Superficial Topography Research Articles

Optimising surface morphology for enhanced radiative properties in thermal energy-efficient materials

Environmental concerns related to heat-absorbing materials in the construction industry can be effectively addressed by optimising the reflectivity of incoming sunlight and thermal emissivity of materials. These radiative properties are closely tied to the surface morphology characteristics of building materials, making superficial topography a critical factor in determining their thermal and energy performance. In our research, we performed reflectance analysis in the UV-Vis-NIR-MIR region and conducted FT-IR analysis to assess the colour and reflectance behaviour of the samples, along with surface morphology and topography investigations. The data collected were utilised in a statistical study to analyse the relationship between superficial topography and thermo-optical characteristics. Our findings underscore the significant impact of surface morphology as a secondary factor influencing radiative properties. Specifically, our results indicate that large-scale components influence mid-infrared absorbance, while surface roughness is crucial in determining solar reflectance properties. Notably, an increase in roughness consistently leads to a reduction in near-infrared and visible reflectance properties by up to 17% and 8%, respectively, with no noticeable effect in the UV region. In conclusion, our research demonstrates the potential for customising and optimising surface properties to reduce energy consumption for cooling purposes and alleviate the urban heat island effect.

Investigating the relationship between surface roughness and reflectance properties of building materials

Materials used in the exterior envelope of buildings and open urban surfaces, in general, strongly affect the urban thermal balance, determining the general magnitude of urban overheating. The surface temperature of reflective materials varies as a function of physical and geometrical properties. Quantifying the influence of surface roughness on reflectance properties has crucial relevance since reflectance can significantly affect the reduction of the absorbed solar radiation and, in turn, the energy demand for cooling. Through an experimental and statistical investigation, this research aims to analytically assess the impact of surface roughness on the reflectance and thermal performances of building materials and, in turn, the role that roughness could play towards urban cooling and mitigation. Results show that the surface’s different roughness affects the sample’s reflectance coefficient, leaving it basically unchanged in the Ultraviolet and Visible ranges but with appreciable differences in the Near-Infrared wavelengths. This outcome confirms the correlation between the surface roughness and the optical and thermal characteristics of the building materials, making evident the importance of the study of superficial topography towards the mitigation of Urban Heat Island phenomena.

Nanoporous Titanium Enriched with Calcium and Phosphorus Promotes Human Oral Osteoblast Bioactivity.

Implant surfaces are known to influence the osseointegration process; therefore, their modifications represent an important subject of investigation. On this basis, the purpose of this study was to evaluate the response of human oral osteoblasts (hOBs) to three different GR4 titanium discs: Machined, double-etched (Osteopore), and double-etched, surface-enriched with calcium and phosphorus (CaP) (Nanopore). The superficial topography was investigated with scanning electron microscopy (SEM) and the sessile drop technique. To test cellular response and osteoinductive properties, the following points were evaluated: (i) proliferation by MTS assay after 2 and 5 days; (ii) adhesion by multiphoton microscopy at day 2; (iii) the interaction with Ti discs by blue toluidine staining at day 5; (iv) alkaline phosphatase (ALP) activity by ALP assay after 14 days; (v) calcium deposition by alizarin red staining and by cetylpyridinium chloride after 14 days. The SEM analysis showed that Nanopore and Osteopore surfaces were characterized by the same micro-topography. Nanopore and Osteopore discs, compared to Machined, stimulated higher osteoblast proliferation and showed more osteoinductive properties by promoting the ALP activity and calcium deposition. In conclusion, the CaP treatment on DAE surfaces seemed to favor the oral osteoblast response, encouraging their use for in vivo applications.

Biocompatibility and antibiofilm activity of graphene-oxide functionalized titanium discs and collagen membranes

