Sugarcane Genotypes Research Articles

Disclosure of Productive and Stable Sugarcane Genotypes for Cane Yield and Quality Traits Using Various Stability Statistics

Disclosure of Productive and Stable Sugarcane Genotypes for Cane Yield and Quality Traits Using Various Stability Statistics

Evaluation of the quantitative and qualitative traits of elite sugarcane genotypes in the peninsular zone of Maharashtra under varying fertilizer levels

Evaluation of the quantitative and qualitative traits of elite sugarcane genotypes in the peninsular zone of Maharashtra under varying fertilizer levels

Water Use Efficiency Characteristics and Their Contributions to Yield in Diverse Sugarcane Genotypes with Varying Drought Resistance Levels Under Different Field Irrigation Conditions

Drought is the major abiotic constraint affecting sugarcane productivity and quality worldwide. This obstacle may be alleviated through sugarcane genotypes demonstrating good water use efficiency (WUE) performance. This study aims to investigate the WUE characteristics of various sugarcane genotypes under different soil water availability levels. Plant and ratoon field experiments were conducted using a split-plot randomized complete block design with three replications. The main plots were assigned three types of irrigation: (1) rainfed conditions (RF), (2) field capacity conditions (FC), and (3) half-available water (½ AW). The subplots consisted of six sugarcane genotypes with varying levels of drought resistance, i.e., KK3, UT13, Kps01-12, KKU99-03, KKU99-02, and UT12. Data on yield, stalk numbers, stalk diameter, height, and WUE were collected throughout the crop cycle for both plant and ratoon crops. For the plant crop, the net photosynthesis rate, transpiration rate, stomatal conductance, and leaf area index (LAI) were recorded during the crop period. In both plant and ratoon crops, the WUE in the RF treatment was lower than in the FC and ½ AW treatments during the drought stress period 4 months after planting (MAP). In the recovery phase, the WUE in the ½ AW treatment fell between the FC and RF treatments. The RF treatment exhibited the lowest WUE compared to the other two water regime treatments at the maturity stage. The drought-resistant genotypes KK3 and UT13 maintained high WUE values throughout both the drought and recovery periods and exhibited outstanding LAIs at 4 and 6 MAP. A significant relationship existed between WUE and LAI during these periods. Moreover, WUE was positively correlated with cane yields and yield components, such as stalk weight, shoot diameter, and height, during recovery and tiller number and height during ripening. Therefore, WUE and LAI are efficient parameters for supporting and maintaining growth and yield during water deficit and recovery phases under rainfed conditions.

Genetic Variability and Flowering Intensity of Sugarcane Genotypes Affected by Agro-Climatic Conditions of Alexandria

Genetic Variability and Flowering Intensity of Sugarcane Genotypes Affected by Agro-Climatic Conditions of Alexandria

Carbon Isotopic Composition Reflects Intrinsic Water Use Efficiency But Not its Component Traits in Sugarcane

Water is the most important resource in plant growth and is a major limiting factor in sugarcane productivity worldwide. Improving water use efficiency (WUE) can increase sugarcane productivity relative to available water resources by increasing photosynthetic capacity relative to transpiration and stomatal conductance instead of decreasing stomatal conductance. Leaf carbon stable isotopic composition (δ13Cleaf) can serve as a proxy for intrinsic WUE (WUEi) because WUEi and δ13Cleaf are theoretically related through the link between intracellular and ambient CO2 concentrations (Ci/Ca) and leaf CO2 discrimination (Δ13Cleaf). In this study we surveyed 55 sugarcane genotypes for WUEi, leaf WUE (WUEleaf), Ci/Ca, and δ13Cleaf by gas exchange measurements and stable isotope analysis. We hypothesized that significant genotypic variation was found in WUEi, WUEleaf, and δ13Cleaf within the sugarcane population in Louisiana. We also hypothesized that both WUEi and δ13Cleaf and Δ13Cleaf and Ci/Ca were correlated and that δ13Cleaf could be used as a proxy for WUEi in sugarcane. Here WUEi and WUEleaf had a genetic effect and were controlled mostly by water loss (stomatal conductance or transpiration). WUEi, WUEleaf, Ci/Ca, and δ13Cleaf were correlated, but δ13Cleaf was not correlated with the component traits of WUEi (photosynthetic rate and stomatal conductance). δ13Cleaf shows promise as a proxy for WUEi to at least be able to select the tails of the distribution, but the relationship between WUEi and δ13Cleaf may not be sufficiently strong to select WUE at a finer scale.

