Summer Pasture Research Articles

Associations of different genotypes with the meat productivity of sheep

Identification of the potential to increasthe agricultural production of farm animals has become possible through the use of genomic selection methods. It is advisable to improve breeding and pedigree qualities of local breeds, since these have the best adaptability to the features of the natural and geographical conditions in a given area. He controlled slaughter of animals with different genotypes allowed us to determine their meat qualities, such as biological value, slaughter yield and slaughter weight. The ratio of slaughter weight and pre‐slaughter weight gives the slaughter yield in percent.PCR (polyamino acid chain reaction) using specific synthesised sets of oligonucleotides in the genotyping of experimental sheep populations was carried out in order to study the allelic variants of the CAST and GH genes.When studying the indices characterising body weight in sheep which have different genotypes, significant increases in the indices could be observed before the periods of driving to summer pastures (17.2 kg) and during lambing (25.8 kg). The lowest indices were noted during driving both from winter pastures to summer pastures and from summer pastures to winter pastures (30.5 kg).In the period before driving, the live weight index was higher in the CASTMM genotype by 5.8 %; upon arrival in the mountains the difference was 6.1 %; during lambing — 4.7 %; before leaving for winter pastures — 8.4 % and upon arrival at winter pastures — 9.5 %.

PSIX-26 Effectiveness of mineral supplementation during range grazing on mineral status in ewes

Abstract Mineral supplementation in the intermountain west region of the US may be necessary for maintenance of healthy flocks since important trace minerals are deficient in this region. However, rough terrain can make mineral supplementation challenging. The objective of this study was to evaluate the trace mineral status of ewes on range. We hypothesized that ewes with access to a mineral supplement would have decreased risk of mineral deficiency. In this study, ewes (n = 59) grazed four summer pastures on Cedar Mountain near Cedar City, Utah. Ewes between the ages of 1 and 6 yr of age were stratified by initial body weight (BW) = 87 kg ± 13 kg and breed [black face (BLF; Suffolk) and white face breeds (WF; Targhee, Rambouillet, and their crosses)] into two treatment groups 1) trace mineral supplement ad libitum (TRT; n = 7 BLF and 23 WF) or 2) no mineral supplement (CON; n = 15 BLF and 14 WF). All ewes received a diet of mixed alfalfa and triticale hay without mineral supplementation 30 d before the trial began. A SmartFeed (C-lock Inc.) was used to track individual animal mineral intake. Plasma samples were collected via jugular venipuncture at d 0, 34, 77, and 116. A clinical veterinarian from Utah State University performed liver biopsies at d 0 and 116 on a subset of ewes (n = 10). After the grazing season, plasma and liver samples were sent to the Iowa State University Veterinary Diagnostic Laboratory for analysis of Cu, Mn, Se, and Zn. The mixed procedure of SAS was used to analyze plasma mineral content with day as a repeated measure and individual ID as a random variable. The Glimmix procedure of SAS was used to analyze liver mineral content. Plasma Cu and Se increased (P ≤ 0.05) by day in all ewes throughout the grazing season. No differences related to treatment or breed (P > 0.10) were observed for plasma Cu or Se concentrations. Plasma Mn and Zn decreased (P < 0.0001) by 1.5 ppb and 0.4 ppm, respectively, between d 0 and 116 with no treatment effect (P > 0.10). BLF ewes had 0.3 ppm (P = 0.01) greater plasma Mn concentrations than WF ewes, with no difference (P = 0.6) observed due to treatment. Hepatic Cu and Se increased (P ≤ 0.05) by 114 ppm and 1 ppm, respectively, between d 0 and 116. Hepatic Mn concentrations did not differ (P > 0.10) by day or treatment. Hepatic Zn concentrations tended to increase by day (P = 0.07), 23 ppm between d 0 and 116. CON ewes had 28 ppm greater (P = 0.04) hepatic Zn concentrations than TRT ewes. Additional research is warranted to understand why CON had greater hepatic Zn concentrations than TRT, which may indicate that diverse rangeland forages may provide sufficient concentrations for sheep production in summer months.

Detection and Genetic Characterization of Border Disease Virus (BDV) Isolated from a Persistently Infected Sheep in a Migratory Flock from Rajasthan State, Northwestern India.

