Sub-acute Toxicity Profile Research Articles

Safety evaluation of Avicenna germinans leaf extracts in rats

BackgroundAvicenna germinans is one of Nigeria's dominant mangrove plants and has valuable pharmacological and therapeutic activity. It is widely employed in traditional medicine to treat many ailments and diseases, including cancer. However, little is known about the safety of the plant. Acute and sub-acute toxicity profiles of the plant extracts were assessed in this study. MethodsThe oral acute toxicity was determined with 5000 mg/kg body weight each of n-hexane, ethyl acetate, and ethanol extracts of Avicenna germinans leaf in rats. The rats were monitored for mortality, clinical manifestations, and weight changes over 14 days. During the sub-acute toxicity study, twenty-five female rats were fed with 0–1000 mg/kg ethanol extracts of Avicenna germinans for 21 days. The body weight was monitored during treatment, while changes in gross organ morphology, relative organ weights, hematology, serum biochemistry, and limited histological analysis were evaluated after treatment. ResultsAcute administration of the three extracts did not result in mortality or any obvious adverse effects. The gross pathology, relative organ weights, hematology, serum biochemistry, and histopathology were similar to the control, except for the 1000 mg/kg dose that evoked marked increases in relative heart, intestinal, thymus, and adrenal weights. In addition, foci of inflammatory cells in the interstitium and lymphoid hyperplasia were found in the heart and lungs, respectively, in the 1000 mg/kg group. ConclusionThe findings indicate that LD50 of the three extracts of Avicenna germinans were > 5000 mg/kg and sub-acute administration of the ethanol extract is relatively safe at least up to 750 mg/kg body weights.

Harmony in nature's elixir: a comprehensive exploration of ethanol and nano-formulated extracts from Passiflora incarnata leaves: unveiling in vitro cytotoxicity, acute and sub-acute toxicity profiles in Swiss albino mice.

We analyzed the toxic effect of the ethanolic extract of Passiflora incarnata (EEP) and its nanoformulation (N-EEP) in the in vitro and in vivo models (zebrafish embryos and Swiss albino mice). The EEP composition was verified by phytochemical and GC-MS analysis. The synthesized N-EEP was characterized using UV-visible spectroscopy and scanning electron microscopy. In vitro results showed both EEP and N-EEP have a dose-dependent effect in L132 cells (normal embryonic lung cells). In zebrafish embryos, no developmental changes were observed for both EEP and N-EEP at 200µg/ml. The acute and sub-acute toxicity of EEP and N-EEP was identified by oral administration in Swiss albino mice. A single-day oral dose of EEP and N-EEP at different concentrations was administered for acute toxicity, and changes in body weight, food, water intake, temperature, respiration rate, skin color changes, and eye color till 72h was observed. In a sub-acute toxicity study, 28days oral administration of different concentrations of EEP and N-EEP was done. Hematological analysis, serum hepatic biochemical parameter analysis, and histopathological analysis for the liver, kidney, spleen, intestine, and heart were performed. The results indicated that lower than 600mg/kg of EEP and N-EEP can safely be used for the remediation of a spectrum of diseases.

Acute and sub-acute Toxicity Profiles of Anchusa strigosa and Zataria Multiflora: Insights from wistar albino rats

The present study aims to assess the acute and subacute toxicity of the hydro-alcoholic extracts of Anchusa strigosa (leaves) and the aerial parts of Zataria multiflora Boiss in Wistar albino rats. The crude extracts of Anchusa strigosa (leaves) and the aerial parts of Zataria multiflora Boiss were prepared in 70% ethanol. Systematic tests for acute toxicity were performed at varying dosages of 100, 250, and 500 mg/kg, while for subacute toxicity, a dose of 600 mg/kg was orally given to Wistar albino rats. At the end of acute and sub-acute toxicity studies, biochemical parameters, hematological analysis, and histopathological analysis showed no significant difference in the body weight, abnormalities, or organ damage of the rats compared to the untreated rats (control). Also, there were no results of death recorded in rats. These findings indicated that the medium-term oral administration of Anchusa strigosa (leaves) and the aerial parts of Zataria multiflora Boiss after the treatment does not cause toxicity and provides assurance regarding their suitability for potential therapeutic applications in both acute and subacute forms.

