Structure Of Extracellular Polymeric Substances Research Articles

The Impact of Bisphenol A on the Anaerobic Sulfur Transformation: Promoting Sulfur Flow and Toxic H2S Production.

Bisphenol A (BPA), as a typical leachable additive from microplastics and one of the most productive bulk chemicals, is widely distributed in sediments, sewers, and wastewater treatment plants, where active sulfur cycling takes place. However, the effect of BPA on sulfur transformation, particularly toxic H2S production, has been previously overlooked. This work found that BPA at environmentally relevant levels (i.e., 50-200 mg/kg total suspended solids, TSS) promoted the release of soluble sulfur compounds and increased H2S gas production by 14.3-31.9%. The tryptophan-like proteins of microbe extracellular polymeric substances (EPSs) can spontaneously adsorb BPA, which is an enthalpy-driven reaction (ΔH = -513.5 kJ mol-1, ΔS = -1.60 kJ mol-1K -1, and ΔG = -19.52 kJ mol-1 at 35 °C). This binding changed the composition and structure of EPSs, which improved the direct electron transfer capacity of EPSs, thereby promoting the bioprocesses of organic sulfur hydrolysis and sulfate reduction. In addition, BPA presence enriched the functional microbes (e.g., Desulfovibrio and Desulfuromonas) responsible for organic sulfur mineralization and inorganic sulfate reduction and increased the abundance of related genes involved in ATP-binding cassette transporters and sulfur metabolism (e.g., Sat and AspB), which promoted anaerobic sulfur transformation. This work deepens our understanding of the interaction between BPA and sulfur transformation occurring in anaerobic environments.

Mechanistic insight into the aggregation ability of anammox microorganisms: Roles of polarity, composition and molecular structure of extracellular polymeric substances

The chemical characteristics of extracellular polymeric substances (EPS) of anammox bacteria (AnAOB) play a crucial role in the rapid enrichment of AnAOB and the stable operation of wastewater anammox processes. To clarify the influential mechanisms of sludge EPS on AnAOB aggregation, multiple parameters, including the polarity distribution, composition, and molecular structure of EPS, were selected, and their quantitative relationship with AnAOB aggregation was analyzed. Compared to typical anaerobic sludge (anaerobic floc and granular sludge), the anammox sludge EPS exhibited higher levels of tryptophan-like substances (44.82–56.52 % vs. 2.57–39.81 %), polysaccharides (40.02–53.49 mg/g VSS vs. 30.22–41.69 mg/g VSS), and protein structural units including α-helices (20.70–23.98 % vs. 16.48–19.32 %), β-sheets (37.43–42.98 % vs. 25.78–36.72 %), and protonated nitrogen (Npr) (0.065–0.122 vs. 0.017–0.061). In contrast, it had lower contents of β-turns (20.95–27.39 % vs. 28.17–39.04 %). These biopolymers were found to originate from different genera of AnAOB. Specifically, the α-helix-rich proteins were mainly derived from Candidatus Kuenenia, whereas the extracellular proteins related to tryptophan and Npr were closely associated with Candidatus Brocadia. Critically, these EPS components could drive anammox aggregation through interactions. Substantial amounts of tryptophan-like substances facilitated the formation of β-sheet structures and the exposure of internal hydrophobic clusters, which benefited the anammox aggregation. Meanwhile, extracellular proteins with high Npr content played a pivotal role in the formation of mixed protein-polysaccharide gel networks with the electronegative regions of polysaccharides, which could be regarded as the key component in the maintenance of anammox sludge stability. These findings provide a comprehensive understanding of the multifaceted roles of EPS in driving anammox aggregation and offer valuable insights into the development of EPS regulation strategies aimed at optimizing the anammox process.

