Subglacial Environments Research Articles

Methanogenic potential of Arctic and Antarctic subglacial environments with contrasting organic carbon sources

AbstractSubglacial environments are largely anoxic, contain organic carbon (OC) overridden by glacier ice during periods of advance, and harbour active microbial communities. This creates favourable conditions for OC degradation via methanogenesis. It has been hypothesized that OC beneath ice sheets is converted to methane (CH4) and may be released to the atmosphere during retreat. However, there are limited data available to support this contention. Here, we present new data on the abundance, diversity and activity of methanogenic archaea and the amount and character of OC in subglacial sediments from Arctic and Antarctic glacial systems based on different substrate types. We employed long‐term laboratory incubations to quantify the CH4 production potential in different subglacial settings. Significant numbers of methanogens (up to 7 × 104 cells g−1) were detected in the samples and clones of Methanomicrobiales and Methanosarcinales were identified in clone libraries. Long lag periods (up to >200 days) were observed before significant CH4 concentrations were measured. We report order of magnitude differences in rates of CH4 production (101–105 fmol g−1 d−1) in different subglacial sediments, reflecting contrasts in the origin of the sediment and the OC character. Hence, we predict that contrasting rates of CH4 production are likely to occur beneath glaciers and ice sheets that overran different types of substrate. We subsequently estimated the potential for CH4 production beneath the Laurentide/Inuitian/Cordilleran and Fennoscandian Ice Sheets during a typical 85 ka Quaternary glacial/interglacial cycle. CH4 production from lacustrine‐derived OC is likely to be an order of magnitude higher (~6.3–27 Pg C) than that from overridden soils (~0.55–0.68 Pg C), possibly due to a difference in lability between lacustrine and soil OC. While representing a fraction of the entire OC pool (~418–610 Pg C), this finding highlights the importance of considering the character of different OC pools when calculating subglacial CH4 production.

Microstructural analyses of a Middle Pliocene till from the James Bay Lowlands, Canada—evidence of “potential” fast ice streaming

Abstract Sediment from the Attawapiskat area near James Bay, Northern Ontario was sampled for micromorphological analyses. The sediment is a glacial diamicton (till) of subglacial origin. The till contains entrained and scavenged sediments of proglacial and/or subglacial glaciofluvial/glaciolacustrine origin from a subglacial deforming layer that was emplaced due to both stress reduction and/or porewater dissipation. Evidence of porewater escape, clay translocation and other microstructures all point to emplacement under active subglacial bed deformation. The limited number of edge to edge (ee) grain crushing events, however, point to lower stress levels than might anticipated under a thin fast ice lobe of the James Bay during the Middle Pliocene. Microstructures of Pleistocene tills were quantitatively compared with the Attawapiskat till and the limited number of ee events at Attawapiskat further highlighted that grain to grain contact was curtailed possibly due to high till porosity, high porewater pressures and low strain rates or alternatively due to a high clay matrix component reducing grain crushing contact events. It is suggested that this Middle Pliocene till may be indicative of sediments emplaced under ice lobe surging conditions or fast ice stream subglacial environments. This proposal has significant implications for the glaciodynamics of this part of the Middle Pliocene James Bay lobe. This research highlights a crucial link between subglacial conditions, till microstructural analyses and glaciodynamics.

Potential methane reservoirs beneath Antarctica

Once thought to be devoid of life, the ice-covered parts of Antarctica are now known to be a reservoir of metabolically active microbial cells and organic carbon. The potential for methanogenic archaea to support the degradation of organic carbon to methane beneath the ice, however, has not yet been evaluated. Large sedimentary basins containing marine sequences up to 14 kilometres thick and an estimated 21,000 petagrams (1 Pg equals 10(15) g) of organic carbon are buried beneath the Antarctic Ice Sheet. No data exist for rates of methanogenesis in sub-Antarctic marine sediments. Here we present experimental data from other subglacial environments that demonstrate the potential for overridden organic matter beneath glacial systems to produce methane. We also numerically simulate the accumulation of methane in Antarctic sedimentary basins using an established one-dimensional hydrate model and show that pressure/temperature conditions favour methane hydrate formation down to sediment depths of about 300 metres in West Antarctica and 700 metres in East Antarctica. Our results demonstrate the potential for methane hydrate accumulation in Antarctic sedimentary basins, where the total inventory depends on rates of organic carbon degradation and conditions at the ice-sheet bed. We calculate that the sub-Antarctic hydrate inventory could be of the same order of magnitude as that of recent estimates made for Arctic permafrost. Our findings suggest that the Antarctic Ice Sheet may be a neglected but important component of the global methane budget, with the potential to act as a positive feedback on climate warming during ice-sheet wastage.

