Community Succession Research Articles

Ecological dynamics explain modular denitrification in the ocean

Microorganisms in marine oxygen minimum zones (OMZs) drive globally impactful biogeochemical processes. One such process is multistep denitrification (NO3-→NO2-→NO→N2O→N2), which dominates OMZ bioavailable nitrogen (N) loss and nitrous oxide (N2O) production. Denitrification-derived N loss is typically measured and modeled as a single step, but observations reveal that most denitrifiers in OMZs contain subsets ("modules") of the complete pathway. Here, we identify the ecological mechanisms sustaining diverse denitrifiers, explain the prevalence of certain modules, and examine the implications for N loss. We describe microbial functional types carrying out diverse denitrification modules by their underlying redox chemistry, constraining their traits with thermodynamics and pathway length penalties, in an idealized OMZ ecosystem model. Biomass yields of single-step modules increase along the denitrification pathway when organic matter (OM) limits growth, which explains the viability of populations respiring NO2- and N2O in a NO3--filled ocean. Results predict denitrifier community succession along environmental gradients: Pathway length increases as the limiting substrate shifts from OM to N, suggesting a niche for the short NO3-→NO2- module in free-living, OM-limited communities, and for the complete pathway in organic particle-associated communities, consistent with observations. The model captures and mechanistically explains the observed dominance and higher oxygen tolerance of the NO3-→NO2- module. Results also capture observations that NO3- is the dominant source of N2O. Our framework advances the mechanistic understanding of the relationship between microbial ecology and N loss in the ocean and can be extended to other processes and environments.

Equipping Teachers for Success in Rural Communities: Unveiling the Key Characteristics and Attributes of Thriving Rural Teachers

Australian education faces persistent challenges in preparing and retaining teachers within rural contexts. This qualitative study examines the attributes of thriving rural teachers through focus group discussions with 103 rural educational leaders. Utilising a theoretical framework combining attribution theory and ecological systems perspectives, the study synthesised leaders’ perceptions of key dispositions and behaviours contributing to rural teachers’ success. Four interconnected dimensions emerged: being community, classroom, professionally and personally equipped. This study revealed that while individual teacher attributes are crucial, school support and community context significantly influence teacher success. The application of attribute theory further revealed that findings attributed teacher’s success to internal, stable, and controllable factors, suggesting that key traits could be developed through targeted professional development and experience. This study provides nuanced insights into ’rural readiness’, informing education policy decisions and practices, initial teacher education institutions, schools, and communities. The study emphasises the need for a holistic approach to teacher preparation and support that considers both individual and systemic factors.

Нелегальные связи между РПЦЗ и катакомбными общинами в СССР в 1960-е–1980-е гг.

The article analyses the situation of non-conformist Catacomb Orthodox communities in the USSR in the 1960s–1980s. It examines the problems of persecution by the Soviet regime against the church opposition, the loss of hierarchy with canonical apostolic succession in the catacomb communities, the emergence of various currents and groups, and the emergence of self-sanctification and sectarianism. In this context, special attention is paid to the establishment by the catacomb “True Orthodox Christians” (TOC) from the USSR of secret ties with the Russian Orthodox Church Outside of Russia (ROCOR), as well as to the attempts of the clergy of the Russian Church Abroad to regulate the canonical status of these catacomb communities and thereby prevent the spread of self-sanctification and degeneration into sectarianism in the catacomb environment by means of secret care (following the example of the “experience of the Edinoverie”). The situation of the Catacomb Church in the USSR and the restoration of its episcopate in the 1980s are examined in detail. The article is the first to introduce into scientific circulation previously unknown archival sources and eyewitness testimonies.The author expresses his particular gratitude to Archpriest Serafim Gan, head of the Chancery of the Synod of Bishops and secretary to the First Hierarch of the ROCOR for the opportunity to work with documents from the Archive of the Synod of Bishops of the ROCOR in New York.

