Subsequent Developmental Stages Research Articles

Chinese Consumers' Satisfaction with Online Shopping Platforms

With the rapid development of Internet technology, China's online shopping industry is also expanding rapidly, becoming an important part of global e-commerce. This article investigates Chinese consumers' satisfaction levels with Taobao, Jingdong (JD.com), and Pinduoduo, three of the country's most popular e-commerce sites. The article reviews the historical development of these platforms, their market positioning, and some of the unique spending habits of Chinese consumers. This paper analyzes several key factors that affect consumer satisfaction, including delivery accuracy, price, information quality, payment convenience, and security. The way that these aspects are handled by each platform greatly affects the buying experience of users. Technological innovations on platforms such as Taobao, such as AI-driven recommendations, have dramatically improved user experience and satisfaction. Jingdong has improved its delivery efficiency and customer satisfaction by building its own logistics system, and Pinduoduo's logistics are both slightly inadequate in comparison. However, Jingdong's logistics during peak hours still needs to be improved. The paper also points out that while price is a very important factor for consumers when choosing a platform, Pinduoduo still needs to find a balance between low price and service quality. Lastly, future studies can explore how cross-border e-commerce and the behavioral inclinations of various user groups could impact the platform's subsequent development stage. These findings suggest that continued attention to these factors can provide online shopping platforms with strategies to increase consumer satisfaction and improve service quality, as well as provide new ideas for academic research.

Systemic deficits in lipid homeostasis promote aging-associated impairments in B cell progenitor development.

Organismal aging has been associated with diverse metabolic and functional changes across tissues. Within the immune system, key features of physiological hematopoietic cell aging include increased fat deposition in the bone marrow, impaired hematopoietic stem and progenitor cell (HSPC) function, and a propensity towards myeloid differentiation. This shift in lineage bias can lead to pre-malignant bone marrow conditions such as clonal hematopoiesis of indeterminate potential (CHIP) or clonal cytopenias of undetermined significance (CCUS), frequently setting the stage for subsequent development of age-related cancers in myeloid or lymphoid lineages. At the systemic as well as sub-cellular level, human aging has also been associated with diverse lipid alterations, such as decreased phospholipid membrane fluidity that arises as a result of increased saturated fatty acid (FA) accumulation and a decay in n-3 polyunsaturated fatty acid (PUFA) species by the age of 80 years, however the extent to which impaired FA metabolism contributes to hematopoietic aging is less clear. Here, we performed comprehensive multi-omics analyses and uncovered a role for a key PUFA biosynthesis gene, ELOVL2 , in mouse and human immune cell aging. Whole transcriptome RNA-sequencing studies of bone marrow from aged Elovl2 mutant (enzyme-deficient) mice compared with age-matched controls revealed global down-regulation in lymphoid cell markers and expression of genes involved specifically in B cell development. Flow cytometric analyses of immune cell markers confirmed an aging-associated loss of B cell markers that was exacerbated in the bone marrow of Elovl2 mutant mice and unveiled CD79B, a vital molecular regulator of lymphoid progenitor development from the pro-B to pre-B cell stage, as a putative surface biomarker of accelerated immune aging. Complementary lipidomic studies extended these findings to reveal select alterations in lipid species in aged and Elovl2 mutant mouse bone marrow samples, suggesting significant changes in the biophysical properties of cellular membranes. Furthermore, single cell RNA-seq analysis of human HSPCs across the spectrum of human development and aging uncovered a rare subpopulation (<7%) of CD34 + HSPCs that expresses ELOVL2 in healthy adult bone marrow. This HSPC subset, along with CD79B -expressing lymphoid-committed cells, were almost completely absent in CD34 + cells isolated from elderly (>60 years old) bone marrow samples. Together, these findings uncover new roles for lipid metabolism enzymes in the molecular regulation of cellular aging and immune cell function in mouse and human hematopoiesis. In addition, because systemic loss of ELOVL2 enzymatic activity resulted in down-regulation of B cell genes that are also associated with lymphoproliferative neoplasms, this study sheds light on an intriguing metabolic pathway that could be leveraged in future studies as a novel therapeutic modality to target blood cancers or other age-related conditions involving the B cell lineage.

