Superparamagnetic Graphene Oxide Research Articles

Signature of the Kondo effect in superparamagnetic GO incorporated Cobalt substituted Ni/NiO nanoparticles

The study reports on the magnetization, magnetoresistance, and transport properties of superparamagnetic 10% Co-doped Ni/NiO (C10-NN), Graphene Oxide (GO) incorporated 10% Co-doped Ni/NiO (C10-NNG), and 15% Co-doped Ni/NiO (C15-NN) nanoparticles synthesized via a microwave-assisted sol-gel auto-combustion method. All samples show hysteresis in negative Magnetoresistance (M-R) at different temperatures. Resistivity ρ(T) versus temperature plots of samples C10-NN and C15-NN show metallic behavior with applied fields of 0, 1, 5, 8 T, and at 0 T, 1 T respectively. However, the plot of R-T of the C15-NN sample shows a significant difference at 0 T and 1 T. At 0 T for this sample, the metallic behavior is observed for temperature T > TM, with the resistivity falling abruptly at and above TM = 246 K. The resistivity decreases with increasing temperature, exhibiting metallic behavior again above TMM < 276 K. This jump at 276 K, indicating a metal-to metal transition. The Kondo effect is observed for the first time in C10-NNG sample. The upturn of resistivity ρ(T) towards low temperature in the C10-NNG sample is well described by the power series equation and Kondo term. This sample exhibits the upturn resistivity along with a metal–insulator transition above and below the Kondo temperature TK≈ 93.51(2) K at the 0 T, 1 T, 5 T, and 8 T fields.

Surface Engineering of Superparamagnetic Graphene Oxide Nanosheet as a Chemically Tunable Platform for Facial Biofuel Production by Lipase.

In this research, we utilized an efficient approach to synthesize superparamagnetic graphene oxide (SPGO) rapidly in a one-pot method using microwave irradiation of graphene oxide (GO), urea, and Fe(III) ion. Tannic acid (TA) was introduced to the surface of SPGO through a straightforward and eco-friendly process. Methods were devised to furnish GO nanosheets and modify their surfaces with TA in an environmentally friendly manner. Two series of nanosheets, namely, SPGO/TA-COOH and SPGO/TA-IM, were engineered on the surface and used for immobilizing lipase enzyme. Through various analytical tools, the unique biocatalysts SPGO/TA-COOH/L and SPGO/TA-IM/L were confirmed. These biocatalysts exhibited enhanced stability at high temperatures and pH levels compared with free lipase. They also demonstrated prolonged storage stability and reusability over four months and seven cycles, respectively. Furthermore, the catalytic activity of immobilized lipase showed minimal impairment based on kinetic behavior analysis. The kinetic constants of SPGO/TA-IM/L were determined as Vmax = 0.24 mM min-1, Km = 0.224 mM, and kcat = 0.8 s-1. Additionally, the efficiency of biocatalysts for biodiesel production from palmitic acid was studied, focusing on various reaction parameters, such as temperature, alcohol to palmitic acid molar ratio, water content, and lipase quantity. The esterification reaction of palmitic acid with methanol, ethanol, and isopropanol was tested in the presence of SPGO/TA-COOH/L and SPGO/TA-IM/L, and the corresponding esters were obtained with a yield of 30.6-91.6%.

The effect of 3D structure preparation method on lipase/3DMGO biocatalytic parameters and catalytic performance in transesterification of microalgae bio-oil

Nano-biocatalysts, based on Rhizopus oryzae lipase (ROL) immobilized on 3D superparamagnetic graphene oxide (3DMGO) were prepared in two methods and used for the conversion of Chlorella vulgaris oil to biodiesel. The 3D graphene oxide hydrogel was synthetized using two different cross-linking agents, p-phenylene diamine (PPD) and ethylene diamine tetra acetic acid (EDTA), and then magnetized by insitu co-participation of Fe3O4. A modified BG-11 medium was used for cultivation of this microalgae to increase the total biomass lipid (up to 35 wt%). The kinetics parameters, enzyme loading percentage, thermal stability, and storage stability of ROL, ROL/3DMGO-EDTA, and ROL/3DMGO-PPD were evaluated. The biodiesel production yields in the presence of ROL, ROL/3DMGO-PPD, and ROL/3DMGO-EDTA were 75.04%, 74.25%, and 72.12%, respectively. The reusability tests confirmed that the ROL/3DMGO-EDTA had better performance. Therefore, 3D graphene oxide nanostructures can be introduced as a suitable option for enzyme immobilization in the biodiesel production from microalgae oil.

