Sulphoquinovosyldiacylglycerol Research Articles

Marine heat waves alter gene expression of key enzymes of membrane and storage lipids metabolism in Phaeodactylum tricornutum

Across the globe, heat waves are getting more intense and frequent. Diatoms are a major group of microalgae at the base of the marine food webs and an important source of long chain polyunsaturated fatty acids that are transferred through the food web. The present study investigates the possible impacts of temperature increase on lipid classes and expression of genes encoding enzymes related to lipid metabolism in Phaeodactylum tricornutum. The heat wave exposure caused an increase in the relative amounts of plastidial lipids such as the glycolipids monogalactosyldiacylglycerol (MGDG), digalactosyldiacylglycerol (DGDG) and sulphoquinovosyldiacylglycerol (SQDG) in parallel with a decrease in the neutral lipid fraction, which includes triacylglycerols. In agreement, gene expression analyses revealed an up-regulation of a gene encoding one MGDG synthase and down-regulation of a diacylglycerol acyltransferase (DGAT), a key enzyme in triacylglycerol synthesis. Our results show that heat waves not only negatively impact the abundance of unsaturated fatty acids such as eicosapentaenoic acid (20:5n-3, EPA) and hexadecatrienoic acid (16:3n-4) as observed by the decrease in their relative abundance in MGDG and neutral lipids, respectively, but also induce changes in the relative amounts of the diverse membrane lipids as well as the proportion of membrane/storage lipids. The expression study of key genes indicates that some of the aforementioned alterations are regulated at the transcription level whereas others appear to be post-transcriptional. The changes observed in plastidial lipids are related to negative impacts on the photosynthesis.

Photosystem I oligomerization affects lipid composition in Synechocystis sp. PCC 6803

In cyanobacteria, increasing growth temperature decreases lipid unsaturation and the ratio of monomer/trimer photosystem I (PSI) complexes. In the present study we applied Fourier-transform infrared (FTIR) spectroscopy and lipidomic analysis to study the effects of PSI monomer/oligomer ratio on the physical properties and lipid composition of thylakoids. To enhance the presence of monomeric PSI, a Synechocystis sp. PCC6803/ΔpsaL mutant strain (PsaL) was used which, unlike both trimeric and monomeric PSI-containing wild type (WT) cells, contain only the monomeric form. The protein-to-lipid ratio remained unchanged in the mutant but, due to an increase in the lipid disorder in its thylakoids, the gel to liquid-crystalline phase transition temperature (Tm) is lower than in the WT. In thylakoid membranes of the mutant, digalactosyldiacylglycerol (DGDG), the most abundant bilayer-forming lipid is accumulated, whereas those in the WT contain more monogalactosyldiacylglycerol (MGDG), the only non-bilayer-forming lipid in cyanobacteria. In PsaL cells, the unsaturation level of sulphoquinovosyldiacylglycerol (SQDG), a regulatory anionic lipid, has increased. It seems that merely a change in the oligomerization level of a membrane protein complex (PSI), and thus the altered protein-lipid interface, can affect the lipid composition and, in addition, the whole dynamics of the membrane. Singular value decomposition (SVD) analysis has shown that in PsaL thylakoidal protein-lipid interactions are less stable than in the WT, and proteins start losing their native secondary structure at much milder lipid packing perturbations. Conclusions drawn from this system should be generally applicable for protein-lipid interactions in biological membranes.

