Sufficient Material Research Articles

Evaluating parental experiences in teaching children with learning disabilities in resource rooms of public schools in Amman

BackgroundThe education of children with learning disabilities in public schools’ resource rooms remains a vital area of concern, particularly in Amman, where diverse educational needs and varying levels of resource allocation pose significant challenges. Despite the establishment of resource rooms, there is a notable lack of comprehensive data on parental satisfaction with these services. Previous research has indicated that the effectiveness of such educational settings largely depends on the quality of instruction, the adequacy of educational resources, and the extent of parental involvement and satisfaction. However, there is a significant gap in understanding how these factors specifically impact parents’ perceptions in the context of Amman’s public schools.AimThis study aimed to address this gap by evaluating the perspectives of parents of students with learning disabilities in resource rooms within Amman’s public schools, focusing on their satisfaction with educational services, including instruction quality, resource adequacy, and facility accessibility.MethodsA cross-sectional study design was utilized, involving a structured questionnaire administered to 200 parents of children attending resource rooms in 33 public schools across Amman. The questionnaire included Likert-scale questions and demographic queries to assess parents’ perspectives on the educational services provided. Data were analyzed using SPSS version 27 to compute descriptive statistics, correlation coefficients, and hierarchical linear modeling (HLM) to account for clustering effects.ResultsThe majority of respondents were female (60%, n = 120), and the largest age group was 31–40 years (45%, n = 90). Most parents held a Bachelor’s degree (50%, n = 100) and were employed (75%, n = 150). The predominant age group of the children was 11–15 years (50%, n = 100), with dyslexia being the most common type of learning disability (40%, n = 80). Parents rated the quality of instruction at a mean of 3.5 (SD = 1.2), adequacy of educational materials at 3.3 (SD = 1.1), and overall satisfaction with the program at 3.6 (SD = 1.0). Accessibility of facilities was rated lower at 2.8 (SD = 1.3). This interpretation is based on the lower mean score, which indicates a less favorable perception of accessibility, and the relatively high standard deviation, which reflects variability in parental experiences. HLM analysis revealed significant positive relationships between quality of instruction (β = 0.75, SE = 0.05, t = 15.00, p < 0.01), adequacy of educational materials (β = 0.70, SE = 0.04, t = 17.50, p < 0.01), and accessibility of facilities (β = 0.65, SE = 0.06, t = 10.83, p < 0.01) with overall parental satisfaction.ConclusionThe study underscores the importance of high-quality instruction, sufficient educational materials, and improved facility accessibility in enhancing parental satisfaction with resource room programs in Amman. This conclusion is supported by the findings from both the hierarchical regression analysis and the descriptive statistics, demonstrating that these factors are crucial for the effective support of children with learning disabilities.

Level of nurse–nurse collaboration and influencing factors in Türkiye and Italy: a mixed-methods study

Background: Collaboration between nurses is crucial in improving quality in healthcare and plays a key role in increasing nurses’ job satisfaction. Objectives: Our study aimed to investigate the level of collaboration between nurses working in Turkey and Italy and its related factors. The study also deepened the point of view of clinical nurses and nurse managers on this topic. Methods: This study was carried out as mixed-methods research including both qualitative and quantitative methods. The study’s quantitative sample included 348 clinical nurses. The Nurse–Nurse Collaboration Scale was used to collect quantitative data. Five open-ended questions were used to analyse the nurses’ point of view about intraprofessional collaboration. Individual interviews were conducted with 10 clinical nurses and 10 nurse managers. Results: Nurse–nurse collaboration was above the cut off value in both countries. However, significant inter-country differences were found in the collaboration subscales. Qualitative results emphasised that personal characteristics, a specific training in collaboration, the opportunities for comparison and mutual consultation during working shifts may affect the level of collaboration. Nurse managers argued that factors such as manpower planning, suitable working conditions, sufficient materials, empathy, job satisfaction, motivation and effective communication affect collaboration. Conclusion: Greater efforts should be made to implement collaboration between nurses in healthcare settings. Our results help healthcare services in implementing new collaboration policies.