10 years of primary succession in intertidal communities: specific and functional changes

10 years of primary succession in intertidal communities: specific and functional changes

Integrating justice-involved voices: the role of experiential knowledge in shaping reentry support programs

ABSTRACT Upon release from incarceration, system-impacted individuals in the U.S. face multifaceted challenges, with studies highlighting high rates of re-incarceration. Understanding the reentry process from the perspective of these individuals is imperative. Interviews with system-impacted and previously incarcerated persons underscore the importance of experiential knowledge in navigating reentry challenges. Through qualitative analysis, three categories of experiential knowledge emerged: Program Goals Informed by Experiential Knowledge, Utilization of Peers (Classes) as Experiential Knowledge, and Strategies for Understanding Personal Experience as Knowledge. These categories encapsulate the diverse ways in which experiential knowledge informs and shapes reentry support programs, showcasing both individual and collective successes across various stages of reintegration. This framework provides valuable insights into enhancing reentry programs, advocating for the integration of experiential knowledge to foster positive change and successful community reintegration. The article concludes by discussing how these interconnected themes offer a comprehensive understanding of how experiential knowledge shapes the reentry journey. By prioritizing the voices and experiences of system-impacted individuals, reentry programs can become more responsive, effective, and equitable, contributing to a more humane justice system.

Fermentation of cigar tobacco leaves with citrus flavonoids: changes in chemical, microbiological, and sensory properties

IntroductionFermentation plays a significant role in improving the quality of cigar tobacco leaves. Particularly, fermentation with characteristic additives has been identified as an effective approach to enhance the fermentation process. The objective of this study was to develop new additives and investigate their influence on cigar tobacco leaves.MethodsThe active ingredients extracted from three citrus species were obtained by ultrasonic-assisted extraction followed by solid phase purification. The effects of these ingredients as fermentation additives on the primary chemical components, microbial communities, and sensory quality of cigar tobacco leaves were subsequently analyzed.ResultsResults showed that: (1) Flavonoids were the main components of the prepared citrus extracts. (2) The addition of citrus extracts resulted in a reduced content of amino acids, oxalic acid, and unsaturated fatty acids in cigar tobacco leaves, while increasing the content of citric acid. Besides, the total amount of aroma substances in cigar tobacco leaves increased by 38.15% with the addition of citrus extracts, with notable enhancements in the levels of D-limonene, β-dihydroionone, dihydroactiniolactone, and other representative aroma components. (3) The addition of citrus extracts promoted the succession of the microbial community in cigar tobacco leaves and promoted the enrichment of Pseudomonas and Corynebacterium. (4) The addition of citrus extracts effectively reduced the irritation and improved the aroma richness of cigar tobacco leaves.DiscussionIn this study, the influence of citrus-derived active ingredients on cigar quality was systematically analyzed, providing a reference for the development of characteristic fermentation additives for cigars and the extension of cigar quality enhancement technologies.

Impacts of phosphate-solubilizing bacterium strain MWP-1 on vegetation growth, soil characteristics, and microbial communities in the Muli coal mining area, China

Due to the cold climate and low soil nutrient content, high-altitude mining areas are challenging to restore ecologically. Their poor nutrient content may be ameliorated by introducing specific microorganisms into the soil. This study aims to evaluate the effects of a highly efficient phosphate solubilizing bacterium MWP-1, Pseudomonas poae, on plant growth, soil nutrients in remedying the soil of the high-altitude Muli mining area in Qinghai Province, and analyze its impact on microbial communities through high-throughput sequencing soil microbial communities. The results showed that MWP-1 significantly increased the content of soil available phosphorus by >50%, soil organic matter and total nitrogen by >10%, and significantly increased the height, coverage, and aboveground biomass of vegetation by >40% in comparison with the control (p < 0.05). MWP-1 mainly affected the composition of the soil bacterial communities at the taxonomic level below the phylum. Its impact on soil fungal communities occurred at the phylum and below taxonomic levels. In addition, MWP-1 also significantly improved the diversity of soil bacterial and fungal communities (p < 0.05), and changed their functions. It also significantly altered the relative abundance of genes regulating phosphorus absorption and transport, inorganic phosphorus dissolution and organic phosphorus mineralization in the bacterial community (p < 0.05). It caused a significant increase in the relative abundance of the genes regulating nitrogen fixation and nitrification in nitrogen cycling (p < 0.05), but a significant decrease in the genes regulating phospholipase (p < 0.05). Although sequencing results indicated that Pseudomonas poae did not become the dominant species, its dissolved phosphorus elements can promote plant growth and development, enrich soil nutrient content, and affect the succession of microbial communities, enhance ecosystem stability, with an overall positive effect on soil remediation in the mining area.

