Subsurface Flow Constructed Wetlands Research Articles

Understanding the interaction between soil ORP and enzyme activity: How does wetland type affect constructed wetland performance for the advanced treatment of swine wastewater?

Constructed wetlands (CWs) are widely used for the advanced treatment of swine wastewater. Wetland type is the most critical parameter for engineering applications; therefore, the performance of horizontal/vertical subsurface flow CWs (HSFCWs and VSFCWs) was explored over one year of operation. The effects of five antibiotics (40 μg/L each), copper (Cu, 1.0 mg/L), and their combination on CW performance and soil oxidation–reduction potential (ORP) and enzyme activity were investigated. The annual removal efficiencies of HSFCWs and VSFCWs for total organic carbon (TOC), nitrogen, and phosphorus were 72.03 %–84.49 % and 87.15 %–90.72 %, for antibiotics were 91.35 %–99.52 % and 87.33 %–99.57 %, and for Cu were 97.37 %–97.44 % and 95.52 %–96.85 %, respectively. The CWs removed antibiotics through substrate adsorption, plant absorption, and biodegradation. Total antibiotic residues in HSFCW and VSFCW soils ranged from 6.29 to 17.31 and 24.57 to 589.07 μg/kg, respectively, and enrofloxacin and roxithromycin posed medium and high risks in VSFCW non-rhizosphere soil. The amount of residual antibiotics in Phragmites australis parts was in the order of fibrous roots > rhizomes > aboveground. ORP and enzyme activities were greater in rhizosphere soil than in non-rhizosphere soil and greater in the VSFCWs than in the HSFCWs. CW performance and soil enzyme activities decreased under the stress of antibiotics and coexistence of antibiotics and Cu. These findings advance our knowledge of CW design, elucidate the unique advantages of CWs, and offer efficient operation strategies for swine wastewater advanced treatment, helping researchers recognize the adverse effects of antibiotics on CWs.

Enhancing Greywater Treatment: High-Efficiency Constructed Wetlands with Seashell and Ceramic Brick Substrates

Constructed wetland (CW) systems have been recognized as a sustainable technology for wastewater treatment that can be easily integrated into the local natural environment, offering both low cost and high efficiency. In this study, synthetic greywater was treated using a vertical subsurface flow CW operated in batch mode with 7-day cycles across two phases, operated in parallel: I, non-vegetated, and II, vegetated, with Echinodorus subalatus. The mixed filter bed was composed of seashells, ceramic brick fragments, and sand. No statistically significant differences (p > 0.05) were observed between the non-vegetated and vegetated phases for most parameters. The removal efficiencies of organic matter, anionic surfactants, and total phosphorus in the non-vegetated versus vegetated phases were (91.0 ± 3.8)% versus (94.0 ± 1.1)%; (71.9 ± 14.1)% versus (60.0 ± 9.5)%; and (35.2 ± 4.6)% versus (40.2 ± 15.5)%, respectively. Phosphorus removal exceeded values reported in the literature for both phases, primarily due to the calcium present in the seashells, which increased the electrical conductivity and hardness of the effluent compared to the influent. The macrophyte exhibited leaf desiccation, possibly due to contact with greywater and its young age (30 days), which may have negatively impacted the system’s performance during the vegetated phase.

Treatment of Domestic Wastewater in Colombia Using Constructed Wetlands with Canna Hybrids and Oil Palm Fruit Endocarp

Untreated domestic wastewater from rural areas poses significant risks to ecosystems and human health. Constructed wetlands (CWs) are a viable alternative for this wastewater treatment, enhancing nitrogen removal using substrates as carbon sources. This process is particularly beneficial for wastewater with low carbon-to-nitrogen (C/N) ratios, making the treated water suitable for agricultural irrigation. In this study, a Horizontal Subsurface Flow CW (HSF-CW) was evaluated using Canna hybrids and a mixed substrate of gravel and endocarp from oil palm fruit (EOP) as a carbon source to leverage its abundance in the region. It was also determined that the effluent complies with the permissible limits set by Resolution 1207 of 2014 from the Colombian Ministry of Environment and Sustainable Development, which establishes environmental standards for wastewater treatment to ensure environmental protection and enable safe reuse in agricultural irrigation. The key parameters analyzed included organic contaminants, heavy metals, nutrients, and microbiological indicators. Removal efficiencies of up to 91%, 94%, 98%, 52%, 73%, 78%, and 75% were achieved for BOD, TSS, total phosphorus, nitrates, nitrites, ammonium, and total nitrogen, respectively, demonstrating the CW’s strong performance in contaminant removal and meeting most standards for agricultural irrigation. Although the carbon source was not highly efficient, the overall system performance supports its viability for improving water quality and promoting sustainable agricultural practices in rural areas.

