Sub-health Status Research Articles

Stick-and-sensing microneedle patch for personalized nutrition management

The focus of the next-generation healthcare system has moved forward from single disease detection to comprehensive health interpretation, namely, from disease diagnosis to prophylaxis via the early management of sub-health status. Among the technologies, microneedles have emerged as an efficient and powerful tool to realize painless and real-time sensing in the interstitial fluid (ISF), offering promising solutions for dynamic and direct reflection of nutritional profiles. Herein, we present a film-like, multifunctional microneedle patch, that is composed of three laser-induced graphene (LIG) based electrodes for minimally invasive and timely detection of glucose and vitamin C in the ISF. The microneedle patch is simply fabricated with a thickness of ∼0.25 mm polyimide (PI) film by a commercial laser platform, where laser-engraving is operated to form graphene electrodes, and laser-cutting accounts for the needle-tip shaping. Such fast, low-cost, and scalable manufactured microneedle patches showed excellent performance for electrochemical sensing and therefore allowed reliable on-the-tip monitoring of changes in glucose and vitamin C levels. Long-term studies in vivo and demonstrations in planta indicated the great promise of our microneedle patch to revolutionize dietary guidelines based on individualized status for effective nutrition management.

Person-centered exploration of work-related stress patterns, predictors, and their association with subhealth in midwives: A cross-sectional study

BackgroundMidwives experience elevated levels of work-related stress. However, there is limited understanding of the patterns of this stress, its sociodemographic and work-related determinants, and its association with sub-health. MethodThis multi-center cross-sectional study, conducted in 21 hospitals in Zhuhai City, Guangdong Province, China, involved 300 midwives. Work-related stress levels in midwives were evaluated using the Chinese version of the Nursing Stress Scale, while social, physical and mental sub-health status was measured with the Sub-Health Measurement Scale. Utilizing latent profile analysis, the study aimed to categorize midwives into homogeneous profiles based on patterns of work-related stress. ResultsAmong the 300 midwives examined, three distinct profiles were identified: profile 1 (n=57, 19 %), characterized by low work-related stress; profile 2 (n=149, 50 %), representing the moderate work-related stress class; and profile 3 (n=94, 31 %), indicative of high work-related stress. Midwives in the high work-related stress profile tended to be younger, with lower monthly income, lower professional titles, and a higher likelihood of night shift work (all P<0.01). Significant and noteworthy trends were observed in sociodemographic characteristics (age, monthly income, and professional title) and work-related characteristics (night shift status). After controlling for confounders, the work-related stress profile demonstrated a negative association with social, physical and mental sub-health status. ConclusionThis study highlights integrating stress domains and adopting a person-centered approach to examine midwives’ work-related stress. Identifying predictors of profile membership and their relationship with sub-health can inform tailored interventions to reduce stress and improve midwives' well-being.

Enhanced immunity effect of Korean Red Ginseng capsule: A randomized, double-blind and placebo-controlled clinical trial