The aims of the study were: 1) to evaluate the effect on biofilm formation of barrier membranes and titanium surfaces coated with graphene-oxide (GO); 2) to analyze the connection between the superficial topography of the tested materials and the amount of bacterial accumulation on them and 3) to analyze the biocompatibility of GO functionalized discs using the zebrafish model. MethodsSingle species bacterial biofilms (Streptococcus oralis, Veilonella parvula, Fusobacterium nucleatum, Porphyomonas gingivalis) were grown on GO-free membranes, membranes coated with 2 and 10 μg/ml of GO, GO-free and GO-coated titanium discs. The biofilms were analyzed by determining the CFU count and by Scanning Electron Microscopy (SEM) and the materials’ topography by Atomic Force Microscopy (AFM). Zebrafish model was used to determine the materials’ toxicity and inflammatory effects. ResultsAFM showed similar roughness of control and GO-coated materials. CFU counts on GO-coated discs were significantly lower than on control discs for all species. CFU counts of S. oralis, V. parvula and P. gingivalis were lower on biofilms grown on both types of GO-coated membranes than on GO-free membrane. SEM analysis showed different formation of single species biofilm of S. oralis on control and GO-coated materials.GO-functionalized titanium discs do not induce toxic or inflammatory effects. SignificanceTitanium implant surfaces functionalized with GO have shown to be biocompatible and less susceptible to biofilm formation. These results encourage further in vivo investigation of the tested materials on infection prevention, specifically in prevention and reduction of peri-implant mucositis and periimplantitis incidence.

Profilometer Comparison of the Surface Roughness of Four Denture Base Resins: An In Vitro Study

Background: The roughness of denture base materials is mainly affected by inherent material features, the polishing technique, and the operator’s manual skills. The surface irregularities of the denture base resin’s roughness profile is one of the components characterizing the superficial topography of dental prostheses, and it is a critical parameter for describing surface morphology. Generally, the increment of the surface roughness minimizes cleanability and promotes the rate of bacterial adherence, affecting the expression of bacterial adhesins. The purpose of this in vitro study was to investigate the roughness of four commercially available denture base resins employed for removable prosthodontics. Methods: Twenty-five specimens were realized and submitted to observation for three dimensions (vertically, horizontally, 45°). Average surface roughness (Ra), Rv, Rp, and Rq were measured with a calibrated mechanical roughness tester (Taylor Hobson Surtronic 25; Taylor Hobson, Leicester, UK). Data were analyzed through a Kruskal–Wallis test. Results: Significant differences in Ra between the groups were assessed. Baltic Denture System™ (Merz Dental GmbH; Lütjenburg, Germania) showed a lower surface roughness coefficient and a more homogeneous surface. Conclusions: The functional importance of surface roughness parameters for denture base materials must be explored, not only considering recognized surface features.

Injection-type Denture Base Materials Surface Modification after Vapor Plasma Deposition

The purpose of this study was to investigate changes of superficial topography and wettability of two injection-type denture base materials following low pressure plasma treatment. Samples of denture base materials (Polyan and Biodentaplast) were fabricate using dedicated technology and were exposed to plasma treatment. Resin surface topography and rugosity were evaluated using SEM and AFM, while wettability was determined through contact angle measurements. Artificial saliva was the testing liquid. Initial contact angles for the two materials are close (Biodentaplast-37.60�, Polyan-36.75�). Plasma treatment halves the values of the contact angle. 30-days measurement reveals a reduced bounce-back effect (Biodentaplast-20.68�, Polyan-20.11�). Surface topography modified differently for the two materials. Rugosity increased significantly for both materials (p[0.05). Surface rugosity values pre- and post-plasma treatment respect the biological threshold of fungal adhesion. Plasma exposure increased injection-type denture base materials wettability with artificial saliva and surface roughness. Injection-type denture base materials and artificial saliva can enhance prosthetic experience of xerostomic patients.

The Bacterial Anti-Adhesive Activity of Double-Etched Titanium (DAE) as a Dental Implant Surface.

This work aimed to compare the capability of Streptococcus oralis to adhere to a novel surface, double-etched titanium (DAE), in respect to machined and single-etched titanium. The secondary outcome was to establish which topographical features could affect the interaction between the implant surface and bacteria. The samples’ superficial features were characterized using scanning electron microscopy (SEM) and energy dispersive x-ray spectrometry (EDS), and the wetting properties were tested through sessile methods. The novel surface, the double-etched titanium (DAE), was also analyzed with atomic force microscopy (AFM). S. oralis was inoculated on discs previously incubated in saliva, and then the colony-forming units (CFUs), biomass, and cellular viability were measured at 24 and 48h. SEM observation showed that DAE was characterized by higher porosity and Oxygen (%) in the superficial layer and the measurement of the wetting properties showed higher hydrophilicity. AFM confirmed the presence of a higher superficial nano-roughness. Microbiological analysis showed that DAE discs, coated by pellicle’s proteins, were characterized by significantly lower CFUs at 24 and 48 h with respect to the other two groups. In particular, a significant inverse relationship was shown between the CFUs at 48 h and the values of the wetted area and a direct correlation with the water contact angle. The biomass at 24 h was slightly lower on DAE, but results were not significant concerning the other groups, both at 24 and 48 h. The DAE treatment not only modifies the superficial topography and increased hydrophilicity, but it also increases the Oxygen percentage in the superficial layer, which could contribute to the inhibition of S. oralis adhesion. DAE can be considered a promising treatment for titanium implants to counteract a colonization pioneer microorganism, such as S. oralis.