Field Evaluation of Sugarcane Genotypes for Resistance to Leaf Scald Disease in China

Field Evaluation of Sugarcane Genotypes for Resistance to Leaf Scald Disease in China

Influence of Spirulina Extract on Physiological, Qualitative, and Productive Traits of Four Sugarcane Genotypes

This study was conducted at El-Sabahia Research Station (latitude 31°12′ N, longitude 29°58′ E) in Alexandria, Egypt to evaluate the effect of Spirulina platensis algae extract on the growth, yield, and juice quality of four sugarcane genotypes during the 2020/2021 and 2021/2022 seasons. The sugarcane genotypes tested (G.T. 54-9, G. 2003-47, G. 84-47, and G. 2004-27) were treated with four concentrations (0, 0.1, 0.2, and 0.3%) of spirulina algae extract (SE) during their development as plant cane and first ratoon crops. At harvest, the growth, physiological, and juice quality characteristics were documented, while relative chlorophyll content was measured 210 days after sowing. Spraying canes with 0.2% of SE was the most effective treatment in enhancing all of the evaluated characteristics compared to those left without SE. Cane yield was more closely correlated with stalk weight (r = 0.88), followed by leaf area index (r = 0.82), relative chlorophyll content (r = 0.82), stalk length (r = 0.76), and number of tillers (r = 0.73), while recoverable sugar yield was closely correlated (p < 0.01) with sugar content % (r = 0.76).

Identification of candidate single-nucleotide polymorphisms (SNPs) and genes associated with sugarcane leaf scald disease

Leaf scald, caused by Xanthomonas albilineans, is a severe disease affecting sugarcane worldwide. One of the most practical ways to control it is by developing resistant sugarcane cultivars. It is essential to identify genes associated with the response to leaf scald. A panel of 170 sugarcane genotypes was evaluated for resistance to leaf scald in field conditions for 2 years, followed by a 1-year greenhouse experiment. The phenotypic evaluation data showed a wide continuous distribution, with heritability values ranging from 0.58 to 0.84. Thirteen single nucleotide polymorphisms (SNPs) were identified, significantly associated with leaf scald resistance. Among these, eight were stable across multiple environments and association models. The candidate genes identified and validated based on RNA-seq and qRT-PCR included two genes that encode NB-ARC leucine-rich repeat (LRR)-containing domain disease-resistance protein. These findings provide a basis for developing marker-assisted selection strategies in sugarcane breeding programs.

Resistance of two sugarcane genotypes towards Sporisorium scitamineum Scoring Using Novel Method on Sugarcane buds tissue and Phenol Plant dynamics

Smut disease caused by a basidiomycete fungus, Sporisorium scitamineum, is one of major diseases on sugarcane worldwide. The disease could cause considerable yield losses up to 60% on susceptible varieties. Understanding of resistance characteristics of sugarcane clones is essential to support development of resistant sugarcane varieties to smut fungus infection. This study aimed to determine characteristics of sugarcane genotypes J19R17-325/15/3-9 and ASA Agribun toward S. scitamineum infection and the role of phenolic compound on defense mechanism of sugarcane to smut infection. The research consisted of four treatments and was arranged according to the randomized block design with five replicates. The results revealed that J19R17-325/15/3-9 produced higher phenolic compound (0,192 mg/gr) rather than ASA Agribun (0,182 mg/gr). In addition, the distribution of fungal mycelia within sugarcane tissues on J19R17-325/15/3-9 was also lower than those on ASA Agribun. These results indicated that J19R17-325/15/3-9 might have resistance properties to S. scitamineum infection.

Evaluation of sugarcane genotypes (Saccharum sp. hybrid) for multi-trait stability analysis across diverse environments

The current study evaluated a total of 21 sugarcane genotypes across 27 diverse environments, including nine locations over three cropping seasons, to identify the superior and stable sugarcane genotypes. The pooled ANOVA results showed substantial genotype-environment interactions among the 21 genotypes under study. The genotype Co 11015 exhibited the highest mean value for CCS yield, CCS percentage, and sucrose percentage. With the help of GGE analysis and biplots, both stable genotypes as well as mega environments were identified. The following genotypes such as Co 13014, Co 11015, Co 13018 and Co 14016 emerged as the high-mean performers and exhibited stability across diverse environments. The outcomes from ‘which-won-where’ patterns suggested the existence of different mega environments which are classified as ME1, ME2, and ME3. When the genotypes were ranked, it revealed that Co 14016 is closer to the ideal genotype, followed by Co 13014, Co 11015, and Co 13018 as the most desirable genotypes. Similarly, the environments E8, E25, and E26 were identified as the most desirable ones, based on GGE biplot environment rankings. The Multi-Trait Stability Index (MTSI) values highlighted the following genotypes such as Co 13018, Co 13003, and Co 11015 as stable ones with high performance across the traits and environments. The MGIDI (Multi-trait Genotype Ideotype Distance Index) values inferred that Co 11015, Co 15007, and Co 13018 are the superior genotypes. Consequently, Co 11015 was released for commercial cultivation based on its stability and superior performance for biomass yield and quality traits across the locations and environments.

