Subsequent Counts Research Articles

Post-exchange neutrophil count, but not post-hematocrit, predicts endogenous erythropoiesis in patients with sickle cell disease undergoing chronic red cell exchange.

With chronic transfusion in sickle cell disease (SCD), equipoise exists regarding whether increasing the post-procedure hematocrit (Hct) suppresses endogenous erythropoiesis. Reticulocytosis predicts SCD morbidity and mortality, so this study's objective was to clarify the role of the post-procedure Hct in suppressing reticulocytosis and to identify other potential red cell exchange (RCE) parameters predictive of reticulocytosis. This retrospective analysis of 17 patients who underwent chronic RCE at a single institution between 2014 and 2022 examined both standard red cell exchanges (SRCE) and exchanges preceded by isovolemic hemodilution (IVH-RCE). Post-procedure parameters with biologic plausibility to influence the subsequent procedure's absolute reticulocyte count (sPre-ARC) were examined using regression modeling. Neither post-hematocrit, nor post-hemoglobin (Hb), nor ΔHb/day was associated with sPre-ARC or the change in HbS% per day (ΔHbS%/day). Concurrent Hb was predictive for SRCE but not IVH-RCE, where ARC trended lower than with SRCE. Male gender and post-procedure neutrophil and white cell counts were predictors of sPre-ARC, consistent with their associations with SCD morbidity and mortality. IVH-RCE had a stronger correlation than standard RCE between pre-Hct and neutrophil or white cell depletion. Although targeting a post-procedure Hct maintains a higher subsequent pre-procedure Hb and a lower sPre-HbS%, it does not lead to sustained suppression of reticulocytosis as measured by the sPre-ARC or the ΔHbS%/day. IVH-RCE or the addition of hydroxyurea could be considered in those patients with high reticulocyte, white blood cell, or neutrophil counts.

Impact of 10% Dose Reductions and Duration of Treatment Delays in the Management of Chemotherapy-Induced Neutropenia in Dogs Treated With Common Chemotherapy Protocols: A Single-Centre Experience.

Neutropenia is a common chemotherapy-associated adverse event (AE) in dogs and a significant cause of decreased relative dose intensity. Dose reductions (DRs) and treatment delays (TDs) are frequently applied to decrease the risk of further neutropenic events (NEs) and AEs, but there is no standardised approach. The two main objectives of this retrospective study were to determine: (1) the failure rate of a 10% DR to prevent a subsequent inadequate absolute neutrophil count (ANC), defined as a nadir ANC <0.75 × 109/L or pretreatment ANC <1.5 × 109/L; and (2) if the duration of TDs due to pretreatment neutropenia affects the occurrence of subsequent NEs. A total of 1056 chemotherapy treatments were recorded for 128 dogs that developed at least one NE. In 75 of 124 (60.5%, 95% CI: 51.2%-69%) evaluable NEs, a nadir ANC of ≥0.75 × 109/L and pretreatment ANC of ≥1.5 × 109/L were achieved after a single 10% chemotherapy DR, while a 10% DR failed to prevent a subsequent inadequate ANC in the remaining 49/124 (39.5%, 95% CI: 30.1%-48.3%). The only variable associated with failure was the drug prescribed. DR failure occurred in 22/39 (56.4%, 95% CI: 40.9%-70.6%) lomustine DRs, 14/27 (51.9%, 95% CI: 33.9%-69.2%) cyclophosphamide DRs, but only 2/22 (9.1%, 95% CI: 2.5%-27.8%) doxorubicin DRs and 2/24 (8.3%, 95% CI: 2.3%-25.8%) vincristine DRs. Seventy-three evaluable TDs (mean: 5 days, SD ± 2.2 days) were prescribed. There was no association between TD duration and subsequent NEs (p = 0.11).

Dengue viremia kinetics and effects on platelet count and clinical outcomes: An analysis of 2340 patients from Vietnam.