Particulate sulfur-containing lipids: Production and cycling from the epipelagic to the abyssopelagic zone

There are major gaps in our understanding of the distribution and role of lipids in the open ocean especially with regard to sulfur-containing lipids (S-lipids). Here, we employ a powerful analytical approach based on high resolution Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) to elucidate depth-related S-lipid production and molecular transformations in suspended particulate matter from the Northeast Atlantic Ocean in this depth range. We show that within the open-ocean environment S-lipids contribute up to 4.2% of the particulate organic carbon, and that up to 95% of these compounds have elemental compositions that do not match those found in the Nature Lipidomics Gateway database (termed “novel”). Among the remaining 5% of lipids that match the database, we find that sulphoquinovosyldiacylglycerol (SQDG) are efficiently removed while sinking through the mesopelagic zone. The relative abundance of other assigned lipids (sulphoquinovosylmonoacylglycerol (SQMG), sulfite and sulfate lipids, Vitamin D2 and D3 derivatives, and sphingolipids) did not change substantially with depth. The novel S-lipids, represented by hundreds of distinct elemental compositions (160–300 molecules at any one depth), contribute increasingly to the lipid and particulate organic matter pools with increased depth. Depth-related transformations cause (i) incomplete degradation/transformation of unsaturated S-lipids which leads to the depth-related accumulation of the refractory saturated compounds with reduced molecular weight (average 455 Da) and (ii) formation of highly unsaturated S-lipids (average abyssopelagic molecular double bond equivalents, DBE=7.8) with lower molecular weight (average 567 Da) than surface S-lipids (average 592 Da). A depth-related increase in molecular oxygen content is observed for all novel S-lipids and indicates that oxidation has a significant role in their transformation while (bio)hydrogenation possibly impacts the formation of saturated compounds. The instrumentation approach applied here represents a step change in our comprehension of marine S-lipid diversity and the potential role of these compounds in the oceanic carbon cycle. We describe a very much higher number of compounds than previously reported, albeit at the level of elemental composition and fold-change quantitation with depth, rather than isomeric confirmation and absolute quantitation of individual lipids. We emphasize that saturated S-lipids have the potential to transfer carbon from the upper ocean to depth and hence are significant vectors for carbon sequestration.

Influence of thylakoid membrane lipids on the structure of aggregated light-harvesting complexes of the diatom Thalassiosira pseudonana and the green alga Mantoniella squamata.

The study investigated the effect of the thylakoid membrane lipids monogalactosyldiacylglycerol (MGDG), digalactosyldiacylglycerol (DGDG), sulphoquinovosyldiacylglycerol (SQDG) and phosphatidylglycerol (PG) on the structure of two algal light-harvesting complexes (LHCs). In contrast to higher plants whose thylakoid membranes are characterized by an enrichment of the neutral galactolipids MGDG and DGDG, both the green alga Mantoniella squamata and the centric diatom Thalassiosira pseudonana contain membranes with a high content of the negatively charged lipids SQDG and PG. The algal thylakoids do not show the typical grana-stroma differentiation of higher plants but a regular arrangement. To analyze the effect of the membrane lipids, the fucoxanthin chlorophyll protein (FCP) complex of T. pseudonana and the LHC of M. squamata (MLHC) were prepared by successive cation precipitation using Triton X-100 as detergent. With this method, it is possible to isolate LHCs with a reduced amount of associated lipids in an aggregated state. The results from 77 K fluorescence and photon correlation spectroscopy show that neither the neutral galactolipids nor the negatively charged lipids are able to significantly alter the aggregation state of the FCP or the MLHC. This is in contrast to higher plants where SQDG and PG lead to a strong disaggregation of the LHCII whereas MGDG and DGDG induce the formation of large macroaggregates. The results indicate that LHCs which are integrated into thylakoid membranes with a high amount of negatively charged lipids and a regular arrangement are less sensitive to lipid-induced structural alterations than their counterparts in membranes enriched in neutral lipids with a grana-stroma differentiation.

Wound healing potential of Spirulina platensis extracts on human dermal fibroblast cells.