Targeted microscopic rare cell retrieval yields sufficient deoxyribonucleic acid for molecular testing in fixed tissue with low tumor fraction

Abstract Introduction/Objective DNA extracted from formalin-fixed paraffin-embedded tissue (FFPE) blocks is often utilized for molecular oncologic testing. Cases with low tumor fraction often fail to obtain adequate tumor for molecular testing. We hypothesized that targeted retrieval of visualized tumor cells from FFPE sections, including those with low tumor fraction, could yield sufficient DNA for molecular testing. Methods/Case Report Unstained sections of various thicknesses were cut from low tumor fraction blocks and visualized by light microscopy and the RareCyte CyteFinder II (Seattle, WA). Microscopic cell retrieval was performed on the RareCyte using 40 µm diameter needles from identified regions of interest. Samples were deparaffinized using Zymo Research (Tustin, CA) solution, and digested and purified using Zymo-Spin Column technology. Sample DNA was quantified with NanoDrop Spectrophotometer (Chino, CA). Results (if a Case Study enter NA) Regions of interest were morphologically identifiable on 4-10 µm thick sections and harder to visualize on 12 µm thick sections. Targeted microscopic cell retrieval was performed with 40 µm diameter needles. Retrieval was performed from 26 to 34 regions of interest per sample and pooled for molecular testing. Samples (n=4) yielded a mean of 81.0 ng of DNA ± 18.4 ng (1.8 ng/µL ± 0.4 ng/µL) and a median of 87.75 ng (1.95 ng/µL). Conclusion Targeted microscopic cell retrieval from low tumor fraction FFPE slides using the RareCyte CyteFinder II can provide sufficient DNA material for molecular testing. Application of this technology has the possibility of recovering sufficient tumor DNA for molecular testing from specimens that may be deemed unsuitable for molecular testing according to current standards. We aim to focus on increasing sample size and completing comprehensive molecular analysis as we continue to expand this work.

The state of cancer care in Russia: pancreatic cancer (C25). Diagnosis, prevalence, quality of accounting, typical mortality. Part 1. (clinical and population-based research)

The study focuses on pancreatic cancer (C25), a malignant neoplasm arising from the ductal epithelium or glandular tissue of the pancreas, which accounts for 3% of the overall cancer incidence in Russia. The rates of incidence and mortality are rising, with the number of newly registered patients in Russia being lower than the number of recorded deaths, although the one-year mortality rate among patients is decreasing. The direct cause of pancreatic cancer (C25) remains unknown. Risk factors for developing this condition include genetics, smoking, alcohol abuse, a sedentary lifestyle, excessive meat consumption, and a lack of plant-based foods in the diet. Diseases associated with a high risk of this pathology include long-standing chronic pancreatitis, newly diagnosed type 2 diabetes in individuals aged 50-70, and cystic neoplasms of the pancreas. The aim of the study is to examine the patterns of incidence and mortality, the quality of patient registration, the characteristics of tumor localization, and the histological structure of pancreatic cancer (C25). The study will also track the nature of annual mortality among patients and explore the possibilities, methods, and techniques of modern diagnosis and treatment for these patients. The research confirmed the significant burden of pancreatic cancer (C25), primarily due to the late detection of this disease. The study provided sufficient material to analyze age-related risk factors, as well as the patterns of incidence and mortality in the population. It also examined and presented mortality rates among patients, including during the first year of observation.

Features of the use of unmanned aircraft during the inspection of the scene of the incident during the investigation of violations of the road safety rules or vehicle operation

The article highlights the procedural and tactical features of the inspection of the scene of a traffic accident with the use of an unmanned aerial vehicle. To achieve this goal, the forensic features of conducting an inspection of the scene during the pretrial investigation of crimes of violations of road safety rules or vehicle operation at each of the preparatory, working, and final stages are revealed. The current state and prospects of the use of unmanned aerial vehicles by the bodies and units of the National Police during the inspection of the scene of a traffic accident were studied. The advantages of using an unmanned aerial vehicle during the inspection of the scene of a traffic accident are given, the concept of an unmanned aerial vehicle is defined, the methods and tactical methods of using an unmanned aerial vehicle. Scientific and practical recommendations are given on the procedure for recording crimes with the help of an unmanned aerial vehicle regarding violations of the rules of road traffic safety or vehicle operation during the inspection of the scene of the incident. It was concluded that the use of an unmanned aerial vehicle in the activity of the investigator during the investigation of traffic accidents is legitimate and provides much more advantages than the use of other methods of recording, such as measuring with a manual or laser tape measure, taking photos or video recording with cameras or video cameras. The use of UAVs during the inspection of the scene of the incident significantly reduces the time of its inspection, while providing the opportunity to obtain high-quality and sufficient evidentiary material.

