Pest Suppression Research Articles

Linalool fumigation improves mating competitiveness of males for population suppression of the global fruit pest Cydia pomonella

AbstractBACKGROUNDThe implementation of sterile insect technique (SIT) has proven effective in the area‐wide suppression of several significant agricultural and sanitary pests by using traditional cobalt‐60 (60Co‐γ) as a radiation source. Recently, X‐ray has been validated as a feasible alternative to 60Co‐γ radiation sources. Nonetheless, higher doses of X‐ray irradiation led to insect sterility but diminish mating competitiveness, thereby impacting the effectiveness of SIT applications.RESULTSIn this study, we assessed the impact of various X‐ray irradiation doses (ranging from 0 to 366 Gy) on the fecundity, fertility, and mating competitiveness of Cydia pomonella, a globally invasive fruit pest. Results demonstrated that the sterility rate of irradiated males increased with dose up to 200 Gy, then stabilized. Exposure to 200 Gy reduced male mating competitiveness, with competitiveness index (CI) values of 0.17 in the laboratory and 0.096 in the orchard. This decline is likely linked to the decreased expression of genes associated with sex pheromones recognition, such as CpomOR3a, CpomOR3b, and CpomOR5, post‐irradiation. Fumigation of linalool at varying concentrations (70, 83, and 96 μL/m3) enhanced mating competitiveness of males, particularly at moderate levels, possibly by restoring pheromones recognition. Implementation of repeated releases of sterilized males on a pilot scale led to a notable reduction in the population of C. pomonella in the field.CONCLUSIONThese findings indicate that fumigation with plant volatiles has the potential to mitigate male sterility induced by X‐ray irradiation, offering a promising approach to enhance the efficacy of SIT applications for the control of C. pomonella. © 2024 Society of Chemical Industry.

Comparative analysis of active period and oxidative stress of DDVP and ginger (Zingiber officinale) oil on Indian meal moth (Plodia interpunctella Hübner) infesting maize grain

BackgroundIndian meal moth (Plodia interpunctella) is a significant pest infesting stored grains, particularly maize. Over time, synthetic insecticides have been employed to control insect. The residual effects posed on non-target organisms have called for replacement of synthetic insecticides with botanicals. This study therefore aimed at comparing the insecticidal consistency and oxidative stress invoked by dichlorvos (DDVP) and the oil extract of ginger (Zingiber officinale) on Indian meal moth infesting maize. Disinfested maize grains were treated with DDVP and ginger oil extract separately. Adults P. interpunctella were introduced to the treated grains daily using complete replacement method. The percentage mortality was calculated daily for 10 d. Furthermore, the oxidative stress caused by DDVP and ginger oil extract on the moth was evaluated by measuring the level of some oxidative stress biomarkers such as glutathione S-transferase, superoxide dismutase, glutathione peroxidase (GPx), and catalase (CAT) activity in the exposed insects.ResultsPreliminary results indicated that both DDVP and ginger oil extract exhibited insecticidal properties against Indian meal moth infesting maize. However, the insecticidal (active) period of ginger oil extract was found to be longer than that of DDVP. Nevertheless, DDVP provoked greater oxidative stress in the exposed moth.ConclusionsGinger oil extract and DDVP show potential for controlling Indian meal moth infestations in stored maize. Yet, ginger oil offers a longer-lasting effect on pest suppression and control. Consequently, it could be a replacement or synergistic insecticide with DDVP to provide ecofriendly insecticide application.