Viremia is a critical factor in understanding the pathogenesis of dengue infection, but limited data exist on viremia kinetics. This study aimed to investigate the kinetics of viremia and its effects on subsequent platelet count, severe dengue, and plasma leakage. We pooled data from three studies conducted in Vietnam between 2000 and 2016, involving 2340 dengue patients with daily viremia measurements and platelet counts after symptom onset. Viremia kinetics were assessed using a random effects model that accounted for left-censored data. The effects of viremia on subsequent platelet count and clinical outcomes were examined using a landmark approach with a random effects model and logistic regression model with generalized estimating equations, respectively. The rate of viremia decline was derived from the model of viremia kinetics. Its effect on the clinical outcomes was assessed by logistic regression models. Viremia levels rapidly decreased following symptom onset, with variations observed depending on the infecting serotype. DENV-1 exhibited the highest mean viremia levels during the first 5-6 days, while DENV-4 demonstrated the shortest clearance time. Higher viremia levels were associated with decreased subsequent platelet counts from day 6 onwards. Elevated viremia levels on each illness day increased the risk of developing severe dengue and plasma leakage. However, the effect size decreased with later illness days. A more rapid decline in viremia is associated with a reduced risk of the clinical outcomes. This study provides comprehensive insights into viremia kinetics and its effect on subsequent platelet count and clinical outcomes in dengue patients. Our findings underscore the importance of measuring viremia levels during the early febrile phase for dengue studies and support the use of viremia kinetics as outcome for phase-2 dengue therapeutic trials. Wellcome Trust and European Union Seventh Framework Programme.

Epidemiology of Subsequent and Recurrent Injuries in Rugby: A Systematic Review

Objective To review and collate epidemiological data on recurrent and subsequent injuries in male rugby and identify risk factors such as injury type. Methods Four databases were searched from January 1, 1974 through October 1, 2020. Keywords were:((“Football”[Mesh]) OR (rugby)) AND ((recurrent injury) OR (subsequent injury)) in PubMed, (‘rugby’/exp OR rugby) AND (recurrent AND (‘injury’/exp OR injury) OR subsequent) AND (‘injury’/exp OR injury) in Embase, rugby AND (subsequent injury OR recurrent injury) in CINAHL Plus, and TOPIC: (rugby) AND TOPIC: (subsequent injur* OR recurrent injur*) in Web of Science. Inclusion Criteria:(i) population consisted of male rugby players; (ii) used “time-loss” as an injury definition; (iii) included extractable data on both subsequent injury count and recurrent injury count; (iv) written in English. 6/1242 studies met criteria for inclusion. Quality was assessed using STROBE guidelines. Results Studies using “missed-match” as a definition of time-loss showed no difference in incidence of subsequent versus recurrent injuries. Studies using “24-hour time-loss” as the definition of injury reported 7,724 (71%) subsequent injuries (95% CI: 0.71–0.72) and 1,434 (14%) recurrent injuries (95% CI: 0.13–0.15). In pediatric players, 98 (23%) were subsequent (95% CI: 0.19–0.27) and 21 (5%) were recurrent (95% CI: 0.03–0.08). When specifying concussions as an index injury, 119 (40%; 95% CI: 0.34–0.46) subsequent injuries other than a concussion were reported with 27 (9%; 95% CI: 0.06–0.13) recurrent concussions. Conclusion Injury definition and type of index injury plays a significant role when considering subsequent and recurrent injury incidence in male rugby players. Further investigation regarding incidence of secondary injury after specific types of index injury is warranted.

Quantifying the Effects of Energy Infrastructure on Bird Populations and Biodiversity.

Shale oil and gas production and wind energy generation both expanded rapidly across the United States between 2000 and 2020, raising concerns over impacts on wildlife. I combine longitudinal microdata from the National Audubon Society's Christmas Bird Count with geolocated registries of all wind turbines and shale wells constructed in the contiguous U.S. during this period to estimate the causal effects of these contrasting types of energy infrastructure on bird populations and biodiversity, which are key bellwethers of ecosystem health. Results show that the onset of shale oil and gas production reduces subsequent bird population counts by 15%, even after adjusting for location and year fixed effects, weather, counting effort, and land-use changes. Wind turbines do not have any measurable impact on bird counts. Negative effects of shale are larger when wells are drilled within important bird habitats.

Radiation-Associated Lymphopenia in Advanced Prostate Cancer Treated with Contemporary Radiation Techniques.

