Subtropical Region Of Southern China Research Articles

Effects of changes in precipitation patterns and stream flow on stream DOC and [formula omitted] concentrations in a headwater catchment in a subtropical region of southern China

Study regionThe Guantian headwater watershed is located in the subtropical region of South China. Study focusThis study examined how changes in precipitation and consequent shifts in streamflow affect dissolved organic carbon (DOC) and nitrate (NO3−) concentrations in a headwater stream. New hydrological insights for the regionThe results indicate that stream DOC and NO3− concentrations decrease from spring to winter (0.16 mg/L/month for DOC and 0.18 mg/L/month for NO3−) and have a generally positive relationship with streamflow (p < 0.05). Changes in precipitation-concentration, precipitation-amount and consequent shifts in streamflow remarkably affect stream DOC and NO3− concentrations. When precipitation events are more heavily concentrated (when precipitation concentration index (PCI) changed from 12.81 to 18.33) from May to July, the stream DOC and NO3− concentrations tend to increase from May to July but decrease before and after this period. In addition, an increase in precipitation-amount tends to increase stream DOC and NO3− concentrations before the occurrence of peak flow and in the dry period from September to December, but decreases them in the wet period from June to September. When the precipitation-amount decreases, the trend of the change in concentration is exactly the opposite. These findings suggest that stream DOC and NO3− concentrations in the headwater stream are highly chemodynamic: they are transport-limited before the occurrence of high flow and in the dry period from September to December, while they are supply-limited in the June-September period.

Aquatic photosynthetic carbon pump driven by periodic temperature variations affects dissolved inorganic carbon and hydrochemical characteristics in a groundwater-fed reservoir

Under the influence of periodic temperature variations, biogeochemical cycling in water bodies is markedly affected by the periodic thermal stratification processes in subtropical reservoirs or lakes. In current studies, there is insufficient research on the influence and mechanism of dissolved inorganic carbon (DIC) distribution in karst carbon-rich groundwater-fed reservoirs under the coupled effects of thermal structure stratification and the biological carbon pump (BCP) effect. To address this issue, the Dalongdong (DLD) reservoir in the subtropical region of southern China was chosen as the site for long-term monitoring and research on relevant physicochemical parameters of water, DIC, and its stable carbon isotope (δ13CDIC), CO2 emission flux, as well as the reservoir's thermal stratification index. The results show that: (1) the DLD reservoir is a typical warm monomictic reservoir, which exhibits regular variations of mixing period-stratification period-mixing period on a yearly scale due to thermal structure changes; (2) DIC was consumed by aquatic photosynthetic organisms in the epilimnion during the stratification period, leading to a decrease in DIC concentration, partial pressure of CO2 (pCO2) and CO2 emission flux, and an increase in stable carbon isotope (δ13CDIC). During the mixing period, the trend was reversed; (3) During the thermal stratification, aquatic photosynthesis and water temperature were the primary factors controlling DIC variations in both the epilimnion and thermocline. Regarding the hypolimnion, calcite dissolution, organic matter decomposition, and water temperature were the dominant controlling factors. These results indicate that although carbon-rich karst groundwater provides a plentiful supply of DIC in the DLD reservoir, its availability is still influenced by variations in the reservoir's thermal structure and the metabolic processes of aquatic photosynthetic organisms. Therefore, to better estimate the regional carbon budget in a reservoir or lake, future studies should especially consider the combined effects of BCP and thermal structure variations on carbon variations.

Mapping Insect-Proof Screened Citrus Orchards Using Sentinel-2 MSl Time-Series Images

