Preservation Of Structure Research Articles

The Process of Single Surgeon Through Structural and Dorsal Preservation in Primary Rhinoplasty: A 594 Case Series with One-Year Follow-Up

The Process of Single Surgeon Through Structural and Dorsal Preservation in Primary Rhinoplasty: A 594 Case Series with One-Year Follow-Up

The technology of restoration of the red brick facade on the example of an object of cultural heritage of regional significance “Mansion of the Philistine Galaktionov G.I.”

The article provides brief historical and archival information about the object of cultural heritage of regional significance “Mansion of the philistine Galaktionov G.I.”. A description of the decorative solution of the facades of the building, information about its spatial planning solution is given. The emphasis is placed on an unusual design solution of the main facade of the building – the vertical log cabin is lined with brickwork. Some results of complex engineering surveys are presented, from which it can be seen that the preserved structures of the building are in disrepair, there is a large percentage of masonry losses along the facades. However, it is still possible to preserve the original facing layer of the masonry of the main facade and technological solutions for its restoration are given. The description of the measures after the work on the preservation of the red brick facade is given. An assertion is made about the individuality of the developed technology, since each object of cultural heritage is unique from the point of view of the degree of preservation of structures.

Design, synthesis and biological studies of new isoxazole compounds as potent Hsp90 inhibitors.

Heat shock protein 90 (Hsp90), a molecular chaperone, contributes to the preservation of folding, structure, stability, and function proteins. In this study, novel compounds comprising isoxazole structure were designed, synthesized and their potential ability as Hsp90 inhibitors was validated through docking studies. The active site-based compounds were prepared through a multi-step synthesis process and their chemical structures were characterized employing FT-IR, NMR, and mass spectrometry analysis. Cytotoxic and Hsp90 inhibition activities of synthesized compounds were assessed by MTT assay and ELISA kit, respectively. Based on the obtained results, compound 5 exhibited the highest cytotoxicity (IC50; 14 µM) against cancer cells and reduced Hsp90 expression from 5.54 ng/mL in untreated (normal cells) to 1.56 ng/mL in cancer cells. Moreover, molecular dynamics (MD) simulation results indicated its high affinity to target protein and approved its excellent stability which is essential for exerting an inhibitory effect on cancer cell proliferation.

Quantization and the Preservation of Structure across Theory Change

Abstract This article presents a novel sense in which theoretical structure has been preserved across the transition from classical to quantum physics. I import mathematical tools from category theory that have been used for structural comparisons in the context of theoretical equivalence and apply these tools to new situations involving theory change. The structural preservation takes the form of a categorical equivalence between categories of models of classical and quantum physics. I situate the significance of this structural preservation in terms of prospects for theory construction in quantum physics.

Feasibility of Using Inertial Measurement Units (IMUs) to Augment Cadaveric Temporal Training.

Insertional speed of cochlear implant electrode arrays (EA) during surgery is correlated with force. Low insertional speed, and therefore force, may allow for preservation of intracochlear structures leading to improved outcomes. Given the importance of low insertional speeds, we investigate the feasibility of using inertial sensors for kinematic analysis during EA insertion to augment otolaryngology-head and neck surgery training. Practicing otolaryngology surgeons were recruited and inertial measurement units (IMU; Metamotions+, MBIENTLAB Inc, San Jose, CA) consisting of accelerometers were used to measure hand speed during EA (Cochlear™Nucleus®CI522 cochlear implant with Slim Straight electrode, Cochlear Limited, Sydney, Australia) insertion into a cadaveric cochlea. A mixed regression model was utilized to determine differences in speed across trials within a surgeon. A total of nine trials were performed by three surgeons. The highest mean ± SD speed obtained was 8.4 ± 1.7 mm/s, and the highest speed was 22.5 mm/s. Mean speed was not significantly different across trials within surgeons (p > 0.05). IMUs are relatively inexpensive and relatively easy to use sensors that provide information on variables that may be of interest for otolaryngology resident training. The use of IMUs as part of advanced temporal training for cochlear electrode insertion can provide insight into hand speed, thereby allowing residents to train with specific regard to this variable. Future randomized-controlled trials can be carried out to determine whether IMUs are conducive to lower insertional speeds. NA Laryngoscope, 2024.