Exploring the role of phosphorus in the clogging formation of subsurface flow constructed wetland by two-dimensional visualization technique

Phosphorus (P) is associated with microbial activity and can be retained in constructed wetland (CW) through adsorption and chemical precipitation. However, its potential impact on clogging remains poorly studied. In this study, a two-dimensional (2D) visualization technique was utilized to investigate the effect of P on clogging process inside subsurface flow constructed wetland (SSF-CW) during the operation. Tracer images analysis showed that the preferential flow pathway proportions of P-containing group (P-CW) and P-free group (C-CW) were 30.38 ± 1.07 % and 9.72 ± 0.47 %, respectively. The fractal dimension of preferential flow pathways in C-CW (1.57 ± 0.02) was lower than in P-CW (1.70 ± 0.03), indicating that the preferential flow pathways in P-CW exhibited more diversion and tortuosity. The removal efficiencies of both CWs exhibited an initial increase followed by a decrease, with P-CW generally demonstrating higher pollutant removal efficiencies. In P-CW, the primary pathway for P removal was chemical precipitation dominated by apatite P (CaP). Furthermore, the addition of P altered the composition of extracellular polymeric substances (EPS) and reduced the stability of microbial aggregates, resulting in higher effective porosity. This study provides new insights into the role of P in the clogging of SSF-CW and would contribute to optimizing CW clogging management.

Novel overlapping constructed wetlands with water drops reoxygenation and lightweight fillers for decentralized wastewater treatment

Constructed wetlands (CWs), crucial for the rural decentralized wastewater treatment, have encountered limitations in nutrient removal efficiency and require extensive land area. This study has constructed a novel overlapping horizontal subsurface flow CWs (OLCWs). Remarkably, OLCWs with mixed lightweight fillers (M-OLCWs) exhibited a significant enhancement in total nitrogen (TN) removal efficiency (88–91 %) in different hydraulic loading rates compared to single filler OLCWs (48–62 %). This significant enhancement can be attributed to the lightweight fillers, which have higher abundances and diversity of nitrogen related microorganisms. The treatment dynamics revealed that the second stage exhibited an excellent TN removal efficiency (73–75 %) attributed to sufficient dissolved oxygen concentration by water drops reoxygenation. The research reveals that M-OLCWs, by utilizing water drops reoxygenation and lightweight fillers, not only enhance pollutant treatment efficiency but also reduce required land area, thereby offering a sustainable solution for rural decentralized wastewater treatment.

Novel lanthanum and waste lye modified fly ash zeolite for nitrogen and phosphorus removal: mechanisms and application in the constructed wetland

Excess inputs of nitrogen (N) and phosphorus (P) can lead to imbalance in water ecosystems and thus trigger eutrophication. In this study, a novel Lanthanum and waste lye modified zeolite synthesized from fly ash (LZFA) was prepared and used as a modified substrate for constructed wetland (CW) to enhance N and P removal. Single-factor and response surface methodology (RSM) were used to optimize the preparation process. The results showed that the maximum adsorption capacities of N and P were 17.26 mg/g and 21.48 mg/g, respectively. Compared to solely zeolite synthesized from fly ash (ZFA), the decreased N sorption capacity in LZFA was attributed to a reduction in the surface negative charge and cation exchange capacity. The mechanism of P adsorption was attributed to the formation of La-O-P monodentate, bidentate mononuclear or bidentate binuclear inner-sphere complexation. Meanwhile, the introduction of Ca from waste lye was also involved in the P reaction. The N and P removal rates of LZFA modified subsurface flow constructed wetland (SFCW) were 2.67 % and 7.33 % higher than SFCW modified with gravel. In practical production, if a circular chain from coal ash production to use for green plant fertilizer can be established, the cost of treating P can be significantly reduced.