Citrus is a crucial agricultural commodity of the hilly subtropical regions of southern China. Attempts in recent years to combat the destructive disease Huanglongbing (HLB) have led to citrus orchards being covered with insect-proof screens (IPS). Understanding which citrus orchards are covered by IPS is crucial for regional water and soil conservation, as well as control of plastic pollution. However, monitoring of orchards is complicated by IPS spectral interference in remotely sensed image classification. Here, an optimal feature combination scheme is developed and tested for mapping citrus orchards that use IPS. Seasonal Sentinel-2 images from 2021 were used to define indices for vegetation, plastic mulch, red edge, and texture. These were combined with topographic and land surface temperature using random forest classification to determine optimal feature discrimination combinations for orchards in Xunwu County, Jiangxi Province. Results show: (1) significantly higher visible light reflectance from IPS orchards ensures spectral discrimination between IPS covered and uncovered orchards. (2) After feature optimization, the seasonal spectral band has the highest accuracy (86%) in single feature classification. The addition of conventional indices and topographic-temperature features improves classification to 92%. (3) Xunwu County had 460 km2 of citrus orchard cover in 2021, with 88 km2 (19%) of that total being covered with IPS. Our method effectively and accurately maps citrus orchards with or without IPS coverage at 10 m resolution. The effective monitoring of large-scale IPS in other regions can now support the development of local and regional sustainable agricultural policies.

A Novel 5 kb Deletion in the β-Globin Gene Cluster Identified in a Chinese Patient

β-Thalassemia (β-thal), a highly prevalent disease in tropical and subtropical regions of Southern China, is caused mainly by point mutations in the β-globin gene cluster. However, large deletions have also been found to contribute to some types of β-thal. We identified a novel 5 kb deletion in the β-globin cluster in a Chinese patient using multiplex ligation-dependent probe amplification (MLPA), and characterized it with single molecule real-time (SMRT) sequencing, gap-polymerase chain reaction (gap-PCR) and Sanger sequencing. The deletion was located between positions 5226189 and 5231091 on chromosome 11 (GRCh38), extending from 4 kb upstream of the 5' untranslated region (5'UTR) to the second intron of the β-globin gene. The patient with this deletion presented with microcytosis and hypochromic red cells, as well as relatively high Hb F and Hb A2 levels. Our research indicated that SMRT sequencing is a useful tool for accurate detection of large deletions. Our study broadens the spectrum of deletional β-thalassemias and provides a perspective for further study of the function of the β-globin cluster.

Comparison of feature selection methods for mapping soil organic matter in subtropical restored forests

• Filter, wrapper, embedded and ensemble feature selection approaches (FS) were evaluated. • The proposed ensemble FS incorporates ten individual selectors to identify top significant variables. • Ensemble FS produced the highest predictive variable subset for mapping SOM. • XGBoost outperformed RF in mapping SOM in subtropical restored forests. Mapping Soil organic matter (SOM) over a complex forest landscape is challenging due to the difficulty in selecting the most insightful variables from high-dimensional datasets in the recent explosion of geospatial-data. Feature selection (FS) is necessary to reduce data redundancy and noise as well as to achieve more reliable SOM spatial predictions. However, it is still unclear that which is most effective among various FS methods in mapping SOM. Therefore, four types of FS approaches (i.e., filter, wrapper, embedded and ensemble) were adopted to generate optimum variable subsets from an original variable dataset of 60 candidates, respectively, for mapping SOM of restored forest land in a typical subtropical region of southern China. The most used methods for each type of FS approaches were selected in this study, including three filters (Chi-square, InfoGain and pearson correlation analysis), three wrappers (genetic algorithm, simulated annealing algorithm and support vector machine-recursive feature elimination) and three embedded methods (Boruta, random forest (RF) and extreme gradient boosting (XGBoost)), as well as an ensemble method (robust rank aggreg algorithm (RRA)). Meanwhile, the RF and XGBoost models were applied with a 10-fold cross-validation method to compare the relative advantages of the different FS methods in SOM mapping, by utilizing the correlation coefficients R 2 between observed and predicted values and predicting errors of root mean square error (RMSE). The results show that the SOM prediction accuracies with optimized variable subsets generated by the different FS methods are better than those with full variables, yet the improvements of prediction performance are different among the four types of FS approaches. The ensemble method (RRA) is superior to the other three types of approaches with an average RMSE reduction of 9.16% comparing to that without using FS methods, followed by wrapper and embedded methods which obtained the average RMSE reduction by 7.81%, 7.32%, respectively, and the filter methods are the weakest in the RMSE reduction with slight decreases of 4.32%. The XGBoost model achieved a better performance in predicting SOM than the RF model regardless of input variables, and the XGBoost model combined with RRA FS method shows the greatest potential to map SOM in the restored forest land. This study provides a reference for obtaining more parsimonious and robust variable sets from the available big geo-data freely for soil mapping in other areas.