Endophytic Beauveria bassiana in sugarcane and associated Eldana saccharina host plants in South Africa

Eldana saccharina Walker is a problematic sugarcane stalk borer pest in South Africa. Twenty-eight sugarcane genotypes (Saccharum spp. hybrids) and seven different species of host plants of E. saccharina were surveyed for the presence of endophytic Beauveria bassianasensu lato (s.l.). Collectively, 326 plant samples (roots, stems, or leaves) were collected from five locations in sugarcane-producing areas (KwaZulu-Natal; South Africa). Following plant surface disinfection, 128 fungal colonies morphologically resembling Beauveria spp. were isolated from internal plant tissues. The sequence analysis of the internal transcribed spacer region confirmed that 13 representative isolates were B. bassiana.s.l. Eight B. bassiana isolates were recovered from E. saccharina natural host plants, and 120 from different sugarcane stem parts (top node, top internodes, bottom node, and bottom internode). Endophytic B. bassiana isolates were recovered from 22 of 28 sugarcane genotypes surveyed. The number of B. bassiana isolates obtained from different sugarcane parts differed significantly, with more B. bassiana isolates obtained from the top than the bottom internodes. Furthermore, sugarcane genotypes N31 and N41 yielded B. bassiana from all plant parts sampled, while five sugarcane genotypes harbored no B. bassiana isolates. This study demonstrates for the first time the natural occurrence of endophytic B. bassiana strains in sugarcane plants and E. saccharina host plants in South Africa.

Exploring Potential Surrogate Systems for Studying the Early Steps of the Sporisorium scitamineum Pathogenesis.

Despite its global importance as a primary source of table sugar and bioethanol, sugarcane faces a significant threat to its production due to diseases. One of these diseases, sugarcane smut, involves the emergence of a whip-like structure from the host apical shoot. The slow onset of this pathogenesis is the most substantial challenge for researchers to investigate the molecular events leading to resistance or susceptibility. In this study, we explored the early interaction between the smut fungus Sporisorium scitamineum and foliar tissues of the model plants Arabidopsis thaliana and Nicotiana benthamiana. Upon inoculation with the fungus, A. thaliana showed a compatible reaction, producing lesions during fungus colonization, whereas N. benthamiana showed signs of nonhost resistance. In addition, we propose a sugarcane detached leaf assay using plants cultivated in vitro to reveal sugarcane smut response outcomes. We used two sugarcane genotypes with known contrasting reactions to smut in the field. Although there is no evidence of sugarcane smut fungus infecting host leaves naturally, the sugarcane detached leaf assay enabled a rapid assessment of disease outcomes. Different symptoms in the detached leaves after inoculation distinguished smut-susceptible and smut-resistant sugarcane genotypes. Microscopic observations and gene expression analysis of S. scitamineum candidate effectors confirmed the fungal growth and its restriction on the compatible and incompatible interactions, respectively. These findings offer new prospects into the disease phenotyping of S. scitamineum, which could greatly expedite the comprehension of the initial stages of the pathogenesis and predict smut resistance in sugarcane genotypes.

Phenotypic characterization and diversity estimates in sugarcane (Saccharum spp. hybrid) germplasm

ABSTRACT The aim of the study was to identify genotypes with superior phenotypic performance, level and pattern of phenotypic diversity, and major contributing traits to the diversity of the test materials. Sixteen phenotypic traits of 81 sugarcane genotypes were analyzed using univariate and multivariate statistical methods. The genotypes were planted across two locations using a partially balanced lattice design with two replications. The study depicted the existence of substantial variation among the test materials in all the studied traits. The selected top 5% clones for cane yield, sucrose content and sugar yield were as follows: B694–11, B658–10, B707–1 and B572–1; B552–11, B498–9, B552–21 and B564–1; and B552–11, B549–10, B658–10 and B572–1, respectively. Sugarcane genotypes of clusters V and VII were the most divergent as demonstrated by their greatest intercluster distances, while sugar yield, cane yield and recoverable sucrose percent were the major contributors for the diversity of the test materials as revealed by PCA based on all of the studied traits. This suggests that the most divergent genotypes could be used as donor for hybridization, while those with greater mean values of the major contributing traits could be targeted for selection breeding.