Study Filter Materials for Vertical Subsurface Flow Constructed Wetland to Treat Wastewater from the Da Mai Noodle Handicraft Village in Bac Giang Province

Vertical subsurface flow-constructed wetlands (VSF CW) are evaluated in this research as a potentially effective and cost-efficient solution for treating wastewater from Da Mai noodle handicraft village in Bac Giang province. Due to the high pollution load in this type of wastewater, there are concerns about clogging and the stability of the technology. In this study, we explored different sizes of limestone and gravel for the five medium composition formulas in the VSF CW system. The experiments were conducted over a period of three months. We used ANOVA, one-sample T-test, and Pearson correlation analysis methods to evaluate the experimental results, with statistical significance set at 0.05. The results showed a linear correlation between medium size, hydraulic conductivity, and treatment efficiency (p < 0.05). Smaller medium sizes had higher pollutant removal efficiency but lower porosity and hydraulic conductivity. For the same sizes, limestone demonstrated higher treatment efficiency compared to gravel (p < 0.05). The Cp5 formula (comprising sand, 1×2 cm limestone, and 3×5 cm gravel) was selected as the best filter medium for VSF CW, achieving COD, TSS, TN, and TP removal efficiencies of 86.02 ± 1.71%, 81.15 ± 2.24%, 64.46 ± 2.23%, and 69.76 ± 2.68%, respectively. After three months of operation, the treated wastewater consistently met QCVN 40:2011/BTNMT Column B standards, and there were no significant differences in hydraulic conductivity (p > 0.05), indicating stable operation of the VSF CW.

Quantification of hydraulic characteristics and validation of CFD simulation of subsurface flow constructed wetlands using tracer

The analysis of residence time distributions (RTDs) is of great significance in the hydraulic characterization of subsurface flow constructed wetlands (SSF-CWs), and tracer experiments and simulations are generally used methods to obtain RTDs. This study developed a computational fluid dynamics (CFD) model involving two tracer tracking approaches in order to understand and quantify the flow behavior in SSF-CW. To validate the CFD model, RTDs were obtained from bench-scale impulse response tests with finite-constant injection of tracer, and the hydraulic indexes were corrected by introducing tracer injection time and nominal residence time in the formulae. The shape similarities of RTDs between the CFD model, the gamma model and observation were evaluated by a dynamic time warping algorithm, where the CFD model incorporating advection, diffusion and dispersion processes had the highest shape similarity (0.94–0.98). The proposed CFD model gave the tracer the same velocity field as the fluid, characterizing more accurate hydraulic indexes compared to the gamma model. The CFD model is appropriate for design optimization prior to full-scale construction since it saves time and is environmentally benign. It also gives visualization of the tracer trajectory, which aids in understanding contaminant fate and transport within the SSF-CW.

Response characteristics of plants and pollutant removal in subsurface flow constructed wetlands under resting operation

The stable operation of subsurface flow (SSF) constructed wetlands (CWs) depends on the adopted maintenance and management strategies. To determine a suitable resting period for SSF CWs, this study investigated and compared the impacts of three resting periods on plant physiological metabolism, wetland purification effectiveness, and microbial responses. The results revealed that resting operations induced morphological adaptations in wetland plants, significantly enhanced the root-shoot ratio and specific root length. A 2-d resting period enhanced oxygen secretion from plant roots, which was beneficial for SSF CW reoxygenation, leading to significant increases in the average removal rates of chemical oxygen demand and total phosphorus. After the resting operation, the inorganic phosphorus solubilization and transporters function of rhizosphere microorganisms were enhanced, resulting in efficient phosphorus removal. The functional genes related to nitrification, nitrogen fixation, and assimilation were increased, and a 2–5 d resting period facilitated the aerobic nitrification in SSF CWs. Inversely, an excessively long resting (>5 d) significantly inhibits plant and microbial activity, resulting in a deteriorated pollutant removal performance. Plants regulate rhizosphere microorganisms by adjusting root traits, which are the key factors and important reference indicators guiding the formulation of the operation and maintenance strategies for SSF CWs during resting periods.

