Strong Stressor Research Articles

Social stress in rats promotes transcriptional mitochondrial changes of the adrenal tissue

Previous observations suggest that strong social stressors, that is, repeated social defeat, could initiate functional changes in the hypothalamic-pituitary-adrenal (HPA) axis in social mammals. Here, we examine whether exposure to such stress in rats causes persistent alterations in gene expression associated with hypo-and/or hyper-methylation in the adrenal tissue, i.e., the interface between the HPA axis and the circulating hormones. Our integrated analyses of RNA-sequencing (RNAseq) and whole genome bisulfite sequencing (WGBS), suggested that adrenal mitochondrial membrane transport necessary for corticosterone (CORT) synthesis, are affected by repeated social defeat. The increased CORT levels observed in blood in the stressed rats further suggested that corticosterone synthesis might be influenced by this form of social stress. The cellular mechanisms underlying these changes, i.e., the enriched gene ontology (GO)-terms and increased levels of circulating CORT, remain to be investigated.

The Protective Role of Resilience on Cardiovascular Health in Young Adults Exposed to Adverse Childhood Experiences

Background: Adverse childhood experiences (ACEs) are strong emotional or physical stressors that occur during sensitive developmental periods and are associated with reduced cardiovascular health (CVH). Resilience, or the psychological capacity of individuals to cope with adversity, has been recently shown to have a positive influence on CVH. Despite the dose-dependent detrimental impact of ACEs on CVH, it is unknown whether resilience exerts a protective cardiovascular effect against early stress. Thus, the purpose of this study was to investigate the impact that resilience may have on CVH in young adults who experienced ACEs. Methods: Eighteen young and apparently healthy adults (22±3 yo) with a history of severe ACE exposure (≥4) participated in this study and provided self-reported data about resilience. CVH was assessed non-invasively through transcutaneous arterial tonometry to evaluate arterial stiffness (pulse-wave velocity, PWV), myocardial oxygen demand (Systolic Pressure Time Index, SPTI), myocardial oxygen supply (Diastolic Pressure Time Index, DPTI) and myocardial perfusion (Subendocardial Viability Ratio, SEVR). Circulating biomarkers related to CVH were also evaluated (high-sensitive C-reactive protein, hsCRP). Results: Eleven participants reported high resilience (HR: resilience score≥95), while seven reported low resilience (LR: resilience score<95). Young adults with high resilience presented with a significantly lower myocardial oxygen demand (SPTI; HR: 1866±111 vs. LR: 2227±117mmHg·s/min, p=0.047) and higher myocardial perfusion (SEVR; HR: 168±13 vs.LR: 127 ±9% , p=0.034) than those with low resilience and a positive association between SEVR and resilience was identified ( r=0.492; p=0.045). No differences in myocardial oxygen supply ( p=0.225) or arterial stiffness ( p=0.614) were observed between groups. However, circulating hsCRP was also significantly lower in the HR group than the LR (0.89±0.3 vs. 2.96±1 mg/dL, p=0.041) and negatively associated with resilience ( r=-0.511; p=0.051). Conclusions: Our data suggests that, among young and apparently healthy adults exposed to childhood adversity, those who are psychologically more resilient exhibit higher myocardial perfusion and overall better CVH than those less resilient despite a history of severe ACEs. These findings suggest that resilience may protect, at least partially, against the early impact of ACEs on CVH. Future studies are thus warranted to further explore the relationship between resilience and CVH. Supported in part by the Child Health Research Institute at VCU. This is the full abstract presented at the American Physiology Summit 2024 meeting and is only available in HTML format. There are no additional versions or additional content available for this abstract. Physiology was not involved in the peer review process.