Ultrahigh pressure filtration dewatering of municipal sludge conditioned by steel slag in combination with Fe2+/ sodium persulfate

Municipal sludge treatment in urban contexts faces significant challenges, especially when dealing with sludge characterized by exceptionally high water content and organic content. This study investigates the application of sodium persulfate oxidation activated by ferrous ions (Fe2+/SPS) in conjunction with waste steel slag as a conditioning agent for municipal sludge, capillary suction time and specific resistance filtration experiments were conducted on conditioned sewage sludge, along with dewatering tests under ultrahigh pressure conditions. Additionally, to investigate the potential underlying mechanisms, Scanning Electron Microscopy analysis, Zeta potential measurements, and comprehensive sample analyses were performed. The research reveals substantial improvements in sludge dewatering performance when steel slag is combined with Fe2+/SPS treatment. This combined treatment introduces additional ferrous ions into the sludge, generating radicals that disrupt the structure of extracellular polymeric substances and cells within the sludge. Steel slag also acts as a skeleton builder material, creating larger drainage pathways within the sludge cake under ultrahigh pressure, enhancing permeability, and accelerating moisture release. This combined treatment method holds promise for achieving deep sludge dewatering, under ultrahigh filtration pressure, with water content reduced to below 40%. The study not only highlights the advantages of using steel slag in combination with Fe2+/SPS treatment for sludge dewatering but also provides insights into the underlying mechanisms. This comprehensive treatment method reduces the excessive use of ferrous ions and costs while significantly enhancing dewatering performance, offering a promising solution for municipal sludge treatment and management.

Analysis of the Effects of Surfactants on Extracellular Polymeric Substances

Reservoirs after chemical flooding usually have residual chemicals, which can affect the driving effect of subsequent microbial drives. Among them, the effect of surfactants on the metabolites of oil-recovering bacteria is the most obvious. Therefore, this paper investigates the influence mechanism of sodium dodecyl sulfate (SDS) on the nature and structure of Extracellular Polymeric Substances (EPS) produced by metabolism of Enterobacter cloacae, through a variety of characterization to analysis the components and structure of EPS under SDS stress. The results showed that Enterobacter cloacae was identified as a glycolipid-producing strain, the main components of EPS were polysaccharides and proteins. The polysaccharide composition (%: w/w) was glucosamine, 37.2; glucose, 31.5; rhamnose, 26.3; xylose, 1.7; and unidentified sugar, 3.3; and the main component of proteins was polyglutamic acid. EPS under the stress of SDS showed an increase in the content of functional groups such as -C=O and -COOH and an increase in the cellular particle size, and production of EPS increased by 10.69 × 103 mg/L when the SDS concentration was 2.5 × 102 mg/L; 3D-EEM results showed that the components of all three types of EPS The 3D-EEM results showed that all three types of EPS fractions contained tryptophan and protein-like substances, humic acid-like substances were only distributed in the solubilized extracellular polymers (SL-EPS), and aromatic proteins were only present in the loosely bound type (LB-EPS) and tightly bound type (TB-EPS). In addition, the peaks representing humic-like substances showed a blue shift, indicating that SDS had the greatest effect on SL-EPS. This study provides a guidance for refining the mechanism of strain EPS response to reservoir residual surfactant SDS, and provides a more comprehensive and in-depth understanding of surfactant-protein interactions.

Effects of feeding mode on the formation and stability of aerobic granular sludge under combined antibiotic stress

