Dismutation Of Superoxide Research Articles

Red blood cells from patients with ST-elevation myocardial infarction and elevated C-reactive protein levels induce endothelial dysfunction.

Endothelial dysfunction is an early consequence of vascular inflammation and a driver of coronary atherosclerotic disease leading to myocardial infarction. The red blood cells (RBCs) mediate endothelial dysfunction in patients at cardiovascular risk, but their role in patients with acute myocardial infarction is unknown. This study aimed to investigate if RBCs from patients with ST-elevation myocardial infarction (STEMI) induced endothelial dysfunction and the role of systemic inflammation in this effect. RBCs from patients with STEMI and aged-matched healthy controls were co-incubated with rat aortic segments for 18h followed by evaluation of endothelium-dependent (EDR) and -independent relaxation (EIDR). RBCs and aortic segments were also analyzed for arginase and oxidative stress. The patients were divided into groups depending on C-reactive protein (CRP) levels at admission. RBCs from patients with STEMI and CRP levels >2 mg/L induced impairment of EDR, but not EIDR, compared to RBCs from STEMI and CRP <2 mg/L and healthy controls. Aortic expression of arginase 1 was increased following incubation with RBCs from patients with STEMI and CRP >2, and arginase inhibition prevented the RBC-induced endothelial dysfunction. RBCs from patients with STEMI and CRP >2 had increased reactive oxygen species compared to RBCs from patients with CRP <2 and healthy controls. Vascular inhibition of NADPH oxidases and increased dismutation of superoxide improved EDR. RBCs from patients with STEMI and low-grade inflammation induce endothelial dysfunction through a mechanism involving arginase 1 as well and increased RBC and vascular superoxide by NADPH oxidases.

An Enzyme-Encapsulated MOF@MOF Nanocomposite for Detecting H2O2 Derived From Superoxide Anion Released by Mitochondria of HeLa Cells.

In this work, a horseradish peroxidase (HRP)-encapsulated metal organic framework (MOF)@MOF nanocomposite is developed for detecting H2O2 converted by dismutation of superoxide anions released from live HeLa mitochondria. Initially, an HRP-polyacrylic acid cluster is incorporated on a mesoporous, peroxidase-like Cu/Co-1,4-benzenedicarboxylate (BDC) MOF platform to avoid structural change and deactivation of HRP through its interactions with MOF metal ions. Additionally, a Cu/Co-BDC(HRP)@1,3,5-benzenetricarboxyate (BTC) core-shell MOF/MOF structure, also with peroxide-like properties, serves as a protective matrix for HRP. Then, ultrathin porous carbon shells (UPCS) are adopted to improve the electrical conductivity of the MOF@MOF. The Cu/Co-BDC(HRP)@BTC|UPCS sensing platform exhibits two linear ranges of 0.05-1 µM and 1-1000 µM with a sensitivity of 172mA mM-1 cm-2 and 1.63mA mM-1 cm-2, respectively. A limit of detection of 0.057 µM, good selectivity and stability over 35 days for H2O2 detection are also achieved. After treating the mitochondrial complex with specific inhibitors, amperometric results at the sensing platform confirmed complex I and III within mitochondria as the main electron leakage sites in the electron transfer chains. Therefore, this sensing platform provides a tool that may aid in predicting and even developing treatments for some oxidative stress diseases caused by mitochondrial abnormalities.

Publisher Correction: Heat Stress Modulates Superoxide and Hydrogen Peroxide Dismutation and Starch Synthesis during Tuber Development in Potato

Publisher Correction: Heat Stress Modulates Superoxide and Hydrogen Peroxide Dismutation and Starch Synthesis during Tuber Development in Potato

Heat Stress Modulates Superoxide and Hydrogen Peroxide Dismutation and Starch Synthesis during Tuber Development in Potato

Heat Stress Modulates Superoxide and Hydrogen Peroxide Dismutation and Starch Synthesis during Tuber Development in Potato

Computational Analysis of the Superoxide Dismutase Mimicry Exhibited by a Zinc(II) Complex with a Redox-Active Organic Ligand.