BackgroundAs a physiological function of body, immunity can maintain health by identifying itself and excluding others. With economic development and increasingly fierce social competition, the number of sub-healthy population is gradually increasing, and the most basic problem exposed is human hypoimmunity. Hypoimmunity can be manifested as often feeling tired, catching colds, mental depression, etc. In order to enhance immunity, eating healthy foods with the effect of enhancing immunity may become an effective choice. KRG has pharmacological effects of enhancing immunity. Because the screening and evaluation method of immune population are not unified, there are relatively few KRG immunity tests for sub-health population. It is of great significance to study the effect of KRG on people with hypoimmunity to improve sub-health status. MethodsThis was a 180-day, randomized, double-blind, placebo-controlled clinical trial. According to the trial scheme design, 119 qualified subjects were included and randomly divided into the test group taking KRG and the placebo control group. Subjects need to check safety indicators (blood pressure and heart rate, blood routine, liver and kidney function, urine routine and stool routine) and efficacy indicators (main and secondary) inspection at baseline, efficacy indicators inspection during the mid-term of the test (90th days of administration), safety and efficacy indicators inspection after the test (180th days of administration). ResultsAfter the test, the safety indicators of placebo control group and KRG test group were basically within the normal range, and there is no significant difference in fireness score between the two groups. Through follow-up interviews, it was found that the subjects in the test group and the control group had no adverse reactions and allergic reactions such as nausea, flatulence, diarrhea, and abdominal pain during the test period. Self-comparison of the test group, the results of the main efficacy indicators: (1) immune related health scores were significantly improved in the mid-term and after the test (P < 0.01), (2) CD3 and CD4/CD8 increased significantly after the test (P < 0.05), (3) IgG, IgA, IgM and WBC increased significantly in the mid-term and after the test (P < 0.01); the results of the secondary efficacy indicators: (1) TNF-α decreased significantly in the mid-term (P < 0.05), IFN-γ decreased significantly in the mid-term (P < 0.01), (2) NK increased significantly in the mid-term and after the test (P < 0.05), (3) monocyte increased significantly in the mid-term and after the test (P < 0.01). Inter-group comparison of the test group and the control group, the results of the main efficacy indicators: (1) immune related health scores were higher than that of the control group in the mid-term and after the test (P < 0.01), (2) IgA of the test group was higher than that of the control group in the mid-term and after the test (P < 0.05); the results of the secondary efficacy indicators: (1) WBC of the test group was higher than that of the control group in the mid-term (P < 0.05); (2) monocytes of the test group were higher than that of the control group in the mid-term and after the test (P < 0.05), neutrophils of the test group were higher than that of the control group in the mid-term (P < 0.05). ConclusionTaking KRG has no adverse effects on the health of the subjects. According to the standard of clinical trial scheme, the immune related health scores and IgA in the main efficacy indicators were positive, which shows that KRG is helpful in enhancing human immunity.

Exploring the Experiences and Support of Nurses as Second Victims After Patient Safety Events in China: A Mixed-Method Approach.

To investigate the current status of experience and support of nurses as second victims and explore its related factors in nurses. A sequential, explanatory, mixed-method study was applied. A total of 406 nurses from seven tertiary hospitals in China were chosen as participants between September to October 2023. The Chinese version of the Second Victim Experience and Support Questionnaire (SVEST), Somatic Complaints of Sub-health Status Questionnaire (SCSSQ) and Generalized Anxiety Disorder (GAD-7) were applied to collect quantitative data. Eight nurses were selected for a qualitative study through in-depth interviews. Through interpretive phenomenological analysis, the interview data were analysed to explore the experience and support of nurses as second victims. Practice distress (15.74±4.97) and psychological distress (15.48±3.74) were the highest dimensions, indicating Chinese nurses experienced second victim-related practice and psychological distress. Nurses with different gender, age, education, marital status, income, working hours, professional titles, and unit types have different levels of second victim-related experience and support (p <0.05). In addition, the score of SVEST was positively associated with SCSSQ (r =0.444) and GAD-7 (r =0.490) (p <0.05). This qualitative study found that the experience and support of nurses as second victims included nurses' perceptions and needs for patient safety events; psychological, physical and practice distress of nurses; and nurses and hospitals coping style after patient safety events. Our findings suggest that nurses who are second victims of patient safety events experience severe practice and psychological distress, indicating that nursing managers should pay attention to psychological and practice distress of nurses after patient safety events and provide effective preventive measures.