Factors Contributing to Early Recovery of Urinary Continence Following Radical Prostatectomy: A Narrative Review

Stress urinary incontinence (SUI) is a common condition in patients following radical prostatectomy (RP), which has a significant impact on all aspects of quality of life and is associated with significant social stigma. The factors that improve urinary incontinence in patients following surgery remain controversial. The aim of our narrative review was to identify and synthesise the latest evidence on pre-, intra- and post-operative factors and predictors that contribute to early continence recovery after RP. In this narrative review, primary resources were identified by searching PubMed, EMBASE and Medline, and secondary resources were collected by cross-referencing citations in the relevant articles. We started our review by searching for systematic reviews of factors and predictors that contribute to early recovery of urinary continence after RP. We then reviewed societal guidelines such as the American Urological Association and European Urological Association guidelines on male urinary incontinence. This review focuses on the pre-, intra and postoperative factors that influence postoperative SUI after RP, as well as highlighting modifications in surgical techniques that lead to early continence recovery. Increasing age, higher BMI, shorter membranous urethral length (MUL), and larger PV are independent prognostic factors for SUI within 3 months after RP. Factors such as modified surgical technique preservation of anatomical structure lead to influence postoperative early continence recovery. SUI after RP is influenced by various factors. These factors include not only anatomical landmarks and patient-related factors such as age, BMI, length of MUL and prostate volume, but also prior transurethral resection or laser enucleation of the prostate, the surgeon’s expertise, the surgical approach and NS technique.

A clinical approach for no prep direct composite veneers with opaquer/ tint to reshape a smile: a case report

Dental aesthetic has a profound influence on both self-esteem and social interactions. Patients desire changes in their smiles, while dentists aim for the preservation of dental and adjacent structures, as well as durability. In this context, no-prep resin veneers emerge as a potential solution, as they allow for the preservation of the maximum amount of healthy dental structure, pulp integrity, and dental strength. Nevertheless, treating darkened substrates poses a challenge, and conservative options like composite resins have gained recognition as restorative materials due to their aesthetic qualities and ability to preserve healthy dental tissue. A protocol can help dentists, especially by describing the stages of making direct no-prep veneers, achieving better clinical success. This article presents a clinical step-by-step guide approach to fabricating light-cured composite prepless veneers, using opaque and tints to mask dark backgrounds, and preserving the dental substrate in a patient with multiple restorations, changes in color, and shape. Light-cured composite prepless veneers comply with the principles of biomimetics and offer advantages such as conservation of dental structures, versatility, one-session preparation, cost, mechanical properties, and excellent aesthetics, however, skill with this technique is required.

Automatic Final-Product Assessment of Virtual Reality Mastoidectomy Performance: A Validity and Reliability Study.

Assessment is key in modern surgical education to monitor progress and document sufficient skills. Virtual reality (VR) temporal bone simulators allow automated tracking of basic metrics such as time, volume removed, and collisions. However, adequate performance assessment further includes compound rating of the stepwise bony excavation, and exposure and preservation of soft tissue structures. Such complex assessment requires further development of automated assessment routines in the VR simulation environment. In this study, we present the integration of automated mastoidectomy final-product assessment with validation against manual rating. At two international temporal bone courses, 33 ORL trainees performed anatomical mastoidectomies in the Visible Ear (VR) Simulator with automatic performance assessment using a newly implemented rating routine based on the modified Welling Scale. Automated assessment was compared with manual ratings by experts using absolute agreement, intraclass correlation, and generalizability analysis to establish validity and reliability. The overall average agreement between manual and automatic assessment was 83.9% compared with the inter-rater agreement of 88.9%. A majority of items (15 out of 26) showed high agreement between automated and manual rating (>85%). Intraclass correlation coefficients were found to be high. Generalizability analysis with D-studies found that five repetitions per participant are needed for a G coefficient >0.8, which is considered necessary for high-stakes assessments. We have demonstrated the feasibility, validity, and reliability of an automatic assessment system integrated into a VR temporal bone simulator. This can prove to be an important tool for future self-directed training with skills certification.