The Impact of Transformational Leadership on Leaders’ Performance in the Context of Early Education Institutions

Early education leaders need to embrace transformational leadership styles that promote growth and achievement to contribute to the success of the entire community. Research in this area is necessary because, according to conversations with school inspectors, the leaders of early education institutions in Romania need an adequate understanding of leadership styles, as seen by the lack of professional development courses available. Our research aimed to assess the impact of transformational leadership style on early education leaders' success as measured by operational indicators such as effectiveness, satisfaction, and extra effort. In this study, we employed the Multifactor Leadership Questionnaire (MLQX5) and focus group method. To determine the dominating style of the leaders in the study, correlations, regressions, and the Chi-square test were used to analyze the data. The findings indicate a connection between transformative leadership and a growth mindset that characterizes leaders. These aspects lead to high effectiveness and stimulate the individual towards continuous personal and professional development. We can conclude from the investigation that the outcomes of the leader's behavior are closely related to their leadership style and growth mindset.

Microbial Community Structure, Diversity, and Succession During Decomposition of Kiwifruit Litters with Different Qualities

There are differences in the litter quality and decomposition rate of kiwifruit varieties, but it is not clear whether these differences are related to microbial communities. The leaf litters of two kiwifruit varieties (A. chinensis cv ‘Hongyang’ and A. chinensis cv ‘Jinyan’) were taken as objects, and the structure, diversity, and succession of the soil microbial communities were analyzed using an in situ decomposition experiment. Moreover, the contents of C, N, P, and K in the litters during decomposition were analyzed. The results show that there were variety differences in community structure at the generic level. Lophotrichus, Acaulium, and Fusarium were relatively more abundant in the microbial community of the ‘Hongyang’ kiwifruit litter, and Humicola and Tausonia were relatively more abundant in the microbial community of the ‘Jinyan’ kiwifruit litter. Subgroup_6 and Sphingomonas were the dominant bacteria. The bacterial community diversity of ‘Jinyan’ kiwifruit was higher than that of the ‘Hongyang’ kiwifruit litter. The community diversity was higher in the middle and later periods. The contents of C and N in the litters were the main factors affecting microbial communities. The abundances of Humicola and Apiotrichum were negatively correlated with the contents of C and N, and the abundances of Sphingomonas and SC-I-84 were positively correlated with the content of C. There were variety differences in the microbial communities corresponding to the decomposition processes of the ‘Hongyang’ and ‘Jinyan’ kiwifruit litters. The mechanisms of the variety differences were related to litter quality and the initial soil microbial community.

Bacterial community structure and metabolism response to antibiotic residue levels in pig manure anaerobic digestion

Bacterial community succession in pig manure anaerobic digestion and its correlation with antibiotic residues under combinations of digestion time, temperature, moisture, acidity, and alkalinity are unknown. In this study, bacterial communities and antibiotic residues in compost were analyzed under combinations of these influencing factors (digestion time, temperature, moisture, and acidity and alkalinity). The results showed a decrease in antibiotic residues in the digestion, including tetracycline, oxytetracycline, chlortetracycline, norfloxacin, ciprofloxacin, penicillin, cephalosporin, sulfamethoxypyrimidine, sulfamethazine, tylosin, and erythromycin. These combined conditions resulted in changes in the structure and composition of the dominant and rare bacterial communities. However, there were bacterial populations (containing 13 genera) that presented a stable composition. Antibiotic residue levels were significantly associated with the genus numbers of dominant bacterial communities (containing 29 genera), but not with rare and stable bacterial communities. Antibiotic residue levels were significantly negatively correlated with the abundance of microbial genes encoding carbohydrate-active enzymes, negatively correlated with the abundance of genes involved in main metabolic pathways, and positively correlated with the abundance of genes associated with resistance to environments. Our findings suggest insight into correlations between bacterial community structure and metabolism and antibiotic residues in pig manure anaerobic digestion under effects of key influencing factors.

How Ecological Filters Influence the Dynamics of Re-Built Plant Communities and Functional Composition in Open-Pit Mine over 14 Years