The intervention of consumer product design optimization incorporating color psychology on consumers’ anxiety disorders

BackgroundAny product design cannot do without color design, and color is also an important form of psychological treatment for anxiety disorders. This study is based on color, optimizing product design, intervening, and treating consumer anxiety disorders, and combining conventional medical methods to improve consumers’ sub-health status and generate positive social effects.Subjects and MethodsWe performed a MEDLINE search focusing on the past 10 years. Keywords used were attachment, neurobiology, and psychotherapy. We included original studies and existing reviews looking at all types of formal psychotherapy used and focusing on human research. Exclusion criteria were non-psychotherapeutic interventions and attachment based on couples only.ResultsThe experimental results showed that the number of people who observed red light was greater than that of those who observed blue light, and the number of people who observed green and blue light was 21,25. The order of changing colors has little effect on the experimental results. The level of activity in brain regions varies with color, with darker red areas indicating more active brain activity, while darker blue areas indicating less active brain activity.ConclusionsRed light makes the brain tense and excited, while blue light makes the brain calm and relaxed. Design products with colors, expressing emotions through color combinations on the screen. Faced with the psychological dilemma of anxiety, through the application of color in product design, color optimization is carried out to explore effective color application solutions that can be suitable for various psychological needs.

Analysis of animation screenwriter’s anxiety and intervention countermeasures

BackgroundAnimation has special artistic creation rules, so animation writers often suffer from anxiety disorder because of excessive pressure when creating animation scripts. This study from psychological quality and physical health, analyzes the sources of patients’ anxiety, and intervenes their anxiety treatment by using group psychological counseling with trait hope scale.Subjects and MethodsIn this study, 60 animated writers with anxiety disorder were selected on different platforms of social media and were divided into experimental group and control group. In the experimental group, 30 writers received the intervention of group psychological counseling with hope trait, while in the control group, 50 patients did not receive treatment. The study used the Hope Trait Scale and Sarason Test Anxiety Scale (TAS) to measure, and compared the hope trait scores of the two groups with TAS scores after 8 weeks of treatment.ResultsThe study utilized SPSS 23.0 statistical software to analyze the data. The hope trait scores and HAMA scores of the experimental group were (163.4±20.2) and (13.5±2.7) after 8 weeks, and those of the control group were (138.5±19.3) and (17.1±2.3) after 8 weeks, which were better than those of the control group, and the comparison between the two groups showed significant differences (P&lt;0.05).ConclusionsScript stress and physical sub-health status are the main sources of anxiety symptoms in animation writers, and the study utilized hope trait group counseling to effectively alleviate anxiety symptoms in animation writers.

Ginseng Extract Improves the Sub-health Status in Young Population: An Observational Study

Ginseng Extract Improves the Sub-health Status in Young Population: An Observational Study

Relationship between depression and lifestyle factors in Chinese adults using multi-level generalized estimation equation model.

Relationship between depression and lifestyle factors in Chinese adults using multi-level generalized estimation equation model.

The correlation between physical inactivity and students' health based on data mining and related influencing factors.

The sedentary behavior among college students has become one of the most important factors affecting the development of physical and mental health. Chronic lack of physical activity may lead to health problems such as decreased physical fitness, and increased psychological disorders. In the post-epidemic era, it is necessary for college students to have a strong immune system, and a strong body cannot be achieved without regular leisure physical activity. Therefore, it is necessary to explore the relationship between relevant health factors and physical activity. This paper presents an optimized COM-B model. And the experimental results show that the optimized model is well applied in describing the current situation of physical activity participation among college students, analyzing the distribution characteristics of socio-demographic variables related to physical activity, and exploring the correlation between physical activity and the subhealth status of college students.

Nanostructured steady-state nanocarriers for nutrients preservation and delivery.

