Superparamagnetic Nanoparticles Research Articles

Engineering Zn/Fe Mixed Metal Oxides with Tunable Structural and Magnetic Properties for Magnetic Particle Imaging

Engineering magnetic nanoparticles with tunable structural properties and magnetism is critical to develop desirable magnetic particle imaging (MPI) tracers for biomedical applications. Here we present a new superparamagnetic metal oxide nanoparticle with a controllable chemical composition and magnetism for imaging tumor xenografts in living mice. Superparamagnetic Zn/Fe mixed metal oxide (ZnFe-MMO) nanoparticles are fabricated via a facile one-pot co-precipitation method in water followed by thermal decomposition with tunable Zn/Fe ratios and at various calcination temperatures. This work, for the first time, presented LDH-derived metal oxides for an MPI application. The metal composition is tunable to present an optimized MPI performance. The analytical results demonstrate that ZnFe-MMO nanoparticles at the designed molar ratio of Zn/Fe = 2:1 after 650 °C calcination demonstrate a higher saturation magnetization (MS) value and optimal MPI signal than the samples presented with other conditions. The excellent biocompatibility of ZnFe-MMO is demonstrated in both breast cancer cells and fibroblast cell cultures. In vivo imaging of 4T1 tumor xenografts in mice using ZnFe-MMO as a tracer showed that the mean signal intensity is 1.27-fold higher than the commercial tracer VivoTrax at 72 h post-injection, indicating ZnFe-MMO’s promise for prolonged MPI imaging applications.

Vortex structure and control in a lid-driven cavity with magnetic field

Ferrofluids are magnetic field-sensitive colloidal solutions of ultra-fine, single-domain superpara-magnetic nanoparticles of metallic materials. An external magnetic field can be used to regulate the magnetic nanoparticles in the ferrofluid, which permits the fluid to flow and distribute within the domain in a predetermined way. This study examines the ferrofluid flow properties in a lid-driven cavity exposed to an external magnetic field. We investigate the impact of different aspect ratios, Reynolds number, and magnetic field strength on generation of vortices and their behavior. We have simulated aspect ratios of 1, 0.5, and 2 in this investigation, using Reynolds number values of 100, 400, and 1000 and magnetic field strength of 0, 1, and 4 tesla. Our results show that the introduction of magnetic fields greatly modifies the behavior of vortex, affecting the flow stability, and vorticity patterns inside the cavity. These findings have ramifications for actuation mechanisms, drug delivery systems, and microfluidic devices. In particular, we find that the flow patterns in the cavity evolve from basic recirculation to more intricate vortex structures. The commencement of these transitions is largely dependent on the Reynolds numbers. Along the sidewalls, secondary vortices form at higher Reynolds numbers. Practical applications can benefit from the enhanced mixing provided by these sidewall vortices. Additionally, we talk about how ferrofluids might improve fluid control and manipulation in constrained geometries. The interaction of magnetism and fluid dynamics creates opportunities for new types of actuation mechanisms in which the ferrofluid flow can be steered and controlled by magnetic fields. Our research opens up new avenues for creative applications in microfluidics and other fields by providing insightful information on ferrofluid behavior.

Concanavalin A-activated magnetic nanoparticles as an affine material for urinary exosome isolation.

Exosomes are a type of membrane vesicle secreted into the extracellular medium by most cell types. They have a great potential for clinical practice as noninvasive biomarkers for diagnosis of various diseases, prognosis, and monitoring of therapy, which stimulates the development of simple methods for isolating exosomes from biological fluids. A novel affine material based on aminosilanized superparamagnetic core‒shell nanoparticles for fast isolation of urinary exosomes is reported. Iron oxide nanoparticles coated with amino organosilane have been synthesized. The structural and magnetic characteristics of the resulting nanoparticles have been studied by transmission electron microscopy and ferromagnetic resonance. The surface of the synthesized nanoparticles has been chemically functionalized with lectin (concanavalin A), and the efficiency of the obtained material as a sorbent for affine exosome isolation from human urine has been demonstrated. A highly purified fraction of exosomes 90-200nm in size has been obtained. The exosomal nature of the isolated vesicles has been confirmed by bioluminescent solid-phase microassay of tetrasporine receptor markers. The presence of exosomal miR-21 in the isolated human urine exosome samples has been established.