Genotoxicity, acute and sub-acute toxicity profiles of methanolic Cordyceps militaris (L.) Fr. extract in Swiss Albino Mice

Ethnopharmacological relevanceCordyceps militaris, a traditional medicinal fungus, parasitizes the intestines of lepidopteron pupae or larvae, predominantly during the winter, and undergoes fruiting in the summer or autumn. Compounds extracted from C. militaris have demonstrated a broad spectrum of pharmacological effects, including antioxidant, anti-tumor, anti-metastatic, anti-inflammatory, antiviral, anti-diabetic, and various others. Aim of the studyHerein, our study aimed at elucidating the acute, sub-acute toxicity, and genotoxicity profiles of C. militaris methanolic extract following oral administration in Swiss albino mice, representing the inaugural comprehensive exploration of the toxicological and safety profiles of C. militaris. Materials and methodsPrior studies have predominantly focused on its biological activities rather than its toxicity. Acute oral toxicity study was conducted at 500, 1000, and 2000 mg/Kg B.W. doses of C. militaris over a 14-day period. For sub-acute toxicity study, three groups of mice were administered 100, 300, and 600 mg/Kg B.W. of C. militaris extract for 28 consecutive days; one group served as a control. Mice were monitored for their body weight and behavioural changes once daily. Hematological, serum biochemical, histopathological, histomorphometric, seminal parameters, and mutagenic investigations were performed post-treatment period. ResultsAcute oral toxicity study at 2000 mg/Kg revealed no signs of toxicity, with an LD50 value surpassing 2000 mg/Kg. No occurrences of mortality observed, and no significant changes were noted in body weight, organ weight, or behaviour. Hematological analysis illustrated a marked upsurge in RBC, Hb, HCT, PLT, MPV, and PCT, alongside minor variations in differential leucocyte count post 28-day treatment. Liver enzyme tests indicated slight elevation in ALP, while renal enzyme tests showed alterations in CRE and BUN levels. Genotoxicity profile and histopathological assessments of the liver, spleen, testis, and ovary manifested no remarkable irregularities, except for mild renal toxicity. Seminal parameters including sperm concentration, motility and testosterone levels demonstrated a noteworthy increase. ConclusionsThe study sheds light on the potential risks and safety considerations associated with C. militaris-based medicinal products. These findings establish a foundation for further investigations and the refinement of dosage optimization in the application of C. militaris, with the aim of mitigating any potential adverse effects.

Impact of polyethylene nanoplastics on human intestinal cells

Polyethylene (PE) is one of the most widely used plastics in the world. Its degradation leads to the production of small particles including microplastics and nanoplastics (NPs). Plastic particles’ presence poses a health risk. The aim of this work was to investigate the toxicity of two model surfactant-free PE NPs prepared by polymerization of ethylene from cationic and anionic water-soluble initiators on human cell lines Caco-2 and HT29-MTX. After physicochemical characterization, their acute and subacute toxicity profile, including cytotoxicity, oxidative stress, and genotoxicity, was evaluated on both cell lines. Results showed a size increase of PE NPs in culture medium. Zeta potential values close to −10 mV were no longer dependent on the initiator charge after adsorption of serum components in culture medium. However, the cellular toxicity of the cationic and anionic PE NPs was very different. A time-and-concentration dependent cytotoxic, oxidative, and genotoxic effects on Caco-2 cells were only observed for PE NPs prepared with cationic initiators. No toxicity was observed on HT29-MTX, likely due to the protective mucus layer. Genotoxicity correlated with oxidative stress of some PE NPs on Caco-2 cells was observed from a concentration of 0.1 mg.mL−1 after 48-h exposure.

Acute and subacute toxicity profiling of methanol extract of Combretum dolichopetalum leaf in albino rats

The leaf of Combretum dolichopetalum is widely used in ethnomedicine to treat cases of diarrhea, inflammation and open wound. This study was hence, undertaken to determine the acute and subacute toxicity profile of the plant in albino rat model. A total of 426 g of dried and pulverized leaves of the plant was extracted with 2.5 L of 80% methanol by cold maceration method. A modified up-and-down method was employed for the oral acute toxicity study, with the extract administered at a maximum dose of 4000 mg/kg. For the subacute toxicity study, 18 rats were assigned into 3 groups (n = 6). Group A (control) received distilled water (5 ml/kg), while groups B and C were given 200 and 400 mg/kg of the extract, respectively. All the treatments were delivered orally for 28 days, after which blood samples were collected for hematology and serum biochemistry. Some vital organs were harvested for histological examination. The result of the acute toxicity recorded neither death nor morbidity even at the highest dose of the extract. For the subacute study, 400 mg/kg of the extract caused significant (p < 0.05) increase in total cholesterol and low density lipoprotein cholesterol (LDL-C); and significant (p < 0.05) decrease in high density lipoprotein cholesterol (HDL-C) in the treated rats when compared with the control. The histology slides showed only a mild fatty infiltration of hepatocytes in the group treated with 400 mg/kg of the extract. The results indicate a high safety index of the Combretum dolichopetalum leaf, however prolonged administration of high doses may cause hyperlipidemia.