Effects of Biochar Feedstock and Pyrolysis Temperature on Soil Organic Matter Mineralization and Microbial Community Structures of Forest Soils

Biochar has received much attention as a strategy to enhance soil carbon (C) sequestration and mitigate climate change. Previous studies found that the feedstock and pyrolysis temperature can largely determine biochar properties, which in turn, impact the stability of native soil organic matter (SOM) and soil microorganisms. The Schima superba and Cunninghamia lanceolata are two tree species widely distributed in the subtropical region of southern China, but how the biochars from these two species influence the soil C sequestration and microbial communities of plantation remain poorly understood. In this study, we produced biochars from these two different feedstocks (13C-labeled S. superba and C. lanceolata litters) at three pyrolysis temperatures (350°C, 550°C, 750°C), then added them to the soils from C. lanceolata plantation, and maintained the experiments at 25°C for 112 days. We found both C mineralization and soil microbial community structures were strongly, but inconsistent, affected by biochar feedstock and pyrolysis temperature. The C. lanceolata biochar triggered the negative priming effect faster and greater compared with the S. superba biochar amendment. Biochars produced at 550°C showed the most significant negative priming effect during the whole incubation period, regardless of the different feedstocks. The cumulative amount of CO2 derived from biochars was significantly decreased with pyrolysis temperature (p &amp;lt; 0.05), indicating that biochars prepared at higher temperatures were more stable in the soil. Further, the soil microbial community structure was only affected by biochar pyrolysis temperature rather than biochar feedstock and their interaction. Together, our results reveal that biochar feedstock and pyrolysis temperature may play more important roles in dictating the priming effect than the structure of microbial community for C. lanceolata plantation. Overall, we concluded that the biochars prepared at 550°C could rapidly decrease the turnover of native SOM in a short term and biochar amendment has the potential to be a management practice for soil C sequestration in the C. lanceolata plantation.

Identifying optimal ridge practices under different rainfall types on runoff and soil loss from sloping farmland in a humid subtropical region of Southern China

The relationship between different rainfall types, different soil management practices, and soil erosion is not yet fully understood. In-situ observations of soil and water loss on sloping farmland in the red soil region of southern China with a subtropical monsoon environment were taken at 12 runoff plots with four treatments, i.e, downslope ridges, downslope ridges with hedgerow intercropping, contour ridges, and bare flat land as control, over a seven-year period from 2012 to 2018. During this time, 253 natural rainfall events were classified into three rainfall types by K-means clustering according to the rainfall depth, maximum-30 min rainfall intensity and rainfall duration, and surface runoff and soil erosion processes in relation to the rainfall types under different ridge practices were analyzed. The results show that water-induced soil erosion on the flat land control was significant, with average annual soil loss of 76.73 t·ha−1·yr−1, reaching the “intense erosion” classification, and ridge practices were confirmed to reduce annual runoff and soil loss in all rainfall events by 18.9–62.0% and 68.9–86.3%, respectively. On the whole, rainfall events can be divided into three types: intense, normal, and long-duration. Among them, intense and normal rainfall cause the majority of soil (88.5–93.7%) and water (75.0–83.8%) loss in this area, but the efficiencies in runoff and soil reduction during long-duration rainfall events were the lowest, or even negative on farmlands with only downslope ridges. 20% of the total rainfall events, in which 84.3–92.2% were intense and normal rainfall events, contributed to 29–33% of the total rainfall depth, 68–89% of the total runoff depth, and 94–98% of the total soil loss. Rainfall depth played a dominant role in generating runoff, while runoff accumulation was a main factor influencing on soil loss. Findings from our study indicate that by choosing a more appropriate ridge practice according to different rainfall types, there can be a positive effect on soil and water conservation.

Short-Term Temperature Response of Leaf Respiration in Different Subtropical Urban Tree Species.