Invisible Allies: The Role of Endophytic Entomopathogenic Microbes in Insect Pest Control

Endophytic entomopathogenic microbes, including both bacteria and fungi, hold significant promise as biocontrol agents in sustainable pest management. While the biocontrol potential of entomopathogens are well-established, their role as mutualistic endophytes within plant tissues offers a relatively new avenue for enhancing pest suppression. The ability of these microbes to colonize plant tissues allows them to serve as internal defense agents against insect pests, while simultaneously promoting plant health and resilience to environmental stressors. Here, we explore the growing body of knowledge surrounding the use of endophytic entomopathogenic bacteria and fungi in pest management. These microbes contribute to pest control through direct effects on insect populations, such as mortality and feeding inhibition, as well as indirect plant-mediated mechanisms that enhance plant defenses. Additionally, endophytic entomopathogens provide a range of benefits to their plant hosts, including growth promotion, nutrient acquisition, and protection against pathogens. Despite the clear advantages of using endophytic entomopathogens, there remain key challenges to their widespread implementation. Environmental factors can influence their persistence and efficacy in the field, and their relatively slow mode of action compared to chemical pesticides presents a hurdle for rapid adoption in commercial agriculture. Non-target effects and the development of effective formulations to ensure consistent colonization are also areas that require further investigation. Addressing these challenges through continued research into the complex interactions between endophytes, insects, plants, and their environments will be essential for advancing the integration of endophytic entomopathogenic microbes into sustainable pest management systems.

Insect Phototaxis Mechanisms Innovations in Pest Control Strategies and Applications

Phototaxis, the movement of insects toward or away from light, is a critical behavioral response that influences feeding, mating, and habitat selection. Understanding the mechanisms behind insect phototaxis can lead to the development of effective and environmentally sustainable pest control strategies and explores the biological foundations of phototaxis, including the role of insect visual systems, light sensitivity, and circadian rhythms. Recent advancements in phototaxis-based pest management, particularly the use of energy-efficient LED light traps, solar-powered traps, and wavelength-specific attractants, are also discussed. These innovations offer promising alternatives to chemical pesticides, reducing environmental impact and enhancing agricultural sustainability. The integration of phototaxis-based tools with other methods, such as biological control and habitat management, is highlighted as a way to improve pest suppression while preserving beneficial insect species. Challenges, including non-target effects, variability in insect responses, and potential light pollution, are also addressed. To emphasize the need for tailored light wavelengths, smart farming integration, and eco-friendly solutions that minimize unintended ecological consequences and underscores the potential of insect phototaxis to transform pest management, offering a comprehensive framework for more sustainable agricultural practices.

Modelling agricultural landscape complementation for natural pest control

Abstract Pest‐regulating natural enemies often require crop and non‐crop habitats to persist and be effective. These habitats offer hibernation sites, alternative prey or floral resources but can also be complementary in when they provide resources. The relative contribution of these different habitats to the performance of pest‐regulating insects is largely unknown, since it is difficult to study empirically. Landscape‐based population‐dynamical modelling can fill this research gap. In this study, we modelled the dynamics of predatory hoverflies (Diptera: Syrphinae) and their aphid prey in a collection of habitats. These hoverflies are dependent on different habitats for different reasons. While their larvae predate on aphids, adult hoverflies require floral resources. Resources are often segregated in different habitats. In addition, they are only temporarily available in any habitat type. In our model, hoverflies move between different habitats and select where to feed and where to produce off‐spring based on optimal foraging considerations. The model represents habitats common in arable landscapes and their temporal availability of resources is parameterized based on field observations. The model shows that effective pest suppression will only occur in the presence of at least three different habitats, including two crops that provide prey resources at different times of the year. A woody habitat with shrubs and trees provide aphid prey very early and late in the season, whereas an early crop and a late crop cover the period in between. In addition, these habitats need to provide floral resources in the same period as aphids, for example by flower strips in the margins of crop fields or blossoming trees or shrubs in the woody habitat. Under the model assumptions, the best pest suppression is obtained when the woody habitats take up 6% to 10% of the landscape. In addition to yearly population fluctuations, also bi‐ or multi‐yearly cycles may occur, depending on the composition of the landscape. Synthesis and applications. Our model shows when an agricultural landscape is ‘complete’ for natural enemies (and for effective pest control). For this, it needs to contain multiple habitats that supply floral and prey resources in different periods of the year.