Simulated microgravity induces DNA damage concurrent with impairment of DNA repair and activation of cell-type specific DNA damage response in microglial and glioblastoma cells

Long-term spaceflights affect the structural changes in brain, alter motor or cognitive function and associated development of neuro-optic syndrome in astronauts. Studies addressing the impact of microgravity on brain cells are very limited. Herein, we employed microglial (CHME3) and glioblastoma (U87MG and A172) cells to study their molecular and functional adaptations under simulated microgravity (SMG) exposure. A reduction in cell viability and proliferation with decreased levels of PCNA were observed in these cells. SMG caused extensive DNA damage with an increase in γH2A.X (ser139) phosphorylation and differential activation/expression of DNA damage response (DDR) proteins including ATM, ATR, Chk1, Chk2 and p53 in all the three cell lines. Unlike CHME3, the ATM/Chk2-dependent DDR pathway was activated in glioblastoma cells suggesting a marked difference in the adaptation between normal and cancer cells to SMG. Five different classes of DNA repair pathways including BER, NER, MMR, NHEJ and HR were suppressed in both cell lines with the notable exception of NHEJ (Ku70/80 and DNA-PK) activation in U87MG cells. SMG induced mitochondrial apoptosis with increased expression of Bax, cleaved caspase-3 and cleaved poly-(ADP-ribose) polymerase, and reduced Bcl-2 level. SMG triggered apoptosis simultaneously via ERK1/2 and AKT activation, and inhibition of GSK3β activity which was reversed by MEK1 and PI3K inhibitors. Taken together, our study revealed that microgravity is a strong stressor to trigger DNA damage and apoptosis through activation of ERK1/2 and AKT, and impairment of DNA repair capacity, albeit with a cell-type difference in DDR and NHEJ regulation, in microglial and glioblastoma cells.

Does intraspecific competition cause oxidative stress? Influence of biotic and abiotic factors on antioxidant system ofan invasive round goby.

Changes in oxidative status represent organismal response to stressful external stimuli. While there is substantial knowledge on the influence of abiotic factors on the antioxidant system of different organisms, the impact of biotic factors remains largely unexplored. The aim of the present study was to evaluate the effect of acute competitive interactions on oxidative stress. Territory-resident and intruder round goby Neogobius melanostomus individuals were experimentally subjected to competition for limited shelter resource in three treatments (lasting 1, 6 and 12 h), and oxidative stress parameters (total antioxidant capacity, catalase activity, reduced glutathione, lipid peroxidation), as well as behaviour (time spent in the shelter, guarding the shelter and aggression) were measured. All tested biochemical parameters reached higher values in the liver than in the muscle tissue. Fish behaviour and antioxidant defence did not show any potential relationships reflecting changes in antioxidant status and aggression. Particularly, there was no difference between resident and intruder fish in oxidative stress parameters. We compared our results to the outcome of our previous studies (similar experimental protocol and species) but with acute heat shock as a stressor instead of competition. The higher temperature was found to be a stronger stressor than the competition, most pronounced in total antioxidant capacity and oxidative damage.

Understanding the impact of physico-chemical parameters on aquatic invertebrates in Lake Chalco, Mexico City

Many urban environments are places with strong stressors that substantially modify water quality. Although tests evaluating water quality are usually physico-chemical, including biological components can also provide relevant information. Lake Chalco, at the border between Mexico City and Estado de México, interacts with the surrounding human population. We investigated some water quality parameters and the community of macroinvertebrates living in Lake Chalco. From January to October 2017, we sampled water along the shoreline in 4 permanent areas of the lake. We recorded the NH4+, NO3-N, NO2-N, total P, Zn, Cu2+, ORP, DO, TDS, conductivity, and pH. In addition, we characterized the richness, composition, and abundance of the macroinvertebrate community. There was spatial and temporal variation in physico-chemical parameters, perhaps due to the agricultural activity around the lake. Nevertheless, water quality was unable to be placed in a category of the national law. Four out of 20 macroinvertebrate RTU’s significantly correlated with environmental variables. Thus, no bioindicators could be proposed. In general, lake water quality is poor, so it is not recommended for anthropic activities. Yet, the lake may be important as a center of dispersion for aquatic invertebrates.

Overlapping exposure effects of pathogen and dimethoate on honeybee (Apis mellifera Linnaeus) metabolic rate and longevity.

