Abstract. A comprehensive understanding of spatiotemporal runoff changes in the Yarlung Zangbo (YZ) basin in the southern Tibetan Plateau (TP) at a sub-basin scale, amidst varying climatic and cryospheric conditions, is imperative for effective water resources management. However, spatiotemporal differences of runoff composition and change and their attribution within the YZ basin have not been extensively explored, primarily due to the lack of hydrometeorological observations, especially in the downstream region. In this study, we investigated historical and future evolution of annual and seasonal total water availability, as well as glacier runoff and snowmelt contributions across six sub-basins of the YZ, with a particular focus on the comparison between the upstream Nuxia (NX) basin and the downstream Nuxia–Pasighat (NX-BXK) basin, based on a newly generated precipitation dataset and a well-validated model with streamflow, glacier mass, and snow cover observations. Our findings revealed that large spatiotemporal differences in changes exist within the YZ basin for 1971–2020. Firstly, runoff generation was dominated by rainfall runoff throughout the YZ basin, with glacier runoff playing a more important role in the annual total runoff (19 %) in the NX-BXK sub-basin compared to other sub-basins. Notably, glacier runoff contributed 52 % of the total runoff at the Pasighat outlet of the YZ basin. Secondly, annual runoff exhibited an increasing trend in the NX basin but a decreasing trend in the NX-BXK, primarily attributed to rainfall runoff changes influenced by atmospheric moisture. Glacier runoff enhanced water supply by offsetting the decreasing contribution from rainfall. Total runoff will consistently increase (27–100 mm (10 yr)−1) across the sub-basins through the 21st century, resulting from increased rainfall runoff and a minor effect of increased snowmelt and glacier runoff.