Testing of acute and sub-acute toxicity profile of novel naproxen sodium nanoformulation in male and female mice

Nanodrugs offer promising alternatives to conventionally used over the counter drugs. Compared to its free form, therapeutic benefits, and gastric tissue safety of naproxen sodium nanoformulation (NpNF) were recently demonstrated. Essential regulatory safety data for this formulation are, however, not available. To address this, male and female BALB/c mice were subjected to acute and 14-day repeated-oral dose assessments. Our data indicate that NpNF was well tolerated up to 2000 mg/kg b.w. A 14-day subacute toxicity testing revealed that the oral administration of low dose (30 mg/kg) NpNF did not produce any adverse effects on blood profile and serum biochemical parameters. Levels of oxidative stress markers and antioxidant enzymes neared normal. Histology of selected tissues also showed no evidence of toxicity. In contrast, a ten-fold increase in NpNF dosage (300 mg/kg), demonstrated, irrespective of gender, mild to moderate toxicity (p < 0.05) in the brain, stomach, and heart tissues, while ROS, LPO, CAT, SOD, POD, and GSH levels remained unaffected in the liver, kidney, spleen, testis, and seminal vesicles. No effect on serum biochemical parameters, overall indicated a no-observed-adverse-effect level (NOAEL) is 300 mg/kg. Further increase in dosage (1000 mg/kg) significantly altered all parameters demonstrating that high dose is toxic.

Acute and subacute toxicity profile of ethanolic stem bark extract of Albizia coriaria Welw. ex Oliv. in Wistar albino rats

Albizia coriaria (Fabaceae) crude extracts are key ingredients of several licensed and unlicensed herbal products in East Africa. However, there is limited and often contradicting information regarding its toxicity. We therefore evaluated the acute and subacute toxicity of the ethanolic stem bark extract of A. coriaria in mature healthy Wistar albino rats following Lorke’s method and OECD guidelines 407. The LD50 of the ethanolic stem bark extract of A. coriaria was 2000 mg/kg. The acute toxicity signs observed included piloerection, hyperventilation, lethargy, and loss of righting reflex. There was a significant increase in aspartate aminotransferase, alkaline phosphatase, red blood cells and haemoglobin in rats after 28 days at the dose of 500 mg/kg. Histological analyses revealed multifocal random parenchymal necrosis and scattered periportal mononuclear inflammatory cells infiltration in the liver, interstitial nephritis in the kidney and multifocal lymphoid accumulation in the peribronchiolar and perivascular lung tissue at 500 mg/kg. The ethanolic stem bark of A. coriaria was therefore moderately toxic to the rats when administered in a single high oral dose within 24 h. The extract caused a dose dependent toxicity with significant damage to the kidney, liver and lung tissues at a dose of 500 mg/kg after 28 days. Herbal medicines containing A. coriaria extracts should be consumed cautiously due to likelihood of toxicity particularly at higher doses greater than 500 mg/kg.

P26-12: Acute and subacute toxicity profiles in Sprague-Dawley (SD) rats treated with Piperis Longi Fructus (PLF) aqueous extract

P26-12: Acute and subacute toxicity profiles in Sprague-Dawley (SD) rats treated with Piperis Longi Fructus (PLF) aqueous extract

P26-11: Acute and subacute toxicity profiles in Sprague-Dawley (SD) rats treated with Piperis Longi Fructus (PLF) Powder

P26-11: Acute and subacute toxicity profiles in Sprague-Dawley (SD) rats treated with Piperis Longi Fructus (PLF) Powder

Novel Cellular and Immunotherapy: Toxicities and Perioperative Implications.