A fourth inventory of Antarctic subglacial lakes

AbstractAntarctic subglacial lakes are studied for three main scientific reasons. First, they form an important component of the basal hydrological system which is known to affect the dynamics of the ice sheet. Second, they are amongst the most extreme viable habitats on Earth and third, if sediments exist on their floors, they may contain high-resolution records of ice sheet history. Here we present a new inventory of locations, dimensions and data sources for 379 subglacial lakes. Several major advances are responsible for the rise in the total number of lakes from the 145 known at the time of the last inventory in 2005. New radar datasets have been collected in previously unexplored regions of the ice sheet while digital data collection and processing techniques have allowed improvements to lake identification methods. Satellite measurements of ice surface elevation change caused by the movement of subglacial water have also been found to be widespread in Antarctica, often in places where radar data are absent. These advances have changed our appreciation of the Antarctic subglacial environment and have expanded our understanding of the behaviour of subglacial lakes.

Dissolved gases in frozen basal water from the NGRIP borehole: implications for biogeochemical processes beneath the Greenland Ice Sheet

Little information exists on biogeochemical transformations in aquatic ecosystems beneath polar ice sheets (i.e., water-saturated sediments, streams, rivers, and lakes) and their role in global elemental cycles. Subglacial environments may represent important sources of atmospheric CO2 and/or CH4 during deglaciation, thus acting as amplifiers in the climate system. However, the role of subglacial environments in global climate processes has been difficult to assess given the absence of biogeochemical data from the basal zones of inland polar ice sheets. Here, we report on the concentrations of CO2, CH4, and H2 in samples of refrozen basal water recovered at a depth of ~3,042 meters below the surface during the North Greenland Ice Core Project (NGRIP). CH4 and H2 concentrations in the NGRIP samples were approximately 60- and 700-fold higher, respectively, relative to air-equilibrated water, whereas CO2 was ~fivefold lower. Metabolic pathways such as (1) methanogenesis, (2) organic matter fermentation, carboxydotrophic, and/or methylotrophic activity, and (3) CO2 fixation provide plausible biotic explanations for the observed CH4, H2, and CO2 concentrations, respectively.

Glacial terrain zone analysis of a fragmented paleoglaciologic record, southeast Keewatin sector of the Laurentide Ice Sheet

A highly fragmented subglacial landscape is recognized at the regional to sub-regional scales in northeastern Manitoba, Canada, in the southeast Keewatin Sector (a core region) of the Laurentide Ice Sheet. New field-based ice-flow indicator measurements, mapping of subglacial landforms (remote-sensing and aerial photograph), and a re-examination of previously published data from an 8100km2 area in northeastern Manitoba show that the preserved subglacial record reflects a complex and potentially long glacial history. Five streamlined landform flowsets are mapped. A much higher degree of inheritance in the field-based ice-flow indicator data, than previously reported, allows for recognition of multiple ice-flow phases. Analysis of the characteristics of the subglacial landscape combined with a relative-age chronology established with field-based indicators, led to the recognition of disjoint zones with internally-consistent glacial histories – termed glacial terrain zones (GTZ). These GTZ were then classified as (1) relict glacial, (2) palimpsest, or (3) deglacial in nature.Our data suggest that while the southern Keewatin Sector was affected by regional ice-divide translocation, this alone cannot explain the fragmented, high inheritance landscape. We suggest that the subglacial landscape was continually evolving and subject to spatio-temporal variations in intensity of erosion, transportation and/or deposition throughout multiple glacial events (subglacial bed mosaic). Preservation of relict and palimpsest terrain likely occurred under large ‘sticky’ low-erosion regions. These regions could have formed by at least two different mechanisms: heterogeneous switch from warm-based to cold-based ice or within a warm-based subglacial environment from wet to stiff, dewatered till. Establishment of the regionally extensive (∼700km wide by at least 500km long) dendritic esker channel-system may have caused rapid spatially-variable dewatering of the substrate far back under the ice sheet. The GTZ approach integrates all available data (e.g. flowsets and other landform data, striations) to advance our interpretation of the spatio-temporal evolution of subglacial dynamics in areas where the degree of landscape inheritance and overprinting is spatially highly variable. This mosaic may be a characteristic net-effect of landscape evolution beneath the core regions of ice sheets.