Modeling the effects of trap crop attraction strength, patch dispersion, and biological control on pest suppression

Modeling the effects of trap crop attraction strength, patch dispersion, and biological control on pest suppression

Cover crops dismantle keystone ant/aphid mutualisms to enhance insect pest suppression and weed biocontrol

Abstract Cover crops are multifunctional tools that mitigate environmental impacts of agriculture, enhance resilience to weather extremes and suppress weeds and arthropod pests. Cover crops provide non‐crop food and habitat resources that attract natural enemies of pests, but their outcomes for pest management are less clear in regions where keystone mutualisms between red imported fire ants and aphids dominate. Here, we manipulate ant exclusion treatments and cover crop treatments (living mulches and terminated cover crops) that vary in food/habitat resources and examine responses of ants, aphids, other herbivores and predators in a cotton agroecosystem. Living mulches reduced both ants and aphids in the crop canopy by 97% and 93%, respectively, relative to bare soil treatments, and terminated cover crops reduced them as well by a lesser degree (~50%). Non‐aphid herbivores occurred in low densities system‐wide and increased in living mulches, whilst native predators had variable responses to cover crops and ant exclusion. Cover crops had no effect on prey removal in the crop canopy, but living mulches tripled rates of weed seed biocontrol, relative to bare soils. Cover crops elicited a shift in fire ant foraging from cotton foliage downward to the soil‐surface, preventing competitive exclusion by keystone ant/aphid mutualists that dominate crop monocultures. Cover crops altered the system‐wide impacts of fire ants: reducing ecosystem disservices (i.e. aphid tending) and enhancing ecosystem services (i.e. weed seed biocontrol). These results provide incentives for cover crop adoption as a regenerative practice in large‐scale commercial agriculture.

Banker Plant Efficacy to Boost Natural Predators for Management of Field Populations of Scirtothrips dorsalis Hood (Thysanoptera Thripidae) in Strawberries.

Since 2015, Scirtothrips dorsalis Hood has emerged as the main pest of strawberries in Florida. Given the limited management options, there is a recognized need to expand on the management strategies for this pest. Therefore, we explored the possibility of using banker plants to recruit naturally occurring predators of thrips into strawberry fields to suppress S. dorsalis. The study began in the 2021-2022 strawberry season where five banker plants were screened to determine which ones could consistently attract thrips predators by flowering throughout the strawberry season. Capsicum annum L. (ornamental pepper) and Lobularia maritima L. (sweet alyssum) were selected for further evaluation. In the 2022-2023 strawberry season, using a randomized complete block design we assessed the capability of these banker plants to attract thrips predators into the strawberry field. In addition, we examined how the banker plant distance from the strawberry plants influenced the S. dorsalis pest suppression. Our results showed that strawberries located within 3.7 m of ornamental pepper plants had less leaf damage from S. dorsalis compared with those farther away, which may result from the repellent effect of the ornamental peppers. Additionally, Geocoris spp. and Orius spp. were identified as the main thrips predators in the system, although in relatively low numbers. Therefore, these results highlight the potential of incorporating ornamental pepper as a banker plant in strawberry production. Additional applications of this research are explored below.

Evidence at the landscape level links high predator/pest ratios to biocontrol services against aphids

Compared with external environment interferences, the interaction between arthropod natural enemies and pests is the key inherent driver determining the strength of biocontrol services. However, the extent to which this effect can suppress pests is still inconclusive. We combined two complementary experiments to determine how variation in an assemblage of generalist arthropod predators modified the level of biocontrol services for suppression of a key aphid pest in cotton fields. Using generalized linear mixed effect models (GLMM) and path analysis, we clarified the causal relationship between predator/aphid ratios (PAR) and (1) the resulting biocontrol services index (BSI) and (2) the aphid population growth index (APGI) as seen in field predator exclusion trials. We also measured the effect of PAR on APGI in more commercial cotton fields. Our results, at landscape level, indicate that when PAR values increased one unit, BSI values improved 34.1 %, and reduced aphid population growth 28.3 % (the standardized effect coefficient in path analysis) during two weeks in field cage-exclusion trials. The effect of high predator/aphid ratios on reducing the aphid population growth rates was also significant in more commercial cotton fields, which were sampled over a longer time interval (4 weeks). Our study confirmed that there was a causal relationship between the natural enemy/pest ratio and the level of biological pest control services for predators and aphid population growth in crop fields. PAR values (predator/aphid ratios) can, therefore, be used to predict the level of biocontrol services in this context instead of relying on more labor-intensive cage exclusion studies.