Lymphocytes play a critical role in the immune system as primary effector cells for cancer control, often depleted by external beam radiation therapy (EBRT). Radiation-associated lymphopenia (RAL) has been shown to be a poor prognostic factor in the management of multiple solid tumors. We hypothesize RAL is similarly observed in advanced prostate cancer (PC) RT with contemporary techniques. We identified patients with advanced PC (high-risk or clinical/pathologic node-positive) receiving EBRT including lymph node/prostatic lesion boost on a prospective collection protocol for whom 1 baseline and ≥2 subsequent complete blood count (CBC) with differential samples were available, collected at RT end, 3-, 6-, and 12-months post-RT. Clinicopathological characteristics were retrieved from chart review. Common Terminology Criteria for Adverse Events (CTCAE)v5 was used to grade absolute lymphocyte count (ALC); RAL was defined as CTCAEv5 grade ≥2. As these patients received pelvic nodal irradiation, they were pooled with low/intermediate-risk PC cohort treated with high dose-rate (HDR) brachytherapy or prostate alone EBRT with similar CBC timepoints for univariable analysis to understand RT field size effect on RAL. Between 2019 and 2022, among 17 patients in the low/intermediate-risk PC cohort, 6 patients had grade ≥2 lymphopenia. Among 25 patients in the advanced PC cohort, all received androgen deprivation therapy (ADT), 6 received lymph node boost, and 5 received prostatic lesion boost. At RT end, leukopenia was prominently observed (median nadir count 75.1% of baseline), with ALC as major driver (median nadir count 27.3% of baseline). Grade ≥2 lymphopenia was observed in 76% of patients (n = 19) Of 19 advanced PC patients who reached 6 months post-RT follow-up, median ALC was 53.0% of baseline, and Grade ≥2 lymphopenia remained in 37% (n = 7) of patients. Of 8 advanced PC patients who reached 12 months post-RT follow-up, median ALC was 55.6% of baseline. When evaluating whether RT dose or field size contributed to lower nadir ALC counts, combining the low/intermediate-risk and advanced PC cohorts (n = 42), univariable analysis demonstrated Gleason grade group (p = 0.009), RT field size (p = 0.020), ADT use (p = 0.020), baseline ALC (p = 0.037), and baseline hemoglobin (p = 0.009) were independent predictors of Grade ≥2 lymphopenia. Age, prostatic lesion/lymph node boost, and equivalent dose in 2 Gy/fraction (EQD2) were nonsignificant. Grade ≥2 RAL was observed in patients with advanced PC at end of RT, irrespective of age, RT boost, or EQD2. Lymphocyte recovery from baseline can be prolonged even at 12 months post-RT. Ongoing analyses include expanding data with additional serial CBC, increasing cohort size, and integrating effect of additional systemic therapies. RAL has downstream implications for future chemotherapy/radiopharmaceuticals.

Citation pattern of open access and toll-based research articles in the field of biological and physical sciences: a comparative study

PurposeThe purpose of this paper is to determine the relationship between the access mode of research articles [Open Access (OA) and Toll-Access (TA)] and their subsequent citation counts in Biological and Physical Sciences in three Impact factor zones (High, Medium and Low).Design/methodology/approachThree subjects each from Biological Sciences (Biochemistry, Cell Biology and Genetics) and Physical Sciences (Astronomy, Oceanography and Optics) were selected for the study. A comprehensive list of journals (TA and OA) in select subjects of Biological and Physical Sciences was prepared by consulting Journal Citation Report’s Master Journal List (for the compilation of both Open Access and Toll Access journal list) and Directory of Open Access Journals (for the compilation of Open Access journal list). For each journal, essential details like content language, format, year of publication, access mode (Open Access or Toll Access), etc. were obtained from Ulrich’s Periodical Directory. Web of Science (WoS) was used as citations indexing tool in this study. The data set was run on the WoS to collect the citation data.FindingsThe results of the study indicate that open mode of access is not a prerequisite for higher citation boost as in the majority of the cases in this study, TA articles have garnered a greater number of citations as compared to open access articles in different Impact factor zones in Biological and Physical Sciences.Originality/valueA novel approach has been adopted to understand and compare the research impact of open access (OA) and toll access (TA) journal articles in the field of Biological and Physical Sciences at three Impact factor zone levels to reveal the citation metrics encompassing three parameters, i.e. citedness, average citation count and year wise distribution of citations in select subjects of Biological and Physical Sciences.Peer reviewThe peer review history for this article is available at: https://publons.com/publon/[DOI]/10.1108/OIR-01-2021-0029

Antinuclear antibodies in childhood immune thrombocytopaenia: An increased risk for development of autoimmune disorders?