Border disease virus (BDV) causes significant economic losses in sheep farming worldwide. In India, BDV has not yet been studied in sheep migrating for summer pasturing. This study aimed to determine the extent of BDV infection in migratory sheep and provide genetic characteristics of BDV. Blood and serum samples from 90 lambs of a migratory sheep flock (600) in Central India were collected and subjected to molecular detection, phylogenetic analysis and virus neutralization test (VNT). We detected BDV in two lambs through real-time RT-PCR, while 64.4% (58/90) of in-contact lambs had BDV neutralizing antibodies. One apparently healthy lamb was found to be persistently infected with BDV. Phylogenetic analysis of 5'-UTR and Npro genes and the concatenated datasets typed the BDV isolate from PI sheep as BDV-3 genotype. However, it showed a closer relationship with BDV-3 strains from China than the previously reported Indian BDV-3 strains. This is the first report on the detection of BDV persistently infected migratory sheep in India. Additionally, we provided evidence of genetic variability among BDV-3 strains in India. The findings improve our understanding of epidemiology and genetic characteristics of BDV in India and highlight the potential risks associated with the traditional practice of sheep migration for summer pasturing.

Bioecological Characterization of Nematode Distribution Among Livestock in the North-Eastern Territories of the Lesser Caucasus of Azerbaijan Republic

Helminthological studies of sheep and goats were conducted in the northeastern territories of the Lesser Caucasus of the Republic of Azerbaijan in 2013-2022. The northeastern areas of the Lesser Caucasus include Ganja and Goygeli, Dashkesan, Geranboy, Naftalan, Samukh, Shamkir, Tovuz, Agstafa, Gazakh and Kadabek districts, as well as the areas around the Kura and Jeyranchel rivers, which are wintering places of livestock farms. This process plays a special role in the formation of helminth fauna of animals, as small horned livestock farms in these areas lead a nomadic and sedentary lifestyle. As a result of helminthological research 52 species of helminths, including 43 species of nematodes were identified. Of these, 7 species are indicated for the study region for the first time. All detected nematode species were registered in sheep, while only 26 species were found in goats, which are common to the sheep helminth fauna. These helminth species can be considered as permanent elements of the helminthofaunistic complex of livestock production in the studied region. It was found that young sheep and goats are relatively heavily infested with helminths. Infestation is higher in late spring — early summer and early fall. For this purpose, 957 sheep and 619 goats were examined for nematode pathogens in different landscape-ecological zones of the region by the method of complete helminthological autopsy according to K. I. Skryabin. Some species of helminths are absent in the studied animals before driving them to summer pastures but are observed in them after their return to wintering. Animals become infected by ingesting larvae or mature helminth eggs on summer pastures. -

Ecological problems of the plant cover of the northeastern slope of Great Caucasus (within Azerbaijan)

Highlight the purpose. Plant cover of our republic, especially forest ecosystems, has lost its original state as a result of the influence of natural and anthropogenic factors. The study of the impact of these factors on biodiversity has been the focus of many experts. The settlement of the studied area since ancient times and the good development of agriculture have caused the degradation and erosion of the forest cover that covers large areas, as well as the vegetation that replaces each other in the altitude zones here. For this reason, the issues of protection and protection of vegetation are distinguished by their relevance. The main goal of our research is to protect the vegetation of forests that have changed their initial state. Objectives. Mountain geosystems of the northeastern slope of the Greater Caucasus are bordered by the Russian Federation to the north, northwest, and west, the Samur-Devachi plain to the east, and the watershed line of the Greater Caucasus Range and Atachay to the south and southeast. Methods. Field studies were organized to solve the issues raised during the research period. Short-term visual observations of the existing geoecological conditions were carried out, from topographic maps and other maps (landscape, vegetation, etc.) of various scales, as well as from 1:25000 scale aerial and space images in the fund of the Institute of Ecology of MAKA, “Google” and “Bing” space images with a resolution of 1 meter in the funds, taken in the visible zone of the spectrum, obtained from various satellites in 2020-2023 and based on the application of SASPlanet, ArcGIS processing programs were used. With the help of these materials, the vegetation degradation and erosion processes of the research area were studied. Results. The northeast part of the Great Caucasus differs from the other mountainous regions for its appropriation features. For a long time appropriation of the investigated zone by the various farming areas caused a serious change in the natural geosystems. Thus, the zones where a hypsometric height of 200-500 m are mainly replaced by agro-complexes, the parts within 500-1800 m turned into a life and rest objects together with the arable and pastures, an irregular use from the summer pastures in cattle-breeding in the zones higher than 1800 m was completed by exposing of the plant cover to transformation. During assimilation, activation of ecological problems like decreased plant productivity, intensification of the erosion process, acceleration of degradation, and so on activates a problem of rational use. Defining importance, sensitiveness of plant cover, and rational use ways as protection, refusal of usage, extensive use of protection, development, extensive use, improvement, and development were determined.