Targeted cellular and immunotherapies have welcomed a new chapter in multi-modal cancer therapy. These agents harness our innate immune system and destroy malignant cells in a precise way as compared with "legacy" chemotherapeutic agents that largely rely on abolishing cell division. New therapies can augment the T-cell recognition of tumor antigens and effectively prevent tumor cells from their historically successful ability to evade immune recognition. These novel agents cause acute and chronic toxicities to a variety of organ systems (enteritis, pneumonitis, hypophysitis, and hepatitis), and this may masquerade as other chronic illnesses or paraneoplastic effects. As the perioperative footprint of cancer patients increases, it is essential that perioperative providers-anesthesiologists, surgeons, nurse anesthetists, and inpatient hospital medicine providers-be up to date on the physiologic mechanisms that underlie these new therapies as well as their acute and subacute toxicity profiles. Immunotherapy toxicity can significantly impact perioperative morbidity as well as influence perioperative management, such as prophylaxis for adrenal insufficiency, preoperative pulmonary assessment, and screening for thyroid dysfunction, among others.

Assessment of the acute and subacute toxicity of the aqueous extract of Moroccan Ferula communis fruit in a mouse model

Ferula communis L. is thought to possess a wide range of therapeutic qualities. This plant's safety is critical regarding its potential uses as a medicine. Using the techniques outlined in the OECD recommendations, the present study aimed to assess the acute and subacute toxicity profiles of Ferula communis aqueous extract (FC-Ext) in mice. In the acute study, the FC-Ext was administered to adult male and female Swiss albino mice through oral and intraperitoneal routes at doses of 0–4 g/kg. The general behavioral effects, mortality rates, and latency of mortality were evaluated for a period of 14 days. For the sub-acute dose study, the FC-Ext was administered orally to adult mice at doses of 125, 250, and 500 mg/kg on a daily basis for 28 days. Body weight and selected biochemical and hematological parameters were measured, and histological examinations of the liver, kidney, and spleen were conducted to assess any signs of organ damage at the end of the treatment period. The results of the acute toxicity study demonstrated that the LD50 values for the oral and intraperitoneal administration of FC-Ext were 3.6 g/kg and 2.3 g/kg, respectively. In the subacute toxicity study of FC-Ext, no significant changes in body weight were observed. However, a substantial increase in the weights of the liver, kidney, and spleen was observed in male mice. The administration of FC-Ext to mice at doses higher than 250 mg/kg resulted in a decrease in white blood cells and platelets in both sexes and a reduction in red blood cells and mean corpuscular hemoglobin concentration in males and hemoglobin in females. No changes in biochemical parameters were observed. Microscopic examination of vital organs such as the liver, kidney, and spleen revealed no significant injuries. Based on the current results, the aqueous extract of Ferula communis has low toxicity. These findings provide important information about the toxicity profile of the traditional medicine plant Ferula communis.

Acute and Sub-Acute Toxicity Studies of Starch Hyaluronate in Wistar Rats

Starch hyaluronate is used as a new superdisintegrating agent in the development of fastdissolving tablets of poorly soluble drugs because of its improved biocompatibility, and hydrophilicity. The purpose of this study is to assess the acute and subacute toxicity profiles of starch hyaluronate in Wistar rats. The starch hyaluronate was administered to Wistar rats in a single-dose acute study and monitored for seven days. Wistar male and female rats were used in a 28-day sub-acute study that examined the effects of oral doses of 100, 200, and 600 mg/kg body weight/day on body weight, food intake, mortality, biochemical analysis, and histopathologicalevaluation. An acute study revealed that the synthesized starch hyaluronate minimal oral fatal dose for rats was larger than 2000 mg/kg body weight. The subacute toxicity evaluation found no significant changes when compared to the control group, and there was no change in hematological or biochemical parameters. The weights of the liver, kidneys, and pancreas remained constant. Based on the findings, it was concluded that starch hyaluronate at 600mg/kg body weight/day was neither immunogenic nor sensitizing, and was not a reproductive or developmental toxicant, implying that it is relatively safe when taken orally.&nbsp

Evaluation of acute and subacute toxicity of the extract of allium cepa peels in Wistar rats

Allium cepa is a popular recipe used traditionally to treat diabetes, cardiovascular diseases, cancer, wounds and infections. The acute and sub-acute oral toxicity profile of the aqueous extract of Allium cepa peels were evaluated in wistar albino rats in order to determine its safety for use in traditional medicine. The median lethal dose (LD 50) body weights and food intake were determined. The acute oral toxicity of the peel extract was carried out in accordance with OECD guideline 423. In addition to this, the haematological parameters and biochemical parameters were also evaluated. The median lethal dose (LD 50) of the extract was greater than 2000 mg/kg when given orally in the rats. There were no significant changes in the level of haematological parameters and biochemical parameters. The results from this study indicates that Allium cepa peels are relatively safe, hence supporting its use in traditional medicine.