Influence of surface treatments in flexural strength and superficial topography of a lithium disilicate ceramic

The aim of the study was to evaluate the influence of different surface post-etching treatments in flexural strength, contact angle and surface roughness of a lithium disilicate ceramic. 60 bars (16x2x4mm) were divided in six groups (n=10): C, no treatment; HF, hydrofluoric acid for 20s (HF) + washing (60s), drying (30s); HFL, HF + washing (60s), drying (30s) and luting; HFNL, HF + washing (60s) + neutralization with sodium bicarbonate (SB) for 40s + washing (5s), drying (30s) and luting; HFUL, HF + washing (60s) + ultrasonic bath (UB) for 4 min, drying (30s) and luting; HFNUL, HF + washing (60s) + SB (40s) + UB (4 min) and luting. The bars were submitted to three-points flexural strength test. To analyze roughness and contact angle 25 ceramic blocks (12x7x7 mm) were divided into five groups (n=5): C - no treatment; HF - HF ; HFU - HF and UB for 5 min ; HFN - HF and SB; HFNU - HF, UB and SB. A scanning electron microscope (SEM) analysed the surface of the samples. ANOVA one-way statistical analysis was done for comparison of results. There was no statistical difference for flexural strength (p-value=0.15) and for surface roughness (p-valor=0.15). However, it was obtained statistically significant difference for contact angle (p-valor=0.00). SEM images showed precipitates after the acid etching, which were removed by post-etching treatments. It can be concluded that the different post-etching surface treatments did not increase the flexural strength, surface roughness and ceramic wetting, but it influenced the SEM images.

Effects of ultrasonic scaling on the optical properties and surface characteristics of highly translucent CAD/CAM ceramic restorative materials: An in vitro study

Abstract Esthetic restorations using highly translucent ceramics powered by chairside digital dentistry are becoming popular. The purpose of this in vitro study was to investigate the impact of ultrasonic scaling on the optical and surface properties of highly translucent ceramic materials for digital dentistry. A resin nanoceramic (Lava Ultimate; LU), dual-network ceramic (Vita Enamic; VE), feldspathic ceramic (Vitablocs Mark II; VM), lithium disilicate ceramic (IPS e.max CAD; EX), and high-translucency monolithic zirconia (Rainbow Shine-T; MZ) were evaluated. All specimens were subjected to ultrasonic scaling, and the following data were obtained before and after scaling: color change (Δ E 00 ), translucency parameter, surface gloss, surface roughness, and superficial topography. One-way analysis of variance (ANOVA), repeated-measures ANOVA, and two-way ANOVA were used for intergroup comparisons (all α = 0.05). The mean Δ E 00 values were 0.243, 0.48, 1.591, 0.143, and 4.466 for LU, VE, VM, EX, and MZ, respectively, with statistically significant differences among the materials. With regard to Commission Internationale de l'Eclairage (CIE) L* , a* , and b* values, VE, VM, and MZ showed significantly decreased L* values relative to the baseline values. Moreover, MZ showed a significantly increased a* value and a significantly decreased b* value after scaling. Ultrasonic scaling also resulted in significant changes in the surface gloss of the LU, VE, VM, and MZ specimens. Micrographs showed scrapes and surface deterioration after scaling. For all materials, the translucency parameter and the surface roughness showed no significant differences between specimens that were subjected to scaling and those that were not. These findings suggest that ultrasonic scaling MARKEDLY affects the optical properties and surface characteristics of highly translucent computer-aided design and computer-aided manufacturing (CAD/CAM) ceramics. The findings can aid restorative dentists in the selection of appropriate materials and motivate periodontists for performing scaling procedures with due consideration of restorations in esthetically demanding areas.