Unmasking Student Dynamics: the Impact of COVID-19 on Social-Emotional Development and Learning Engagement

This study aimed to examine the impact of COVID-19 on the pattern of socialemotional development and learning engagement of Korean students. It employs latent profile analysis to categorize middle and high school students based on their social-emotional development (action-orientation, optimism, perseverance, relationship with adults) and learning engagement (cooperative learning, and self-directed learning). The analysis, conducted during the fall semester of 2019 and the spring semester of 2020 amid COVID-19 school closures, explores nuanced patterns and effects. The findings reveal distinct student groups with discernible differences across all factors both before and during school closure. Notably, the research suggests that early social-emotional development may influence subsequent developmental stages and that a student’s social-emotional skills correlate with cooperative and self-directed learning. Furthermore, the study highlights the pandemic’s varied impact on student groups, indicating that those with advanced social-emotional competencies and established learning practices were resilient to school closures. In contrast, ’average’ students faced challenges in cooperative and active learning during lockdown. The study underscores the need for targeted educational measures, particularly for at-risk students, and suggests proactive preparation for future pandemics.

Regulation of trehalose metabolism mediated by validamycin on chitin synthesis in Spodoptera frugiperda

AbstractTrehalase (TRE) is a key enzyme for degrading trehalose, which plays a vital role in the growth and development of insects. Although validamycin, a compound belonging to a class of efficient antibiotics and fungicides, can control pests by suppressing TRE activities, it remains unknown whether it acts on both trehalose and chitin metabolism in Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae), a major pest of maize (Zea mays L., Poaceae). This study investigated the changes in trehalose metabolism after validamycin treatment in S. frugiperda and its effects on the downstream chitin synthesis pathway. Compared with the control, S. frugiperda exhibited varying degrees of mortality after treatment with four concentrations of validamycin, showing a dose‐dependent increase in mortality rate. The mortality rates 24 and 48 h after treatment with 0.07 mg μL−1 validamycin were 35.6% and 42.2%, respectively, indicating the effective lethal concentration. Treatment with 0.07 mg μL−1 validamycin led to developmental delay, abnormal molting, and death in S. frugiperda, but it exerted no lasting effects on the survival rate, pupal weight, and phenotype during its subsequent developmental stages. At 24 h after validamycin treatment, TRE1 and TRE2 activities and glucose content decreased significantly, whereas the trehalose content increased significantly. Treatment with validamycin significantly upregulated TRE1 and TRE2 expression after 24 and 48 h and downregulated the mRNA expression of chitin synthase A and B genes. However, after 72 h, chitin content was not significantly affected. Hence, validamycin can destroy the dynamic transformation balance of trehalose and glucose by inhibiting the activities of the two TREs, and further affect the expression of downstream chitin synthase genes. These findings provide a theoretical basis for using TRE inhibitors to control S. frugiperda.

Purification and characterization of α-amylase from Acanthoscelides obtectus (Say) (Coleoptera: Chrysomelidae)

Acanthoscelides obtectus is one of the most notorious pests of stored kidney beans (Phaseolus vulgaris) worldwide. Kidney beans are an important source of food for these insects. α-Amylase is the main carbohydrate-digesting enzyme in animals including insects. In the current study, the biochemical analysis revealed higher α-amylase activity (U/ml) in 3rd and 4th larval instars but decreased gradually in subsequent developmental stages. However, the specific activity (U/mg) interestingly was highest in 1st instar and decreased in further developmental stages. During qualitative analysis of α-amylase using starch-agar and native PAGE, the maximum zone of starch lysis and a prominent band on the gel was observed in 3rd and 4th larval stages. The molecular mass of the native enzyme was also estimated and found to be 30.34 kDa. The crude α-amylase was further purified by ammonium sulfate precipitation, gel filtration on a Sephadex G-75, and ion exchange chromatography on the DEAE cellulose column. The purified amylase was found to be a monomer with a molecular mass of 15 kDa. The specific activity of the purified enzyme increased from 1.74 U/mg in the crude sample to 166.35 U/mg in the final purification step resulting in 95-fold purification with a yield of 11.14%. Further characterization of purified α-amylase revealed a pH optimum of 7.0 and a temperature optimum of 35 °C. Lineweaver-Burk plot analysis revealed Km and Vmax to be 0.09% and 3.3 U/mL, respectively. Oxalic acid, tannic acid, and HgCl2 significantly inhibited the enzyme, while the Na+, Ca++, and Mg++ ions acted as activators. In conclusion, the study revealed, the highest α-amylase activity in 3rd and 4th larval stages of Acanthoscelides obtectus followed by native and SDS PAGE resulting in molecular mass of 30.34 and 15 kDa respectively.