Synthesis and characterization of superparamagnetic graphene oxide assembled halloysite composites for extraction of rutin

In this work, a novel composite magnetic graphene oxide@magnetic halloysite nanotubes (MGO@magnetic Hal nanotube) was constructed to serve as an efficient solid phase adsorption tool for the separation of rutin by covalent binding of 3-aminopropyltrimethoxysilane (APTES)-functionalized magnetic graphene oxide (MGO) and magnetic halloysite (Hal) nanotubes. In particular, the amino group of APTES reacted with the epoxy bond on the basal planes of MGO instead of the edges carboxyl group, so that magnetic Hal nanotube could be inserted between the layers of MGO to prevent aggregation more effectively. In order to prove that the composite was synthesized in the ideal way, characterization tests such as FT-IR, XPS, SEM and HRTEM were made in detail. More importantly, the MGO@magnetic Hal nanotube possessed stronger super magnetism (40.82 emu g−1) than MGO and magnetic Hal nanotube by VSM characterization. In addition, further experiments show the adsorption process could be described by pseudo-second-order model and Freundlich isotherm model. Besides, the adsorption capacity of rutin by the composite was also greater than that of rutin by the single material. Some key parameters in the process of adsorption and desorption were also optimized. Finally, MGO@magnetic Hal nanotube was successfully used for the extraction of rutin from health products because of its excellent magnetism and adsorption.

Superparamagnetic nanoadsorbents for the removal of trace As(III) in drinking water

A series of novel zeolitic imidazolate framework (ZIF) decorated superparamagnetic graphene oxide hybrid nanoadsorbents were synthesized, characterized, and tested for their As(III) adsorbed amount in simulated drinking water. The three composite nanomaterials are based each on three isostructural and water stable ZIFs, (C-1 based on ZIF-8, C-2 based on ZIF-67, and C-3 based on ZIF-Zn/Co). The composite nanomaterials and there parent materials were characterized through pXRD, TEM, FTIR, BET and magnetometry methods (SQUID), and were tested as adsorbents in a representative drinking water matrix containing arsenite (As(III)) at an initial trace concentration (realistic in some natural drinking water sources) of 35 µg/L. The nanoadsorbents were magnetically captured and removed after adsorption in batch conditions. Out of the three composites, C-2 shows the highest As(III) adsorbed amount at an initial concentration of 35 µg/L (q0) of 202 µg/g, followed by C-3 with 102 µg/g and C-1 with 82 µg/g.

Functionalized graphene oxide/Fe3O4 nanocomposite: A biocompatible and robust nanocarrier for targeted delivery and release of anticancer agents

The development of efficient drug nanocarriers has remained an important challenge in advanced drug delivery in human body. Combination of graphene-based nanomaterials and cyanuric chloride (CC), as a linker, may improve the success of drug delivery. Herein, a simple approach was used for the synthesis of superparamagnetic graphene oxide (SPMGO) nanocomposite through a chemical precipitation method. The nanocomposite was readily functionalized with cyanuric chloride as a linker for loading the drug. The FTIR spectroscopy confirmed the efficient synthesis of nanocarriers. So did the transmission electron microscopy, atomic force microscopy, and thermo-gravimetric analysis, X-ray diffraction and X-ray photoelectron spectroscopy. Subsequently, the synthesized nanocarriers were studied in terms of their potential for biomedical applications. Immobilization of methotrexate (MTX), as a drug for treatment of cancer was taken into action on the SPMGO and SPMGO/CC. The in vitro assays indicated that the drug nanocarrier systems, SPMGO/MTX and SPMGO/CC/MTX, are hemo-compatible and increase the efficiency of MTX against Caov-4, HeLa and MCF-7 cell lines. The MTX nanocarriers represented a considerably high drug loading and controlled drug release. The overall results indicated the great potential of SPMGO/CC/MTX nanocarrier for targeted drug delivery, particularly in MTX chemotherapy.

Poly(Vinyl Alcohol) Enhances Acetylation of Ascorbic Acid in Superparamagnetic Graphene Oxide Nanoparticles Ultrasonically Complexed with Acetylsalicylic Acid

A single-step ultrasonic method (20 kHz) is demonstrated for the formation of acetylsalicylic acid-Fe3O4-graphene oxide nanocomposites (∼80 nm) in aqueous solution. The electronic molecular structu...