Food bioactives possess specific physiological benefits of preventing certain diet-related chronic diseases or maintain human health. However, the limitations of the bioactives are their poor stability, lower water solubility and unacceptable bioaccessibility. Structure damage or degradation is often found for the bioactives under certain environmental conditions like high temperature, strong light, extreme pH or high oxygen concentration during food processing, packaging, storage and absorption. Nanostructured steady-state nanocarriers have shown great potential in overcoming the drawbacks for food bioactives. Various delivery systems including solid form delivery system, liquid form delivery system and encapsulation technology have been developed. The embedded food nutrients can largely decrease the loss and degradation during food processing, packaging and storage. The design and application of stimulus and targeted delivery systems can improve the stability, bioavailability and efficacy of the food bioactives upon oral consumption due to enzymatic degradation in the gastrointestinal tract. The food nutrients encapsulated in the smart delivery system can be well protected against degradation during oral administration, thus improving the bioavailability and releazing controlled or targeted release for food nutrients. The encapsulated food bioactives show great potential in nutrition therapy for sub-health status and disease. Much effort is required to design and prepare more biocompatible nanostructured steady-state nanocarriers using food-grade protein or polysaccharides as wall materials, which can be used in food industry and maintain the human health.

Research on sub-health monitoring of equipment based on industrial big data technology

Aiming at the problems of traditional equipment fault diagnosis, such as poor real-time performance, low efficiency and strong subjectivity, a method of industrial equipment sub-health status monitoring based on big data technology was proposed. Firstly, the characteristic engineering of vibration signals is formed by preprocessing vibration signals collected by sensors located near the bearing shaft. Then, the deep residual shrinkage network DRSN was used to build the classification model. By tuning the model parameters, the optimal sub-health status identification model of industrial equipment was obtained. Finally, the accuracy, robustness and generalization of the model were verified. Experimental results show that this method has better generalization performance and can significantly improve the accuracy of industrial equipment fault diagnosis, which has important theoretical value and practical significance to solve the sub-health problems of industrial equipment.

ANTI-FATIGUE EFFICACY OF CROCIN IN MICE VIA REGULATION OF NRF-2/HO-1 PATHWAY-MEDIATED OXIDATIVE STRESS

ABSTRACT Introduction: Fatigue is a manifestation of sub-health status, which causes serious negative effects in daily life. The antioxidant properties of crocin have been widely investigated in many diseases. However, no correlation between crocin and fatigue was reported. Objective: To verify the anti-fatigue efficacy of crocin in swimming-induced exhaustive time. Materials and Methods: In this study, mice were treated with crocin for 28 days to assess its anti-fatigue efficacy. Exhaustive time, fatigue-relevant biochemical indices, inflammatory cytokines, energy metabolism indicators, oxidation parameters, and the NRF-2/HO-1 pathway were analyzed to explore evidence of crocin in high-intensity exercise. Results: Our research indicated that crocin dramatically extended swimming-induced exhaustive time. In strenuous swimming, crocin clearly eliminated BLA and SUN to maintain internal environment homeostasis, while it markedly improved glycogen concentrations in the muscles and liver to promote energy reserves. Moreover, crocin visibly improved the inflammatory reaction, as represented by reductions in TNF-a and IL-6, promoting endurance capacity. In the muscles, crocin noticeably enhanced SDH and Na+K+-ATP activities to improve energy metabolism in strenuous swimming. Lastly, crocin markedly improved SOD and CAT activities via the NRF-2/HO-1 pathway to defend against oxidative stress-induced fatigue. Conclusions: Crocin provides oxidation resistance and can be developed into anti-fatigue nutriments. Evidence level II; Comparative prospective study.

Comment on "Prevalence of subhealth status and its effects on mental health and smartphone addiction: a cross-sectional study among Chinese medical students".

Comment on "Prevalence of subhealth status and its effects on mental health and smartphone addiction: a cross-sectional study among Chinese medical students".

Quantitative Proteome Analysis Reveals Melissa officinalis Extract Targets Mitochondrial Respiration in Colon Cancer Cells.