Antinuclear antibodies (ANA) have been detected in children with primary immune thrombocytopaenia (ITP), but the effect of ANA titres on clinical outcomes is unclear. Liu et al. retrospectively analysed a cohort of 324 children with primary ITP with a median follow-up time of 25 months and found that patients with high-ANA titres (≥1:160) had lower platelet counts at the onset with a higher subsequent platelet count recovery rate, and additionally were at an increased risk to develop autoimmune disease. These data highlight the possible predictive potential of ANA titres with respect to platelet counts and the development of autoimmunity in children with primary ITP. Commentary on: Liu, et al. The effect of antinuclear antibody titre and its variation on outcomes in children with primary immune thrombocytopaenia. Br J Haematol 2023 (Online ahead of print). doi: 10.1111/bjh.18732.

The effect of antinuclear antibody titre and its variation on outcomes in children with primary immune thrombocytopenia.

Antinuclear antibody (ANA) can be positive in children with primary immune thrombocytopenia (ITP), but the effect of ANA titre and its variation on outcomes of children with primary ITP remains unclear. Here, we conducted a single-centre retrospective cohort study of children with primary ITP at the Peking Union Medical College Hospital in China. A total of 324 children with primary ITP included in this study were followed for a median time of 25 months. In this cohort, 39.2% had an ANA titre of 1:160 or higher. Results from a generalized estimating equation model revealed that patients with higher ANA titres had lower platelet counts at onset but a higher recovery rate of subsequent platelet counts. Results from Cox regression models adjusted for potential confounders revealed that patients with ANA titres of 1:160 or more were more likely to develop to autoimmune disease (AID) than those without, and the risk of AID development increased with the rise of ANA titres (p value for trend less than 0.001). These data highlight the predictive value of ANA titre for platelet counts and the risk of AID development in children with primary ITP.

Laser Intensity of Thermo-Responsive Nanoparticles Size Measurement Using Dynamic Light Scattering

Dynamic light scattering (DLS) or photon cross-correlation spectroscopy (PCCS) is a very potent means for analyzing the diffusion behaviour of supramolecules in their solution form. The diffusion coefficient, probed from the scattered light by supramolecules underexposure to incident light, allows the hydrodynamic radii to be calculated. However, the scattering values were recently misled by an unexpected interaction between the incident light and thermo-sensitive nanogels. Hence, in turn, it resulted in a miscalculation of the size of the nanogels by more than 100% of their expected values. The study fulfills a vital knowledge gap by investigating the effects of laser intensity on the size of thermo-sensitive Poly(N-Isopropyl Acrylamide-Vinyl Pyrrolidone-Polyethylene Glycol Diacrylate-(2-(Dimethyl Amino)Ethyl Methacrylate)), Poly(NIPAAM-PVP-PEGDA-DMAEMA) nanogels and subsequent count rates in DLS measurements. The Lower Critical Solution Temperature, LCST phase transition of Poly(NIPAAM-PVP-PEGDA-DMAEMA) nanogel can be observed using the DLS technique. The higher laser intensity was advantageous for measuring at high dilution more vigorously with varying laser intensities. Thus, a sufficient laser intensity should be chosen based on the light scattering characteristics of typical samples.

The Effect Of Neutropenia On The Delivery Of Chemotherapy In Breast Cancer Patients At Korle Bu Teaching Hospital In Accra, Ghana

Introduction: Treatment of breast cancer involves chemotherapy, of which the commonest complication is neutropenia and febrile neutropenia. Neutropenic events interrupt the administration of effective chemotherapy and results in poorer outcomes. This study evaluates the impact of neutropenia on the effective delivery of anthracycline-based chemotherapy in breast cancer patients and identifies those at risk of neutropenic events.Methods: This is a prospective study of one hundred and ninety patients presenting with breast cancer who received both adjuvant and neoadjuvant chemotherapy from January 2013 to July 2014. Patients received cyclophosphamide, doxorubicin and 5-fluorouracil.Data collected included baseline absolute neutrophil count and subsequent absolute neutrophil counts on day 10 and day 20 throughout the course of therapy to identify any neutropaenic events. Univariate logistic regression analysis of age, body surface area and ECOG performance was done to determine factors associated with neutropaenia.Results: The prevalence of neutropenia was 8.7% and that of febrile neutropenia was 0.24%. Body surface area of &lt; 2 m2 and first cycle neutropenia were found to be associated with an increased risk of developing neutropenia.Conclusion: The prevalence of neutropaenia and febrile neutropaenia in this study was relatively low 8.7% and 0.24% respectively. The only factor found to be associated with neutropenic events is a BSA&lt; 2m2. This study forms a basis for a larger study aimed at identifying predictors and developing a model to predict neutropenia risk in the Ghanaian Population.