Effects of Nitrogen Sources on Primary and Secondary Production from Annual Temperate and Tropical Pastures in Southern Brazil

Improvements in nitrogen use efficiency can be achieved through fertilizer management strategies that capitalize on nutrient synergies. However, limited research on synergies between nitrogen, sulfur, and calcium complicates understanding causal links and developing sustainable management. In this regard, the effects of different nitrogen sources on productivity and nitrogen use efficiency in Italian ryegrass (Lolium multiflorum Lam.) and pearl millet (Pennisetum glaucum (L.)), along with their impacts on forage quality and secondary production, were investigated. Treatments included: Urea (46% N), ammonium nitrate (NH4NO3; 32% N), ammonium nitrate supplemented with calcium and sulfur (NH4NO3 (+), 27% N + 5% Ca + 3.7% S), and control treatment with no N application. The application of fertilizers that combine nitrogen with calcium and sulfur enhances primary production in both winter and summer pastures. Fertilization with NH4NO3 (+) increased nitrogen use efficiency by 125% in Italian ryegrass compared to NH4NO3. However, within the framework of rotatinuous grazing management principles, optimizing plant nitrogen use efficiency does not necessarily lead to a better forage quality or animal performance. These findings highlight that using fertilizers that promote synergies among nutrients, such as the combination of nitrogen with calcium and sulfur, can bring benefits to the sustainability of pasture-based livestock production systems.

Energy supplementation of beef steers or inclusion of legumes in temperate pastures in crop-livestock integration area

The most common way of using the crop-livestock integration system in subtropical regions is cultivating soybeans or corn during the summer and temperate pastures in the winter. The objective of this study was to evaluate different beef cattle finishing systems in an area of crop-livestock integration. The inclusion of legumes and supplementation on black oat (Avena strigosa Schreb) and ryegrass pastures (Lolium multiflorum L.) were evaluated. Data from three years of research (2017, 2018 and 2020) were evaluated. Thus, 54 steers (18 per year) were used, with initial age of 22 ± 3 months and 413.08 ± 4.56 kg of initial live weight. They were randomly divided into nine paddocks of 0.7 hectares. The experimental design was randomized blocks with three replicates (paddock with two animals). Supplementation provided greater carrying capacity (1406.0 vs. 1269.6 kg/ha), average daily weight gain (1.4 vs. 1.1 kg/day), and, consequently, greater gain per area (384.5 vs. 302 kg BW/ha). Animals that received energy supplementation presented higher slaughter weight (536 vs. 510 kg), weight (287.1 and 286.2 vs. 266.2 and 265.3 kg), and hot (53.6 vs. 52.1%) and cold (53.4 vs. 52%) carcass yield, as well as higher fat content in the carcass (265 vs. 234 g/kg). The legume in the pasture did not affect the performance or characteristics of the animal carcasses. The supplementation increased the performance and carcass parameters, but did not influence the qualitative characteristics of the meat. In addition to individual performance, supplementation increased the pasture's carrying capacity, improving the system's productivity.

Land intensification use scenarios based on urban land suitability assessment of the national park

In designing national parks, it is important to balance land use with ecological protection and local socio-economic development. In the past decades, the national park has undergone landscape fragmentation and degradation of ecological functions due to climate change and land use change, especially in national parks with urban areas. Therefore, ecological environmental protection projects should include the assessment of land use when planning urban area development in the national park region. Consequently, we used the Land Suitability Evaluation (LSE) method, with 13 indicators for natural, social and ecological variables, to assess the suitability of the newly created national park for urban land use on the Tibetan Plateau. Combining results of the suitability assessment and the Cellular Automata (CA) method, three urban intensification scenarios were simulated for 2040: (1) unplanned, (2) intensified, and (3) strictly regulated urban growth boundaries (UGBs). The impact of land use on the normalized difference vegetation index (NDVI) was analyzed by remote sensing and GIS tools, and the landscape patterns of the three urban growth scenarios were calculated. Close to 87 % of the area was basically suitable, nearly 13 % was unsuitable, and only 0.06 % (43.4 km2–also included in the basically suitable) was suitable for urban land use. In 2040, the built-up areas are predicted to be 3.38, 2.71 and 2.32 times greater than in 2020 under the unplanned (without considering ecological restrictions), the intensive development (emphasizing the driving force of transportation and tourism on the expansion of built-up areas) and the strict control (maximizing ecological restrictions) scenarios, respectively. An ideal development model was used to balance ecological protection and population growth and economic development. The NDVI analysis revealed that the impact of land use on vegetation cover in the intensive development scenario was ranked as winter pastures > tourist development zones > summer pastures. It is concluded that the intensification scenario, with transport and tourism as the main spatial drivers, is the most desirable for the future coordination of ecological conservation and socio-economic development in the national park. This scenario may alleviate the current environmental pressure of livestock grazing on winter pastures in the national park on the Tibetan Plateau. However, realizing this model requires appropriate engineering design for town development. Future research should assess the impacts of urban growth on the ecological environment, with the aim to achieve sustainable urban development tailored to the various geographic regions.

