Structure Of System Research Articles

Deriving the PedsUtil health state classification system to measure health utilities for pediatric populations based on the PedsQL: a confirmatory factor analysis.

An important methodological challenge in conducting pediatric economic evaluations is estimating the preference-based health-related quality of life (HRQoL) of children. Current methods are highly variable and there is no single instrument available to value HRQoL consistently across multiple pediatric age groups. The Pediatric Quality of Life Inventory (PedsQL) is a non-preference-based generic HRQoL instrument validated for children 2-18 years, but it cannot be directly used in economic evaluations. The aim of this study was to establish the core dimension structure of the PedsUtil health state classification system using confirmatory factor analysis, which is the first step of deriving a preference-based measure of HRQoL based on the PedsQL. Four competing dimension structures of the PedsUtil health state classification system were developed based on published literature and expert opinion. Using data from the Longitudinal Study of Australian Children (LSAC) (n = 45,207), the 4 dimension structures were evaluated using the robust weighted least squares estimation method. The analyses were stratified by 2-year age intervals (from 2 to 17 years) to reflect the study design of the LSAC, as well as special healthcare needs status of the child. Model fit was evaluated by examining standardized factor loadings and various fit indices including the comparative fit index (CFI), Tucker-Lewis Index (TLI), and the root mean square error of approximation (RMSEA). Modification indices and residual correlations were examined to re-specify the models to improve model fit when necessary. The findings supported a 7-dimension structure (i.e., Physical Functioning, Pain, Fatigue, Emotional Functioning, Social Functioning, School Functioning, and School Absence) of the PedsUtil health state classification system. The 7-dimension model exhibited adequate fit across subgroups with CFI values that ranged from 0.929 to 0.954, TLI values from 0.916 to 0.946, and RMSEA values from 0.058 to 0.102. This study established the core dimension structure of the PedsUtil health state classification system using confirmatory factor analysis. The 7-dimension structure was found to be applicable across diverse pediatric populations. Research is currently ongoing to select the most representative item within each dimension of the PedsUtil health state classification system and valuation surveys will be fielded to estimate the PedsUtil scoring system.

Male reproductive system of the deep-sea acorn worm Quatuoralisia malakhovi (Hemichordata, Enteropneusta, Torquaratoridae) from the Bering Sea

BackgroundThe deep-sea acorn worm Quatuoralisia malakhovi belongs to the phylum Hemichordata, class Enteropneusta, family Torquaratoridae, which was described in 2005. Owing to their epibenthic lifestyle and deep-sea habitat features, torquaratorids differ anatomically from shallow-water acorn worms; however, their morphology and fine structure are poorly studied. We have the opportunity to make three complete detailed series of histological sections of Q. malakhovi and to study the microscopic anatomy, histology and fine structure of the reproductive system of this acorn worm using scanning and transmission electron microscopy.ResultsThe sexes of Q. malakhovi are separate and indistinguishable externally. The lobed testes occupy the dorsal side of the genital wings and distinctly bulge into the peribranchial cavity by their mature lobes. The central part of the testis is always submerged into the genital wing and opens via a single gonad pore. The monociliary muscle cells stretch along the external wall of the testis and surround the gonad pore, probably taking part in the contraction of the testis lobes for spawning. The germinative epithelium of the testis contains spermatogenic cells at different stages of development and interstitial cells. Yolk cells are not found. Interstitial cells embrace the spermatogonia and spermatogenic columns, providing horizontal compartmentalization of the germinative epithelium, and contain numerous phagosomes with remnants of degenerating spermatogenic cells. The testis wall contains haemal lacunae, which are usually located on the side opposite the gonad pore. We describe the fine structure of spermatogonia, spermatocytes clustered in spermatogenic columns, spermatids, and spermatozoa. Spermatozoa are of the ectaquasperm type and consist of an acorn-shaped head and a flagellum 18–25 µm long. The sperm head includes a beak-shaped acrosomal part, a spherical nucleus and a midpiece containing a ring of 5 or rarely 6 mitochondria.ConclusionsThe male reproductive system and sperm structure of Q. malakhovi, a representative of the family Torquaratoridae, have a number of differences from shallow-water acorn worms; however, the spermatogenesis and sperm structure of Q. malakhovi generally follow the pattern of the other three enteropneust families, and the phylogenetic significance of these deviations should be the subject of further research.