Previously, we found that a Zn(II) complex with the redox-active ligand N-(2,5-dihydroxybenzyl)-N,N',N'-tris(2-pyridinylmethyl)-1,2-ethanediamine (H2qp1) was able to act as a functional mimic of superoxide dismutase, despite its lack of a redox-active transition metal. As the complex catalyzes the dismutation of superoxide to form O2 and H2O2, the quinol in the ligand is believed to cycle between three oxidation states: quinol, quinoxyl radical, and para-quinone. Although the metal is not the redox partner, it nonetheless is essential to the reactivity since the free ligand by itself is inactive as a catalyst. In the present work, we primarily use calculations to probe the mechanism. The calculations support the inner-sphere decomposition of superoxide, suggest that the quinol/quinoxyl radical couple accounts for most of the catalysis, and elucidate the many roles that proton transfer between the zinc complexes and buffer has in the reactivity. Acid/base reactions involving the nonmetal-coordinating hydroxyl group on the quinol are predicted to be key to lowering the energy of the intermediates. We prepared a Zn(II) complex with N-(2-hydroxybenzyl)-N,N',N'-tris(2-pyridinylmethyl)-1,2-ethanediamine (Hpp1) that lacks this functional group and found that it could not catalyze the dismutation of superoxide; this confirms the importance of the second, distal hydroxyl group of the quinol.

Non-Thyroidal Illness in Chronic Renal Failure: Triiodothyronine Levels and Modulation of Extra-Cellular Superoxide Dismutase (ec-SOD).

Oxidative stress (OS) is implicated in several chronic diseases. Extra-cellular superoxide dismutase (ec-SOD) catalyses the dismutation of superoxide anions with a protective role in endothelial cells. In chronic kidney disease (CKD), OS and thyroid dysfunction (low fT3 syndrome) are frequently present, but their relationship has not yet been investigated. This cohort study evaluated ec-SOD activity in CKD patients during haemodialysis, divided into "acute haemodialytic patients" (AH, 1-3 months of treatment) and "chronic haemodialytic patients" (CH, treated for a longer period). We also evaluated plasmatic total antioxidant capacity (TAC) and its relationships with thyroid hormones. Two basal samples ("basal 1", obtained 3 days after the last dialysis; and "basal 2", obtained 2 days after the last dialysis) were collected. On the same day of basal 2, a sample was collected 5 and 10 min after the standard heparin dose and at the end of the procedure. The ec-SOD values were significantly higher in CH vs. AH in all determinations. Moreover, the same patients had lower TAC values. When the CH patients were divided into two subgroups according to fT3 levels (normal or low), we found significantly lower ec-SOD values in the group with low fT3 in the basal, 5, and 10 min samples. A significant correlation was also observed between fT3 and ec-SOD in the basal 1 samples. These data, confirming OS and low fT3 syndrome in patients with CKD, suggest that low fT3 concentrations can influence ec-SOD activity and could therefore potentially contribute to endothelial oxidative damage in these patients.

Identification of Superoxide Dismutase (SOD) Isozymes in Plant Tissues.

Superoxide and hydrogen peroxide are reactive oxygen species (ROS) involved in the oxidation of multiple biological molecules and the signaling processes during plant growth and stress response. Thus, control of ROS is fundamental for cell survival and development, with superoxide dismutase (EC 1.15.1.1, SOD) being one of the main enzymes involved. Different isoforms of SOD catalyze the dismutation of superoxide (O2.-) to hydrogen peroxide (H2O2) and oxygen (O2), such as Mn-SODs, Cu,Zn-SODs, and Fe-SODs. Using non-denaturing polyacrylamide gel electrophoresis (PAGE) combined with a specific staining method for SOD activity, the protocol describes the identification of different SOD isozymes, based on their differential inhibition by KCN and H2O2, in different organs and plant species such as pea (Pisum sativum L.) leaves and pepper (Capsicum annuum L.) fruits.