NiFe2O4@SiO2 superparamagnetic nanoparticles as contrast agents in image-guided adaptive brachytherapy (IGABT)

Focusing on the study of superparamagnetic iron oxide samples for applications as contrast agents, the potential utility of NiFe2O4@SiO2 sample as a contrast agent in image guided adapted brachytherapy (IGABT) treatments is described here. This magnetic sample was prepared by controlled hydrothermal synthesis with urea addition and further TEOS reaction using the Stöber method. X-ray patterns show diffraction maxima indexed to a cubic symmetry of S. G. Fd-3m with Z = 8, compatible with an inverse spinel-type structure. Polyhedral shape particles with a size of about 21 ± 5 nm, together with a homogeneous silica coating of 12.7 ± 1 nm thickness were observed in TEM images Catheters and plastic needles containing NiFe2O4@SiO2 sample in agarose solution mixture were investigated. Images obtained by magnetic resonance imaging (MRI), computed tomography (CT) and X-ray of the ovoid applicator containing NiFe2O4@SiO2 sample in agarose solution mixture, suggest that this sample may be useful for visualizing the source path by performing a T2 weighted MRI and without the need to acquire a CT image. This process reduces uncertainties due to patient movements between imaging modalities as well as procedure time. MRI and CT images of the interstitial needles filled with this solution at different concentrations confirm that it is also suitable for use as a contrast agent. Finally, the investigated sample-agarose mixture was also inserted into a block of human-like pork tissue to approximate an “in vivo” experimentation. The results suggest that the studied sample may be useful in IGABT treatments and intraoperative BT where a series of catheters are implanted into the surgical cavity. Currently, the contrast agents used are not visible inside plastic needles. Our study shows that no toxicity is introduced into the system since the sample is contained within plastic catheters and never encounters the tissue.

Multifunctional pure and Sr-doped magnetite superparamagnetic nanoparticles: Synthesis, characterization, magnetic heating performance, and numerical simulation

Magnetic hyperthermia based on the utilization of multifunctional superparamagnetic nanoparticles (SNPs) is a promising method for non-invasive localized cancer treatment. Understanding the influence of properties and morphology of magnetic core and surface coatings on heating performance is crucial for their utilization in clinical applications.Multifunctional Pure and Sr-doped Fe3O4 SNPs were synthesized and coated with oleic acid, silica coatings, and surface amination for modification of their surfaces. Materials characterizations were performed via FTIR, XRD, TEM/EDS microscopy, and VSM measurements. Experimental studies were conducted to assess the heating performance of these nanoparticles using nanoparticle/agarose gel composite samples. Temperature changes on their top surface over time, induced by an externally applied magnetic field, were monitored.The simulation studies in conjunction with the experimental results, were successfully employed to comparatively evaluate the effects of multiple process parameters and to gain insights into the effects of relevant multiple parameters; including the types, properties, concentrations, and distributions of SNPs; the physical properties of the organic matrix and the surrounding medium; the interface between SNPs and the matrix; as well as the geometry of the sample and the strength and geometry of the magnetic field on the heating performance of the nanoparticles.

Synthesis of dumbbell-like heteronanostructures encapsulated in ferritin protein: Towards multifunctional protein based opto-magnetic nanomaterials for biomedical theranostic

Dumbbell-like hetero nanostructures based on gold and iron oxides is a promising material for biomedical applications, useful as versatile theranostic agents due the synergistic effect of their optical and magnetic properties. However, achieving precise control on their morphology, size dispersion, colloidal stability, biocompatibility and cell targeting remains as a current challenge. In this study, we address this challenge by employing biomimetic routes, using ferritin protein nanocages as template for these nanoparticles’ synthesis. We present the development of an opto-magnetic nanostructures using the ferritin protein, wherein gold and iron oxide nanostructures were produced within its cavity. Initially, we investigated the synthesis of gold nanostructures within the protein, generating clusters and plasmonic nanoparticles. Subsequently, we optimized the conditions for the superparamagnetic nanoparticles synthesis through controlled iron oxidation, thereby enhancing the magnetic properties of the resulting system. Finally, we produce magnetic nanoparticles in the protein with gold clusters, achieving the coexistence of both nanostructures within a single protein molecule, a novel material unprecedented to date. We observed that factors such as temperature, metal/protein ratios, pH, dialysis, and purification processes all have an impact on protein recovery, loading efficiency, morphology, and nanoparticle size. Our findings highlight the development of ferritin-based nanomaterials as versatile platforms for potential biomedical use as multifunctional theranostic agents.

Open-loop narrowband magnetic particle imaging based on mixed-frequency harmonic magnetization response.