Introduction: Declines in honeybee abundance have been observed worldwide during last decades. This is partly due to plant protection agents used in intensive farming, landscaping and infrastructure maintenance. Another type of factors negatively affecting honeybees is the spread of diseases caused by different pathogens and pests. Lately, more focus has been paid to the interactions between different overlapping stressors affecting honeybee health, the combination of these often being more detrimental compared to individual stressors. The most widely used stress-evaluating methods take into account lethal- or motorial changes of the individuals or colonies. Comparatively little honeybee research has examined changes in initial recovery potential and physiological symptoms of toxification. The aim of this study was to examine the combined effect of Nosema apis and N. ceranae (according to a newer classification Vairimorpha apis and V. ceranae), the common causes of nosemosis in the honeybee Apis mellifera L., with the insecticide dimethoate. Methods: In this study, honeybee mortality and metabolic rate were used to assess the combined effects interactions of Nosema ssp. and dimethoate. Results: Our results showed that exposure to the low concentration of either dimethoate, either one or both species of Nosema ssp as single factors or in the combination had no significant effect on honeybee metabolic rate. The mortality increased with the two Nosema spp., as well as with infection by N. ceranae alone. The effect of dimethoate was observed only in combination with N. apis infection, which alone had no effect on individual honeybee mortality. Conclusion: This study demonstrates that the overlapping exposure to a non-lethal concentration of a pesticide and a pathogen can be hidden by stronger stressor but become observable with milder stressors.

Structural-Functional Model of Musicians Performance Reliability in Concert And Stage Activity

The author of the article developed a structural-functional model of performance reliability of musicians in concert and stage activities and displayed it in a picture in its entirety. It is quite clear that the performance reliability of the performer is not innate, but formed by a property that provides favorable psychological conditions for the dominance of stimulating types of pop excitement ("excitement-elation" and "excitement in the image") over destructive ones ("excitement-fear", " excitement-panic" and "excitement-apathy"). Such dominance is also necessary for error-free sound realization of imaginatively created variable images of musical works using coordinated reproduction of performance skills. The components of performance reliability of musicians in concert and stage activities are determined and described by the author in accordance with their functional orientation. The author proves that the basis of this phenomenon consists of three interdependent macro-components — preparedness of the concert program, self-regulation of concert and stage activities, and interference resistance. Microcomponents of performance reliability of musicians in concert and stage activities are described taking into account the studied material. The preparedness of the concert program by musicians-performers depends on the quality of the interpretive model of musical works formed in the imagination, the perfection of the musician's technical equipment, the flexible ability to memorize material, information support and the automation of performance actions, the accuracy of muscle-force movements, their temporal relationship and sensory motor coordination. The functional orientation of the micro-components of self-regulation of concert and stage activity in the performance reliability of musicians is revealed, namely: self-control, self-evaluation, self-correction, attributes of control and their models. Four vectors of interference resistance of musicians-performers in concert and stage activities are described, where the first vector ensures the avoidance of the disorganizing influence of stressors on its course, the second - its leveling, the third - sensorimotor endurance of the excessive action of strong, sudden and long-lasting stressors, and the fourth - success resisting them. It has been proven that the resistance of musicians-performers in concert and stage activities depends on their general physiological stability, psychological stability, emotional stability, emotional-volitional stability and stress resistance

Living in Liquid Times: The Relationships among Job Insecurity, Life Uncertainty, and Psychosocial Well-Being.

Stress research has widely documented how uncertainty represents a strong stressor that, in general, is negatively associated with well-being. While the literature on job insecurity about this topic is extensive and exhaustive, empirical research on the outcomes of life uncertainty, namely the perception and feeling of precariousness regarding the present and future of one's own life, is yet to be fully explored. In the present paper, we aimed to investigate the relationships among job insecurity, life uncertainty, and psychosocial well-being outcomes, specifically, with a focus on job satisfaction and burnout. The participants were 357 workers (M = 146 and F = 211), with an average age of 41.78 y.o. (SD = 13.49), who completed an online questionnaire containing, in addition to sociodemographics information, measures of the study variables, namely job insecurity, life uncertainty, job satisfaction, and burnout. The results pointed out negative relationships of both job insecurity and life uncertainty with individual well-being, as they were negatively associated with job satisfaction and positively related to burnout. In a path analysis with latent variables, life uncertainty proved to fully mediate the relationship between job insecurity and psychosocial well-being.