In situ assembly of magnetic nanocrystals/graphene oxide nanosheets on tumor cells enables efficient cancer therapy

Owing to the stimulus-responsive and dynamic properties, magnetism-driven assembly of building blocks to form ordered structures is always a marvelous topic. While abundant magnetic assemblies have been developed in ideal physical and chemical conditions, it remains a challenge to realize magnetic assembly in complicated biological systems. Herein, we report a kind of biomacromolecule-modified magnetic nanosheets, which are mainly composed of superparamagnetic graphene oxide (γ-Fe2O3@GO), the tumor-targeting protein transferrin (TF), and the mitochondrion-targeting peptide (MitP). Such large-size nanosheets (0.5–1 μm), noted as L-Fe2O3@GO-MitP-TF, can successfully in situ assemble on the surface of tumor cells in a size-dependent and tumor cell-specific way, leading to severe inhibition of nutrient uptake for the tumor cells. More significantly, the nanostructures could efficiently confine the tumor cells, preventing both invasion and metastasis of tumor cells both in vitro and in vivo. Moreover, the 2D assemblies could remarkably disrupt the mitochondria and induce apoptosis, remarkably eradicating tumors under near-infrared (NIR) irradiation. This study sheds light on the development of new nano-systems for efficient cancer therapy and other biomedical applications.

Green and Solvent-Free Supercritical CO2-Assisted Production of Superparamagnetic Graphene Oxide Aerogels: Application as a Superior Contrast Agent in MRI

This work was financed by the Spanish National Plan of Research with projects CTQ2017-83632 and MAT2017-86616-R. C.D., G.T., A.B, J.F., and A.M.L.P. acknowledge the financial support from the Spanish MEC, through the Severo Ochoa Program for Centres of Excellence in R&D (SEV-2015-0496). This work (A.B.) has been done in the framework of the doctoral program “Chemistry” of the Universitat Autonoma de Barcelona. A.M.L.P. acknowledges the RyC-2012-11588 contract. A.B. acknowledges the FPI grant.

Magnetic Properties of Poly(trimethylene terephthalate‐block‐Poly(tetramethylene oxide) Copolymer Nanocomposites Reinforced by Graphene Oxide–Fe3O4 Hybrid Nanoparticles

Thermoplastic elastomeric nanocomposites based on poly(trimethylene terephthalate‐block‐poly(tetramethylene oxide) copolymer (PTT‐PTMO) and graphene oxide (GO)–Fe3O4 nanoparticles hybrid are prepared by in situ polymerization. Superparamagnetic GO–Fe3O4 hybrid nanoparticles before introducing to elastomeric matrix are characterized by X‐ray photoelectron spectroscopy (XPS), X‐ray diffraction (XRD), thermogravimetric analysis (TGA), and scanning electron microscopy (SEM). The effect of loading (0.3 and 0.5 wt%) of GO–Fe3O4 nanoparticle hybrid on the phase structure, tensile, and magnetic properties of synthesized nanocomposites is investigated. The phase structure of nanocomposites is evaluated by differential scanning calorimetry (DSC) and dynamic mechanical thermal analysis (DMTA). Dispersion of GO–Fe3O4 nanoparticles in elastomeric matrix is evaluated by transmission electron microscopy (TEM). Magnetic properties of GO–Fe3O4 nanoparticle hybrid and nanocomposites with their content are characterized using two different techniques: direct current superconducting quantum interference device (dc SQUID) magnetization measurements as a function of temperature (from 2 to 300 K) and external magnetic field and ferromagnetic resonance (FMR) at microwave frequency.

Preparation of polyglycerol mediated superparamagnetic graphene oxide nanocomposite and evaluation of its adsorption properties on tetracycline

In this paper, we synthesized a polyglycerol(PG)-mediated superparamagnetic graphene oxide nanocomposite called MGON, consisting of PG-modified superparamagnetic iron oxide nanoparticles (SPION) covalently bonded to PG-functionalized graphene oxide (GO). MGON exhibits better dispersibility and colloidal stability in aqueous solution than the magnetic graphene oxide reported in the literature. The physicochemical properties of MGON were analyzed by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS), transmission electron microscopy (TEM), vibrating sample magnetometer (VSM), and UV-vis spectroscopy. Applied to the adsorption of tetracycline (TC) in aqueous solution as an adsorbent, the MGON showed excellent adsorption performance with the maximum adsorption capacity of 684.93mg/g at 298K. Adsorption kinetics and isotherm results indicate that the adsorption process conforms to the pseudo-second-order kinetics and Langmuir isotherm models. Adsorption thermodynamics has confirmed that the adsorption process of TC on MGON is spontaneous and endothermic. With the increase of temperature, the adsorption capacity of MGON increases continuously, and the adsorption capacity of MGON is the largest when the pH value is 7. Furthermore, the π-π and cation-π interaction, amidation reaction, and hydrogen bonding can be used to explain the adsorption mechanism of TC on MGON. Desorption and regeneration experiments showed that MGON still had 67.65% regenerative performance after five cycles. Hence, MGON is a promising adsorbent in the removal of tetracycline from wastewater.