A Proposed Typology of Farming Systems for Assessing Sustainable Livelihood Development Pathways in the Tien Shan Mountains of Kyrgyzstan

In Kyrgyzstan, most farming systems focus on animal husbandry, which depends on mixtures of crops and pastures around settlements and higher-elevation summer pastures. These farms face the problems of insufficient fodder production and pasture degradation due to overgrazing, resulting in low productivity of livestock and reduced household incomes. The spatial diversity of farms often hampers the development of interventions aimed at improving crop and animal productivity, as well as sustainable grassland management, while the absence of a comprehensive and systematic classification system that effectively encompasses the diverse range of livelihood strategies within farming systems presents a significant obstacle to the advancement of initiatives promoting sustainable livelihoods. This study aimed to develop a consistent typology of smallholder farms in the Tien Shan using multivariate analysis. By analyzing data from 235 farm-households and evaluating key classification variables, we identified two distinct farming systems, upper mountain farms and lower mountain farms, based on socioeconomic and agro-ecological characteristics. Our typology considers elevation, grazing period, cultivated area, and off-farm income and better captures the diversity of farming activities and household income compared to current classification models. These findings will inform and tailor policies and interventions suitable for enhancing sustainable livelihoods in Kyrgyzstan’s mountain farming systems.

Detecting Floral Resource Availability Using Small Unmanned Aircraft Systems

Floral resources for native pollinators that live in wildland settings are diverse and vary across and within growing seasons. Understanding floral resource dynamics and management is becoming increasingly important as honeybee farms seek public land for summer pasture. Small Unmanned Aircraft Systems (sUASs) present a viable approach for accurate broad floristic surveys and present an additional solution to more traditional alternative methods of vegetation assessment. This methodology was designed as a simplified approach using tools frequently available to land managers. The images of three subalpine meadows were captured from a DJI Phantom 4 Pro drone platform three times over the growing season in 2019 in Sanpete County, Utah. The images were composited using Pix4D software 4.5.6 and classified using a simple supervised approach in ENVI 4.8 and ArcGIS Pro 2.4.3 These same meadows were assessed using two traditional ocular methods of vegetation cover–meter-squared quadrats and macroplot estimation. The areas assessed with these methods were compared side by side with their classified counterparts from drone imagery. Classified images were not only found to be highly accurate when detecting overall floral cover and floral color groups (76–100%), but they were also strongly correlated with quadrat estimations, suggesting that these methods used in tandem may be a conducive strategy toward increased accuracy and efficiency when determining floral cover at broad spatial scales.

Sobressemeadura de pastos anuais de verão na cultura da soja para suprir a escassez de foragem outonal

ABSTRACT: In integrated crop-livestock systems, overseeding annual summer pastures over grain crops can reduce forage shortages in autumn without altering grain production. This study evaluated the influence of pearl millet (Pennisetumglaucum L.) and sudangrass (Sorghum Sudanese) overseeded on a soybean (Glycine Max) crop or no-tillage seeding after soybean harvest on productive aspects of pasture. The experimental design was a randomized block with treatments in a 2 × 2 factorial arrangement (pearl millet or sudangrass, overseeding in the full seed stage (R6) of soybean crops or seeding them in a no-tillage system after soybean harvest) in two agricultural years. Pasture height, plant stand, tiller density, and forage production were higher in the first crop year. The overseeding strategy resulted in higher pasture height and foraged production in the first crop year and higher pasture utilization for the next two crop years. Under the no-tillage strategy, the plants stand more elevated in the first crop year, resulting in a higher tiller density in the second crop year. Pasture height, utilization period, and forage production did not differ among the forage species. Pearl millet had a more elevated plant stand, while sudangrass had a higher tiller density. Annual summer pasture overseeding of soybean crops increases the pasture utilization period and forage production in autumn.