Evaluation of Stress in Riding Horses, During the Horse-Assisted Therapy Activities in the Case of Children with Autism Spectrum Disorders


 Animal stress is a current topic intensely addressed. The general adaptation syndrome, following short exposure to stressors with low or medium intensity, has a positive effect, inducing eustress and improving adaptive capacity. Exposure to strong stressors or over a long period of time has the effect of inducing distress. The aim was to evaluate the stressful effect they have on horses, the specific activities of equine-assisted therapy, in the case of children with autism spectrum disorders. Were used 10 riding horses (8 females and 2 males), of purebred English breed, divided into 2 groups, aged between 4-8 years. The animals had the same maintenance conditions and lifestyle. Samples were taken from the jugular vein in 5 ml heparinized tubes. The parameters were determined using a ProCyte Dx Hematology Analyzer. The data obtained were statistically analyzed, calculating the Mean and Standard Deviation (Microsoft Excel). The results show that the specific activities of equine-assisted therapy do not have a stressful effect on the pathways used. These activities were less stressful than regular recreational riding activities in a riding school, subjecting the animal to less intense physical exertion than daily activities.

The positive aspects of caregiving in dementia: A scoping review and bibliometric analysis.

PurposeThe increasing incidence of dementia and home-based care exposes family caregivers to a variety of challenges as they endure strong stressors underlying the caregiver role. Despite growing publications on positive aspects of caregiving in dementia, few studies have identified the extent, nature, and gaps in the existing literature based on a holistic view. The aim was to identify key issues and a holistic view of literature regarding positive aspects of caregiving in dementia.MethodsA scoping review was conducted underlying a five-stage framework by Arksey and O'Malley. Five databases, including PubMed, CINAHL, PsycINFO, Embase, and Web of Science, were searched, and references were listed accordingly. Data were extracted by two researchers, comprising article characteristics, forms of positive aspects of caregiving and measurements, theories, forms of dementia and family caregiver, and keywords. Descriptive statistics and narrative synthesis were performed to analyze data. Network analysis of keywords and authors was conducted using VOSviewer software. Word cloud analysis of titles was examined by NVivo.ResultsThe review included 230 articles, most of which have been published in the last decade (62.61%). Most articles (40.00%) were contributed by the United States. Cross-sectional studies (41.30%) ranked first, followed by qualitative studies (13.48%). Over a quarter of the literature (28.26%) focused on Alzheimer's care, and nearly 90% included all forms of family caregivers. The Positive Aspects of Caregiving Scale and stress coping theory were most frequently cited. Four clusters dominated by Casey D, Quinn C, Joling KJ, and Teahan A were identified in the network of co-authorship. Six themes were identified: current situations of caregiver experiences, antecedents, consequences, measurement development, effects of interventions, and the concept of positive aspects of caregiving. These were in line with network analysis of keywords and word cloud analysis of titles.ConclusionsPositive aspects of caregiving in dementia have been widely concerned, but most of them are based on the theory of the negative stress process and are limited to current situations and influencing factors. Building theories focus on the positive aspects of caregiving, subsequently developing a comprehensive measurement and effective interventions, should be further studied.

Studi Kasus: Dinamika Gangguan Psikologis dan Fisik Yang Dialami Pria Pengurus Desa Adat Bali

Hipertension is dangerous disease that can take the life of the sufferer. Killing sufferers event without symptoms warns everyone to maintain physical health and also mental health. One of the factors that cause hypertension is stress. Including the figures or administrators of the Balinese Traditional Village. The workload is quite heavy and also voluntary (Ngayah) is a source of its own stressor. This Study aims to determine the psychological dynamic that occur in traditional leaders so that it can affected their medical conditions which have an impact on incrasing blood pressure. The method used in the research is case study method with qualitative approach. The partisipan that involved was one person (N=1). A man 55 years who served as a traditional village leader and carried out duties in village management and suffered high blood pressure. Data was collected by using interview asessment techniques, observation, and also using several psychological instruments to make it easier arrange the psychological dynamic of the participant. The results of this study is the increase of blood pressure experienced by participant is influenced by the presense of strong stressor from dthe social environment.