River-sea migration patterns of juvenile Japanese seabass influence migrations in subsequent stages

The Japanese seabass Lateolabrax japonicus, an important commercial fishery species, exhibits partial migration, where some individuals migrate upstream into rivers, and others inhabit estuarine and coastal areas within the same population. Although this migratory behavior has been documented in juvenile seabass, the prevalence of river use and the ecological significance of partial migration in subsequent developmental stages remain unclear. To understand the river use of subadult and adult Japanese seabass, we estimated the river use history using otolith Sr/Ca ratios from individuals captured in both marine and riverine environments. Based on hierarchical clustering with Dynamic Time Warping, Sr/Ca ratios at age 0 were clustered into three habitat use patterns: coastal resident, estuarine resident, and river migrant. Groups that used coastal and riverine habitats at age 0 tended to use coastal and riverine habitats, respectively, from spring to fall of age 1 and above, with overlapping distributions during winter. Subadult and adult river use patterns were linked to juvenile river use, indicating a carryover effect. Additionally, seasonal migration between the sea and rivers became less active with age, and the high growth sustained by river use also declined with age, suggesting that the benefits of river use are less for older (subadult and adult) seabass. This study highlights that juvenile habitat use experience drives subadult and adult partial migration of Japanese seabass, with individuals seeking environments adapted from past experiences. Our findings indicate that the seabass population consists of individuals adapted to various environments, from the sea to the rivers, maximizing the use of biological productivity.

Imidazole-thiadiazole hybrids: A multitarget de novo drug design approach, in vitro evaluation, ADME/T, and in silico studies.

A library of imidazole-thiadiazole compounds (1-24) was synthesized to explore their therapeutic applications. The compounds were subjected to meticulous in vitro evaluation against α-glucosidase, α-amylase, acetylcholinesterase (AChE), and butylcholinesterase (BChE) enzymes. Compounds were also investigated for antioxidant activities using cupric reducing antioxidant capacity (CUPRAC), ferric reducing antioxidant power (FRAP), and 1,1-diphenyl-2-picrylhydrazyl (DPPH) assays. Derivatives 5-7, 9-11, 18, and 19 displayed potent inhibitory activities with IC50 values of 1.4 ± 0.01 to 13.6 ± 0.01 and 0.9 ± 0.01 to 12.8 ± 0.02 µM against α-glucosidase, and α-amylase enzymes, respectively, compared to the standard acarbose (IC50 = 14.8 ± 0.01 µM). Compounds 11-13, 16, 20, and 21 exhibited potent activity IC50 = 8.6 ± 0.02 to 34.7 ± 0.03 µM against AChE enzyme, compared to donepezil chloride (IC50 = 39.2 ± 0.05 µM). Compound 21 demonstrated comparable inhibition IC50 = 45.1 ± 0.09 µM against BChE, compared to donepezil chloride (IC50 = 44.2 ± 0.05 µM). All compounds also demonstrated excellent antioxidant activities via CUPRAC, FRAP, and DPPH methods. Complementing the experimental studies, extensive kinetics, ADME/T, and molecular docking analysis were also conducted to unravel the pharmacokinetics and safety profiles of the designed compounds. These studies supported the experimental findings and facilitated the prioritization of hit candidates for subsequent stages of drug development.

Modeling climate influences on male survival in the highly polygynous southern elephant seal (Mirounga leonina)