Plant leaf respiration is one of the critical components of the carbon cycle in terrestrial ecosystems. To predict changes of carbon emissions from leaves to the atmosphere under a warming climate, it is, therefore, important to understand the thermodynamics of the temperature response of leaf respiration. In this study, we measured the short-term temperature response of leaf respiration from five different urban tree species in a subtropical region of southern China. We applied two models, including an empirical model (the Kavanau model) and a mechanistic model (Macromolecular Rate Theory, MMRT), to investigate the thermodynamic properties in different plant species. Both models are equivalent in fitting measurements of the temperature response of leaf respiration with no significant difference (p = 0.67) in model efficiency, while MMRT provides an easy way to determine the thermodynamic properties, i.e., enthalpy, entropy, and Gibbs free energy of activation, for plant respiration. We found a conserved temperature response in the five studied plant species, showing no difference in thermodynamic properties and the relative temperature sensitivity for different species at low temperatures (<42°C). However, divergent temperature response among species happened at high temperatures over 42°C, showing more than two-fold differences in relative respiration rate compared to that below 42°C, although the causes of the divergent temperature response remain unclear. Notably, the convergent temperature response at low temperatures could provide useful information for land surface models to improve predictions of climate change effects on plant respiration.

Effect of Different Rates of Nitrogen Fertilization on Crop Yield, Soil Properties and Leaf Physiological Attributes in Banana Under Subtropical Regions of China.

Excessive nitrogen (N) application is widespread in Southern China. The effects of N fertilization on soil properties and crop physiology are poorly understood in tropical red loam soil. We conducted a field experiment to evaluate the effect of nitrogen fertilization rates on physiological attributes (chlorophyll, plant metabolic enzymes, soluble matters) on banana leaves, soil properties (soil enzymes, soil organic matter (SOM), soil available nutrients) as well as banana crop yield in a subtropical region of southern China. The N rates tested were 0 (N0), 145 (N145), 248 (N248), 352 (N352), 414 (NFT), and 455 (N455) g N per plant. The correlations among soil factors, leaf physiological factors and crop yield were evaluated. The results indiated that the high rates of N fertilization (NFT and N455) significantly decreased soil available potassium (K) content, available phosphorus (P) content, glutamine synthetase (GS) activity, and soluble protein and sugar contents compared with lower N rates. The N352 treatment had the highest crop yields compared with higher N rates treatments, followed by the N455 treatment. However, there were no significant differences in crop yields among N fertilization treatments. Factor analysis showed that the N352 treatment had the highest integrated score for soil and leaf physiological factors among all treatments. Moreover, the N352 treatment was the most effective in improving carbon and nitrogen metabolism in banana. Crop yield was significantly and positively linearly correlated with the integrated score (r = 0.823, p < 0.05). Path analysis revealed that invertase, SOM and sucrose synthase (SS) had a strong positive effect on banana yield. Canonical correspondence analysis (CCA) suggested that available K, invertase, acid phosphatase and available P were the most important factors impacting leaf physiological attributes. Cluster analysis demonstrated distinct differences in N application treatment related to variations in soil and leaf factors. This study suggested that excessive N fertilization had a negative effect on soil fertility, crop physiology and yield. The lower N rates were more effective in improving crop yield than higher rates of N fertilization. The N rate of 352 g N per plant (N352) was recommended to reduce excess N input while maintaining the higher yield for local farmers’ banana planting.

Paramyxoviruses respiratory syncytial virus, parainfluenza virus, and human metapneumovirus infection in pediatric hospitalized patients and climate correlation in a subtropical region of southern China: a 7-year survey