Magnetic particle imaging (MPI), a radiation-free, dynamic, and targeted imaging technique, has gained significant traction in both research and clinical settings worldwide. Signal-to-noise ratio (SNR) is a crucial factor influencing MPI image quality and detection sensitivity, and it is affected by ambient noise, system thermal noise, and the magnetization response of superparamagnetic nanoparticles. Therefore to address the high amplitude system and inherent thermal noise present in conventional MPI systems is essential to improve detection sensitivity and imaging resolution. This study introduces a novel open-loop, narrow-band MPI signal acquisition system based on mixed-frequency harmonic magnetization response. Allowing superparamagnetic nanoparticles to be excited by low frequency, high amplitude magnetic fields and high frequency, low amplitude magnetic fields, the excitation coil generates a mixed excitation magnetic field at a mixed frequency of 8.664 kHz (f H + 2f L ), and the tracer of superparamagnetic nanoparticles can generate a locatable superparamagnetic magnetization signal with rich harmonic components in the mixed excitation magnetic field and positioning magnetic field. The third harmonic signal is detected by a Gradiometer coil with high signal-to-noise ratio, and the voltage cloud image is formed. The experimental results show that the external noise caused by the excitation coil can be effectively reduced from 12 to about 1.5 μV in the imaging area of 30 mm × 30 mm, which improves the stability of the detection signal of the Gradiometer coil, realizes the detection of high SNR, and makes the detection sensitivity reach 10 μg Fe. By mixing excitation, the total intensity of the excitation field is reduced, resulting in a slight improvement of the resolution under the same gradient field, and the spatial resolution of the image reconstruction is increased from 2 mm under the single frequency excitation (20.7 kHz) in the previous experiment to 1.5 mm under the mixed excitation (8.664 kHz). These experimental results highlight the effectiveness of the proposed open-loop narrowband MPI technique in improving signal detection sensitivity, achieving high signal-to-noise ratio detection and improving the quality of reconstructed images by changing the excitation magnetic field frequency of the excitation coil, providing novel design ideas and technical pathways for future MPI systems.

Combination of magnetic hyperthermia and gene therapy for breast cancer.

This study presented a novel breast cancer therapy model that uses magnetic field-controlled heating to trigger gene expression in cancer cells. We created silica- and amine-modified superparamagnetic nanoparticles (MSNP-NH2) to carry genes and release heat under an alternating current (AC) magnetic field. The heat-inducible expression plasmid(pHSP-Azu) was designed to encode anti-cancer azurin and was delivered by magnetofection. MCF-7 cells demonstrated over 93% cell viability and 12% transfection efficiency when exposed to 75µg/ml of MSNP-NH2, 3µg of DNA, and PEI at a 0.75 PEI/DNA ratio (w: w), unlike non-tumorigenic cells (MCF-10A). Magnetic hyperthermia (MHT) increased azurin expression by heat induction, leading to cell death in dual ways. The combination of MHT and heat-regulated azurin expression induced cell death, specifically in cancer cells, while having negligible effects on MCF-10A cells. The proposed strategy clearly shows that simultaneous use of MHT and MHT-induced azurin gene expression may selectively target and kill cancer cells, offering a promising direction for cancer therapy.

Bacteriophage-linked superparamagnetic nanoparticle for sensitive and simple detection of pathogenic Escherichia coli O157:H7 bacteria

An easy-to-use and highly-sensitive magnetic nanoprobe to detect veratoxin-producing Escherichia coli O157:H7 was developed. The bacteriophage that targets E. coli O157:H7 was chemically linked to chitosan-coated iron oxide nanoparticles (5–50 nm) to form a nanoprobe for the detection of E. coli O157:H7 in aqueous samples. The nanoprobes had a limit of detection of 2 log CFU/mL in samples with pH from 5 to 8 at 25 °C, and temperatures from 25 to 40 °C at pH 7. In the beef and cabbage liquid extracts, the nanoprobe had a detection limit of 2 log CFU/mL at 25 °C and pH 7. The nanoprobe was highly specific and retrieved only E. coli O157:H7 from a sample of bacterial mixture.Gram staining method was used with the nanoprobe to visualize the bacterial cells captured from bacterial suspensions at 7 pH and 25 °C. Visualization of the bacterial cells captured from suspensions with a concentration of 2 log CFU/mL was achieved.To detect the presence of bacteria, the use of the magnetic nanoprobes and Gram staining took less than 30 min to complete. The system developed in this study offers a fast and operator-friendly alternative for highly-specific bacterial detection.