Risk factors for chronic perforating skin lesions in the area of the digits in cattle on Swiss alpine pastures

Diseases of the digits often occur in cattle on larger cattle mountain pastures. In the late spring 2020, at the time of the ascent of 1554 cattle to 11 high altitude alpine pastures in the Lower Engadine region, lesions in the area of the digits were clinically assessed and documented. 254 cattle were of non-cantonal and 1300 of local origin (Lower Engadine; postal code CH-75XX). Skin lesions in the area of the digits, identified as digital dermatitis (DD; Mortellaro's disease), were further classified according to the DD scoring system. Nonspecific skin lesions with clinical evidence of granulation tissue formation were termed chronic penetrating skin lesions (CPSL). At the end of the alpine pasturing season, in the early fall (descent of cattle from the alpine pastures), the procedure was repeated, and biopsies were taken from randomly selected cattle with CPSL. Digital dermatitis lesions were found in 34 of 1551 cattle at ascent, but no case of CPSL was found at that time. At descent, 19 of 1529 cattle had DD lesions and 88 cattle had CPSL. The clinical appearance of the CPSL was consistent with chronic skin lesions caused by penetrating skin lacerations. Histologically, the majority of the CPSL were classified as chronic hyperplastic dermatitis with granulation tissue formation. In all CPSL biopsies examined by PCR, Fusobacterium necrophorum and Porphyromonas levii, but neither Dichelobacter nodosus nor the tested Treponema species were detected. Fluorescence in situ hybridization revealed a negative result for Treponema species in all biopsies. In the regression analysis, cattle in the age group of 365 to 730 days had an increased risk for the presence of CPSL compared to the age group of 160 to 365 days (odds ratio (OR) = 4,95; confidence interval (CI) = 1,97-12,43). Holstein cattle had an increased risk of developing CPSL compared to Brown cattle (OR = 2,92; CI = 1,46-5,86) and cattle of non-cantonal origin showed a massively higher risk compared to local cattle (OR = 10,59; CI = 5,79 - 19,37). The statistically significant associations found in the present study can be taken into account in the selection of animals for summer pasturing on high altitudes in the future in order to reduce the prevalence of CPSL and consequently reduce the antimicrobial use. Spread of DD during the alpine pasturing season within the cattle groups examined was not found.

Goat hair as a bioindicator of environmental contaminants and adrenal activation during vertical transhumance.

Autochthonous breeds of livestock are considered a pivotal genetic resource for agriculture, rural development, and food and nutrition security. In the Italian Alps, local livestock breeds are maintained using the traditional alpine farming system based on vertical transhumance, with the use of alpine pastures from late spring to autumn and indoor housing with a hay-based diet for the remaining part of the year. Because of their tight link with the territory of origin, local breeds could be used to biomonitor environmental contaminations. Moreover, animal welfare should also be monitored during transhumance in animals, which are exposed to a sudden farming system change and different types of stressors. For these reasons, this investigation hypothesized that the content of trace elements, heavy metals, and cortisol in the hair of goats changes during vertical transhumance, possibly reflecting different dietary contents and activity of the hypothalamic-pituitary-adrenal (HPA) axis. This study aimed to assess the response of an Italian local goat breed to the change from indoor housing to alpine pasture in summer in terms of hair concentrations of (i) trace elements and heavy metals and (ii) cortisol. The regrown hair of Frisa goats was monthly collected for 2 consecutive years (n = 10 for heavy metals and trace elements and n = 6 for cortisol in 2021, n = 17 for both analyses in 2022), once before vertical transhumance and twice after that event. Hair was then analyzed for trace elements, heavy metals, and cortisol by inductively coupled plasma-optical emission spectrophotometer (ICP-OES) and enzyme immunoassay (EIA), respectively. Data were analyzed by multilevel models. The results showed an increase in As content during alpine pasture (p < 0.01), probably reflecting the soil and water As contents of the grazing area, while Mg, Zn, and Al (p < 0.01) followed the opposite trend, decreasing in the second month after vertical transhumance. Hair cortisol concentrations increased during 2 months of alpine pasture (p < 0.001), indicating an increase in the activation of the HPA axis, in agreement with previous studies. Future investigations can consider a longer study period and the development of ad hoc animal welfare indicators.