Yield and Multivariate Analysis among Twelve Sugarcane (Saccharum Officinarum L) Genotypes at Mankusa, North Western, Ethiopia

Smallholders cultivated sugarcane for household consumption, immediate cash, and feeding livestock in Ethiopia. However, its production and productivity are constrained by a lack of improved varieties. Sugarcane germplasm was collected from Districts of West Gojjam, Ethiopia, to evaluate yield and its contributing characters and quantify the phenotypic diversity at Mankusa, Jabitehnan, Ethiopia. The experiment was conducted using a randomised complete block design with three replications for two crop cycles, from April 2020 to March 2023. Data were recorded for the number of tillers, internode length, sellable stalks, plant height, stalk diameter, cane yield and biomass yield from twelve genotypes collected. The data recorded were subjected to analysis of variance, and means were separated using the Duncan Multiple Range Test at a 5% significance level. Variance and multivariate analysis indicated the existence of high phenotypic diversity between genotypes in all quantitative traits studied. Acc 7/20 and Acc 5/20 genotypes produced the highest number of tillers and longest internodes, while Acc 6/20 produced the highest number of sellable stalks. The genotype Acc 4/20 was the longest, and the thickest genotype, which could be elite donors for it respected quality traits through crossing. The highest cane yield was recorded from Acc 4/20 (178.04 t/ha), Acc 7/20 (151.41 t/ha), Acc 8/20 (134.1 t/ha) and Acc 12/20 (132 t/ha), producing 58, 34, 19 and 17% advantageous from the overall genotypes mean, respectively. Cluster analysis grouped the twelve sugarcane genotypes into four clusters, indicating the possibility of broadening the genetic basis by crossing genotypes in the different clusters. It also indicated that a cross between cluster II and III genotypes could create the thickest canes with many sellable stalks. Therefore, genotypes Acc 04/20, Acc 07/20, Acc 8/20 and Acc 12/20 have been recommended and must be evaluated in other sugarcane growing areas to exploit their potential.

Occurrence and field evaluation of promising sugarcane genotypes for resistance to midrib red rot disease in China

AbstractSugarcane midrib red rot caused by Colletotrichum falcatum is widespread in China. Standardizing the resistance evaluation methods and screening resistant varieties against this disease is crucial. C. falcatum was determined as the causal agent of midrib red rot by isolating and identifying pathogenic fungi from diseased leaves in Guangxi, China. A field trial of 237 sugarcane genotypes was conducted for three consecutive years to investigate disease incidence and indices for three experimental sites in Guangxi. The combined analysis of variance showed that disease incidence was significantly affected by genotype, year, location and their interactions. Cluster and discriminant analysis showed that 237 sugarcane genotypes were classified into five categories, including 20 as highly resistant, 82 as resistant, 71 as moderately susceptible, 46 as susceptible and 18 as highly susceptible. This study has enhanced our understanding of sugarcane resistance to midrib red rot, selected disease‐resistant parents and laid the foundation for developing elite disease‐resistant varieties.

Determination of Morpho-Physiological Traits for Assessing Drought Tolerance in Sugarcane.

Drought is a significant constraint to sugarcane productivity. Therefore, understanding how different varieties of sugarcane respond to drought stress can facilitate breeding programs and set up criteria for selecting drought-tolerant varieties. In the present study, we examined eight morpho-physiological traits to distinguish 40 sugarcane genotypes categorized into four groups based on significant differences in cane yield under non-stressed conditions and reduction of cane yield under drought-stressed conditions. The study was conducted during the formative stage in a greenhouse, encompassing both control and drought conditions. Drought treatments resulted in significant changes and differences in the mean values of various morpho-physiological traits. The hierarchical clustering analysis, utilizing stay-green traits such as higher chlorophyll fluorescence ratio (Fv/Fm), leaf chlorophyll content (SPAD), leaf relative water content (RWC), and lower leaf rolling score (LR), leaf drying score (LD), and drought recovery score (DR), successfully grouped 40 sugarcane genotypes into four major clusters, similar to the previously categorized groups. Correlation analysis showed significant relationships among cane yield, reduction of cane yield under drought conditions, and the stay-green traits. Our results demonstrated that morpho-physiological traits contributing to the "stay-green" phenotypes could be useful as selection criteria for drought tolerance in sugarcane.

