Subcritical Butane Extraction Research Articles

Effect of oil extraction methods on walnut oil quality characteristics and the functional properties of walnut protein isolate

Walnut oils were obtained by supercritical carbon dioxide extraction (SCB), cold-pressing (CP), hexane extraction (HE), and subcritical butane extraction (SBE), and walnut protein isolates (WPI) from the walnut cakes were performed. The results indicate that SCB has the highest oil yield for walnut oil, which was 62.72%, and the total content of trace nutrients (total tocopherols, total phytosterols, and total phenolic compounds) in SCB-walnut oil was also the highest at 2186.75 mg/kg, approximately 1.05 times higher than CP-walnut oil and 1.21 times higher than SBE-walnut oil. Meanwhile, the treatment of WPI with SCB results in a decrease in β-Sheet and α-Helix structures and an increase in β-Turn and Random coil structures. Thereby increasing its oil-holding capacity (OHC) and solubility by approximately 1.16 times and 1.27 times compared to CP, respectively. Interestingly, SCB as a green oil production technology, also has good prospects for retaining WPI functionality characteristics.

High-oleic rapeseed oil quality indicators and endogenous antioxidant substances under different processing methods.

This study exposed high-oleic rapeseed oil (HORO) to different pretreatment (microwave or roasting) and processing methods to investigate (cold pressing, hexane extraction, subcritical butane extraction, and aqueous enzymatic extraction) the effects of processing technologies on HORO parameters associated with its physicochemical properties, endogenous antioxidant substances, and antioxidant capacity. The oil yield of various processing technologies was between 35.4% and 59.7%, and the fatty acid composition did not significantly differ. Hierarchical clustering and principal component analyses were used for evaluation. The results revealed that the microwave pretreatment-hexane extraction (M-HE) method resulted in significantly higher levels of tocopherols (688.4mg/kg), polyphenols (1007.76mg/kg), and phytosterols (1810.6mg/kg) in HORO, implying strong free radical scavenging capacity (DPPH-oil: 79.63, DPPH-nonpolar: 71.42, DPPH-polar: 6.65, FRAP: 55.4, ABTS: 3043.7μmol TE/kg). Hence, M-HE is a promising method for producing HORO with a higher stability and nutritional value.

Subcritical butane extraction of acer truncatum seed oil: Kinetics, thermodynamics, and optimization

In this study, the kinetics, thermodynamics, and optimization of the subcritical butane extraction of Acer truncatum seed oil were investigated. The effects of experimental parameters on the extraction yield and efficiency was studied and optimized by response surface methodology, with the optimum time, temperature, and liquid/solid ratio (mL/g) of 44 min, 52 °C, and 3.4, respectively. The extraction efficiency could reach up to 94.50 % after twice extraction under optimized conditions. The extraction kinetics was determined by a modified Fick’s law model and the activation energy of the effective diffusion coefficient was obtained as 23.26 kJ/mol. The enthalpy change and the entropy change were both positive with values of 20.06 kJ/mol and 70.02 J/(mol·K), respectively, while The Gibbs free energy change was negative in the temperature range. Finally, the comparisons of extraction efficiency, fatty acid composition, and properties were made for subcritical extraction and other three methods.

Optimization of extraction factor and nutritional characterization of black soldier fly larvae oil via subcritical butane extraction

This study aimed to optimize the subcritical butane extraction of black soldier fly larvae (Hermetia illucens) using response surface methodology (RSM) and evaluate the nutritional properties of oil (BSFLO) and meal (BSFLM) products. A Box-Behnken design was conducted to reveal the effects of temperature (25–65 °C), extraction time (20–60 min) and number of extractions (1–5 times) on oil yield. Results showed that oil yield was significantly affected by number of extractions as well as the interaction of extraction time and number of extractions. The optimal conditions were 35 °C, 50 min/time and 5 times with the extraction yield of 31.19% (dry basis). Nutritional properties indicated that subcritical butane extraction did not change the fatty acids composition of BSFLO and amino acids composition of BSFLM. The total amino acid of BSFLM produced by subcritical butane extraction was enhanced due to the more lipid was separated. However, the nitrogen solubility index and protein dispersibility index of BSFLM decreased, which may negatively affect the protein utilization. To conclude, subcritical butane extraction provided an available and promising method for further processing of black soldier fly larvae.