Neuroprotective potential of Alpinia calcarata and HPLC analysis of stigmasterol against cisplatin-induced neuropathy in rat model

Cisplatin is a chemotherapeutic agent known for causing severe peripheral neuropathy as a side effect, impacting patients’ quality of life by damaging nerve tissues. This study aims to explore the neuroprotective effects of the ethanolic extract of Alpinia calcarata Roscoe rhizome (EEACR) and stigmasterol identified by high-performance liquid chromatography (HPLC) in a rat model of cisplatin-induced neuropathy. Male Wistar rats were divided into control, cisplatin-induced neuropathic, and two intervention groups receiving different concentrations of EEACR (250 and 500 mg/kg). Neuropathy was induced with cisplatin administered intraperitoneally at a dose of 2 mg/kg per week for five weeks. The intervention groups were treated orally with EEACR daily during the induction period. Treatment with EEACR showed a significant attenuation of neurotoxicity as evidenced by behavioural improvements in mechanical allodynia, thermal hyperalgesia, and cold allodynia. HPLC analysis confirmed the presence of stigmasterol in EEACR, which may contribute to its therapeutic effects. Biochemical assessments revealed a significant decrease in oxidative stress markers and an increase in antioxidant enzyme activities, including superoxide dismutase, catalase, and glutathione peroxidase, in the nervous tissues of EEACR-treated rats. Histopathological examination indicated a reduction in nerve damage and enhanced preservation of myelin and axonal structures in the treatment groups. The findings from this study suggest that EEACR, enriched with stigmasterol, offers promising neuroprotective effects against cisplatin-induced neuropathy in rats.

Improving the radiological prediction of surgical resection of nonfunctioning pituitary adenomas.

Nonfunctioning pituitary adenomas (NFPAs) are benign tumors growing in the sellar region. Total surgical excision of the lesion is recommended as the preferred treatment choice with preservation of adjacent structures. The objective is to establish a radiological score to predict the feasibility of NFPA total surgical excision. Patients with treatment-naïve NFPA who underwent a transsphenoidal approach and sellar magnetic resonance imaging (MRI) in the preoperative period and 3 months after surgery were included. Data on age, sex, tumor diameter, extrasellar extension, postoperative cure rates, and hormone and transcription factor expression were collected. A combined score was proposed based on Knosp and SIPAP classifications. We proposed 3 classification groups depending on the tumoral extension to the suprasellar, infrasellar, anterior and posterior directions of the sellar region. A total of 164 patients were included in the study, and 85 (52%) were female. Total excision was obtained in 46% (n = 75) of the patients. The majority of tumors were of gonadotrophic lineage (59%), followed by corticotrophic (17%) and other less common types. Largest tumor diameter was 6.8cm [mean 3.8cm (± 1.1cm)]. From the established groups, 10 patients were classified in Group I, of whom 8 (80%) patients underwent total excision, 115 patients were classified in Group II, of whom 58 (50%) underwent complete excision and 39 patients in Group III, of whom 9 (23%) underwent complete excision (p value < 0.001). The newly proposed score helps to determine the feasibility of total NFPA excision, allowing for better surgical planning and predictions of postoperative outcomes.

Soft sensor model for endpoint carbon content and temperature in BOF steelmaking based on just-in-time learning with multilayer structure preservation and sparse information enhancement

Ensuring precise prediction of the endpoint carbon content and temperature is paramount in controlling the endpoint of the basic oxygen furnace (BOF) steelmaking process. However, due to the frequent fluctuations in real-time blowing conditions, the conventional just-in-time learning soft sensor approach encounters challenges in effectively anticipating the blowing endpoint. To address these aforementioned issues, this research introduces a novel approach known as the pattern-oriented multilayer structure preservation and sparse information enhancement model (POMLSP-SIE). This approach involves dynamically generating a dataset with the same distribution based on the characteristics of the query sample pattern. It is combined with a multi-pathway dimension reduction model to preserve multi-view spatial geometry information while reducing data dimension. The low-dimensional embedding serves as dictionaries containing various hierarchical structural characteristics. Significant information within these dictionaries is sparsely represented to amplify its influence during the regression prediction process while diminishing the impact of less relevant information. This refinement aims to rectify the problem of static regression models being weak to adapt to changing working conditions, ultimately enhancing prediction performance. The effectiveness of the proposed method is substantiated through an experimental study utilising real converter steelmaking process data, thereby confirming its practical applicability.