Approach to Validate ISPM-15 Compliance for Commercial Treatment Certification of Dielectric Standard Heating of Bulk Solid Wood Packing Materials using Radio Frequency

Abstract To substantially reduce the risk of alien invasive species moving to new geographic areas, phytosanitary treatment of wood packaging materials (WPM) in compliance with the International Standard of Phytosanitary Measures No. 15 (ISPM-15) is required by trading partners. Approved treatments include conventional heating, methyl bromide and sulfuryl fluoride fumigation, and dielectric heating (DH). The DH standard was officially adopted in 2013 but has not been practiced commercially due primarily to insufficient operational validation at commercial scale. In 2022, we converted our 50-kW radio frequency (RF) unit with a 1,200-board foot capacity from an oscillator electromagnetic field power generator to a solid-state power supply, which allows for selective power input adjustments during treatment; we also switched from a five-plate to a three-plate winged electrode system to improve heating uniformity. Each loading cycle can treat sufficient material to build ∼94 standard Grocery Manufacturers Association pallets. Our research team characterized the dielectric heating pattern and options for monitoring wood temperatures over a wide-ranging test matrix of WPM that varied by wood species, dimension, moisture content, and loading configuration. We found that this upgraded RF system markedly reduced treatment times and improved heating uniformity, allowing us to develop methods that can be used to verify compliance with ISPM-15 for improved technology transfer to industry. We also discuss the operational cost of RF treatment and make general cost comparisons to conventional heat treatment for WPM.

B-240 Multi-omic blood test for early detection of cancer and pre-cancer

Abstract Background The earlier diagnosis of cancer is vital to improve patient survival. Many liquid biopsy technologies are focused on single tumor-derived biomarkers, which limits test sensitivity, especially for early-stage cancers that do not shed sufficient genetic material into the bloodstream. The Dxcover liquid biopsy employs Multi-Omic Spectral Analysis (MOSA) that interrogates a blood sample with Infrared (IR) radiation and produces a distinctive signature that represents the whole biomolecular profile of the sample. This multi-omic blood test analyzes the full complement of tumor and immune-derived markers present within blood derivatives and could facilitate the earlier detection of cancer. The technology can be tuned for high sensitivity (rule out) or high specificity (rule in) depending on the desired application, clinical priorities and international healthcare markets. Methods Three clinical studies have been completed, assessing the technology for the detection of: (1) brain cancer, (2) multiple cancers, and (3) colorectal cancer (CRC) and pre-cancerous lesions. In the first study, we recruited 988 patients prospectively with non-specific symptoms associated with a brain tumor. Secondly, we carried out a large-scale multi-cancer evaluation (n = 2092 patients), targeting eight different cancers. Moreover, the CREATE (ColoREctal cancer & Adenoma Test Evaluation) feasibility study (3) examined 100 CRC, 92 adenoma samples and 104 colonoscopy screening control patients. Results In study 1, the brain cancer algorithm detected 96% of the patients with brain cancer, and 100% of glioblastomas, the most aggressive primary brain tumor. In the multi-cancer study (2), area under the receiver operating characteristic curve values were calculated for the eight cancer types versus symptomatic non-cancer controls: brain (0.90), breast (0.76), colorectal (0.91), kidney (0.91), lung (0.91), ovarian (0.86), pancreatic (0.84) and prostate (0.86). We also assessed the test performance when all eight cancer types were pooled to classify ‘any cancer’ against non-cancer patients. The cancer versus asymptomatic non-cancer classification detected 64% of stage I cancers when specificity was 99% (overall sensitivity 57%). When tuned for higher sensitivity, this model identified 99% of Stage I cancers (with specificity 59%). In the CREATE study, the test reported promising results for pre-cancer detection; at 90% specificity, the test reported sensitivities of 80% for CRC and 59% for advanced adenomas. Conclusions A rapid liquid biopsy that is sensitive to pre-cancerous lesions and early-stage cancer could substantially improve patient outcomes. There is a low barrier to integrating this blood test into existing diagnostic pathways as the technology is rapid, simple to use, and sample preparation is minimal. In addition, the spectroscopic liquid biopsy has the potential to be combined with other orthogonal tests, such as cell-free DNA, which could provide an efficient route to diagnosis. Cancer treatment can be more effective when given earlier, and this low-cost strategy has the potential to improve patient prognosis.