In-Field Implementation of a Recombinant Factor C Assay for the Detection of Lipopolysaccharide as a Biomarker of Extant Life within Glacial Environments.

The discovery over the past two decades of viable microbial communities within glaciers has promoted interest in the role of glaciers and ice sheets (the cryosphere) as contributors to subglacial erosion, global biodiversity, and in regulating global biogeochemical cycles. In situ or in-field detection and characterisation of microbial communities is becoming recognised as an important approach to improve our understanding of such communities. Within this context we demonstrate, for the first time, the ability to detect Gram-negative bacteria in glacial field-environments (including subglacial environments) via the detection of lipopolysaccharide (LPS); an important component of Gram-negative bacterial cell walls. In-field measurements were performed using the recently commercialised PyroGene® recombinant Factor C (rFC) endotoxin detection system and used in conjunction with a handheld fluorometer to measure the fluorescent endpoint of the assay. Twenty-seven glacial samples were collected from the surface, bed and terminus of a low-biomass Arctic valley glacier (Engabreen, Northern Norway), and were analysed in a field laboratory using the rFC assay. Sixteen of these samples returned positive LPS detection. This work demonstrates that LPS detection via rFC assay is a viable in-field method and is expected to be a useful proxy for microbial cell concentrations in low biomass environments.

Depositional model in subglacial cavities, Killiney Bay, Ireland. Interactions between sedimentation, deformation and glacial dynamics

Subglacial meltwater drainage and sedimentary processes play a major role in ice-sheet dynamic but there is a lack of study of subglacial environment because modern ice-sheet beds remain inaccessible. Previous authors already intended to provide diagnostic criterion and recent investigations suggest that fluid pressure variations are a key factor in subglacial environment. This paper investigated the late Devensian sedimentary record in order to describe subglacial sedimentological facies associations and deformation features related to fluid overpressures. We used an integrated approach, based on stratigraphy, sedimentology and deformations styles to demonstrate a subglacial depositional model. The studied interval is composed of stratified gravel and sand interbedded with diamicton and boulder pavement, deposited in depressions formed by irregularity of the upper surface of diamicton. Deformation structures include convolutes, dykes and normal micro-faulting. Dykes show different dip directions from vertical, oblique to subhorizontal from which both directions of shortening and extension can be determined. Vertical dykes are formed under pure shear strain related to ice weight only. Oblique dykes imply both ice-bed coupling and simple shear strain between ice and substrate induced by flowing ice related to progressively increasing meltwater drainage intensity. Horizontal dykes are formed when minimum strain is vertical and therefore the overpressure exceeds the weight of overburden. They are associated with high meltwater drainage intensity and ice-bed uncoupling and refer to hydrofracturing. Overall, depositional and deformation sequence also illustrates the increasing intensity of meltwater drainage in small cavity as high energy channelised deposits, and in large cavities where subaqueous fan are deposited under hydraulic jump conditions. Moreover, large cavities represent lee-side cavities formed by fast-flowing ice over an obstacle. Hydrofracturing is likely to occur when a dense fluid, potentially associated with catastrophic drainage of an upstream cavity enters the low-pressure confined environment of a downstream cavity and is injected under pressure in the soft substrate. The studied glacial sequence represents a regional pattern probably related to an allocyclic control on sedimentation linked to long-term glacial dynamics rather than local depositional conditions. Based on these results, we provided a synthetic model linking depositional and deformation processes during ice-sheet growth and decay, but also valid at different timescales from annual to seasonal scale.