Blue-green alga (Spirulina platensis) is a well renowned nutri-supplement due to its high nutritional and medicinal properties. The aim of this study was to examine the wound healing efficiency of Spirulina platensis at various solvent extracts using in vitro scratch assay on human dermal fibroblast cells (HDF). Various gradient solvent extracts (50 μg/ml of methanolic, ethanolic and aqueous extracts) from Spirulina platensis were treated on HDF cells to acquire its wound healing properties through scratch assay and in this investigation we have used allantoin, as a positive control to compare efficacy among the phytoextracts. Interestingly, aqueous extract were found to stimulate proliferation and migration of HDF cells at given concentrations and enhanced closure rate of wound area within 24 hours after treatment. Methanolic and ethanolic extracts have shown proliferative effect, however these extracts did not aid in the migration and closure of wound area when compared to aqueous extract. Based on phytochemical profile of the plant extracts analyzed by LC-MS/MS, it was shown that compounds supposedly involved in accelerating wound healing are cinnamic acid, narigenin, kaempferol, temsirolimus, phosphatidylserine isomeric derivatives and sulphoquinovosyl diacylglycerol. Our findings concluded that blue-green algae may pose potential biomedical application to treat various chronic wounds especially in diabetes mellitus patients.

The major lipid changes of some important diet microalgae during the entire growth phase

Abstract In the present study, we investigated the major lipid changes of six important diet microalgae (Chlorella sp., Nannochloropsis oculata, Nannochloropsis sp., Isochrysis galbana, Phaeodactylum tricornutum Bohlin and Chaetoceros calcitrans) during the entire growth phase using UPLC-Q-TOF MS system. Our results suggested that EPA/AA-rich lipids were largely detected in both Nannochloropsis sp. and N. oculata as a component of diacylglyceryl-N,N,N-trimethylhomoserine (DGTS). Moreover, docosahexaenoic acids (DHAs) were detected only in I. galbana mainly as a component of monogalactosyldiacylglycerol (MGDG) and sulphoquinovosyldiacylglycerol (SQDG). Besides, the growth phase of microalgae could be a useful variable for the content enrichment of EFA-rich lipids. A higher content EPA/AA-rich DGTS could be obtained at the end of stationary phase for Nannochloropsis sp. and N. oculata, and a higher content of DHA-rich lipids could be obtained at the end of stationary phase for I. galbana. However, EPA/AA-rich lipids were mainly detected in both P. tricornutum and C. calcitrans mainly as a component of TAG and MGDG, respectively. In addition, a higher content of EPA-rich TAG could be obtained at the end of the stationary phase for P. tricornutum. A higher content of EPA-rich MGDG could be obtained at the end of stationary phase for C. calcitrans. Taken together, considering the high EFA requirement for artificial rearing of marine organisms, Nannochloropsis sp., N. oculata, P. tricornutum, C. calcitrans and I. galbana would be highly recommended to be harvested at the end of the stationary phase. The distribution characteristics of EFA-rich lipids related to 6 microalgae are described in detail.

Lead toxicity, defense strategies and associated indicative biomarkers in Talinum triangulare grown hydroponically

Talinum species have been used to investigate a variety of environmental problems for e.g. determination of metal pollution index and total petroleum hydrocarbons in roadside soils, stabilization and reclamation of heavy metals (HMs) in dump sites, removal of HMs from storm water-runoff and green roof leachates. Species of Talinum are popular leaf vegetables having nutrient antinutrient properties. In this study, Talinum triangulare (Jacq.) Willd (Ceylon spinach) grown hydroponically were exposed to different concentrations of lead (Pb) (0, 0.25, 0.5, 0.75, 1.0 and 1.25mM) to investigate the biomarkers of toxicity and tolerance mechanisms. Relative water content, cell death, photosynthetic pigments, sulphoquinovosyldiacylglycerol (SQDG), anthocyanins, α-tocopherol, malondialdehyde (MDA), reactive oxygen species (ROS) glutathione (GSH and GSSG) and elemental analysis have been investigated. The results showed that Pb in roots and shoots gradually increased as the function of Pb exposure; however Pb concentration in leaves was below detectable level. Chlorophylls and SQDG contents increased at 0.25mM of Pb treatment in comparison to control at all treated durations, thereafter decreased. Levels of carotenoid, anthocyanins, α-tocopherol, and lipid peroxidation increased in Pb treated plants compared to control. Water content, cells death and elemental analysis suggested the damage of transport system interfering with nutrient transport causing cell death. The present study also explained that Pb imposed indirect oxidative stress in leaves is characterized by decreases in GSH/GSSG ratio with increased doses of Pb treatment. Lead-induced oxidative stress was alleviated by carotenoids, anthocyanins, α-tocopherol and glutathione suggesting that these defense responses as potential biomarkers for detecting Pb toxicity.