Circulation of avian Chlamydia abortus in the Netherlands and community-acquired pneumonia: an outbreak investigation and retrospective cohort study

In 2021, a novel group of Chlamydia strains in wild birds was classified as avian Chlamydia abortus, with unknown zoonotic potential. We report relevant features of avian C abortus infections from a Dutch family cluster and unrelated historical cases using clinical, epidemiological, and microbiological data. An outbreak of avian C abortus started in the Netherlands in December, 2022. Source investigation was done using questionnaires to interview patients and environmental sampling. The outbreak strain of avian C abortus was cultured from three patients from whom sufficient material was available for culture and underwent whole-genome analysis. The outbreak strains and retrospective cohort study strains previously submitted to the National Human Psittacosis surveillance programme in the Netherlands between 2010 and 2022 were typed by partial ompA sequencing. Strains with the same aberrant ompA genotype were further analysed with XerC gene plasmid analysis and compared with closely related Chlamydia sequences available in GenBank. An avian C abortus strain caused a cluster of respiratory illness in four family members. Three patients were hospitalised with community-acquired pneumonia, one of whom was admitted to the intensive care unit. The faeces of wild birds were considered a probable source for the index infection. For two family members, human-to-human transmission was a plausible route. Ten historical cases could be identified with avian C abortus with the same ompA genotype. All patients had been admitted to hospital, at least five developed pneumonia, and one died. This cluster supports that avian C abortus strains can cause human infections and underlines that human-to-human transmission should be considered when tracing the source of such infections. National Institute for Public Health and the Environment and Dutch Ministry of Agriculture, Fisheries, Food Security and Nature. For the Dutch translation of the abstract see Supplementary Materials section.

Mass media guidelines for sports concussion: a scoping review protocol

Sports concussion is a common and costly injury, and increased public injury awareness could help reduce costs. Many factors, including public messages about sports concussion by the sports media, shape...

The Impact of Nova Outbursts on the Chemical Abundance of the Interstellar Medium

Nova outbursts are the results of thermonuclear runaways, which occur when sufficient material accretes on the surfaces of white dwarfs (WDs). Using the MESA code, we construct a detailed grid for carbon-oxygen and oxygen-neon-magnesium novae. By employing population synthesis methods, we conduct a statistical analysis of the distribution of novae in the Milky Way. In our models, on average, a typical nova system may undergo about 8000 eruptions and the Galactic nova rate is ∼130 yr−1. The C, N, and O elements in nova ejecta are strongly affected by the mixing degree between WD core and accreted material. Our results show that the average value of 12C/13C in nova ejecta is about an order of magnitude lower than that on the surface of a red giant, that for 16O/17O is about 5 times lower, and that for 14N/15N is about 1.5 times lower. The annual yields of 13C , 15N, and 17O from nova ejection are larger than those from AGB stars. This indicates that compared to a red giant, nova eruptions are a more important source of the odd-numbered nuclear elements of 13C , 15N, and 17O in the Galactic interstellar medium.

MINIMALLY INVASIVE TREATMENT OF ACUTE INTESTINAL OBSTRUCTION BY PERITONITIS

Acute commissural intestinal obstruction aggravated by peritonitis has been proved to be quite common and severe disease which requires urgent operations; death rate of this disease averages 10,2 – 56,9 %. Taking into consideration these facts the aim of this work is to decrease risk factors of disease’s recurrence, to improve the level of prophylaxis of commissural intestinal obstruction aggravated by peritonitis using modern therapeutic methods. Clinical evaluation of 81 patients with commissural intestinal obstruction aggravated by peritonitis (OIOAP) who have been treated in our department for the last 8 years has been carried out in this investigation. On the base of sufficient clinical material, the following conclusions have been made: treatment by the means of endovideolaparoscopic method gives the opportunity not only to eliminate the cause of the development of commissural intestinal obstruction aggravated by peritonitis; it proved to be effective method of prevention of commissural disease after surgical operation; besides, this method completely meets the requirements of esthetic and cosmetic surgery. Actually this method prevents the recurrence of commissure formation, it is very important for patients with the tendency to commissure formation.