Influence of different extraction methods on the chemical composition, antioxidant activity, and overall quality attributes of oils from Trichosanthes kirilowii Maxim seed

Trichosanthes kirilowii Maxim seed oil is full of nutrition and exerts positive effects on the health of humans. The study detected and compared the quality characteristics, the lipid composition and active constituents of Trichosanthes kirilowii Maxim seed kernel and shell oil. This study used four extraction techniques: cold pressing, ultrasonic-assisted solvent extraction, supercritical fluid extraction, and subcritical butane extraction. The results indicated that the highest lipid yield (36.75%) was obtained by ultrasonic-assisted solvent extraction, while the lowest (32.24%) was obtained by supercritical fluid extraction. Meanwhile, Trichosanthes kirilowii Maxim seed oil is rich in linoleic acid (25.64–31.24%) and α-tocopherol (247.76–702.28 mg/kg). The extraction method and shells significantly affected beneficial phytochemicals (tocopherols, phytosterols and total phenols) and antioxidant activity (DPPH) (p < 0.05). Moreover, the tocopherols of supercritical fluid extraction oil (1020.15–1052.55 mg/kg) and the phytosterols of cold pressing oil (1033.84–1748.20 mg/kg) were higher than that of other methods under the same conditions. The content of sterols and tocopherol in the seed kernel oil is higher than that of the seed shell oil. Meanwhile, cold pressing oil had more abundant volatile compounds. The above information is valuable for manufacturers to select the appropriate method to produce Trichosanthes kirilowii Maxim seed oil with the desired quality attributes and chemical compositions for the target end-user.

Assessment of oil extracted from Gardenia fruits by different commercial extraction methods for potential industrial applications

Gardenia fruit is an extraordinary functional oil-bearing material product with few research on its potential for the direct production of industrial oil-bearing products. This study compared the physicochemical properties, lipid properties and thermal/oxidative stability of gardenia fruit oil produced by subcritical butane extraction (SBE) and cold-pressed extraction (CPE). The results indicated that the gardenia oil extracted by the above two methods had excellent physicochemical properties; where C18:2, C18:1 and C18:3 were dominant fatty acids in the fruit oil. The infrared spectrum showed high-intensity bands at 3009 cm−1-2855 cm−1 and 1746 cm−1, representing chemical groups of total oil and fat and unsaturated fatty acid (UFAs). Indexes such as atherogenic index (AI), thrombotic index (TI) and hypocholesterolemic/hypercholesterolemic fatty acids (HH) revealed that gardenia oil might benefit human health by lowering total cholesterol. The oil contained 7 kinds of phytosterols (dominated by beta-sitosterol) and a high level of squalene, vitamin E, carotenoids, polyphenols and total flavonoids. It also had advantageous initial/maximum degradation temperatures and stable oxidative induction time, which suggested its potential as a raw material for edible products, chemical and pharmaceutical industries. The oil produced by SBE was superior to the former (P < 0.05) in terms of oil extraction rate, concomitant product content and thermal/oxidative stability. This indicated that the SBE technology was an ideal alternative for the industry to produce gardenia oil with very good physiological activity.

Characteristics and Antioxidant Activity of Walnut Oil Using Various Pretreatment and Processing Technologies.

This study was the first time the effects of pretreatment technology (microwave roasting, MR; oven roasting, OR; steaming roasting, SR) and processing technology (screw pressing, SP; aqueous enzymatic extraction, AEE; subcritical butane extraction, SBE) on the quality (physicochemical properties, phytochemical content, and antioxidant ability) of walnut oil were systematically compared. The results showed that the roasting pretreatment would reduce the lipid yield of walnut oil and SBE (59.53–61.19%) was the processing method with the highest yield. SR-AEE oil provided higher acid value (2.49 mg/g) and peroxide value (4.16 mmol/kg), while MR-SP oil had the highest content of polyunsaturated fatty acid (73.69%), total tocopherol (419.85 mg/kg) and total phenolic compounds (TPC, 13.12 mg/kg). The DPPH-polar and ABTS free radicals’ scavenging abilities were accorded with SBE > AEE > SP. SBE is the recommended process for improving the extraction yield and antioxidant ability of walnut oil. Hierarchical cluster analysis showed that processing technology had a greater impact on walnut oil than pretreatment technology. In addition, multiple linear regression revealed C18:0, δ-tocopherol and TPC had positive effects on the antioxidant ability of walnut oil, while C18:1n-9, C18:3n-3 and γ-tocopherol were negatively correlated with antioxidant activity. Thus, this a promising implication for walnut oil production.