Treatment of pharmaceutical industry wastewater for water reuse in Jordan using hybrid constructed wetlands

Developing cost-efficient wastewater treatment technologies for safe reuse is essential, especially in developing countries simultaneously facing water scarcity. This study developed and evaluated a hybrid constructed wetlands (CWs) approach, incorporating tidal flow (TF) operation and utilising local Jordanian zeolite as a wetland substrate for real pharmaceutical industry wastewater treatment. Over 273 days of continuous monitoring, the results revealed that the first-stage TFCWs filled with either raw or modified zeolite performed significantly higher reductions in Chemical Oxygen Demand (COD, 58 %–60 %), Total Nitrogen (TN, 32 %–37 %), and Phosphate (PO4, 46 %–64 %) compared to TFCWs filled with normal sand. Water quality further improved after the second stage of horizontal subsurface flow CWs treatment, achieving log removals of 1.09–2.47 for total coliform and 1.89–2.09 for E. coli. With influent pharmaceutical concentrations ranging from 275 to 2000 μg/L, the zeolite-filled hybrid CWs achieved complete removal (>98 %) for ciprofloxacin, ofloxacin, erythromycin, and enrofloxacin, moderate removal (43 %–81 %) for flumequine and lincomycin, and limited removal (<8 %) for carbamazepine and diclofenac. The overall accumulation of pharmaceuticals in plant tissue and substrate adsorption accounted for only 2.3 % and 4.3 %, respectively, of the total mass removal. Biodegradation of these pharmaceuticals (up to 61 %) through microbial-mediated processes or within plant tissues was identified as the key removal pathway. For both conventional pollutants and pharmaceuticals, modified zeolite wetland media could only slightly enhance treatment without a significant difference between the two treatment groups. The final effluent from all hybrid CWs complied with Jordanian treated industry wastewater reuse standards (category III), and systems filled with raw or modified zeolite achieved over 95 % of samples meeting the highest water reuse category I. This study provides evidence of using hybrid CWs technology as a nature-based solution to address water safety and scarcity challenges.

Influence of vegetation and substrate type on removal of emerging organic contaminants and microbial dynamics in horizontal subsurface constructed wetlands

Constructed wetlands (CWs) offer an efficient alternative technology for removing emerging organic contaminants (EOCs) from wastewater. Optimizing CW performance requires understanding the impact of CW configuration on EOC removal and microbial community dynamics. This study investigated EOC removal and microbial communities in horizontal subsurface flow (HSSF) CWs over a 26-month operational period. Comparison between tuff-filled and gravel-filled CWs highlighted the superior EOC removal in tuff-filled CWs during extended operation, likely caused by the larger surface area of the tuff substrate fostering microbial growth, sorption, and biodegradation. Removal of partially positively charged EOCs, like atenolol (29–98 %) and fexofenadine (21–87 %), remained constant in the different CWs, and was mainly attributed to sorption. In contrast, removal rates for polar non-sorbing compounds, including diclofenac (3–64 %), acyclovir (9–85 %), and artificial sweeteners acesulfame (5–60 %) and saccharin (1–48 %), seemed to increase over time due to enhanced biodegradation. The presence of vegetation and different planting methods (single vs. mixed plantation) had a limited impact, underscoring the dominance of substrate type in the CW performance. Microbial community analysis identified two stages: a startup phase (1–7 months) and a maturation phase (19–26 months). During this transition, highly diverse communities dominated by specific species in the early stages gave way to more evenly distributed and relatively stable communities. Proteobacteria and Bacteroidetes remained dominant throughout. Alphaproteobacteria, Acidobacteria, Planctomycetes, Salinimicrobium, and Sphingomonas were enriched during the maturation phase, potentially serving as bioindicators for EOC removal. In conclusion, this study emphasizes the pivotal role of substrate type and maturation in the removal of EOCs in HSSF CW, considering the complex interplay with EOC physicochemical properties. Insights into microbial community dynamics underscore the importance of taxonomic and functional diversity in assessing CW effectiveness. This knowledge aids in optimizing HSSF CWs for sustainable wastewater treatment, EOC removal, and ecological risk assessment, ultimately contributing to environmental protection.