Aerobic granular sludge (AGS) is a promising technology for wastewater treatment due to its high biomass retention, nutrient removal, and resistance to toxic substances. However, the formation and stability of AGS are affected by various factors, such as feeding mode and antibiotic stress. This study investigated how three feeding modes, namely anaerobic plug-flow (APM), anaerobic mixed-flow (AMM), and aerobic feeding mode (AFM), influenced AGS formation and stability under exposure to a mixture of tetracycline, sulfamethoxazole, ofloxacin, and roxithromycin. The results showed that only APM and AMM achieved granulation within 40 d, while AFM failed to form AGS within 90 d due to the low shear force that led to a different composition and structure of extracellular polymeric substances and a high potential barrier calculated by extended Derjaguin-Landau-Verwey-Overbeek (XDLVO) theory. The average granules diameters of APM (896.6 μm) were larger than that of AMM (519.4 μm), and the results of ultrasonic crushing experiment and XDLVO calculation showed that the structural stability of APM was slightly stronger. However, AMM outperformed APM and AFM in terms of total phosphorus removal, antibiotic removal, and long-term operational stability under low temperature conditions. Illumina MiSeq sequencing and qPCR results revealed that typical biofilm formers (Sediminibacterium and env.OPS_17) contributed to the stability of AGS and increased phosphorus accumulating organisms’ genes abundance ensured the high TP removal in AMM. This study offers valuable insights into the effects of diverse feeding modes on aerobic granulation and stability under combined antibiotic stress and suggests that AMM is a promising mode for enhancing AGS performance and resilience in wastewater treatment applications.

Surfactant and antibiotic co-occurrence reshaped the acidogenic process for volatile fatty acids production during sludge anaerobic fermentation

The overuse of surfactants and antibiotics has led to their high concentration in waste activated sludge (WAS), and these exogenous pollutants have been shown to pose various influences on the subsequent anaerobic treatment process. Previous works have primarily concerned the impacts of individual pollutants on WAS anaerobic fermentation process. This work revealed the synergetic effects of sodium dodecyl benzene sulfonate (SDBS) and sulfadiazine (SDZ) co-occurrence in WAS on the biosynthesis of volatile fatty acids (VFAs). The addition of SDBS in the SDZ reactor significantly increased VFAs generation, and this increase was correlated with the concentration of SDZ. The VFAs production exhibited a 200.0–211.9 % and 5.9–20.4 % increase in comparison with the sole SDZ and SDBS reactor, respectively. The SDBS and SDZ co-occurrence facilitated the solubilization, hydrolysis, and acidification stages of WAS fermentation synchronously. SDBS was effectively to disintegrate the cemented structure of extracellular polymeric substances and meanwhile improve the SDZ solubilization, which increase the SDZ bioavailability as well as biotoxicity to the anaerobic species. Herein, the anaerobic consortia structure was evidently reshaped, and the keystone microbes Acetoanaerobium and Fususibacter, as well-tolerated hydrolytic-acidogenic bacteria, were greatly enriched. Furthermore, the functional microbial metabolic traits responsible for the substrate extracellular hydrolysis (e.g., glsA and MAN2C1), intracellular metabolism (e.g., ALDO and asdA), and fatty acid generation (e.g., aarC) were all upregulated in the SDBS/SDZ co-occurrence reactor.

Enhanced dewaterability of sewage sludge by grafted cationic lignin-based flocculants

Lignin-based flocculants are widely used for wastewater purification, but their application in sludge dewatering has not yet been documented. In this study, a novel cationic lignin-based flocculant named LS-g-CPA was prepared by grafting cationic polyacrylamide (CPA) synthesized from methacryloyloxy ethyltrimethyl ammonium chloride (DMC) and acrylamide (AM) onto sodium lignosulfonate (LS), and its roles and underlying mechanisms in sludge conditioning were investigated. The results showed that LS-g-CPA effectively improved the dewaterability of sludge, reducing the filtration resistance and filter cake moisture content of sludge from 0.61 ± 0.05 × 1012 m/kg to 0.14 ± 0.02 × 1012 m/kg and 85.64 ± 0.25 % to 76.84 ± 0.41 %, respectively. The dewatering performance of LS-g-CPA was positively correlated with the DMC/AM ratio. The quaternary ammonium groups brought by DMC disrupted the reticular structure of extracellular polymeric substances, exposing hydrophobic residues and releasing bound water. Nevertheless, the key to LS-g-CPA for improving sludge dewatering lies more in the amphoteric flocculant properties that enhance sludge flocculation and the octopus-type structure that provides good drainage channels. This study reveals that lignin-based flocculants are effective in improving the dewaterability of sludge, which provides direct evidence for their application in sludge dewatering.

