The consumption of tropical fruits has been increasing throughout the world, especially in non-producing countries. Among the fruits in this category, bananas (Musa spp.) have consistently demonstrated a growing trend in both purchases and consumption throughout the world, and also in Italy. The following question therefore arises: is there a correspondence between the quality characteristics of bananas on the market and the consumers' preferences? The objectives of this study were twofold: 1) to qualitatively and sensorially characterize bananas at three different stages of commercial ripening (Banana quality assessment); and 2) to explore the consumption habits, perceptions, and preferences of Italian consumers regarding tropical fruits, focusing in particular on fruit quality, health, and sustainability (Consumers' perception of tropical fruits). The results indicated that ripe bananas were preferred by the sensory panel and maintained high-quality attributes even after five days of storage at room temperature. Moreover, the importance of the sustainability of tropical fruits and a preference for ripeness of bananas during the decision-making stage were found to be influenced by individual factors. Different levels of awareness and purchasing styles were identified. However, the connection between the findings of the sensory analysis and the declared preferences revealed several insights that can be used to improve managing the supply and assortment of bananas for distribution to satisfy the consumers’ preferences, improve communication strategies, encourage waste reduction, and enhance supply chain sustainability.