Investigating the influence of the Ag and Al co-doping in ZnO electron transport layer on the performance of organic-inorganic perovskite solar cells using experimentation and SCAPS-1D simulation

The sufficient material selection of electron transport layer (ETL) enormously promises exceptional conversion efficiency from perovskite (PVT) solar cells (PSCs), which in turn give rise to their rapid establishment in world-wide photovoltaic (PV) market. Among the tactics exerted on ETLs, and thus, intensifies the adequacy of associated PSCs, the methodology of doping stands productive. Therefore, this research validates the implementation of such effectual ETLs on widely approved methyl ammonium lead triiodide (MAPbI3)–based PSCs. The work brings together two versions of zinc oxide (ZnO) ETLs: one in its pristine form, and the other is aluminum (Al) co-doped with silver (Ag) represented as Ag-AZO. The investigation launches the PV capabilities of Ag-AZO ETL against its undoped correspondent. Accordingly, the earlier part of the investigation centers on the experimental assessment of ZnO and Ag-AZO nanoparticles (NPs) affirming their material and morphological aspects. Later, the research deals with the involvement of both NPs as ETLs signifying the PV potential of MAPbI3–based PSCs through comprehensive numerical analysis. The judgements of investigation declare that MAPbI3–based PSC with Ag-AZO ETL yields a desirable power conversion efficiency (PCE) of 27.26% against 25.98% from control cell. The investigation will definitely enlighten the development of forthcoming efficient PSCs incorporated with appropriate multiple metals co-doped ETLs.

ВНУТРИПОПУЛЯЦИОННАЯ ИЗМЕНЧИВОСТЬ ЭКСТЕРЬЕРНЫХ И КРАНИАЛЬНЫХ ПАРАМЕТРОВ СОНИ ЛЕСНОЙ DRYOMYS NITEDULA PALLAS, 1778 (GLIRIDAE, RODENTIA) В УСЛОВИЯХ СЕВЕРНОГО МАКРОСКЛОНА ЦЕНТРАЛЬНОГО КАВКАЗА

Based on the cariologically dated serial material on forest dormouse from the Central North Caucasian population population, we first conducted a comprehensive analysis of 45 animals (23 male and 22 females). The contrariety of the opinions in the issues of systematics (the number of intraspecific forms) and the lack of sufficient material in the scientific literature on Dryomys Nitedula Pallas, 1778, taking into account the high-profile structure of the mountain landscapes, led to this study. The intrapopulation variability of the Kariotip, 28 morphometric parameters of the body and skull, as well as 5 indices (the ratio of the tail length to the length of the body, the ratio of the zuloma width to the condilobasal skull length, the ratio of the lower jaw to the condylobasal skull length, the ratio of the width of the skull in the area of the auditory drums to the condylobasal were studied. The length of the skull, the ratio of the height of the skull in the area of the auditory drums to the width of the skull in the area of the auditory drums) taking into account the floor. In addition an attempt was made to conduct a comparative analysis of the most resistant to transformation of the skull (only 9 measures) between the West North Caucasian and Central North Caucasian populations due to the fact that animals from the the West North Caucasian population turned out to be a new subspecies (D.N. Heptneri Subsp. NOV.) Based on the analysis of mitochondrial (according to the Cytb) DNA. It should be noted that for all the body and skull seals we have studied, as well as indexes, there is no sexual dimorphism (t <3). In the conditions of the Elbrus version of the belt the Cariotic Sonya Lesnoy was studied for the first time (2n = 48, NF = 96). Allosome: X-chromosome is represented by an average submetententric, Y chromosome-the smallest element of the set. The karyotype turned out to be a persistent sign, is not subject to individual variability, respectively, has a high taxonomic weight.