Multifaceted roles of methylisothiazolinone intervention in sludge disintegration and acidogenic and methanogenic pathways for efficient carboxylate production during anaerobic fermentation

Anaerobic fermentation is a promising approach for sludge treatment, although it is frequently hindered by the tight structure of extracellular polymeric substances (EPS) and limited metabolic activities. This work firstly discovered that methylisothiazolinone (MIT, a commonly used antimicrobial agent in water treatment) effectively disintegrated the sludge structure and stimulated metabolic pathways, leading to efficient volatile fatty acids (VFAs) production. The presence of MIT (ranging from 10 to 40 mg/g TSS) contributed to the acidogenic process but strongly restrained the methanogenesis, and the distinct interfering effect is highly associated with the MIT dose. The VFAs accumulation reached a maximum of 1776.7 mg COD/L (5.2 times of control) but faced a complete inhibition of methanogenic activity at 40 mg/g TSS. Spectroscopic and molecular docking analysis revealed that MIT interacted with and disrupted the EPS (mainly proteins) structure by forming hydrogen bonds with N-H groups. This disruption facilitated the release of biodegradable organics, which served as fermentation substrates. Furthermore, the MIT-mediated improvement selectively enriched the hydrolytic-acidifying bacteria (i.e., Clostridium and Caloramator) and stimulated the associated metabolic functions, particularly in the uptake, hydrolysis, and metabolism of amino acids considering the high expressions of encoding genes (e.g., oppA, pepP, gdhA). However, methanogens' reproductive and metabolic abilities were suppressed due to their inefficient response and adaptation to MIT. This suppression was evident through downregulated genetic expressions involved in DNA replication and aminoacyl-tRNA synthesis. Consequently, a decline in methanogens abundance (e.g., Methanothrix, and Methanosarcina) and methanogenic activity occurred, impeding the consumption of VFAs. This work provided an innovative niche to promote resource recovery in the sludge anaerobic system and deciphered the underlying mechanisms at both biochemical interactive steps and microbial genetic traits.

Earthworms restructure the distribution of extracellular antibiotics resistance genes of sludge by modifying the structure of extracellular polymeric substances during vermicomposting

The role of earthworms in reducing the antibiotic resistance genes (ARGs) in sludge vermicompost remains unclear. The structure of extracellular polymeric substance (EPS) of sludge may be associated with the horizontal gene transfer behavior of ARGs in the vermicomposting of sludge. Therefore, this study aimed to investigate the effects of earthworms on the structural characteristics of EPS associated with the fate of ARGs in EPS during the vermicomposting of sludge. The results showed vermicomposting could diminish the abundance of ARGs and mobile genetic elements (MGEs) in the EPS of sludge by 47.93 % and 7.75 %, compared to the control, respectively. Relative to the control, vermicomposting also led to the reduction of MGEs abundances in the soluble EPS of 40.04 %, lightly bound EPS of 43.53 %, and tightly bound EPS of 70.49 %, respectively. The total abundances of certain ARGs dramatically diminished 95.37 % in tightly bound EPS of sludge during vermicomposting. In vermicomposting, the main influencing factor of ARGs distribution was the proteins in LB-EPS, accounting for 48.5 % of the variation. This study suggests that the earthworms lower the total abundances of ARGs by regulating the microbial community and modifying the microbial metabolic pathways associated with ARGs and MGEs in the EPS of sludge.

Physiological Responses of Methanosarcina barkeri under Ammonia Stress at the Molecular Level: The Unignorable Lipid Reprogramming.

