A study of recently acquired material of Herniosina Roháček, 1983 (Diptera: Sphaeroceridae: Limosininae) in the Mediterranean subregion revealed a new species, H.calabrasp. nov. (Italy: Calabria: Serre Calabresi Mts) and the first females of H.erymantha Roháček, 2016 (Greece: southern Peloponnese: Taygetos Mts). Herniosinacalabrasp. nov. (both sexes) and the female of H.erymantha are described and illustrated in detail including structures of terminalia, their relationships are discussed and new information on their biology (habitat association) is given. An update of a key to all know species of Herniosina species is presented.