Low Survivals and Rapid Demographic Decline of a Threatened Estuarine Delphinid

Beibu Gulf’s (BBG) Indo-Pacific humpback dolphins present both a genetic differentiation and phenotypical differences from conspecifics from other areas of the South China Sea. Given the recent urbanization and industrialization in southern China, humpback dolphins from the BBG warrant conservation attention. However, this population’s demographic trend is unclear, making it hard to take conservation measures. To assess the population status of humpback dolphins in the BBG, photo-identification surveys were conducted between 2015 and 2019 in the inshore region surrounding the Dafeng River Estuary, which represents the most urbanized and industrialized coastal area of the BBG region. Robust design modeling suggested a constant survival for the female adults (0.89, 95% CI: 0.83–0.94). In comparison, the survival of the juvenile and sex-undetermined adults dropped from 0.92 (95% CI: 0.75–0.98) in 2015 to 0.86 (95% CI: 0.71–0.94) in 2016 and bounced back to 0.89 (95% CI: 0.80–0.94) in 2018. The low level of survival may justify the rapid decline in the annual population size from 156 (95% CI: 133–184) in 2015 to 102 (95% CI: 98–107) in 2019. We found little impact of emigration on the dolphin demographic process. Instead, the low and fluctuating survivals, although with overlapping confidence intervals, seemingly suggested a presence of strong marine stressor(s). Our study highlighted that obtaining high-resolution data is essential to improving our understanding of the demographic dynamics. Moreover, the anthropogenic stress in the BBG region should be quantitatively studied in both temporal and spatial perspectives, to help depict the ecological response of the dolphins to anthropogenic activities.

The Butterfly Effect: Mild Soil Pollution with Heavy Metals Elicits Major Biological Consequences in Cobalt-Sensitized Broad Bean Model Plants.

Among the heavy metals (HMs), only cobalt induces a polymorphic response in Vicia faba plants, manifesting as chlorophyll morphoses and a ‘break-through’ effect resulting in the elevated accumulation of other HMs, which makes Co-pretreated broad bean plants an attractive model for investigating soil pollution by HMs. In this study, Co-sensitized V. faba plants were used to evaluate the long-term effect of residual industrial pollution by examining biochemical (H2O2, ascorbic acid, malondialdehyde, free proline, flavonoid, polyphenols, chlorophylls, carotenoids, superoxide dismutase) and molecular (conserved DNA-derived polymorphism and transcript-derived polymorphic fragments) markers after long-term exposure. HM-polluted soil induced a significantly higher frequency of chlorophyll morphoses and lower levels of nonenzymatic antioxidants in Co-pretreated V. faba plants. Both molecular markers effectively differentiated plants from polluted and control soils into distinct clusters, showing that HMs in mildly polluted soil are capable of inducing changes in DNA coding regions. These findings illustrate that strong background abiotic stressors (pretreatment with Co) can aid investigations of mild stressors (slight levels of soil pollution) by complementing each other in antioxidant content reduction and induction of DNA changes.

Effects of β-1,3-glucan and ascorbic acid on the nutritional-immune response and antioxidant signaling pathways of live tiger grouper during simulated transport

Transport in water is the most common method for transporting live fish in China, however, transport is a strong stressor. Transport stress could lead to a reduced immune and antioxidant system function of tiger grouper, resulting in sickness and death. Besides, tiger grouper were continuously stressed during transport, which resulted in quality deterioration. It is necessary that find a way to relieve the stress of transportation of tiger grouper. Ascorbic acid is not only a good anti-stress agent, but it is also an effective immunostimulant. β-1,3-glucan is a feed additive that can enhance the immune response of fish. Therefore, this study evaluated the effects of β-1,3-glucan and ascorbic acid on the nutritional-immune response and antioxidant signaling pathways of live tiger grouper during simulated transport. Results indicated that adding β-1,3-glucan and ascorbic acid in transport-water muted the increase of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activity. In addition, β-1,3-glucan and ascorbic acid activated Nrf2 and mediated TOR expression and then up-regulate related mRNA expression of antioxidant and immune enzymes. We concluded that the application of β-1,3-glucan and ascorbic acid inhibit the increase of metabolism enzymes and inflammatory factors and activate immune and antioxidant signaling pathways to relieve oxidant stress, immune response, and apoptosis. Reducing the loss of amino acids provided nutrients to relieve oxidative stress and immune response, which demonstrated immune-nutritional response in live tiger grouper during simulated transport. These results may provide a new solution for alleviating the decline of immune and antioxidant function of tiger grouper caused by transportation stress.