Enhancement of luminescence and thermal stability in Eu3+-doped K3Y(BO2)6 with Li+ and Na+ co-doping

Eu3+-doped and Li+/Na+ co-doped K3Y(BO2)6 (KYBO) phosphors were synthesized through a microwave-assisted sol–gel method, and their structural and photoluminescent (PL) characteristics were examined. X-ray diffraction (XRD) and Rietveld refinement confirm effective dopant incorporation and preservation of the crystalline structure. Fourier Transform Infrared (FTIR) spectroscopy indicates the maintenance of the borate structure, confirming the structural integrity of the phosphors upon doping. The addition of Li+ and Na+ co-dopants notably enhances luminescent efficiency and thermal stability, making these phosphors promising candidates for solid-state lighting (SSL) applications. PL analysis reveals strong red emission peaks at 612 nm, attributed to the 5Do → 7F2 transition of Eu3+ ions. The study indicates that electric dipole-quadrupole interactions are the primary mechanism for energy migration, with a critical distance of approximately 22.68 Å. This mechanism contributes to concentration quenching at higher doping levels. High temperature PL measurements indicated an activation energy of 0.1389 eV for thermal quenching in the Li+ co-doped sample. Additionally, the Na+ co-doped sample exhibited an abnormal thermal stability behavior, with an even higher activation energy of 0.2536 eV. This suggests that Na+ co-doping significantly enhances the thermal resilience of the phosphor, making it more suitable for high-power light-emitting applications that operate under extreme conditions. CIE chromaticity diagrams highlight the potential for optimizing Eu3+ doping levels, combined with Li+ and Na+ co-doping, to improve luminescent performance and thermal stability for advanced SSL applications.

Novel amino-functionalized MOF-based sensor for zinc ion detection in water and blood serum samples

Aquatic systems with low zinc levels can experience a significant decrease in carbon dioxide uptake and limited growth of phytoplankton species. In this study, we describe the use of a new fluorescent sensor based on NH2-MIL-53(Al), and modified with glutaraldehyde and sulfadoxine, for selectively detecting zinc ions in water and blood serum samples. Characterization of the synthesized material was performed using X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), Brunauer–Emmett–Teller (BET) surface area analysis, X-ray photoelectron spectroscopy (XPS), and scanning electron microscopy (SEM), confirming successful functionalization and preservation of the MOF structure. The sensor’s performance for Zn2+ detection was evaluated by spectrofluorometry, demonstrating a significant fluorescence enhancement upon Zn2+ binding due to the interaction between Zn2+ ions and the sulfonamide groups. With a detection limit as low as 3.14 × 10–2 ppm, the sensor demonstrates high selectivity for Zn2+ over other common metal ions. The sensor’s response is rapid, stable, and reproducible, making it suitable for practical applications. Real sample analysis was conducted in tap water and blood serum samples, with the results compared to those obtained using ICP-OES and a colorimetric test with 5-bromo-PAPS. The comparison confirmed the high accuracy and reliability of the fluorescent sensor in detecting Zn2+ ions in complex matrices. NH2-MIL-53(Al) modified with glutaraldehyde and sulfadoxine shows potential as a selective fluorescent sensor for Zn2+ detection, making it a valuable tool for monitoring the environment and biology.

Advancing bamboo fiber reinforcement: A novel approach using eco-friendly plant ash alkali treatment.