Clean access, measurement, and sampling of Ellsworth Subglacial Lake: A method for exploring deep Antarctic subglacial lake environments

Antarctic subglacial lakes are thought to be extreme habitats for microbial life and may contain important records of ice sheet history and climate change within their lake floor sediments. To find whether or not this is true, and to answer the science questions that would follow, direct measurement and sampling of these environments are required. Ever since the water depth of Vostok Subglacial Lake was shown to be >500 m, attention has been given to how these unique, ancient, and pristine environments may be entered without contamination and adverse disturbance. Several organizations have offered guidelines on the desirable cleanliness and sterility requirements for direct sampling experiments, including the U.S. National Academy of Sciences and the Scientific Committee on Antarctic Research. Here we summarize the scientific protocols and methods being developed for the exploration of Ellsworth Subglacial Lake in West Antarctica, planned for 2012–2013, which we offer as a guide to future subglacial environment research missions. The proposed exploration involves accessing the lake using a hot‐water drill and deploying a sampling probe and sediment corer to allow sample collection. We focus here on how this can be undertaken with minimal environmental impact while maximizing scientific return without compromising the environment for future experiments.

A buried glaciofluvial channel in the Anděl Col, Northern Bohemia: new evidence for the Middle Pleistocene ice sheets extent in Western Sudetes

This study presents a new finding of glaciofluvial sediments filling a buried channel in the Anděl Col (522 m a.s.l.) in the Jizera Mts., on the Czech-Polish border. At least 550 m long and 55–100 m wide channel filled by glaciofluvial sediments was determined by geophysical methods in the lowermost part of the col. The base of the channel was found at a depth of 14–19 m under the present ground surface, descending from east to west draining the ice sheet front in the same direction. The channel is incised in the bedrock representing thus a Nye channel and is filled by >13 m of glaciofluvial sand, gravel and mud, which is covered by 1–2 m thick colluvial deposits. The large part of the glaciofluvial package was deposited in subglacial environment reflecting the ice sheet decay events of higher release of the material from the ice sheet. The upper glaciofluvial part of sediments was deposited in front of the slowly decaying ice sheet front. No subglacial tills have been found in the col area, which is not unusual for such an exposed site. We reconstruct the maximum altitude limit of the ice sheet in the col as being at least at ~540 m and the ice thickness in the Anděl Col being few tens of metres. The Anděl Col is the highest known area in Northern Bohemia covered by an ice sheet and improves thus our knowledge about the maximum vertical extent of Middle Pleistocene ice sheets in Western Sudetes.

Coupling the flow of ice, water, and sediment in a glacial landscape evolution model

The processes of subglacial erosion depend not only on the sliding motion of glaciers over bedrock but also on the presence of meltwater and sediment in the subglacial environment. In particular, theoretical models for subglacial quarrying and abrasion, as well as hypothesized erosion thresholds associated with subglacial sediment transport, include both positive and negative effects of subglacial water and sediment on the rate of erosion.In order to incorporate the existing theoretical models for subglacial erosion by quarrying and abrasion in a long-term glacial landscape evolution model, we here present a coupled computational framework for simulating the simultaneous flow processes of ice, water, and sediment. We supplement a higher order ice sheet model with simple long-term models for glacial hydrology and subglacial sediment transport, as well as fluvial and hillslope-related erosion processes.An important strength of the modeling framework presented relates to the morphological detail of the landscapes produced, which facilitates direct comparison with existing landforms. This improves the connection between glacial landscape evolution models, geomorphological observations, and the existing knowledge of the physical processes that operate under glaciers. We demonstrate with model examples, how increased basal meltwater pressure and transport-limited subglacial erosion lead to both positive and negative feedbacks related to glacial erosion and the formation of overdeepenings.