Greenhouse gases emissions and carbon budget estimation in horizontal subsurface flow constructed wetlands with different plant species

Constructed wetlands (CWs) are pivotal for wastewater treatment due to their high efficiency and numerous advantages. The impact of plant species and diversity on greenhouse gas (GHG) emissions from CWs requires a more comprehensive evaluation. Moreover, controversial perspectives persist about whether CWs function as carbon sinks or sources. In this study, horizontal subsurface flow (HSSF) CWs vegetated with Cyperus alternifolius, Typhae latifolia, Acorus calamus, and the mixture of these three species were constructed to evaluate pollutant removal efficiencies and GHG emissions, and estimate carbon budgets. Polyculture CWs can stably remove COD (86.79 %), NH4+-N (97.41 %), NO3−-N (98.55 %), and TP (98.48 %). They also mitigated global warming potential (GWP) by suppressing N2O emissions compared with monoculture CWs. The highest abundance of the Pseudogulbenkiania genus, crucial for denitrification, was observed in polyculture CWs, indicating that denitrification dominated in nitrogen removal. While the highest nosZ copy numbers were observed in CWs vegetated with Cyperus alternifolius, suggesting its facilitation of denitrification-related microbes. Selecting Cyperus alternifolius to increase species diversity is proposed for simultaneously maintaining the water purification capacity and reducing GHG emissions. Carbon budget estimations revealed that all four types of HSSF CWs were carbon sinks after six months of operation, with carbon accumulation capacity of 4.90 ± 1.50 (Cyperus alternifolius), 3.31 ± 2.01 (Typhae latifola), 1.78 ± 1.30 (Acorus calamus), and 2.12 ± 0.88 (polyculture) kg C/m2/yr. This study implies that under these operation conditions, CWs function as carbon sinks rather than sources, aligning with carbon peak and neutrality objectives and presenting significant potential for carbon reduction efforts.

Enhancement of nitrates removal in pilot-scale treatment wetland systems

ABSTRACT The primary objective of the present study is to enhance the denitrification process in constructed wetlands (CWs), employed as an ecological and natural technology for wastewater treatment. Eco-friendly approaches have been tested to improve the nitrate removal efficiency in pilot-scale subsurface flow CWs. Initially, the system’s performance was enhanced by using a natural substrate, namely cork, as a filter medium. Another approach is bio-inoculation into the rhizosphere of a bacterial strain with the potential capacity for nitrate removal, thereby reinforcing the synergy between the plant and microorganisms. Finally, the approaches were combined, and nitrate removal was assessed. The main results showed that the most effective nitrate removal from wastewater was achieved with cork (80%) compared to gravel (60%). Bio-inoculation into the rhizosphere of denitrifying bacteria significantly improved nitrate removal. Indeed, the nitrate removal rate by the inoculated CW filled with gravel, was higher than the non-inoculated system, with rates of 78% and 60%, respectively. Moreover, the highest removal rate of 99.77% was obtained with the inoculated system filled with cork.

Application of New Filling Material Based on Combined Heat and Power Waste for Sewage Treatment in Constructed Wetlands.

The filling of constructed wetlands (CWs) affects the efficiency of sewage treatment and proper operation. Mineral aggregates are most often used as filling materials. Significant environmental burdens from mineral mining operations justify the search for waste fill. This study aimed to determine the possibility of increasing the efficiency of CW by using a Certyd aggregate as a new filling. Certyd is produced in the sintering process of coal ash, a waste from combined heat and power (CHP) plant operation. Comprehensive two-year studies were conducted using two real-scale subsurface vertical flow (SS VF) CWs supplied with domestic sewage. One bed was filled with a Certyd and the other was filled with appropriate fractions of a mineral aggregate. Both beds worked in parallel, and to compare their effectiveness, account seasonality was taken into account. The SS-VF Certyd-filled bed achieved an average efficiency of 88.0% for biological oxygen demand (BOD5), 80.2% for chemical oxygen demand (COD), 80.4% for suspended solids (SSs), 80.2 for ammonia nitrogen (N-NH4), 72.2% for total nitrogen (TN), and 55.3% for total phosphorus (TP), while the gravel-filled bed achieved 84.5%, 77.0%, 86.9%, 74.2%, 69.4%, and 57.8% for the whole research period, respectively. A higher effect of the removed unit load was achieved in the bed filled with Certyd (36.2 g BOD5 m-2 d-1, 50.0 g COD m-2 d-1, 5.88 g SS m-2 d-1, 7.1 g TN m-2 d-1, 7.9 g N-NH4 m-2 d-1, 0.79 g TP m-2 d-1) compared to the gravel-filled bed (34.7 g BOD5 m-2 d-1, 47.0 g COD, 6.35 g SS m-2 d-1, 6.9 g TN m-2 d-1, 7.3 g m-2 d-1 N-NH4, 0.83 g TP m-2 d-1).