Optical and Surface Properties of Monolithic Zirconia after Simulated Toothbrushing.

This in vitro study investigated the impact of various dentifrices on the shade, translucency, gloss, and surface characteristics of polishing- or glazing-finished monolithic zirconia surfaces after simulated toothbrushing. Eighty square-shaped monolithic zirconia specimens were divided into two major groups based on the finishing methods—polished (P) and glazed (G). Next, specimens from the two major groups were categorized into four subgroups: stored in distilled water (DW, control); brushed with a fluoride-free conventional dentifrice (C); brushed with a fluoride dentifrice (F); and brushed with a whitening dentifrice (W). Overall, eight groups were created—PDW, PC, PF, PW, GDW, GC, GF, and GW (n = 10 each). Shade, translucency, surface gloss, surface roughness, crystalline phase, and superficial topography data were obtained. Repeated-measures ANOVA and two-way ANOVA were used for intergroup comparison (all α = 0.05). The color differences (ΔE00) between pre- and posttreatment were 0.3158 (PDW), 0.7164 (PC), 0.7498 (PF), 0.8106 (PW), 0.1953 (GDW), 0.301 (GC), 0.3051 (GF), and 0.4846 (GW). A statistically significant difference was observed among the ΔE00, surface gloss, and surface roughness of monolithic zirconia. Thus, brushing with several dentifrices markedly affects the optical properties and surface characteristics of monolithic zirconia finished with polishing or glazing methods.

Effects of whitening dentifrice on yttria-stabilized tetragonal zirconia polycrystal surfaces after simulating brushing

Statement of problemThe changes that occur after brushing yttria-stabilized tetragonal zirconia polycrystal (Y-TZP) are unknown. These changes may favor the retention of microorganisms and chemisorption of water, impairing its longevity. ObjectiveThe purpose of this in vitro study was to evaluate the effects of a whitening dentifrice on Y-TZP surfaces after simulating 10 years of brushing. Material and methodsSeventy-two bar-shaped specimens (20×4×1.2 mm) were divided into 4 groups: storage in distilled water (SW, control), brushing with distilled water (BW), brushing with dentifrice (BD), and brushing with whitening dentifrice (BWD). Brushing was conducted using a linear brushing machine (878400 cycles, 0.98 N, soft toothbrush). The mean roughness (Ra) was analyzed with a profilometer and the superficial topography with scanning electron microscopy (SEM) at baseline and after treatment. Crystalline phases were characterized using x-ray diffraction. Baseline and posttreatment Ra were analyzed using the 1-way ANOVA and Tukey HSD multiple comparison test; the paired t test was used for intragroup comparison (all α=.05). ResultsThe Ra (μm) means (before/after treatment) were SW 0.28/0.28; BW 0.32/0.31; BD 0.28/0.36; BWD 0.30/0.20. No statistically significant difference was found for Ra at baseline (P=.108) than for posttreatment results (P<.001); the BD group had higher Ra values when compared with baseline (P=.019); the BWD group had the lowest values (P<.001). The BD surfaces showed pronounced scratches and detachment of the surface, while BWD showed smoother surfaces; similar crystallographic results among groups were observed. ConclusionsBrushing Y-TZP with conventional dentifrice increased roughness, while brushing with whitening dentifrice reduced roughness. Neither dentifrice changed the crystallographic phases after brushing.

Induction of hepatocyte functional protein expression by submicron/nano-patterning substrates to mimic in vivo structures

To investigate the influence of bio-inspired metallic superficial topography on the cellular behaviour of a hepatocyte cell line, TiO2 nanopatterns with diversified shapes and heterotropic lateral dimensions were fabricated using electron beam lithography and atomic layer deposition. The dimensional uniformity and shape diversity of the nanopatterns were confirmed using scanning electron microscopy and atomic force microscopy. These topographical nanocues provide good tools for controlling and regulating multiple hepatocellular functions. The expressions of functional proteins such as albumin, transferrin and cytochrome P450 were tested as functional markers. In addition, the change in cellular orientation, cell alignment and native extracellular matrix (ECM) assembly induced by these well-defined nanotopographies were observed. Twelve hours after cell seeding, TiO2 nanogratings with a lateral dimension of 240 nm showed a higher degree of functional protein expression compared to other nanotopographical substrates and a flat surface. These findings suggest that the TiO2 surface resembles a hierarchically-extended collagen nanofibrillar surface and could be recognized by hepatocytes, allowing the proper cytoskeletal orientation and cellular integrity. This TiO2 nanopattern with a specific shape and dimension (240 nm) might therefore emulate ECM biophysical cues, and the intrinsic topography of TiO2 surfaces might evoke enhanced cellular responses. These unique surfaces could be further exploited for tissue engineering and bioreactor technology.