Modeling ice stream flow

Ice flow speed within an ice sheet can vary from a few meters to thousands of meters per year. Fast flowing ice streams can affect sea level, and their flow variation is one factor that determines whether an ice sheet is gaining or losing mass. But different ice streams exhibit different behaviors, and these spatial and temporal variations are not well understood, causing uncertainty about sea level change when ice streams stop or accelerate. Bougamont et al. used a three‐dimensional ice sheet model coupled with a model of the underlying subglacial environment to investigate the relationship between ice flow and basal till properties. This new coupled model reveals new and significant complexities in ice stream behavior, which are related to the spatial and temporal evolution of bed properties. Furthermore, they found that even small differences in the subglacial hydrology can significantly influence ice stream flow. Their results also reveal that ice streams may well have a memory insofar as their current flow is conditioned by their previous behavioral history and physical condition. The study is a step toward understanding the complex behavior of Antarctic ice streams. (Journal of Geophysical Research‐Earth Surface, doi:10.1029/2011JF002025, 2011)

POLYTHERMAL GLACIER HYDROLOGY: A REVIEW

The manner by which meltwater drains through a glacier is critical to ice dynamics, runoff characteristics, and water quality. However, much of the contemporary knowledge relating to glacier hydrology has been based upon, and conditioned by, understanding gleaned from temperate valley glaciers. Globally, a significant proportion of glaciers and ice sheets exhibit nontemperate thermal regimes. The recent, growing concern over the future response of polar glaciers and ice sheets to forecasts of a warming climate and lengthening summer melt season necessitates recognition of the hydrological processes in these nontemperate ice masses. It is therefore timely to present an accessible review of the scientific progress in glacial hydrology where nontemperate conditions are dominant. This review provides an appraisal of the glaciological literature from nontemperate glaciers, examining supraglacial, englacial, and subglacial environments in sequence and their role in hydrological processes within glacierized catchments. In particular, the variability and complexity in glacier thermal regimes are discussed, illustrating how a unified model of drainage architecture is likely to remain elusive due to structural controls on the presence of water. Cold ice near glacier surfaces may reduce meltwater flux into the glacier interior, but observations suggest that the transient thermal layer of near surface ice holds a hydrological role as a depth‐limited aquifer. Englacial flowpaths may arise from the deep incision of supraglacial streams or the propagation of hydrofractures, forms which are readily able to handle varied meltwater discharge or act as locations for water storage, and result in spatially discrete delivery of water to the subglacial environment. The influence of such drainage routes on seasonal meltwater release is explored, with reference to summer season upwellings and winter icing formation. Moreover, clear analogies emerge between nontemperate valley glacier and ice sheet hydrology, the discussion of which indicates how persistent reassessment of our conceptualization of glacier drainage systems is required. There is a clear emphasis that continued, integrated endeavors focused on process glaciology at nontemperate glaciers are a scientific imperative to augmenting the existing body of research centered on ice mass hydrology.

Characterization of yeast and filamentous fungi isolated from cryoconite holes of Svalbard, Arctic

Cryoconite holes have biogeochemical, ecological and biotechnological importance. This communication presents results on culturable psychrophilic yeast and filamentous fungi from cryoconite holes at Midre Lovenbreen glacier. The identification of these microbes was achieved through conventional and DNA sequencing techniques. Effect of temperature, salt and media on growth of the cultures was studied. Measurements on the bioavailability of nutrients and trace metals were made through different methods including ICPMS (Inductively Coupled Plasma Mass Spectrometry). Colony forming unit (CFU) per gram of sediment sample was calculated to be about 7 × 103–1.4 × 104 and 4 × 103–1.2 × 104 of yeast and filamentous fungi, respectively. Based on morphology and sequence data, these were identified as Cryptococcus gilvescens, Mrakia sp., Rhodotorula sp., Phialophora alba and Articulospora tetracladia. Amongst these, Phialophora alba, Cryptococcus gilvescens and Mrakia sp. zhenx-1 are reported for the first time from Svalbard Arctic, while Rhodotorula sp. (95% gene similarity) is a new species, yet to be described. Rhodotorula sp. expressed high amylase, while Cryptococcus gilvescens showed high lipase activity. Mrakia sp. showed phosphate solubilization between 4 and 15°C, which is a first record. Chemical analysis revealed the presence of organic carbon, nitrogen and phosphorus in substantial amounts in the sediments. Filamentous fungi and yeast in the cryoconite holes drive the process of organic macromolecule degradation through cold-adapted enzyme secretion, thereby assisting in nutrient cycling in these subglacial environments. Further, these cold-adapted enzymes may provide an opportunity for the prospect of biotechnology in Arctic. This is the first report on mycological investigation into cryoconite holes from Midre Lovenbreen glacier.