Optimization of the Subcritical Butane Extraction of Tung Oil and Its Mechanism Analysis

Tung oil is an important dry grease. In order to overcome the deficiencies of traditional processes in oil production, the preparation of tung oil was carried out by the butane-subcritical method. A response surface optimization experiment was carried out based on Design-Expert software, and the best process parameters were obtained. The extraction temperature was 42.98 °C, the extraction time was 43.77 min, the particle size of the raw material was 38.88 mesh, and the oil yield of tung oil under this condition reached 67.437%. The fatty acid composition of tung oil was analyzed by Gas Chromatography-Mass Spectrometry (GC-MS): the content of α-oleostearic acid was 74.99%, linoleic acid content was 8.83%, oleic acid content was 7.42%, palmitic acid content was 2.02%, and stearic acid content was 4.35%. Through the analysis of the oil sample obtained, five indicators showed that the process of obtaining oil products met the requirements of the national standard. By simulating the subcritical n-butane/tung oil dissolution equilibrium model, the miscible dynamic equilibrium of tung oil in subcritical n-butane was studied at temperatures in the range of 35–50 °C and an equilibrium time of 40 min, and the kinetic equations of oil extraction at different temperatures were obtained, with a coefficient of determination (R2) greater than 0.99. The oil extraction rate was up to 67.12 ± 0.05% under optimal extraction conditions through the optimization of univariate and response surface experimental design. Using 1stOpt data processing software, the data of tung oil extraction rate at different times were fitted, and it was found that the Patricelli model accurately elucidated the kinetic process of tung oil extraction through subcritical n-butane, with R2 greater than 0.99.

Key chemical composition of walnut (Juglans regia. L) Oils generated with different processing methods and their cholesterol-lowering effects in HepG2 cells

We systematically evaluated fatty acids and triacylglycerol composition, as well as tocopherol, phytosterol, and phenolics, in walnut oil and compared the cholesterol-lowering effects of oil processed with different methods (cold pressing, roast-pressing, hexane extraction, subcritical butane extraction, and supercritical CO2 extraction). The different methods did not affect the lipid composition of walnut oil. The tocopherol (41.11 mg/100 g) and total phenolic content (TPC, 4.26 mg/100 g) of roast-pressed walnut oil and the phytosterol contents of subcritical butane-extracted walnut oil (106.51 mg/100 g) were higher than those of other tested oils. Walnut oil significantly decreased cholesterol synthesis by downregulating the expression of HMGCR, SREBP-2, and CYP51 genes, and increased cholesterol efflux by upregulating the expression of ABCG1, thus significantly reducing total cholesterol and triacylglycerol. Phytosterols and TPC in walnut oil were responsible for lowering cholesterol; the optimal concentration of phytosterols was 10 μg/mL, and that of TPC was 12.5 × 10−3 μg/mL. Through process optimization, a new processing method for walnut oil based on biological evaluation was preliminarily established.

Effects of processing methods on the chemical composition and antioxidant capacity of walnut (Juglans regia L.) oil

Various methods for the processing of walnut oil (cold pressing, roasting pressing, hexane extraction, subcritical butane extraction and supercritical carbon dioxide extraction) were compared, and hexane extraction was shown to provide for efficient lipid isolation. Among the investigated samples, roast-pressed and hexane-extracted oils exhibited the highest C18:1n-9 contents (18.74 and 18.52%, respectively), however featured the lowest C18:2n-6 (63.06 and 62.95%, respectively) and trilinolein (32.82 and 32.06%, respectively) contents. Moreover, the tocopherol (371.08 mg/kg) and the phytosterol (1206.30 mg/kg) content of subcritical butane-extracted walnut oil, the polyphenol content of hexane-extracted oil (45.43 mg/kg), and the squalene content of roast-pressed walnut oil (14.19 mg/kg) exceeded those of other samples. Multiple linear regression analysis was employed to evaluate the contributions of oil constituents to overall antioxidant capacity (oxidative stability and free radical scavenging capacity) and thus establish the corresponding numerical model. It was shown that C16:0, polyphenol, Δ5-avenasterol, SOS, γ-tocopherol, PLL, C18:3n-3, LLL, and C18:0 contributed to the antioxidant capacity of the walnut oil. Overall, the obtained results pave the way to the development of industrial-scale methods of high-quality and high-nutritional-value walnut oil production.