Do Sex Ratio Distorting Microbes Inhibit the Evolution of Pesticide Resistance? An Experimental Test.

We are still largely reliant on pesticides for the suppression of arthropod pests which threaten human health and food production, but the recent rise of evolved resistance among important pest species has reduced pesticide efficacy. Despite this, our understanding of strategies that effectively limit the evolution of resistance remains weak. Male-killing sex ratio distorting microbes (SRDMs), such as Wolbachia and Spiroplasma, are common among arthropod species. Previous theoretical work has suggested that they could limit adaptive potential in two ways: first, because by distorting sex ratios they reduce the effective population size, and second, because infected females produce no male offspring which restricts gene flow. Here we present the results of a novel experiment in which we test the extent by which these two mechanisms limit the adaptive response of arthropods to pesticide. Using a fully factorial design, we manipulated the adult sex ratio of laboratory populations of Drosophila melanogaster, both in the presence and absence of SRDMs, and exposed these populations to six generations of pesticide poisoning. This design allows the effects of SRDMs on sex ratio and their effects on gene flow to be estimated separately. After six generations, individuals from populations with even sex ratios displayed a higher resistance to pesticide relative to individuals from female-biased populations. By contrast, we found no effect of the presence of SRDMs in host populations on pesticide resistance independent of sex ratio. In addition, males were more susceptible to pesticide than females-this was true of flies from both naïve and previously exposed populations. These findings provide the first empirical proof of concept that sex ratio distortion arising from SRDMs can limit adaptation to pesticides, but cast doubt on the theoretical effect of male-killers limiting adaptation by disrupting gene flow.

Remote sensing reveals the importance of seminatural habitat and irrigation on aphid biocontrol in arid agroecosystems

Abstract Remote sensing and spatial analyses are increasingly used to understand the role of seminatural habitats in biocontrol, but knowing how to best leverage these tools is a persistent challenge. In arid agroecosystems, irrigation often exaggerates the differences between crop fields and their interspaces. Weeds adjacent to irrigated fields are thus often some of the only persistent vegetation. Remote sensing may be essential to capture the effect of this fine‐scale variation on pest biocontrol. We examined the role of land‐cover in determining the degree of aphid pest pressure and biocontrol in irrigated alfalfa fields in the Great Basin Desert (Nevada, USA). We experimentally manipulated predator densities to determine true agents of aphid biocontrol in this system. We then assessed land‐cover and spatial scale using alternative methods to determine the approaches that best defined the combined direct and indirect effects of seminatural habitat on aphid pest suppression. A remote‐sensing approach revealed the importance of land‐cover to aphid biocontrol more clearly than conventional vegetation surveys. In particular, remote sensing revealed that weedy patches within alfalfa fields reduced aphid densities, whereas flood irrigation increased aphid densities. Our experiments confirmed that coccinellid beetles are important aphid biocontrol agents. Coccinellids were positively associated with weedy land‐cover that was spatially close to alfalfa fields, particularly in the spring. Other predator taxa were also associated with distinct landscape features, though these associations varied between spring and fall. Solution: Weedy seminatural habitat near alfalfa fields, occurring along field margins and the banks of irrigation ditches, was positively associated with the density of coccinellid beetles and associated aphid pest suppression. Remote sensing excelled at identifying these areas and their effects, highlighting its utility as a management tool. Preserving these weedy areas can enhance aphid biocontrol, but farmers must consider potential trade‐offs between the control of insect‐pests and weeds.