To investigate the features of paramyxovirus respiratory syncytial virus (RSV), parainfluenza virus (PIV), and human metapneumovirus (HMPV) infection and determine the effect of meteorological conditions in Guangzhou, a subtropical region of southern China. We collected 11,398 respiratory samples from hospitalized pediatric patients with acute respiratory illness between July 2009 and June 2016 in Guangzhou. The samples were tested simultaneously for 18 respiratory pathogens using real-time PCR. Local meteorological data were also collected for correlation analysis. Of 11,398 patients tested, 5606 (49.2%) patients tested positive for one or more pathogens; RSV, PIV, and HMPV were the first, sixth, and ninth most frequently detected pathogens, in 1690 (14.8%), 502 (4.4%), and 321 (2.8%) patients, respectively. A total 17.9% (4605/5606) of patients with positive results had coinfection with other pathogens. Significant differences were found in the prevalence of RSV, PIV, and HMPV among all age groups (p < 0.001). RSV and HMPV had similar seasonal patterns, with two prevalence peaks every year. PIV appeared alternatively with RSV and HMPV. Multiple linear regression models were established for RSV, PIV, and HMPV prevalence and meteorological factors (p < 0.05). RSV and PIV incidence was negatively correlated with monthly mean relative humidity; RSV and HMPV incidence was negatively correlated with sunshine duration; PIV incidence was positively correlated with mean temperature. We described the features of paramyxovirus infection in a subtropical region of China and highlighted the correlation with meteorological factors. These findings will assist public health authorities and clinicians in improving strategies for controlling paramyxovirus infection.

Manure acts as a better fertilizer for increasing crop yields than synthetic fertilizer does by improving soil fertility

Fertilization is an important management strategy for crop yields by mediating soil fertility. However, rare studies quantitatively assessed the interactions among fertilization, crop yields, and soil fertility. Here, data from a 25-year fertilization experiment in the humid subtropical region of Southern China were used to evaluate and quantify the effect of fertilization on crop yields via soil fertility. Seven treatments were chosen: CK (non-fertilizer); N (synthetic nitrogen); NP (synthetic N and phosphorus); NPK (synthetic N, P and potassium); NPKM1 (synthetic NPK with manure); 1.5NPKM1 (1.5 times of NPKM1); and M2 (manure alone). Overall, the crop yields of wheat and maize under manure (1.36–1.58 and 3.85-5.82 Mg ha−1) were higher than those under CK (0.34 and 0.25 Mg ha−1) and synthetic fertilized treatments (0.27–0.97 and 0.48–2.65 Mg ha−1), as the averaged of 1991–2015. Higher SOC stocks were found under the NPKM1, 1.5NPKM1, and M2 treatments with a pronounced increase in SOC over the first 10 years and stable over the last 15 years. By the boosted regression trees, manure, synthetic fertilizer and soil properties (SOC storage, soil pH, and soil nutrients) accounted for 39%, 21%, and 40% of the variation of the relative yield, respectively. Path analysis identified a network of inter-relations of manure, synthetic fertilizer, and soil properties in the relative yields. Compared to synthetic fertilized treatments, manure application strongly and positively affected the relative yield by increasing SOC storage, soil nutrients, and soil pH (path coefficients: 0.90, 0.88, and 0.76). These factors explained 72% of the crop yields' variance. These results suggest that manure application is a viable strategy for regulating crop yields due to its improvement in soil fertility.

Phenology-dependent variation in the non-structural carbohydrates of broadleaf evergreen species plays an important role in determining tolerance to defoliation (or herbivory)

Two broadleaf evergreen canopy species (Schima superba and Engelhardia roxburghiana) with different phenologies in a subtropical region of southern China were used to determine the influence of leaf phenology on the impact of an insect pest attack. S. superba regenerates its leaves in February, while E. roxburghiana regenerates its leaves in May. The moth Thalassodes quadraria attacked the two broadleaf evergreen species in March to April, and the newly produced leaves were removed for S. superba but not for E. roxburghiana. The young trees were artificially defoliated to imitate an insect pest attack during March 2014. Nonstructural carbohydrate (NSC) and growth measurements and a retrospective analysis based on the radial growth of mature trees were conducted in January 2015. The results showed that NSC concentrations decreased in S. superba during canopy rebuilding, and the subsequent defoliation severely inhibited leaf and shoot growth, prevented NSC restoration in roots and stem xylem, and caused high mortality. The insect outbreaks reduced the radial growth of S. superba. In contrast, E. roxburghiana experienced less growth retardation, lower mortality, and normal radial growth. Thus, taking phenology-dependent variation in NSCs into consideration, defoliation and insect pest outbreaks more negatively impacted S. superba than E. roxburghiana.