A novel and efficient subcritical butane extraction method and UHPLC analysis of oxyprenylated phenylpropanoids from grapefruits peels

Biologically active prenyoxyphenylpropanoids are well known to be biosynthesized by Citrus species, for which they have been found most abundantly in fruit peels. Although several extraction methodologies have been described, the development of novel and alternative extraction processes is a field of research of current interest. In this preliminary communication, we studied the performance of the subcritical butane promoted extraction of selected oxyprenylated phenylpropanoids from grapefruit peels under a counter-current mode using a handmade extraction apparatus coupled to UHPLC analysis. The application of such a method yielded 7-isopentenyloxycoumarin, auraptene, and boropinic acid in quantities higher than those recorded for other extraction methodologies like the ultrasound- and microwave-assisted macerations (0.234, 1.035, and 0.211 mg/g of dry extract respectively). The use of subcritical butane as the extraction solvent for oxyprenylated phenylpropanoids is reported herein for the first time and can be easily adopted for several other food matrices.

The Essential Oils and Eucalyptol From Artemisia vulgaris L. Prevent Acetaminophen-Induced Liver Injury by Activating Nrf2-Keap1 and Enhancing APAP Clearance Through Non-Toxic Metabolic Pathway.

Artemisia has long been used in traditional medicine and as a food source for different functions in eastern Asia. Artemisia vulgaris L. (AV) is a species of the genus Artemisia. Essential oils (EOs) were extracted from AV by subcritical butane extraction. EO contents were detected by electronic nose and headspace solid-phase microextraction coupled with gas chromatography (HS-SPME-GC-MS). To investigate the hepatoprotective effects, mice subjected to liver injury were treated intragastrically with EOs or eucalyptol for 3 days. Acetaminophen (APAP) alone caused severe liver injury characterized by significantly increased serum AST and ALT levels, ROS and hepatic malondialdehyde (MDA), as well as liver superoxide dismutase (SOD) and catalase (CAT) depletions. EOs significantly attenuated APAP-induced liver damages. Further study confirmed that eucalyptol is an inhibitor of Keap1, the affinity K D of eucalyptol and Keap1 was 1.42 × 10−5, which increased the Nrf2 translocation from the cytoplasm into the mitochondria. The activated Nrf2 increased the mRNA expression of uridine diphosphate glucuronosyltransferases (UGTs) and sulfotransferases (SULTs), also inhibiting CYP2E1 activities. Thus, the activated Nrf2 suppressed toxic intermediate formation, promoting APAP hepatic non-toxicity, whereby APAP was metabolized into APAP-gluc and APAP-sulf. Collectively, APAP non-toxic metabolism was accelerated by eucalyptol in protecting the liver against APAP-induced injury, indicating eucalyptol or EOs from AV potentials as a natural source of hepatoprotective agent.

The comparison of krill oil extracted through ethanol-hexane method and subcritical method.

This study aimed to develop a safe method EH (ethanol–hexane) to extract two kinds of krill oil (KO) simultaneously and analyze their composition. Meanwhile, subcritical butane and subcritical butane‐dimethyl ether extraction were used to extract KO for analysis comparison. Folch method was used to extract total lipids. When the volume ratio of ethanol to hexane is 4:6, the separation effect of ethanol layer and hexane layer is best. At this condition, the EH method yielded similar amount of lipids (up to 97. 72% of total lipids) with subcritical butane extraction method (97.60%). The recovery rate of ethanol and hexane was 83.6% and 86.86%, respectively. KO in hexane layer and extracted by the subcritical butane method are abundant in astaxanthin (910 and 940 mg/kg respectively), while KO in the ethanol layer had the highest phospholipid (PL) content (47.34%), n−3 polyunsaturated fatty acids (PUFA) content (45.51%), and the lowest fluorine content (11.17 μg/g), making it a potential candidate in the nutraceutical and antioxidant industry.