Myelodysplasia Rehabilitation in Children: a Review

INTRODUCTION. 1.5 thousand children with myelodysplasia (MD) are born in Russia every year. To improve the quality of life, most of them need lifelong medical supervision and rehabilitation. The topic of medical rehabilitation in MD in children is poorly covered. AIM. To search and analysis published data on the rehabilitation of children with MD identify the most effective methods. MATERIALS AND METHODS. The collection and analysis of publications on this topic from the PubMed and eLibrary databases published from 2014 to 2024 was carried out. Earlier fundamental works on secondary disorders in myelodysplasia and Spina bifida were also included in the review. A total of 512 publications in PubMed and 1096 publications in eLibrary were analyzed, 584 of them were unique. Publications of reviews, systematic reviews and clinical observations were included. RESULTS AND DISCUSSION. Rehabilitation for MD in children is multidisciplinary in nature, a violation of the structure of the nervous system entails, already in utero, the formation of deformities of the musculoskeletal system, combined changes in the functioning of the lower urinary tract and intestines. It is known that rehabilitation started from an early age helps to master motor skills in a timely manner, contributes to the prevention of metabolic syndrome, contractures, pressure sores, urodynamic disorders and constipation. There are different approaches regarding the intensity of physical activity during the day and week, following the recommendations of the World Health Organization (WHO), children should exercise for more than 60 minutes a day. Physical exercises should be carried out from the perspective of the concept of neuroplasticity and enhanced by the influence of magnetic stimulation, low-intensity laser, electromyostimulation, vibration therapy. It will be possible to effectively use acupuncture and massage. In addition, it is important to include urological and orthopedic care in medical rehabilitation from an early age. CONCLUSION. Due to the high disability, there is a question of effective methods for improving rehabilitation potential and improving the standard of living. Among the described methods, physiotherapy is distinguished, including therapeutic exercises, magnetic stimulation, low-intensity laser therapy, electromyostimulation, and ergotherapy. It is necessary to continue to study the effectiveness of the use of various physical factors in children with MD.

Design of geostationary orbit ultrawide FOV long-wave infrared imaging spectrometer

The geostationary orbit imaging spectrometer offers distinct advantages for diverse applications in remote sensing. To enhance swath and data richness, there is a growing trend toward spectrometers with broader fields of view (FOV) and extended slit lengths. Optical systems equipped with these features face significant challenges in aberration correction. This paper investigates the parameters of geostationary orbits and designs a spectroscopic system capable of achieving ultrawide FOV without the need for stitching spectrograph subsystems. Additionally, it also designs a front-end telescope system that matches the spectroscopic system. The spectroscopic system features a slit length of 240 mm, employing an enhanced Offner structure for a long slit spectroscopic system, incorporating a freeform surface on the third mirror. To inhibit the effects of radiation stray light, the system's exit pupil is controlled to be positioned in front of the image plane to facilitate matching with the cold shield. The analysis indicates that the spectral resolution of the long-wave infrared imaging spectrometer system is 50 nm, with a total of 90 spectral sampling channels. The system's spatial resolution is 100 m, enabling a swath width of 400 km. Furthermore, the modulation transfer function (MTF) exceeds 0.42 at the Nyquist frequency of 8.35 lp/mm. The maximum RMS radius of the system is less than 27 μm at wavelengths ranging from 8 to 12.5 μm.

Abdominal aortic aneurysm repair in Sweden vs. Finland: benefits of population-wide screening.