Abstract A027: Exploring the effect of hypoxia and spatial interactions on the dynamics between gefitinib resistant and naïve NSCLC cell lines

Abstract Hypoxia and the development of spatially heterogeneous hypoxic and/or necrotic regions in non-small cell lung cancer (NSCLC) and other solid tumors is associated with chemotherapy and radiation resistance. One mechanism by which this could occur is through modification of the cell-cell interactions (or games) between the resistant and naïve cells within the tumor. Cell-cell interactions can modify the fitness of cells and thereby change the evolutionary dynamics of a tumor. Hypoxia may, in this way, change the game dynamics between cells and directly support maintenance of a population of resistant cells. To understand the mechanism by which hypoxic heterogeneity modulates evolutionary dynamics and therapy response, we combine computational modeling and in vitro experiments under a spatial hypoxia gradient. We utilize in-house game assay protocols to probe the evolutionary games between cell types. The experimental system is adapted from Carmona-Fontaine's MEMIC plate. This system allows us to create stable oxygen and nutrient gradients for 12 evolutionary replicates of NSCLC PC-9 cells. Fluorescence labeled gefitinib-resistant PC-9 cells and naïve parental PC-9 cells are co-cultured in a closed chamber with limited availability to oxygen and nutrient supply at one end. A green hypoxia probe is used to verify the establishment of a hypoxia gradient and a red Annexin V probe monitors apoptosis. Once microenvironmental gradients are established due to cellular consumption of oxygen and nutrients, images of cells are taken every 1.5 hours for 5 days. We carry out multiple experiments in which we vary the initial proportions of parental and resistant cells. Computationally, green and red fluorescence from the images captured by the microscopy system is quantified by an in-house image processing pipeline. We apply a random forest machine learning classifier, illumination correction, and object identification to each image to gather fluorescence counts for each well. These counts are used to compute the background rate of cell death and absolute vs. relative oxygen levels. The subsequent cell counts over time were used to calculate the growth rate (fitness) of the cell types in different environments and therefore the strength of environmental and frequency dependent interactions between the cell types. This framework allows for tightly coupled computational and in vitro experiments aimed at understanding and predicting the clinical implications of hypoxia resistance across a range of tumor types and microenvironments. We have measured the effect of hypoxia upon game interactions between gefinib-resistant and susceptible NSCLC cells. The evolutionary game changes with changing levels of hypoxia–and for the first time we are able to write down a continuous equation representing the game as a function of oxygen. Citation Format: Mina Dinh, Bezhou Feng, Jeff Maltas, Emily Dolson, Masahiro Hitomi, Steph Owen, Jacob Scott. Exploring the effect of hypoxia and spatial interactions on the dynamics between gefitinib resistant and naïve NSCLC cell lines [abstract]. In: Proceedings of the AACR Special Conference on the Evolutionary Dynamics in Carcinogenesis and Response to Therapy; 2022 Mar 14-17. Philadelphia (PA): AACR; Cancer Res 2022;82(10 Suppl):Abstract nr A027.

Serotonin Surge: Intravenous Escitalopram as a Rare Cause of Drug-Induced Aplastic Anaemia.

Escitalopram is a commonly prescribed medication that has infrequently been implicated in drug-induced thrombocytopenia (DITP) but has never been associated with aplastic anaemia in the literature. We present an extremely rare case of hypoproliferative pancytopenia due to self-administered intravenous (IV) injection of escitalopram. The crux of this case is the unusual trilineage cytopenia. Our patient was managed with steroids and supportive care with subsequent clinical and blood count recovery. This case sheds light on this uncommon but important association.LEARNING POINTSEscitalopram is an uncommon medication that could lead to drug-induced aplastic anaemia.The adverse effects of escitalopram on red cell, white cell and platelet counts may be exacerbated on intravenous administration of the medication.Timely diagnosis is vital for the effective treatment of severe aplastic anaemia, avoiding complications and preventing recurrence.