Predatory potential of Coccinella undecimpunctata L. (Coleoptera, Coccinellidae) on Aphis craccivora Koch. (Hemiptera, Aphididae) in Ladakh, India

Predatory potential of all instar larvae and adults of Coccinella undecimpunctata (Coleoptera, Coccinellidae), using Aphis craccivora Koch (Hemiptera, Aphididae) was assessed in laboratory settings. The study, conducted with varying aphid densities in rearing jars, showed a significant increase in prey consumption as aphid density rose. Furthermore, prey consumption heightened with larval development, with 4th instars devouring more aphids compared to earlier instars. Female adults exhibited greater aphid consumption than males. Deploying both 4th instar larvae and adults could enhance pest suppression.

The role of landscape context in shaping bat assemblages in African cacao plantations

Cacao production is mostly concentrated in Africa, with this continent exporting an impressive 68.4 % of the world's cacao. The increasing demand for cacao from the Global North has already led to massive deforestation in Ghana and Ivory Coast and cacao-driven deforestation is likely to continue changing landscapes in Sub-Saharan Africa. Bats are affected by these landscape changes due to their dependence on multiple resources spread at a large spatial scale. Although bats can save cacao farmers millions of euros through pest suppression, no study has investigated how landscape context affects bat communities in African cacao plantations. Here for the first time, we studied how abundance and richness of insectivorous, frugivorous and nectarivorous bats within cacao landscapes could be affected by cover type and the distance between these habitats and each cacao plantation. We sampled bats using mist-nets in 38 cacao plantations spread throughout southern Cameroon from 2017 to 2020. We found that guilds responded differently to the distance and amount of cover of each of the land cover types, with the scale of response being habitat-dependent. Overall, insectivorous bats were associated positively with high cover of natural habitats (e.g., tree cover, rangeland, and flooded vegetation), and negatively with nearby anthropogenic disturbance (e.g., logging and intensive agriculture). Frugivorous and nectarivorous bats were associated to the presence of natural habitats with water and of nearby anthropogenic habitats (e.g., human settlements, community forests and unpaved roads), probably due to the presence of more fruiting and flowering trees. Considering the associations found between the landscape metrics and bats, we propose three different conceptual designs to manage cacao landscapes: one for insectivores, one for frugivores/nectarivores and a third design that maximises the trade-offs between these three guilds. By safeguarding the diversity of these three guilds farmers can maintain pest suppression services within their plantations and guarantee healthy and long-lasting sustainable cacao landscapes through bats' pollination and seed dispersal.

Population suppression by release of insects carrying a dominant sterile homing gene drive targeting doublesex in Drosophila

CRISPR homing gene drives can suppress pest populations by targeting female fertility genes, converting wild-type alleles into drive alleles in the germline of drive heterozygotes. fsRIDL (female-specific Release of Insects carrying a Dominant Lethal) is a self-limiting population suppression strategy involving continual release of transgenic males carrying female lethal alleles. Here, we propose an improved pest suppression system called “Release of Insects carrying a Dominant-sterile Drive” (RIDD), combining performance characteristics of homing drive and fsRIDL. We construct a split RIDD system in Drosophila melanogaster by creating a 3-gRNA drive disrupting the doublesex female exon. Drive alleles bias their inheritance in males, while drive alleles and resistance alleles formed by end-joining cause dominant female sterility. Weekly releases of RIDD males progressively suppressed and eventually eliminated cage populations. Modeling shows that RIDD is substantially stronger than SIT and fsRIDL. RIDD is also self-limiting, potentially allowing targeted population suppression.