Melissa officinalis (MO), known as lemon balm, is a popular ingredient blended in herbal tea. In recent decades, the bioactivities of MO have been studied in sub-health and pathological status, highlighting MO possesses multiple pharmacological effects. We previously showed that hot water MO extract exhibited anticancer activity in colorectal cancer (CRC). However, the detailed mechanisms underlying MO-induced cell death remain elusive. To elucidate the anticancer regulation of MO extract in colon cancer, a data-driven analysis by proteomics approaches and bioinformatics analysis was applied. An isobaric tandem mass tags-based quantitative proteome analysis using liquid chromatography–coupled tandem mass spectrometry was performed to acquire proteome-wide expression data. The over-representation analysis and functional class scoring method were implemented to interpret the MO-induced biological regulations. In total, 3465 quantifiable proteoforms were identified from 24,348 peptides, with 67 upregulated and 54 downregulated proteins in the MO-treated group. Mechanistically, MO impeded mitochondrial respiratory electron transport by triggering a reactive oxygen species (ROS)-mediated oxidative stress response. MO hindered the mitochondrial membrane potential by reducing the protein expression in the electron transport chain, specifically the complex I and II, which could be restored by ROS scavenger. The findings comprehensively elucidate how MO hot water extract activates antitumor effects in colorectal cancer (CRC) cells.

Semen raphani weakened the action of ginseng under chronic fatigue condition

Ethnopharmacological relevanceFatigue is a kind of subhealth status and people paid much more attention on it. Ginseng is used to treating fatigue as a kind of qi -tonifying drug in Chinese medicine. In the traditional applications, there is a viewpoint that ginseng could not be used with semen raphani and supposed that semen raphani is a kind of qi regulating drug, which will reduce the qi invigorating effect of ginseng. However, the underlying combination mechanism of the two drugs remained unclear. Aim of the studyThe aim of this study is to explore whether ginseng can be used with semen raphani or not to remedy acute and chronic fatigue conditions. MethodsWe used normal and weight-bearing swimming method combined with appetite control animals. The biochemical indexes in energy metabolism, antioxidant, regulating endocrine system and immunity capacities were performed to explore the antagonism effect of semen raphani on ginseng under acute and chronic fatigue conditions. The serum and urine metabolomics were investigated using liquid chromatography quadrupole time-of-flight mass spectrometry (LC-QTOF/MS). Fecal flora was analyzed via 16S rRNA amplicon sequencing. ResultsThe combination of ginseng with semen raphani have no influence on acute fatigue effect compared with ginseng alone. Both can improve the exhausted swimming time, the activity of GSH-Px, LDH and Na+-K+-ATPase. Furthermore, the combination of ginseng with semen raphani can increase the urine volume of rats and down-regulate the content of AQP-3, which can alleviate the “fireness” side-effect of ginseng. But the abundance and diversity of bacterial are decreased under acute fatigue experiment. Both the combination of ginseng with semen raphani and ginseng alone can remedy chronic-fatigue. They can also regulate the endocrine system, immune system, citric acid cycle metabolism, tryptophan metabolism, fatty acid metabolism, glycolysis/gluconeogenesis, etc. Furthermore, they can promote substance metabolism and energy metabolism in qi deficiency rats, and increase the abundance and diversities of the flora. While with the increased content of semen raphani, the combination of ginseng and semen raphani weaken the capacity of antioxidant, lactic acid metabolism, energy metabolism, flora diversity and regulation of endocrine system. ConclusionCompared with ginseng alone, the combination of ginseng with semen raphani can weaken the qi invigorating ability under chronic fatigue condition. The more ratios of semen raphani is in the combination of the two drugs, the less the power of treating chronic fatigue is. Compared with ginseng alone, the combination of ginseng with semen raphani have no influence on the qi invigorating ability under actue fatigue experiment. But the combination of ginseng with semen raphani will benefit for the “fireness” side-effect of ginseng.

Prevalence of subhealth status and its effects on mental health and smartphone addiction: a cross-sectional study among Chinese medical students.