Activity budget and movement patterns of Brown Swiss and Alpine Grey lactating cows during summer grazing in alpine pastures

We used GPS tracking to monitor the grazing patterns of Brown Swiss and Alpine grey lactating cows on an alpine summer pasture (2038 m a.s.l.; SD = 146) in the Dolomites. The pasture (171 ha) was managed with a continuous grazing system (0.52 LU/ha) with morning and evening milking in the barn, guided grazing during the ‘day’, and free grazing at ‘night’. GPS positions were collected from 8 Brown Swiss multiparous and 9 Alpine Grey (4 primiparous and 5 multiparous) cows every two minutes. We inferred behaviours (grazing, resting, walking) from movement metrics, activity sensors and direct behavioural observations. After excluding milking periods, the cows grazed for 8 h/d, rested 10–11 h/d, and walked for 1.5/d. Grazing extended into late evening after milking, and resting prevailed throughout the ‘night’ until the morning milking. When grazing and resting, cows mainly used grasslands as the preferred habitat, but forest and sparse shrub were also used remarkably without consistent negative or positive selection. The pasture use was highly heterogeneous, with higher animal loads close to the barn, especially at night, and in areas with gentler slopes. Alpine Grey primiparous cows were less limited by slope and distance from the barn in their movement but were more selective in habitat use than multiparous cows. Differences between multiparous cows of the two breeds were less marked. Further studies should help understand the internal and external drivers of cattle grazing patterns to devise management practices combining animals’ productivity and welfare with the conservation of the grassland ecosystem services.

PSIV-12 Monitoring Ewe Forage Selectivity and Consumption of Tall Larkspur (Delphinium Spp.) in Southern Utah Via Visual Observation and Fecal Metabarcoding

Abstract In Southern Utah, sheep are utilized to a greater extent to graze rangeland due to the presence of Tall Larkspur (Delphinium spp.), which is known to be toxic to cattle. The objectives of this study are to quantify to what degree sheep consume Tall Larkspur and identify forage preferences on a diverse rangeland. We hypothesize that ewes consume tall larkspur based off preferences applied to other forages across range. In this study, ewes (n = 400) were grazed across four summer pastures on Cedar Mountain near Cedar City, Utah. Ewe breed types consisted of Suffolk, Targhee, Rambouillet, and their crosses. During the 116-day trial plant consumption type, time of day, forage availability, weather, range utilization, and apparent weight of sheep were monitored and recorded. Data were collected via a 5-minute bite count procedure, noting all variables above, for each observed ewe. Fecal samples were collected and used for fecal metabarcoding (fDNA) at approximately 30% pasture utilization, which measures the proportion of plant DNA in the feces to determine forage species consumed. Plant species were sorted into functional groups (forbs, grass likes, grasses, shrubs, and trees). Data were evaluated through a CART analysis in R v4.1.2 and stratified using the GLM procedure in SAS v9.4. There was an increase in consumption of forbs, shrubs, and grasses over time, (P &amp;lt; 0.001), (P = 0.01), and (P &amp;lt; 0.001), respectively. For the grass-like and lichen plant types, there is little evidence of forage preference, (P = 0.7) and (P = 1.00) respectively. Fecal metabarcoding analysis indicates that protein content of diet originated from forbs, grass likes, grasses, shrubs, and trees by approximately 57%, 1%, 5%, 6%, and 31%, respectively. Tall Larkspur appeared in fDNA samples, but did not represent a significant portion of the diet. Our findings indicate that sheep in Southern Utah utilize tall larkspur on range to a small extent in their diet, but do not preferentially graze it over other forb species.