The traditional ecological reclamation measurements and assessments for the grassland areas damaged by open-pit mining often fall short in revealing the dynamics of plant communities affected by environmental filters during reconstruction, making reclamation efforts crucial. The trait-based community framework has been widely applied due to its great potential to predict the restoration process and provide insight into its mechanisms, but how the traits and environmental factors interact to form communities over time is still uncertain. Therefore, to make this process clear, we used the trait-based community framework, defining target species, non-target species, and common grass species, examining how the mix seed sowing and environment (two surface-covering materials applied to mine dump) affect re-vegetation composition, diversity, and functional traits in 14 years. Four treatments were tested: bio-fence surface-covering materials + sowing (BFS), plant-barrier surface-covering materials + sowing (PBS), sowing without any surface-covering materials (SOW), and a control without seeding and covering (CK). Natural grassland sites were regarded as reference (REF). Our findings indicated that the mix seed sowing and the interaction of surface-covering and time were primarily driving the dynamics of the plant community, affecting composition, the value of diversity, coverage, numbers, richness, and functional traits, such as the community-weighted mean (CWM) and functional diversity (FD), which increased and approached the sites REF. There were significant differences between the treatments and CK for the most traits. Although several results in the treatments approached the REF, significant differences still remained in the last observation year. With the sowing and surface-covering treatment, the re-built communities became more resource-acquisitive in terms of the CWM traits; even the value of the specific leaf area (SLA) exceed the REF after 14 years reclamation. We found those communities were dominated by target species that had a higher traits value than the non-target species, while the CK treatment became more resource-conservative over time due to non-target species dominating. The CWM in treatments tended toward reference levels for specific leaf area (SLA), leaf dry matter content (LDMC), and root dry matter content (RDMC), but not for seed mass (SM), thereby indicating that the above- and below-ground productivity of restored sites gradually overcame abiotic (surface-covering) and biotic (sowing) filters and approached target values. The functional diversity (FD) generally increased, with higher multivariate functional dispersion in the treatments containing more target species, suggesting that re-built communities achieve more resistance to invasion and disturbance over time. Hence, the trajectory of species and communities changing highlights the effectiveness of a trait-based approach in identifying better reclamation treatments and candidate species and provides a positive outlook for future re-vegetation community succession.

Building Sustainable Community: Insight from successful waste management initiative

The global waste situation presents a significant challenge worldwide, especially in developing countries experiencing rapid economic development and urban growth. Waste separation for recycling is a recognized sustainable management method that overcomes budget limitations and the lack of advanced technology. However, promoting waste separation remains a challenging issue that requires learning from successful lessons to advance this agenda. This study examines a successful waste management initiative started by a community in response to a significant waste issue. It's a case study of a low-income community near Bangkok, Thailand. The study uses the theory of change to understand the community's success and the sustainable development goals (SDGs) to evaluate the sustainability of the waste management efforts. The study highlights how the community effectively addressed a significant waste problem that was negatively impacting water sources and causing pollution. The leadership of the community leader played a crucial role in implementing successful strategies, including developing diverse participation approaches to meet the needs of various community members. The community utilized the PDCA (Plan Do Check Act) cycle to drive progress, and the task force operated on a foundation of equality, fostering horizontal relationships, and distribute of incentives. The process of effecting change was described in line with the theory of change, resulting in a successful outcome. Ultimately, this study will offer valuable insights for low-income communities worldwide, empowering them to tackle waste issues and foster sustainability within their neighborhoods.

Effectively increase the L(+)-isomer proportion of ethyl lactate in Baijiu by isolating and applying L(+)-lactic acid-producing bacteria

Lactic acid (LA) is a significant byproduct in Baijiu fermentation, with both isomers of LA and ethyl lactate (EL) being present in strong-aroma Baijiu. D(-)-isomers cannot be metabolized by humans, and their excessive accumulation can be harmful. This study aims to enhance the proportion of the beneficial L(+)-isomer in Baijiu. We analyzed 106 commercially available strong-aroma Baijiu samples, along with fermented grains (Zaopei), yellow water (Huangshui), and Baijiu samples from 10 Baijiu factories, confirming the coexistence of D(-)-LA and L(+)-LA in fermentation products. Notably, a strong correlation was found between L(+)-LA in Zaopei and L(+)-EL in Baijiu. Microbial community analysis revealed that Lactobacillus and Bacillus were the dominant bacterial groups, with a succession of microbial communities throughout fermentation. We isolated two strains from Zaopei with high selectivity for L(+)-LA production: Bacillus licheniformis (A6) and Bacillus cereus (B12). Incorporating these strains into both liquid- and solid-state fermentation systems markedly increased the proportion of L(+)-EL relative to total ethyl lactate in Baijiu. This study successfully isolated and applied L(+)-LA-producing strains to fermentation models, offering a practical strategy for increasing L(+)-LA content in Baijiu.