William (Bill) Richmond Postle Bourne, MA, MB, B CH, MBOU (1930–2021)

William (Bill) Richmond Postle Bourne, MA, MB, B CH, MBOU (1930–2021)

Abstract P047: Depressive Symptom Trajectory Associated With Step Counts From Smartwatch: The Electronic Framingham Heart Study

Introduction: Depression is a common and serious medical illness world-wide. Studies have demonstrated a protective effect of physical activity on depression, but few studies have examined the prospective association between depressive symptoms and objectively measured physical activity. Objective: To investigate if antecedent depressive symptoms predict subsequent daily step counts among participants enrolled in the electronic Framingham Heart Study (eFHS). We hypothesize that the trajectory group with a larger proportion of participants having depressive symptoms and antidepressant use is associated with a lower level of physical activity. Methods: We used group-based multi-trajectory modeling to construct depressive symptom trajectory patterns using both depressive symptoms and antidepressant use collected from eFHS participants who attended three FHS health exams over fourteen years. The presence of depressive symptoms was defined at each exam with a Center for Epidemiological Studies-Depression (CES-D) score &gt; 16. At the final exam, participants were provided with a study smartwatch to measure daily step counts. We excluded: a) person-day records with less than 5 hours of wear-time, or less than 1000 steps recorded in a day; b) participants who failed to return data for ≥1 day per week in the first month. We performed linear mixed effects models to examine the association between depressive symptom trajectories and average daily step counts during the first 30 days of smartwatch use and one-year of follow-up adjusting for age, sex, wear-hour, BMI, and smoking status. We used p&lt;0.05 for significance. Results: We identified two depressive symptom trajectory groups for the 724 eFHS participants (mean age 53 years, 60% women). The “low-stable” group (n=578; mean follow-up 286±110 days) consisted of &lt;5% of participants with depressive symptoms and &lt;3% of those with antidepressant medication use across the three exams. The “high-increasing” group ( n = 146; 269±114 days) consisted of &gt;21% of participants with depressive symptoms and &gt;46% of those with antidepressant medication use across the three exams. Compared to the low-stable trajectory group, the participants in the high-increasing depressive symptoms group walked 475 (95% CI: 24-927) fewer daily steps during the first 30 days of smartwatch use ( p = 0.04) and walked 550 (95% CI: 109-991) fewer daily steps during one-year of follow-up ( p = 0.01). Conclusion: Participants in the group with a larger proportion of antecedent depression and anti-depressant use walked significantly fewer steps compared to the group with a smaller proportion of antecedent depression and anti-depressant use. Our findings suggest that interventions via mHealth technologies that target mood may play an important role in promoting physical activity.

Naturally-Occurring Radioactive Materials at water treatment plant on the Poços de Caldas Plateau Region, Brazil

ABSTRACT Oil and gas, mining, among others, are examples of facilities where naturally occurring radioactive materials can be found. This study aims to evaluate the presence of natural radioactive series, especially those of 238U and 232Th, in the water treatment plants of Poços de Caldas City, Minas Gerais. The presence of these series was investigated in samples of raw water, treated water, sludge from decanters, and scale from Parshall gutters. The sludge, input, and scale samples were submitted to the gamma spectrometry technique to determine the 226Ra, 228Ra, and 210Pb radionuclides. For U and Th, ultraviolet visible spectrophotometry was performed, and for the alpha and beta total values, radiochemical separation and subsequent alpha and beta total counts were performed. The results indicate that water samples are within the Ministry of Health Ordinance n° 5 (2017). Due to the different concentrations of radionuclide activity in the sludge, it was not possible to affirm the same order of magnitude with the sediment from the catchments. However, the values are in accordance with those established by the European Union Council for Naturally-Occurring Radioactive Materials. In the scale, the contents of 1192, 1704, and 301 Bq kg−1 were identified for 226Ra, 228Ra, and 210Pb, respectively. In the inputs of aluminum sulfate and calcium hydroxide, no relevant activities were identified. The results obtained in the study can serve as an indicative regarding the need for a more detailed evaluation of the radiological issue in question concerning public water supplies.