THE LIPID-PIGMENT COMPLEX COMPONENTS OF WINTER WHEAT LEAVES AT INOCULATION WITH MICROORGANISMS POSESSING PHOSPHATMOBILIZATION ABILITY

The evaluation of the physiological condition of winter wheat plants inoculated with phosphate mobilizing microorganisms under the phosphorus deficiency conditions was performed. The influence of PaenibacilluspolymyxaKB,Rhizobiumradiobacter1333andRhizobium radiobacter 5006 on lipid-pigment complexes of plants was observed. The accumulation of the major photosynthetic pigments, increasing of chlorophyll (a+b)/carotenoids ratio and maintaining of high level of phosphatidyl glycerol and stable content of sulphoquinovosyl diacylglycerol was revealed. The inoculation of seeds with phosphate mobilizing microorganisms had significantly improved the productivity of wheat plants in all variants with its highest indices at seeds bacterization with Rhizobium radiobacter 5006.

A sulphonoglycolipid from the fern Polypodium decumanum and its effect on the platelet activating-factor receptor in human neutrophils.

The South American fern Polypodium decumanum, traditional name calaguala, has documented clinical use in oral treatment of skin disorders, including psoriasis. The inflammatory mediator platelet-activating factor (PAF), has been implicated in the pathogenesis of psoriasis. A constituent of a calaguala extract has been shown to have inhibitory activity in a PAF-induced exocytosis model in human neutrophils. The compound was identified as the sulphoquinovosyl diacylglycerol 1,2-di-O-palmitoyl-3-O-(6-sulpho-alpha-D-quinovopyranosyl)-glycero l by spectroscopic means. When subsequently studied in an in-vitro model for [3H]PAF binding in neutrophils from man the compound caused dose-dependent displacement of [3H]PAF from its receptor with an IC50 value of 2 microM. It is suggested that the compound acts through PAF receptor antagonism in intact human neutrophils.

Реакція відповіді рослин на УФ-В опромінення та оксидний стрес

The effect of stress factors – ultraviolet radiation (UV-B) and hydrogen peroxide (H2O2) upon two species of Deschampsia plants – D. antarctica and D. caespitosa were studied. Investigations performed with D. antarctica plant samples delivered of Antarctic and D. caespitosa from Carpathian Mountains showed that UV-B radiation caused changes mainly pigment composition - chlorophyll a and almost all (except violaxanthin) carotenoids. Lipid composition was characterized by accumulation of triacylglicerols, sulphoquinovosyl diacylglycerol and phopsphatydylcholine while monogalacto-syldiacylglycerol quantity decreased. H2O2 treatment cause increase chlorophyll a content in both species and carotenoids in D. antarctica plants. Concerning glycolipid composition one could see monogalactosyldiacylglycerol content decrease in D. caespitosa whereas only insignificant SQDG enlargement was noted in D. antarctica leaves.