This study aimed to investigate the suboptimal health status or subhealth status and their relationship with mental health and smartphone addiction among Chinese medical students. A cross-sectional survey was conducted at Wannan Medical College of China in Wuhu. A total of 2,741 students were surveyed in October 2020. Of 2,741 Chinese medical students who completed the survey, 904 (33%) participants reported to have had subhealth status. Anxiety status (p<0.001), depression status (p<0.001), and smartphone addiction status (p<0.001) have strong association with subhealth status. This survey shows that the detection rate of subhealth status in Chinese medical students was 33%. Anxiety, depression, and smartphone addiction students had a higher detection rate of subhealth status. The anxiety, depression, and smartphone addiction of Chinese medical students are associated with subhealth status.

海洋生物多样性损害与沿海地区经济增长关系实证研究

PDF HTML阅读 XML下载 导出引用 引用提醒 海洋生物多样性损害与沿海地区经济增长关系实证研究 DOI: 10.5846/stxb202105141269 作者: 作者单位: 作者简介: 通讯作者: 中图分类号: 基金项目: 国家社会科学基金重大项目（16ZDA049） Empirical research on the relationship between marine biodiversity damage and economic growth in coastal areas, China Author: Affiliation: Fund Project: 摘要 | 图/表 | 访问统计 | 参考文献 | 相似文献 | 引证文献 | 资源附件 | 文章评论 摘要:海洋生物多样性是海洋生态系统服务的基础，保护海洋生物多样性不仅对维持地球生态系统的功能至关重要，也与人类福祉密切相关。基于沿海11个省区生态系统亚健康程度指标和物种多样性损害指标，运用面板回归模型对中国沿海地区经济增长与海洋生物多样性损害的关系进行实证考察。研究结果表明：（1）海洋生态系统亚健康程度与沿海地区经济增长之间存在显著的线性关系，随着经济增长，典型海洋生态系统亚健康状态占比呈现出持续上升趋势。此外，实施排污费制度和建立海洋自然保护区有利于抑制海洋生态系统的恶化。（2）海洋物种多样性损害与沿海地区经济增长之间存在显著"倒U"型关系，随着经济增长，海洋物种多样性损害呈现先上升后下降的态势，转折点为人均GDP 45145元，目前海南省、广西壮族自治区、河北省未跨过转折点。此外，排污费制度有利于抑制海洋物种多样性损害，而沿海地区目前的产业结构加重了海洋物种多样性损害。根据实证分析结果，海洋生态系统健康尚未出现拐点，沿海地区经济增长如果建立在对生态环境破坏的基础上，则势必会造成生物多样性的损害。因此从规范海域利用方式，完善海岸生态保护红线划定，加强生态系统的监测与管理，保持绿色可持续的经济发展方式，引导沿海城市紧凑发展等方面提出保护和管理海洋生物多样性的建议。 Abstract:Marine biodiversity is fundamental to the provision of Marine ecosystem services. Protecting Marine biodiversity is not only essential to the functioning of the earth's ecosystems, but also closely related to human well-being. Based on the indicators of Marine ecosystem health and species diversity damage in China's coastal provinces, this paper use panel regression model to analyze the relationship between economic growth and Marine biodiversity damage in China's coastal areas. The results showed that:(1) there is a significant linear relationship between the sub-health degree of Marine ecosystems and the economic growth in coastal areas. With the economic growth, the sub-health status of typical Marine ecosystems has a continuous upward trend. In addition, the implementation of pollution charge system and the establishment of Marine nature reserves are conducive to suppress the deterioration of Marine ecosystem. (2) There is an inverted U-shaped relationship between the damage of Marine species diversity and the economic growth of coastal areas. The turning point was 45,145 yuan per capita GDP. With economic growth, the damage of Marine species diversity increase first and then decrease. From the perspective of time trajectory, the inflection point of species diversity damage corresponds to the time stage from 2009 to 2010. From the perspective of spatial distribution, Hainan province, Guangxi Zhuang Autonomous Region and Hebei Province are still in the rising part of the curve, The economic growth level of Shandong, Liaoning, Shanghai, Zhejiang, Tianjin, Jiangsu, Guangdong and Fujian provinces has passed the inflection point value, those provinces are in the curve decline stage. The pollution charge system is beneficial to suppress the Marine species diversity damage, but the current industrial structure in coastal areas aggravate the Marine species diversity damage. According to the results of empirical analysis, we know that if the economic growth is based on the destruction of the ecological environment, it is bound to cause damage to biodiversity. Even though there is an inverted U-shaped relationship between Marine species diversity and economic growth in coastal areas, there is still no inflection point between economic growth and Marine ecosystem health, so the sub-health of Marine ecosystem may further lead to the loss of Marine biodiversity, forming a vicious circle. some suggestions are put forward to protect and manage Marine biodiversity:(1) we should strengthen biodiversity protection, broaden the ways of Marine biodiversity protection, and ensure the diversity of Marine biodiversity protection methods;(2) we should maintain a green and sustainable pattern of economic development and promote industrial restructuring and upgrading. meanwhile, we should guide the smart growth of coastal areas and prevent the occupation of coastal wetlands and large-scale reclamation of land due to blind pursuit of economic growth. 参考文献 相似文献 引证文献