The 3D microscopic ‘signature’ of strain within glacial sediments revealed using X-ray computed microtomography

X-ray computed microtomography (μCT), a non-destructive analytical technique, was used to create volumetric three-dimensional (3D) models representing the internal composition and structure of undisturbed pro- and subglacial soft sediment sample plugs for the purposes of identifying and analysing kinematic indicators. The technique is introduced and a methodology is presented addressing specific issues relating to the investigation of unlithified, polymineralic sediments. Six samples were selected based on their proximity to ‘type’ brittle and ductile deformation structures, or because of their perceived suitability for successful application of the technique. Analysis of a proglacial ‘ideal’ specimen permitted the 3D geometry of a suite of micro-faults and folds to be investigated and the strain history of the sample reconstructed. The poor contrast achieved in scanning a diamicton of glaciomarine origin is attributable to overconsolidation under normal loading, the sediment demonstrated to have undergone subsequent subglacial deformation. Another overconsolidated diamicton contains an extensive, small scale (<20 μm) network of fractures delineating a ‘marble-bed’ structure, hitherto unknown at this scale. A volcanic lithic clast contrasts well with the surrounding matrix in a ‘lodgement’ till sample containing μCT (void) and thin-section evidence of clast ploughing. Initial ductile deformation was followed by dewatering of the matrix, which led to brittle failure and subsequent emplacement. Compelling evidence of clast rotation is located in the top of another sample, μCT analysis revealing that the grain has a proximal décollement surface orientated parallel to the plane of shear. The lenticular morphology of the rotational structure defined suggests an unequal distribution of forces along two of the principal stress axes. The excellent contrast between erratics contained within a sample and the enclosing till highlight the considerable potential of the technique in permitting the rapid (semi-)quantitative analysis of large datasets. The subglacial sample evidence indicates that complex, polyphase (brittle/ductile) deformation histories are common in such diamictic soft sediments and that the local (micro-scale) environment (composition, structure, shear forces and effective pressure) controls rheology. The sediment void ratio is a key indicator of strain. Three of the samples are tentatively placed at different points on the strain cycle for subglacially deforming soft sediment, based on their void ratio, characteristics and distribution. It has been demonstrated that μCT offers significant potential for elucidating glacial soft sediment kinematics. The ability of the technique to both test a variety of hypotheses pertaining to mechanisms operating within the subglacial environment and evaluate efforts to replicate those processes under controlled, laboratory conditions is therefore discussed along with solutions to the problems encountered within this project. μCT also permits the seamless linking of analytical techniques applied at the hand specimen (mm), micromorphological (μm-mm), scanning electron microscopy and X-ray diffraction/fluorescence (nm-μm) scales and the archiving, duplication and dissemination of sample volumetric 3D data.