In 2006, screening of 65-year-old men for abdominal aortic aneurysm (AAA) was started in Sweden. Decline in aneurysm-related mortality has been reported since, but aneurysm incidence has been diminishing globally. Neighbouring Finland with similar population structure and health care system has no AAA screening programme. The aim of this study was to compare incidence and results of AAA repair in Sweden and Finland to differentiate the effect of screening from other changes in the epidemiology and treatment of AAA. All repairs for intact AAA (iAAA) or ruptured AAA (rAAA) from 1998 to 2017 were identified from national registers, and mortality data for these patients were collected. A total of 15 927 operations for iAAA were performed in Sweden and 6933 in Finland. In Sweden, the yearly operation volume increased after introduction of screening. Both countries showed a decrease in number of rAAA operations, but the decrease was more pronounced in Sweden. Sweden had a higher proportion of all AAA repairs because of rupture in the start of the study but by the end, the proportions were similar in both countries. Long-term survival improved for 65-79-old men in Sweden after start of screening. This study reveals improvements in results of AAA repair in Sweden. A decrease in rAAA repair and increase in iAAA repair were evident after AAA screening was started in 2006 and resulted in better outcomes. These changes are likely the result of AAA screening as they cannot be seen in neighbouring Finland that is lacking an AAA screening programme.

Passive control and damage assessment with a pendulum tuned mass damper using mean square equations and second-order eigen-perturbation techniques

This paper proposes a novel framework for damage detection and retuning of a Pendulum Tuned Mass Damper (PTMD) addressing effects of stochasticity via mean-square equations and establishing rapid change detection. The framework was made possible via a Stochastic Differential Equation (SDE) formulation of the equations of motion considering a two degree of freedom base and structure system equipped with a PTMD. The system was assumed to be excited at the base with Gaussian white noise and the solutions were computed using a stochastic integration scheme. A Second Order Eigen-Perturbation (SOPS) estimate is used to carry out detection of sudden stiffness reduction in the base floor of the structure. Once damage is detected, mean square equations on monitored displacements were used to retune the PTMD by adapting the length of the pendulum. It was found that damage could be detected rapidly and successive retunings were able to minimize the mean square displacement of the damaged structure and stabilise it over time.

Reliability optimization of reliability-redundancy allocation problems based on K-mixed strategy

In a reliability-redundancy allocation problem (RRAP), system reliability is maximized by selecting component reliabilities and finding the most suitable redundancy of subsystems. The advantages of a K-mixed strategy were introduced by modeling, so as to better improve system reliability. Compared with other existing redundancy strategies, the performance of a K-mixed strategy was verified using redundancy allocation problems (RAP). In this study, for the first time, a reliability calculation model under the new structure ( [Formula: see text] = 2 and [Formula: see text] = 1) is proposed, and the K-mixed strategy under the new structure is used in the reliability-RAP (RRAP), which is more complex than the RAP and further saves the production cost. In practical optimization, there was a complex decision-making problem to ensure optimal system reliability while minimizing system volume, weight, and cost. Then, this K-mixed strategy was adopted for modeling three benchmark problems in RRAP to seek a better and more flexible system structure. A powerful evolutionary algorithm (NSGA-II) was used to solve the new RRAP model to obtain the best system structure and reliability. The advantages of this model were confirmed by comparison with results from previous reliability optimization studies. The results show that the cost-saving advantages of the new structure in ensuring maximum reliability are significant. All the optimized remaining costs are noticeably higher than those of other methods, with the cost savings of the series-parallel system being the greatest. The difference in remaining costs compared to previous optimizations remains in the tens. Moreover, in more complex systems (Complex bridge system), the advantage in remaining volume is very significant, with the improvement being three times that of the optimization results of other methods.