Responsive Magnetic Particle Imaging Tracer: Overcoming "Always-On" Limitation, Eliminating Interference, and Ensuring Safety in Adaptive Therapy.

Magnetic particle imaging (MPI) has emerged as a novel technology utilizing superparamagnetic nanoparticles as tracers, essential for disease diagnosis and treatment guidance in preclinical animal models. Unlike other modalities, MPI provides high sensitivity, deep tissue penetration, and no signal attenuation. However, existing MPI tracers suffer from "always-on" signals, which complicate organ-specific imaging and hinder accuracy. To overcome these challenges, we have developed a responsive MPI tracer using pH-responsive PdFe alloy particles coated with a gatekeeper polymer. This tracer exhibits pH-sensitive Fe release and modulation of the MPI signal, enabling selective imaging with a higher signal-to-noise ratio and intratumoral pH quantification. Notably, this responsive tracer facilitates subtraction-enhanced MPI imaging, effectively eliminating interference from liver uptake and expanding the scope of abdominal imaging. Additionally, the tracer employs a dual-function mechanism for adaptive cancer therapy, combining pH-switchable enzyme-like catalysis with dual-key co-activation of ROS generation, and Pd skeleton that scavenges free radicals to minimize Fe-related toxicity. This advancement promises to significantly expand MPI's applicability in diagnostics and therapeutic monitoring, marking a leap forward in imaging technology.

Effect of colloidal magnetite (Fe3O4) nanoparticles on the electrical characteristics of the azolectin bilayer in a static inhomogeneous magnetic field

This work is devoted to the study of the combined effects of applied magnetic field and MNPs on the electrical characteristics of bilayer lipid membranes. We present results of the study of electrical parameters of azolectin membranes in a static inhomogeneous magnetic field at the one-sided addition of positively charged quasi-spherical superparamagnetic magnetite nanoparticles with a diameter of about 4 nm. The magnet was located at different distances from the membrane, and the magnetic field attracted the nanoparticles to the membrane surface with different strengths. We observed three pronounced effects that depended on the external magnetic field. Firstly, after addition of nanoparticles in a magnetic field, the conductance of the membranes increased. A smooth increase in conductance was accompanied in some cases by the appearance of current jumps, which can be associated with the formation of through pores with a radius of no more than 1 nm. The conductance increased with increasing magnetic field gradient. Secondly, at zero command voltage, a negative current through the membrane was observed. Although our experiments did not allow us to unambiguously determine which particles create this current, we believe that this current is associated with the penetration of particles through the membrane. This effect intensified with increasing magnetic field gradient. Thirdly, we observed a sharp change in the nonlinear dependence of capacitance on voltage associated both with the change in the surface potential of the azolectin membrane and with the effect of MNP binding to the membrane surface on the apparent membrane capacitance.

In vitro exposure of porcine sperm to functionalized superparamagnetic nanoparticles.

Nanotechnology and its applications have advanced significantly in recent decades, contributing to various fields, including reproduction. This study introduces a novel method to label porcine oocytes with nanoparticles (NPs) bound to oviductin (OVGP1, Ov) for use in Assisted Reproductive Technologies (ARTs). Despite promising developments, concerns about NP toxicity in gametes necessitate thorough investigation. This research aims to assess the impact of functionalized NPs (NPOv) on sperm functionality. Boar sperm were co-incubated with NPOv for 0, 0.5 and 1 h in two media: BTS (semen dilution and conservation) and TALP (sperm capacitation and invitro fertilization-IVF). Sperm quality parameters (viability, motility and kinematics) showed no significant differences in TALP medium (p > .05). In BTS, althoughsome kinetic parameters were altered, motility, progressive motility and viability remained unaffected (p > .05). Additionally, NPs presence on the zona pellucida (ZP) of oocytes did not affect sperm attachment (p > .05). In conclusion, invitro exposure of boar sperm to OVGP1-functionalized NPs in IVF medium or attached to the ZP surface of matured oocytes does not impair sperm functionality, including their binding ability to the ZP.

Phosphonated Polyetheramine-Coated Superparamagnetic Iron Oxide Nanoparticles: Study on the Harsh Scale Inhibition Performance of Calcium Carbonate and Barium Sulfate.