Altered Fatty Acid Profile of Polar Lipids in Maize Seedlings in Response to Excess Copper

AbstractA wide range of cellular responses occur when plants are exposed to heavy metal stress, including changes in the membrane fatty acid composition. In this study, we examined the effect of excess copper (100 μm) on the degree of unsaturation [double bond index (DBI) of 18‐C fatty acids] and the polar lipid profiles in maize (Zea mays L.). The level of unsaturation of total fatty acids decreased from 1.42 to 1.28 in maize roots. However, copper‐induced compositional changes of polar fatty acid composition show a general trend of increasing DBI. These responses suggest that individual 18‐C fatty acid classes may have specific roles in maintaining optimal membrane function, enabling plant growth under copper stress. The results also reveal key changes including marked decrease in total root phospholipid levels and consistent increase in the steryl lipid/phospholipid (SL/PL) ratio, as well as a decrease in monogalactosyldiacylglycerol/digalactosyldiacylglycerol (MGDG/DGDG) ratio, reflecting alteration of membrane permeability and fluidity. In shoots, the DBI obtained from phosphatidylcholine (PC), sulphoquinovosyldiacylglycerol (SQDG) and monogalactosyldiacylglycerol (MGDG) was increased. In contrast, these indices were decreased in phosphatidylinositol (PI) and phosphatidylglycerol (PG). However, the PI, PC, PG, MGDG and DGDG contents in shoots were significantly decreased, suggesting alterations in the photosynthetic membranes.

Selective in vivo anti-inflammatory action of the galactolipid monogalactosyldiacylglycerol

The thermophilic blue-green alga ETS-05 colonises the therapeutic thermal muds of Abano and Montegrotto, Italy. Following the isolation, purification and identification of monogalactosyldiacylglycerol (MGDG), digalactosyldiacylglycerol (DGDG), sulphoquinovosyldiacylglycerol (SQDG) and phosphatidylglycerol from ETS-05, we here examine their in vivo anti-inflammatory activities. MGDG, DGDG and SQDG inhibit croton-oil-induced ear oedema in the mouse in a dose-dependent manner. Inhibition by MGDG is greater than that of the reference drug, betamethasone 17,21-dipropionate, and is largely abrogated following acyl group saturation. SQDG is the least potent of these glycoglycerolipids, and shows an early transient effect. In the in vivo carrageenan-induced paw oedema model in the mouse, the inhibitory effects are again dose dependent, with an enhanced efficacy of MGDG over DGDG, SQDG and the reference drug, indomethacin. These compounds are all less toxic than indomethacin. The selective and enhanced inhibitory effects of MGDG over DGDG indicate the mechanisms behind these in vivo anti-inflammatory actions.

Effects of a sub-lethal copper exposure on non-green Acer pseudoplatanus cell lipids

Non-green sycamore cells ( Acer pseudoplatanus L.) treated with 50 μM Cu 2+ showed a reversible arrest of cell growth after 5 d, indicating that the concentration used was sub-lethal for the cells. Growth arrest was accompanied by hexose and nucleotide-phosphate accumulation and, in contrast with previous reports, no polar-lipid degradation was found. Sub-lethal Cu 2+ exposure resulted in an increase in phospholipid amounts, mostly due to an increase of phosphatidylethanolamine. Although the total amount of glycolipids was not significantly changed, Cu 2+ exposure changed the relative concentrations of monogalactosyldiacylglycerol (MGDG), digalactosyldiacylglycerol (DGDG) and sulphoquinovosyldiacylglycerol (SQDG). There was a marked increase of DGDG whereas MGDG and SQDG declined. Our results suggest an increase of DGDG synthesis through a pathway similar to the recently described DGD2 pathway and an inhibition of SQDG synthesis. Furthermore, we observed modifications of fatty acid composition of the plastid synthesised lipids. A significant increase in linoleate (18:2) and a decrease in linolenate (18:3) were found in MGDG, DGDG, SQDG and phosphatidylglycerol (PG), reflecting a deficient desaturation of plastid-located 18:2 fatty acids. In SQDG and PG, Cu 2+ treatment also induced a strong enrichment of palmitate (16:0). The effects of Cu 2+ on DGDG synthesis and fatty acid desaturation are discussed.

A sulphoquinovosyl diacylglycerol is a DNA polymerase epsilon inhibitor.

