The impact of climate change on agricultural production is apparent due to declining irrigation water availability vis-à-vis rising drought stress, particularly affecting summer crops. Growing evidence suggests that zinc (Zn) supplementation may serve as a potential drought stress management strategy in agriculture. Field studies were conducted using soybean (Glycine max var. Saba) as a model crop to test whether foliar application of zinc oxide nanoparticles (ZnO-NPs) or conventional Zn fertilizer (ZnSO4) would mitigate drought-related water stress and improve soybean yield. Each fertilizer was foliar applied twice at a two-week interval during the flowering stage. Experiments were concurrently conducted under non-drought conditions (70% field capacity) for comparison. Results showed drought significantly reduced relative water content, chlorophyll-a, and chlorophyll-b in untreated control plants by 35.7%, 47.7%, and 41.4%, respectively, compared to non-drought conditions (p < 0.05). Under drought conditions, ZnO-NPs (200mg Zn/L) led to 33.1% and 20.7% increase in chlorophyll-a and chlorophyll-b levels, respectively, compared to ZnSO4 at 400mg Zn/L. Likewise, catalase, peroxidase and superoxide dismutase activities increased by 62.6%, 39.5% and 28.5%, respectively, with ZnO-NPs (200mg Zn/L) under drought compared to non-drought conditions. Proline was significantly increased under drought but was remarkably suppressed (~ 54% lower) with ZnO-NPs (200mg Zn/L) treatment. More importantly, the highest seed yield was observed with ZnO-NPs (200mg Zn/L) treatment under drought (39% higher than untreated control) and non-drought (79.4% higher than control) conditions. Overall, the findings suggest that ZnO-NPs could promote seed yield in soybean under drought stress via increased antioxidant activities, increased relative water content, decreased stress-related proline content, and increased photosynthetic pigments. It is recommended that foliar application of 200mg Zn/L as ZnO-NPs could serve as an effective drought stress management strategy to improve soybean yield.
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