Brain arteriovenous malformations and seizures: an Italian study

ObjectiveTo evaluate seizures as first clinical manifestation of brain arteriovenous malformations (AVMs), in relation to angioarchitectural features of these vascular anomalies.MethodsWe performed a prospective observational study, collecting records of patients...

Replication of Micro Laser Textures by Injection Molding

Increasingly micro technology becomes more important in order to develop new products with high added value. These new technologies known as micro allow us manufacturing precision components; these new micro components should work and take carrying out the functions previously performed by larger parts. Microinjection is one of these new technologies. This has the capacity to produce parts, for different materials both plastic and metal and for some industries and applications. The main objective in this paper is to determine the replicate microtextures capability for plastic injection molds. For our samples, ABS plastic is injected into four aluminum cavities with different laser textures performed in, using different technologies to get them. In order to analyze how mold texture affects parts, optical interferometry technique was selected to measure it. The superficial topography obtained was processed using MountainsMapTM software, in order to get the replicability of injected parts. It has also been used an electron microscopy (SEM) to evaluate the mold textures and injected parts in a photographically way.

Synergistic responses of superficial chemistry and micro topography of titanium created by wire-type electric discharge machining

Wire-type electric discharge machining has been applied to the manufacture of endosseous titanium implants as this computer associated technique allows extremely accurate complex sample shaping with an optimal micro textured surface during the processing. Since the titanium oxide layer is sensitively altered by each processing, the authors hypothesized that this technique also up-regulates biological responses through the synergistic effects of the superficial chemistry and micro topography. To evaluate the respective in vitro cellular responses on the superficial chemistry and micro topography of titanium surface processed by wire-type electric discharge, we used titanium-coated epoxy resin replica of the surface. An oxide layer on the titanium surface processed by wire-type electric discharge activated the initial responses of osteoblastic cells through an integrin-mediated mechanism. Since the mRNA expression of ALP on those replicas was up-regulated compared to smooth titanium samples, the micro topography of a titanium surface processed by wire-type electric discharge promotes the osteogenic potential of cells. The synergistic response of the superficial chemistry and micro topography of titanium processed by wire-type electric discharge was demonstrated in this study.

Effect of fluid resins on the surface roughness and topography of resin composite restorations analyzed by atomic force microscope

The aim of the study was to verify the influence of surface sealants on the surface roughness of resin composite restorations before and after mechanical toothbrushing, and evaluate the superficial topography using atomic force microscope. Five surface sealers were used: Single Bond, Opti Bond Solo Plus, Fortify, Fortify Plus and control, without any sealer agent. The lowest values of surface roughness were obtained for control, Single Bond and Fortify groups before toothbrushing. Fortify and Fortify Plus were the sealer agents that support the abrasive action caused by the toothbrushing although Fortify Plus group remained with high values of surface roughness. The application of specific surface sealants could be a useful clinical procedure to maintain the quality of resin-based composite restorations.

Superficial Topography and Porosity of an Absorbable Barrier Membrane Impacts Soft Tissue Response in Guided Bone Regeneration

Surface topography and porosity of barrier membranes is suggested to impact the soft and hard tissue response. In this study, the specific soft and hard tissue response characteristics of a synthetic polylactide membrane are evaluated including soft tissue inflammation, osteogenesis, and osteopromotion. Analysis of porosity and surface topography of the test material was performed by scanning electron microscopy. Transosseous parietal defects were surgically created bilaterally in 32 BalbC/ByJ mice and treated either with the barrier (test) or sham-operated (control). Healing was assessed histologically and histomorphometrically with quantification of bone bridging. Scanning electron microscopy analyses of the barrier revealed a microstructure resembling cancellous bone. Interconnecting pores and channels, measuring between 6 and 60 microm in diameter, formed by smooth internal walls were observed throughout the device. Two distinct patterns of porosity were observed. The external surface of the membrane was characterized by a highly porous structure, with minimal interporous nodes and average pore sizes ranging between 6 and 20 microm in diameter. The internal surface was characterized by a minimal porous structure, with significant interporous nodes and average pore sizes ranging between 18 and 60 mum in diameter. Histomorphometric analyses demonstrated increased bone bridging by 60% and 300% in membrane-treated sites after 14 and 28 days of healing, respectively. The rough surface of the barrier contained significantly more giant cells, whereas the smooth surface contained significantly more inflammatory cells. The surface topographies engineered on different sides of the barrier promote differential soft tissue responses leading, however, to similar amounts of enhanced bone formation.