Acetotrophic methanogens' dysfunction in anaerobic digestion under ammonia pressure has been widely concerned. Lipids, the main cytomembrane structural biomolecules, normally play indispensable roles in guaranteeing cell functionality. However, no studies explored the effects of high ammonia on acetotrophic methanogens' lipids. Here, a high-throughput lipidomic interrogation deciphered lipid reprogramming in representative acetoclastic methanogen (Methanosarcina barkeri) upon high ammonia exposure. The results showed that high ammonia conspicuously reduced polyunsaturated lipids and longer-chain lipids, while accumulating lipids with shorter chains and/or more saturation. Also, the correlation network analysis visualized some sphingolipids as the most active participant in lipid-lipid communications, implying that the ammonia-induced enrichment in these sphingolipids triggered other lipid changes. In addition, we discovered the decreased integrity, elevated permeability, depolarization, and diminished fluidity of lipid-supported membranes under ammonia restraint, verifying the noxious ramifications of lipid abnormalities. Additional analysis revealed that high ammonia destabilized the structure of extracellular polymeric substances (EPSs) capable of protecting lipids, e.g., declining α-helix/(β-sheet + random coil) and 3-turn helix ratios. Furthermore, the abiotic impairment of critical EPS bonds, including C-OH, C═O-NH-, and S-S, and the biotic downregulation of functional proteins involved in transcription, translation, and EPS building blocks' supply were unraveled under ammonia stress and implied as the crucial mechanisms for EPS reshaping.

Enhanced methane production from anaerobic digestion of waste activated sludge by combining ultrasound with potassium permanganate pretreatment.

The influence of ultrasound (US) and potassium permanganate (KMnO4) co-pretreatment on anaerobic digestion of waste activated sludge (WAS) was investigated in this survey. Results showed that KMnO4 (0.3 g/g TSS) cooperated with US (1 W/mL, 15 min) pretreatment significantly increased the cumulative methane yield to 174.44 ± 3.65 mL/g VS compared to the control group (108.72 ± 2.56 mL/g VS), solo US (125.39 ± 2.56 mL/g VS), and solo KMnO4 pretreatment group (160.83 ± 1.61 mL/g VS). Mechanistic investigation revealed that US combined with KMnO4 pretreatment effectively disrupted the structure of extracellular polymeric substances and cell walls by generating reactive radicals, accelerating the release of organics and hydrolytic enzymes as well as improving the biodegradability of soluble organics. Modeling analysis illustrated that the biochemical methane potential and hydrolysis rate of WAS were enhanced under US + KMnO4 pretreatment. Microbial community distribution indicated that the co-pretreatment of US and KMnO4 elevated the total relative abundance of functional microorganisms associated with anaerobic digestion (22.01 %) compared to the control (10.69 %), US alone (12.24 %) and KMnO4 alone (16.20 %).

Enhanced photodegradation of antibiotics based on anoxygenic photosynthetic bacteria and bacterial metabolites: A sustainably green strategy for the removal of high-risk organics from secondary effluent

Antibiotic residues in effluents discharged from wastewater treatment plants (WWTPs) have been considered high-risk organics due to biorefractory property and potential toxicity. Secondary pollution and unsustainability existed in advanced treatment of secondary effluent are currently in urgent need of improvement. In this study, a sustainably green strategy based on Rhodopseudomonas palustris (R.palustris) by regulating the structure of extracellular polymeric substances (EPS) was proposed for the first time to achieve efficiently removal of sulfadiazine (SDZ). Results showed that 0.2 V was the optimal external potential for R.palustris to efficiently remove SDZ, where the biodegradation rate constant obtained at this potential was 4.87-folds higher than that in open-circuit mode and a complete removal was achieved within 58 h in the presence of EPS extracted at this potential. Three-dimensional excitation-emission matrix (3D-EEM) spectra analysis suggested that tryptophan protein-like, tyrosine protein-like, humic acid-like and fulvic acid-like substances present in EPS were the main effective components which was responsible for the indirect photodegradation of SDZ. The quenching experiments showed that 3EPS* was the dominant reactive species which accounted for 90% of SDZ removal. This study provides new implications for the advanced treatment of secondary effluent organic matters by developing eco-friendly bioaugmentation technology and biomaterials.