AbstractThe Southern Ocean, its ecosystem, and inhabitants are vulnerable to climate‐mediated shifts in habitat conditions. These changes can affect marine predators in the long term through changes in the distribution, density, and quality of prey. To understand how these changes affect vital rates requires longitudinal studies integrating demography and environmental states. We analyzed 22 years of capture–mark–recapture data to quantify the influence of broad‐scale climate on the survival of male southern elephant seals from Macquarie Island, Tasmania, Australia. Weaning mass was incorporated as a quadratic predictor of survival to account for the known influence of maternal investment. First‐year survival was greatest for weaners weighing 160 kg. Subsequent developmental stages were grouped into juvenile, subordinate, and breeding adult states to reflect the known life history of individuals. We found increased juvenile and subordinate survival with positive values of the previous years' Southern Annular Mode. This is likely due to the northward shift of westerly winds increasing productivity and prey availability in the Sub‐Antarctic region. In contrast to females of this population, the Southern Oscillation Index did not affect male survival. In this highly polygynous mating system, sex‐dependent differences in foraging behavior may be causing the small number of successful breeding adult males to be less susceptible to external influences after reaching the minimum age and body size required to be socially competitive for mating. These extremely large bulls will breed with multiple females and thus make a disproportionate contribution to the gene pool of the next generation. Considering the susceptibility of males to climate influences is therefore highly relevant to assessing the future adaptive capability of the species.

Integration of UML Class Diagrams Based on Semantics and Structure

As a high-level reuse, software design reuse has received more and more attention because of its important impact on the subsequent development stages. Usually, the design models are typically represented as some graphs or diagrams, in which Unified Modeling Language (UML) class diagram is so widely used in software design that it has become the de facto standard. There has been some research on the reuse of UML class diagrams so far, mainly focusing on matching and retrieval. However, it is worth noting that there are many similar class diagrams modeling the same object or some related class diagrams modeling different aspects of the same object in the reuse repository. As a matter of fact, the primary step to achieve a high-quality reuse is to have high-quality software artifacts, so the well-designed UML class diagrams become a necessary resource for software design reuse. Therefore, it is necessary to integrate these class diagrams so that they have stronger modeling ability, and eliminating redundancy is another benefit of the integration. Up to now, there has been little discussion about the integration of class diagrams, so we propose an integration approach based on semantics and structure in this paper. The equivalent elements that can identify the semantically merged parts are defined, and the possible conflict items are listed from both semantic and structural aspects. The integration procedure composed of three stages is proposed, in which an approach combining semantic common class diagrams (SCCDs) and structural common graph sequence (SCGS) is combined to determine the merged parts, the integration issue of heterogeneous class diagrams is considered from the proposed abstract models, and the conflict resolution for each conflict item is described by examples. The experimental results show the effectiveness of our proposed integration approach.

Study on Static Mechanical Properties and Numerical Simulation of Coral Aggregate Seawater Shotcrete with Reasonable Mix Proportion

This study aims to explore the static mechanical characteristics of coral aggregate seawater shotcrete (CASS) using an appropriate mix proportion. The orthogonal experiments consisting of four-factor and three-level were conducted to explore an optimal mix proportion of CASS. On a macro-scale, quasi-static compression and splitting tests of CASS with optimal mix proportion at various curing ages employed a combination of acoustic emission (AE) and digital image correlation (DIC) techniques were carried out using an electro-hydraulic servo-controlled test machine. A comparative analysis of static mechanical properties at different curing ages was conducted between the CASS and ordinary aggregate seawater shotcrete (OASS). On a micro-scale, the numerical specimens based on particle flow code (PFC) were subjected to multi-level microcracks division for quantitive analysis of the failure mechanism of specimens. The results show that the optimal mix proportion of CASS consists of 700 kg/m3 of cementitious materials content, a water–binder ratio of 0.45, a sand ratio of 60%, and a dosage of 8% for the accelerator amount. The tensile failure is the primary failure mechanism under uniaxial compression and Brazilian splitting, and the specimens will be closer to the brittle material with increased curing age. The Brazilian splitting failure caused by the arc-shaped main crack initiates from the loading points and propagates along the loading line to the center. Compared with OASS, the CASS has an approximately equal early and low later strength mainly because of the minerals’ filling or unfilling effect on coral pores. The rate of increase in CASS is swifter during the initial strength phase and decelerates during the subsequent stages of strength development. The failure in CASS is experienced primarily within the cement mortar and bonding surface between the cement mortar and aggregate.