This study aimed to enhance the engineering performance of bamboo fibers for use in composite materials by employing a sustainable plant ash alkali treatment. The primary objective was to improve the tensile strength, crystallinity, and thermal stability of bamboo fibers, which are known for their high specific strength, low density, and biodegradability but suffer from poor compatibility with hydrophobic matrices. The study involved treating bamboo fibers with varying concentrations of plant ash solutions (5 %, 10 %, 20 %, and 30 % by mass fraction) and comparing the results with those from conventional NaOH treatment. The results showed that plant ash treatment at a 20 % concentration significantly improved tensile strength by 14.16 % compared to untreated fibers, increased the crystallinity index to 75.57 %, and enhanced thermal stability, retaining more residual mass at high temperatures than NaOH-treated fibers. These improvements are attributed to the reduction in fiber polarity and better preservation of fiber structure. The findings suggest that plant ash, as a cost-effective and eco-friendly alternative to NaOH, can significantly enhance the mechanical and thermal properties of bamboo fibers, making them suitable for use in environmentally sustainable composite materials in construction, and other industries.

Establishing Benchmarks for Airway Replacement: Long-Term Outcomes of Tracheal Autografts in a Large Animal Model.

Airway replacement is a challenging surgical intervention and remains an unmet clinical need. Due to the risk of airway stenosis, anastomotic separation, poor vascularization, and necrosis, it is necessary to establish the gold-standard outcomes of tracheal replacement. In this study, we use a large animal autograft model to assess long-term outcomes following tracheal replacement. Four New Zealand White rabbits underwent tracheal autograft surgery and were observed for 6 months. Clinical and radiographic surveillance were recorded, and grafts were analyzed histologically and radiographically at endpoint. All animals survived to the endpoint with minimal respiratory symptoms and normal growth rates. No complications were observed. Computed tomography scans of the post-surgical airway demonstrated graft patency at all time points. Histological sections showed no sign of stenosis or necrosis with preservation of the native structure of the trachea. We established benchmarks for airway replacement. Our findings suggest that a rabbit model of tracheal autograft with direct reimplantation is feasible and does not result in graft stenosis or airway collapse.

On Lie Homomorphisms of Complex IntuitionisticFuzzy Lie Algebras

In this paper, we investigate the properties of complex intuitionistic fuzzy Lie subalgebras and ideals under Lie algebra homomorphisms, focusing on both images and inverse images. We provide detailed proofs for several new results concerning the preservation of complex intuitionistic fuzzy structures through homomorphisms, and we introduce additional homomorphism-related properties for these structures. This work extends known results to the context of complex intuitionistic fuzzy Lie algebras, contributing new insights into their behavior under algebraic mappings.

Cryo-X-Ray Phase Contrast Imaging Enables Combined 3D Structural Quantification and Nucleic Acid Analysis of Myocardial Biopsies.

Snap-frozen biopsies serve as a valuable clinical resource of archival material for disease research, as they enable a comprehensive array of downstream analyses to be performed, including extraction and sequencing of nucleic acids. Obtaining three-dimensional (3D) structural information before multi-omics is more challenging but can potentially allow for better characterization of tissues and targeting of clinically relevant cells. Conventional histological techniques are limited in this regard due to their destructive nature and the reconstruction artifacts produced by sectioning, dehydration, and chemical processing. These limitations are particularly notable in soft tissues such as the heart. In this study, the feasibility of using synchrotron-based cryo-X-ray phase contrast imaging (cryo-X-PCI) of snap-frozen myocardial biopsies is assessed and 3D structure tensor analysis of aggregated myocytes, followed by nucleic acid (DNA and RNA) extraction and analysis. It is shown that optimal sample preparation is the key driver for successful structural and nucleic acid preservation which is unaffected by the process of cryo-X-PCI. It is proposed that cryo-X-PCI has clinical value for 3D tissue analysis of cardiac and potentially non-cardiac soft tissue biopsies before nucleic acid investigation.

Alda-1 Ameliorates Oxidative Stress-Induced Cardiomyocyte Damage by Inhibiting the Mitochondrial ROS/TXNIP/NLRP3 Pathway.