Hydrolysis kinetics for solubilizing waste activated sludge at low temperature thermal treatment derived from multivariate non-linear model

Low temperature thermal pre-treatment is a low-cost method to break down the structure of extracellular polymeric substances in waste activated sludge (WAS) while improving the sludge biodegradability. However, previous models on low temperature thermal pre-treatment did not adequately elucidate the behaviour of sludge hydrolysis process for the duration ranging from 5 to 9 h. Therefore, this work had developed an inclusive functional model to describe the kinetics of sludge hydrolysis for a wide range of treatment conditions (30 °C–90 °C within 0 and 16 h). As compared with treatment duration, the treatment temperature played a greater impact in solubilizing WAS. Accordingly, the 90 °C treatment had consistently produced WAS with the highest degree of solubility. Nonetheless, the mediocre discrepancies between 90 °C and 75 °C may challenge the practicality of increasing the treatment temperatures beyond 75 °C. The effects of treatment duration on soluble chemical oxygen demand, soluble carbohydrate and soluble protein were only significant during the first 4 h, except for humic substances release that continued to increase with treatment duration. Finally, a good fit with R2 > 0.95 was achieved using an inclusive multivariate non-linear model, substantiating the functionality to predict the kinetics of sludge hydrolysis at arbitrary treatment conditions.

Responses of electroactive biofilms to chronic chlorine exposure: Insights from the composition and spatial structure of extracellular polymeric substances

Extensive amounts of chlorine disinfectants have been applied to wastewater system since the outbreak of coronavirus disease 2019 (COVID-19), which inevitably affects the pollutant degradation via interfering with electron transfer mediated by electroactive bacteria. Herein, the response of electroactive biofilm (EAB) to chronic chlorine exposure was investigated. Results showed the EAB formed without exposure (EAB-0) exhibited a 53% and 123% higher current output than that formed with 0.1 mg L−1 (EAB-0.1) and 0.5 mg L−1 (EAB-0.5) chlorine, respectively. The chronic chlorine exposure of EAB boosted the contents of extracellular polymeric substances (EPS) in EAB-0.1 and EAB-0.5 by over secretion of extracellular polysaccharides. The EAB-0.1 and EAB-0.5 also presented lower electron exchange capacities (EECs) of EPS, coincided with reduced relative abundance of Geobacter from 61% in EAB-0 to 52% in EAB-0.5. This study provided new insights into the application of engineered EAB for wastewater treatment in a disinfection environment.

Changes in molecular structure of extracellular polymeric substances (EPS) with temperature in relation to sludge macro-physical properties

Extracellular polymeric substances (EPS) are important components of activated sludge, whose content and composition have important effects on the macro-physical properties of sludge. In this study, the response of EPS in sludge to temperature (−40–200 °C) was systematically investigated using XAD resin fractionation, variable-temperature infrared spectra (VTIS) and two-dimensional correlation spectroscopy (2D-COS). The relationships between the molecular structure of EPS and the macro-physical properties (rheological property and dewatering performance) of waste activated sludge (WAS) at varying temperature were also established. During the freezing treatment, the solubilization of biopolymers and destruction of the hydrophilic functional groups (hydroxy, amino and carboxyl) resulted in the production of small organic matters, which enhanced EPS hydrophobicity and reduced electrostatic repulsion of sludge, and subsequent dewaterability improvement. For the hydrothermal treatment, the EPS transformation showed a two-stages reaction including stage I (70–120 °C) and stage II (>120 °C). Stage I (70–120 °C), a plenty of hydrophilic functional groups (hydroxy, amino and carboxyl) in EPS were exposed via the solubilization of biopolymers, which enhanced electrostatic repulsion of sludge and EPS hydrophilicity, and subsequence in deterioration of sludge dewaterability and fluidity. However, at stage II (>120 °C), the high temperature caused hydrolyzation of macromolecular organic matters in completely, in which the secondary structure of the protein was destroyed, causing the peptide chain to unfold. In addition, the reduction of α-helix and β-sheet content and intensified Maillard reaction decreased electrostatic repulsion of sludge, thus resulted in the improvement of sludge dewaterability and fluidity. This study enriched the theoretical basis of the optimal control of sludge treatment based on temperature regulation.