216 Alternative Trace Mineral Supplementation Strategies for Beef Cattle

Abstract This study evaluated an injectable trace mineral (ITM) source containing Cu, Mn, Se, and Zn (Multimin90) administered to gestating beef cows receiving a free-choice 2:1 mineral supplementation. Two hundred and eight multiparous and primiparous cross-bred cows (BW = 621 kg ± 96.6 kg; avg. 4 yr. old) were randomly allotted into one of two treatments and received either i) ITM + inorganic, granular 2:1 trace mineral free-choice; or ii) inorganic, granular 2:1 trace mineral free-choice alone (CON). A cross-over design was used for the study and cows in year 2 (n = 202) were allocated to the other treatment group. The ITM was administered at 4 weeks pre-calving and 4 weeks pre-breeding. Body weight and body condition score (BCS) were evaluated at injection times and pregnancy diagnosis using a weigh scale and a 5-point BCS scale (Lowman et al., 1976). Mineral status was determined from blood serum mineral concentration at 4 weeks pre-calving, 4 weeks pre-breeding, and at pregnancy diagnosis. Cows grazed grass-legume pastures in the summer and had access to dugout and well water sources. Cows were managed as two groups during spring and while on summer pasture during a 63-day breeding season until weaning. Serum blood mineral concentrations at 4 weeks pre-calving, 4 weeks pre-breeding, and pregnancy diagnosis did not differ (P &amp;gt; 0.05) between treatment groups. Copper conetrations at pregnancy diagnosis tended (P = 0.09) to be greater for the ITM cows compared with CON cows. At 4 weeks pre-calving and pregnancy diagnosis, Cu and Mn concentrations were numerically greater in the ITM group compared with the CON group. There were no significant results (P &amp;gt; 0.05) for cow BW, BCS, and days pregnant. Over 2-yr, pregnancy rates were 93.6% and 88.6% for the ITM and CON cows, respectively. Calves born to ITM and CON cows did not differ (P &amp;gt; 0.05) for birth weight, Julian birth date, age at weaning, average daily gain, or adjusted 205 d wean weight. However, CON calves tended (P = 0.07) to be heavier at weaning. Study findings suggest that administering an ITM source to beef cows does not improve cow and calf performance, which suggests that more research in this area is required. Additionally, pregnancy rates in beef cattle may be improved with the use of an injectable trace mineral.

376 Effects of Mineral Supplementation During Range Grazing on Ovine Performance

Abstract Western US sheep production systems are dependent on rangeland forages to meet nutritional needs for animal production. While many native rangeland soils and forages are deficient in minerals like Cu, Se, and Zn, it is unknown whether ewes will alter forage selection if they receive a mineral supplemented diet and if alternative forages will affect performance on range. The objectives of this study are to quantify the effects of mineral supplementation on ewe forage selectivity on rangeland, range utilization, and animal performance. We hypothesized ewes who receive a trace mineral supplement will have a more diverse diet, greater rangeland utilization, and improved performance characteristics than their non-supplemented contemporaries. In this study, (n = 59) ewes were grazed across four summer pastures on Cedar Mountain near Cedar City, Utah. Ewes between the ages of 1 and 6 with an average weight of 87 Kg ± 13 Kg were stratified by initial weight and breed into one of two different treatment groups: 1) trace mineral supplement ad libitum (TRT; n = 30), or 2) no mineral supplement (CON; n = 29). Ewe breed types consisted of black face type breeds (BLF; Suffolk) and white face type breeds (WF; Targhee, Rambouillet, and their crosses). All ewes received a similar diet of alfalfa hay and triticale without access to ad libitum mineral supplementation 30 days before the start of the trial. A solar powered C-lock inc. pro was used to track individual animal mineral intake. Back fat (BF) and loin eye depth (LED) were measured at day 0 and day 116 of the trial via ultrasound. Additionally, fecal samples were collected and used for fecal metabarcoding (fDNA) at approximately 30% pasture utilization, which measures the proportion of plant DNA in the feces to determine forage species consumed. Plant species were sorted into functional groups (forbs, grass likes, grasses, shrubs, and trees). The GLM procedure of SAS was used to analyze BF, LED, and fDNA. The mixed procedure of SAS with day as a repeated measure was used to analyze percentage change in body weight. Control ewes had greater BF by 0.08 cm (P = 0.02). No difference in BF was observed by breed (P = 0.8). Loin eye depth was greater by 0.2 cm (P = 0.01) in BLF ewes than WF ewes. Supplemented ewes maintained 1% more of their initial body weight than CON ewes (P = 0.01). No forage selection differences were observed by treatment or breed (P&amp;gt; 0.2). The current study results indicate that mineral supplementation on range may not affect forage selectivity but can benefit animals by maintaining body weight over the grazing season.

Fertilization can accelerate the pace of soil microbial community response to rest-grazing duration in the three-river source region of China.