The Microbial Habitability of Weathered Volcanic Glass Inferred from Continuous Sensing Techniques

Basaltic glasses (hyaloclastite) are a widespread habitat for life in volcanic environments, yet their interior physical conditions are poorly characterized. We investigated the characteristics of exposed weathered basaltic glass from a surface outcrop in Iceland, using microprobes capable of continuous sensing, to determine whether the physical conditions in the rock interior are hospitable to microbial life. The material provided thermal protection from freeze-thaw and rapid temperature fluctuations, similar to data reported for other rock types. Water activity experiments showed that at moisture contents less than 13% wet weight, the glass and its weathering product, palagonite, had a water activity below levels suitable for bacterial growth. In pore spaces, however, these higher moisture conditions might be maintained for many days after a precipitation event. Gas exchange between the rock interior and exterior was rapid (< 10 min) when the rocks were dry, but when saturated with water, equilibration took many hours. During this period, we demonstrated the potential for low oxygen conditions within the rock caused by respiratory stimulation of the heterotrophic community within. These conditions might exist within subglacial environments during the formation of the rocks or in micro-environments in the interior of exposed rocks. The experiments showed that microbial communities at the site studied here could potentially be active for 39% of the year, if the depth of the community within the outcrop maintains a balance between access to liquid water and adequate protection from freezing. In the absence of precipitation, the interior of weathered basaltic glass is an extreme and life-limiting environment for microorganisms on Earth and other planets.

Diversity, Abundance, and Potential Activity of Nitrifying and Nitrate-Reducing Microbial Assemblages in a Subglacial Ecosystem

Subglacial sediments sampled from beneath Robertson Glacier (RG), Alberta, Canada, were shown to harbor diverse assemblages of potential nitrifiers, nitrate reducers, and diazotrophs, as assessed by amoA, narG, and nifH gene biomarker diversity. Although archaeal amoA genes were detected, they were less abundant and less diverse than bacterial amoA, suggesting that bacteria are the predominant nitrifiers in RG sediments. Maximum nitrification and nitrate reduction rates in microcosms incubated at 4°C were 280 and 18.5 nmol of N per g of dry weight sediment per day, respectively, indicating the potential for these processes to occur in situ. Geochemical analyses of subglacial sediment pore waters and bulk subglacial meltwaters revealed low concentrations of inorganic and organic nitrogen compounds. These data, when coupled with a C/N atomic ratio of dissolved organic matter in subglacial pore waters of ~210, indicate that the sediment communities are N limited. This may reflect the combined biological activities of organic N mineralization, nitrification, and nitrate reduction. Despite evidence of N limitation and the detection of nifH, we were unable to detect biological nitrogen fixation activity in subglacial sediments. Collectively, the results presented here suggest a role for nitrification and nitrate reduction in sustaining microbial life in subglacial environments. Considering that ice currently covers 11% of the terrestrial landmass and has covered significantly greater portions of Earth at times in the past, the demonstration of nitrification and nitrate reduction in subglacial environments furthers our understanding of the potential for these environments to contribute to global biogeochemical cycles on glacial-interglacial timescales.

Antarctic grounding line mapping from differential satellite radar interferometry

[1] The delineation of an ice sheet grounding line, i.e., the transition boundary where ice detaches from the bed and becomes afloat in the ocean, is critical to ice sheet mass budget calculations, numerical modeling of ice sheet dynamics, ice-ocean interactions, oceanic tides, and subglacial environments. Here, we present 15 years of comprehensive, high-resolution mapping of grounding lines in Antarctica using differential satellite synthetic-aperture radar interferometry (DInSAR) data from the Earth Remote Sensing Satellites 1–2 (ERS-1/2), RADARSAT-1 and 2, and the Advanced Land Observing System (ALOS) PALSAR for years 1994 to 2009. DInSAR directly measures the vertical motion of floating ice shelves in response to tidal oceanic forcing with millimeter precision, at a sample spacing better than 50 m, simultaneously over areas several 100 km wide; in contrast with earlier methods that detect abrupt changes in surface slope in satellite visible imagery or altimetry data. On stagnant and slow-moving areas, we find that breaks in surface slope are reliable indicators of grounding lines; but on most fast-moving glaciers and ice streams, our DInSAR results reveal that prior mappings have positioning errors ranging from a few km to over 100 km. A better agreement is found with ICESat's data, also based on measurements of vertical motion, but with a detection noise one order of magnitude larger than with DInSAR. Overall, the DInSAR mapping of Antarctic grounding lines completely redefines the coastline of Antarctica.