Superoxide dismutase gene family in cassava revealed their involvement in environmental stress via genome-wide analysis

Superoxide dismutase (SOD) is a crucial metal-containing enzyme that plays a vital role in catalyzing the dismutation of superoxide anions, converting them into molecular oxygen and hydrogen peroxide, essential for enhancing plant stress tolerance. We identified 8 SOD genes (4 CSODs, 2 FSODs, and 2 MSODs) in cassava. Bioinformatics analyses provided insights into chromosomal location, phylogenetic relationships, gene structure, conserved motifs, and gene ontology annotations. MeSOD genes were classified into two groups through phylogenetic analysis, revealing evolutionary connections. Promoters of these genes harbored stress-related cis-elements. Duplication analysis indicated the functional significance of MeCSOD2/MeCSOD4 and MeMSOD1/MeMSOD2. Through qRT-PCR, MeCSOD2 responded to salt stress, MeMSOD2 to drought, and cassava bacterial blight. Silencing MeMSOD2 increased XpmCHN11 virulence, indicating MeMSOD2 is essential for cassava's defense against XpmCHN11 infection. These findings enhance our understanding of the SOD gene family's role in cassava and contribute to strategies for stress tolerance improvement.

Initial characterization of an iron superoxide dismutase from Thermobifida fusca

Superoxide dismutases (SODs) are enzymes that catalyze the dismutation of the superoxide radical anion into O2 and H2O2 in a two-step reaction. They are ubiquitous to all forms of life and four different types of metal centers are detected, dividing this class of enzymes into Cu-/Zn-, Ni-, Mn-, and Fe-SODs. In this study, a superoxide dismutase from the thermophilic bacteria Thermobifida fusca (TfSOD) was cloned and expressed before the recombinant enzyme was characterized. The enzyme was found to be active for superoxide dismutation measured by inhibition of cytochrome c oxidation and the inhibition of the autoxidation of pyrogallol. Its pH-optimum was determined to be 7.5, while it has a broad temperature optimum ranging from 20 to 90 °C. Combined with the Tm that was found to be 78.5 ± 0.5 °C at pH 8.0, TfSOD can be defined as a thermostable enzyme. Moreover, the crystal structure of TfSOD was determined and refined to 1.25 Å resolution. With electron paramagnetic resonance spectroscopy, it was confirmed that iron is the metal co-factor of TfSOD. The cell potential (Em) for the TfSOD-Fe3+/TfSOD-Fe2+ redox couple was determined to be 287 mV.Graphical abstract

Mechanistic Insights into Superoxide Dismutation Driven by Dinuclear Manganese Complexes: The Role of the Mn2-Core

The dinuclear Mn2(II,II)L2-core (HL = 2-{[[di(2-pyridyl)methyl](methyl)amino]-methyl}phenol) has been recently reported to be the most active dual superoxide dismutase (SOD) and catalase (CAT) functional analogue, enabling cascade detoxification of the superoxide radical anion. Here, we investigated the mechanism of catalytic O2•– decomposition by two stereoisomers with the Mn2(II,II)L2-core, Mn2L2Ac and Mn2L2, in order to (i) precisely determine the catalytic SOD activity of the complexes, (ii) characterize the key intermediates involved in the dismutation process, and (iii) discriminate between single- and di-Mn center catalysis in relation to the configuration of the Mn2-core. The conclusions drawn from low-temperature mass spectrometry, stopped-flow kinetics, cyclic voltammetry, water exchange 17O nuclear magnetic resonance (NMR), and electron paramagnetic resonance (EPR) analyses were supported by the structural characterization and quantum chemical analysis of the proposed reaction intermediates. This study allows us to determine kcat for Mn2L2Ac and Mn2L2 (4.6 × 107 and 2.2 × 107 M–1 s–1, respectively, in 3-(N-morpholino)propanesulfonic acid (MOPS) at pH = 7.4) and detect the key intermediates involved in the catalytic cycle driven by these Mn2-SOD mimics, highlighting the formation of a side-on η2–Mn2(III,II)–peroxo, as an initial intermediate. The effects of the Mn2(II,II)-core configuration on the SOD activity were discussed.

Microglial inflammatory reactions regulated by oxidative stress.