Fruit Quality and Plant Productivity of A Cherry Tomato (Solanum lycopersicum var. cerasiforme) Grown under Different Irrigation Regimes during the Reproductive Phase

The impact of water deficit severity and the specific growth stages at which water stress is applied have been found to have significant implications for both tomato yield and fruit quality. Recent findings have highlighted the influence of water deficits during the reproductive phase on tomato fruit quality. This study aimed to assess the effects of deficit irrigation on tomato fruit quality and overall plant productivity. Cherry tomatoes (Solanum lycopersicum var. cerasiforme) were grown in a greenhouse from August to November 2022. Three distinct watering regimes were implemented: Daily watering (T1), watering every 3 days (T2) and watering every 7 days (T3), starting from the flowering stage and continuing through subsequent plant development stages. The mean soil metric potentials (SMP) were −4.9, −29.7 and −52.8 kPa for T1, T2 and T3, respectively. Various traits of fruit size, fruit biomass, total soluble solids (TSS), water content, glucose content and lycopene content were measured. Additionally, the overall plant biomass and root-to-shoot ratio were evaluated. The results revealed that traits of the fruit size, such as diameter, length, volume and fresh weight, were most favorable when plants were subjected to the T2 watering regime, while higher and lower watering frequencies led to smaller fruit sizes. Interestingly, the TSS concentration had the most pronounced response to drought stress, indicating increased fruit sweetness. However, the fruit water content, glucose content and lycopene content remained unaffected by the different watering regimes. Furthermore, plants subjected to the T3 with the minimum SMP at −196.4 kPa exhibited enhanced development of the root system, prioritizing resource acquisition such as water and mineral nutrients over shoot components. In conclusion, this study provided valuable insights for agricultural practitioners, offering a range of alternatives that can inform optimal irrigation strategies that effectively enhance the quality of cherry tomato yields. HIGHLIGHTS Water deficits during the reproductive phase have a notable impact on tomato fruit quality Three distinct watering regimes, including daily watering (T1), watering every 3 days (T2) and watering every 7 days (T3), were implemented, with mean soil metric potentials for T1, T2 and T3 recorded at −4.9, −29.7 and −52.8 kPa, respectively T2 watering regime resulted in the most favorable fruit size (diameter, length, volume and fresh weight), while higher and lower watering frequencies led to smaller fruit sizes Total soluble solid concentration showed a pronounced response to drought stress, indicating increased fruit sweetness, while glucose content and lycopene content were unaffected by different watering regimes The study provides valuable insights for agricultural practitioners, suggesting alternative irrigation strategies to enhance the quality of cherry tomato yields GRAPHICAL ABSTRACT

Time and mechanism of formation of mud volcanoes in the South Caspian basin according to seismic data

There are 277 mud volcanoes located in the South Caspian basin and the Apsheron-Gobustan periclinal trough and the Lower Kura depression that open to it. On land there are 177 mud volcanic structures, from which steam, water, mud, gas and oil are constantly erupting at approximately 500 points. Mud volcanoes located in the Caspian Sea (about 100) also play an important role in the degassing (mainly methane) of the sedimentary layer of the earth’s crust. Based on the results of geological and geophysical research, it has been established that almost all mud volcanoes discovered on the territory of Azerbaijan are associated with hydrocarbon deposits. In the Oligocene – Lower Miocene, mainly in the Maikop time, a thick clayey stratum (Maikop) - source rocks – accumulated in the South Caspian depression. At the end of the Miocene, there was a sharp drop in erosion levels in this basin. As a result of the activity of several river systems (Pleo-Volga, Paleo-Kura, Paleo-Pirsagat, Paleo-Amu Darya) over the Maikop clay deposits, a system of alternating layers of low and high density was formed with a total thickness of sediments of eight kilometers or more. The Maikop clay formations buried under this multilayer system, possessing the properties of a non-Newtonian fluid, created intrusions in the overlying environment, which at subsequent stages of development of the sedimentation basin became eruptive channels of mud volcanoes. The results of research using 3D seismic data made it possible to identify the mechanism of formation of mud volcanism, which, according to the authors, is associated with the migration of hydrocarbons from oil and gas source rocks to reservoir rocks, with the channels of mud volcanoes being considered as the main migration routes. Geological interpretation of seismic data shows that the formation of mud volcanoes in the South Caspian basin supposedly began from the beginning of the Miocene and continued in parallel during subsequent periods of development of the sedimentation basin. As the thickness of the sedimentary cover increased, mud volcanoes periodically became active.