Study on the relationship between microbial community succession and physicochemical factors in the fermentation of rice-flavor Baijiu based on high-throughput and redundancy analysis techniques

Fermented grains are the main carrier of microbial fermentation and the direct source of Baijiu flavor substances, but so far, there are few studies on the changes in the fermented grains of rice-flavor Baijiu. This experiment systematically analyzed the correlation between microbial community succession and physicochemical factors in the fermentation process of two different types of rice (japonica: M and indica: H) fermented grains. The results showed that during the fermentation process, the changes in total acid, pH, starch, and total phenols of H and M were consistent. However, M and H showed different trends in terms of reducing sugar content and alcohol content. At the phylum level, both types of fermented grains had Firmicutes and Proteobacteria as the absolute dominant phyla. At the genus level, there was a slight difference in the dominant genera of M and H in the early stages, but the main genera in the later stages of fermentation were different. RDA analyses showed different correlations between dominant genera and physicochemical factors with some interesting results. The results of this study will provide a theoretical basis for the regulation of rice-flavor Baijiu fermentation.

A Novel Behavioral Intervention to Enhance Physical Activity for Older Veterans in a Skilled Nursing Facility.

Physical activity levels during skilled nursing facility (SNF) rehabilitation fall far below what is needed for successful community living and to prevent adverse events. This feasibility study's purpose was to evaluate the feasibility, acceptability, and preliminary effectiveness of an intervention designed to improve physical activity in patients admitted to SNFs for short-term rehabilitation. High-Intensity Rehabilitation plus Mobility combined a high-intensity (i.e.,high weight, low repetition), progressive (increasing in difficulty over time), and functional resistance rehabilitation intervention with a behavioral economics-based physical activity program. The behavioral economics component included five mobility sessions/week with structured goal setting, gamification, and loss aversion (the idea that people are more likely to change a behavior in response to a potential loss over a potential gain). SNF physical therapists, occupational therapists, and a mobility coach implemented the High-Intensity Rehabilitation plus Mobility protocol with older Veterans (n = 18) from a single SNF. Participants demonstrated high adherence to the mobility protocol and were highly satisfied with their rehabilitation. Treatment fidelity scores for clinicians were ≥95%. We did not observe a hypothesized 40% improvement in step counts or time spent upright. However, High-Intensity Rehabilitation plus Mobility participants made clinically important improvements in short physical performance battery scores and gait speed from admission to discharge that were qualitatively similar to or slightly higher than historical cohorts from the same SNF that had received usual care or high-intensity rehabilitation alone. These results suggest a structured physical activity program can be feasibly combined with high-intensity rehabilitation for SNF residents following a hospital stay.

Bacterial and fungal diversity, community composition, functional groups, and co-occurrence network succession in dryland and paddy soils along a 3000-year chronosequence

There is a limited understanding of soil microbial community successional trajectories during dryland and paddy soil long-term management after wetland reclamation. We examined the effects of long-term reclamation on the microbial diversity, community composition, functional groups, and co-occurrence network in dryland and paddy soils using a 3000-year soil chronosequence established in the floodplain of the middle and lower Yangtze River, employing the “space-for-time” method and 16S rRNA and ITS amplicon sequencing. Over time, reclaimed land experienced varying losses in microbial richness and diversity, with paddy soils exhibiting higher alpha diversity than drylands due to greater resources and ecological niches. Proteobacteria dominated drylands, Chloroflexi prevailed in paddy soils, and fungal communities in both land-use types were primarily Ascomycota and Basidiomycota. Functional predictions showed higher chemoheterotrophy and aerobic_chemoheterotrophy in dryland soils and more animal_parasites_or_symbionts and dung_saprotrophs in paddy soils. During the 2000–3000 year reclamation, the abundance of pathogens increased, posing risks to agricultural production. Co-occurrence network analysis indicated that, compared to paddy soils, dryland soils may form a more complex and stable microbial cooperative network. Soil organic carbon and pH influenced microbial communities in drylands, while bulk density and cation exchange capacity were crucial in paddy soils. Partial least squares path modeling indicated that reclamation duration impacts microbial network complexity and diversity, with soil properties regulating paddy soil microbial networks. These findings broaden our understanding of microbial community succession in dryland and paddy soils after long-term wetland reclamation, supporting soil health and sustainable agriculture.

Active restoration efforts drive community succession and assembly in a desert during the past 53 years.