Microglia are immune cells in the brain that can respond to endogenous and exogenous substrates to elicit inflammatory reactions. The transcription factor nuclear factor kappa-light-chain-enhancer of activated B induces proinflammatory gene expression in response to foreign matter via pattern recognition receptors; thus, nuclear factor kappa-light-chain-enhancer of activated B is a master regulator of inflammation. During the inflammatory process, very large amounts of reactive oxygen species are generated and promote the onset and progression of inflammation. Interestingly, nuclear factor kappa-light-chain-enhancer of activated B drives the transcription of superoxide dismutase 2 in many types of cells, including microglia. Superoxide dismutase 2 is an antioxidative enzyme that catalyzes the dismutation of superoxide anions into molecular oxygen and hydrogen peroxide. Of note, nuclear factor kappa-light-chain-enhancer of activated B can initiate inflammation to elicit proinflammatory gene expression, while its transcription product superoxide dismutase 2 can suppress inflammation. In this review, we use recent knowledge to describe the interaction between oxidative stress and nuclear factor kappa-light-chain-enhancer of activated B and discuss the complicated role of microglial superoxide dismutase 2 in inflammation.

Application of a synthetic phenol-type antioxidant for cryopreservation of Russian sturgeon (Acipenser gueldenstaedtii) sperm

Antioxidative and cryoprotective effects of a novel synthetic antioxidant–1,1'-(piperazine-1,4-diyl)bis(2-(2-hydroxyphenylthio)ethanone (AO) is presented in comparison with commonly known antioxidant butylated hydroxytoluene (BHT). Superoxide anion radical scavenging activity of new AO and the increase in superoxide dismutation activity of the Russian sturgeon’s (Acipenser gueldenstaedtii) sperm were established in the presence of this compound. It was shown that the new phenol agent reduces level of carbonyl oxidation by-products, which can react with thiobarbituric acid (TBARS), in Russian sturgeon’s native and frozen/thawed sperm, which indicates the manifestation of antioxidant properties by this compound including under the action of damaging factors during cryopreservation. A beneficial effect of this phenol derivative on the activity indicators (the percentage of motile sperm cells and total period of sperm movement) of the Russian sturgeon’s frozen/thawed sperm was shown, which indicates the cryoprotective effect of a novel synthetic antioxidant. The efficiency of the AO exceeds the effect of BHT under cryopreservation conditions of Russian sturgeon sperm in the presence of the modified Stein’s medium, but further studies are needed on the effect of a new phenol agent on the fertilizing capacity of Russian sturgeon’s sperm.

Synthesis, characterization and superoxide dismutase activity of a biomimetic Mn(III) complex covalently anchored to mesoporous silica

A mononuclear Mn(III) complex of a clickable ligand, [Mn(hbpapn)(H2O)2]ClO4·4.5H2O, where H2hbpapn = 1,3-bis[(2-hydroxybenzyl)(propargyl)amino]propane, has been prepared and fully characterized. The complex catalyzes the dismutation of superoxide employing a Mn(III)/Mn(IV) redox cycle, with catalytic rate constant of 3.9 × 106 M−1 s−1 determined through the nitro blue tetrazolium photoreduction inhibition assay, in aqueous medium of pH 7.8. The alkyne function of the ligand was used for the covalent attachment of the catalyst to azide modified mesoporous silicas with different texture and morphology, through click chemistry. In these materials the catalyst is essentially linked to the inner pore walls, isolated and protected from the external medium. The hybrid materials can be recycled, and retain or improve the superoxide dismutase activity of the free catalyst with the pore size of the solid matrix playing a role on the activity of the catalyst.

Two Distinct Superoxidase Dismutases (SOD) Secreted by the Helminth Parasite Fasciola hepatica Play Roles in Defence against Metabolic and Host Immune Cell-Derived Reactive Oxygen Species (ROS) during Growth and Development.

