Environments with elevated antimony (Sb) levels often result from industrial and mining activities, causing harm to ecosystems due to the inherent toxicity. Traditional treatments aim to eliminate or decrease Sb toxicity, yet they often result in the generation of secondary pollution. This study explores a novel approach for enhancing biological sulfate-reduction by biochar addition to cope with high Sb concentrations. Moreover, this research postulates sulfate-reduction bioprocess as a feasible alternative to obtain valuable antimony sulfide. The findings indicate that sulfate reduction effectively removes antimony, particularly at high concentrations, using a non-specialized inoculum. Biochar plays a pivotal role in enhancing sulfate-reduction rates, reducing lag-phase periods, and enriching the microorganisms responsible for sulfate reduction.Interestingly, lower concentrations of Sb(III) (5 to 40mg/L) and Sb(V) (5 to 100mg/L) exhibited a lower removal percentage compared to higher concentrations of Sb(III) (80 to 500mg/L) and Sb(V) (200 to 1000mg/L). This phenomenon was attributed to the excess sulfide hindering the equilibrium of the first-order reaction at lower concentrations, while at higher concentrations, the reaction proceeded more rapidly. A combination of biochemical and physicochemical reactions facilitated the removal of >95% Sb(III) and >97% Sb(V) using biochar.Furthermore, various taxa displayed a significant logarithmic rate of change in their abundance, influenced by the application of biochar or the introduction of distinct antimony species. The biogenic sulfide generated through the sulfate reduction process reacted with the Sb species, resulting in the precipitation of stibnite (Sb2S3), Na3SbS3, and Na3SbS4. These precipitates are considered crucial precursors in electronic applications.In conclusion, this study constitutes a pioneering exploration of elevated antimony (Sb) concentrations under sulfate-reducing conditions, significantly contributing to an advancement of knowledge in the application of biological processes.