Determination of critical pH and Al concentration of acidic Ultisols for wheat and canola crops

Abstract. Soil acidity has become a principal constraint in dry land crop production systems of acidic Ultisols in tropical and subtropical regions of southern China, where winter wheat and canola are cultivated as important rotational crops. There is little information on the determination of critical soil pH as well as aluminium (Al) concentration for wheat and canola crops. The objective of this study is to determine the critical soil pH and exchangeable aluminium concentration (AlKCl) for wheat and canola production. Two pot cultures with two Ultisols from Hunan and Anhui (SE China) were conducted for wheat and canola crops in a controlled growth chamber. Aluminium sulfate (Al2(SO4)3) and hydrated lime (Ca(OH)2) were used to obtain the target soil pH levels from 3.7 (Hunan) and 3.97 (Anhui) to 6.5. Plant height, shoot dry weight, root dry weight, and chlorophyll content (SPAD value) of wheat and canola were adversely affected by soil acidity in both locations. The critical soil pH and AlKCl of the Ultisol from Hunan for wheat were 5.29 and 0.56 cmol kg−1, respectively. At Anhui, the threshold soil pH and AlKCl for wheat were 4.66 and 1.72 cmol kg−1, respectively. On the other hand, the critical soil pH for canola was 5.65 and 4.87 for the Ultisols from Hunan and Anhui, respectively. The critical soil exchangeable Al for canola cannot be determined from the experiment of this study. The results suggested that the critical soil pH and AlKCl varied between different locations for the same variety of crop, due to the different soil types and their other soil chemical properties. The critical soil pH for canola was higher than that for wheat for both Ultisols, and thus canola was more sensitive to soil acidity. Therefore, we recommend that liming should be undertaken to increase soil pH if it falls below these critical soil pH levels for wheat and canola production.

Different responses of influenza epidemic to weather factors among Shanghai, Hong Kong, and British Columbia.

Weather factors have long been considered as key sources for regional heterogeneity of influenza seasonal patterns. As influenza peaks coincide with both high and low temperature in subtropical cities, weather factors may nonlinearly or interactively affect influenza activity. This study aims to assess the nonlinear and interactive effects of weather factors with influenza activity and compare the responses of influenza epidemic to weather factors in two subtropical regions of southern China (Shanghai and Hong Kong) and one temperate province of Canada (British Columbia). Weekly data on influenza activity and weather factors (i.e., mean temperature and relative humidity (RH)) were obtained from pertinent government departments for the three regions. Absolute humidity (AH) was measured by vapor pressure (VP), which could be converted from temperature and RH. Generalized additive models were used to assess the exposure-response relationship between weather factors and influenza virus activity. Interactions of weather factors were further assessed by bivariate response models and stratification analyses. The exposure-response curves of temperature and VP, but not RH, were consistent among three regions/cities. Bivariate response model revealed a significant interactive effect between temperature (or VP) and RH (P<0.05). Influenza peaked at low temperature or high temperature with high RH. Temperature and VP are important weather factors in developing a universal model to explain seasonal outbreaks of influenza. However, further research is needed to assess the association between weather factors and influenza activity in a wider context of social and environmental conditions.

Genomic Characterization of Travel-Associated Dengue Viruses Isolated from the Entry-Exit Ports in Fujian Province, China, 2013-2015.

Over the past decade, indigenous dengue outbreaks have occurred occasionally in Fujian province in southeastern China because of sporadic imported dengue viruses (DENV). In this study, 3 DENV-2 and 2 DENV-4 strains were isolated from suspected febrile travelers at 2 ports of entry in Fujian between 2013-2015. Complete viral genome sequences of these new isolates were obtained with Sanger chemistry. Genomic sequence analyses revealed that these strains belonged to genotypes of 2-Cosmopolitan and 4-II. Consistent with the patients' travel information, phylogenetic analyses of the complete coding regions also indicated that most of the new isolates were genetically similar to the circulating strains in Southeast Asia rather than previous Chinese strains that were available. Therefore, phylogenetic analyses of the imported DENV demonstrated that multiple introductions of DENV emerged continuously in Fujian, and highlighted the importance of dengue surveillance at entry-exit ports in the subtropical regions of southern China.