The antioxidant superoxide dismutase (SOD) catalyses the dismutation of superoxide, a dangerous oxygen free radical, into hydrogen peroxide and molecular oxygen. Superoxide generation during the oxidative burst of the innate immune system is considered a key component of the host defence against invading pathogens. We demonstrate the presence and differential expression of two SODs in Fasciola hepatica, a leaderless cytosolic (FhSOD1) and an extracellular (FhSOD3) form containing a secretory signal peptide, suggesting that the parasites exploit these enzymes in distinct ways to counteract reactive oxygen species (ROS) produced by cellular metabolism and immune defences. Both enzymes are highly expressed by the infective newly excysted juvenile (NEJ) stages and are found in abundance in their excretory–secretory products (ES), but only FhSOD1 is present in adult ES, suggesting that the antioxidants have different functions and pathways of secretion, and are under separate temporal expression control during the migration, growth, and development of the parasite. Functionally, the recombinant FhSOD1 and FhSOD3 exhibit similar activity against superoxide to their mammalian counterparts. Confocal immuno-localisation studies demonstrated the presence of FhSOD1 and FhSOD3 on the NEJ tegument and parenchyma, supporting our suggestion that these enzymes are secreted during host invasion to protect the parasites from the harmful oxidative bursts produced by the activated innate immune response. By producing superoxide enzymatically in vitro, we were able to demonstrate robust killing of F. hepatica NEJ within 24 h post-excystment, and that the lethal effect of ROS was nullified with the addition of SOD and catalase (the antioxidant enzyme responsible for the dismutation of hydrogen peroxide, a by-product of the SOD reaction). This study further elucidates the mechanism by which F. hepatica protects against ROS derived from cellular metabolism and how the parasite could mitigate damage caused by the host’s immune response to benefit its survival.

Glucosamine and maltol anchored Zinc(II) complex of COVID-19 health supplement relevance: DFT collaborated spectroscopic formulation with profound biological implications

In association with other antiviral drugs, Zinc is specially administered to the patients suffering from novel coronavirus infectious disease (nCOVID). Zn, maltol, and glucosamine are famous food and drug additives. The supplements made from them are helpful in minimizing malnutrition problems, and in enhancing immune power. Due to the well-pronounced effects of all these three components in the food and medicinal industry, a novel sugar Zn(II) complex of the general composition, [Zn(gls)(mal)], where Hmal is maltol and Hgls is referred to as glucosamine, was synthesized and formulated. The physicochemical methods that were used to establish the molecular structure include elemental analysis, 1HNMR, FT-IR, UV–Vis., thermal and mass spectrometry. Physical properties like decomposition temperature and molar conductance were also examined. The experimental results at each step of characterization were validated/compared with density functionalized spectroscopic/spectrometric data using the LANL2DZ basis set for the metal atom and 6–31 g(d,p) for other atoms under the B3LYP functional. From the study, a suitable square planar geometry is suggested for the complex. Among biological implications, superoxide dismutation (SOD) and antimicrobial actions were studied. Also, virtual screening using SWISS ADME and Autodock 4.0 program (against 6X2B, SARS-CoV-2 u1S2q 2 RBD Up Spike Protein Trimer) were evaluated for the complex. Good interactions were scored by glucosamine and the complex. The results obtained from antimicrobial sensitivity indicate low inhibition zones, but from the SOD data, the complex has shown satisfactory antioxidant behavior. Therefore, the proposed food supplement could act as a good antioxidant agent and could keep the flora of the intestinal tract less disturbed while going through a metabolic pathway.

Atomically dispersed 3d metal bimetallic dual-atom catalysts and classification of the structural descriptors