Alda-1 functions as an agonist of aldehyde dehydrogenase (ALDH2) within the mitochondria, contributing to the preservation of mitochondrial structure and function. This study aimed to determine whether Alda-1 inhibited the accumulation of mitochondrial reactive oxygen species (mtROS) and improved cardiomyocyte damage under oxidative stress. Cardiomyocytes derived from human induced pluripotent embryonic stem cells (iPSC-CMs) and human AC16 cardiomyocytes were chosen for the experiments. Oxidative stress was induced in both cardiomyocyte types using hydrogen peroxide (H2O2), a commonly employed agent. The mtROS accumulation and mitochondrial functional status were assessed by measuring the ROS content, mitochondrial membrane potential, and mitochondrial respiratory chain function. Co-IP experiments were used to analyze whether mtROS promoted protein interactions between TXNIP and NLRP3 and induced NLRP3 inflammasome activation. The results showed that Alda-1 mitigated damage to mitochondrial structure and function under oxidative stress, concurrently reducing the accumulation of mtROS. Co-IP experiments revealed that elevated mtROS levels attenuated the protein interaction between TXNIP and TRX while intensifying the interaction between TXNIP and NLRP3. Consequently, this triggers activation of the NLRP3 inflammasome, leading to cardiomyocyte damage. In contrast, TXNIP knockdown inhibited H2O2-induced myocardial injury and enhanced the therapeutic effects of Alda-1. Collectively, the results show that, in an H2O2 environment, Alda-1 increased the production of ALDH2 activity in cardiomyocytes, hindered the production of mtROS, disrupted the interaction between TXNIP and NLRP3, and alleviated myocardial damage during oxidative stress.

Cardiomyocyte regeneration in human myocarditis

Abstract Background Newly generated cardiomyocytes (NGC) concur to recovery of human myocarditis. Purpose describe NGC’s contribute with age and their modality of myocardial homing and integration. Methods We retrospectively assessed NGC in 213 consecutive patients with endomyocardial biopsy diagnostic for myocarditis, normal coronaries and valves. Tissue samples were processed for histology and immune-histochemistry for CD3+ T lymphocytes, alpha-sarcomeric-actin, junctional connexin-43, Ki-67, phosphorylated STAT3 (p-STAT3) and Western Blot (WB) for HMGB1. Frozen samples were analyzed with polymerase chain reaction (PCR) for cardiotropic viruses. Controls included 20 normal surgical biopsies. NGC were defined as small myocytes (diameter &amp;lt; 10 µ) with nuclear positivity to Ki-67 and p-STAT3, positive immunostaining for cytoplasmic alpha-sarcomeric-actin and connexin 43. Their number/mm2, relation with age and pathway of integration were evaluated. Results PCR was positive for viral genomes in 16% of cases. NGC mean diameter was 6.6 ± 3.34 vs 22.5 ± 3.11 µm adult cells; their number, in comparable LVEF, was 86.3 ± 10.3/mm2 in patients between 10 and 40 years; 50.4 ±13.8/mm2 in those between 41 and 60; 15.1 ± 5.7/ mm2 in those between 61 and 80; control NGC were 0.2± 0.2/mm2 ; NGC were no statistically different in viral and non-viral myocarditis. HMGB1 and STAT-3 were remarkably higher than controls. NGC crossed the membrane and grew integrated within the empty necrotic myocytes. Conclusions NGCs are constantly recognizable in acute human myocarditis. Their number declines with age. Their integration within necrotic myocytes consents preservation of cardiac structure and function.

Comparative Study on Structural Differences in Monosaccharide Layers Using PLD and PED Techniques.

To demonstrate the feasibility of obtaining low-molecular-weight organic films (below 200 Da) using non-solvent PVD processes, glucose layers were produced via pulsed laser deposition (PLD) and pulsed electron beam deposition (PED) methods. Glucose was chosen due to its fundamental role in various biological processes, and because this low-molecular-weight compound is a solid at room temperature, which is required for both techniques. The physical and chemical structures of the deposited glucose layers were characterized by optical, scanning electron, and atomic force microscopy, as well as by X-ray diffraction, X-ray photoelectron, and infrared spectroscopy. Both PLD and PED methods resulted in glucose layers with good chemical structure preservation (with minor oxidation observed in PED) while yielding films with distinct physical properties. This opens up the possibility of tailoring organic layers with specific characteristics depending on the application, by choosing the deposition method.