Acute and Sub-acute Oral Toxicity Profile of Root Bark Methanol Extract of Carissa Edulis Vahl

Acute and Sub-acute Oral Toxicity Profile of Root Bark Methanol Extract of Carissa Edulis Vahl

Preclinical In vivo Acute Toxicity Testing of Hyrdoethanolic Extracts of Ficus thonningii Blume (Moraceae) on Wistar Rat Models

Ficus thonningii (Blume) is a medicinal plant whose biological activity has been demonstrated in the management of peptic ulcers. Unfortunately, little scientific data exists on its toxicity. This is why we studied the acute and subacute oral toxicity profile of the hydro-ethanolic extract of the bark of F. thonningii and the systemic exposure of the bioactive components of the plant.Acute toxicity was assessed by administering a single dose of 2000 mg/kg to 5-week-old rats and observed for 14 days. The subacute toxicity study was performed in 6-week-old rats. Animals were orally treated with a daily dose of 125 mg/kg, 250 mg/kg, 500 mg/kg extract for 28 days. A satellite group of animals received 500 mg/kg per day for 28 days and after discontinuation of treatment they were observed for 14 days. Hematological and blood biochemical parameters, as well as kidney and liver histology, were recorded at the end of each experiment. For acute treatment, administration of a dose of 2000 mg/kg did not induce any critical behavioral changes or deaths. Following subacute dosing, biochemical analysis revealed a slight elevation of liver parameters in the 500mg/kg hydroethanolic extract, while no significant increase was observed for kidney parameters. This study showed that daily administration of Ficus thonningii (Blume) for 28 days resulted in the potential to reverse impairment of liver function.

Assessment of the Acute and Subacute Toxicity Profile of Diethylether Extract of Jatropha Tanjorensis leaf in Male Wistar Rats

The aim of study was to evaluate the acute and subacute toxicity of diethylether extract of Jatropha tanjorensis leaves in male Wistar rats. The acute toxicity study was conducted by placing Wistar albino rats into four groups of five rats each. These were sequentially dosed one at a time, using four graded doses of 1250, 2500, 3750 and 5000 mg/kg b.wt of the plant extract. Observations were made and recorded systematically daily for a seven days period for mortality, breathing and other behavioral changes after dose administration. For the sub-acute toxicity study, the animals were divided into ten groups of five rats each. Group 1 served as normal control and received distilled water for a 42 days period. Groups 2,3 and 4 received 167mg/kg b.wt of the plant extract for 14 days, 28 days and 42 days respectively. Groups 5, 6 and 7 received 250mg/kg b.wt of the plant extract for 14 days, 28 days and 42 days period respectively. Groups 8, 9 and 10 received 500mg/kg b.wt of the plant extract for 14, 28 and 42 days respectively. The animals were sacrificed at 14 days interval and the hematological and biochemical parameters were determined. There was a significant (p &lt;0.05) increase in RBC, Hb, HT, MCV and MCH and a decrease in WBC for 14, and 28 days treatment. There was significant increase in the levels of TP, ALB and a decrease in the levels of TBIL, ALP while ALT and AST remained unchanged. The serum markers of lipid profile, total–CHOL, LDL-CHOL, VLDL-CHOL and TG were significantly reduced and HDL-CHOL level was increased. The activities of the stress enzymes GPx, CAT and SOD were increased while the level of MDA was reduced. The K+ level was unchanged, Na+, Cl- and HCO3- levels increased while urea and creatinine levels were decreased. Jatropha tanjorensis diethylether extract elicited non toxic effect in the hematological and biochemical parameters. Thus, the extract can be considered safe for oral administration.

Sub-Acute Toxicological Evaluation of Methanol Leaf Extract of Nymphaea lotus Linn (Nymphaeceae) in Wistar Rats