High, in Contrast to Low Levels of Acute Stress Induce Depressive-like Behavior by Involving Astrocytic, in Addition to Microglial P2X7 Receptors in the Rodent Hippocampus.

Extracellular adenosine 5′-triphosphate (ATP) in the brain is suggested to be an etiological factor of major depressive disorder (MDD). It has been assumed that stress-released ATP stimulates P2X7 receptors (Rs) at the microglia, thereby causing neuroinflammation; however, other central nervous system (CNS) cell types such as astrocytes also possess P2X7Rs. In order to elucidate the possible involvement of the MDD-relevant hippocampal astrocytes in the development of a depressive-like state, we used various behavioral tests (tail suspension test [TST], forced swim test [FST], restraint stress, inescapable foot shock, unpredictable chronic mild stress [UCMS]), as well as fluorescence immunohistochemistry, and patch-clamp electrophysiology in wild-type (WT) and genetically manipulated rodents. The TST and FST resulted in learned helplessness manifested as a prolongation of the immobility time, while inescapable foot shock caused lower sucrose consumption as a sign of anhedonia. We confirmed the participation of P2X7Rs in the development of the depressive-like behaviors in all forms of acute (TST, FST, foot shock) and chronic stress (UCMS) in the rodent models used. Further, pharmacological agonists and antagonists acted in a different manner in rats and mice due to their diverse potencies at the respective receptor orthologs. In hippocampal slices of mice and rats, only foot shock increased the current responses to locally applied dibenzoyl-ATP (Bz-ATP) in CA1 astrocytes; in contrast, TST and restraint depressed these responses. Following stressful stimuli, immunohistochemistry demonstrated an increased co-localization of P2X7Rs with a microglial marker, but no change in co-localization with an astroglial marker. Pharmacological damage to the microglia and astroglia has proven the significance of the microglia for mediating all types of depression-like behavioral reactions, while the astroglia participated only in reactions induced by strong stressors, such as foot shock. Because, in addition to acute stressors, their chronic counterparts induce a depressive-like state in rodents via P2X7R activation, we suggest that our data may have relevance for the etiology of MDD in humans.

Mental Health Problems of the People in Isolation Related to SARS-CoV-2 Virus Infection

Objectives: To define the mental health status of people in isolation, and determined the correlation between mental health variables of the respondents. Methods: The study design was descriptive cross-actional. Isolated citizens, who were transported by charter flight from different foreign countries to Mongolia from July 1, 2020, to September 1, 2020, and observed by epidemiological reference in the isolation places. Results: The majority (38.8%, n = 157) of the 405 total cases aged 17- 97 year-olds, were 25- 34 year-olds and the average age was 34.2 ± 14.6. Majority of respondents (n = 213; 52.6%) were male. When mental health variables were compared with age groups, depression, anxiety, and self-stigma were not statistically significant for age group. However, in any age group of participants, self-stigma was moderately more at 91.8% (p = 0.043), and self-stigma at 93.8% was moderate. 38.1% of the total isolated population had mild to severe depression, and 30.4% had mild to moderate anxiety. Conclusion: Respondents who were impacted by strong stressors were more likely to experience depression and anxiety than respondents who were not impacted, and increased levels of depression were caused by strong stressors and anxiety. A moderate level of self-stigma is more present in any age group.

Pyriproxyfen exposure induces DNA damage, cell proliferation impairments and apoptosis in the brain vesicles layers of chicken embryos

Larvicide pyriproxyfen (PPF), used in drinking water reservoirs to control Aedes mosquitoes, has already been shown as a possible cause of congenital anomalies in the central nervous system. However, the neurotoxic effects of PPF on the development of vertebrate embryos are still underexplored. Thus, the aim of this study was to investigate the effects of PPF on the morphometric parameters of the head and brain, as well as on the cell layers of the forebrain and midbrain, using embryos of Gallus domesticus as a model. Two sublethal PPF concentrations (0.01 mg/L and 10 mg/L), as defined by a survival curve, were tested. Analysis of the biometry of embryos showed significant reduction in body and brain mass and also in measurements of the head and brain. A reduction in cell layer thickness of the forebrain and midbrain was observed, accompanied by a reduction in the numerical density of cells per area. Changes in brain and head sizes and in the thickness of the cell layers of the forebrain and midbrain were significant at 10 mg/L PPF. Notably, PPF caused DNA doublestrand breaks and induced apoptosis in embryos exposed to 10 mg/L, which were accompanied by a reduction in cell proliferation. Regarding neuronal and glial differentiation, no changes were observed in the number of neurons and glial cells on the analyzed layers. Furthermore, PPF did not impact the head ossification process. These findings reveal that PPF is a strong stressor for neurodevelopment, causing damage to the cell architecture of brain vesicles.

