Abstract Introduction Unfamiliar environments are often poorly conducive to adequate sleep, especially for patients within different care settings, where proper sleep is vital for recovery and overall health. This scoping review thus aims to synthesise the evidence around which aspects of the sleep environment are measured within rehabilitation, sub-acute and aged care settings, how they are being measured, and to identify if there are any ‘gold standards’ against which the environmental factor measurements are compared. Method A search was conducted from inception to May 2023 within PubMed, EMBASE, Cochrane Library, Cumulated Index to Nursing and Allied Health Literature (CINAHL), PsycINFO, and Web of Science. Eligibility criteria included full length original studies of any design reporting on the measurement properties of environmental factors affecting sleep of adult patients admitted to rehabilitation/sub-acute wards and aged care facilities. Results This scoping review identified 2185 studies after duplicate removal, of which 82 studies have been admitted for data extraction following review from two authors. Data will be sought for the following variables: publication year, country, study aim, study design, care setting, population characteristics, environmental factors studied, unit of measurement, specific measure, measurement method, gold standard comparators (if any). Discussion It is anticipated that this review will be completed by August 2023 and will identify the most common methods used to measure environmental factors affecting sleep outcomes within the literature. This study will thereby inform and aid researchers and clinicians with developing future environmental interventions and measuring their impact within rehabilitation, subacute and aged care settings.