Gene Cloning, Heterologous Expression, and In Silico Analysis of Chitinase B from Serratia marcescens for Biocontrol of Spodoptera frugiperda Larvae Infesting Maize Crops.

Spodoptera frugiperda, the fall armyworm (FAW), is a highly invasive polyphagous insect pest that is considered a source of severe economic losses to agricultural production. Currently, the majority of chemical insecticides pose tremendous threats to humans and animals besides insect resistance. Thus, there is an urgent need to develop new pest management strategies with more specificity, efficiency, and sustainability. Chitin-degrading enzymes, including chitinases, are promising agents which may contribute to FAW control. Chitinase-producing microorganisms are reported normally in bacteria and fungi. In the present study, Serratia marcescens was successfully isolated and identified from the larvae of Spodoptera frugiperda. The bacterial strain NRC408 displayed the highest chitinase enzyme activity of 250 units per milligram of protein. Subsequently, the chitinase gene was cloned and heterologously expressed in E. coli BL21 (DE3). Recombinant chitinase B was overproduced to 2.5-fold, driven by the T7 expression system. Recombinant chitinase B was evaluated for its efficacy as an insecticidal bioagent against S. frugiperda larvae, which induced significant alteration in subsequent developmental stages and conspicuous malformations. Additionally, our study highlights that in silico analyses of the anticipated protein encoded by the chitinase gene (ChiB) offered improved predictions for enzyme binding and catalytic activity. The effectiveness of (ChiB) against S. frugiperda was evaluated in laboratory and controlled field conditions. The results indicated significant mortality, disturbed development, different induced malformations, and a reduction in larval populations. Thus, the current study consequently recommends chitinase B for the first time to control FAW.

«Бьюти-пространство» Боттичелли, поп-арт и гностики (к вопросу о становлении классического искусства)

&lt;p&gt;The article continues the series of publications by the author on the art of the Renaissance and the work of Botticelli. The author's concept of “classical art” allows us to establish closer connections with subsequent stages of artistic development, as well as with the Early Renaissance. The multi-level structure of Botticelli’s art and the “collage” nature of his works are substantiated. The “universality” of the classics correlates with the gaming strategies of Neoplatonism and Gnosticism. The features that bring the intellectual culture of the Renaissance closer to modern scientific and artistic trends are revealed. New contexts and parallels (Michael Psellus, Honore de Balzac, Arthur Schopenhauer, Alois Riegl, David Hockney, Roy Lichtenstein) allow us to re-evaluate the transformation of the Neoplatonic tradition in European culture. The author rejects rigid interpretations of Gnosticism and Neoplatonism in connection with the work of Botticelli, preferring an “aesthetic” or “poetic” reading. Within the framework of this essentially psychological approach, the author draws attention to the problem of “presence” (“life-likeness”), characteristic of both medieval and modern art. Social issues of interpretation of classical art are touched upon in the article in connection with elements of elitism in the culture of the Medici circle. The concept of “poetry”, developed by Petrarch and Boccaccio and going back to ancient prototypes, is fully consistent with this type of “court” culture, which played a decisive role in the development of classical art. The "decadence" of the late Quattrocento and the work of Botticelli as its highest expression were thus the result of very complex intellectual strategies that were continued during the High Renaissance and Mannerism. Particular attention in the article is paid to the revision of the usual views on the historiography of the Renaissance, the actualization of classical art historical concepts. The article returns to the problem of the origins of the semiotic concept of art. Pragmatism and the “irrational” concept of art (appealing to the unconscious) turn out to be quite compatible, that has particular theoretical significance for the revaluation of conventional approaches to the classical heritage.&lt;/p&gt;

Features of calculating the strength of gantry and ship cranes gear units in the probabilistic aspect