Superparamagnetic enzyme-graphene oxide magnetic nanocomposite as an environmentally friendly biocatalyst: Synthesis and biodegradation of dye using response surface methodology

The unique properties of graphene oxide (GO) nanosheets were integrated with the superparamagnetic characteristics of the CuFe2O4 nanoparticles to synthesize the magnetic graphene oxide (MGO), which was chemically modified with 3-amino propyl trimethoxy silane (APTMS) to functionalize the amine group on MGO (MGO-NH2). Afterward, MGO-NH2 was activated with glutaraldehyde (GLU) as a crosslinking agent to synthesize the functionalized MGO (fMGO) and its capability toward covalent Laccase immobilization was investigated. The comprehensive structural analysis using various characterization techniques, including Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), vibrating sample magnetometer (VSM), transmission electron microscopy (TEM), and scanning electron microscopy (SEM) clearly confirmed the covalent attachment of laccase onto MGO. The response surface methodology (RSM), based on Central Composite Design (CCD), was applied to optimize the immobilized Laccase (nanobiocatalyst)-mediated biodegradation of Direct Red 23 (DR23), as an azo dye, by considering independent variables such as nanobiocatalyst dosage, dye concentration, and pH. The optimal conditions to obtain the maximum decolorization yield (95.33%) were nanobiocatalyst dosage = 290.23 mg/L, dye concentration = 19.60 mg/L, and pH = 4.23. The obtained correlation coefficient and the adjusted one of 0.9651 and 0.9336, respectively, imply the nice model fitness. Therefore, structural characterization along with the experimental decolorization results introduced the synthesized superparamagnetic GO as an environmentally friendly nanobiocatalyst for effective decolorization purposes.

Covalent immobilization of xylanase from Thermomyces lanuginosus on aminated superparamagnetic graphene oxide nanocomposite

Today, immobilization of enzymes has been extensively considered in a wide variety of applications in industries. Xylanase is a hemicellulotic enzyme with effective usages that has attracted the attention of researchers. In this work, xylanase from Thermomyces lanuginosus was covalently immobilized on modified graphene oxide nanosheets (NGO) via cyanuric chloride linker. The magnetic nanoparticles were perched on NGO to facilitate separation of nanocarrier from the reaction media. To decrease the steric hindrance and allow the high-molecular weight xylan as a substrate to approach the active site of the immobilized xylanase, polyethylene glycol bis-amine was used as a spacer. Various techniques such as TEM, SEM, FTIR, VSM and TGA were applied to characterize the structure of synthetic nanocomposite. The amount of immobilized xylanase was quantified by Bradford assay, and the immobilization efficiency was determined about 67%. The results showed that thermal stability, pH stability, reusability and storage stability were improved for immobilized enzyme and the kinetic parameters, $${K_m}$$ and $${v_{\hbox{max} }}$$ values, were increased, suggesting the great potential of the functionalized NGO as a novel nanocarrier in bio-industry applications.

In situ co-precipitation preparation of a superparamagnetic graphene oxide/Fe3O4 nanocomposite as an adsorbent for wastewater purification: synthesis, characterization, kinetics, and isotherm studies.

A superparamagnetic graphene oxide (GO)/Fe3O4 nanocomposite (MGO) was prepared by a facile in situ co-precipitation strategy, resulting in a prospective material for the application of graphene oxide in wastewater treatment. MGO was characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), x-ray diffraction (XRD), and Fourier transform infrared spectroscopy (FTIR). The prepared adsorbent showed a high adsorption efficiency relevant to the purification of dye-contaminated wastewater and could be readily magnetically separated. The maximum adsorption capacity was ca. 546.45mgg-1 for the common cationic dye methylene blue (MB) and ca. 628.93mgg-1 for the anionic dye Congo red (CR). The adsorption processes fit the pseudo-second-order kinetic model well, which revealed that these processes may involve the chemical interaction between adsorbate and adsorbent. The thermodynamic parameters indicated that the adsorption reaction was an endothermic and spontaneous process. Furthermore, the prepared magnetic adsorbent had a wide effective pH range from 5 to 11 and showed good stability after five reuse cycles. The synthetic MGO showed great potential as a promising adsorbent for organic contaminant removal in wastewater treatment.