Electrical stimulation improves the efficiency of treating typical reclaimed water in biofilters

ABSTRACT To conduct advanced treatment on reclaimed water with a low C/N ratio and a low content of biodegradable organic matter, this paper established an electrically coupled biological filter system (biofilter-microbial electrolysis cell, BF-MEC). Through two-stage influent, the influence of electrical stimulation and electrochemical structure on the nitrogen and carbon removal of the system was explored, and the following results were obtained. The optimal externally applied voltage for the BF-MEC system is 0.55 V. The optimal structure of the BF-MEC system is an electrode spacing of 100 mm, and the optimal electrode position is when the electrode is located at the bottom of the system. Compared with the BF system, in the first stage, the removal rates of chemical oxygen demand, total ammonia nitrogen (TAN), and total nitrogen (TN) in the optimal structure BF-MEC system are increased by 13.4, 118.1, and 273.65%, respectively. In the second stage, COD, TAN, and TN removal rates are increased by up to 277.98, 115, and 722.98, respectively. The optimal electrode position is when the electrode is located at the lower part of the system. At this time, the microbial community is more abundant, the microbial activity and lifespan are longer, and the system efficiency is optimal. optimal.

Fundamental study on the difference between materials and vibration conditions in piezoelectric vibration power generation using electromechanical coupling phenomena

Conventionally, vibration energy and sound energy are emitted as noise and vibration. We have proposed a system that converts the vibration of the plate into energy. In this study, a circular plate of constant thickness, on which a piezoelectric element was installed, was adopted as the host structure of an energy-harvesting system. The plate was supported at its elastic end, which was intermediate between simple and clamped supports, and was subjected to a harmonic point force. The piezoelectric performance of the system was highly dependent on the vibration characteristics of the plate. Regarded as one of the representative vibrational characteristics, the natural frequency of the plate was affected by the materials, excitation positions, and vibration modes of the plate. Therefore, the plate materials varied the natural frequency, thus affecting the conversion of vibration energy into electrical energy through the piezoelectric effect. In this study, we investigated plates made of selected materials commonly used in industry and tested different excitation conditions. As the result, we clarified that the proposed system is almost unaffected by the material of the plates, and it was shown to be useful.

Development of an expert system for technological support of required roughness when machining hardened steel parts on numerically controlled machines

This work addresses the problem of improving the surface quality of parts of rotation bodies, which are made of hardened steels, in the course of their machining on lathes with numerical program control. The research object was the surface roughness of parts. The methodology involved system analysis and synthesis, artificial neural networks, fuzzy logic, experiment, and processing of experimental results. A decomposition scheme for the expert system structure was developed, which can be used as the basis for formulating requirements to a future expert system for monitoring and prediction of roughness parameters. It was established that the models currently applied to describe the relationship between surface quality and the technological regimes used to ensure the technological level of roughness give a high error of over 20%. The possibility of using models that apply artificial intelligence and contain neural network blocks and decision-making devices based on fuzzy logic is substantiated. It is shown that such a combination makes it possible to customize the system for processing parts of a certain production range, as well as a more correct assessment of the onset of catastrophic wear of cutting tools. The neuro-fuzzy model was confirmed to have an error of less than 10%, which is significantly lower than when using spectral or correlation models. According to the testing results, the proposed expert system for monitoring and prediction of roughness parameters enables a 2.5-fold reduction in the scatter of roughness parameters under an increase in tool wear, compared to without its application. Thus, the proposed system makes it possible to assess the level of cutting tool wear more correctly and determine the onset of its limit state.