GGE Biplot Analysis of Exotic Sugarcane Genotypes in Major Sugarcane Producing Agro-ecologies of Ethiopia

GGE Biplot Analysis of Exotic Sugarcane Genotypes in Major Sugarcane Producing Agro-ecologies of Ethiopia

ASSESSMENT OF RATOONING POTENTIAL IN VARIOUS PROMISING STRAINS OF SUGARCANE UNDER SOUTH PUNJAB CONDITIONS

The present study aimed to assess the ratooning potential of various sugarcane strains at the Sugarcane Research Station Khanpur during the 2019-20 period. The selected promising sugarcane genotypes, including S2013-US-917, S2014-SL-1359, S2014-SL-2200, PSR-07-45, S2015-SL-89, S2015-SL-289, S2015-SL-404, S2015-SL-444, CPF-253, and HSF-240 (check), were subjected to experimentation. The research followed a Randomized Complete Block Design (RCBD) with three replications. Significant variations in ratooning performance were observed among the studied genotypes. Notably, the strain S2015-SL-444 emerged as the most promising, exhibiting the highest cane yield at 119.80 tons per hectare and excellent stubble sprouting with 1.64 sprouts per plant. Additionally, this top-performing strain demonstrated good quality, registering a field brix of 19.55%. Although the highest field brix (21.65%) was recorded in S2013-US-917, S2015-SL-89 closely followed with a field brix of 21.24%. S2015-SL-444, the standout sugarcane clone, outperformed the check by producing 14.02 tons more ratoon cane yield per hectare. Therefore, it is recommended that the genotype S2015-SL-444, with its exceptional ratooning potential, be cultivated in the agro-ecological climatic conditions of South Punjab for ratoon crop cultivation

Genetic variability, character association and path analysis in sugarcane genotypes

ABSTRACT The study, conducted at Tana-Beles Sugarcane plantation in Ethiopia from May 2022 to October 2023, used a partially balanced lattice design with two replications to assess variability and trait associations among 196 sugarcane genotypes collected from 14 countries. Highly significant (p < 0.0001) variability was observed for all traits; especially genotypes with fuzz exhibited wider variation for cane (20.97–135.31 t/ha) and sugar yield (1.83–20.42 t/ha), highlighting their potential for improvement. Except for single cane weight, brix, and purity, all traits displayed moderate to high heritability (H2 : 31–79%) and genetic advance (GAM: 10.69–53.14%), indicating potential for improvement through phenotypic selection being controlled by additive gene actions. The number of millable stalks, stalk length, stalk diameter, brix and cane yield showed significant positive genotypic correlations (r = 0.49, r = 0.55, r = 0.41, r = 0.65, and r = 0.98, respectively) and strong direct positive effects (0.33, 0.47, 0.75, 0.51 and 0.83, respectively) on sugar yield. Thus, Sugar yield, along with these traits, can serve as selection criteria for identifying high-sugar-yielding sugarcane genotypes. Consequently, the top three sugarcane genotypes for multi-traits, namely B552–11, FG04–466, and B707–1, could be evaluated across seasons for commercial use at Tana-Beles.

Genetic Variability and Clustering Patterns of Sugarcane (Saccharum spp.) Germplasms with Respect to Sucrose-Related Traits

Fifty-five sugarcane genotypes from around the world were collected and evaluated for potential use as parental material in the USDA ARS Canal Point (CP) sugarcane breeding program in Florida, USA. The genotypes were planted in a trial with four check cultivars on organic soils with four replications, and data were collected for two years [i.e., plant cane (PC) and first ratoon (FR) crops] to assess sucrose-yield-related traits and the cane-yield-related traits in PC. Using a multivariate analysis, variation was observed in all cane—[i.e., stalk weight, stalk population and cane yield] and sugar-yield-related traits [i.e., Brix, Pol, sucrose content and commercial recoverable sucrose (CRS)]. The mean CRS content was greater in the FR crop than the PC crop. Significant variations were attributed to genotype (G), crop cycles (C) and G × C effects. Variations between crop cycles were highly significant for all sucrose yield components, which could complicate the downstream selection of genotypes for sucrose yield. Based on CRS content, genotypes could be grouped into six distinct clusters. Based on plant cane data, cane yield traits (stalk weight, stalk population and cane yield) were used to estimate the breeding values of parents. Of the 55 genotypes, 8 had significantly greater t-BLUP values for cane yield, along with CP 00-1101. Combined sucrose yield traits, (Brix, Pol and sucrose content) from the two crops were used to estimate the breeding values of parents. Of the 55 genotypes, 10 genotypes had significantly greater t-BLUP values for CRS, along with CP 00-1101, CP 96-1252 and CP 01-2390, and can be considered as elite parents in future breeding efforts. These results provide a foundation for the efficient integration of genetic diversity in developing commercial cultivars, with improved sucrose yields, into the CP sugarcane breeding program.