Multifaceted Implantable Anticancer Device for Potential Postsurgical Breast Cancer Treatment: A Single Platform for Synergistic Inhibition of Local Regional Breast Cancer Recurrence, Surveillance, and Healthy Breast Reconstruction

AbstractAn implantable anticancer device (IAD) amenable to eradicate local regional recurrence (LRR) of breast cancer as well as to enhance the breast reconstruction during/after therapy is proposed for the first time. The IAD is fabricated by incorporating the superparamagnetic graphene oxide doxorubicin nanocomposite of size ≈200 nm in a nanofiber matrix to enable the sustained and tumor specific anticancer drug release over 60 d in vitro with a minimum initial burst release and the repeated hyperthermic capability in an alternating magnetic field to ensure the synergistic inhibition of LRR. The IAD also enriches the reconstruction of poor breast cosmesis that resulted from the surgical treatment by supporting the lipofilling of the surgical residual cavity to induce the adipogenic process. Moreover, the noninvasive monitoring capability of IAD through magnetic resonance imaging augments to the effective patient care. Thus, this work reports a new and reliable concept by introducing the multifunctional IAD possessing enhanced specificity with a real‐time monitoring capability and long‐term anticancer efficacy as a potential platform for synergistic inhibition of LRR and a great promise for the in situ breast reconstruction ability for better breast cosmesis.

Xylanase immobilization on modified superparamagnetic graphene oxide nanocomposite: Effect of PEGylation on activity and stability

In order to utilize the advantages of immobilization such as improvement of stability, increasing the catalytic activity, ability to recovery and reuse of enzyme from reaction medium, xylanase enzyme was immobilized on superparamagnetic garphene oxide nanosheets (GOMNP). Xylanase, as a hydrolytic enzyme of xylan has widely used in industry. Since the xylan is bulk, for enhance accessibility of active sites of the immobilized xylanase, polyethylene glycol bis amine (PEGA) was used as a spacer for functionalization of GOMNP. The modified GOMNP and immobilized xylanase on PEGA-GOMNP (PEGA-GOMNP/Xy) were characterized through different analysis tools. The results showed that xylanase was attached to the functionalized nanocomposite with a yield of 273mg enzyme per gram PEGA-GOMNP. Thermal stability, pH stability, reusability and storage stability were determined for immobilized enzyme. The free and immobilized xylanase displayed an optimal enzymatic activity at 60°C and pH 6.5 and 7.5, respectively. The immobilized enzyme retained about 40% of the initial activity after 8 cycles with xylan substrate at 60°C. Also immobilized and free enzymes retained 35% and 20% of the initial catalytic activity after 90days storage at 4°C, respectively. Consequently, PEGA- modified GOMNP can be introduced as a biodegradable and suitable support for bioengineering.

Superparamagnetic graphene oxide–magnetite nanoparticle composites for uptake of actinide ions from mildly acidic feeds

Super paramagnetic graphene oxide (GO) − Fe3O4 nanoparticle composites were prepared and characterized by conventional techniques such as XRD, SEM, EDX, FT-IR, Raman, XPS, DLS and zeta potential, etc. TEM studies have confirmed nanoparticle nature of the composites. The GO-magnetic nanoparticle composites can be dispersed in mildly acidic aqueous solutions and get concentrated in a small volume under application of an external magnetic field. The composites were evaluated for the uptake of actinide ions such as Am3+, UO22+, Th4+ and Pu4+ from mildly acidic aqueous solutions. Am3+ sorption sharply increased with pH as the Kd values increased from about 10 at pH 1 to 105 at pH 3 beyond which a plateau in the Kd values was seen. Eu3+ displayed nearly comparable uptake behaviour to that of Am3+ while the uptake of other metal ions followed the trend: Pu(IV)>Th(IV)>>UO22+. The adsorption behaviour of Am3+ onto the graphene oxide − Fe3O4 nanoparticle composites fitted very well to the Langmuir as well as Temkin isotherm models. The desorption rate (using 1M HNO3) was fast and reusability study results were highly encouraging. The very high uptake values suggest possible application of the magnetic nanoparticles in radioactive waste remediation in natural ground water.