The comparison between different types of constructed wetlands for biochemical oxygen demand removal efficiency

This research shows efficiency of constructed wetlands (CWs) to purify waste water in the case of Biochemical Oxygen Demand (BOD). CWs such as surface flow (SF), subsurface flow (SSF), and hybrid (HYB) systems have been compared to provide an analysis about which system has better performance for BOD removal efficiency. Data were collected from different scientific articles and from all over the world. Meta-analysis technique was employed to aggregate data from scientific sources, facilitating hypothesis testing, and comparisons between different types of CWs. All the systems of CWs show satisfactory removal efficiency. HYB systems are shown to be the most efficient. One-way analysis of variance (ANOVA) has been applied to analyze differences between respective CWs using R software. It shows that there is a statistically significant difference between different types of CWs. Post-hoc Tukey?s Honestly Significant Different (HSD) analysis demonstrates a statistically significant difference between SF and HYB systems in the case of BOD removal efficiency. Also, Post-hoc Tukey?s HSD shows statistically significant difference between SSF and SF CWs. On the other hand, Post-hoc Tukey?s HSD does not show statistically significant difference between HYB and SSF CWs. The significant reduction rates for BOD removal efficiency, demonstrates that CWs can be used to diminish this kind of pollution.

SIGNIFICANCE OF CONSTRUCTED WETLANDS IN COMBATING POLLUTANTS FROM WASTEWATER: A SUSTAINABLE DEVELOPMENT PERSPECTIVE

Water contamination is the greatest hazard to public health. Addressing water scarcity and protecting accessible water sources necessitates the effective treatment of wastewater. This makes the use of sustainable solutions such as constructed wetlands (CWs) essential. CWs leverage natural processes involving wetland vegetation, soils, and microbial communities. This study evaluates the efficiency of a horizontal sub-surface flow CW, established with local plants at Hudiara drain, in removing pollutants such as Biochemical Oxygen Demand (BOD), Turbidity, Nitrates, Phosphates, and pH, across different months. The study reveals that while temperature and precipitation rates influence the CW's efficacy, the linear regression model indicates a strong correlation between phosphorus and BOD levels with precipitation. However, nitrates are sensitive to temperature, and turbidity is influenced by both temperature and precipitation within certain limits. Additional factors impacting CW performance include wastewater characteristics, design flow, and wetland location. When compared with Pakistan Environmental Quality Standards (PEQS), it is concluded that CWs are effective in wastewater treatment. By constructing CWs along the banks of wastewater drains, treated water from the outlet chamber can be collected and redirected, offering a viable solution to water scarcity challenges.

The critical role of microplastics in the fate and transformation of sulfamethoxazole and antibiotic resistance genes within vertical subsurface-flow constructed wetlands

Constructed wetlands (CWs) are reservoirs of microplastics (MPs) in the environment. However, knowledge about the impact of MPs on antibiotic removal and the fate of antibiotic resistance genes (ARGs) is limited. We focused on sulfamethoxazole (SMX) as a representative compound to examine the effects of MPs on SMX removal and the proliferation and dissemination of two SMX-related ARGs (sul1 and sul2) in vertical subsurface-flow CW (VFCW) microcosm. The presence of MPs in the substrate was found to enhance the proliferation of microorganisms owing to the large specific surface area of the MPs and the release of dissolved organic carbon (DOC) on MP surfaces, which resulted in a high SMX removal ranging from 97.80 % to 99.80 %. However, the presence of MPs promoted microbial interactions and the horizontal gene transfer (HGT) of ARGs, which led to a significant increase in the abundances of sul1 and sul2 of 68.47 % and 17.20 %, respectively. It is thus imperative to implement rigorous monitoring strategies for MPs to mitigate their potential ecological hazards.