Governança de água e planejamento urbano: aproveitamento de água de chuva para construção de cidades mais resilientes

Climate change and the increase in the urban population are strong stressors for water resources, making it necessary to discuss the construction of urban systems that are resilient and sensitive to water. In this way, the use of rainwater presents itself as an important alternative for accessing water and reducing pressure in water sources. This research aimed to identify the potential of rainwater harvesting in thirteen cities in the State of Paraiba by means of computer simulations. Twelve scenarios were adopted for each city and it was found that the capture of rainwater in the study sites is feasible to decrease the demand for drinking water, corroborating for the decentralized water supply and the increase of urban resilience. Finally, it is understood that, when added to urban planning, this technology tends to favor less hydric vulnerable cities.

Effects of the COVID-19 pandemic on perceived stress levels of employees working in private organizations during lockdown

Background Worldwide, office employees have experienced two novel phenomena during the COVID-19 pandemic and lockdown: working from home and reductions in their salaries. These phenomena have majorly impacted the lives of individuals as well as their families. The present study aims to measure the perceived stress levels among employees during the COVID-19 pandemic lockdown in Pakistan. Methodology A cross-sectional approach was used to collect data from a sample of 385 employees working in three different IT-oriented private organizations. A semi-structured, Likert-scale questionnaire was used, containing the 10-item inventory from the Perceived Stress Scale (PSS). A sociodemographic profile, working parameters, and frequency of PSS inventory were presented in the descriptive analysis. Binary logistic regression analysis was made to compare the stress levels of the two groups resulting from their workloads and reductions in salary. Results An elevated perceived stress level (50.9%) was observed among the employees, showing a significant relationship with salary reduction and home work engagement (p<0.005). A strong negative correlation was observed (-0.860) for the salary reduction with PSS. Conclusion Working from home and reductions in salary caused increased perceived stress levels of the employees working in private organizations. Salary reductions proved to be a strong stressor, causing economic disruption and inability to cope with financial burdens during the COVID-19 pandemic.

Environmental hormesis of non-specific and specific adaptive mechanisms in plants

Adaptive responses of plants are important not only for local processes in populations and communities but also for global processes in the biosphere through the primary production of ecosystems. In recent years, the concept of environmental hormesis has been increasingly used to explain the adaptive responses of living organisms, including plants, to low doses of natural factors, both abiotic and biotic, as well as various anthropogenic impacts. However, the issues of whether plant hormesis is similar/different when it is induced by mild stressors having different specific effects and what is the contribution of hormetic stimulation of non-specific and specific adaptive mechanisms in plant resilience to strong stressors (i.e., preconditioning) remains unclear. This paper analyses hormetic stimulation of non-specific and specific adaptive mechanisms in plants and its significance for preconditioning, the phenomenon of the hormetic trade-off for these mechanisms, and the position of hormetic stimulation of non-specific and specific adaptive mechanisms in the system of plant adaptations to environmental challenges. The analysis has shown that both non-specific and specific adaptive mechanisms of plants can be stimulated hormetically by mild stressors and are important for plant preconditioning. Due to limited plant resources, non-specific and specific adaptive mechanisms have hormetic trades-offs 1 (hormesis accompanied by the deterioration of some plant traits) and 2 (hormesis of some plant traits with the invariability of others). At the same time, hormetic trade-off 2 is observed much more often than hormetic trade-off 1, at least, this was demonstrated here for non-specific adaptive responses of plants. The hormetic stimulation of non-specific and specific adaptive mechanisms is part of the inducible adaptation of plants caused by stress factors and is an adaptation to random (unpredictable) changes in the environment.