Experimental analysis and numerical modelling of the forming process of polypropylene replicas of micro-cavities using hot embossing

Numerical modelling of the deformation of a polymer using the finite elements method in axisymetrical mode was performed using the LsDyna® software to describe the filling of micro-cavities during the forming process of the material using the hot embossing. These simulations firstly allow verifying whether the chosen forming process conditions promote or not an optimized filling of the superficial cavities in order to achieve precise replicas which best reproduce the superficial topography of the mould. The simulations were carried out to evaluate the filling of the cavities taking into account the mechanical behaviour of the selected polymer into the model. Moreover, these models were developed to verify the effect of the distribution of the mould cavities on their filling. The influence of the mobility of non deformable rigid plates on the filling of the cavities represents an auxiliary variable. In the approach presented, the compression plates are assumed to be parallel and non deformable, whereas the polymer disk follows a rubbery behaviour around a temperature equal to 140°C. Globally the modelling results are satisfactory for they are rather close to the experimental observations conducted. In summary, the effect of the normal stress as also the distribution of micro-cavities at the mould surface seem to prevail in the case of the forming process by hot embossing.

SEM analysis of enamel surface treated by Er:YAG laser: Influence of irradiation distance

Depending on the distance of laser tip to dental surface a specific morphological pattern should be expected. However, there have been limited reports that correlate the Er:YAG irradiation distance with dental morphology. To assess the influence of Er:YAG laser irradiation distance on enamel morphology, by means of scanning electron microscopy (SEM). Sixty human third molars were employed to obtain discs (approximately =1 mm thick) that were randomly assigned to six groups (n=10). Five groups received Er:YAG laser irradiation (80 mJ/2 Hz) for 20 s, according to the irradiation distance: 11, 12, 14, 16, or 17 mm and the control group was treated with 37% phosphoric acid for 15 s. The laser-irradiated discs were bisected. One hemi-disc was separated for superficial analysis without subsequent acid etching, and the other one, received the phosphoric acid for 15 s. Samples were prepared for SEM. Laser irradiation at 11 and 12 mm provided an evident ablation of enamel, with evident fissures and some fused areas. At 14, 16 and 17 mm the superficial topography was flatter than in the other distances. The subsequent acid etching on the lased-surface partially removed the disorganized tissue. Er:YAG laser in defocused mode promoted slight morphological alterations and seems more suitable for enamel conditioning than focused irradiation. The application of phosphoric acid on lased-enamel surface, regardless of the irradiation distance, decreased the superficial irregularities.

Superficial topography of wound: A determinant of underlying biological events?

Three-dimensional configuration of wounds varies considerably according to the etiology. Wounding of skin is proceeded by release of dermal pretension. Subsequent disruption of physical equilibrium with resulting development of force vectors alters the primary shape of wound to maintain a new dynamic physical equilibrium. This leads to the development of stress-relaxation and stress-concentration areas throughout the wound milieu. Mechanical strain produces piezoelectric current which is maximal in stress-relaxation regions due to lower tissue stiffness and higher mobility. Early surge in the tissue level of TGF-beta would be exaggerated through synergistic interaction with piezoelectric current in stress-relaxation areas. Subsequently, fibroblasts migrate to these areas due to galvanotaxis. The gradual dissipation of tissue tension, due to irreversible loss of viscous strain, reduces the synergistic action of TGF-beta and piezoelectricity. However, a similar pattern of activity of TGF-beta due to the polarized migration of fibroblasts, which are the main source of TGF-beta during secondary surge, may be continued. It seems that a biological-mechanical continuum exists for wounds so that even the superficial topography of wounds may affect the underlying biological activity and final healing outcome during healing of dermal wounds.