Abstract. Daily transpiration (Td) is crucial for both irrigation water management and increasing crop water productivity. The use of the remote-sensing-based two-source energy balance model (TSEB) has proven to be robust in estimating plant transpiration and evaporation separately for various crops. However, remote sensing models provide instantaneous estimations, and so daily upscaling approaches are needed to estimate daily fluxes. Daily upscaling methodologies have not yet been examined to upscale solely transpiration in woody crops. In this regard, this study aims to evaluate the proper image acquisition time throughout the day and four methodologies used to retrieve Td in almond trees with different production systems and water statuses. Hourly transpiration (Th) was estimated using the TSEB contextual approach (Th–TSEB) with high-resolution imagery five times during two diurnal courses. The tested methodologies were the following: the simulated evaporative fraction variable (EFsim), irradiance (Rs), reference evapotranspiration (ETo), and potential evapotranspiration (ETp). These approaches were first evaluated with in situ sap flow (T–SF) data and were then applied to the Th–TSEB. Daily T–SF showed significant differences among production systems and levels of water stress. The EFsim and ETp methods correlated better with measured T–SF and reduced the underestimation observed using the Rs and ETo methods, especially at noon in the severely water-stressed trees. However, the daily upscaling approaches applied in the TSEB (Td–TSEB) failed to detect differences between production systems. The lack of sensibility of Th–TSEB among production systems poses a challenge when estimating Td in canopies with discontinuous architectural structures. The use of ETp as a reference variable could address this issue as it incorporates various aerodynamic and radiative properties associated with different canopy architectures that influence the daily Th–SF pattern. However, more accurate ETp estimates or more advanced ETp models are needed.