Improving the organization of medical care for patients in the specialty of medical rehabilitation in the Tomsk oblast

More than a third of the total population of the planet needs medical rehabilitation and the need will increase in the future. The prospective increase in life expectancy by the middle of the 21st century will be accompanied by an increase in the share of malignant neoplasms and diseases of the circulatory system in the morbidity structure, and the level of injuries will remain unchanged. These factors will negatively affect a person’s functional capabilities and will contribute to an increase in disability, increasing the relevance of medical rehabilitation. In Russia, according to the current procedure, there is a three-stage organization of medical rehabilitation. The implementation of these stages is carried out within the regional territorial programs of state guarantees (TPSG) of free medical care to citizens, which limits the financial accessibility of the completed cycle of medical rehabilitation for patients, due to budgetary allocations and the compulsory health insurance program, negatively affecting the quality of care provided. The article assesses the volume of medical rehabilitation provided in inpatient settings in the TPSG of the Tomsk oblast for the period of 2019–2022. Currently, three medical organizations in the region of different forms of ownership provide medical rehabilitation. In total, over a four-year-period, 8,804 cases in the specialty of medical rehabilitation were completed in the amount of 378.9 million rubles. An underfulfillment of the volume and costs of medical care during medical rehabilitation approved by the TPSG was recognized, as well as an outflow of patients for treatment to other regions (1,864 people during the study period). The most popular clinical and statistical groups (CSG) for which residents of the Tomsk oblast need rehabilitation measures have been identified, and organizational ways to improve the profile of this medical care have been substantiated.

Engineering Memory T Cells as a platform for Long-Term Enzyme Replacement Therapy in Lysosomal Storage Disorders

Enzymopathy disorders are the result of missing or defective enzymes. Amongst these enzymopathies, mucopolysaccharidosis type I, is a rare genetic lysosomal storage disorder caused by mutations in the gene encoding alpha-L-iduronidase (IDUA), ultimately causes toxic build-up of glycosaminoglycans (GAGs). There is currently no cure and standard treatments provide insufficient relief to the skeletal structure and central nervous system (CNS). Human memory T cells (Tm) migrate throughout the body’s tissues and can persist for years, making them an attractive approach for cellular-based, systemic enzyme replacement therapy. Here, we tested genetically engineered, IDUA-expressing Tm as a cellular therapy in an immunodeficient mouse model of MPS I. Our results demonstrate that a single dose of engineered Tm leads to detectable IDUA enzyme levels in the blood for up to 22 weeks and reduced urinary GAG excretion. Furthermore, engineered Tm take up residence in nearly all tested tissues, producing IDUA and leading to metabolic correction of GAG levels in the heart, lung, liver, spleen, kidney, bone marrow, and the CNS, though only minimal improved cognition was observed. Our study indicates that genetically engineered Tm holds great promise as a platform for cellular-based enzyme replacement therapy for the treatment of mucopolysaccharidosis type I and potentially many other enzymopathies and protein deficiencies.

Uncertainty and Latin Hypercube Sampling in Geotechnical Earthquake Engineering

A soil–foundation–structure system (SFSS) often exhibits different responses compared to a fixed-base structure when subjected to earthquake ground motion. Both kinematic and inertial soil–foundation–structure interactions can significantly influence the structural performance of buildings. Numerous parameters within an SFSS affect its overall response, introducing inherent uncertainty into the solution. Performing time history analyses, even for a linear elastic coupled SFSS, requires considerable computational effort. To reduce the computational cost without compromising accuracy, the use of the Latin Hypercube Sampling (LHS) technique is proposed herein. Sampling techniques are rarely employed in soil–foundation–structure interaction analyses, yet they are highly beneficial. These methodologies allow analyses determined by sampling to be conducted using commercial codes designed for deterministic analyses, without requiring any modifications. The advantage is that the number of analyses determined by the sampling size is significantly reduced as compared to considering all combinations of input parameters. After identifying the important samples, one can evaluate the seismic demand of selected soil–foundation–bridge pier systems using finite element numerical software. This paper indicates that LHS reduces computational effort by 60%, whereas structural response components (translation, rocking) show distinct trends for different systems.