Solid atomic catalysts with well-defined and complex structures are believed to effectively bridge homogeneous and heterogeneous catalysis. Nonetheless, the current limited capacity of “precise engineering” in solid atomic catalysts has led to structural heterogeneity and thus unsatisfactory catalytic selectivity. Here, we show that late 3 d metal cations, such as Co 2+ , Ni 2+ , Cu 2+ , and Zn 2+ , can be assembled to afford combinations of “dual atoms” within zeolitic micropores, and this clearly avoids issues like uncontrolled metal aggregation during synthesis. In this work, by the quantitative evaluation of the structural descriptors over a probe superoxide dismutation reaction, we demonstrate the unique synergistic advantage between (i) neighboring bimetallic active motifs, (ii) tertiary structure around the zeolitic support, and (iii) the local coordination environment. The identification and tunability of the structural descriptors shown in this work unravel a reliable approach to the precise engineering of next-generation solid dual-atom catalysts. • Atom-precise synthesis of supported bimetallic dual-atom catalysts • Reliable approach for precise microenvironment engineering in solid catalysts • Discovery of the interplay between the structural descriptors and catalytic properties • Demonstration of synergistic effect with sorafenib for augmented tumor cell apoptosis We aim to present a creative approach to afford dual-atom bimetallic catalysts in a controlled and modular manner. This directly allows the combinations of different late 3 d metals (forming M 1 -M 2 -supported dual atoms) and shows extensive possibility not limited to catalytic applications. Through the unique synergistic advantage between the two metal species and the tertiary structure around the zeolitic support, we demonstrated the feasibility of developing next-generation dual-atom bimetallic catalysts in a controlled manner as well as the identification of the structural descriptors. The manipulation and control of materials and phenomena at atomic and molecular dimensions are achieved by utilizing the fundamental concepts of coordination and solid-state chemistries. The findings presented in this work reveal the precise engineering of supported dual-atom catalysts (such as combinations of 3 d /4 d metals) over support materials not only for biochemical applications but also for more extensive applications. This shall genuinely bring heterogeneous catalysis toward the molecular frontier that addresses the critical challenges in the field.

Abstract 128: SIRT3 supports anchorage-independent survival of ovarian cancer cells

Abstract Metabolic changes during tumor progression have numerous consequences on cancer cells, including an increased risk of cell death due to production of reactive oxygen species (ROS). Antioxidant defense adaptations thus accompany metabolic alterations as essential survival mechanisms during metastasis. We observe that oxidative phosphorylation is increased when ovarian cancer (OC) cells transition to anchorage independence (a-i), an important step during metastasis in the peritoneal cavity. To combat the rise in oxidative stress resulting from this metabolic shift, a rapid increase in the sirtuin 3 (SIRT3)/superoxide dismutase 2 (SOD2) axis occurs to reduce the mitochondrial superoxide anion surge and to maintain cellular viability. We demonstrate that this upregulation occurs in transcription, translation, and SIRT3-mediated regulation of SOD2 activity. However, the fate of the hydrogen peroxide (H2O2) resulting from superoxide dismutation through SIRT3/SOD2 remains unknown. RNAseq analysis of attached versus a-i conditions revealed a significant downregulation of peroxiredoxin 3, a major scavenger of H2O2 in the mitochondria, suggesting that OCs have a net increase in mitochondrial H2O2 in a-i. At sub-lethal levels, H2O2 serves as a second messenger of redox signaling, primarily via protein cysteine oxidation, which has been implicated with metastasis. We thus hypothesize that increases in SIRT3/SOD2/H2O2 play a role as mediators of redox signaling during OC anchorage-independent survival, spheroid formation and metastasis. Using the HyPer7 redox probe and manipulation of SIRT3 and SOD2 expression, H2O2 generation and localization in a-i are being assessed using 3D fluorescence microscopy. Moreover, siRNA mediated knock-down of SIRT3 in a-i leads to the downregulation of genes involved in specific signaling pathways, including NF-kB signaling, the inflammatory response, and metabolism. Mass-spec studies are underway to identify specific SIRT3 target proteins upstream of these transcriptional changes, by assessing global cysteine oxidation and the cellular acetylome to determine the role of H2O2-dependent redox signaling and SIRT3-dependent protein de-acetylation, respectively. Given the increase in SIRT3-dependent inflammatory signaling in a-i, we are exploring how the SIRT3/SOD2/H2O2 axis influences OC interaction with tumor associated macrophages and mesenchymal stem cells, commonly found in the ascites tumor environment. This work demonstrates a multi-faceted role for the SIRT3/SOD2/H2O2 axis in a-i survival, a key step during OC metastasis. Citation Format: Priscilla Tang, Yeon Soo Kim, Piyushi Gupta-Vallur, Amal T. Elhaw, Zaineb Javed, Weihua Pan, Nadine Hempel. SIRT3 supports anchorage-independent survival of ovarian cancer cells [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2022; 2022 Apr 8-13. Philadelphia (PA): AACR; Cancer Res 2022;82(12_Suppl):Abstract nr 128.