Regreening efforts in deserts have been implemented globally to combat land degradation and desert expansion, but how they affect above- and belowground community succession and assembly processes remains unknown. Here, we examined variations in plant and soil microbial community attributes along a 53-year restoration chronosequence following the establishment of straw checkerboard barriers (SCBs) in the Tengger Desert of China. This approach is a combination of fixing shifting sand and adding organic material (straw) simultaneously to expedite vegetation restoration by enhancing the success of plant establishment. Our findings revealed that the establishment of SCBs significantly triggered plant and soil microbial communities to gradually approximate those of the natural community along restoration duration. We observed positive and negative bidirectional shifts in plant and soil microbial community composition. Critical temporal threshold zones for relatively rapid changes in community composition were identified, with 2-15.5 years for plants, 0.5-8.5 years for bacteria, and 2-8.5 years for fungi. This suggests a delayed response of plant communities to restoration efforts compared with soil microbial communities. Both stochastic and deterministic processes regulated plant and soil microbial community assembly. Stochastic processes played a more important role in plant and fungal community succession, whereas deterministic processes primarily governed bacterial succession. In terms of deterministic processes, temporal variations in community composition mainly resulted from the intrinsic correlations among plant, bacterial, and fungal communities, as well as an increase in soil organic carbon (SOC) with restoration duration. Thus, temporal patterns and functional contributions of bacterial communities appear to be more predictable than those of plant and fungal communities during desert ecosystem restoration. This study emphasizes that plant-bacteria-fungi correlations and increasing SOC content are critical for accelerating community succession and promoting dryland restoration. Future studies should explore and integrate temporal variations and restoration effects of multiple ecosystem functions to better predict dryland development and resilience to global climate changes over a large temporal scale.

Seasonality shapes the microbiota and metabolome of strong-flavor Baijiu during fermentation and affects its flavor characteristics

The microbial community and product flavor of fermented foods are subject to seasonal environmental fluctuations due to open production environment. However, research on the impact of external environmental changes caused by seasonal shifts on brewing is limited. Here, we studied changes in physicochemical indicators and flavor compounds of fermented grains during Baijiu fermentation in four seasons, as well as the succession and assembly of microbial communities. The temperature throughout fermentation was highest in summer, followed by spring and autumn, and lowest in winter. Winter had slower rates of moisture growth, total acidity accumulation, and sugar consumption than the other seasons. At the end of fermentation, the highest total organic acid content was identified in summer, and the lowest level of volatile compounds was found in winter. We found significant differences in microbial community structure across different seasons. Meanwhile, compared to fungal community, the succession of bacterial community was more susceptible to brewing environment, with reducing sugar being the main driving factor in the early stage and total acidity, organic acids, and moisture being the main driving factors in the late stage. The microbial co-occurrence networks in autumn and winter were more complex than those in spring and summer. Furthermore, brewing in the spring and summer promoted the accumulation of acids in Baijiu, esters in summer, ethyl esters in autumn, and aldehydes and ketones in winter. This study offers a fresh perspective on the microbial brewing mechanism of Baijiu by exploring the seasonal variations of microbiota and metabolome.

Mediterranean co-living: succession of soil mycorrhizal communities associated with Halimium lasianthum shrubs

Mediterranean co-living: succession of soil mycorrhizal communities associated with Halimium lasianthum shrubs

Dispatched microbial community assembly processes driving ecological succession during phytostabilization of mercury-rich tailings

Phytostabilization is an important way for the remediation of mine tailings, but the associated microbial processes and community succession remain largely unknown. In this study, we investigated the assembly mechanisms maintaining the core and satellite subcommunities diversity during phytostabilizaion of a mercury-rich mine tailings. The contents of total Hg and methylmercury decreased with a concomitant increase of total and available phosphorus content along the successive remediation stages. Microbial community composition, profiled by 16S rRNA gene sequencing, revealed amplicon sequence variants (ASVs) that were separated according to their abundance within either the core community or the satellite community. Community dynamics analysis showed that alpha diversity indices increased for the core community while decreased for the satellite community. Both satellite and core communities were mainly driven by stochastic drift process, and homogeneous selection was relatively higher in shaping the core community organization. The core community included ASVs affiliated to Proteobacteria, Crenarchaeota, Bacteroidota, Verrucomicrobiota, Acidobacteriota, and Myxococcota phyla, which were driven primarily by heterogeneous selection and drift. The satellite community included ASVs affiliated to Acidobacteriota, Ktedonobacteria, Anaerolineae and Verrucomicrobiota phyla, which were mainly influenced by heterogeneous selection. Nineteen taxa and one taxon were identified as keystone taxa for the satellite and core communities respectively. This study provides important insights on the assemble rules within the core and satellite communities, and theoretical guidance for further ecological restoration and management during microbial remediation of metal-mined derelict land.