Sulphoquinovosyl diacylglycerol (SQDG) was reported as a selective inhibitor of eukaryotic DNA polymerases alpha and beta [Hanashima, Mizushina, Ohta, Yamazaki, Sugawara and Sakaguchi (2000) Jpn. J. Cancer Res. 91, 1073-1083] and an immunosuppressive agent [Matsumoto, Sahara, Fujita, Shimozawa, Takenouchi, Torigoe, Hanashima, Yamazaki, Takahashi, Sugawara et al. (2002) Transplantation 74, 261-267]. The purpose of this paper is to elucidate the biochemical properties of the inhibition more precisely. As expected, SQDG could inhibit the activities of mammalian DNA polymerases such as alpha, delta, eta and kappa in vitro in the range of 2-5 micro M, and beta and lambda in vitro in the range of 20-45 micro M. However, SQDG could inhibit only mammalian DNA polymerases epsilon (pol epsilon) activity at less than 0.04 micro M. SQDG bound more tightly to mammalian pol epsilon than the other mammalian polymerases tested. Moreover, SQDG could inhibit the activities of all the polymerases from animals such as fish and insect, but not of the polymerases from plant and prokaryotes. SQDG should, therefore, be called a mammalian pol epsilon-specific inhibitor or animal polymerase-specific inhibitor. To our knowledge, this represents the first report about an inhibitor specific to mammalian pol epsilon.

Positional distribution of fatty acids on the glycerol backbone during the biosynthesis of glycerolipids in Ectocarpus fasciculatus

The biosynthesis of glycolipids in E. fasciculatus was studied by 14C label and chase. The fatty acids in sulphoquinovosyl diacylglycerol (SQDG) were almost 16-carbon and 18-carbon ones. In addition to the two fatty acids, monogalactosyl diacylglycerol (MGDG) and digalactosyl diacylglycerol (DGDG) contained 8.5 mol% and 31.0 mol% of eicosapentaenoic acid (20: 5), respectively, and this fatty acid was usually distributed in the sn-1 position of the glycerol backbone. When plants were incubated with [2−14C] acetate, differences existed in the positional distribution of the labeled fatty acids in sn-1 and sn-2 among the three glycerolipids. In SQDG., 14C-labeled fatty acids were distributed uniformly in the sn-1 and sn-2 positions. In DGDG., 14C-labeled fatty acids were mainly distributed in the sn-2 position. In MGDG., the radioactivity of fatty acids in sn-1 position was far greater than that in sn-2 position after a 30 min pulse label, and the difference in radioactivity between the two positions decreased rapidly. The above results indicated that differences in the positional distribution of 14C-labeled fatty acids between sn-1 and sn-2 positions might be related to 20: 5 and the biosynthesis of DGDG. Our results also suggested that E. fasciculatus had the same DGDG biosynthetic pathway as that in higher plants and galactosyl transferase was selective for MGDG.

Bleaching herbicide effects on plastids of dark-grown plants: lipid composition of etioplasts in amitrole and norflurazon-treated barley leaves.

The effects of the bleaching herbicides amitrole (125 micro M) and norflurazon (100 micro M) on etioplast lipids were studied in barley plants (Hordeum vulgare L. cv. Express) grown for 7 d either at 20 degrees C or 30 degrees C in darkness. Total lipid, glycolipid and phospholipid contents of control etioplasts were increased at 30 degrees C in comparison with those at 20 degrees C. The two herbicides caused a decrease in the total lipid, glycolipid and phospholipid amounts compared to the untreated etioplasts and lowered the lipid to protein ratio. In the controls, monogalactosyldiacylglycerol (MGDG) and digalactosyldiacylglycerol (DGDG) accounted for about 66 mol% of the etioplast polar lipids, while the remainder was represented by sulphoquinovosyldiacylglycerol (SQDG) and phosphatidylglycerol (PG), in approximately equal proportions. Both amitrole and norflurazon increased MGDG at both temperatures, but decreased DGDG except with norflurazon at 30 degrees C. As a consequence, the MGDG to DGDG molar ratio was higher in the herbicide-treated etioplasts compared to the controls at both the growth temperatures. The amount of the negatively charged polar lipids SQDG and PG were decreased by treatments with amitrole at 20 degrees C and norflurazon at 30 degrees C. The two herbicides determined different responses in the fatty acid unsaturation of the individual polar lipids. Changes in the lipid composition of etioplasts and the interaction between the pigment-protein complex, protochlorophyllide-NADPH-protochlorophyllide oxidoreductase, and polar lipids are discussed.