Evaluation of Eucalyptus red gum species and provenances for inland humid subtropical regions of southern China

ABSTRACTThere is a long history of eucalypt planting in inland regions of southern China where humid summers and frequent winter frosts and extreme cold events are features of the climate. Although early eucalypt plantings suffered widespread failures, some species managed to survive with individual trees in some plantings growing to impressive sizes with diameter at breast height (DBH) exceeding 50 cm. One of the most productive and best adapted species in the plantings was Eucalyptus camaldulensis. Two species–provenance trials were established in southern and central Hunan province in 2006 primarily to explore the potential of E. camaldulensis and the related E. tereticornis, but also to provide comparisons with two other species with potential for the climate. The trials included 65 provenances representing six taxa from four species: E. camaldulensis, E. tereticornis, E. amplifolia and E. macarthurii. Three provenances represented improved seed sources from Africa, whilst the others were natural stand provenances. Eucalyptus amplifolia var. amplifolia demonstrated the best potential for growth and survival up to age 5 years. Eucalyptus macarthurii had the best cold tolerance, but relatively poor performance for growth and survival. Both E. tereticornis and E. camaldulensis demonstrated reasonable cold tolerance, but growth and survival of E. camaldulensis var. camaldulensis provenances from temperate to subtropical climatic areas with dry summers and winter-dominant rainfall (Köppen climate classification Csa) were markedly inferior than those of E. tereticornis ig. ‘tereticamal’ and E. tereticornis ssp. tereticornis sourced from tropical and subtropical environments with summer-dominant rainfall (Köppen climate classifications Aw and Am). Seedlots of E. camaldulensis, E. tereticornis and E. macarthurii from improvement programs in Zimbabwe and South Africa mostly proved inferior to or no better than the best natural stand provenances of the same species in the Hunan environment.

Responses of absolute and specific soil enzyme activities to long term additions of organic and mineral fertilizer

Long-term phosphorus (P) and nitrogen (N) applications may seriously affect soil microbial activity. A long-term field fertilizer application trial was established on reddish paddy soils in the subtropical region of southern China in 1998. We assessed the effects of swine manure and seven different rates or ratios of NPK fertilizer treatments on (1) the absolute and specific enzyme activities per unit of soil organic carbon (SOC) or microbial biomass carbon (MBC) involved in C, N, and P transformations and (2) their relationships with soil environmental factors and soil microbial community structures. The results showed that manure applications led to increases in the absolute and specific activities of soil β-1,4-glucosidase(βG), β-1,4-N-acetylglucosaminidase (NAG), and leucine aminopeptidase (LAP). The absolute and specific acid phosphatase (AP) activities decreased as mineral P fertilizer application rates and ratios increased. Redundancy analysis (RDA) showed that there were negative correlations between absolute and specific AP activities, pH, and total P contents, while there were positive correlations between soil absolute and specific βG, NAG, and LAP enzyme activities, and SOC and total N contents. RDA showed that the contents of actinomycete and Gram-positive bacterium PLFA biomarkers are more closely related to the absolute and specific enzyme activities than the other PLFA biomarkers (P<0.01). Our results suggest that both the absolute and specific enzyme activities could be used as sensitive soil quality indicators that provide useful linkages with the microbial community structures and environmental factors. To maintain microbial activity and to minimize environmental impacts, P should be applied as a combination of inorganic and organic forms, and total P fertilizer application rates to subtropical paddy soils should not exceed 44kgPha−1year−1.

Red soils harbor diverse culturable actinomycetes that are promising sources of novel secondary metabolites.