Towards a Study of the Architectural Decoration of Public Buildings in Parion During the Early Roman Empire

Parion was one of the principal centers of the Roman province of Asia, and the remains of several public buildings adorned with intricate architectural decorations have been uncovered and partially explored. Among these structures are a theater, an odeon, and thermae, all dating to the Roman Empire. In addition to these edifices, other public buildings in the central part of Roman-era Parion remain undiscovered, raising im-portant questions about the organization of public space and the architectural character of the city’s core during the Early Empire. One of the most promising areas for further exploration is the “Agora” sector, bordered to the south by the odeon and to the north by the theater and thermae. This article offers an architectural and histori-cal analysis of the Corinthian order details found in this sector. The architectural elements discussed were un-covered during archaeological excavations conducted between 2015 and 2022 (Figs. 1-6). Although the availa-ble materials are insufficient to definitively identify the specific structures to which these decorative elements belonged, their forms, ornamentation, dimensions, and modules exhibit notable similarities to the architectural details of the southern façade of Hadrian’s Gate in Ephesus. This resemblance suggests a commonality in their architectural compositions and, by extension, the types of structures they adorned. Consequently, it is plausible to infer that these architectural elements were part of a portico associated with a building complex in the Agora. The design of this complex (or at least part of it) likely reflects Hadrian’s extensive building program in the Ro-man province of Asia.

Understanding stakeholders' perception on integrating ICT in teaching and learning science subjects: Experiences from Tanzanian secondary schools

This study investigated stakeholders’ perceptions of the integration of ICT in teaching and learning science subjects in secondary schools. A cross-sectional survey design, using a mixed approach, guided the study. Survey questionnaires and interviews were used for data collection. A total of 221 respondents, including students, teachers, heads of schools and district education officers, were sampled. The SPSS version 24 was used for quantitative data analysis. The qualitative data from the interviews were subjected to thematic analysis. Results indicate that stakeholders had positive perceptions of the benefits of integrating ICT in teaching science subjects. However, their positive perception was hampered by the accessibility of ICT materials, insufficient pedagogical knowledge and limited support from school management. Therefore, it affects the integration of ICT in teaching and learning science subjects. The study recommends government and management support to ensure sufficient ICT materials. Further, teachers should be equipped with sufficient pedagogical knowledge for ICT integration in teaching and learning science subjects.

Multiomics Profiling Distinguishes Sebaceous Carcinoma from Benign Sebaceous Neoplasms and Provides Insight into the Genetic Evolution of Sebaceous Carcinogenesis.

Sebaceous carcinoma is the third most common nonkeratinocyte skin cancer in the United States with 1,000 cases per year. The clinicopathologic features of sebaceous carcinoma and benign sebaceous neoplasms (adenomas, sebaceomas) can overlap, highlighting the need for molecular biomarkers to improve classification. This study describes the genomic and transcriptomic landscape of sebaceous neoplasms in order to understand tumor etiology and biomarkers relevant for diagnosis and treatment. We performed whole-genome sequencing (WGS) and whole-transcriptome sequencing (WTS) of sebaceous neoplasms from six academic and two federal healthcare facilities in the United States diagnosed between January 1, 1999, and December 31, 2021. We evaluated 98 sebaceous neoplasms: 64 tumors (32 adenomas, 2 sebaceomas, 5 atypical sebaceous neoplasms, 25 carcinomas) had sufficient material for WGS, 96 tumors (42 adenomas, 11 sebaceomas, 8 atypical sebaceous neoplasms, 35 carcinomas) had sufficient material for WTS, and 62 tumors (31 adenomas, 2 sebaceomas, 5 atypical sebaceous neoplasms, 24 carcinomas) had sufficient material for combined WGS and WTS. Overall, we found decreased cholesterol biosynthesis and increased TP53 mutations, copy number gains (chromosome 6, 8q, and/or 18), and tumor mutation burden-high (>10 mutations/MB) in carcinomas compared to adenomas. Although diminished compared to adenomas, most carcinomas still had higher cholesterol biosynthesis than nonmalignant skin. Multiomics profiling also supported a precancerous model of tumor evolution with sebaceomas and atypical sebaceous neoplasms being likely intermediate lesions. The study findings highlight key diagnostic biomarkers for sebaceous carcinoma and suggest that immunotherapy and modulation of cholesterol biosynthesis could be effective treatment strategies.