Sulpholipid reflects plant resistance to stress-factor action

The results of studying the behaviour of sulphoquinovosyldiacylglycerol (SQDG) during environmental-factor action are discussed. Low-temperature action caused SQDG accumulation in 1-year-old shoot bark of resistant apple varieties, and was especially striking in the Siberia apple, a variety of extreme hardiness. Another unfavourable factor, high temperature, induced SQDG accumulation in leaves and chloroplasts of drought-resistant plants while in sensitive plants a decrease in its content took place. Water deficit caused the same effect. SQDG increase was also observed in field experiments with artificial irrigation in the drought region of Ukraine, at the stages of stooling and milk ripeness. Besides, SQDG accumulation was observed in wheat plants infected by Puccinia graminis and kidney bean plants infected by tobacco mosaic and potato x viruses. But lead supplied at various concentrations caused an SQDG decrease in wheat seedling leaves and roots. The reasons for these phenomena seemed to be mainly the stabilization of the photosynthetic processes, particularly ATP synthesis and light-harvesting complex II functioning, but signal function also cannot be excluded.

Environmental effects on acidic lipids of thylakoid membranes

The contents of the chloroplast acidic lipids, SQDG (sulphoquinovosyldiacylglycerol) and PG (phosphatidylglycerol), were reduced in the cells of Chlamydomonas reinhartdtii with exposure to sulphur- or phosphorus-source limitation, respectively. The decrease in the content of one acidic lipid was accompanied by an increase in the content of the other acidic lipid, which resulted in the maintenance of a certain level of total acidic lipids of chloroplast membranes. On the other hand, the content of each acidic lipid was little affected by temperature stresses during cell growth.

Environmental effects on acidic lipids of thylakoid membranes

The contents of the chloroplast acidic lipids, SQDG (sulphoquinovosyldiacylglycerol) and PG (phosphatidylglycerol), were reduced in the cells of Chlamydomonas reinhartdtii with exposure to sulphur- or phosphorus-source limitation, respectively. The decrease in the content of one acidic lipid was accompanied by an increase in the content of the other acidic lipid, which resulted in the maintenance of a certain level of total acidic lipids of chloroplast membranes. On the other hand, the content of each acidic lipid was little affected by temperature stresses during cell growth.

Dependence of the lipid composition of Spirulina platensis (Nordst.) Geitl. on the mode of energy existence of cultures: from photoautotrophy to photoheterotrophy

Dependence of the lipid composition of the blue-green alga Spirulina platensis (Nordst.) Geitl. on the mode of energy existence of cultures was studied. It has been shown that under conditions providing the possibility of alga's transfer to photoheterotrophy, the culture growth rate essentially increased, which was accompanied by some inhibition of the lipid accumulation process. It has been found that the content of monogalactosyldiacylglycerol (MGDG) was twice as low, that of sulphoquinovosyldiacylglycerol (SQDG) was thrice as low, while the content of phosphatidyl-glycerol (PG) was more than 1.7 times as high as in the control. Under conditions favorable for photoheterotrophy, the values of DGDG (digalactosyldiacylglycerol) ratios have increased. The revealed modifications in the composition of polar lipids evidence for a structural reorganization of the membrane apparatus in S. platensis due to changes in carbon nutrition conditions. Such structural reconstructions may provide some correction of the lipid matrix flowability and the sufficient stabilization level of proteins in electron-transport chains, pigments, and enzymes.