Red soils, which are widely distributed in tropical and subtropical regions of southern China, are characterized by low organic carbon, high content of iron oxides, and acidity and, hence, are likely to be ideal habitats for acidophilic actinomycetes. However, the diversity and biosynthetic potential of actinomycetes in such habitats are underexplored. Here, a total of 600 actinomycete strains were isolated from red soils collected in Jiangxi Province in southeast China. 16S rRNA gene sequence analysis revealed a high diversity of the isolates, which were distributed into 26 genera, 10 families, and 7 orders within the class Actinobacteria; these taxa contained at least 49 phylotypes that are likely to represent new species within 15 genera. The isolates showed good physiological potentials for biosynthesis and biocontrol. Chemical screening of 107 semirandomly selected isolates spanning 20 genera revealed the presence of at least 193 secondary metabolites from 52 isolates, of which 125 compounds from 39 isolates of 12 genera were putatively novel. Macrolides, polyethers, diketopiperazines, and siderophores accounted for most of the known compounds. The structures of six novel compounds were elucidated, two of which had a unique skeleton and represented characteristic secondary metabolites of a putative novel Streptomyces phylotype. These results demonstrate that red soils are rich reservoirs for diverse culturable actinomycetes, notably members of the families Streptomycetaceae, Pseudonocardiaceae, and Streptosporangiaceae, with the capacity to synthesize novel bioactive compounds.

Silica‐scaled chrysophytes from Hainan, Guangdong Provinces and Hong Kong Special Administrative Region, China

Twenty five phytoplankton samples containing chrysophytes were collected in Hainan and Guangdong Provinces and in Hong Kong Special Administrative Region in January 2010 and October to November 2011. Forty nine taxa of silica‐scaled chrysophytes were identified by means of transmission electron microscopy, twelve of them belonging to Paraphysomonadaceae and thirty seven to Synurales. Spiniferomonas cornuta, Paraphysomonas sp., Mallomonas hexareticulata, M. ceylanica, M. plumosa, M. ocellata, M. elevata and M. spinosa are reported for the first time from China. A new species, Paraphysomonas hainanensis Wei &amp; Kristiansen, was found in a pond in Haikou City, Hainan Province. An amended description of Mallomonas spinosa Gusev is proposed. The sampled localities are situated in the tropical and subtropical region of southern China. There is great similarity in the geographical distribution of the observed silica‐scaled chrysophytes with that of east Asia, southeast Asia and south Asia, and their ecological characteristics are discussed.

Partitioning evapotranspiration in an intact forested watershed in southern China

AbstractAccurate estimates and partition of evapotranspiration (ET) are difficult but critical for understanding terrestrial ecosystem processes and primary productivity. A multi‐year, multi‐technique study including the catchment water balance (12 years), a semi‐empirical ET model (9 years), eddy covariance (9 years), canopy water balance (1 year) and sap flow (1 year) techniques was conducted to measure ET and its components within an intact forested watershed in the subtropical region of southern China. Over a 9‐year period, the estimates of annual ET using the catchment water balance, eddy covariance and semi‐empirical ET model techniques were in close agreement, averaging 809.9 ± 62.8, 803.8 ± 36.6 and 801.6 ± 46.9 mm per year, respectively, amounting to an average of approximately 50.2% of the mean annual precipitation. Qualitative similarities in seasonal and diurnal variation were observed between the sap flow and eddy covariance estimates of water flux. Sap flow estimates of transpiration were approximately 60.2% of the annual ET estimated with the eddy covariance technique. Interception evaporation was 31.7% of the total ET and was demonstrated to be a main contributor of total evaporation. Soil evaporation was calculated to be approximately 8.1% of the total annual ET and 4.7% of the annual precipitation. Furthermore, daytime transpiration exhibited a logarithmic increase with increases in the vapour pressure deficit (VPD) on daily scale in both the wet and dry seasons, and tended to level off when the VPD was &gt;1 kPa because of the stomatal regulation of transpiration. Correspondingly, nighttime sap flow amounted to an average of approximately 6% of the total daily sap flow, and no significant correlations with the VPD, air temperature or relative humidity were found. Essential differences among the mechanisms of evaporation and transpiration were demonstrated, suggesting that soil evaporation is primarily a climate‐driven process, with air temperature and wind speed as the predominant driving forces. Copyright © 2014 John Wiley &amp; Sons, Ltd.