Erythrocuprein, also Known as Superoxide Dismutase, Is a Hydroquinone Oxidase, and Imparts Resistance to Mitomycin C

The enzymatic function of superoxide dismutase (SOD) is proposed to be as a ubiquinol oxidase. The SOD activity of this protein is likely a consequence of the necessary dismutation of superoxide (O2˙ˉ), generated as an enzyme-bound intermediate during its normal activity. The relatively low specificity of this enzyme for hydroquinones allowed it to oxidize a wide range of hydroquinone substrates. The general hydroquinone oxidase activity of this enzyme would thus enable it to behave as a mammalian analogue to bacterial mitomycin C resistance protein (MCRA). This would account for its elevated activity in cells expressing resistance against mitomycin C, porfiromycin, and related analogues, since superoxide itself is relatively nontoxic.

Preventive effect of Lactobacillus plantarum HFY15 on carbon tetrachloride (CCl4 )-induced acute liver injury in mice.

Carbon tetrachloride (CCl4 ) is the main chemical causing liver damage. In this experiment, the effect of Lactobacillus plantarum HFY15 treatment on CCl4 -induced acute liver injury was investigated using mice. Fifty adult mice were randomized into five study groups, each group with 10ml kg-1 saline, 50mg kg-1 silymarin, and 109 CFU kg-1 L. plantarum HFY15 and LDSB per day, and all the mice expect the normal group were injected 0.8% CCl4 (10ml kg-1 ) on the 14th day. Following the 16 h induction of the liver injury, various biochemical markers were assessed for blood and liver tissue. After L. plantarum HFY15 treatment, the content of alanine aminotransferase (ALT), aspartate aminotransferase (AST), triglycerides (TG), malondialdehyde (MDA), and reactive oxygen species (ROS) in serum decreased by 67.7%, 65.0%, 41.9%, 59.5%, and 51.5%, respectively, and the level of antioxidant enzymes (total superoxide dismutation [T-SOD], catalase [CAT], glutathione [GSH]) increased by more than twofold. Pro-inflammatory cytokine interleukin-6 (IL-6), interferon-γ (INF-γ), and tumor necrosis factor-α (TNF-α) decreased by more than 45% in serum and live. What is more, L. plantarum HFY15 increased the expression of antiapoptosis genes Bcl-2 by eightfold, inhibiting the expression of proapoptotic genes Caspase-3 and Bax by about threefold. Lactobacillus plantarum HFY15 has obvious protective effects on CCl4 -induced liver injury by inhibiting oxidation, reducing the release of inflammatory factors, and exerting suppressive effect on apoptotic process in the CCl4 -induced liver injury. Lactobacillus plantarum HFY15 can be developed as edible lactic acid bacteria for preventing liver toxicity. PRACTICAL APPLICATION: L. plantarum HFY15 can alleviate liver injury caused by carbon tetrachloride toxicity through antioxidant, anti-inflammatory and anti-apoptotic pathways.

Wheat Grass (Triticum aestivum L.) Benefits Health in a Pandemic Scenario

This review study focused on wheat grass; an important Indian medicinal plant that has long been utilised in Ayurvedic medicine. For its healing properties, wheat grass juice includes every mineral possible along with vitamins A and B-Complex (combined). For its healing properties, wheat grass juice includes every mineral possible along with vitamins A and B-Complex (combined) (K). It includes 17 amino acids, the building blocks of protein, making it a high-protein food source. Superoxide dismutation enzymes help break down superoxide radicals in tissues and organs. Grass fed wheat seems to lower blood glucose levels. According to study, wheat grass juice improves general health by increasing strength, endurance, and overall happiness. Wheat grass juice consumption is widespread in both urban and rural areas. Thus, it is vital to study the therapeutic advantages of these active components present in young wheat grass on both healthy and COVID-19 affected patients to cure diseases by boosting the immune system and preventing infection and disease.