Landscape-species national inventory of animal habitats as a basis for hunting resources use and protection (on the sable example)

The purpose of our message is to characterize the principles used in national inventory development and to show possibilities of landscape-species approach for characterizing hunting resources distribution. This represents a certain scientific novelty. Quantitative data collection is based on the use of partial shooting (catching) method when mapping the results of cropping in the areas of commercial hunting areas. Cadastral developments are related to the quality of animals wildlife habitual area. On Irkutsk region territory, four regional complexes of sable habitats have been identified: Angarsk, Verkholensk and South Prebaikalsk, and Khamar-Daban. The basis for their identification was participation proportion in Siberian cedar composition for the first two and the presence of dwarf pine overgrowth for Khamar-Daban along with Verkholensk eastern part. The South CisBaikal complex of habitats, including Eastern Sayan habitats, is characterized primarily by the absence of dwarf pine overgrowth. The proposed methodological system for the inventory and assessment of game animals habitats is based on the ecological interpretation of landscape units (groups of geographic eco-elements). It makes it possible to take into account the regional characteristics of animal habitat and allows forecasting the dynamics of habitats. For the territory characterized by mapping on the principles of structural-dynamic landscape science, it is necessary to fully use the cartographic landscape basis on these principles. It is quite enough to select the best (optimal) habitats that provide animals with living conditions throughout the entire annual life cycle, and to protect them accordingly, as the basis for maintaining population homeostasis within the region; suboptimal - much worse, used mainly seasonally; and hunting areas are not peculiar to the species of animals, on which the species does not live and cannot live. The first two categories of habitats during mapping will make up the lace of the species range within the region, and all for a total, the habitats species national inventory, and at the same time strata for collecting initial data and extrapolating during counts. Thus, cadastral developments with a landscape- species approach make it possible to stratify the territory for subsequent counts of animals number of a certain species. This is especially important for economically significant species, which number state must be accurately tracked for their rational use.

Hepatosplenomegaly and Periventricular Cyst in a Neonate with Direct Hyperbilirubinemia

Hepatosplenomegaly and Periventricular Cyst in a Neonate with Direct Hyperbilirubinemia

Cold-stored whole blood and platelet counts in severe acute injury: A comparison of four retrospective cohorts.

Low-titer group O whole blood (LTOWB) is attractive for acute trauma care as it delivers concentrated and balanced hemostatic resuscitation in single large bags. Whether cold-stored LTOWB can sustain platelet counts is unclear. Four cohorts of trauma patients-three historic, one retrospective-were identified by their urgency of blood use. Admission and all subsequent platelet counts over the first 24 h of care were compared with t-tests. The cohorts were as follows: 1292 patients at Maryland Shock Trauma as described by Stansbury and colleagues in 2013; 35 patients enrolled locally in the 1:1:2 arm of the pragmatic randomized optimal plasma and platelet ratios (PROPPR) trial; 34 patients enrolled locally in the 1:1:1 arm of PROPPR; and 59 patients receiving more than 3units of LTOWB enroute to or at our Level 1 trauma center, 2019-2020. Mean age of LTOWB units transfused was 9± 5 days and mean dose was 5± 2units. All four cohorts were profoundly injured (mean Injury Severity Score ≥ 31), with mean first platelet counts 204-228 K/μ and subsequent counts approximately 100 k/μl lower. Through the first 24 h of care, mean platelet counts decreased least, 79 and 83 103 /μl, in the 1:1:1 PROPPR and LTOWB cohorts. Mean platelet counts in patients transfused with LTOWB remained stable after the third hour of care. LTOWB transfusion was associated with lesser mean decrease in platelet counts during the first 24 h after injury, similar to those observed among patients receiving components 1:1:1 component in the PROPPR study.

Efficiency of ultraviolet light for disinfection of fertile broiler eggs

ABSTRACT This study aimed to evaluate the effectiveness of ultraviolet light in reducing bacterial load of eggshells and the impact of experimental disinfection on hatching, embryo mortality, and time-borne distribution using broiler breeder hens of different ages (38, 42, and 48 weeks old). Fertile eggs were subjected to different exposure periods (5, 7, and 9 minutes) of UV light (UV-C) with a 254 nm wavelength. For controls, eggs disinfected with paraformaldehyde (5.3 g/m3) and eggs not disinfected (NC). After subjection to disinfection protocols, the eggs were placed into sterile plastic bags containing 20 mL of peptone saline solution (0.1% m:v) and massaged for 1 minute to release the bacterial load. Aliquots of this solution were incubated in specific medium for bacterial growth for 48 hours at 37ºC for subsequent CFU counts. To evaluate the effects of disinfection on production, eggs previously disinfected by UV-C (9 min) and paraformaldehyde and NC eggs were candled between incubation days 10 and 13 and at the end of the incubation period to assess embryonic mortality. Hatchability distribution was performed every 8 hours. The 9 minutes 254nm UV-C light exposure was able to disinfect viable eggs and matched the effectiveness of the paraformaldehyde technique.