An investigation on main effects and interactions of relative age effects and playing position in female elite football in Germany

Athlete age-grouping and age-banding has been shown to impact sport participation and athlete development. The current study examined the impact of within-year (WYEs) and between-year effects (BYEs), and their interactions with playing position, on female participation in elite German football. The sample of 1,378 German first-league players revealed expected participation inequities within-year with relatively older players being over-represented and younger players being under-represented. From a between-year perspective there were no participation differences. The interaction of WYEs and BYEs revealed an over-representation of odd-year players in Q1, and Q2 in even years. With respect to the interaction between year effects and playing position, for WYEs the expected effect was present for goalkeepers and defenders, while there was no significant interaction for BYEs. Overall, the results offer insight regarding the impact of system structure on participation, and highlight unique characteristics associated with playing positions.

A Trachytic Monogenetic Volcano in the Changbaishan Volcanic Field, Xitudingzi Volcano: Petrogenesis, Magma Plumbing System and Implications for Non-basaltic Monogenetic Volcanism

Abstract The Changbaishan volcanic field is the largest Quaternary volcanic field in China and includes the most dangerous polygenetic volcano, Tianchi volcano. In addition to the Tianchi volcano, the Changbaishan volcanic field contains two other polygenetic volcanoes (Wangtian’e volcano and Namphothe volcano) and at least 150 monogenetic volcanoes. The compositions of these monogenetic volcanoes range from basalt to rhyolite. Basaltic monogenetic volcanoes are the most abundant and spread throughout the volcanic field, whereas intermediate to evolved monogenetic volcanoes are found only within the Touxi subfield. We know very little about these non-basaltic monogenetic volcanoes, especially the magmatic petrogenesis, volcanological origins and structure of the magma plumbing system beneath them. Therefore, in this study, the Xitudingzi volcano within the Touxi subfield is used as an example to study the petrogenesis of silicic magma and explore the structure of the magma system by combining new 40Ar/39Ar dating, whole-rock geochemistry, Sr-Nd isotopes, and mineral chemistry. The 40Ar/39Ar chronology results showed that the Xitudingzi volcano erupted at 1.17 Ma; this volcano is younger than the other monogenetic volcanoes in the Touxi subfield. The geochemical results indicated that the eruption products of the Xitudingzi volcano are trachytic and formed from the assimilation fractional crystallization (AFC) process of an alkaline basaltic parental magma. The results from the Rhyolite-MELTS and thermobarometry indicated that the magma plumbing system of the Xitudingzi volcano contained two magma chambers at depths of 8 km and 19 km, respectively. The trachytic magma of Xitudingzi was produced by the AFC process from the alkaline basaltic magmas in the deep magma chamber and then transported to the shallow magma chamber. Furthermore, differences in the trace element concentrations between the different units of the Xitudingzi volcano indicated that the shallow pre-eruptive magma chamber was compositionally zoned. Based on the results from our study, the upper crustal magma plumbing system of the Xitutingzi volcano existed independently of the magma plumbing system of the cone-construction stage of the Tianchi volcano, even though the two volcanoes had similar ages of activity as well as the same chemical composition of eruptive products. Based on these results and other existing studies, a relatively thick crust and the developed faults are likely the key factors for the formation of intermediate to evolved monogenetic volcanos in the Touxi volcanic field.

Nonflammable Ether and Phosphate-Based Liquid Electrolytes for Sodium-Ion Batteries.

This study investigates a group of electrolytes containing NaPF6 or NaBF4 salts in phosphate- and ether-based solvents for high-mass loading sodium-ion batteries. It explores physicochemical properties such as ionic conductivity, dynamic viscosities, and nonflammability. The combination of experimental with computational studies reveals detailed insights into the physicochemical properties of the nonflammable liquid electrolytes. Diglyme-based electrolytes become nonflammable with 50 vol % phosphate solvents, while tetraglyme-based electrolytes require 70 vol %. The solvation structure has been investigated using NMR and is combined with computational studies to provide information about properties such as solvation structure, ionic conductivity, and viscosity. The molecular dynamic simulations confirm the enhanced solvation in diglyme-based liquid electrolytes observed experimentally by 23Na-NMR. Despite lacking sufficient electrochemical stability, this work provides a fundamental understanding of the solvation structure and physicochemical properties of a novel electrolyte system. This is an important contribution to be applied in future electrolyte design rationale.

