This paper focuses on the wind-induced vibration response of prestressed double-layered spherical latticed shell (PDSLS) structures by adopting time-domain analysis method. Welch spectrum analysis method is used to make precision evaluation of power spectrum of fluctuating wind speed time history simulated by weighted amplitude wavelet superposition (WAWS) method and linear filtering method of auto-regression (AR) model. Results show that the two methods produce little precision difference, but AR method is far more efficient than WAWS and is more suitable for wind speed simulation of PDSLSs. The effect of various parameters on the wind-induced vibration response of PDSLS structures are comprehensively investigated, including rise-span ratio, span, shell thickness, elastic constraint stiffness, prestress value, with or without cables and cable layout scheme. Results show that rise-span ratio and span are the major factors that affect wind-induced vibration response of PDSLSs. When cables are set, the wind vibration coefficient of nodal vertical displacement becomes smaller and more equally distributed, which demonstrates that PDSLSs are less sensitive to fluctuating wind effect than common latticed shell structures without cables. Finally, based on the envelopment concept and with the maximum dynamic and average wind-induced displacement responses as control indicators, the calculating method for global wind vibration coefficient (GWVC) of PDSLSs is proposed and the value with usual design parameters is given. Meanwhile, when the structure is made static analysis by means of the equivalent static wind load obtained from GWVC, the obtained internal member force response is relatively accordant with the actual response got from time-history analysis, and is a little safer.