The performed study is the result of collecting statistical data on failures of port transshipment and floating cranes. It is found that many accidents of components and parts of crane mechanisms can be associated with an erroneous determination of design loads. In order to increase the strength reliability, numerous field tests were carried out in river and sea ports under various modes of transshipment for different types of cargo. Tests have shown that operational loads are random in nature, and their laws of distribution over time of individual crane mechanisms can be approximated by truncated normal Gauss laws, accounting for which at the stage of new design developments, as shown by the practice of performing strength fatigue calculations, is not possible. In the article, in order to use the obtained laws at the design stage, it is proposed to replace the representation of operational loads with initial moments that are common to the entire crane mechanism, and transfer individual characteristics consideration such as stress concentration coefficients, fatigue curve parameters, operating time or test base, to subsequent stages of machine components development. For practical testing of the operational loads representation by the normal Gauss laws, a critical analysis of their initial moments, which makes it possible to identify inconsistencies in the representations of variable loads proposed for strength calculation, is given. For the corrected truncated normal load distribution law, the initial moments, the value of the order of which corresponds to the formulas structure used in calculating fatigue resistance at variable load amplitudes (in particular, under alternating loading modes), are determined. The presentation of corrected normal distribution laws with initial moments complements the normative documents used in practice when performing strength calculations under variable loading conditions, significantly simplifies calculations and makes it possible to take it into account at the stage of new design developments in accordance with the formulas established in the current normative documents.

Our ancestors: the Cimbri, Goths and Sarmatians. Three ethnogenetic legends in early modern Europe

ABSTRACT The study presented here is an attempt at a comparative analysis of three early modern phenomena in the history of ideas and culture: three ethnogenetic theories about the origins of the Swedish (Gothic) and Polish (Sarmatian) nations and states, as well as that of the Danes, originating from the ancient Cimbri. First, they will be compared as historiographic concepts characteristic of early modern knowledge. The next part of the paper concerns modifications to these theories made in national historiographies and the broader visions of the history of the respective countries based on them. The last part of the study presents how these ideas function at the political level, both in relation to broader visions of political reality and political practice. The analysis suggests that while in the case of the 16th-century historical concepts of origo gentis, there are more similarities than differences, the subsequent stages of development show increasing differentiation: the ideas of Sarmatian ethnogenesis underwent greater changes than others. However, there are similarities in the way Gothic and Sarmatian ideas functioned in political life and politics during that period in Sweden and Poland, respectively. The weakest point is the influence of Cimbrian ethnogenesis on the politics of Denmark at that time.

Toward Unrivaled Chromatographic Resolving Power in Proteomics: Design and Development of Comprehensive Spatial Three-Dimensional Liquid-Phase Separation Technology.

Spatial comprehensive three-dimensional chromatography (3D-LC) offers an innovative approach to achieve unprecedented resolving power in terms of peak capacity and sample throughput. This advanced technique separates components within a 3D separation space, where orthogonal retention mechanisms are incorporated. The parallel development of the second- and third-dimension stages effectively overcomes the inherent limitation of conventional multidimensional approaches, where sampled fractions are analyzed sequentially. This review focuses on the design aspects of the microchip for spatial 3D-LC and the selection of orthogonal separation modes to enable the analysis of intact proteins. The design considerations for the flow distributor and channel layout are discussed, along with various approaches to confine the flow during the subsequent development stages. Additionally, the integration of stationary phases into the microchip is addressed, and interfacing to mass spectrometry detection is discussed. According to Pareto optimality, the integration of isoelectric focusing, size-exclusion chromatography, and reversed-phase chromatography in a spatial 3D-LC approach is predicted to achieve an exceptional peak capacity of over 30,000 within a 1-h analysis, setting a new benchmark in chromatographic performance.

Ground subsidence and polygon development due to thermokarst in the Lena-Aldan interfluve, eastern Siberia, revealed by satellite remote sensing data

Thermokarst development is a topographic change in the landscape that is commonly associated with permafrost degradation in ice-rich permafrost regions. The Lena-Aldan interfluvial area in Central Yakutia in eastern Siberia has undergone extensive thermokarst development in the last three decades, particularly in the vicinity of settlements. Despite the negative effects of thermokarst development on the inhabitants of these settlements, no quantitative observation methods have been developed to investigate the surface displacement due to thermokarst development over entire towns. This study utilized interferometric synthetic aperture radar to reveal ground-surface displacement associated with thermokarst near the settlements of selected towns. The findings showed that significant subsidence was detected in disturbed areas (farming and abundant arable land) near the towns. The magnitude of subsidence in the Tyungyulyu and Mayya areas was less than that in Churapcha and Amga. Polygon density in a defined area in each town was examined using high-resolution optical images. The polygon density in Churapcha was considerably lower than that in Mayya, whereas polygonal texture in some areas in Tyungyulyu and Amga was unclear. Spatial frequency analysis using satellite optical images showed clear differences in averaged spectrum models between well-developed and less-developed polygons, which may reflect trough depths and density of vegetation between polygons. Satellite-based subsidence maps and statistics describing polygon development may be useful for evaluating both initial and subsequent stages of thermokarst development.