The Use of Bio-control Agents in Mulberry Pest Management: Successful Techniques and Important Issues

Since bio-control agents target specific pests without affecting the usefulness insects or/and damaging the soil and water resources, it plays a role in conservation of ecological balance in the environment. A variety of insect and non-insect pests, as well as hosts of parasites (fungi, insect, and mite parasites), occur in silkworm sericulture. This method does not in any way influence the sustainability of environment through pest control as required by sustainable agriculture. Since bio-control agents are known to be harmless to human beings and other organisms that may be of equal importance in the ecosystem such as pollinators, wildlife etc., they also have safety advantages. This is a big step forward to eradicating pests as compared to the chemical pesticides which have been known to affect the human lives and other wildlife. In the meantime, the bio-control agents may be cost effective in the long run. Because bio-control agents could offer continuous control of pests with minimal extra cost, it is possible, even when the outlay of obtaining and developing such organisms is more expensive than straightforward chemical applications. Further, the use of bio-control agents may reduce the reliance on traditional chemical pesticides and hence cost outlay and pest control costs and also reduce the probabilities of pesticide resistance. In the farming of mulberry, bio control agents are one the most important components of integrated pest management and have proved be an effective substitute to chemical control. Bio-control agents offer long-term pest suppression in comparison to chemical control methods, whose impacts tend to weaken after several generations of treatment. The continuous use of chemicals may result in pest resistance to the pesticides used. Spraying synthetic pesticides leads to the formation of pesticide residues on mulberry foliage, which affects food products and environmental health.

Potential biodiversity maps of Geadephaga (Coleoptera) from subantarctic forests: Relating diversity patterns and conservation hotspots with forest integrity

Abstract Potential biodiversity maps (PBMs) allow the identification of areas with different potential for conservation, to support political decisions about the management and protection of biodiversity. As these maps are seldom constructed for inconspicuous species, we proposed to develop PBMs for species belonging to the Geadephaga (Coleoptera), which is a group of beetles that contributes as predators for pest suppression and other ecosystem services in forest ecosystems. Given that human activities are reducing forest integrity, we consider that it is crucial to recognize how diversity patterns of Geadephaga are related to degraded forests. We developed these maps for the Geadephaga associated with subantarctic forests considering diversity measures of species richness, specificity, and rarity to establish spatial relationships between each diversity measure and different levels of forest integrity, and to identify potential hotspots and suggest conservation priorities. Results showed a latitudinal pattern of decrease in scores on richness and specificity from north to south, but a patchy pattern of species rarity across the region. Outcomes also show that areas with high scores of diversity measures are overlapped with degraded forest, and that hotspots have a low spatial overlap between them. In this work, we provide for the first time regional PBMs at a relatively high spatial resolution of three different diversity measures for Geadephaga that inhabit subantarctic forest. These maps constitute tools that allow not only to recognize potential diversity patterns of these insects, but also to offer valuable information to be used in conservation decision‐making.

Perennial cultivation of green pepper provides pest suppression, ecosystem development and high crop yield

Perennial cultivation (PC) provides economic benefits to farmers and environmental benefits to society such as soil protection. However, little is known about the effects of PC on soil pest occurrence. This 4-year study used overwintered green pepper (GP) and root-knot nematodes (RKN) as a model plant–soil pest system. We compared the levels of RKN, its antagonists, and other organisms in strip-shaped PC microplots to those of annual cultivation (AC) and no-tillage AC (NT) microplots. To promote RKN occurrence, we inoculated the ends of all microplots with RKN in the spring of year 3 of study. During autumn in years 3 and 4, we determined abundance of RKN and antagonistic animals, DNA operational taxonomical units (OTUs) counts of antagonistic microbes. The results showed that the rate of RKN occurrence was lower in PC and NT than in AC due to minimal soil movement, rather than to suppression by antagonists, although some antagonists such as collembolan and nematode-trapping fungi increased in PC. Moreover, plant biomass was higher in PC than in NT and AC during fruiting period due to persistence of the plant body over years.We also found signs of ecosystem development in PC, which is an essential background for pest suppression: the maturity index (a measure of disturbance) of the nematode community, fungal diversity, frequency of plant and animal symbionts/parasites were greater in PC than in NT or AC. These findings suggested that ecosystem development had commenced within the 4-year study period. We conclude that the long-lasting plant body and undisturbed soil environment are specific to PC, and could provide agronomical and ecological benefits.