Comparison of Different Processing Methods of Iron Walnut Oils (Juglans sigillata): Lipid Yield, Lipid Compositions, Minor Components, and Antioxidant Capacity

A comparison study is performed on different processing methods of iron walnut oils (Juglans sigillata). Processing methods here include cold‐pressing (CP), roasting‐pressing (RP), n‐hexane extraction (HE), subcritical butane extraction (SBE), and supercritical carbon dioxide extraction. The lipid yield, physicochemical properties, lipid compositions, minor components, and antioxidant capacity of iron walnut oils are analyzed. Different processing methods do not affect the fatty acid compositions and triacylglycerol compositions of iron walnut oil. The lipid yields of pressing process (42.63–44.12%) is lower than that of the extraction process (48.13–63.44%). By multiple linear regression analysis, the results about the correlation between minor components and antioxidant capacity show that the RP process with high number of polyphenols (52.91 mg kg−1) indicates the highest antioxidant activity (DPPH: 574.73, FRAP: 111.80, ABTS: 498.53, ORAC: 523.34 µmol TE kg−1, respectively). Considering health and nutritional value of oil, RP can be a promising method for future iron walnut oil processing.Practical Applications: Iron walnut (Juglans sigillata) is a special walnut variety in China. In this study, five different processing methods of iron walnut oil are evaluated. The new iron walnut oil processing method is beneficial to the development of iron walnut oil. The authors show that lipid composition of tested oils is relatively stable, while their minor components contents are significantly different. Oil antioxidant capacity is mainly related to polyphenols. Roasting‐pressing can obviously improve the antioxidant activity of the iron walnut oil. This information has important directive to the nutritional value and industrial production of iron walnut oil.Iron walnut oil is obtained from Juglans sigillata by different processing methods, including cold‐pressing, roasting‐pressing, n‐hexane extraction, subcritical butane extraction, and supercritical carbon dioxide extraction. The lipid yield, physicochemical properties, lipid compositions, minor components, and antioxidant capacity (DPPH, FRAP, ABTS, and ORAC) are analyzed. Models deduced by multiple linear regression are recognized as acceptable for predicting the antioxidant capacity of the iron walnut oil.

Process Optimization, Characterization and Antioxidant Capacity of Oat (Avena Sativa L.) Bran Oil Extracted by Subcritical Butane Extraction.

Oat bran is a traditional agricultural byproduct and rarely used in edible oil processing. In this paper, oat bran oil (OBO) was firstly extracted by subcritical butane extraction (SBE) and the extraction process was optimized using response surface methodology. Three variables involving liquid-to-solid ratio, extraction time and extraction temperature were studied. The optimum conditions for extraction of OBO were obtained as follows: liquid-to-solid ratio 4.30, extraction time 48.15 min, and extraction temperature 46.52 °C. Based on this, an alternative method (SBE-e) for cosolvent (ethanol) was proposed to improve SBE method. Compared to conventional hexane extraction (CHE), the SBE-e had significant effect on yield, bioactive compounds (phytosterols and phenols) and antioxidant capacity (AC) in the extracted OBO. The results indicated that the proposed methods were appropriate for OBO extraction. Additionally, OBO had the potential to be an acceptable substitute for edible oil, owing to its desirable physicochemical characteristics, a balanced fatty acids composition and high antioxidant capacity.

Oxidative Stability of Papaya Seed Oil From Hainan/Eksotika Obtained by Subcritical and Supercritical Carbon Dioxide Extraction

AbstractPapaya seed is a good source of edible oil with considerable antioxidant activity. Here, papaya seed oil (PSO) of Hainan/Eksotika variety was obtained by subcritical butane extraction (SBE) and supercritical carbon dioxide extraction (SCDE), and its yields, physicochemical properties, oxidative and thermal stability, and chemical and microscopic structures were compared. The results showed that SBE‐PSO had a higher yield (25.88 ± 0.29% vs. 19.47 ± 0.92%), but a lower melting point compared to SCDE‐PSO. Molecular structures indicated that there was no oxidative degradation during SBE and SCDE. Both SBE and SCDE caused significant structural changes of seed tissues. In addition, aldehyde composition analysis using high performance liquid chromatography (HPLC) showed that SBE‐PSO had fewer octanal, nonanal, 2‐decenal, and 2‐undecenal contents than SCDE‐PSO. All these results proved that SBE‐PSO exhibited superiority on the oxidative stability compared to SCDE‐PSO. It indicated that SBE was a superior method to obtain antioxidant edible oil with good stability.