Binary adsorption of 17β-estradiol and bisphenol A on superparamagnetic amino-functionalized graphene oxide nanocomposites

Two types of graphene oxide (GO), namely, superparamagnetic GO (SM-GO) and chemically modified superparamagnetic amino-functionalized GO (SM-NGO), were prepared in this study. The adsorption of 17β-estradiol (E2) and bisphenol A (BPA) by the two types of GO composites was investigated. SM-NGO showed better adsorptive performance than SM-GO in adsorbing E2 and BPA. Moreover, E2 was suitably removed by both adsorbents, which indicated the stronger interaction of E2 with the composites than BPA. The performance of the sorbents was examined through pH, kinetic, and isotherm experiments. The adsorption of E2 and BPA was favorable under neutral condition. The rate-limiting step in the adsorption process was chemisorption for both adsorbents, which fit well with the pseudo-second-order kinetic model. The adsorption isotherms were described by the Langmuir model. Moreover, the theoretical maximum adsorption capacity (Qmax) of E2 by SM-NGO and SM-GO reached 106.38 and 52.91 mg g−1, respectively, and the corresponding Qmax values for BPA were 41.32 and 18.11 mg g−1. Both nanocomposites can be easily separated by a commercial magnet within a few seconds. Therefore, SM-NGO is a promising magnetic adsorbent of heterogeneous organic chemicals.

Functionalized superparamagnetic graphene oxide nanosheet in enzyme engineering: A highly dispersive, stable and robust biocatalyst

Immobilized enzymes are used as biocatalysts for analytical purposes in diagnostics and for preparative purposes in large scale industrial processes. Immobilization can increase the half-life, improve the stability, and increase the catalytic activity of an enzyme. It facilitates the separation and recovery of the catalyst from the reaction products, and allows for multiple use of the biocatalysts. Graphene oxide nanosheets were decorated with superparamagnetic iron oxide nanoparticles (SPGO) and functionalized with cyanuric chloride (SPGO-CC) to serve as solid support for the covalent immobilization of enzymes. Xylanase was attached to the functionalized nanocomposite with a yield of 215mg protein pergram SPGO-CC. The kinetic constants of the immobilized (of the free soluble) xylanase are Km=4.9 (4.1)mg/mL, vmax=1.6 (1.7)μmolmin−1mL−1, and kcat=82 (91)min−1, indicating a minimal impairment of catalytic activity by covalent coupling. The immobilized xylanase has shallow pH and temperature optima of 6.5 and 60°C. It retains 70% of the original activity after 10 cycles of 15min incubation with substrate (polymeric xylan) at 60°C. It retains 80% of the original catalytic activity after 3.5months storage at 4°C, whereas the free, soluble enzyme retains only 50%.

Functionalized graphene oxide-based thermosensitive hydrogel for magnetic hyperthermia therapy on tumors

A novel locally injectable, biodegradable, and thermo-sensitive hydrogel made from chitosan and β-glycerophosphate salt was prepared. It incorporated polyethylenimine (PEI)-modified super-paramagnetic graphene oxide (GO/IONP/PEI) as a form of minimally invasive treatment of cancer lesions by magnetically induced local hyperthermia. Doxorubicin (DOX) was mixed into the hydrogel which was pre-loaded on GO/IONP/PEI to create a drug delivery system DOX-GO/IONP/PEI-gel. In addition to the evaluation of in vitro and in vivo antitumor activities, the physicochemical properties, magnetic properties and DOX release profile of the DOX-GO/IONP/PEI-gel were determined. The aqueous solution of the hydrogel showed a sol-gel transition behavior depending on temperature changes. Magnetization loops indicated the super-paramagnetic properties of GO/IONP/PEI. Compared with free DOX, DOX-GO/IONP/PEI could efficiently pass through cell membranes, leading to more apoptosis and demonstrating higher antitumor efficacy on MCF-7 cells in vitro. Furthermore, DOX-GO/IONP/PEI-gel intratumorally injected (i.t.) showed high antitumor efficacy on tumor-bearing mice in vivo, with no obvious toxicity. The antitumor efficacy was higher when combined with an alternating magnetic field (AMF), showing that DOX-GO/IONP/PEI-gel under AMF has great potential for cancer magnetic hyperthermia therapy.