Nymphaea lotus has been used for centuries as an astringent, aphrodisiac, sedative, analgesic, anti-inflammatory, and for the treatment of infectious diseases. This study is aimed at evaluating the subacute toxicity profile of methanol leaf extract of Nymphaea lotus in Wistar rats. Rats were administered the crude methanol leaf extract orally for 28 days at 250, 500, and 1,000 mg/kg. Weekly body weight, food, and water intake were recorded. On the 29th day, the rats were sacrificed, and their hematological and biochemical parameters were assessed, as well as histological examination of the kidney, liver, stomach, and intestine. The extract had no effect on the body weights, relative organ weights, or food and water intakes of the animals. It had no impact on hematological markers at the tested doses (hemoglobin, packed cell volume, red blood cell, white blood cell, mean corpuscular hemoglobin, mean corpuscular volume, and platelets concentration), except for alkaline phosphate, which was significantly (p≤0.01) greater at the 500 mg/kg. There was no significant effect on liver function parameters evaluated (total bilirubin, direct bilirubin, alanine aminotransferase, aminotransferase, and aspartate). Urea, creatinine and chloride levels were significantly elevated (p≤0.01 and p≤0.05) at 250 and 500 mg/kg doses, but not at the 1,000 mg/kg dose. Histopathological evaluation of the liver, kidneys, stomach, and intestine revealed no notable histological abnormalities. Based on the results, the methanol leaf extract of Nymphaea lotus appears to be generally safe when taken orally at these doses.

Oral acute, sub-acute toxicity and phytochemical profile of Brassica carinata A. Braun microgreens ethanolic extract in Wistar rats

Ethnopharmacological relevanceCurrently, there is a remarkable increase in the consumption of microgreens, (young edible vegetables or herbs), as potential nutraceuticals for the management of diseases. Brassica carinata A. Braun is one of the traditional leafy vegetables cultivated in various parts of Sub- Saharan Africa. The plant is revered for its efficacy in the treatment of wounds and gastrointestinal disorders among other medicinal benefits. It is therefore crucial to characterize Brassica carinata microgreens for their phytoconstituents and ascertain their safety for use. Aim of the studyThe study evaluated the oral acute and subacute toxicity of Brassica carinata microgreens ethanol extract (BMEE) in Wistar rats and identification of its chemical composition and profile. Materials and methodsFor acute toxicity (14 days), rats were grouped into four and received a single oral dose, the control group received distilled water, while others received 500 mg/kg, 1000 mg/kg, and 2000 mg/kg of BMEE. For the subacute toxicity (28 days), rats in four groups received daily doses of 250 mg/kg, 500 mg/kg or 1000 mg/kg and distilled water. Daily clinical observations like lethargy and mortality were conducted. Hematological, biochemical, and histopathological evaluations were performed at the end of each experiment. Phytochemical profile was determined using a UV-VIS spectrophotometer and Gas Chromatography coupled to Mass Spectrometry (GC-MS) analysis determined the potential bioactive components in the microgreens extract. ResultsIn both acute and sub-acute toxicity studies, no mortalities, indications of abnormality, or any treatment related adverse effects were observed at doses of 2000 mg/kg, 1000 mg/kg, 500 mg/kg, and 250 mg/kg. The LD50 of BMEE was above 2000 mg/kg. No significant (p > 0.05) changes in the hematological and biochemical parameters of the treated groups compared to the control groups in both studies. Histopathological examination of the liver, kidney, lungs, and heart revealed a normal architecture of the tissues in all the treated animals. Phytochemical analyses revealed the presence of flavonoids (most abundant), phenols and alkaloids. Phytol, linoleic acid, and 9,12,15-octadecatrienoic acid, among other compounds, were identified by GC-MS analysis. ConclusionThe results showed that B. carinata microgreens ethanol extract is nontoxic and found to have several compounds with reported pharmacological significance suggesting safety for use.

Toxicity Profiling and Antihyperuricemic Activity of Goubion: A Polyherbal Formulation.

The objective of the present study was to determine the acute and subacute toxicity profile of a polyherbal formulation called "Goubion" in addition to the in vivo antihyperuricemic study using fructose-induced hyperuricemia. Goubion is a combination of Colchicum autumnale (tuber), Tribulus terresteris (fruit), Vitex negundo (leaves), Smilax chinensis (root), Glycyrrhiza glabra (root), and Curcuma amada (rhizome). The acute toxicity study revealed no signs of mortality and morbidity at a single dose of 2000 mg/kg. Similarly, the results of the subacute repeated dose toxicity study exhibited no signs of mortality at any of the doses. However, significant changes in hematological, biochemical, and renal parameters were recorded at the dose of 60 mg/kg. Antihyperuricemic activity was tested at the dose of 15 mg/kg and 20 mg/kg of Goubion, respectively against 5 mg/kg Allopurinol. Based on the antihyperuricemic study, we infer that the Goubion has a significant hypouricemic action, as it remarkably decreased the elevated uric acid levels. The results also suggest the potential inhibitory capability of Goubion on xanthine oxidase dehydrogenase might be the mechanism behind the hypouricemic effect.