Advantages of using a carbon-rich substrate in a constructed wetland for agricultural water treatment: Carbon availability and biota development

Proper bed substrate selection is essential for the growth of microorganisms and plants, which are key elements in constructed wetlands (CWs) performance. This is even more important in CWs that treat irrigated agricultural drainage water characterised by a high nitrogen (N), but low carbon (C) and phosphorus (P) concentration. This nutrient imbalance compromises biota activity and limits water treatment processes. Three substrates were tested in a CW field-scale pilot plant for 2 years: gravel (100%) and two mixed substrates, gravel + 30% natural wetland soil and gravel + 10% biochar. While gravel is one of the most commonly used substrates in subsurface flow CWs, soil has hardly been employed, and field-scale biochar application studies are scarce. We analysed the effect of adding a C-rich substrate to gravel on both nutrient imbalance correction and the biotic performance of CWs. Adding natural soil or biochar increased P availability in beds. However, the dissolved organic C concentration in interstitial water was only enhanced by soil addition. Unlike gravel, microbial density and activity, and Phragmites australis plant growth, were higher in beds with soil, followed by those with biochar. A similar pattern was observed for plant tissue quality. Although biochar proved positive for biota, it had a short-term effect as a source of C and P. Therefore, employing a suitable gravel-soil mixture provides notable advantages for the irrigated agricultural water treatment challenge. Even after considering some limitations, our results represent an important step to design CWs that treat this wastewater type and for eutrophication control in sustainable agroecosystems.

Plant development alters the nitrogen cycle in subsurface flow constructed wetlands: Implications to the strategies for intensified treatment performance

Plant development greatly influences the composition structure and functions of microbial community in constructed wetlands (CWs) via plant root activities. However, our knowledge of the effect of plant development on microbial nitrogen (N) cycle is poorly understood. Here, we investigated the N removal performance and microbial structure in subsurface flow CWs at three time points corresponding to distinct stages of plant development: seedling, mature and wilting. Overall, the water parameters were profoundly affected by plant development with the increased root activities including radial oxygen loss (ROL) and root exudates (REs). The removal efficiency of NH4+-N was significantly highest at the mature stage (p < 0.01), while the removal performance of NO3−-N at the seedling stage. The highest relative abundances of nitrification- and anammox-related microbes (Nitrospira, Nitrosomonas, and Candidatus Brocadia, etc.) and functional genes (Amo, Hdh, and Hzs) were observed in CWs at the mature stage, which can be attributed to the enhanced intensity of ROL, creating micro-habitat with high DO concentration. On the other hand, the highest relative abundances of denitrification- and DNRA-related microbes (Petrimonas, Geobacter, and Pseudomonas, etc.) and functional genes (Nxr, Nir, and Nar, etc.) were observed in CWs at the seedling and wilting stages, which can be explained by the absence of ROL and biological denitrification inhibitor derived from REs. Results give insights into microbial N cycle in CWs with different stages of plant development. More importantly, a potential solution for intensified N removal via the combination of practical operation and natural regulation is proposed.

Inducing root redundant development to release oxygen: An efficient natural oxygenation approach for subsurface flow constructed wetland

Dissolved oxygen (DO) is a limiting factor affecting the purification efficiency of subsurface flow (SSF) constructed wetlands (CWs). To clarify the causes of oxygen environments and the response characteristics of plant oxygen release (POR) in SSF CWs, this study set three oxygen source treatments by limiting atmospheric reaeration (AR) and influent oxygen (IO) and compared the differences in plant physiological metabolism, DO distribution characteristics, and the purification effect of the SSF CWs at different depths. The results showed that limiting exogenous oxygen stimulated root redundancy of the wetland plants. The root volume and proportion of fibrous roots of the wetland plants increased significantly (p < 0.05). When only the POR existed, the root zone DO increased significantly to 2.05–4.37 mg/L (p < 0.05), and was positively correlated with the TN and TP removal rates (p < 0.05). Additionally, in the presence of POR only, the average removal rates of TN and TP in the top layer were 86.5% and 76.9%, respectively. The proportion of fibrous roots, root zone DO, and root-shoot ratio were key factors promoting the purification effect of the SSF CWs under limited exogenous oxygen sources. Enhancing POR by inducing root redundancy enhanced nitrification (hao, pmoABC-amoABC), plant absorption, and assimilation-related functional genes (nrtABC, nifKDH), and enriched nitrogen and phosphorus removal bacteria, such as Flavobacterium and Zoogloea. This consequently improved pollutant removal efficiency. Inducing root redundancy to strengthen POR produced an aerobic environment in the SSF CWs. This ensures the efficient and stable operation of the SSF CW and is an effective approach for natural oxygenation.