Rice Bund Management by Filipino Farmers and Willingness to Adopt Ecological Engineering for Pest Suppression

Ecological engineering is defined as the design of ecosystems for the benefit of human society and the environment. In Asia, the ecological engineering of rice fields by establishing vegetation on bunds/levees for natural enemies has recently gained traction; however, its success depends on farmers’ willingness to implement changes. We surveyed 291 rice farmers in four regions of the Philippines to assess their bund management practices and willingness to establish bund vegetation that restores rice regulatory ecosystem services for pest management. During pre- and post-open field day (OFD) interviews, we assessed farmers’ perceptions of ecological engineering practices and sought their advice concerning bund vegetation. Over 60% of the farmers grew crops or allowed weeds on their bunds. Vegetables were grown as a source of extra food or income, and flowers were grown for pest management. Among the remaining farmers, their willingness to try ecological engineering increased from 36 to 74% after the OFDs. Furthermore, after the OFDs, willing farmers increasingly (from 2.6 to 15%) cited pest management as a reason to grow vegetables on bunds, and farmers almost exclusively focused on growing vegetables rather than flowers to adapt the system. While 46.5% of farmers who grew vegetables on their bunds applied insecticides, only ca 20% indicated that they would do so after the OFDs, if needed. Farmers had differing opinions on how vegetables would be incorporated into their rice farms. This range of options could be encouraged wherever farmers recognize the potential harm from pesticides for biodiversity and the restoration value of a diversified farm habitat.

Endophytic entomopathogenic fungi as biological control agents of insect pests.

Entomopathogenic fungi capable of establishing mutualistic endophytic relationships with plants have a tremendous potential as biocontrol agents of insect pests. While fungi have long played an important and highly effective role in pest suppression, the utility of endophytic entomopathogenic fungi in pest management is a relatively new and emerging topic of biocontrol. Here we discuss the relevance of endophytic fungi to plant health in general, synthesize the current knowledge of the effectiveness of endophytic entomopathogenic fungi against diverse insect pests, discuss the indirect plant-mediated effects of endophytic entomopathogenic fungi on arthropods, and describe the diverse benefits of endophytic fungi to plants that are likely to affect herbivores and plant pathogens as well. Lastly, we consider major challenges to incorporating endophytic entomopathogenic fungi in biocontrol, such as their non-target effects and field efficacy, which can be variable and influenced by environmental factors. Continued research on endophyte-insect-plant-environment interactions is critical to advancing our knowledge of these fungi as a sustainable pest management tactic. © 2024 The Author(s). Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

Predation efficacy of Chrysoperla carnea on two economically important stink bugs

Larvae of the green lacewing Chrysoperla carnea (Stephens) (Neuroptera: Chrysopidae) are generalist predators feeding on a range of pests in agroecosystems. Little is known about the suitability of stink bugs as prey for lacewing larvae. In the present laboratory study, the predatory efficacy of C. carnea on two economically important stink bugs, Halyomorpha halys (Stål) and Nezara viridula (L.) (Hemiptera: Pentatomidae), was evaluated at three constant temperatures (16, 21, and 26 °C) in different arenas (10-cm diameter dishes with bean pod slices or caged small bean plants). We found that second and third instars of C. carnea readily prey on the first instars of both stink bug species. However, only third instars of C. carnea could successfully kill and feed on second instars of either pentatomid. The complexity of the arena, as well as the life stage of the prey and predator influenced the predation efficacy of C. carnea on stink bug nymphs, whereas temperature did not. The observed predation rates on the early instars of both stink bugs suggest that augmentative releases of C. carnea larvae may have some potential in greenhouse crops, particularly against N. viridula infestations, whereas in open field crops their natural populations may also contribute to pest suppression within the broader predator guild.