Pyrite assisted peroxymonosulfate sludge conditioning: Uncover triclosan transformation during treatment

Waste activated sludge (WAS) dewatering is a crucial process for sludge treatment and disposal. In this study, we proposed a novel pyrite (FeS2) and peroxymonosulfate (PMS) treatment to improve WAS dewaterability. Micropollutants are commonly enriched in the sludge. It is not clear if the micropollutants remain in the sludge during the conditioning. Triclosan (TCS) as a widely used bactericide often presents in the WAS, thus was chosen as a target micropollutant. Pyrite + PMS treatment could simultaneously enhance WAS dewaterability and TCS removal with low cost and high benefit. Under the optimal conditions, the specific resistance of filtration (SRF) and capillary suction time (CST) were reduced by 84.60% and 74.91%, respectively. Meanwhile, the TCS removal efficiency was 34.08% with four transformation products identified. During the pyrite + PMS process, sulfate radicals and hydroxyl radicals were generated and strong flocculation was induced by iron. These two processes significantly reduced the sticky biopolymers, hydrophilic functional groups, and hydrophilic protein molecular structure of extracellular polymeric substances (EPS), leading to the release of bound water and TCS. Collectively, the pyrite + PMS treatment is a promising alternative for simultaneous enhancement of WAS dewatering and micropollutants removal, which is beneficial to the downstream treatment.

MnTE-2-PyP disrupts Staphylococcus aureus biofilms in a novel fracture model.

Biofilm-associated infections in orthopedic surgery lead to worse clinical outcomes and greater morbidity and mortality. The scope of the problem encompasses infected total joints, internally fixed fractures, and implanted devices. Diagnosis is difficult. Cultures are often negative, and antibiotic treatments areineffective. The infections resist killing by the immune system and antibiotics.The organized matrix structure of extracellular polymeric substances within the biofilm shields and protects the bacteria from identification and immune cell action. Bacteria in biofilms actively modulate their redox environment and can enhance the matrix structure by creating an oxidizing environment.We postulated that a potent redox-active metalloporphyrin MnTE-2-PyP (chemical name: manganese (II) meso-tetrakis-(N-methylpyridinium-2-yl) porphyrin) that scavenges reactive species and modulates the redox state to a reduced state, would improve the effect of antibiotic treatment for a biofilm-associated infection. An infected fracture model with a midshaft femoral osteotomy was created in C57B6 mice, internally fixed with an intramedullary 23-gauge needle and seeded with a biofilm-forming variant of Staphylococcusaureus. Animals were divided into three treatment groups: control, antibiotic alone, and combined antibioticplus MnTE-2-PyP. The combined treatment group had significantly decreased bacterial counts in harvested bone, compared with antibiotic alone. In vitrocrystal violet assay of biofilm structure and corresponding nitroblue tetrazolium assay for reactive oxygen species (ROS) demonstrated that MnTE-2-PyP decreased the biofilm structureand reduced ROS in a correlated and dose-dependent manner. The biofilm structure is redox-sensitive in S. aureus and an ROS scavenger improved the effect of antibiotic therapy in model of biofilm-associated infections.