Harsh scale buildup, such as calcium carbonate (calcite) and barium sulfate (barite), poses significant challenges in the oil and gas industry. While various scale inhibitors (SIs) are employed to mitigate this issue, there is a need for greener, more efficient, compatible, and affordable alternatives. Calcium compatibility often complicates the use of SIs, potentially leading to formation damage. Our group has recently synthesized a recyclable nanocomposite scale inhibitor made of superparamagnetic nanoparticles coated with a trisodium citrate linker to phosphonated poly(ether amine) (SPION-TSC-PPEA) and tested it against calcium sulfate (gypsum), a simple form of scale buildup. This study evaluates the recyclable nanocomposite scale inhibitor's efficiency in mitigating calcite and Barite scales through static jar tests and high-pressure dynamic tube-blocking tests at 80 bar and 100 °C. The nanocomposite demonstrated high calcium ion compatibility, excellent inhibition efficiency against calcite, moderate efficiency against Barite, and maintained efficacy over five recycling cycles.

Development and characterization of superparamagnetic Zn-Doped Nickel ferrite nanoparticles

The nanocrystalline Zn-doped Nickel ferrite nanoparticles, ZnxNi1-xFe2O4 (x = 0.2, 0.4, 0.6 and 0.8) have been developed using the sol–gel method. The average crystallite size was calculated using the Debye- Scherrer formula through X-ray diffractometry and found to be in the range of ∼ 4 nm to 7 nm. Rietveld refinement of the prepared samples suggested the cubic spinel phase of the nanoparticles. For the surface morphology analysis of the nanoparticles, the Field Emission Scanning Electron Microscope (FESEM) technique was utilized, which showed the spherical shape of the particles. Elemental Dispersive X-ray spectroscopy (EDAX) ascertained the experimentally obtained elemental composition with the calculated elemental composition and identified the elements present within the sample. Fourier Transform Infrared Spectroscopy (FTIR) revealed the two prominent absorbing bands, i.e., tetrahedral complex, υ1 and octahedral complex, υ2 in the range of 4000–300 cm−1. Photoluminescence spectroscopy and UV Visible diffuse reflectance spectroscopy determined the optical properties of the samples and it was observed that with the increasing concentration of Zinc, the value of band gap decreased from 2.29 eV to 2.11 eV. The M−H curves of the Zn-doped nickel ferrites exhibit superparamagnetic behavior at room temperature, confirming the presence of a single domain in the sample.

Enhanced functionalization of superparamagnetic Fe3O4 nanoparticles for advanced drug enrichment and separation applications

Backgroundsuperparamagnetic ferroferric oxide (Fe3O4) nanoparticles can be extensively functionalized for applications in drug enrichment and separation. Their high magnetic responsiveness and controllable surface modification enable rapid drug enrichment and separation under external magnetic fields. This study aimed to enhance the application potential of superparamagnetic Fe3O4 nanoparticles in the field of drug enrichment and separation by functionalizing these nanoparticles to improve their biocompatibility and targeting capabilities.Methodssuperparamagnetic Fe3O4 nanoparticles functionalized with dopamine were synthesized using benzyl alcohol as the solvent and iron acetylacetonate as the precursor. The dopamine-functionalized superparamagnetic iron oxide nanoparticles were used to analyze protein enrichment and separation. Characterization of the nanoparticles was conducted, including analysis of particle size distribution, Zeta potential, and fluorescence spectra using a fluorescence spectrophotometer.Resultsthe Fe3O4 nanoparticles maintained high magnetism from the original material and exhibited uniform particle size distribution and stable Zeta potential. The saturation magnetization of dopamine-functionalized superparamagnetic Fe3O4 nanoparticles showed no significant difference compared to before coating, indicating minimal influence of dopamine on the internal magnetic core of the nanoparticles. The Fe3O4 nanoparticles demonstrated good biocompatibility and stability.Conclusionfunctionalization of superparamagnetic Fe3O4 nanoparticles significantly enhances their efficiency in drug enrichment and separation processes, suggesting broad applications in the pharmaceutical industry.

A FAPα-activated MRI nanoprobe for precise grading diagnosis of clinical liver fibrosis

Molecular imaging holds the potential for noninvasive and accurate grading of liver fibrosis. It is limited by the lack of biomarkers that strongly correlate with liver fibrosis grade. Here, we discover the grading potential of fibroblast activation protein alpha (FAPα) for liver fibrosis through transcriptional analysis and biological assays on clinical liver samples. The protein and mRNA expression of FAPα are linearly correlated with fibrosis grade (R2 = 0.89 and 0.91, respectively). A FAPα-responsive MRI molecular nanoprobe is prepared for quantitatively grading liver fibrosis. The nanoprobe is composed of superparamagnetic amorphous iron nanoparticles (AFeNPs) and paramagnetic gadoteric acid (Gd-DOTA) connected by FAPα-responsive peptide chains (ASGPAGPA). As liver fibrosis worsens, the increased FAPα cut off more ASGPAGPA, restoring a higher T1-MRI signal of Gd-DOTA. Otherwise, the signal remains quenched due to the distance-dependent magnetic resonance tuning (MRET) effect between AFeNPs and Gd-DOTA. The nanoprobe identifies F1, F2, F3, and F4 fibrosis, with area under the curve of 99.8%, 66.7%, 70.4%, and 96.3% in patients’ samples, respectively. This strategy exhibits potential in utilizing molecular imaging for the early detection and grading of liver fibrosis in the clinic.