Subcritical butane extraction of oil and minor bioactive components from soybean germ: Determination of migration patterns and a kinetic model

AbstractFor the aim of determining the migration patterns and extraction kinetics of the oil and minor bioactive components during subcritical fluid extraction (SFE) process from soybean germ, the extraction efficiency was investigated at different temperature and time with butane as solvent. The results indicate that flaking treatment is beneficial to the release of soybean germ oil (SGO) and bioactive components. The chosen models (So and Macdonald's model for SGO, Baümler's model for bioactive components) were found to be in good agreement with the experimental data and could be used to explain the SGO and bioactive components migration during SFE process. Furthermore, the activation energy of phytosterols, tocopherols, and phospholipids were 13.42, 12.23, and 22.72 kJ/mol, respectively. In conclusion, high‐quality SGO that contain high levels of phytosterols (26.51 mg/g) and tocopherols (2.29 mg/g) and a lower content of phospholipids (26.68 mg/g) can be obtained with an extraction at 60 °C for 45 min.Practical applicationsIn this study the effects of pretreatment of SG, solid/liquid ratio, extraction temperature and time on the yields of SGO and minor bioactive components were investigated. Simultaneously, the migration patterns and kinetic models of SGO and minor bioactive components described the subcritical butane extraction process accurately. Therefore, the research provided a theoretical support for the pretreatment of raw materials and the parameters control of subcritical extraction of soybean germ oil. The study can guide the popularization and application of subcritical fluid extraction for soybean germ oil extraction effectively. At present, the technology is ready for technical transformation in Henan subcritical fluid extraction company.

Antioxidant, Anti-microbial Properties and Chemical Composition of Cumin Essential Oils Extracted by Three Methods

Abstract The purpose of this study is to evaluate the chemical composition, antioxidant and anti-bacterial activity of cumin essential oils (CEOs) extracted by different techniques, including supercritical carbon dioxide extraction (SCE), subcritical butane extraction (SBE) and traditional solvent extraction (SE). Our results indicated that CEOs are a valuable source of bioactive compounds, including cumin aldehyde, γ-terpinene and β-pinene. The most abundant components found in CEOs obtained by SCE and SBE were similar, while the abundant components in SE, β-Cumic aldehyde (19.31%) and α-phellandrene (9.49%), were distinctive. CEOs obtained by SCE exhibited higher antioxidant activity, followed by those extracted by SE and SBE. Moreover, the anti-microbial properties of CEOs obtained by SCE and SBE were higher than that of CEOs collected by SE. In conclusion, CEOs exhibit strong antioxidant and anti-microbial properties, which suggests a potential role of CEOs in preventing diseases associated with aging and oxidative stress, and our results highlight the potential usage of CEOs in the food industry.

Effective Subcritical Butane Extraction of Bifenthrin Residue in Black Tea.

As a natural and healthy beverage, tea is widely enjoyed; however, the pesticide residues in tea leaves affect the quality and food safety. To develop a highly selective and efficient method for the facile removal of pesticide residues, the subcritical butane extraction (SBE) technique was employed, and three variables involving temperature, time and extraction cycles were studied. The optimum SBE conditions were found to be as follows: extraction temperature 45 °C, extraction time 30 min, number of extraction cycles 1, and in such a condition that the extraction efficiency reached as high as 92%. Further, the catechins, theanine, caffeine and aroma components, which determine the quality of the tea, fluctuated after SBE treatment. Compared with the uncrushed leaves, pesticide residues can more easily be removed from crushed leaves, and the practical extraction efficiency was 97%. These results indicate that SBE is a useful method to efficiently remove the bifenthrin, and as appearance is not relevant in the production process, tea leaves should first be crushed and then extracted in order that residual pesticides are thoroughly removed.

Extraction of Fenugreek (Trigonella foenum-graceum L.) Seed Oil Using Subcritical Butane: Characterization and Process Optimization.

In this study, the subcritical butane extraction process of fenugreek seed oil was optimized using response surface methodology with a Box-Behnken design. The optimum conditions for extracted oil from fenugreek seed was as follows: extraction temperature of 43.24 °C, extraction time of 32.80 min, and particle size of 0.26 mm. No significant differences were found between the experimental and predicted values. The physical and chemical properties of the oil showed that the oil could be used as edible oil. Fatty acid composition of oils obtained by subcritical butane under the optimum conditions and by accelerated solvent extraction showed negligible difference. The oils were rich in linoleic acid (42.71%–42.80%), linolenic acid (26.03%–26.15%), and oleic acid (14.24%–14.40%). The results revealed that the proposed method was feasible, and this essay shows the way to exploit fenugreek seeds by subcritical butane extraction under the scope of edible oils.