The disintegration of excess sludge enhanced by short-term interaction with potassium ferrate: Characteristics and mechanism

The increasing amount of excess sludge (ES) has become a heavy burden for biological wastewater treatment, and the function of potassium ferrate (PF) in ES treatment as a strong oxidizing reagent has received increasing attention. This study comprehensively investigated the contribution of different dosages of PF on sludge disintegration in a short time interaction with sludge, from the perspective of the rheological properties, adhesion ability, and microbial mortality rate of sludge. The results of electrochemical impedance spectroscopy found that the adhesion ability of sludge could greatly decrease to 6.9–30% of the initial one by PF treatment, and the results of the rotational rheometer revealed that the cross-linked structure of extracellular polymeric substances could be destructed by the oxidation characteristics of PF (ES/PF<15:3), resulting in the transformation of the sludge from gel to sol structure. Moreover, with the increasing dosage of PF (ES/PF<60:1), the microbial mortality rate in sludge could increase rapidly from an initial 17.3 to 50.4%, and as a result of the above reason, large amounts of biomacromolecules and phosphate were released into the liquid phase. These results concluded that PF could preferentially deconstruct the EPS structure in sludge, and then the excess PF could damage the cell membrane, which results in the release of intracellular materials, and eventually, ES was totally disintegrated. This study provided an investigation to understand the function of PF in the sludge disintegration and a reference for the application of PF in a mass reduction of sludge in a short time.

Role of extracellular polymeric substances in anaerobic granular sludge: Assessing dewaterability during Fe(II)-peroxydisulfate conditioning and granulation processes

In this study, Fe(II) activated peroxydisulfate (PDS) conditioning and sludge granulation were conducted to investigate the dewaterability of anaerobic granular sludge (AGS). After Fe(II)-PDS conditioning, the dewaterability of three AGS from different sources was enhanced. The specific resistance to filtration (SRF) reduction rates were achieved (98.30% ± 0.19%, 99.51% ± 0.17% and 96.47% ± 1.25%, respectively) under the optimal Fe(II) and PDS additions; And the optimal reductions of capillary suction time (CST) were 93.49% ± 2.49%, 95.33% ± 0.02% and 88.04% ± 2.95%, respectively. The mechanism of improving AGS dewaterability by Fe(II)-PDS conditioning was proposed. The radical SO4⋅−/OH⋅ destroyed the structure of extracellular polymeric substances (EPS) layers and microbial cells, resulting in the bound water released from AGS. Thereafter, the generated Fe(III) facilitated the sludge re-flocculation and decreased the electrostatic repulsion. During a 132-day granulation, the CST value showed a positive correlation with protein (S-EPS), polysaccharide and zeta potential, and a negative correlation with protein (LB-EPS), protein (TB-EPS), particle size and VSS. Collectively, the protein was the primary component in AGS and showed a strong correlation with dewaterability. The variations of protein in TB-EPS during the conditioning and the granulation were consistent with the changes of sludge dewaterability.

Evaluation of the combined effect of sodium persulfate and thermal hydrolysis on sludge dewatering performance.

This innovative study makes use of a thermal hydrolysis process (THP) and the conditioner sodium persulfate (SPS) to improve the dewaterability of sewage sludge. The best-operating conditions were optimized using response surface methodology (RSM): 100mg/g of dry solids (DS) of SPS, 101min of reaction time of THP, and a temperature of 200°C. Distribution of extracellular polymeric substances (EPS), zeta potential, bound water, and solid characters were analyzed to reveal the mechanisms involved in the dewatering process. These results indicate that the sewage sludge after treatment (SPS combined with THP) had a superior dewaterability. The specific resistance to filtration (SRF) under the best conditions was 0.51 × 1011m/kg, decreasing by 91.65% compared to the raw sludge (RS) (6.11 × 1011m/kg). This mechanism could be explained as follows: (1) Aromaticity and hydrophobicity of sludge cake after SPS + THP treatment was increased; (2) sludge flocs were re-flocculated by charge neutralization, giving rise to a loose and porous structure; (3) the structure of extracellular polymeric substances and cells was destroyed, and the bound water was released. Overall, the conditioning by combination of SPS and THP is an effective mean to improve sewage sludge dewaterability. Graphical abstract.