Predicting visual function by interpreting a neuronal wiring diagram

As connectomics advances, it will become commonplace to know far more about the structure of a nervous system than about its function. The starting point for many investigations will become neuronal wiring diagrams, which will be interpreted to make theoretical predictions about function. Here I demonstrate this emerging approach with the Drosophila optic lobe, analysing its structure to predict that three Dm3 (refs. 1–4) and three TmY (refs. 2,4) cell types are part of a circuit that serves the function of form vision. Receptive fields are predicted from connectivity, and suggest that the cell types encode the local orientation of a visual stimulus. Extraclassical5,6 receptive fields are also predicted, with implications for robust orientation tuning7, position invariance8,9 and completion of noisy or illusory contours10,11. The TmY types synapse onto neurons that project from the optic lobe to the central brain12,13, which are conjectured to compute conjunctions and disjunctions of oriented features. My predictions can be tested through neurophysiology, which would constrain the parameters and biophysical mechanisms in neural network models of fly vision14.

Neural relational and dynamics inference for complex systems

Many complex processes in the real world can be viewed as complex systems and their evolution is governed by underlying nonlinear dynamics. However, one can only access the trajectories of the system without knowing the underlying system structure and dynamics in most cases. To address this challenge, this paper proposes a model called Neural Relational and Dynamics Inference (NRDI) that combines graph neural networks (GNNs) and ordinary differential equation systems (ODEs) to handle both continuous-time dynamics prediction and network topology inference for complex systems. Our model contains two modules: (1) the network inference module, which infers system structure from input system trajectories using GNNs, and (2) the dynamics learning module, which employs GNNs to fit the differential equation system for predicting future trajectories. We tested NRDI’s performance on system trajectory prediction and graph reconstruction separately. Experimental results show that the proposed NRDI outperforms many baseline models on continuous-time complex network dynamics prediction, and can explicitly infer network structures with high accuracy.

The meaning of modern neuroanatomy

BackgroundNeuroanatomy, the study of the structure and organization of the nervous system, has developed gradually to become a cornerstone of neurological sciences. As understanding of the brain and its intricate networks continues to evolve, modern neuroanatomy integrates advanced technologies and multidisciplinary approaches to provide deeper insights into the complexities of the human brain. AimThis report aims to present the meaning of modern neuroanatomy, its tools, applications, and the future perspectives. ConclusionsThe data gained from modern neuroanatomy have profound implications for understanding brain function, diagnosing and treating neurological disorders, and addressing ethical and societal challenges.

Important role of Chloroflexi: Improved the cooperation of anammox community under PFOS stress

Perfluorooctane sulfonic acid (PFOS) has received extensive attention in recent years due to its persistence and biotoxicity. As an advanced biological nitrogen removal process, anaerobic ammonium oxidation (anammox) is considered to be the most promising process to replace traditional nitrogen removal technology in the future. However, it is still unclear how PFOS affects microbial community and network structure in anammox system. The study found that PFOS had little influence on the nitrogen removal efficiency. Through microbial community and network analysis, the results showed that the typical anammox bacteria (Candidatus_Kuenenia, Candidatus_Jettenia, and Candidatus_Brocadia) enriched with the increasing concentrations of PFOS. Overall network complexity and proportion of positive links was significantly increased under 10 mg/L PFOS. Especially the subnetwork of Chloroflexi owned 100 % positive links under 10 mg/L PFOS, which accounted for 53.62 % of the corresponding overall network. The genus OLB13 and norank_f__Anaerolineaceae from Chloroflexi could cooperate with anammox bacteria to resist the impact of PFOS and further maintained the stability of anammox system. This study provided new insights into the effects of perfluoroalkyl compounds on anammox system.