The psychology strategies for treating male patients with andrology diseases

With the development of social economy, the biological-psychological-social medical model is gradually formed to treat andrology diseases. The problem of male reproductive and mental health is one of focuses in the medical community. Paying attention to the sub-health status and the quality of life is the direction of the development and effort of andrology science in future. The studies on the relationship between the psychological factors and male reproductive health can help to solve some problems related to the male's health. In this paper, the psychology strategies are discussed for treating male patients with andrology diseases.

Feasibility Research on the Application of Facial Expression Recognition in College Students' Mental Health Interview

Using computer expression recognition technology to learn the different reflections of mental sub-health status, and then explore the possibility of using facial expression recognition method in new interviews to identify mental sub-health status through this method. Methods the sub-health self-assessment scale and symptom checklist 90 (SCL-90) were used for questionnaire survey. Twenty-one subjects were selected and divided into experimental group 1, experimental group 2 and control group through pairing. An experimental study of facial expression feedback was carried out. Results compared with the control group, the somatization, interpersonal sensitivity, anxiety and psychosis of the experimental group 1 were significantly lower than those of the control group (t=2.25, -2.45, -2.42, -2.39; p<0.05); Compared with the control group, the interpersonal sensitivity and anxiety of the experimental group 2 were significantly lower than those of the control group (t=-2 06, -2.16, -2.23; p<0.05); There was no significant difference between experimental group 1 and experimental group 2. Through the computer analysis test of the sampled data, the conclusion shows that the use of computer facial expression recognition can identify the possibility of mental sub-health status of college students.

Individuals with sub-health status have obviously unbalanced structure of the intestinal flora: analysis of 150 nursing staff members

To explore the difference in intestinal flora composition between individuals with sub-health status and healthy subjects. From November, 2020 to May, 2021, a total of 150 nursing staff members in Nanfang Hospital were selected for this study, including 75 participants with sub-health status (SHS group) and 75 healthy participants (control group). Fecal samples were collected from all the participants for analysis of the diversity and species composition of the intestinal flora using high-throughput sequencing for V3-V4 region of 16S rRNA gene. The results of α diversity analysis showed no significant difference in Chao1 index between the two groups (P=0.619), but the Shannon index was significantly higher in SHS group than in the control group (P < 0.001). The results of β diversity analysis showed significant differences in the community structure between the SHS group and the control group (R=0.227, P=0.001). At the phylum level, the intestinal flora in the two groups were composed mainly of Bacteroidota, Firmicutes and Actinobacteriata, and of Prevotella, Bacteroides, Blautia and Faecalibacterium at the genus level. Species difference analysis identified significant differences in the relative abundance between the two groups in 4 phyla, 3 classes, 3 orders, 3 families and 3 genera (P < 0.05). Compared with healthy subjects, the individuals with sub-health status have obviously unbalanced structure of the intestinal flora.