A DNA machine-based magnetic resonance imaging nanoprobe for in vivo microRNA detection

In situ monitoring microRNA (miRNA) expression in vivo holds immense potential for directly visualizing the occurrence and progression of tumors. However, the significant barrier to developing a probe that can overcome the low abundance of miRNAs while providing an output signal with unlimited tissue penetration depth remains formidable. In this study, we developed a DNA machine-based magnetic resonance imaging nanoprobe (MRINP) for amplified detection of miR-21 in vivo. The MRINP was constructed with superparamagnetic Fe3O4 nanoparticles (NPs), paramagnetic Gd-DOTA complexes, and miR-21-activated DNA machines; the DNA machine was composed of hairpin DNAzyme (HD) strands serving as the DNAzyme walker and hairpin substrate (HS) strands serving as the track. Once uptake into tumor cells, the intracellular miR-21 specifically recognized and hybridized with the HD strand, restoring the activity of DNAzyme. Subsequently, the DNAzyme walker autonomously traveled on the surface of MRINP, and each step movement of the DNAzyme walker resulted in the cleavage of its substrate strands and the ensued release of the Gd-DOTA complex-labeled oligonucleotides, turning on the T1 signal of Gd-DOTA complexes for in situ imaging of miR-21 in tumor-bearing mice. This strategy would offer a promising approach for mapping tumor-specific biomarkers in vivo with unlimited penetration depth.

A review of superparamagnetic nanoparticles applications and regulatory aspects in medicine and environmental areas

A review of superparamagnetic nanoparticles applications and regulatory aspects in medicine and environmental areas

Magnetic Monopole-Like Behavior in Superparamagnetic Nanoparticle Coated With Chiral Molecules.

Superparamagnetic iron oxide nanoparticles (SPIONs) have attracted wide attention due to their promising applications in biomedicine, chemical catalysis, and magnetic memory devices. In this work, the force is measured between a single SPION coated with chiral molecules and a ferromagnetic substrate by atomic force microscopy (AFM), with the substrate magnetized either toward or away from the approaching AFM tip. The force between the coated SPION and the magnetic substrate depends on the handedness of the molecules adsorbed on the SPION and on the direction of the magnetization of the substrate. By inserting nm-scale spacing layers between the coated SPION and the magnetic substrate it is shown that the SPION has a short-range magnetic monopole-like magnetic field. A theoretical framework for the nature of this field is provided.

Nanomedicine: Pioneering a New Frontier in Neuro-Ophthalmology

Nanotechnology is one of the most promising fields of study, and it represents a pioneering leap in science and technology by the precise control over materials at the atomic and molecular level. This transformation affects numerous aspects of modern human life, including medicine, healthcare, electronics, computing, and energy storage. Nanotechnology has shown significant advancements in managing various health problems through different nano-formulations. These engineered nano-systems can be used as drug delivery vehicles, gene therapy vectors, imaging agents, etc. A range of neuro-visual disorders have been identified through the years and found to be associated with malfunctioning the eyes and the nervous system. State-of-the-art nano-formulations are currently being examined for their possible beneficial effects in diagnosing and treating various nervous-related ocular conditions. Nano-emulsions and polymeric hydrogels are efficient drug delivery vehicles of anti-glaucoma drugs. Superparamagnetic nanoparticles (NPs) are extensively being used as magnetic tags for the non-invasive imaging of transplanted cells in patients with optic neuritis and bio-engineered sensors are utilized in neuromyelitis optica diagnosis, though the colorimetric detection of anti-aquaporin-4 antibodies by silver NPs. These are just a few of the most recent advancements in neuro-ophthalmology. This review summarizes the central neuro-ophthalmologic disorders affecting the global healthcare system, emphasizing the utilization of revolutionized nanomedicine-based tools for managing these conditions. Addressing the potential challenges and side effects is critical for the safe and effective integration of nanotechnology in various fields of study, especially in healthcare.