Early and middle Miocene Antarctic glacial history from the sedimentary facies distribution in the AND-2A drill hole, Ross Sea, Antarctica

In 2007, the Antarctic Geological Drilling Program (ANDRILL) drilled 1138.54 m of strata ~10 km off the East Antarctic coast, includ ing an expanded early to middle Miocene succession not previously recovered from the Antarctic continental shelf. Here, we pre sent a facies model, distribution, and paleoclimatic interpretation for the AND-2A drill hole, which enable us, for the fi rst time, to reconstruct periods of early and middle Miocene glacial advance and retreat and paleo environmental changes at an ice-proximal site. Three types of facies associations can be recognized that imply signifi cantly different paleoclimatic interpretations. (1) A diamictite-dominated facies association represents glacially dominated depositional environments, including subglacial environments, with only brief intervals where ice-free coasts existed, and periods when the ice sheet was periodically larger than the modern ice sheet. (2) A stratifi ed diamictite and mudstone facies association includes facies characteristic of open-marine to iceberg-infl uenced depositional environments and is more consistent with a very dynamic ice sheet, with a grounding line south of the modern position. (3) A mudstone-dominated facies association generally lacks diamictites and was produced in a glacially infl uenced hemipelagic depositional environment. Based on the distribution of these facies associations, we can conclude that the Antarctic ice sheets were dynamic, with grounding lines south of the modern location at ca. 20.1‐19.6 Ma and ca. 19.3‐18.7 Ma and during the Miocene climatic optimum, ca. 17.6‐15.4 Ma, with ice-sheet and sea-ice minima at ca. 16.5‐16.3 Ma and ca. 15.7‐15.6 Ma. While glacial minima at ca. 20.1‐19.6 Ma and ca. 19.3‐18.7 Ma were characterized by temperate margins, an increased abundance of gravelly facies and diatomaceous siltstone and a lack of meltwater plume deposits suggest a cooler and drier climate with polythermal conditions for the Miocene climatic optimum (ca. 17.6‐15.4 Ma). Several periods of major ice growth with a grounding line traversing the drill site are recognized between ca. 20.2 and 17.6 Ma, and after ca. 15.4 Ma, with evidence of cold polar glaciers with ice shelves. The AND-2A core provides proximal evidence that during the middle Miocene climate transition, an ice sheet larger than the modern ice sheet was already present by ca. 14.7 Ma, ~1 m.y. earlier than generally inferred from deep-sea oxygen isotope records. These fi ndings highlight the importance of high-latitude ice-proximal records for the interpretation of far-fi eld proxies across major climate transitions.

Relative Incidence of Ascomycetous Yeasts in Arctic Coastal Environments

Previous studies of fungi in polar environments have revealed a prevalence of basidiomycetous yeasts in soil and in subglacial environments of polythermal glaciers. Ascomycetous yeasts have rarely been reported from extremely cold natural environments, even though they are known contaminants of frozen foods. Using media with low water activity, we have isolated various yeast species from the subglacial ice of four glaciers from the coastal Arctic environment of Kongsfjorden, Spitzbergen, including Debaryomyces hansenii and Pichia guillermondii, with counts reaching 10(4)CFUL(-1). Together with the basidiomycetes Cryptococcus liquefaciens and Rhodotorula mucilaginosa, these yeasts represent the stable core of the subglacial yeast communities. Other glacial ascomycetous species isolated included Candida parapsilosis and a putative new species that resembles Candida pseudorugosa. The archiascomycete Protomyces inouyei has seldom been detected anywhere in the world but was here recovered from ice in a glacier cave. The glacier meltwater contained only D. hansenii, whereas the seawater contained D. hansenii, Debaryomyces maramus, Pichia guilliermondii, what appears to represent a novel species resembling Candida galli and Metschnikowia bicuspidata. Only P. guilliermondii was isolated from sea ice, while snow/ice in the fjord tidal zone included C. parapsilosis, D. hansenii, P. guilliermondii and Metschnikowia zobellii. All of these isolated strains were characterized as psychrotolerant and xero/halotolerant, with the exception of P. inouyei.