Twórczy rodzic - twórcze dziecko? Znaczenie środowisk wychowawczych w rozwoju potencjału twórczego dziecka

Aim: Educational environments heavily emphasize the well-rounded development of a child, often disregarding their individual needs. Schools and their teachers increasingly offer a wide range of "educational services," including elaborate interest-based clubs, skill-enhancement programs, and extracurricular activities. Children immersed in the pressure of "being the best at everything" often lose their natural curiosity about the world and are denied the opportunity to reflect on their true interests, leading to constant exhaustion. Parents are the primary caregivers and educators for their children, with the right and responsibility to raise them. In the process of upbringing, parents should not forget that a child has not only obligations but also rights. Often, in their desire to provide their children with material comforts, parents overlook the importance of being present in their everyday lives. Objective: To identify and present areas of interest for students attending additional classes, as well as to analyze the types of actions taken by significant people for children to develop their creative potential. Methods: diagnostic survey Results: According to the results of the diagnostic survey, active participation in the additional classes offered by schools has a positive impact on children’s development. Parents (80%) enroll their children in these classes, recognizing the need for such activities, while teachers see the need to reduce differences in compensatory classes. Additional classes help students develop their passions and achieve positive effects in physical, intellectual, cognitive, socio-moral, and emotional development. They also improve their memory and learn self-discipline. Significant adults confirm the beneficial impact of children’s participation in all areas of child development, emphasizing that it also translates into their child’s extracurricular functioning. Conclusion: Entities that participate in a child’s understanding of their immediate environment and the outside world have the task of not only delegating tasks to be performed or meeting their expectations but above all, participating and supporting the child in this effort. Both parents, as the first teachers, and teachers at subsequent stages of development, should play a special role as companions, inspirers, and observers of children’s development. Working with a child should be an opening to their subjectivity, individuality, and natural desire to gain new experiences. Originality: This is a scientific article presenting the results of original empirical research. Keywords. child, parent, teacher, development, educational environments, potential, creative child.

The evolution and transformation of youth entrepreneurship in China: a sociological analysis

The purpose of the article is to conduct a socio-historical study of the evolution and stages of the formation of youth entrepreneurship in China. The main problem is to identify and characterize the evolution and patterns that reveal the entrepreneurship of Chinese youth as a market mechanism that successfully functions in the conditions of the socialist economy of China. The object of the study is the entrepreneurial activity of young Chinese people. The subject of the study is the systematization of the stages of formation of youth entrepreneurship in various periods of transformation of Chinese society. The sources of information are scientific research carried out by Russian and Chinese researchers, as well as researchers from other countries. A comprehensive analysis of the sociodynamics of the development of youth entrepreneurship in China allows us to thoroughly and objectively identify and characterize the main systemic connections of this social phenomenon. The research methodology assumes a socio-historical analysis of the stages of the formation of youth entrepreneurship in China. A generalized analysis of the significant dependence of youth entrepreneurship on the general trends in the development of entrepreneurship in China as a social institution and the need for significant regulatory, organizational, educational efforts on the part of society and the state for its formation, maintenance and development is carried out. It is shown that at the stage of transition of Chinese society from subsistence farming to a planned economy (1945-1950), youth entrepreneurship was mainly spontaneous, non-institutional in nature and manifested itself as facts of entrepreneurial activity of individuals. The patriarchal way of life and rigid hierarchies of relations did not contribute to the development of entrepreneurial initiative of young people. At the second stage, in the process of transition to an industrial society (1951-1966), the centralization of the economy is increasing, and the main areas of activity of Chinese youth are directed to the political sphere, to the detriment of entrepreneurial initiatives in the economy. The third stage, the transition to an open society (1967-1978), is characterized by changes in the understanding of the political role of youth in society, the era after the Cultural Revolution laid the foundation for recognizing the important role of market foundations and youth entrepreneurship in the subsequent stages of China's development. The transition to a legal society (the fourth stage of the "Four Modernizations", after 1978), gives impetus to the development of youth entrepreneurship in China.