Overgrazing leads to grassland degradation and productivity decline. Rest-grazing during the regreen-up period can quickly restore grassland and fertilization is a common restoration strategy. However, the effects of rest-grazing time and fertilization on soil microorganisms are unclear in the alpine grasslands. Therefore, the experiment of rest-grazing time and fertilization was carried out to explore the response of soil microorganisms to rest-grazing time and fertilization measures. A field control experiment with rest-grazing time and fertilization as factors have been conducted from the time when grass returned to green till the livestock moved to the summer pasture in Dawu Town of Maqin County of China. The primary treatment we established was the five rest-grazing time, including rest-grazing time of 20 days, 30 days, 40 days, 50 days, and traditional grazing was used as a check group. At the same time, the secondary treatment was nitrogen addition of 300 kg·hm-2 in each primary treatment. The results showed that the total phospholipid fatty acid (total PLFA), actinomyces (Act), and arbuscular mycorrhizal fungi (AMF) showed an ever-increasing biomass with the increase of rest-grazing time and the highest was at 50 days of rest-grazing, and they were all significantly higher than CK. In addition, soil microbial biomass carbon-nitrogen ratio (MBC/MBN) had great influence on the change of microbial community. Applying nitrogen fertilizer can increase the maximum value of biomass of all PLFA groups and the biomass of all PLFA groups changed in an "inverted V" shape with the increase of rest-grazing time. Besides, instead of MBC/MBN, NO3 --N was positively correlated with the biomass of all PLFA groups, which actively regulated the trend of microbial functions. The longer rest-grazing time is more conducive to the biomass of all PLFA groups. However, applying nitrogen fertilizer could break this pattern, namely, the 30 days rest-grazing would be beneficial to the biomass of all PLFA groups. These findings provide key information that rest-grazing during the regreen-up period is benefiscial to the all PLFA groups and fertilization could change the response of microorganisms to rest-grazing, which provide reference measures for the restoration of degraded alpine meadows.

Tracking free-ranging sheep to evaluate interrelations between selective grazing, movement patterns and the botanical composition of alpine summer pastures in northern Italy

Extensive sheep pasturing in alpine regions has a long tradition and fulfils numerous sociological, economic and ecological functions. The effects of sheep grazing on the floristic composition and vice versa depend on various factors. Knowledge of potential interrelations is crucial to developing adequate management systems to maintain pasture productivity and its unique biodiversity. The aim of the present study was to discuss the potential interrelations between movement and selective grazing behaviour of free-ranging unherded sheep and the botanical composition of high-altitude mountain pastures in northern Italy. General movement patterns were determined by using GPS tracking. The floristic composition of areas roamed by the sheep was analysed by collecting physical data during the summer of 2022. The energy content of ingested herbage biomass was determined based on faecal samples. Ranging between 2296 and 3015 m above sea level (a.s.l.), the average altitude used by the sheep was 2654 m a.s.l. Correlation analyses showed that the sheep used significantly higher altitudes with increasing temperature and sunshine duration and with decreasing air humidity and rainfall. A clear selective grazing behaviour was revealed, namely a preference for species with better nutritional attributes. Poa alpina was the most preferred species, while areas dominated by Nardus stricta were avoided. Furthermore, the sheep showed an uphill migration over the season, possibly caused by the delayed start of grassland growth at higher altitudes. Analyses of faecal samples revealed sufficient energy contents, presumably as a result of the targeted selection of nutritious plant species. Future studies should evaluate the feeding value of herbage on offer in order to validate the current results. The study highlights the opportunity of animal tracking in remote areas and provides indications for selective grazing of sheep under conditions of free choice.

Molecular and Cellular Evaluation of Horses With Summer Pasture Associated Asthma Syndrome

Equine asthma is an airway disease that affects a large number of horses annually leading to considerable economic losses in the horse industry. Despite advances in research in this area, there is still a lack of information on its etiology and molecular characterization in pasture associated asthma. The objective of the current study was to characterize the inflammatory disease of lower airways in horses maintained on pasture through cytologic and immunologic profile during the summer in a tropical environment by analysis of the gene expression of Th1 cytokines (IFN- λ, IL-8), Th2 cytokines (IL-4 and IL-5), and pro-inflammatory cytokines (IL-1, TNF-α) in the bronchoalveolar lavage (BAL) fluid in healthy and asthma horses on pasture. A group 39 of clinically healthy horses maintained on native pasture and supplemented with concentrate was evaluated by BAL analyzed for differential cellular count and assigned into a control and an asthma group. The gene expression of pro-inflammatory cytokines was analyzed in the BAL by reverse time PCR (RT-PCR) (IL-1α (alpha), IL-4, IL-5, IL-8, TNF-α alpha and IFN-λ), using β-actin as housekeeping gene. Higher gene expression of IL-1, IL-4, IL-5, IL-8, IFN-λ in the BAL of asthma horses was found. Current results indicate an increase in Th2, characterizing an allergic inflammatory reaction due to the significant increase in IL-5 in asthmatic horses (10.3 ± 1.13), when compared to the values ​​obtained in normal horses (3.27 ± 0.46). The only down regulated cytokine in the asthma group was TNF-α, suggesting a chronic antigenic reaction.