Development of an automated urban heritage monitoring tool: Between a techno-fix and fallacy

The preservation of urban heritage is one of the main challenges for contemporary society and an important task for governments. The administration of urban heritage preservation (UHP) requires effective methods, as well as sufficient material and human resources. The administrative practice of UHP is embedded in the relevant regulatory framework, which comprises legislative and normative acts at municipal, national, and international levels. The scale and range of heritage objects to be monitored, the complex statutory basis of the regulatory framework, and the traditionally low human and material resources of city administrations all contribute to the ineffectiveness of UHP practice in the UNESCO World Heritage Site of Vilnius.Advances in technologies such as 3D spatial scanning and AI-based image processing offer the promise of a technological fix to the ineffective administrative practice. In this paper, we present a grounded design study aimed at developing a technology prototype for an automated urban heritage risk monitoring tool. Originally conceived as a techno-fix to the inefficiency of administrative practice, the prototype development reveals tensions and stumbling blocks within digitalisation efforts. Using the analytical lens of the Trifecta model of IT-based regulation, the case study examines the interaction between technological and administrative domains in shaping technology design choices.Based on this case study, we critique the notion that IT is perceived as capable of providing “techno-fix” solutions to tasks requiring reflective human decision-making, especially when such solutions are expected to be effective without first redesigning the entire organisational system. The paper concludes with a discussion on the limits of automating administrative practices and identifies specific avenues for further research into the digitalisation of urban heritage preservation.

Detection and Molecular Characterization of GI-1 and GI-23 Avian Infectious Bronchitis Virus in Broilers Indicate the Emergence of New Genotypes in Bolivia.

Infectious Bronchitis Virus (IBV) is a major threat to the poultry industry worldwide, causing significant economic losses. While the virus's genetic structure is well understood, the specific strains circulating in Bolivia have remained uncharacterized until now. This study aimed to identify and characterize new IBV strains in Bolivia. Tissue samples from broilers exhibiting clinical signs of Infectious Bronchitis were screened to detect IBV using real-time RT-PCR (RT-qPCR). Positive samples with low cycle threshold (Ct) values were selected for sequencing the full S1 gene. Of the 12 samples analyzed, 10 were determined to be positive for IBV. However, only four samples yielded sufficient genetic material for sequencing and subsequent phylogenetic analysis. The results revealed the presence of GI-1 and GI-23 lineages, both belonging to genotype I (GI). The GI-1 lineage showed >99% sequence identity to the H120 and Massachusetts vaccine strains, suggesting a close relationship. In contrast, the GI-23 lineage clustered with other IBV strains, showing a distinct subclade that is genetically distant from Brazilian strains. No evidence of recombination was found. Furthermore, amino acid substitution analysis identified specific mutations in the S1 subunit, particularly in the hypervariable regions 1, 2, and 3. These mutations could potentially alter the virus's antigenicity, leading to reduced vaccine efficacy. The findings of this study highlight the importance of continued and broad genomic surveillance of circulating IBV strains and the need to improve vaccination strategies in Bolivia.

Effect of overlapping deposition strategy on microstructure and mechanical properties of Al-clad steel by AFSD

Cladding of the aluminum (Al) layer on steel was successfully implemented via additive friction stir deposition (AFSD). We revealed the relationship between the overlapping deposition strategy of AFSD and the resultant microstructure and mechanical properties of Al-clad steel for the first time. Overlapping deposition strategies efficiently improved the adhesion of the Al layer on steel in the boundary region owing to the sufficient material flow and large plastic deformation. This is in contrast to the sample without overlapping where the Al/steel interface in the boundary region is characterized by holes and weak bonding. In particular, the maximum shear strength (109.0 MPa) was obtained for the sample with an overlap of 8 mm, even higher than that of the single-pass sample. This study provided a novel approach to the fabrication of large-scaled clad layers on hard substrates, and this strategy is expected to greatly contribute to the adoption of AFSD in the fields of cladding, joining, and coating.

Fixative or Finish? Identifying the Surface Coating on John Constable’s Drawings Using Peptide Mass Fingerprinting

ABSTRACT A survey of graphite drawings by the English painter John Constable revealed that in many cases the artist applied a coating overtop of the media. This thin, clear, glossy coating was characterized on over thirty drawings where it appeared to be the same material although employed in different ways. Several attempts were made to identify the material with minimal success until peptide mass fingerprinting (PMF) was used, and a minimally invasive sampling method involving vinyl eraser cubes proved to be a very easy and effective technique to collect sufficient sample material for the analysis. The results of testing samples from seven drawings conclusively identified the coating as isinglass, which Constable was using in other preparations of support materials for painting. As part of this work, putative markers for the identification of isinglass by PMF were developed.