compost B significantly reduced damping-off caused by 60% of the isolates, whereas compost A controlled only 37% of the isolates. The pathogens that were more readily controlled by both composts included P. mamillatum and P. pyrilobum, whereas the composts were ineffective at suppressing damping-off caused by >62% of P. irregulare and >50% of P. myriotylum isolates. Based on the evaluation of one Phytophthora cinnamomi isolate, this pathogen may also be controlled by compost. Neither of the composts as a stand-alone treatment could suppress damping-off caused by a combination of pathogenic species (P. cinnamomi, Pythium irregulare, P. mamillatum, P. myriotylum, and P. pyrilobum). However, damping-off was significantly reduced when nonpathogenic Pythium taxa (P. acanthicum, P. cederbergense, and Pythium RB II) were combined with the composts. Similarly, damping-off caused by a P. irregulare isolate that was not suppressed by either of the composts alone was significantly suppressed when the two composts were inoculated with the nonpathogenic Pythium taxa.

Pyoluteorin production byPseudomonas fluorescens strain CHA0 is involved in the suppression ofPythium damping-off of cress but not of cucumber

Pseudomonas fluorescens strain CHA0 is an effective biocontrol agent of various soilborne pathogens. It controls damping-off or root rot caused byPythium ultimum on cucumber, wheat and cress. Strain CHA0 synthesizes several antibiotic metabolites such as hydrogen cyanide, 2,4-diacetylphloroglucinol, and pyoluteorin. The role of pyoluteorin in the suppression of damping-off was investigated. Two Tn5 mutants (CHA660 and CHA661) of strain CHA0 were isolated which had lost the capacity to produce pyoluteorin but still produced 2,4-diacteylphloroglucinol and HCN. These mutants still inhibitedP. ultimum on malt agar (which favours the production of 2,4-diacetylphloroglucinol) but had partially lost the ability to inhibit this pathogen on King's B agar (which favours the production of pyoluteorin). The two pyoluteorin-negative mutants showed a reduced capacity to suppress damping-off of cress caused byP. ultimum but were as effective in the protection of cucumber against this pathogen as the wild-type strain.

Composting of agricultural wastes for their use as container media: effect of heat treatments on suppression of Pythium aphanidermatum and microbial activities in substrates containing compost

Composted separated manure (CSM) and composted grape marc (CGM) were found to suppress Pythium damping off. This effect was negated by autoclaving the medium, but was restored when compost that had not been autoclaved was mixed with the sterile one. Heat treatment (55°C for 2 h) did not affect suppression even after two growth cycles: in fact, suppression of Pythium in compost and peat mixtures was improved by heating. It is suggested that microorganisms in CSM are more thermostable than peat microflora. Fungal and bacterial counts as well as measurements of biological activities were performed. The CSM microflora was more active than the peat population in terms of fluorescein diacetate hydrolysis and CO 2 evolution. Both activities were less sensitive to 55°C heat treatments in CSM than in peat, but as sensitive as in peat to 80 and 100°C heat treatments. The effect of incubation on the activities was studied and different patterns were observed for CSM and peat. The role of microbial activity in suppression of Pythium is discussed.

Effects of Municipal Sludge Compost Curing Time on Suppression of Pythium and Rhizoctonia Diseases of Ornamental Plants

Effects of Municipal Sludge Compost Curing Time on Suppression of Pythium and Rhizoctonia Diseases of Ornamental Plants